CN109055212B - Multi-component coaxial printing nozzle - Google Patents
Multi-component coaxial printing nozzle Download PDFInfo
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- CN109055212B CN109055212B CN201810875374.6A CN201810875374A CN109055212B CN 109055212 B CN109055212 B CN 109055212B CN 201810875374 A CN201810875374 A CN 201810875374A CN 109055212 B CN109055212 B CN 109055212B
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Abstract
The invention discloses a multi-component coaxial printing spray head which comprises a coaxial air pressure spray head main body and a multi-component positioning assembly, wherein the coaxial air pressure spray head main body is fixedly provided with an inner spray head and an outer spray head; a pore passage for the inner spray head to pass through is arranged in the coaxial air pressure spray head main body, and an air inlet channel is formed between the outer wall of the inner spray head and the inner wall of the pore passage; and the coaxial air pressure nozzle body is also provided with an air inlet communicated with the air inlet channel. The multi-component coaxial printing nozzle has the characteristics of good biocompatibility, small design size, convenience in disassembly, assembly, sterilization and cleaning, easiness in integration, high stability, multi-component adjustability and the like, and has excellent prospects in the research fields of cell medicine research, in-vitro organoid research, three-dimensional tissue printing and the like.
Description
Technical Field
The invention relates to the technical field of 3D printing, in particular to a multi-component coaxial printing nozzle.
Background
With the increasing emphasis on self-health and disease research, tissue engineering and regenerative medicine have come to the rapid development stage in the past decade. Among them, with the advent of cell printing and the report of related research results, the topic of biological manufacturing has attracted more and more attention and attention. The discovery of new materials and the invention of a novel manufacturing process enable researchers to carry out targeted treatment aiming at more and more extensive surgical diseases, realize the regeneration of damaged tissues and organs, and greatly improve the disease treatment capability of human beings and the happiness of the whole people.
The most widespread basis of clinical medicine research is human cells, and great progress has been made in the past few years for printing and in-vitro assembly of living cells, wherein the extrusion cell printing process has the advantages of cheap nozzle equipment, simple forming principle and high expansibility. The method has the characteristics of simple principle, high process integration level, high operability and the like, and is greatly concerned and adopted. It is a common cell printing method at present to print living cells mixed with different sources or with specific functions into three-dimensional entities with specific shapes and functions by means of extrusion rapid prototyping. The manufacturing process can be used for manufacturing bionic organs, tissue scaffolds, cell medicines and the like, and is primarily applied to the fields of medicine screening, cell physiology research, bone repair, tissue regeneration, stem cell research, gene medicines and the like.
In the extrusion molding process, usually, the surface tension between the printing material and the nozzle can cause the size of the extruded liquid drop to be far larger than the diameter of the nozzle under the condition of no external force, and the molding precision of the technology is greatly limited. Reducing the minimum printable dimension by reducing the diameter of the nozzle or changing the viscosity of the material can lead to reduced cell viability and disqualification of the physical and biological properties of the material, ultimately resulting in failure of the formation.
The coaxial air pressure nozzle can overcome the surface tension at the tail end of the nozzle through air flow shearing, the extrusion precision of the material can be greatly improved, and micro-injection of biological ink and accurate wrapping of single cells are realized. The conventional coaxial air pressure nozzle has the capability of jetting micro liquid drops with the diameter of less than 300 mu m through the coaxial nozzle structure and the optimization of printing parameters. The conventional coaxial nozzles for micro-droplet ejection mainly include two coaxial nozzles, the outer one of which applies an air flow and the inner one of which is loaded with bio-ink containing cells. The size of the printed micro-liquid drop can be controllably adjusted by changing the diameter of the coaxial spray head and the size of the shearing air flow.
