CN109043544A - A kind of preparation method of the yeast selenium oligopeptides powder rich in B family vitamin - Google Patents
A kind of preparation method of the yeast selenium oligopeptides powder rich in B family vitamin Download PDFInfo
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- CN109043544A CN109043544A CN201810738787.XA CN201810738787A CN109043544A CN 109043544 A CN109043544 A CN 109043544A CN 201810738787 A CN201810738787 A CN 201810738787A CN 109043544 A CN109043544 A CN 109043544A
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- 239000011669 selenium Substances 0.000 title claims abstract description 62
- 240000004808 Saccharomyces cerevisiae Species 0.000 title claims abstract description 58
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 title claims abstract description 55
- 229910052711 selenium Inorganic materials 0.000 title claims abstract description 55
- 102000040350 B family Human genes 0.000 title claims abstract description 36
- 108091072128 B family Proteins 0.000 title claims abstract description 36
- 239000011782 vitamin Substances 0.000 title claims abstract description 36
- 235000013343 vitamin Nutrition 0.000 title claims abstract description 36
- 229940088594 vitamin Drugs 0.000 title claims abstract description 36
- 229930003231 vitamin Natural products 0.000 title claims abstract description 36
- 150000003722 vitamin derivatives Chemical class 0.000 title claims abstract description 36
- 108010038807 Oligopeptides Proteins 0.000 title claims abstract description 26
- 102000015636 Oligopeptides Human genes 0.000 title claims abstract description 26
- 239000000843 powder Substances 0.000 title claims abstract description 25
- 238000002360 preparation method Methods 0.000 title claims abstract description 20
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 61
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims abstract description 56
- 235000019441 ethanol Nutrition 0.000 claims abstract description 40
- 239000007864 aqueous solution Substances 0.000 claims abstract description 24
- 230000010355 oscillation Effects 0.000 claims abstract description 22
- 239000008055 phosphate buffer solution Substances 0.000 claims abstract description 22
- 239000000463 material Substances 0.000 claims abstract description 21
- 239000004365 Protease Substances 0.000 claims abstract description 14
- 238000000034 method Methods 0.000 claims abstract description 13
- 230000008569 process Effects 0.000 claims abstract description 9
- 239000002994 raw material Substances 0.000 claims abstract description 9
- 108090000526 Papain Proteins 0.000 claims abstract description 8
- 101000693530 Staphylococcus aureus Staphylokinase Proteins 0.000 claims abstract description 8
- 238000009777 vacuum freeze-drying Methods 0.000 claims abstract description 8
- 108091005804 Peptidases Proteins 0.000 claims abstract description 7
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 7
- 239000003513 alkali Substances 0.000 claims abstract description 7
- 229940055729 papain Drugs 0.000 claims abstract description 7
- 235000019834 papain Nutrition 0.000 claims abstract description 7
- 235000019419 proteases Nutrition 0.000 claims abstract description 7
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 21
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 17
- 238000006243 chemical reaction Methods 0.000 claims description 15
- HEMHJVSKTPXQMS-UHFFFAOYSA-M sodium hydroxide Inorganic materials [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 14
- 239000000654 additive Substances 0.000 claims description 13
- 230000000996 additive effect Effects 0.000 claims description 13
- 239000000243 solution Substances 0.000 claims description 13
- 229910000397 disodium phosphate Inorganic materials 0.000 claims description 9
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 claims description 9
- 238000004140 cleaning Methods 0.000 claims description 8
- 238000007710 freezing Methods 0.000 claims description 8
- 230000008014 freezing Effects 0.000 claims description 8
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 8
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 7
- 230000003472 neutralizing effect Effects 0.000 claims description 7
- 238000002791 soaking Methods 0.000 claims description 7
- 239000007836 KH2PO4 Substances 0.000 claims description 4
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 4
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 4
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 4
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 4
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 claims description 3
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 3
