CN109053587A - A method of the separation and Extraction tetrahydropyrimidine from halophilic microorganism fermentation liquid - Google Patents
A method of the separation and Extraction tetrahydropyrimidine from halophilic microorganism fermentation liquid Download PDFInfo
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- CN109053587A CN109053587A CN201811010620.8A CN201811010620A CN109053587A CN 109053587 A CN109053587 A CN 109053587A CN 201811010620 A CN201811010620 A CN 201811010620A CN 109053587 A CN109053587 A CN 109053587A
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- tetrahydropyrimidine
- thallus
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- fermentation liquid
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D239/00—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings
- C07D239/02—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings
- C07D239/06—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings having one double bond between ring members or between a ring member and a non-ring member
Abstract
The invention discloses a kind of method of separation and Extraction tetrahydropyrimidine from halophilic microorganism fermentation liquid, this method operating procedure is as follows: obtaining tetrahydropyrimidine crystal after primary centrifugation-immersion swelling-secondary centrifuging-concentration-removing protein thallus-desalination-condensing crystallizing.The invention has the following advantages: 1) extract the tetrahydropyrimidine in halophilic microorganism fermentation liquid with the method that ethyl alcohol is swollen, since ethanol molecule is small, diffusion velocity is fast, substantially increases speed compared with adding pure water to dissolve thallus；2) with reasonable solid-liquid proportion, the tetrahydropyrimidine substance in cell is made to reach maximum release, to obtain the tetrahydropyrimidine substance of high concentration；3) rotary process twice removes sodium chloride, thallus and albumen in fermentation liquid, significantly reduces the processing load of subsequent handling；4) ethyl alcohol after extracting can also recycle again, save resource, achieve the purpose that recycle.
Description
Technical field
The present invention relates to the field of biotechnology for extracting tetrahydropyrimidine, specifically a kind of from halophilic microorganism fermentation liquid
The method of middle separation and Extraction tetrahydropyrimidine.
Background technique
Halophilic microorganism is a kind of bacillus, can be fermented to prepare tetrahydropyrimidine.Tetrahydropyrimidine is this halophilic microorganism
The substance of cell interior is resulted from, protection can be provided to cell, albumen, cell membrane and cell in extreme circumstances, have and protect
Immune system is protected, heat shock protein, the functions such as protection membrane structure are synthesized.In addition, tetrahydropyrimidine has moisturizing to human skin, prevents
The functions such as ultraviolet and anti-aging, can be applied to the fields such as biological medicine, fine chemistry industry.
Obtain tetrahydropyrimidine, it is necessary to be discharged into the tetrahydropyrimidine in somatic cells in solution.In finding separation and Extraction
In the patent document of tetrahydropyrimidine, patent name is in " a kind of extraction process of tetrahydropyrimidine ", and elaborating will be phonetic containing tetrahydro
The crushing thallus of pyridine substance is added deionized water by solid-to-liquid ratio 1:5-15 and carries out dissolution stirring, dissolves out tetrahydropyrimidine substance, so
After stand, remove upper layer floating bacterial chip, obtain the solution of the substance containing tetrahydropyrimidine.The method will be first is that using that will dry bacterium
The mechanical means that body crushes, Grin-ding energ7 is high and is unfavorable for the abundant extraction of intracellular product, second is that being dissolved in water, at subsequent place
During reason, large amount of sewage is generated, not only waste water resource but also unfavorable to environment.Patent name is that " one kind is divided from fermentation liquid
A kind of method for extracting tetrahydropyrimidine using dual membrane system is disclosed in the patent document of method from extraction tetrahydropyrimidine ", this
Although kind of method energy consumption is lower, thallus and albumen are easy to block membrane aperture, substantially reduce the service life of film, are not inconsistent
Close the economy of actual production.Patent name is " method that culture halophilic microorganism produces tetrahydropyrimidine and hydroxy tetrahydro pyrimidine "
Patent in, be only referred to be extracted with ethyl alcohol and discharge intracellular product, but the specific method for being extracted with ethyl alcohol and such as
What releases this intracellular product of tetrahydropyrimidine to the maximum extent has no and is discussed in detail discussion.
Summary of the invention
Technical assignment of the invention is to provide a kind of method of separation and Extraction tetrahydropyrimidine from halophilic microorganism fermentation liquid.
