CN116059517A - Biological ink spraying device - Google Patents

Abstract

The invention discloses a biological ink spraying device, which belongs to the field of medical instruments and comprises a shell, a fluid input assembly, a gas input part, a needle tube assembly and a flow limiting block, wherein the shell is provided with an inner hole, the fluid input assembly, the gas input part and the needle tube assembly are all arranged on the shell, the gas input part is communicated with the inner hole, the needle tube assembly comprises a needle tube, the needle tube is provided with a liquid runner, the liquid runner is communicated with the fluid input assembly, the flow limiting block is arranged on the shell, the flow limiting block is provided with a flow limiting part, the flow limiting block is provided with a through hole, the through hole penetrates through the flow limiting part, the flow limiting part is positioned in the inner hole, the tail end of the needle tube is positioned in the through hole, a gas runner is formed between the outer wall of the needle tube and the inner wall of the through hole, the gas runner is communicated with the inner hole, and the sectional area of the gas runner is smaller than that of the inner hole so that gas input by the gas input part accelerates the gas output by the needle tube to uniformly atomize.

Description

Biological ink spraying device

Technical Field

The invention relates to the field of medical instruments, in particular to a biological ink spraying device.

Background

Large-area skin wounds are now clinically common diseases, are often closely related to various emergencies or serious chronic diseases, have the characteristic of lasting disunion, have serious influence on the life quality of patients, are more serious and possibly endanger lives, and cause huge social and economic losses both at home and abroad. Cell spray autograft is an innovative method for early treatment of large-area deep second-degree burn wounds, namely, cells (such as keratinocytes) are derived from the skin, the required cell number only needs to come from a small donor part, and the cells are subjected to cell culture passage or are directly suspended in normal saline or other biological materials to prepare biological ink (Bio-ink) which is uniformly sprayed on the wounds to proliferate the cells and improve re-epithelialization. Compared with biological printing, the method has more advantages in cost, can realize portable operation, and has unique advantages in the early treatment of chronic incurable wounds such as large-area deep burn wounds, diabetic feet, skin ulcers and the like.

Currently, there are some published atomizers or spray systems based on cell spray autograft methods. The disclosed cell or drug spraying device (CN 201711409096, CN202023309697, CN 201810462736) adopts a piezoelectric printing nozzle, the method is difficult to spray and print the biological ink with the viscosity of more than 6 mPa.s, and under the severe vibration of an ultrasonic transducer, the cell suspension has the risk of rapid temperature rise, so that the cell activity after spraying is reduced. The disclosed cell suspension atomizer (CN 202020240040) employs a multi-mesh jet atomization method, which generally produces very large droplet sizes; to create an atomising effect, the driving hydraulic pressure of the cell suspension is very high, the cell viability is low, generally not higher than 80%; and it is difficult to atomize the bio-ink having a viscosity of more than 6mpa·s. The disclosed hydraulic swirl atomization drug delivery devices (CN 202122889314, CN201910934672 and CN 201921645190) have the defects of the atomizers, the activation rate after cell spraying is generally lower than 80%, and uniform spraying of high-viscosity biological ink is difficult to realize.

Disclosure of Invention

In order to overcome the defects in the prior art, one of the purposes of the invention is to provide a bio-ink spraying device for improving the activity and the cell differentiation efficiency after cell spraying and realizing the spraying of high-viscosity bio-ink.

One of the purposes of the invention is realized by adopting the following technical scheme:

the utility model provides a bio-ink spraying device, includes casing, fluid input subassembly, gas input spare, needle tubing subassembly and restriction block, the casing is equipped with the hole, fluid input subassembly gas input spare and needle tubing subassembly all install in the casing, gas input spare with the hole intercommunication, needle tubing subassembly includes the needle tubing, the needle tubing is equipped with the liquid runner, the liquid runner with fluid input subassembly intercommunication, restriction block install in the casing, restriction block is equipped with the restriction part, restriction block is equipped with the through-hole, the through-hole runs through restriction part, restriction part is located in the hole, the needle tubing end is located in the through-hole, the needle tubing outer wall with form the gas runner between the through-hole inner wall, the gas runner with the hole intercommunication, the cross-sectional area of gas runner is less than the cross-sectional area of hole makes the gas of gas input spare input is accelerated with the even atomizing of liquid of output.

Further, the needle tube assembly is detachably mounted to the housing.

Further, the needle tube assembly comprises a positioning block and a needle tube, and the needle tube is fixedly matched with the positioning block.

