CN109043130A - A kind of liquid man-made feeds and preparation method thereof of the new small peaceful mite of Pasteur - Google Patents
A kind of liquid man-made feeds and preparation method thereof of the new small peaceful mite of Pasteur Download PDFInfo
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- CN109043130A CN109043130A CN201811014967.XA CN201811014967A CN109043130A CN 109043130 A CN109043130 A CN 109043130A CN 201811014967 A CN201811014967 A CN 201811014967A CN 109043130 A CN109043130 A CN 109043130A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
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Abstract
The invention discloses a kind of liquid man-made feeds and preparation method thereof of the new small peaceful mite of Pasteur.Fluid present invention man-made feeds formula mainly includes rice moth egg, Decapsulated Artemia Cysts, sucrose, honey, peptone, yeast extract, Fresh Egg Huang and water.Its preparation method is simple, and raw material have been commercialized, and source is wide and is easy to get.Man-made feeds provided by the invention can satisfy the new small peaceful mite nutritional need of Pasteur, and feeding effect is preferable, can be used for the large-scale breeding of the new small peaceful mite of Pasteur.
Description
Technical field
The invention belongs to natural enemy Predatory Mites to raise field, and in particular to a kind of Pasteur's newly small peaceful mite man-made feeds and its preparation
Method.
Background technique
For a long time, since the abuse of chemical pesticide and misuse lead to many small-sized suction juice harmful organism such as tetranychids, Ji
The Rampant damage in various crop such as horse, aleyrodid, aphid, chemical prevention are also more and more difficult.Compare therewith, Predatory Mites are but because of it
Small, development is fastly, breeding amount is big, prey ability is strong, occupies the characteristics such as similar ecological niche to harmful organism, can be to above-mentioned several classes
Pest harmful mite effectively plays control action (Xu Xuenong, 2013).
The new small peaceful mite of PasteurNeoseiulus barkeri, belong to Acari Acari, post mite catalogue Parasitiforme,
Gamasid suborder Gamasida, Phytoseiidae Phytoseiidae, small NeosciulusNeoseiulus.Belong to polyphagy Predatory Mites kind
Class, natural food have tetranychid, tarsonemid, flour mite, tiny insect, pollen and fungi etc. (Bakker and Sabelis,
1989；Fan and Petitt, 1994a；Fan and Petitt, 1994b; Hessein and Parrella,1991;
Momen and Abdelkhader,2010;Wu et al, 2016).Wide with foraging territories, developmental rate is fast, natural death
The features such as rate is low, and egg laying amount is high, and diffusivity is strong, it is considered to be one of best biological and ecological methods to prevent plant disease, pests, and erosion natural enemy (Bonde, 1989), it is extensive
Biological control applied to the small-sized juice absorption pest such as tetranychid, thrips.
In the biological control using Predatory Mites, how to obtain for field adaptability requirement, be to restrict Predatory Mites
The key factor (Zhang Baoxin etc., 2007) of large-area applications.Therefore, a kind of method for breeding being simple and efficient is mass propagation predation
The prerequisite (Lenteren, 2003) of mite.Currently, the propagation method of Predatory Mites has nature prey breeding method, substitution prey numerous
Grow method, pollen breeding method etc. (Zhang Baoxin etc., 2007).It is new small peaceful that Pasteur can be raised on a small scale with natural prey such as Tetranychus urticae
Mite, during commercial scale metaplasia produces, usually using tyrophagus putrescentiae, ellipse dispersion etc. as substitution prey raising
(Li et al, 2016).When being raised with above-mentioned prey, it is big that there are occupied spaces, and Predatory Mites and prey are difficult to separate counting, catch
The problems such as food mite harvest time is difficult to control.In addition, Predatory Mites are raised with substitution prey flour mite, to direct labor's unsoundness risk,
Sometimes resulted in during release human allergy (Bolckmans and Van Houten, 2006; Fernandez-Caldas
Et al, 2007).Predatory Mites are bred using man-made feeds, it is unfavorable that the breeding brings such as nature prey, substitution prey can be eliminated
It influences (Riddick, 2009).
Summary of the invention
Primary and foremost purpose of the invention is to provide that a kind of raw material sources are wide, and at low cost, preparation method is simple, is suitable for extensive
Raise the liquid man-made feeds and preparation method thereof of the new small peaceful mite of Pasteur.
A kind of new small peaceful mite liquid man-made feeds of Pasteur, formula includes basic ingredient and adding ingredient two major classes substance.
By weight percentage, basic ingredient accounts for 74-76% in the artificial feed formula, and adding ingredient accounts for 24-26%.Base
Plinth is at the mixture for being grouped into sucrose, honey, peptone, yeast extract, Fresh Egg Huang and water.Adding ingredient is by fresh rice
Moth ovum and Decapsulated Artemia Cysts composition.
Basic ingredient includes the raw material of following mass ratioes: sucrose 5%, honey 5%, peptone in man-made feeds made of described
5%, yeast extract 5%, Fresh Egg Huang 10%, water 70%.
Adding ingredient includes the raw material of following mass ratioes: 80% rice moth egg, 20% decladding halogen in man-made feeds made of described
Worm's ovum.
Newly small peaceful mite provides Major Nutrient substance for Pasteur for above-mentioned adding ingredient rice moth egg and Decapsulated Artemia Cysts: including albumen
Matter, lipid, lipoprotein, cholesterol, vitamin and mineral element etc..
Peptone and yeast extract are two kinds of high-quality proteins in above-mentioned basic ingredient, and main component is amino acid, nucleosides
Acid, small peptide etc. can regard the complementary element of man-made feeds protein sources.