- 229910052698 phosphorus Inorganic materials 0.000 claims description 3
- 239000011574 phosphorus Substances 0.000 claims description 3
- 230000004888 barrier function Effects 0.000 claims description 2
- 239000012531 culture fluid Substances 0.000 claims description 2
- 229910052739 hydrogen Inorganic materials 0.000 claims description 2
- 239000001257 hydrogen Substances 0.000 claims description 2
- 239000011259 mixed solution Substances 0.000 claims description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
- 239000006227 byproduct Substances 0.000 claims 1
- KCIDZIIHRGYJAE-YGFYJFDDSA-L dipotassium;[(2r,3r,4s,5r,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] phosphate Chemical compound [K+].[K+].OC[C@H]1O[C@H](OP([O-])([O-])=O)[C@H](O)[C@@H](O)[C@H]1O KCIDZIIHRGYJAE-YGFYJFDDSA-L 0.000 claims 1
- 125000004435 hydrogen atom Chemical class [H]* 0.000 claims 1
- 239000000047 product Substances 0.000 claims 1
- 239000011734 sodium Substances 0.000 claims 1
- 229910052708 sodium Inorganic materials 0.000 claims 1
- 229910000162 sodium phosphate Inorganic materials 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 abstract description 8
- 108090000623 proteins and genes Proteins 0.000 abstract description 8
- 108090000765 processed proteins & peptides Proteins 0.000 abstract description 6
- 102000004196 processed proteins & peptides Human genes 0.000 abstract description 5
- 150000001413 amino acids Chemical class 0.000 abstract description 4
- 235000013305 food Nutrition 0.000 abstract description 4
- 230000001502 supplementing effect Effects 0.000 abstract description 3
- 235000011649 selenium Nutrition 0.000 description 44
- 238000010438 heat treatment Methods 0.000 description 10
- 235000018102 proteins Nutrition 0.000 description 7
- 102000008114 Selenoproteins Human genes 0.000 description 6
- 108010074686 Selenoproteins Proteins 0.000 description 6
- 230000000052 comparative effect Effects 0.000 description 5
- 241000235342 Saccharomycetes Species 0.000 description 4
- 235000001014 amino acid Nutrition 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 150000002978 peroxides Chemical class 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- FDKWRPBBCBCIGA-REOHCLBHSA-N (2r)-2-azaniumyl-3-$l^{1}-selanylpropanoate Chemical compound [Se]C[C@H](N)C(O)=O FDKWRPBBCBCIGA-REOHCLBHSA-N 0.000 description 1
- FDKWRPBBCBCIGA-UHFFFAOYSA-N 2-azaniumyl-3-$l^{1}-selanylpropanoate Chemical compound [Se]CC(N)C(O)=O FDKWRPBBCBCIGA-UHFFFAOYSA-N 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 208000002177 Cataract Diseases 0.000 description 1
- FDKWRPBBCBCIGA-UWTATZPHSA-N D-Selenocysteine Natural products [Se]C[C@@H](N)C(O)=O FDKWRPBBCBCIGA-UWTATZPHSA-N 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 108010058643 Fungal Proteins Proteins 0.000 description 1
- 108010053070 Glutathione Disulfide Proteins 0.000 description 1
- 102000006587 Glutathione peroxidase Human genes 0.000 description 1
- 108700016172 Glutathione peroxidases Proteins 0.000 description 1
- 102000004157 Hydrolases Human genes 0.000 description 1
- 108090000604 Hydrolases Proteins 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 102000016943 Muramidase Human genes 0.000 description 1
- 108010014251 Muramidase Proteins 0.000 description 1
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 1
- 208000016222 Pancreatic disease Diseases 0.000 description 1
- RJFAYQIBOAGBLC-BYPYZUCNSA-N Selenium-L-methionine Chemical compound C[Se]CC[C@H](N)C(O)=O RJFAYQIBOAGBLC-BYPYZUCNSA-N 0.000 description 1
- RJFAYQIBOAGBLC-UHFFFAOYSA-N Selenomethionine Natural products C[Se]CCC(N)C(O)=O RJFAYQIBOAGBLC-UHFFFAOYSA-N 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 210000002390 cell membrane structure Anatomy 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- -1 disodium hydrogen Chemical class 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 229960003180 glutathione Drugs 0.000 description 1
- YPZRWBKMTBYPTK-BJDJZHNGSA-N glutathione disulfide Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@H](C(=O)NCC(O)=O)CSSC[C@@H](C(=O)NCC(O)=O)NC(=O)CC[C@H](N)C(O)=O YPZRWBKMTBYPTK-BJDJZHNGSA-N 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 150000002440 hydroxy compounds Chemical class 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 208000019423 liver disease Diseases 0.000 description 1
- 229960000274 lysozyme Drugs 0.000 description 1
- 239000004325 lysozyme Substances 0.000 description 1
- 235000010335 lysozyme Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 210000004994 reproductive system Anatomy 0.000 description 1
- 125000003748 selenium group Chemical group *[Se]* 0.000 description 1
