CN109046485A - A kind of extraction element of drop inclusion - Google Patents
A kind of extraction element of drop inclusion Download PDFInfo
- Publication number
- CN109046485A CN109046485A CN201811063704.8A CN201811063704A CN109046485A CN 109046485 A CN109046485 A CN 109046485A CN 201811063704 A CN201811063704 A CN 201811063704A CN 109046485 A CN109046485 A CN 109046485A
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- Prior art keywords
- cavity
- ellipsoid
- drop
- microchannel
- diameter
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Links
- 238000000605 extraction Methods 0.000 title claims abstract description 31
- 238000002347 injection Methods 0.000 claims abstract description 26
- 239000007924 injection Substances 0.000 claims abstract description 26
- 239000007788 liquid Substances 0.000 claims abstract description 18
- 238000002156 mixing Methods 0.000 claims abstract description 16
- 210000005239 tubule Anatomy 0.000 claims abstract description 6
- 239000000463 material Substances 0.000 claims description 11
- 230000005540 biological transmission Effects 0.000 claims description 5
- 238000000034 method Methods 0.000 abstract description 7
- 238000010586 diagram Methods 0.000 description 4
- 125000000118 dimethyl group Chemical group [H]C([H])([H])* 0.000 description 3
- 239000004205 dimethyl polysiloxane Substances 0.000 description 3
- 235000013870 dimethyl polysiloxane Nutrition 0.000 description 3
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 description 3
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 3
- 229920005573 silicon-containing polymer Polymers 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 238000001125 extrusion Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 230000001746 atrial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000005056 cell body Anatomy 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
Abstract
The present invention relates to a kind of extraction elements of drop inclusion, belong to microfluidic art.The extraction element of the drop inclusion, it is symmetrical inside apparatus body to be distributed with ellipsoid cavity, ellipsoid cavity long axis is placed along the vertical direction, it is connected in the middle part of two spheroid shape inside cavities by microchannel, gas injection is connected to by tubule on the outside of two ellipsoid cavity and detaches device, chamber inlet channel and bottom, which are equipped with, at the top of an ellipsoid cavity in two ellipsoid cavity is equipped with cavity outlet channel, another spheroid shape cavity bottom is equipped with chamber inlet channel and top and is equipped with cavity outlet channel, continuous phase discrete phase mixing liquid enters ellipsoid cavity by cavity access road, after the completion of extraction process, continuous phase discrete phase mixing liquid is flowed out by cavity exit passageway.This extraction element is researched and developed in order to which microflow control technique to be preferably applied for practical, is mainly used for realizing the extraction of the inclusion in drop.
Description
Technical field
The present invention relates to a kind of extraction elements of drop inclusion, belong to microfluidic art.
Background technique
The development of microflow control technique is so that drop becomes common drug, cell, a glue in biomedical, macro work
The carrier of body particle, reactant, and the extraction process of these inclusions mostly uses the physical method of contact at present, often makes
At the breakage or death of inclusion.It is badly in need of developing extraction of the new contactless method of one kind to realize inclusion.
Using bubble as power, squeezing drop since bubble can deform not will cause drop strenuous exercise, will not
There is too much influence to inclusion therein.Meanwhile it also being be unable to do without microflow control technique, raw materials used apparatus of the present invention are exactly continuous
The microfluid that phase discrete phase mixing liquid is constituted, the manipulation to fluid is the key that Success in Experiment.
Summary of the invention
For the above-mentioned problems of the prior art and deficiency, the present invention provides a kind of extraction element of drop inclusion.
This extraction element is researched and developed in order to which microflow control technique to be preferably applied for practical, is mainly used for realizing the inclusion in drop
Extraction.The invention is realized by the following technical scheme.