At present, some researchers at home and abroad design a process for realizing gel micro-droplet injection by using a coaxial nozzle. Li et al studied the air-jet of sodium alginate gel droplets using a conventional T-shaped coaxial nozzle, and explored the rule of gel liquid size control by varying experimental parameters such as extrusion flow rate, coaxial air pressure, and sodium alginate gel concentration (Ye B, Xu H, Bao B, et al.3D-printed air-blast microfluidic nanoparticles for preparing calci μm alginate microparticles [ J ]. Rsc Advances,2017,7(77): 48826-48834.). The coaxial nozzle is manufactured through 3d printing, and has the advantages of integration and simple structure. However, in order to improve the rigidity of the spray head, a coaxial liner is added inside the spray head. The stability of the flow field inside the coaxial nozzle is influenced to a certain extent. Ronald et al designed metallic triple coaxial nozzles (Si T, Xu R. design and characterization of tri-axial piping and materials in flow focusing for micro-encapsulation of multiple nozzles and imaging agents [ J ]. Proceedings of SPIE-The International Society for Optical Engineering,2014,8976(2): 197-203.). The nozzle is nested in a series form in a first-level mode, and the silica gel pad is used for sealing. The design is also a T-shaped structure, the metal dispensing needle head is directly drilled with holes and welded with a metal capillary, and the installation of the liquid through port of the outer side spray head is realized. In addition, in order to ensure the coaxiality of the coaxial spray heads, welding points are added on the surface of the needle head of the inner spray head and are used for being in contact positioning with the outer spray head. The spray head has the disadvantages that each stage of spray head needs to be welded, the manufacturing difficulty is high, and the spray head cannot be replaced and matched at will.
In the existing inventions and devices of some coaxial air pressure nozzles, the effect of air flow shearing on reducing the size of sprayed liquid drops is basically realized, but the coaxiality of the inner nozzle and the outer nozzle cannot be ensured. Some existing methods for ensuring the coaxiality of the inner nozzle and the outer nozzle are usually to add a limiting structure between the two nozzles, so that the stability of middle air flow is greatly influenced, and the precision of the spraying position of micro liquid drops is influenced. Moreover, the prior inventions and devices do not employ multi-component jetting control, and lack real-time control of multiple material loading and heterogeneous distribution of printed structures. Often only biological structures of uniform composition can be produced. Therefore, there is a need for a coaxial spray head having a stable pneumatic spray environment and a precisely adjustable multi-component tip.
Disclosure of Invention
The invention provides a multi-component coaxial printing spray head, which can extrude at least two different gels containing cells or other components into an inner spray head through independent non-liquid inlet channels, realizes printing of multiple components, omits a coaxial adjusting component, and has simple structure and low manufacturing difficulty.
The invention can be used as a core mounting spray head for three-dimensional cell printing and realizes multi-component adjustable gradient printing and high-precision micro-printing.
A multi-component coaxial printing spray head comprises a coaxial air pressure spray head main body fixed with an inner spray head and an outer spray head, and a multi-component positioning assembly, wherein at least two liquid inlet channels in sealed butt joint with the inner spray head are arranged in the multi-component positioning assembly; a pore passage for the inner spray head to pass through is arranged in the coaxial air pressure spray head main body, and an air inlet channel is formed between the outer wall of the inner spray head and the inner wall of the pore passage; and the coaxial air pressure nozzle body is also provided with an air inlet communicated with the air inlet channel.
Preferably, the number of the air inlets is two or more, and the air inlets are symmetrically arranged along the circumferential direction of the air inlet channel.
The invention leads symmetrical air flow into the side wall of the main body of the coaxial air pressure spray head, and forms annular air flow to generate air flow shearing force to liquid drops at the tail end of the inner side spray head through a middle gap between the outer side needle head and the inner side needle head of the spray head so as to realize the quick spray of the liquid drops and the size control of tiny liquid drops.
Preferably, the multicomponent positioning assembly comprises:
a luer connector in plug fit with one end of the inner spray head;
at least two hollow capillaries disposed within the luer fitting, the hollow capillaries defining the inlet passage.
The invention adopts the Ruhr joint to facilitate the installation of the multi-component positioning assembly and also facilitates the adjustment of the axial position of the inner spray head. Metal luer fittings are typically used. The hollow capillary tube is generally made of stainless steel capillary tube.
The multi-component positioning assembly adopts a metal luer connector and a stainless steel hollow capillary tube, and the position is fixed through the solidification of resin. The hydrogel material enters the inner spray head of the coaxial air pressure spray head through the respective stainless steel capillary feed inlet, and a plurality of components are separated by utilizing the laminar flow effect to produce the liquid with the heterostructure. As a further preferable example, a photosensitive resin having biocompatibility is used as the resin.
Preferably, the hollow capillary tube has an outer diameter of 0.7mm and an inner diameter of 0.4mm, and is connected with a syringe pump or other liquid inlet device through a medical silicone tube with an inner diameter of 0.5 mm.