- ZKZBPNGNEQAJSX-UHFFFAOYSA-N selenocysteine Natural products [SeH]CC(N)C(O)=O ZKZBPNGNEQAJSX-UHFFFAOYSA-N 0.000 description 1
- 235000016491 selenocysteine Nutrition 0.000 description 1
- 229940055619 selenocysteine Drugs 0.000 description 1
- 229960002718 selenomethionine Drugs 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 230000009469 supplementation Effects 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 239000011691 vitamin B1 Substances 0.000 description 1
- 239000011716 vitamin B2 Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/18—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from yeasts
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/15—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/16—Inorganic salts, minerals or trace elements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The preparation method of the invention discloses a kind of yeast selenium oligopeptides powder rich in B family vitamin, step is: the A, preparation of raw material yeast rich in selenium；B, yeast rich in selenium is added in alcohol hydrochloric acid aqueous solution and is impregnated, whole process increases supersonic oscillations and vacuum cavitations；C, continue supersonic oscillations, break negative pressure, lye is added and neutralizes；D, cryogenic vacuum concentration abjection part ethyl alcohol and moisture content；E, it is mixed into phosphate buffer solution, addition alkali protease, pH is adjusted, adds papain and neutral proteinase；F, it is put into frozen storehouse control temperature；H, vacuum and low temperature is concentrated, and vacuum freeze drying is to get a kind of yeast selenium oligopeptides powder rich in B family vitamin.The method of the present invention is simple and easy to do, the yeast selenium oligopeptides powder rich in B family vitamin obtained, the molecular weight of protein peptides is 500-700 dalton (Da), 3-5 amino acid, it is easy to absorb, organic selenium content 900-1800ppm, B family vitamin content are the excellent food and raw-food material for supplementing selenium element and B family vitamin in 2000-4000ppm.
Description
Technical field
The invention belongs to food processing technology fields, are more particularly to a kind of yeast selenium oligopeptides powder rich in B family vitamin
Preparation method.
Background technique
Selenium organism the main synthesis that bio-tissue albumen is participated in selenocysteine and selenomethionine, into
And complete physiological metabolism activity, human body has found that 25 kinds of selenoproteins are that important physiological metabolism is indispensable, prevention with
It plays an important role in terms of the diseases such as treating cancer, cardiovascular disease, hepatopathy, cataract, pancreatic disease, diabetes, reproductive system.It is special
It is not the activated centre glutathione peroxidase (GSH-Px) is selenocystein, selenium is the constituent of GSH-Px enzyme system,
It, which can be catalyzed GSH, becomes GSSG, and toxic peroxide is made to be reduced into nontoxic hydroxy compounds, to protect the knot of cell membrane
Structure and function be not by the interference of peroxide and damage.
Saccharomycete almost accounts for the half content of yeast dry matter because main component is protein, therefore turns as biology
Change the main carriers of organic selenium, Se content alreadys exceed 2000ppm in process of production, is the excellent choosing of mankind's selenium-supply.Together
When yeast essential amino acid content it is sufficient, the lysine content of relatively shortage is more especially in cereal.On the other hand, contain
A large amount of vitamin B1, vitamin B2And niacin etc..So comprehensive battalion of the Se-enriched yeast compared with other selenium-rich raw materials as selenium-supply
It is with the obvious advantage to support element.
Currently, spray drying is mainly taken in the processing to Se-enriched yeast, temperature is higher, serious to the destruction of B family vitamin.
Meanwhile the macromoleculars such as unresolved selenoprotein are not easy the problem of absorbing.
Summary of the invention
In view of the deficiencies in the prior art, the purpose of the invention is to provide a kind of yeast seleniums rich in B family vitamin
The preparation method of oligopeptides powder.This method is easy to operation, the yeast selenium oligopeptides powder rich in B family vitamin of acquisition, protein peptides
Molecular weight be 500-700 dalton (Da), 3-5 amino acid is easy to absorb, and organic selenium content 900-1800ppm, B race ties up
Raw cellulose content is the excellent food and raw-food material for supplementing selenium element and B family vitamin in 2000-4000ppm.