A kind of extraction element of drop inclusion, including chamber inlet channel 1, ellipsoid cavity 2, gas injection with detach
Device 3, cavity outlet channel 4, apparatus body 5 and microchannel 6,5 inside of apparatus body is symmetrical to be distributed with ellipsoid cavity
2,2 long axis of ellipsoid cavity is placed along the vertical direction, and two 2 middle insides of ellipsoid cavity are connected to by microchannel 6, and two ellipse
2 outside of ball-type cavity is connected to gas injection by tubule and detaches device 3, a spheroid shape in two ellipsoid cavity 2
It is equipped with chamber inlet channel 1 and bottom at the top of cavity 2 and is equipped with cavity outlet channel 4, another 2 bottom of ellipsoid cavity is equipped with
Chamber inlet channel 1 and top are equipped with cavity outlet channel 4, and continuous phase discrete phase mixing liquid passes through cavity access road 1
Into ellipsoid cavity 2, after the completion of extraction process, continuous phase discrete phase mixing liquid passes through cavity exit passageway 4 and flows out.
The chamber inlet channel 1 and 4 diameter of cavity outlet channel are identical as the diameter that a discrete phase drips, and diameter is desirable
2~3mm。
The short axle of the ellipsoid cavity 2 is 3 ~ 5 times of diameter of discrete phase drop, can use 6 ~ 15mm.
6 two sides of microchannel are trumpet type entrance, and bell-mouthed diameter is equal to discrete phase and drips diameter, can use 2 ~ 3mm；It is micro-
6 narrowest diameter of channel is 3 ~ 5 times of extract diameter, can use 30 ~ 50 μm.
The chamber inlet channel 1 and 4 vertical position of cavity outlet channel are corresponding, chamber inlet channel 1 and cavity outlet
4 equal length of channel.
The gas injection with to detach device 3 corresponding with 6 horizontal position of microchannel.
The ellipsoid cavity 2, cavity outlet channel 4 and 6 material of microchannel are transparent and light transmission material.
Above-mentioned continuous phase discrete phase mixing liquid is continuous phase packet discrete phase, is not discrete phase packet continuous phase.Continuous phase stream
Body and discrete phase drop are immiscible, and color has apparent difference, and continuous phase and the volume ratio of discrete phase are certain, such as continuously
Phase: discrete phase volume ratio=3:2.
Above-mentioned gas injection is to be able to achieve gas injection and detach the existing of function with the device and structure detached in device 3
Structure, such as the structure disclosed in number of patent application CN201310310609.4.
The extraction element working principle of the drop inclusion are as follows:
As shown in Fig. 2, one group of continuous phase discrete phase mixing liquid from the chamber inlet channel 1 of 2 upper end of left side ellipsoid cavity into
Enter left side ellipsoid cavity 2, meanwhile, another group of continuous phase discrete phase mixing liquid from the access road 1 of right side cavity lower end into
Enter right side ellipsoid cavity 2.When left side includes the discrete phase drop of discrete phase drop and right side not comprising extract of extract
Reach ellipsoid cavity 2 appropriate location when, the gas injection of two sides and detach device 3 by control system control start to work.
When just starting, two gas injections are inflated simultaneously with gas injection in device 3 is detached, and keep the nozzle end of gas injection apparatus raw
At bubble.Continue to inflate, bubble is grown up therewith.When air bubble expansion to a certain extent when, will push loudspeaker of the drop to microchannel 6
Type entrance is mobile, until drop is arrived on trumpet type entrance.Due to wall effect effect and horn-type structure the characteristics of,
Drop will be fixed on trumpet type inlet.At this point, left gas injection device continues to inflate, Top right gas injection device is slowly taken out
Gas.Left side drop is by by the continuous extrusion because of air bubble expansion, and due to the presence of Surface Tension of Liquid Drops, drop will not break scattered, liquid
Extract inside drop will be mobile to microchannel 6 by continuous extrusion, and final extract can run out from drop internal, and entrance is micro-
In channel 6.Because of the pumping of Top right gas injection device, right atrial pressure will reduce in microchannel 6, under the action of pressure difference, mention
Object is taken to will be moved at the drop of right side.It is evacuated Top right gas injection device persistently, left gas injection device is persistently inflated, when
When pressure difference reaches a certain level, extract will enter in the drop of right side.At this moment, the gas injection apparatus of two sides is all evacuated, and makes gas
Bubble disappears, and the extract of left side drop is transferred into right side drop.Left side drop will be flowed out from the cavity outlet channel of lower end,
Right side includes that the drop of extract will be flowed out from the cavity outlet channel of upper end.