Preferably, the coaxial pneumatic nozzle body includes:
a positioning seat provided with the pore channel and the air inlet;
the top cover is matched with the positioning seat to axially position the inner spray head in the pore channel, and a through hole for inserting one end of the multi-component positioning assembly is formed in the top cover;
the bottom of the positioning seat is provided with an installation part for fixing the outer spray head.
The positioning seat is a main positioning part of the multi-component coaxial printing spray head and provides mounting support for other parts. In the invention, a gap is generally reserved between the multi-component positioning assembly and the through hole on the top cover, thereby providing convenience for adjusting the inner spray head.
The inner spray head of the present invention may be of the conventional stainless steel metal needle construction and generally comprises a needle portion and a base portion. The base portion is generally provided with a truncated cone-shaped configuration.
Preferably, an inner sprayer mounting cavity used for accommodating the inner sprayer base part is arranged in the positioning seat, the bottom of the inner sprayer mounting cavity is communicated with the pore channel, and a conical hole structure is arranged at the connecting part of the inner sprayer mounting cavity and the pore channel. The conical hole structure is matched with the circular truncated cone-shaped structure part of the inner spray head base part and is used for assisting in realizing axial adjustment of the inner spray head.
The coaxial pneumatic spray head is understood as a solid component, an air flow channel is arranged in the coaxial pneumatic spray head, the inner spray head is also arranged in the longitudinal air flow channel (or an air inlet channel), in order to carry out sealing and keep the axial positioning of the inner spray head and the coaxial pneumatic spray head, preferably, a silica gel sealing ring is arranged between the inner spray head and the coaxial pneumatic spray head, and the contact structure is a conical hole. The silica gel sealing ring is generally sleeved on the outer wall of the inner spray head base.
The coaxial pneumatic sprayer main body is formed by butting a top cover and a positioning seat, wherein the top cover is provided with a groove structure for connecting with the positioning seat, and the top cover is rotated to realize quick connection and separation with a cavity.
The top cover and the positioning seat can adopt various fixing structures, such as threaded fixing, clamping fixing, bonding fixing and the like. Preferably, a clamping mechanism for fixing the top cover and the positioning seat is arranged between the top cover and the positioning seat. Preferably, the bottom of the top cover is provided with a clamping groove, and the outer wall of the positioning seat is provided with a positioning bulge. Preferably, the clamping groove is provided with an extending groove section arranged along the circumferential direction of the top cover at a position close to the bottom of the groove, and the positioning protrusion on the positioning seat is clamped in the extending groove section in a locking state. The positioning bulge is preferably a fan-shaped bulge, and the size of the fan-shaped bulge is matched with the size of the notch of the clamping groove so as to facilitate the fan-shaped bulge to enter the clamping groove.
Preferably, the mounting portion has a columnar structure or a circular truncated cone structure that can be engaged with the outer head. The invention realizes the fixation of the outer spray head by using the plug structure, thereby facilitating the replacement and installation of the outer spray head and simultaneously facilitating the coaxial adjustment of the inner spray head and the outer spray head. Preferably, the columnar structure of the tail end of the coaxial air pressure spray head main body connected with the outer spray head adopts an international standard size, the dispensing needle head adopts a plastic base needle head, and the inner diameter of the dispensing needle head is larger than the outer diameter of the inner spray head.
Preferably, a luer connector in threaded fit is arranged in the air inlet. Preferably, the diameter of the vent hole at the end of the air inlet communicated with the pore channel is 1 mm. The number of the air inlets is preferably three. Three symmetrical luer connectors are used for introducing airflow of the same air source, and the air source needs to be filtered through a biological filter with the specification of 0.25 um.
In the invention, preferably, the top cover and the positioning seat are made of photosensitive resin with biocompatibility and are manufactured by adopting a 3D printing technology.
The printing material aimed by the invention can be a biological hydrogel material with biocompatibility for cell culture and tissue construction, and contains living cells for experiments, nutrient solution, growth factors and the like.