In order to achieve the above purpose, the present invention uses following technical measures:
Yeast selenium oligopeptides powder and unremitting spy of the inventor by a large number of experiments using Se-enriched yeast preparation rich in B family vitamin
Rope is finally obtained following technical solution:
A kind of preparation method of the yeast selenium oligopeptides powder rich in B family vitamin, includes the following steps:
(1) it using the self-produced Se-enriched yeast bacteria culture fluid of fermentor as raw material, is inhaled and is considered by microporous barrier vacuum, clean 3-6 times extremely
Completely；
(2) yeast rich in selenium for cleaning up step (1), is added in alcohol hydrochloric acid aqueous solution and impregnates 30-90min,
Entire soaking process is placed in supersonic oscillations and subnormal ambient carries out；
(3) continue supersonic oscillations, break negative pressure, lye is added and neutralizes；
(4) abjection part ethyl alcohol and moisture content is concentrated in low-temperature vacuum environment in the material after neutralizing step (3)；Wherein, very
Reciprocal of duty cycle control are as follows: -0.6 °~-1 °, temperature are as follows: 40-65 DEG C, ethyl alcohol and the total removal amount of moisture content reach 20-50%；
(5) phosphate buffer solution is added in step (4) resulting material, adjusting pH is 3.5-6, then adds Papain
Enzyme and neutral proteinase, additive amount are respectively 0.5wt ‰, 0.7wt ‰, react 1-3h at being then 45-55 DEG C in temperature；
(6) phosphate buffer solution is added in step (5) resulting material, adjusting pH is 9-11, adds alkali protease, adds
Dosage is 0.5 ‰ -0.7 ‰, reacts 1-3h at being then 55-65 DEG C in temperature；
(7) after reaction, products therefrom is put into frozen storehouse and controls temperature -18~-40 DEG C freezing icing, processing is about
12-36 hours；
(8) products therefrom is concentrated in vacuum and low temperature after the completion of step (7) are handled, vacuum freeze drying, vacuum degree control
- 0.8 °~-1.0 ° are made as, 40-65 DEG C of material surface temperature, material center temperature is not higher than -12 DEG C, handles time 5-18h, i.e.,
Obtain a kind of yeast selenium oligopeptides powder rich in B family vitamin.
Preferably, the alcohol hydrochloric acid aqueous solution in the step (2) are as follows: the ethanol water 500- of 10-35vol%
Dissolve in 1-5 parts of HCl in 2000 parts, the mass ratio of yeast rich in selenium and alcohol hydrochloric acid aqueous solution is 1:1.2~1:2.
Preferably, the supersonic oscillations power in the step (2) is 120-170W/m2, time 12-30min is described
Negative pressure is -0.6 ° to -1 °, at interval of 5-15 minutes plus a negative pressure, is maintained negative pressure 5-15 minutes.
Preferably, the lye in the step (3) is the potassium hydroxide of 0.1mol/L or the sodium hydroxide of 0.1mol/L
Solution.
Preferably, the phosphate buffer solution in the step (5) is sodium dihydrogen phosphate (NaH2PO4) and disodium hydrogen phosphate
(Na2HPO4) or potassium dihydrogen phosphate (KH2PO4) and dipotassium hydrogen phosphate (K2HPO4) or potassium dihydrogen phosphate (KH2PO4) and phosphorus
Sour disodium hydrogen (Na2HPO4) or sodium dihydrogen phosphate (NaH2PO4) and dipotassium hydrogen phosphate (K2HPO4) mixed solution is one such
Combination.
The yeast selenium oligopeptides powder rich in B family vitamin obtained by above-mentioned measure, the molecular weight of protein peptides are 500-700
Dalton (Da), 3-5 amino acid are easy to absorb, and organic selenium content 900-1800ppm, B family vitamin content is in 2000-
4000ppm.It is the excellent food and raw-food material for supplementing selenium element and B family vitamin.
Compared with prior art, the invention has the benefit that
(1) preparation method of the present invention as a whole, complements each other, and mainly solves selenoprotein and is not easy to absorb, adds
B family vitamin is lost the technical problem underlyings such as serious during work；Wherein, in step (2) under negative pressure adjusting, by Se-enriched yeast
Bacterium is ultrasonically treated in the environment of alcohol hydrochloric acid aqueous solution, has both destroyed the cell membrane of saccharomycete, accelerates the dissolution of protein,
The higher structure for efficiently destroying protein, helps to improve enzymatic hydrolysis selenoprotein efficiency in later step, while also protecting
Other nutritional ingredients in saccharomycete including B family vitamin are protected, step (5), (6) are that hydrolysis selenoprotein obtains water solubility
The committed step of albumen selects hydrolase type, by buffer solution strict control system pH, reaction temperature and time, obtains
The oligopeptides of amino acid count issue is obtained, step (8) is rich in the important drying in B family vitamin yeast selenium oligopeptides powder preparation process
Step, Vacuum Freezing & Drying Technology ensure principle active component in sample from destroying.