The beneficial effects of the present invention are:
1, apparatus of the present invention realize the extraction of inclusion using contactless method, can avoid the breakage or dirt of inclusion
Dye.
2, apparatus of the present invention time-consuming is short, and consumptive material is few, at low cost and high-efficient.
3, apparatus of the present invention not only can be used to extract various solid particle inclusions, such as colloidal solid etc. can also be used
To extract cell body isoreactivity substance.
Detailed description of the invention
Fig. 1 is schematic structural view of the invention；
Fig. 2 is apparatus of the present invention working principle diagram；
Fig. 3 is apparatus of the present invention droplet capture schematic diagram；
Fig. 4 is apparatus of the present invention particle migration schematic diagram；
Fig. 5 is apparatus of the present invention drop release schematic diagram.
In figure: 1- chamber inlet channel, 2- ellipsoid cavity and detach device at 3- gas injection, 4- cavity outlet channel,
5- apparatus body, the microchannel 6-.
Specific embodiment
With reference to the accompanying drawings and detailed description, the invention will be further described.
Embodiment 1
As shown in Fig. 1 and 3 to 5, the extraction element of the drop inclusion, including chamber inlet channel 1, ellipsoid cavity 2, gas
Device 3, cavity outlet channel 4, apparatus body 5 and microchannel 6 are injected and detach, 5 inside of apparatus body is symmetrical to be distributed with
Ellipsoid cavity 2,2 long axis of ellipsoid cavity are placed along the vertical direction, and two 2 middle insides of ellipsoid cavity pass through microchannel 6
Connection, two 2 outsides of ellipsoid cavity are connected to gas injection by tubule and detach device 3, in two ellipsoid cavity 2
It is equipped with chamber inlet channel 1 and bottom at the top of one ellipsoid cavity 2 and is equipped with cavity outlet channel 4, another spheroid shape chamber
2 bottom of body is equipped with chamber inlet channel 1 and top and is equipped with cavity outlet channel 4, continuous phase discrete phase mixing liquid transit chamber
Body access road 1 enters ellipsoid cavity 2, and after the completion of extraction process, continuous phase discrete phase mixing liquid is logical by cavity outlet
It flows out in road 4.
Wherein chamber inlet channel 1 and 4 diameter of cavity outlet channel are identical as the diameter that a discrete phase drips, and diameter is desirable
2mm；The short axle of the ellipsoid cavity 2 is 3 ~ 5 times of diameter of discrete phase drop, can use 6mm；6 two sides of microchannel are loudspeaker
Type entrance, bell-mouthed diameter are equal to discrete phase and drip diameter, can use 2mm；6 narrowest diameter of microchannel be extract diameter 3 ~
5 times, it can use 30 μm；The chamber inlet channel 1 and 4 vertical position of cavity outlet channel are corresponding, chamber inlet channel 1 and cavity
4 equal length of exit passageway；The gas injection with to detach device 3 corresponding with 6 horizontal position of microchannel；The ellipsoid cavity
2, cavity outlet channel 4 and 6 material of microchannel are transparent and light transmission PDMS(dimethyl silicone polymer) material.
Embodiment 2
As shown in Fig. 1 and 3 to 5, the extraction element of the drop inclusion, including chamber inlet channel 1, ellipsoid cavity 2, gas
Device 3, cavity outlet channel 4, apparatus body 5 and microchannel 6 are injected and detach, 5 inside of apparatus body is symmetrical to be distributed with
Ellipsoid cavity 2,2 long axis of ellipsoid cavity are placed along the vertical direction, and two 2 middle insides of ellipsoid cavity pass through microchannel 6
Connection, two 2 outsides of ellipsoid cavity are connected to gas injection by tubule and detach device 3, in two ellipsoid cavity 2
It is equipped with chamber inlet channel 1 and bottom at the top of one ellipsoid cavity 2 and is equipped with cavity outlet channel 4, another spheroid shape chamber
2 bottom of body is equipped with chamber inlet channel 1 and top and is equipped with cavity outlet channel 4, continuous phase discrete phase mixing liquid transit chamber
Body access road 1 enters ellipsoid cavity 2, and after the completion of extraction process, continuous phase discrete phase mixing liquid is logical by cavity outlet
It flows out in road 4.