The hydrogel can be mixed with a certain amount of specific reagents for cell screening or dyeing and other reagents for experiment and auxiliary material forming, the hydrogel material is in a liquid phase before entering the front end of the multi-component mixing, and is subjected to phase change and fixed forming through chemical crosslinking or other physical conditions after being sheared and sprayed to a forming platform or the reagents by air flow. In order to ensure that the hydrogel reagent containing cells is not polluted by the outside, the whole device needs to be disassembled in advance, and conventional biological instrument disinfection operations such as alcohol disinfection, ultraviolet irradiation sterilization, deionized water cleaning and the like are carried out on all the components. Hydrogel materials for deployment also need to be sterilized and disinfected.
The metal materials adopted by the invention are all materials with excellent clinical biocompatibility or metal materials which are easy to sterilize and disinfect at high temperature, and the manufactured coaxial air pressure nozzle needs to be sterilized, disinfected and stored aseptically. The spray head needs to be cleaned and pre-extruded before the spray head starts to work.
Compared with the prior art, the invention has the following advantages:
(1) the coaxial air pressure nozzle adopts a modular design, so that the assembly and disassembly and sterilization cleaning are convenient;
(2) any obstacle structure used for limiting is not arranged between the inner spray head and the outer spray head of the coaxial air pressure spray head, so that the coaxial air passage is smoother, and the stability of air flow is improved.
(3) The coaxial air pressure nozzles are symmetrical in introduced air flow, so that the coaxial air flow is more stable, and the position precision of injection is greatly improved;
(4) the multi-component positioning assembly can load various component materials at the same time, so that the printing of a heterostructure is realized;
(5) the inner and outer nozzles of the coaxial air pressure nozzle can be replaced, so that the coaxial air pressure nozzle is suitable for printing in different occasions with different requirements;
(6) the coaxiality of the inner spray head and the outer spray head can be conveniently adjusted by adjusting the position of the outer spray head arranged at the tail end of the coaxial air pressure spray head.
(7) The coaxial air pressure nozzle body adopts a 3D printing process, so that the structure integrity is strong, and the cost is low.
(8) The coaxial pneumatic spray head main body adopts a structure of the top cover and the positioning seat, and is connected in a clamping manner, so that the coaxial pneumatic spray head is simple and convenient to design and is convenient to disassemble, assemble and sterilize.
In a word, the multi-component coaxial printing nozzle has the characteristics of good biocompatibility, small design size, convenience in disassembly, assembly, sterilization and cleaning, easiness in integration, high stability, adjustability of multiple components and the like, and has excellent prospects in the research fields of cell medicine research, in-vitro organoid research, three-dimensional tissue printing and the like.
Drawings
Fig. 1 is a schematic structural diagram of a multi-component coaxial print head of the present invention.
Fig. 2 is a cross-sectional view of a multi-component coaxial print head of the present invention.
Fig. 3 is a cross-sectional view of a plural component positioning assembly of the present invention.
Fig. 4 is a schematic structural view of the top cover of the present invention.
Fig. 5 is a schematic structural view of the coaxial pneumatic showerhead chamber of the present invention.
Fig. 6 is a cross-sectional view of a coaxial pneumatic showerhead chamber of the present invention.
Fig. 7 is a top view of a coaxial pneumatic showerhead chamber of the present invention.
In the figure: the device comprises a base, a pressure sensor, a pressure sensors, a pressure sensor, a pressure sensors, a pressure sensor, a pressure sensors, a pressure.
Detailed Description
Referring to fig. 1-7, the multi-component coaxial printing nozzle comprises a multi-component positioning assembly and a coaxial pneumatic nozzle body, wherein the multi-component positioning assembly is mounted at the top of the coaxial pneumatic nozzle body.
As shown in fig. 1 to 3, the multi-component positioning assembly includes a connector with a through hole structure and two or more liquid inlet pipes, in this embodiment, the connector is a metal luer connector 9, the liquid inlet pipe is a stainless steel hollow capillary 1, the two stainless steel capillary pipes are bent, the bending angle is 120 degrees, and the inner diameter is 400 μm. The number of the liquid inlet pipes can be determined according to actual needs and is generally determined by the type of materials to be printed. The two stainless steel hollow capillary tubes 1 are symmetrically distributed in the through hole of the metal luer 9 and fixed through the photosensitive resin 2, and the bottoms of the stainless steel hollow capillary tubes 1 are flush with the bottom of the luer 9, and finally the two stainless steel hollow capillary tubes are combined to form the multi-component positioning assembly.