Technical assignment of the invention is realized in the following manner:
A method of the separation and Extraction tetrahydropyrimidine from halophilic microorganism fermentation liquid, this method operating procedure are as follows:
1) primary centrifugation: the halophilic microorganism fermentation liquid that will be enriched in tetrahydropyrimidine is centrifuged in high power centrifuge, obtains thallus；
2) it impregnates swelling: is added into gained thallus with centrifugation fermentating liquid volume than the ethanol solution for 1:0.1-0.3；By bacterium mud
It is sufficiently impregnated in the ethanol solution 3-8 hours, ethanol molecule is made to penetrate into cell interior, cell volume expands, i.e.,
Cell swelling；Cell swells to cell wall rupture, and internal tetrahydropyrimidine is discharged into solution；
3) secondary centrifuging: the solution impregnated after being swollen is centrifuged in high power centrifuge again, then thallus is abandoned, takes supernatant
Liquid；
4) it is concentrated: the supernatant is evaporated, recycle the ethyl alcohol in supernatant, pure water is added into the supernatant of evaporation and concentration
Dilution；
5) removing protein thallus: by the supernatant after the dilution of above-mentioned pure water through ceramic membrane filter, few portion in the supernatant is removed
Divide albumen and thallus residue；
6) it desalination: by except the supernatant soln of deproteinized and thallus residue is through cation and anion exchange resin purification, removes
Salinity in solution；
7) condensing crystallizing: being concentrated into refractive power 75-80% for the solution after desalination, and then ladder is cooled to -4 DEG C of crystallizations, is centrifuged later
Obtain tetrahydropyrimidine crystal.
Centrifuge speed used is once centrifuged in the step 1) at 4000-5000 revs/min；The step 3)
Centrifuge speed used in middle secondary centrifuging is at 4500-4800 revs/min；Centrifugation centrifugal basket used in the step 7)
Speed is at 4500-5000 revs/min.
The mass percent concentration of ethanol solution used is 50-70% in the step 2).
In supernatant after being concentrated by evaporation in the step 4), dilution pure water, pure water and step 1 fermentation liquid is added
Volume ratio is 1:4-6.
Ceramic membrane aperture is 0.04-0.06 microns in the step 5).
Step 6) the middle-jiao yang, function of the spleen and stomach anion exchange resin uses the cation exchange resin of model 001 × 7, model
The anion exchange resin of D301 or D296.
Electrical conductivity of solution after removing salinity in the step 6) is 12-20 μ s/cm.
Concentration is using heating concentration or film condensing mode in the step 7).
A kind of method of separation and Extraction tetrahydropyrimidine from halophilic microorganism fermentation liquid of the invention compared to the prior art,
It has the advantages that
1) tetrahydropyrimidine in halophilic microorganism fermentation liquid is extracted with the method that ethyl alcohol is swollen, since ethanol molecule is small, diffusion speed
Degree is fast, substantially increases speed compared with adding pure water to dissolve thallus；
2) with reasonable solid-liquid proportion, the tetrahydropyrimidine substance in cell is made to reach maximum release, to obtain highly concentrated
The tetrahydropyrimidine substance of degree；
3) rotary process twice removes sodium chloride, thallus and albumen in fermentation liquid, and the processing for significantly reducing subsequent handling is negative
Load；
4) ethyl alcohol after extracting can also recycle again, save resource, achieve the purpose that recycle.
Specific embodiment
Embodiment 1:
1) primary centrifugation: the halophilic microorganism fermentation liquid 6L rich in tetrahydropyrimidine is taken, is centrifuged to obtain thallus 141 in high power centrifuge
Gram, 4500 revs/min of centrifuge speed；
2) it impregnates swelling: the ethanol solution 1200ml of mass percent concentration 60% being added to the thallus, impregnate 4 hours, make ethyl alcohol
Molecule infiltration enters cell interior, cell volume expansion, and the tetrahydropyrimidine of cell wall rupture, inside is discharged into solution；
3) secondary centrifuging: solution being centrifuged in high power centrifuge again, 4600 revs/min of centrifugal rotational speed, and centrifugation thallus is lost
It abandons, obtains supernatant 1185ml；
4) it is concentrated: the supernatant is evaporated, recycle the ethyl alcohol in supernatant, be added into the supernatant of evaporation and concentration
The dilution of 1000ml pure water；
5) removing protein thallus: crossing ceramic membrane for the supernatant, and the aperture of ceramic membrane is 0.05 micron, removes a small amount of albumen and thallus
Residue；
6) solution successively desalination: is crossed to the cation exchange resin of model 001 × 7, the anion exchange tree of model D301
Rouge is refined, 15 μ s/cm of acquired solution conductivity；
7) condensing crystallizing: being concentrated into refractive power 75% for solution, and ladder is cooled to -4 DEG C of crystallizations, later be sent into 4600 turns of centrifugal rotational speed/
Centrifugation obtains 114.5 grams of tetrahydropyrimidine crystal in the high power centrifuge of minute, purity 99.2%.