Further, the needle tube is in interference fit with the positioning block.

Further, the positioning block comprises a body and a flange extending from the body, the shell comprises a first installation portion and a main body, the first installation portion is detachably connected with the main body, the positioning block stretches into the main body, and the first installation portion abuts against the flange to enable the positioning block to be detachably installed in the shell.

Further, the fluid input assembly comprises an input joint and an input pipe arranged on the input joint, and the input joint stretches into the positioning block and is in threaded connection with the positioning block.

Further, the bio-ink spraying device further comprises a compression block, the compression block comprises an inserting connection part and a compression part, the tail end of the input pipe extends into the compression block, the inserting connection part is positioned between the outer wall of the input pipe and the inner wall of the input joint, the compression part is abutted to the input joint and the positioning block, and the compression block enables the input pipe to be in sealing connection with the needle tube.

Further, the flow limiting block is detachably mounted on the shell.

Further, the casing includes main part and second installation department, second installation department demountable installation in the main part, the restriction piece still is equipped with the base, restriction portion follows the base extends and goes out, the base includes conflict portion, conflict portion with second installation department conflict makes the restriction piece install in the casing.

Further, the base is provided with a bottom end face, the needle tube is provided with a distal end face, and the second mounting portion is in threaded connection with the main body so as to adjust the distance between the distal end face and the bottom end face.

Compared with the prior art, the biological ink spraying device has the following advantages that;

(1) The cross section of the gas flow channel is smaller than that of the inner hole, so that the gas input by the gas input part accelerates to uniformly atomize the liquid output by the needle tube, and the high-viscosity bio-ink can be uniformly sprayed by the design;

(2) The needle tube component is detachably arranged on the shell, and when biological ink with different viscosities is sprayed, the needle tube component can be conveniently replaced so as to correspond to the needle tubes with different diameters;

(3) The flow limiting block is detachably arranged on the shell, and can be conveniently replaced when biological ink with different viscosities is sprayed, so that the sectional area of the gas flow channel can be adjusted, and the gas spraying speed can be adjusted.

Drawings

FIG. 1 is a perspective view of a bio-ink spraying device according to the present invention;

FIG. 2 is an exploded view of the bio-ink spraying device of FIG. 1;

FIG. 3 is a perspective view of a needle cannula assembly of the bio-ink spraying device of FIG. 2;

FIG. 4 is a cross-sectional view of the bio-ink spraying device of FIG. 1;

FIG. 5 is an enlarged view of the bio-ink spraying device A of FIG. 4;

FIG. 6 is an enlarged view of the bio-ink spraying device B of FIG. 4;

FIG. 7 (a) is a single frame spray cone angle image during the spraying process of the bio-ink spraying device of the present invention;

FIG. 7 (b) is a superimposed image of a number of frames of spray cone angles;

fig. 7 (c) is a view of the spray angle image of fig. 7 (b) after binarization;

FIG. 7 (d) shows the near-field cone angle θ after least squares fitting of the boundaries in FIG. 7 (c) ₁ And far field cone angle theta ₂ A figure;

fig. 8 is θ of different spray objects ₁ A variation map;

fig. 9 is a view of θ of different spray objects ₂ A variation map;

FIG. 10 (a) is a diagram showing a test of a spraying state of a 1% (w/v) SA solution by a bio-ink spraying device at a flow rate of 1.0L/min of assist air;

FIG. 10 (b) is a diagram showing a test of a spraying state of a 1% (w/v) SA solution by the bio-ink spraying device at a flow rate of 1.5L/min of assist air;

FIG. 10 (c) is a diagram showing a spray status test of a 1% (w/v) SA solution by a bio-ink spray device at an assist air flow rate of 2.0L/min;

FIG. 10 (d) is a diagram showing a spray status test of the spraying device for a 1% (w/v) SA solution at an auxiliary air flow rate of 5.0L/min;

FIG. 11 is a graph showing the variation of the spray area of the bio-ink spraying device according to the present invention at different spray heights;

FIG. 12 (a) is a diagram showing a spraying state of a biological ink spraying device for quantitatively spraying 100. Mu.L;

FIG. 12 (b) is a diagram showing a spraying state of a bio-ink spraying device for quantitatively spraying 500. Mu.L;

FIG. 12 (c) is a graph showing the droplet distribution of the viscous solution expressed by a 1mL syringe needle;