The bottom of a stainless steel hollow capillary tube in the multi-component positioning assembly is flush with the Ruhr joint so as to conveniently realize the sealing butt joint with the inner spray head;
the coaxial pneumatic spray head main body mainly comprises a positioning seat 6, a top cover 8, an inner spray head 3 and an outer spray head 5.
The positioning seat 6 is a main supporting and positioning part of the whole coaxial sprayer and provides a mounting space or a mounting position for each part. 6 tops of positioning seat are equipped with interior shower nozzle installation cavity 6a, and 6 middle parts of positioning seat and lower part center are equipped with the pore 6b that link up with interior shower nozzle installation cavity, and 6 middle parts of positioning seat are equipped with one or more air inlets 6c all around, and when setting up a plurality of air inlets, a plurality of air inlets evenly arrange along positioning seat circumference, adopt evenly to arrange, have further guaranteed the stability of air current circumference. An air inlet joint is arranged in the air inlet 6c close to the outer end, and the inner end is communicated with the pore passage 6 b. The air inlet joint is an air inlet luer joint 7. The diameter of the pore channel is 1mm, the diameter of the communication part between the three external air inlets 6c and the pore channel is only 1mm, the smaller the air channel is, the smaller the air pressure can be, and the more stable the injection control of the micro-droplets is. The connection part of the nozzle installation cavity 6a and the pore passage 6b is provided with a taper hole structure. An air inlet channel is formed between the outer wall of the needle part of the inner spray nozzle and the inner wall of the pore channel.
Referring to fig. 4, the top cover 8 is arranged on the top of the positioning seat 6, a through hole 11 is arranged in the middle of the top cover, and the diameter of the through hole is smaller than the size of the end face of the inner spray head and used for limiting the inner spray head. The top cover 8 and the positioning seat 6 are quickly mounted and dismounted through a buckle, and the clamping groove 10 is matched with the fan-shaped boss 12 in a fastening state. Specifically, the side wall of the top cover 8 is provided with a clamping groove 10, the bottom of the clamping groove 10 is provided with an extension groove section 10a along the circumferential direction of the side wall of the top cover 8, and the extension groove section 10a is used for realizing final locking. The outer wall circumference in 6 tops of positioning seat evenly is equipped with a plurality of fan-shaped bosss 12, and the width of fan-shaped boss 12 cooperates with the notch width of draw-in groove 10, guarantees that top cap 8 can block into smoothly. In the embodiment, two fan-shaped bosses which are symmetrical at 180 degrees are arranged at the top end of the coaxial nozzle main body and are used for being installed with the top cover; the tail end of the coaxial sprayer body is provided with a columnar structure for installing the outer sprayer.
During actual installation, 8 openings of the top cover are covered downwards at the top of the positioning seat 6, the notch of the clamping groove 10 is aligned to the fan-shaped boss 12, after the fan-shaped boss 12 goes deep into the clamping groove 10, the fan-shaped boss 12 and the extension groove section 10a are located at the same height, the top cover 8 is rotated, the fan-shaped boss 12 is clamped into the extension groove section 10a, and finally mutual positioning between the top cover and the positioning seat is achieved.
When installed, the inner nozzle 3 is further passed through the orifice via the inner nozzle installation chamber 6 a. The inner cup is then axially confined within the positioning socket 6 by means of the top cover. Luer 9 of the multi-component positioning assembly is connected to inner nozzle 3 mounted inside the body of the coaxial pneumatic nozzle through hole 11 of cap 8.
The size of the top through hole 11 of the top cover 8 is smaller than the maximum size of the base of the inner spray head 3, and after the top cover 8 is fastened with the positioning seat 6, the inner spray head 3 is limited in position in the vertical direction.
The cone structure on the base of the inner spray head 3 is matched with the taper hole in the hole channel of the positioning seat 6, and the taper of the cone is larger than that of the taper hole. After installation, the air passage and the upper structure are sealed through the silica gel sealing ring 4 (the silica gel sealing ring 4 is sleeved on the round platform structure of the inner spray head 3).
The side wall of the positioning seat 6 is provided with three circumferentially symmetrical threaded holes for mounting the luer connector 7, and a lateral mounting plane 14 is arranged at the mounting position for positioning and sealing the end face.