Embodiment 2:
1) primary centrifugation: the halophilic microorganism fermentation liquid 6L rich in tetrahydropyrimidine is taken, is centrifuged to obtain thallus 123 in high power centrifuge
Gram, 5000 revs/min of centrifuge speed；
2) it impregnates swelling: the ethanol solution 1500ml of mass percent concentration 70% being added to the thallus, impregnate 3 hours, make ethyl alcohol
Molecule infiltration enters cell interior, cell volume expansion, and the tetrahydropyrimidine of cell wall rupture, inside is discharged into solution；
3) secondary centrifuging: solution being centrifuged in high power centrifuge again, 4700 revs/min of centrifugal rotational speed, and centrifugation thallus is lost
It abandons, obtains supernatant 1455ml；
4) it is concentrated: the supernatant is evaporated, recycle the ethyl alcohol in supernatant, be added into the supernatant of evaporation and concentration
The dilution of 1350ml pure water；
5) removing protein thallus: crossing ceramic membrane for the supernatant, and the aperture of ceramic membrane is 0.05 micron, removes a small amount of albumen and thallus
Residue；
6) solution successively desalination: is crossed to the cation exchange resin of model 001 × 7, the anion exchange tree of model D301
Rouge is refined, 19 μ s/cm of acquired solution conductivity；
7) condensing crystallizing: being concentrated into refractive power 78% for solution, and ladder is cooled to -4 DEG C of crystallizations, later be sent into 4700 turns of centrifugal rotational speed/
Centrifugation obtains 117 grams of tetrahydropyrimidine crystal in the high power centrifuge of minute, purity 98.6%.
Embodiment 3:
1) primary centrifugation: taking the halophilic microorganism fermentation liquid 6L rich in tetrahydropyrimidine, 98 grams of thallus be centrifuged to obtain in high power centrifuge,
4500 revs/min of centrifuge speed；
2) it impregnates swelling: the ethanol solution 1000ml of mass percent concentration 50% being added to the thallus, impregnate 8 hours, make ethyl alcohol
Molecule infiltration enters cell interior, cell volume expansion, and the tetrahydropyrimidine of cell wall rupture, inside is discharged into solution；
3) secondary centrifuging: solution being centrifuged in high power centrifuge again, 4600 revs/min of centrifugal rotational speed, and centrifugation thallus is lost
It abandons, obtains supernatant 985ml；
4) it is concentrated: the supernatant is evaporated, recycle the ethyl alcohol in supernatant, be added into the supernatant of evaporation and concentration
The dilution of 1100ml pure water；
5) removing protein thallus: crossing ceramic membrane for the supernatant, and the aperture of ceramic membrane is 0.05 micron, removes a small amount of albumen and thallus
Residue；
6) solution successively desalination: is crossed to the cation exchange resin of model 001 × 7, the anion exchange tree of model D296
Rouge is refined, 13 μ s/cm of acquired solution conductivity；
7) condensing crystallizing: being concentrated into refractive power 75% for solution, and ladder is cooled to -4 DEG C of crystallizations, later be sent into 4900 turns of centrifugal rotational speed/
Centrifugation obtains 102 grams of tetrahydropyrimidine crystal in the high power centrifuge of minute, purity 98.9%.
The technical personnel in the technical field can readily realize the present invention with the above specific embodiments,.But it answers
Work as understanding, the present invention is not limited to above-mentioned several specific embodiments.On the basis of the disclosed embodiments, the technology
The technical staff in field can arbitrarily combine different technical features, to realize different technical solutions.
Claims (8)
1. a kind of method of the separation and Extraction tetrahydropyrimidine from halophilic microorganism fermentation liquid, which is characterized in that this method operation step
It is rapid as follows:
1) primary centrifugation: the halophilic microorganism fermentation liquid that will be enriched in tetrahydropyrimidine is centrifuged in high power centrifuge, obtains thallus；
2) it impregnates swelling: is added into gained thallus with centrifugation fermentating liquid volume than the ethanol solution for 1:0.1-0.3；By bacterium mud
It is sufficiently impregnated in the ethanol solution 3-8 hours, ethanol molecule is made to penetrate into cell interior, cell volume expands, i.e.,
Cell swelling；Cell swells to cell wall rupture, and internal tetrahydropyrimidine is discharged into solution；
3) secondary centrifuging: the solution impregnated after being swollen is centrifuged in high power centrifuge again, then thallus is abandoned, takes supernatant
Liquid；
4) it is concentrated: the supernatant is evaporated, recycle the ethyl alcohol in supernatant, pure water is added into the supernatant of evaporation and concentration
Dilution；
5) removing protein thallus: by the supernatant after the dilution of above-mentioned pure water through ceramic membrane filter, few portion in the supernatant is removed
Divide albumen and thallus residue；
6) it desalination: by except the supernatant soln of deproteinized and thallus residue is through cation and anion exchange resin purification, removes
Salinity in solution；
7) condensing crystallizing: being concentrated into refractive power 75-80% for the solution after desalination, and then ladder is cooled to -4 DEG C of crystallizations, is centrifuged later
Obtain tetrahydropyrimidine crystal.