FIG. 13 (a) is a graph showing the effect of spraying 1mL of 1% (w/v) SA on pigskin by a bio-ink spraying device;

FIG. 13 (b) is a graph showing the effect of spraying 2mL of 1% (w/v) SA on pigskin by a bio-ink spraying device;

FIG. 13 (c) is a graph showing the effect of spraying 1mL of physiological saline onto pigskin by the bio-ink spraying device;

FIG. 13 (d) is a graph showing the effect of spraying 2mL of physiological saline onto pigskin by the bio-ink spraying device;

FIG. 14 (a) shows a cell density of about 10 ⁵ The cell/mL solution is a 3D image which is spliced by three-dimensional scanning after 4 hours of biological ink spraying;

FIG. 14 (b) shows a cell density of about 10 ⁵ The cells/mL solution is a three-dimensional scanning spliced 3D image after the bio-ink is sprayed for 24 hours.

In the figure: 10. a housing; 11. a first mounting portion; 12. a main body; 13. a second mounting portion; 14. an inner bore; 20. a fluid input assembly; 21. an input connector; 22. an input tube; 221. a fluid input port; 30. a compaction block; 31. a plug-in part; 32. a pressing part; 40. a first seal ring; 50. a gas input; 60. a needle cannula assembly; 61. a positioning block; 610. a body; 611. a flange; 612. a positioning part; 62. a needle tube; 620. a liquid flow channel; 621. a distal end face; 70. a flow-limiting block; 71. a flow restrictor; 72. a base; 720. a bottom end surface; 721. a collision part; 73. a through hole; 80. a second seal ring; 90. a gas flow passage.

Detailed Description

The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

It will be understood that when an element is referred to as being "fixed to" another element, it can be directly on the other element or be present as another intermediate element through which the element is fixed. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. When an element is referred to as being "disposed on" another element, it can be directly on the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like are used herein for illustrative purposes only.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and/or" as used herein includes any and all combinations of one or more of the associated listed items.

As shown in fig. 1 to 6, the bio-ink spraying device of the present invention is used for uniformly spraying high-viscosity bio-ink (cells or medicines).

The bio-ink spray device includes a housing 10, a fluid input assembly 20, a compression block 30, a first seal ring 40, a gas input 50, a needle cannula assembly 60, a restrictor block 70, and a second seal ring 80.

The housing 10 includes a first mounting portion 11, a main body 12, and a second mounting portion 13. The first mounting portion 11 and the second mounting portion 13 are detachably mounted to both ends of the main body 12, respectively. The first mounting portion 11 is provided with internal threads, the body 12 is provided with external threads at both ends, and the second mounting portion 13 is provided with internal threads. The first mounting portion 11 is screw-mounted to an upper end portion of the main body 12, and the second mounting portion 13 is screw-mounted to a lower end portion of the main body 12.

The fluid input assembly 20 includes an input fitting 21 and an input tube 22. The input connection 21 is provided with an external thread and the input pipe 22 extends partly into the input connection 21, the input pipe 22 being provided with a fluid input 221.

The pressing block 30 includes a socket portion 31 and a pressing portion 32, and the socket portion 31 extends from the pressing portion 32. The pressing block 30 is provided with a mounting hole penetrating the insertion portion 31 and the pressing portion 32. The insertion portion 31 and the pressing portion 32 are cylindrical, and the diameter of the insertion portion 31 is larger than that of the pressing portion 32. The end of the pressing portion 32 away from the plugging portion 31 is a plane.

The first seal ring 40 is an annular seal ring.

The gas input member 50 is used for inputting gas into the housing 10.

Needle cannula assembly 60 includes a locating block 61 and a needle cannula 62. The positioning block 61 includes a body 610, a flange 611, and a positioning portion 612. The body 610 is cylindrical and has a hollow structure, and an inner wall of the body 610 is provided with an internal thread. The flange 611 extends from the outer wall of the body 610, has a circular ring shape, and the flange 611 is used for fixing the positioning block 61. The positioning portion 612 extends from the bottom of the body 610. The needle tube 62 has a hollow structure, and a liquid flow passage 620 is formed therein. The end of the needle tube 62 is in interference fit with the positioning part 612, so that the needle tube 62 is fixed on the positioning block 61, and the positioning block 61 and the needle tube 62 form an integrated structure. When the bio-ink spraying device is used for bio-inks of different viscosities, the needle tube assembly 60 can be conveniently replaced integrally, and bio-inks of different viscosities are suitable. The distal end of the needle 62 is provided with a distal face 621. The needle tube 62 has an inner diameter of 0.1 to 0.5mm, an outer diameter of 1.5 to 2 times the inner diameter, and a length of 5 to 30mm.