Referring to fig. 5-7, a circular truncated cone structure 13 is arranged at the bottom of the positioning seat 6, and the circular truncated cone structure adopts international standard size and is used for being rapidly connected with the outer spray head 5. The length of the extending part of the needle head of the outer spray head 5 is less than 10mm, on one hand, the processing of the outer spray head and the inner spray head is convenient, and meanwhile, the deviation of the inner spray head caused by the length process is further avoided.
The bottom ends of the inner spray head and the outer spray head can be parallel and level, and a small height difference can be set so as to meet the requirements of different processes.
The multi-component coaxial printing nozzle is arranged on the three-dimensional moving platform, and can realize accurate printing of three-dimensional cells. Before printing, the inner and outer pinheads with reasonable specifications are selected in advance, and the shearing air pressure is adjusted to a reasonable value. Wherein, a channel for outputting printing materials is formed in the inner spray head, and a gap between the inner spray head and the pore canal forms a channel for outputting gas.
In the invention, a gap is arranged between the Ruhr joint 9 and the through hole of the top cover 8, the angle between the Ruhr joint and the central axis of the positioning seat is slightly changed, the angle adjustment of the inner spray head can be realized, and the inner spray head is the basis for the position adjustment of the inner spray head in the coaxial adjustment process. Meanwhile, the outer spray head and the cavity are mutually fixed through a direct plugging structure, so that certain angular offset is allowed to be adjusted.
When the inner spray head is installed, the inner spray head is fixed by the top cover 8 and the tapered hole in the vertical direction, and the inner spray head is initially adjusted through the tapered hole to approximately approach to the central position; then the outer nozzle is finely adjusted (which can be done under a magnifying microscope) so that the inner and outer nozzles are coaxial.
The multi-component coaxial printing nozzle provided by the invention has the following use process:
the three Ruhr joints 7 are connected into the same clean gas source, gas is generated by the gas pump and is adjusted by the gas pressure controller, and in order to improve the adjustment precision of the gas pressure, the gas from the gas pressure controller can be further finely adjusted by a pressure reducing valve; the gel material containing cells of a plurality of components enters the inner spray head 3 through different material inlet ports of the multi-component positioning assembly to realize spraying, and the heterostructure printing with different component distribution ratios can be realized through a precise injection pump or other precise feeding devices and through specific control strategies of users in the process.
Specific examples are as follows:
the multi-component coaxial printing nozzle is used for manufacturing gel microspheres containing different cell ratios (human umbilical cord vascular endothelial cells and human mesenchymal stem cells) in a large batch, and the influence of the difference of the two cell ratios on the characterization of vascularization of the vascular endothelial cells and the calcification of the mesenchymal stem cells is researched.
Step one, preparing all parts, soaking a top cover, a positioning seat, a multi-component positioning assembly, a luer connector, a silica gel sealing ring, an inner spray head and an outer spray head in 75% medical alcohol in advance, sealing and wrapping the parts by a utensil, and placing the parts in an ultrasonic cleaning machine for treating for more than 10min to remove foreign matters such as a surface oxidation layer, an oil layer and the like. Then taking out and placing the mixture on a clean bench, repeatedly washing the mixture for three times by using deionized water, and airing the mixture. Irradiating organic material components such as a top cover, a positioning seat, a multi-component positioning assembly, a silica gel sealing ring and the like for more than 30min under the ultraviolet light of a sterile transfer value; and transferring the metal luer connector and the inner and outer spray heads to a high-temperature sterilization box for sterilization.
And step two, assembling all parts on the clean bench, and connecting the multi-component positioning assembly with an upper feeding silica gel hose. A clean gas source was connected to three symmetrical inlet luer fittings.
Step three, centrifuging the endothelial cell strain for experiment, stirring and mixing the endothelial cell strain and sodium alginate hydrogel with proper concentration at 37 ℃ uniformly to obtain a component A, and placing the component A into an injector; the mesenchymal stem cell strain for experiment is centrifuged and mixed with sodium alginate hydrogel with proper concentration at 37 ℃ to be uniformly stirred and mixed as a component B, and the component B is placed in an injector.
And step four, connecting an injector provided with two components with a feeding hose, pre-extruding the two channels simultaneously until hydrogel is extruded from the inner nozzle, and stopping pre-extruding.