Wherein chamber inlet channel 1 and 4 diameter of cavity outlet channel are identical as the diameter that a discrete phase drips, and diameter is desirable
3mm；The short axle of the ellipsoid cavity 2 is 3 ~ 5 times of diameter of discrete phase drop, can use 15mm；6 two sides of microchannel are loudspeaker
Type entrance, bell-mouthed diameter are equal to discrete phase and drip diameter, can use 3mm；6 narrowest diameter of microchannel be extract diameter 3 ~
5 times, it can use 50 μm；The chamber inlet channel 1 and 4 vertical position of cavity outlet channel are corresponding, chamber inlet channel 1 and cavity
4 equal length of exit passageway；The gas injection with to detach device 3 corresponding with 6 horizontal position of microchannel；The ellipsoid cavity
2, cavity outlet channel 4 and 6 material of microchannel are transparent and light transmission PDMS(dimethyl silicone polymer) material.
Embodiment 3
As shown in Fig. 1 and 3 to 5, the extraction element of the drop inclusion, including chamber inlet channel 1, ellipsoid cavity 2, gas
Device 3, cavity outlet channel 4, apparatus body 5 and microchannel 6 are injected and detach, 5 inside of apparatus body is symmetrical to be distributed with
Ellipsoid cavity 2,2 long axis of ellipsoid cavity are placed along the vertical direction, and two 2 middle insides of ellipsoid cavity pass through microchannel 6
Connection, two 2 outsides of ellipsoid cavity are connected to gas injection by tubule and detach device 3, in two ellipsoid cavity 2
It is equipped with chamber inlet channel 1 and bottom at the top of one ellipsoid cavity 2 and is equipped with cavity outlet channel 4, another spheroid shape chamber
2 bottom of body is equipped with chamber inlet channel 1 and top and is equipped with cavity outlet channel 4, continuous phase discrete phase mixing liquid transit chamber
Body access road 1 enters ellipsoid cavity 2, and after the completion of extraction process, continuous phase discrete phase mixing liquid is logical by cavity outlet
It flows out in road 4.
Wherein chamber inlet channel 1 and 4 diameter of cavity outlet channel are identical as the diameter that a discrete phase drips, and diameter is desirable
3mm；The short axle of the ellipsoid cavity 2 is 3 ~ 5 times of diameter of discrete phase drop, can use 10mm；6 two sides of microchannel are loudspeaker
Type entrance, bell-mouthed diameter are equal to discrete phase and drip diameter, can use 3mm；6 narrowest diameter of microchannel be extract diameter 3 ~
5 times, it can use 40 μm；The chamber inlet channel 1 and 4 vertical position of cavity outlet channel are corresponding, chamber inlet channel 1 and cavity
4 equal length of exit passageway；The gas injection with to detach device 3 corresponding with 6 horizontal position of microchannel；The ellipsoid cavity
2, cavity outlet channel 4 and 6 material of microchannel are saturating transparent and light transmission PDMS(dimethyl silicone polymer) material.
In conjunction with attached drawing, the embodiment of the present invention is explained in detail above, but the present invention is not limited to above-mentioned
Embodiment within the knowledge of a person skilled in the art can also be before not departing from present inventive concept
Put that various changes can be made.
Claims (7)
1. a kind of extraction element of drop inclusion, it is characterised in that: including chamber inlet channel (1), ellipsoid cavity (2),
Gas injection and device (3), cavity outlet channel (4), apparatus body (5) and microchannel (6) are detached, in left inside apparatus body (5)
Right symmetrical equipped with ellipsoid cavity (2), ellipsoid cavity (2) long axis is placed along the vertical direction, two ellipsoid cavity (2)
Middle inside is connected to by microchannel (6), is connected to gas injection by tubule on the outside of two ellipsoid cavity (2) and is detached dress
It sets (3), is equipped with chamber inlet channel (1) at the top of an ellipsoid cavity (2) in two ellipsoid cavity (2) and bottom is set
There are cavity outlet channel (4), another ellipsoid cavity (2) bottom is equipped with chamber inlet channel (1) and top is equipped with cavity
Exit passageway (4), continuous phase discrete phase mixing liquid enter ellipsoid cavity (2) by cavity access road (1), extraction process
After the completion, continuous phase discrete phase mixing liquid is flowed out by cavity exit passageway (4).