2. the method according to claim 1 for extracting tetrahydropyrimidine, which is characterized in that be once centrifuged in the step 1)
Centrifuge speed used is at 4000-5000 revs/min；Centrifuge speed used in secondary centrifuging exists in the step 3)
4500-4800 revs/min；Centrifugation centrifuge speed used is at 4500-5000 revs/min in the step 7).
3. the method according to claim 1 for extracting tetrahydropyrimidine, which is characterized in that ethyl alcohol used in the step 2)
The mass percent concentration of solution is 50-70%.
4. the method according to claim 1 for extracting tetrahydropyrimidine, which is characterized in that be concentrated by evaporation in the step 4)
In supernatant afterwards, dilution pure water is added, the volume ratio of pure water and step 1 fermentation liquid is 1:4-6.
5. the method according to claim 1 for extracting tetrahydropyrimidine, which is characterized in that ceramic fenestra in the step 5)
Diameter is 0.04-0.06 microns.
6. the method according to claim 1 for extracting tetrahydropyrimidine, which is characterized in that step 6) the middle-jiao yang, function of the spleen and stomach anion
Exchanger resin uses the cation exchange resin of model 001 × 7, the anion exchange resin of model D301 or D296.
7. the method according to claim 1 for extracting tetrahydropyrimidine, which is characterized in that remove salinity in the step 6)
Electrical conductivity of solution afterwards is 12-20 μ s/cm.
8. the method according to claim 1 for extracting tetrahydropyrimidine, which is characterized in that concentration uses in the step 7)
Heating concentration or film condensing mode.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111303309A (en) * | 2020-02-19 | 2020-06-19 | 王森林 | Microbial polysaccharide extraction element |
| CN113604415A (en) * | 2021-09-01 | 2021-11-05 | 珠海瑞德林生物有限公司 | Application of recombinant bacillus subtilis and method for producing tetrahydropyrimidine by using wastewater generated in enzymatic synthesis of N-acetylneuraminic acid |
| CN113621549A (en) * | 2021-09-01 | 2021-11-09 | 珠海瑞德林生物有限公司 | Application of recombinant bacillus subtilis and method for producing tetrahydropyrimidine by using waste water generated in enzymatic synthesis of nicotinamide mononucleotide |
| CN114958939A (en) * | 2022-06-02 | 2022-08-30 | 河北常山凯络尼特生物技术有限公司 | Method for reducing elkedox conductivity |
| CN115073381A (en) * | 2022-06-08 | 2022-09-20 | 伊明泰(山东)生物科技有限公司 | Aqueous phase crystallization method in ectoine extraction process |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111303309A (en) * | 2020-02-19 | 2020-06-19 | 王森林 | Microbial polysaccharide extraction element |
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| CN113604415A (en) * | 2021-09-01 | 2021-11-05 | 珠海瑞德林生物有限公司 | Application of recombinant bacillus subtilis and method for producing tetrahydropyrimidine by using wastewater generated in enzymatic synthesis of N-acetylneuraminic acid |
| CN113621549A (en) * | 2021-09-01 | 2021-11-09 | 珠海瑞德林生物有限公司 | Application of recombinant bacillus subtilis and method for producing tetrahydropyrimidine by using waste water generated in enzymatic synthesis of nicotinamide mononucleotide |
| CN114958939A (en) * | 2022-06-02 | 2022-08-30 | 河北常山凯络尼特生物技术有限公司 | Method for reducing elkedox conductivity |
| CN114958939B (en) * | 2022-06-02 | 2024-03-29 | 河北常山凯络尼特生物技术有限公司 | Method for reducing conductivity of ethandibulin |
| CN115073381A (en) * | 2022-06-08 | 2022-09-20 | 伊明泰(山东)生物科技有限公司 | Aqueous phase crystallization method in ectoine extraction process |
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