Fifthly, controlling according to the previously arranged extrusion proportion, and spraying the sodium alginate micro-droplets; the sprayed sodium alginate hydrogel drops enter a receiving device below the spraying device and are mixed with 2% (w/v) CaCl in the spraying device2And carrying out chemical crosslinking on the solution to form the gel microspheres. The formed microspheres have component difference according to different extrusion ratios of two cells, so that gel microspheres with A: B of 0 to infinity in any ratio can be theoretically formed.
The steps are operated in a clean environment, and the sodium alginate gel microspheres formed by extrusion can be replaced by other types of cell tissue scaffolds.
The multi-component coaxial printing nozzle has the characteristics of small volume, convenience in installation, symmetrical structure and the like, and can realize heterogeneous printing of a plurality of component materials in different proportions; the adopted materials are all biocompatible materials, so that toxic damage to cells can not be caused; the size of micro liquid drops can be reduced by an air flow shearing mode, and the jetting precision is greatly improved. Meanwhile, the extrusion flow of the material is adjusted, so that the ejection frequency of the micro-droplets can be adjusted, and the method can be used for a high-flux microsphere manufacturing process and a low-flux micro-droplet cell ejection 3D printing process. Provides a good reference for cytomedicine research, in-vitro organoid research and three-dimensional tissue printing.
Claims (4)
1. A multi-component coaxial printing spray head comprises a coaxial air pressure spray head main body fixed with an inner spray head and an outer spray head, and is characterized by also comprising a multi-component positioning assembly, wherein at least two liquid inlet channels in sealed butt joint with the inner spray head are arranged in the multi-component positioning assembly; a pore passage for the inner spray head to pass through is arranged in the coaxial air pressure spray head main body, and an air inlet channel is formed between the outer wall of the inner spray head and the inner wall of the pore passage; the coaxial air pressure nozzle body is also provided with an air inlet communicated with the air inlet channel;
the number of the air inlets is two or more, and the air inlets are symmetrically arranged along the circumferential direction of the air inlet channel;
the coaxial pneumatic nozzle body includes:
a positioning seat provided with the pore channel and the air inlet;
the top cover is matched with the positioning seat to axially position the inner spray head in the pore channel, and a through hole for inserting one end of the multi-component positioning assembly is formed in the top cover;
the bottom of the positioning seat is provided with an installation part for fixing the outer spray head;
an inner spray head mounting cavity for accommodating the inner spray head base part is simultaneously arranged in the positioning seat, the bottom of the inner spray head mounting cavity is communicated with the pore channel, and a conical hole structure is arranged at the connecting part of the inner spray head mounting cavity and the pore channel;
the installation part is a columnar structure or a circular truncated cone structure which can be clamped with the outer spray head.
2. The multi-component coaxial print head of claim 1, wherein the multi-component positioning assembly comprises:
a luer connector in plug fit with one end of the inner spray head;
at least two hollow capillaries disposed within the luer fitting, the hollow capillaries defining the inlet passage.
3. The multi-component coaxial print head of claim 1 wherein a snap-fit mechanism is disposed between the cap and the positioning seat to secure the cap and the positioning seat.
4. The multi-component coaxial print head of claim 1, wherein a threaded luer fitting is provided in the gas inlet.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810875374.6A CN109055212B (en) | 2018-08-03 | 2018-08-03 | Multi-component coaxial printing nozzle |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810875374.6A CN109055212B (en) | 2018-08-03 | 2018-08-03 | Multi-component coaxial printing nozzle |
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| CN109055212A CN109055212A (en) | 2018-12-21 |
| CN109055212B true CN109055212B (en) | 2022-05-03 |
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| CN109929760A (en) * | 2019-04-08 | 2019-06-25 | 华子昂 | The preparation facilities and its application method of the hollow gelatinous fibre pipe of coaxial multi-layer and application |
| CN110101448B (en) * | 2019-04-22 | 2020-10-27 | 西安交通大学 | Photocuring hydrogel conveying device for minimally invasive surgery and in-situ printing method |
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| CN105772725B (en) * | 2016-04-06 | 2018-05-11 | 西安交通大学 | A kind of Efficient high-temperature nozzle for supporting more printed materials |
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| CN106891527B (en) * | 2017-02-14 | 2019-05-24 | 中国科学院苏州纳米技术与纳米仿生研究所 | 3D biometric print nozzle component, printing equipment and application for more materials |
| CN206579134U (en) * | 2017-03-31 | 2017-10-24 | 东北林业大学 | One kind coextrusion 3D printing shower nozzle |
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