(2) present invention breaks yeast cells film by negative pressure ultrasound portion under the conditions of acidic ethanol, increases the molten of yeast protein
Out and the self-dissolving of lysozyme, yeast selenium protein peptides are obtained by two step enzyme methods, during processing strict temperature control, protected
Its B family vitamin abundant is protected.Yeast rich in selenium is made full use of, more now to the list of saccharomycete or yeast rich in selenium
The separation of one nutrient utilizes, and the synthesis for belonging to various nutrient elements makes full use of scope, it is contemplated that is easily absorbed by the human body etc. and to ask
Topic, it is rarely seen in report.Yeast selenium protein peptides during edible are used in conjunction with B family vitamin is comprehensive, either in effect,
Or all relatively abundant in terms of nutritional supplementation, a variety of effect collaborations.
Specific embodiment
The following is specific embodiments of the present invention, and technical scheme of the present invention is further described, but of the invention
Protection scope be not limited to these examples.It is all to be included in this hair without departing substantially from the change of present inventive concept or equivalent substitute
Within bright protection scope.
Embodiment 1:
A kind of preparation method of the yeast selenium oligopeptides powder rich in B family vitamin, includes the following steps:
A, it is the raw material of culture solution with yeast rich in selenium (Angel Yeast Co., Ltd in purchase Yichang), passes through micropore
Film vacuum, which is inhaled, considers, and cleans up；
B, the yeast rich in selenium for cleaning up step (1) is added in alcohol hydrochloric acid aqueous solution and impregnates, and impregnates 50min, whole
A soaking process is placed in supersonic oscillations and subnormal ambient carries out；Wherein, alcohol hydrochloric acid aqueous solution are as follows: the ethyl alcohol of 20% (vol)
Dissolve in 3 parts of HCl in 900 parts of aqueous solution, the mass ratio of yeast and alcohol hydrochloric acid aqueous solution is 1:1.6.The supersonic oscillations
Power control is in 150W/m2.Vacuum cavitations, at interval of 10 minutes plus a negative pressure, maintain negative pressure 11 minutes at -0.8 °；
C, continue supersonic oscillations, break negative pressure, it is 7 that the potassium hydroxide that 0.1mol/L is added, which is neutralized to pH,；
D, abjection part ethyl alcohol and moisture content is concentrated in low-temperature vacuum environment in the material after neutralizing step (3)；Wherein, the step
Vacuum and low temperature concentration in rapid: vacuum degree control are as follows: -0.8 °, heating temperature are as follows: 56 DEG C, ethyl alcohol reaches with the total removal amount of moisture
40%.
E, be mixed into phosphate buffer solution, add papain and neutral proteinase, respectively additive amount be 0.5 ‰,
0.7 ‰, 53 DEG C of temperature are controlled, 2.5h is reacted.
Phosphate buffer solution in this described step is sodium dihydrogen phosphate (NaH2PO4) and disodium hydrogen phosphate
(Na2HPO4),
F, it is mixed into phosphate buffer solution, adjusting pH is 9, adds alkali protease, and additive amount is 0.6 ‰, controls temperature
60 DEG C, after reacting 2h, it is 7 that 0.1mol hydrochloric acid solution, which is added, and is neutralized to pH.
H, after reaction, be put into frozen storehouse control temperature: -40 DEG C of freezings freeze, and the processing time is for 24 hours.
G, after the reaction was completed vacuum and low temperature concentration (vacuum degree control are as follows: -0.9 °, heating temperature are as follows: 60 DEG C, handle the time
2.5h), vacuum freeze drying, vacuum degree control are as follows: -0.9 °, material surface temperature: 54 DEG C, material center temperature is not higher than -14
DEG C, time 14h is handled to get a kind of yeast selenium oligopeptides powder rich in B family vitamin.
Embodiment 2
A kind of preparation method of the yeast selenium oligopeptides powder rich in B family vitamin, includes the following steps:
A, it is the raw material of culture solution with yeast rich in selenium (Angel Yeast Co., Ltd in purchase Yichang), passes through micropore
Film vacuum, which is inhaled, considers, and cleans up；
B, the yeast rich in selenium for cleaning up step (1) is added in alcohol hydrochloric acid aqueous solution and impregnates, and impregnates 60min, whole
A soaking process is placed in supersonic oscillations and subnormal ambient carries out；Wherein, alcohol hydrochloric acid aqueous solution are as follows: the ethyl alcohol of 20% (vol)
Dissolve in 3 parts of HCl in 900 parts of aqueous solution, the mass ratio of yeast and alcohol hydrochloric acid aqueous solution is 1:1.8.The supersonic oscillations
Power control is in 150W/m2.Vacuum cavitations, at interval of 10 minutes plus a negative pressure, maintain negative pressure 11 minutes at -0.8 °；
C, continue supersonic oscillations, break negative pressure, it is 7 that the potassium hydroxide that 0.1mol/L is added, which is neutralized to pH,；
D, abjection part ethyl alcohol and moisture content is concentrated in low-temperature vacuum environment in the material after neutralizing step (3)；Wherein, the step
Vacuum and low temperature concentration in rapid: vacuum degree control are as follows: -0.9 °, heating temperature are as follows: 56 DEG C, ethyl alcohol reaches with the total removal amount of moisture
35%.