The restrictor block 70 includes a restrictor 71 and a seat 72. The restrictor 71 extends from the base 72. The flow restriction block 70 is provided with a through hole 73, and the through hole 73 penetrates the flow restriction 71 and the base 72. The distal end of the needle tube 62 extends into the through hole 73, and a gas flow passage 90 is formed between the outer wall of the needle tube 62 and the inner wall of the through hole 73. The needle tube 62 is coaxially matched with the inner hole of the flow limiting block 70, and the coaxiality is between minus 0.05mm and plus 0.05mm; the outer diameter of the needle tube 62 is 0.2-1.0 mm smaller than the inner diameter of the flow limiting block 70; the distal end face 621 of the needle tube 62 does not protrude beyond the bottom end face 720 of the flow block 70, and is limited to-0.5 to 0.5mm in distance from each other. By adjusting the diameter of the through hole 73, the cross-sectional area of the gas flow passage 90 can be changed, and the flow rate of the gas can be changed. The base 72 includes a bottom end surface 720 and an abutting portion 721, and the abutting portion 721 abuts against the second mounting portion 13, so that the flow-restricting block 70 is mounted on the housing 10.

When the bio-ink spraying device is assembled, the compression block 30 is positioned in the positioning block 61 of the needle tube assembly 60, the input tube 22 partially extends into the input connector 21, the input connector 21 is in threaded connection with the positioning block 61, and in the connecting process, the input connector 21 abuts against the compression block 30, so that the input tube 22 is communicated with the needle tube 62 and sealing is achieved. The first seal ring 40 is sleeved on the positioning portion 612 of the positioning block 61. The needle cannula assembly 60 is placed in the body 12, the first mounting portion 11 is threadably coupled to the body 12, and the flange 611 is clamped between the top of the body 12 and the first mounting portion 11 to secure the needle cannula assembly 60 to the housing 10. The gas input 50 is mounted to the body 12. The extending direction of the gas input member 50 is perpendicular to the extending direction of the main body 12. The second sealing ring 80 is sleeved at the tail end of the flow limiting part 71, the flow limiting part 71 of the flow limiting block 70 extends into the main body 12, and a gas flow passage 90 is formed between the outer wall of the needle tube 62 and the inner wall of the through hole 73. The second mounting portion 13 is screw-mounted to the end of the main body 12, so that the flow restriction block 70 is mounted to the housing 10. The flow rate of the bio-ink supplied to the spray device is related to the viscosity of the bio-ink or the inside diameter of the needle 62, and may be 1-20 mL/min. The auxiliary air input flow rate of the spraying device is related to the inner diameter of the flow restriction block 70 or the viscosity coefficient of the bio-ink, and is optionally 1-10L/min. The spraying device, the input flow of the biological ink and the auxiliary air input flow are used for carrying out homogenization spraying on the biological ink with different viscosities by setting the input flow of the biological ink or the auxiliary air input flow parameter of the spraying device, wherein the viscosity coefficient of the sprayed biological ink is preferably 1-300 mPa.s.

When the bio-ink spraying device is used, bio-ink is input from the fluid input port 221, enters the needle tube 62 through the input pipe 22, gas enters from the gas input member 50, enters the gas flow passage 90 through the inside of the main body 12, and the cross section area of the gas flow passage 90 is far smaller than that of the inside of the main body 12The area accelerates the flow rate of the gas, the gas spraying atomizes the bio-ink flowing out of the needle tube 62, the uniform spraying is realized, and the activity and the cell differentiation efficiency after the cell spraying are not affected. The spraying height of the bottom surface of the spraying device from the spraying surface is controlled to be 1-200 mm, preferably 5-100 mm. The spraying device, the input flow of the biological ink and the spraying height are used for controlling the spraying area of the fixed-point spraying by setting the input flow of the biological ink or the spraying height of the spraying device, wherein the spraying area of the fixed-point spraying is preferably 100-1500 mm ² 。

The needle tube assembly 60 is detachably arranged on the shell, and when biological inks with different viscosities are sprayed, the needle tube assembly 60 can be conveniently replaced so as to correspond to the needle tubes 62 with different diameters; the flow limiting block 70 is detachably mounted on the housing 10, and when bio-ink with different viscosities is sprayed, the flow limiting block 70 can be conveniently replaced to adjust the sectional area of the gas flow channel 90, so that the gas spraying speed can be adjusted.