2. the extraction element of drop inclusion according to claim 1, it is characterised in that: the chamber inlet channel (1)
It is identical as the diameter that a discrete phase drips with cavity outlet channel (4) diameter.
3. the extraction element of drop inclusion according to claim 1, it is characterised in that: the ellipsoid cavity (2)
Short axle is 3 ~ 5 times of diameter of discrete phase drop.
4. the extraction element of drop inclusion according to claim 1, it is characterised in that: microchannel (6) two sides are
Trumpet type entrance, bell-mouthed diameter are equal to discrete phase and drip diameter；Microchannel (6) narrowest diameter is the 3 ~ 5 of extract diameter
Times.
5. the extraction element of drop inclusion according to claim 1, it is characterised in that: the chamber inlet channel (1)
It is corresponding with cavity outlet channel (4) vertical position, chamber inlet channel (1) and cavity outlet channel (4) equal length.
6. the extraction element of drop inclusion according to claim 1, it is characterised in that: the gas injection and detach dress
It is corresponding with microchannel (6) horizontal position to set (3).
7. according to claim 1 to the extraction element of drop inclusion described in 6 any one, it is characterised in that: the ellipsoid
Cavity (2), cavity outlet channel (4) and microchannel (6) material are transparent and light transmission material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811063704.8A CN109046485A (en) | 2018-09-12 | 2018-09-12 | A kind of extraction element of drop inclusion |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811063704.8A CN109046485A (en) | 2018-09-12 | 2018-09-12 | A kind of extraction element of drop inclusion |
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| Publication Number | Publication Date |
|---|---|
| CN109046485A true CN109046485A (en) | 2018-12-21 |
Family
ID=64761534
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811063704.8A Pending CN109046485A (en) | 2018-09-12 | 2018-09-12 | A kind of extraction element of drop inclusion |
Country Status (1)
| Country | Link |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110059556A1 (en) * | 2009-09-04 | 2011-03-10 | The Research Foundation Of State University Of New York | Rapid and Continuous Analyte Processing in Droplet Microfluidic Devices |
| WO2012135259A1 (en) * | 2011-03-30 | 2012-10-04 | Gnubio, Inc. | Injection of multiple volumes into or out of droplets |
| US20160136643A1 (en) * | 2013-03-15 | 2016-05-19 | Lariat Biosciences, Inc. | Microfluidic methods for manipulating dna |
| US20170354937A1 (en) * | 2009-06-26 | 2017-12-14 | President And Fellows Of Harvard College | Fluid injection |
| CN209109193U (en) * | 2018-09-12 | 2019-07-16 | 昆明理工大学 | A kind of extraction element of drop inclusion |
-
2018
- 2018-09-12 CN CN201811063704.8A patent/CN109046485A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20170354937A1 (en) * | 2009-06-26 | 2017-12-14 | President And Fellows Of Harvard College | Fluid injection |
| US20110059556A1 (en) * | 2009-09-04 | 2011-03-10 | The Research Foundation Of State University Of New York | Rapid and Continuous Analyte Processing in Droplet Microfluidic Devices |
| WO2012135259A1 (en) * | 2011-03-30 | 2012-10-04 | Gnubio, Inc. | Injection of multiple volumes into or out of droplets |
| US20160136643A1 (en) * | 2013-03-15 | 2016-05-19 | Lariat Biosciences, Inc. | Microfluidic methods for manipulating dna |
| CN209109193U (en) * | 2018-09-12 | 2019-07-16 | 昆明理工大学 | A kind of extraction element of drop inclusion |
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