E, be mixed into phosphate buffer solution, add papain and neutral proteinase, respectively additive amount be 0.5 ‰,
0.7 ‰, 53 DEG C of temperature are controlled, 2.5h is reacted.
Phosphate buffer solution in this described step is sodium dihydrogen phosphate (NaH2PO4) and disodium hydrogen phosphate
(Na2HPO4),
F, it is mixed into phosphate buffer solution, adjusting pH is 9, adds alkali protease, and additive amount is 0.6 ‰, controls temperature
60 DEG C, after reacting 2h, it is 7 that 0.1mol hydrochloric acid solution, which is added, and is neutralized to pH.
H, after reaction, be put into frozen storehouse control temperature: -40 DEG C of freezings freeze, and the processing time is 30h.
G, after the reaction was completed vacuum and low temperature concentration (vacuum degree control are as follows: -0.9 °, heating temperature are as follows: 60 DEG C, handle the time
2.5h), vacuum freeze drying, vacuum degree control are as follows: -0.9 °, material surface temperature: 54 DEG C, material center temperature is not higher than -14
DEG C, time 12h is handled to get a kind of yeast selenium oligopeptides powder rich in B family vitamin.
Comparative example 1:
A kind of preparation method of the yeast selenium oligopeptides powder rich in B family vitamin, includes the following steps:
A, it is the raw material of culture solution with yeast rich in selenium (Angel Yeast Co., Ltd in purchase Yichang), passes through micropore
Film vacuum, which is inhaled, considers, and cleans up；
B, the yeast rich in selenium for cleaning up step (1) is added in the ethanol water of 20% (vol) and impregnates, and impregnates
50min, entire soaking process is placed in supersonic oscillations and subnormal ambient carries out；Wherein, the mass ratio of yeast and ethanol water
For 1:1.6.The supersonic oscillations power control is in 150W/m2.Vacuum cavitations are at interval of 10 minutes plus primary at -0.8 °
Negative pressure maintains negative pressure 11 minutes；
C, continue supersonic oscillations, break negative pressure, it is 7 that the potassium hydroxide that 0.1mol/L is added, which is neutralized to pH,；
D, abjection part ethyl alcohol and moisture content is concentrated in low-temperature vacuum environment in the material after neutralizing step (3)；Wherein, the step
Vacuum and low temperature concentration in rapid: vacuum degree control are as follows: -0.8 °, heating temperature are as follows: 56 DEG C, ethyl alcohol reaches with the total removal amount of moisture
40%.
E, be mixed into phosphate buffer solution, add papain and neutral proteinase, respectively additive amount be 0.5 ‰,
0.7 ‰, 53 DEG C of temperature are controlled, 2.5h is reacted.
Phosphate buffer solution in this described step is sodium dihydrogen phosphate (NaH2PO4) and disodium hydrogen phosphate
(Na2HPO4),
F, it is mixed into phosphate buffer solution, adjusting pH is 9, adds alkali protease, and additive amount is 0.6 ‰, controls temperature
60 DEG C, after reacting 2h, it is 7 that 0.1mol hydrochloric acid solution, which is added, and is neutralized to pH.
H, after reaction, be put into frozen storehouse control temperature: -40 DEG C of freezings freeze, and the processing time is for 24 hours.
G, after the reaction was completed vacuum and low temperature concentration (vacuum degree control are as follows: -0.9 °, heating temperature are as follows: 60 DEG C, handle the time
2.5h), vacuum freeze drying, vacuum degree control are as follows: -0.9 °, material surface temperature: 54 DEG C, material center temperature is not higher than -14
DEG C, time 14h is handled to get a kind of yeast selenium oligopeptides powder rich in B family vitamin.