The use of the bio-ink spraying device is described in detail below with different examples:

example 1

In the spraying process of the biological ink, in a certain spraying height range, liquid drops are distributed in a conical shape in space, and the opening angle of the cone is a spraying cone, so that the spraying area is directly influenced. To evaluate the spray area of the spray device, the spray process was photographed with a camera and the spray cone angle of the photographed spray image was calculated in MATLAB software. A single frame image has a low signal-to-noise ratio and inaccurate boundaries as shown in fig. 7 (a). The superimposed image (7 (b)) of a large number of frames significantly improves the image signal-to-noise ratio. The injection angle image shown in 7 (b) is binarized using a Local Adaptive Threshold Segmentation Method (LATSM) to obtain an image in fig. 7 (c). Fitting the boundaries in FIG. 9 using least squares, respectively calculating near field cone angles θ ₁ And far field cone angle theta ₂ (FIG. 7 (d)). The viscosity of the spray objects was measured by using physiological saline, 0.4% (w/v), 0.6% (w/v), 0.8% (w/v) and 1% (w/v) Sodium Alginate (SA) solutions, respectively, and the viscosity of the spray objects was measured by using a viscometer, respectively, as follows:

the inner diameter of the needle tube 62 of the spraying device is set to be 0.2-0.3 mm, the outer diameter is set to be 0.4-0.8 mm, the length is set to be 5-20 mm, the inner diameter of the flow limiting block 70 is set to be 0.8-1.2 mm, and the distance between the tail end surface 621 of the needle tube 62 and the bottom end surface 72 of the flow limiting block 70 is-0.2 mm. Auxiliary air flow rate Q _A Controlling the flow Q of the spraying object at 1-5L/min _L When the constant control is 3mL/min, the theta of different spraying objects ₁ And theta ₂ Measured is shown in fig. 8 and 9, respectively. The spray area of the spot spray of the spray device can be further evaluated by equation (1):

wherein: h is a _N Fitting the height of the position of the straight line breakpoint from the position of the outlet of the spraying device for the least square method, and h _F Is the height of the spray surface from the position of the outlet of the spray device.

The spray object is exemplified by 1% (w/v) SA, when Q of the spray device _L Controlled at 2-4 mL/min, Q _A The spraying states are respectively shown in figures 10 (a), 10 (b), 10 (c) and 10 (d) at 1.0, 1.5, 2.0 and 5.0L/min (the scale is 10 mm); q of the spraying device _A Controlled at 2-4L/min, Q _L The spray areas at different spray heights are shown in FIG. 11 when the spray areas are controlled to be 0.2-4 mL/min, wherein H1, H2, H3 and H4 respectively represent the spray heights of 25, 50, 75 and 100mm. It can be seen that the designed spraying device can realize 100-1200 mm under the proper spraying parameters ² Is arranged on the surface of the spray nozzle.

Example two

To illustrate the spraying performance of the spraying device, 1% (w/v) SA was used as the spraying object, Q _A Controlled at 2-3L/min, Q _L The spraying height is set at 50-100 mm and the spraying states of 100 and 500 mu L are respectively and quantitatively sprayed by adopting a spraying device, wherein the spraying states are respectively shown in fig. 12 (a) and 12 (b), and the scale is 10mm. In the case of figure 12 (c),the viscous solution was dispensed as squeeze droplets using a 1mL syringe needle. Compared with the liquid drops extruded by the injector, the size and the distribution of the liquid drops sprayed by the spraying device are more uniform. When the spraying device is used for spraying the volume of 500 mu L, the liquid drops can uniformly cover a spraying surface with a certain area.

Further, a fixed area of pigskin was fixed on a plane inclined at an angle of 45 °, and 1% (w/v) SA (FIGS. 13 (a) and 13 (b)) and physiological saline (FIGS. 13 (c) and 13 (d)) were sprayed on the pigskin by a spraying device, respectively. It can be seen that the droplets generated from the viscous SA solution are more uniformly maintained on the surface of the pigskin than the physiological saline, which has a very serious runoff problem.