Comparative example 2:
A kind of preparation method of the yeast selenium oligopeptides powder rich in B family vitamin, includes the following steps:
A, it is the raw material of culture solution with yeast rich in selenium (Angel Yeast Co., Ltd in purchase Yichang), passes through micropore
Film vacuum, which is inhaled, considers, and cleans up；
B, the yeast rich in selenium for cleaning up step (1) is added in alcohol hydrochloric acid aqueous solution and impregnates, and impregnates 50min, whole
A soaking process is placed in supersonic oscillations and subnormal ambient carries out；Wherein, alcohol hydrochloric acid aqueous solution are as follows: the ethyl alcohol of 20% (vol)
Dissolve in 3 parts of HCl in 900 parts of aqueous solution, the mass ratio of yeast and alcohol hydrochloric acid aqueous solution is 1:1.6.The supersonic oscillations
Power control is in 150W/m2.Under an atmospheric pressure, added primary pressure at interval of 10 minutes, maintain pressure 11 minutes；
C, continue supersonic oscillations, break negative pressure, it is 7 that the potassium hydroxide that 0.1mol/L is added, which is neutralized to pH,；
D, abjection part ethyl alcohol and moisture content is concentrated in low-temperature vacuum environment in the material after neutralizing step (3)；Wherein, the step
Vacuum and low temperature concentration in rapid: vacuum degree control are as follows: -0.8 °, heating temperature are as follows: 56 DEG C, ethyl alcohol reaches with the total removal amount of moisture
40%.
E, be mixed into phosphate buffer solution, add papain and neutral proteinase, respectively additive amount be 0.5 ‰,
0.7 ‰, 53 DEG C of temperature are controlled, 2.5h is reacted.
Phosphate buffer solution in this described step is sodium dihydrogen phosphate (NaH2PO4) and disodium hydrogen phosphate
(Na2HPO4),
F, it is mixed into phosphate buffer solution, adjusting pH is 9, adds alkali protease, and additive amount is 0.6 ‰, controls temperature
60 DEG C, after reacting 2h, it is 7 that 0.1mol hydrochloric acid solution, which is added, and is neutralized to pH.
H, after reaction, be put into frozen storehouse control temperature: -40 DEG C of freezings freeze, and the processing time is for 24 hours.
G, after the reaction was completed vacuum and low temperature concentration (vacuum degree control are as follows: -0.9 °, heating temperature are as follows: 60 DEG C, handle the time
2.5h), vacuum freeze drying, vacuum degree control are as follows: -0.9 °, material surface temperature: 54 DEG C, material center temperature is not higher than -14
DEG C, time 14h is handled to get a kind of yeast selenium oligopeptides powder rich in B family vitamin.
Comparative example 3:
A kind of preparation method of the yeast selenium oligopeptides powder rich in B family vitamin, includes the following steps:
A, it is the raw material of culture solution with yeast rich in selenium (Angel Yeast Co., Ltd in purchase Yichang), passes through micropore
Film vacuum, which is inhaled, considers, and cleans up；
B, the yeast rich in selenium for cleaning up step (1) is added in alcohol hydrochloric acid aqueous solution and impregnates, and impregnates 50min, whole
A soaking process is placed in supersonic oscillations and subnormal ambient carries out；Wherein, alcohol hydrochloric acid aqueous solution are as follows: the ethyl alcohol of 20% (vol)
Dissolve in 3 parts of HCl in 900 parts of aqueous solution, the mass ratio of yeast and alcohol hydrochloric acid aqueous solution is 1:1.6.The supersonic oscillations
Power control is in 150W/m2.Vacuum cavitations, at interval of 10 minutes plus a negative pressure, maintain negative pressure 11 minutes at -0.8 °；
C, continue supersonic oscillations, break negative pressure, it is 7 that the potassium hydroxide that 0.1mol/L is added, which is neutralized to pH,；
D, abjection part ethyl alcohol and moisture content is concentrated in low-temperature vacuum environment in the material after neutralizing step (3)；Wherein, the step
Vacuum and low temperature concentration in rapid: vacuum degree control are as follows: -0.8 °, heating temperature are as follows: 56 DEG C, ethyl alcohol reaches with the total removal amount of moisture
40%.
E, be mixed into phosphate buffer solution, add papain and neutral proteinase, respectively additive amount be 0.5 ‰,
0.7 ‰, 53 DEG C of temperature are controlled, 2.5h is reacted.
Phosphate buffer solution in this described step is sodium dihydrogen phosphate (NaH2PO4) and disodium hydrogen phosphate
(Na2HPO4),
F, it is mixed into phosphate buffer solution, adjusting pH is 8 (trypsase peak optimization reaction pH), adds trypsase, respectively
Additive amount is 0.6 ‰, is controlled 37 DEG C of temperature (trypsase peak optimization reaction temperature), and after reacting 2h, 0.1mol hydrochloric acid solution is added
Being neutralized to pH is 7.
H, after reaction, be put into frozen storehouse control temperature: -40 DEG C of freezings freeze, and the processing time is for 24 hours.
G, after the reaction was completed vacuum and low temperature concentration (vacuum degree control are as follows: -0.9 °, heating temperature are as follows: 60 DEG C, handle the time
2.5h), vacuum freeze drying, vacuum degree control are as follows: -0.9 °, material surface temperature: 54 DEG C, material center temperature is not higher than -14
DEG C, time 14h is handled to get a kind of yeast selenium oligopeptides powder rich in B family vitamin.
Product prepared by above-described embodiment and comparative example is measured, as a result as follows:
As can be seen from the above table, the average molecular weight of selenoprotein peptide is proper in 1-2 of embodiment of the present invention products obtained therefrom,
Organic selenium content and B family vitamin total amount are also higher, are superior to comparative example products obtained therefrom.
Simply to illustrate that technical concepts and features of the invention, its purpose is allows in the art above-described embodiment
Those of ordinary skill cans understand the content of the present invention and implement it accordingly, and it is not intended to limit the scope of the present invention.It is all
It is changes or modifications equivalent made by the essence of content according to the present invention, should be covered by the scope of protection of the present invention.
Claims (5)
1. a kind of preparation method of the yeast selenium oligopeptides powder rich in B family vitamin, which comprises the steps of:
(1) it using the self-produced Se-enriched yeast bacteria culture fluid of fermentor as raw material, is inhaled and is considered by microporous barrier vacuum, cleaning 3-6 times to clean；
(2) yeast rich in selenium for cleaning up step (1), is added in alcohol hydrochloric acid aqueous solution and impregnates 30-90min, entirely
Soaking process is placed in supersonic oscillations and subnormal ambient carries out；
(3) continue supersonic oscillations, break negative pressure, lye is added and neutralizes；
(4) abjection part ethyl alcohol and moisture content is concentrated in low-temperature vacuum environment in the material after neutralizing step (3)；Wherein, vacuum degree
Control are as follows: -0.6 °~-1 °, temperature are as follows: 40-65 DEG C, ethyl alcohol and the total removal amount of moisture content reach 20-50%；
(5) be added phosphate buffer solution in step (4) resulting material, adjusting pH is 3.5-6, then add papain and
Neutral proteinase, additive amount are respectively 0.5wt ‰, 0.7wt ‰, react 1-3h at being then 45-55 DEG C in temperature；
(6) phosphate buffer solution is added in step (5) resulting material, adjusting pH is 9-11, adds alkali protease, additive amount
It is 0.5 ‰ -0.7 ‰, reacts 1-3h at being then 55-65 DEG C in temperature；
(7) after reaction, products therefrom is put into frozen storehouse and controls temperature -18~-40 DEG C freezing icing, handle about 12-36
Hour；
(8) step (7) handle after the completion of by products therefrom in vacuum and low temperature be concentrated, vacuum freeze drying, vacuum degree control be-
0.8 °~-1.0 °, 40-65 DEG C of material surface temperature, material center temperature is not higher than -12 DEG C, handles time 5-18h to get one
Kind is rich in the yeast selenium oligopeptides powder of B family vitamin.
2. preparation method according to claim 1, which is characterized in that the alcohol hydrochloric acid aqueous solution in the step (2)
Are as follows: 1-5 parts of HCl, yeast rich in selenium and alcohol hydrochloric acid aqueous solution are dissolved in 500-2000 parts of ethanol water of 10-35vol%
Mass ratio be 1:1.2~1:2.
3. -2 described in any item preparation methods according to claim 1, which is characterized in that the ultrasonic wave vibration in the step (2)
Swinging power is 120-170W/m2, time 12-30min, the negative pressure is -0.6 ° to -1 °, at interval of 5-15 minutes plus primary
Negative pressure maintains negative pressure 5-15 minutes.
4. preparation method according to claim 1-3, which is characterized in that the lye in the step (3) is
The potassium hydroxide of 0.1mol/L or the sodium hydroxide solution of 0.1mol/L.
5. preparation method according to claim 1, which is characterized in that the phosphate buffer solution in the step (5) is phosphorus
Acid dihydride sodium (NaH2PO4) and disodium hydrogen phosphate (Na2HPO4) or potassium dihydrogen phosphate (KH2PO4) and dipotassium hydrogen phosphate
(K2HPO4) or potassium dihydrogen phosphate (KH2PO4) and disodium hydrogen phosphate (Na2HPO4) or sodium dihydrogen phosphate (NaH2PO4) and phosphorus
Sour hydrogen dipotassium (K2HPO4) the one such combination of mixed solution.
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