Example III

To further illustrate the spray performance of the spray device on viscous bio-ink, a quantity of human immortalized epidermal cells (HaCaT, china, COBIO-ER) was added to a 1% (w/v) SA solution prepared with physiological saline to prepare a cell density of about 10 ⁵ And taking the biological ink of cells/mL as a spraying object. Q (Q) _A Controlled at 2-3L/min, Q _L The spraying height is controlled to be 2-3 mL/min and is set to be 50-100 mm. The structure of the spraying device is the same as in the first embodiment. After 4 hours and 24 hours of spraying the bio-ink with the spraying device, the sprayed sample was stained with a Calcein AM/PI kit (ref.c2015l, beyotime Biotechnology, china), three-dimensional scanning imaging was performed with a laser confocal microscope, and then 3D images were spliced with Imaris software, and it was seen that cells were three-dimensionally distributed in 4 hours of spraying with 1% (w/v) SA viscous solution as a carrier (fig. 14 (a)), and after 24 hours of spraying, the cells all sunk into the bottom of the container (fig. 14 (b)). It was observed that cell suspensions in physiological saline as a carrier generally settled for a large part within half an hour. This also demonstrates that the spray device can avoid uneven cell spraying caused by sedimentation of cells of a low viscosity carrier in a short time during spray printing or drug delivery.

The foregoing examples illustrate only a few embodiments of the invention, which are described in detail and are not to be construed as limiting the scope of the invention. It should be noted that, for those skilled in the art, it is possible to make several modifications and improvements without departing from the concept of the present invention, which are equivalent to the above embodiments according to the essential technology of the present invention, and these are all included in the protection scope of the present invention.

Claims (10)

1. A bio-ink spraying device, comprising a housing, characterized in that: the bio-ink spraying device further comprises a fluid input assembly, a gas input part, a needle tube assembly and a flow limiting block, wherein an inner hole is formed in the shell, the fluid input assembly, the gas input part and the needle tube assembly are both arranged in the shell, the gas input part is communicated with the inner hole, the needle tube assembly comprises a needle tube, the needle tube is provided with a liquid flow passage, the liquid flow passage is communicated with the fluid input assembly, the flow limiting block is arranged in the shell, the flow limiting block is provided with a flow limiting part, the flow limiting block is provided with a through hole, the through hole penetrates through the flow limiting part, the flow limiting part is arranged in the inner hole, the tail end of the needle tube is arranged in the through hole, a gas flow passage is formed between the outer wall of the needle tube and the inner wall of the through hole, the gas flow passage is communicated with the inner hole, and the sectional area of the gas flow passage is smaller than that the gas input by the gas input part accelerates to uniformly atomize the output liquid.

2. The bio-ink spraying apparatus according to claim 1, wherein: the needle tube assembly is detachably mounted to the housing.

3. The bio-ink spraying apparatus according to claim 2, wherein: the needle tube assembly comprises a positioning block and a needle tube, and the needle tube is fixedly matched with the positioning block.

4. A bio-ink spraying device according to claim 3 wherein: the needle tube is in interference fit with the positioning block.

5. A bio-ink spraying device according to claim 3 wherein: the positioning block comprises a body and a flange extending from the body, the shell comprises a first installation part and a main body, the first installation part is detachably connected with the main body, the positioning block stretches into the main body, and the first installation part is abutted against the flange to enable the positioning block to be detachably installed in the shell.

6. The bio-ink spraying apparatus according to claim 5, wherein: the fluid input assembly comprises an input joint and an input pipe arranged on the input joint, and the input joint stretches into the positioning block and is in threaded connection with the positioning block.

7. The bio-ink spraying apparatus according to claim 6, wherein: the bio-ink spraying device further comprises a compression block, the compression block comprises an inserting connection part and a compression part, the tail end of the input pipe extends into the compression block, the inserting connection part is positioned between the outer wall of the input pipe and the inner wall of the input joint, the compression part is in contact with the input joint and the positioning block, and the compression block enables the input pipe to be in sealing connection with the needle tube.

8. The bio-ink spraying apparatus according to claim 1, wherein: the flow limiting block is detachably arranged on the shell.

9. The bio-ink spraying apparatus according to claim 8, wherein: the shell comprises a main body and a second installation part, the second installation part is detachably installed on the main body, the current limiting block is further provided with a base, the current limiting part extends from the base and comprises a collision part, and the collision part collides with the second installation part to enable the current limiting block to be installed on the shell.

10. The bio-ink spraying apparatus according to claim 9, wherein: the base is provided with a bottom end face, the needle tube is provided with a tail end face, and the second installation part is in threaded connection with the main body so as to adjust the distance between the tail end face and the bottom end face.

Images