Sucrose and honey are carbohydrate in above-mentioned basic ingredient, are the main sources of Predatory Mites energy, are Predatory Mites
Glucide necessary to growth and development provides.Meanwhile sucrose is also the important feeding excitor substance of Predatory Mites.
Fresh Egg Huang provides lipid and protein for man-made feeds in above-mentioned basic ingredient.
The present invention also provides a kind of preparation methods of the new small peaceful mite man-made feeds of Pasteur, comprising the following steps:
1) fresh rice moth egg is chosen into impurity, is ground into homogenate；By Decapsulated Artemia Cysts mortar grinder, artemia egg powder is obtained；Newly
Fresh hen egg decladding collects yolk and is placed in beaker that seal up preservative film spare；
2) score weighs each component in mass ratio, and is uniformly mixed；
3) mixture for obtaining step 3 is centrifuged, and supernatant is taken to be packed into centrifuge tube；
4) centrifuge tube for being loaded into man-made feeds is stored in -20 DEG C of refrigerators for use.
Natural enemy mite class of the present invention can be the new small peaceful mite of Pasteur.
Man-made feeds formula of the invention and its technology of preparing are suitable for the new small peaceful mite difference mite state of Pasteur, including young mite,
The raising of deutonymph, adult mite and egg-laying season adult mite.
The advantages of the present invention
(1) the artificial feed formula raw material of the present invention has been commercialized, and raw material sources economic and reliable is at low cost.
(2) the artificial feed formula technology of preparing is simple, easy to operate, is suitable for extensive raising natural enemy mite class.
(3) man-made feeds being prepared can at -20 DEG C long-term preservation, it is easy to use.
The present invention solves the Predatory Mites artificial diet techniques such as the new small peaceful mite of Pasteur there are occupied spaces that big, process is more, catches
The problems such as food mite harvest time is difficult to control.
Man-made feeds of the present invention can normally complete growth and development, lay eggs, incubate for raising the new small peaceful mite of Pasteur
Change,
Beneficial effect is significant.
Specific embodiment
Feature of the invention is described in conjunction with following instance, the given examples are served only to explain the present invention, is not intended to limit
The scope of the present invention.
Embodiment 1
The man-made feeds of the present embodiment, by the material composition of following weight:
Silkworm pupa hemolymph 12g, sucrose 2.4g, honey 2.4g, peptone 2.4g, yeast extract 2.4g, fresh yolk 4.8g
With water 33.6mL.
Preparation method:
Fresh silkworm chrysalis is clean with clear water repeated flushing, then silkworm chrysalis is taken out, placement 10min(is prevented black in 60 DEG C of warm water
Change) after be put into culture dish with 75% alcohol disinfecting, then rinsed with clear water, then use filter paper suck dry moisture, cut off pupa tail portion, receipts
It is spare that collection pupa hemolymph is placed in -20 DEG C of refrigerators；Fresh Egg decladding, collection yolk are placed on spare in beaker；According to embodiment
In each substance weight, weigh each component in the balance and be uniformly mixed；Uniformly mixed solution is centrifuged, supernatant is taken to can be obtained
Liquid man-made feeds.It is spare that man-made feeds loading centrifuge tube is stored in -20 DEG C of refrigerators.
Embodiment 2
The man-made feeds of the present embodiment, by the material composition of following weight:
Silkworm pupa hemolymph 12.8g, Decapsulated Artemia Cysts 3.2g, sucrose 2.4g, honey 2.4g, peptone 2.4g, yeast extract
2.4g, fresh yolk 4.8g and water 33.6mL.
Preparation method:
Fresh silkworm chrysalis is clean with clear water repeated flushing, then silkworm chrysalis is taken out, placement 10min(is prevented black in 60 DEG C of warm water
Change) after be put into culture dish with 75% alcohol disinfecting, then rinsed with clear water, then use filter paper suck dry moisture, cut off pupa tail portion, receipts
It is spare that collection pupa hemolymph is placed in -20 DEG C of refrigerators；Decapsulated Artemia Cysts are pulverized in mortar spare；Fresh Egg decladding is received
Collection yolk is placed on spare in beaker；According to substance weight each in embodiment, weighs each component in the balance and be uniformly mixed；It will mix
Uniform solution centrifugation is closed, takes supernatant that liquid man-made feeds can be obtained.Man-made feeds loading centrifuge tube is stored in -20 DEG C
Refrigerator is spare.
] embodiment 3
The man-made feeds of the present embodiment, by the material composition of following weight:
It is rice moth egg 12.8g, Decapsulated Artemia Cysts 3.2g, sucrose 2.4g, honey 2.4g, peptone 2.4g, yeast extract 2.4g, new
Fresh yolk 4.8g and water 33.6mL.
Preparation method:
Fresh rice moth egg is chosen into impurity, with mortar grinder at homogenate, beaker is packed into and seals up preservative film to be placed on 4 DEG C of refrigerators spare；
Decapsulated Artemia Cysts are pulverized in mortar spare；Fresh Egg decladding, collection yolk are placed on spare in beaker；According to reality
Each substance weight in example is applied, each component is weighed in the balance and is uniformly mixed；Uniformly mixed solution is centrifuged, supernatant is taken
Obtain liquid man-made feeds.It is spare that man-made feeds loading centrifuge tube is stored in -20 DEG C of refrigerators.
Embodiment 4
The man-made feeds of the present embodiment, by the material composition of following weight ratio:
Fly maggot be homogenized 12.8g, Decapsulated Artemia Cysts 3.2g, sucrose 2.4g, honey 2.4g, peptone 2.4g, yeast extract 2.4g,
Fresh yolk 4.8g and water 33.6mL.
Preparation method:
Fresh fly maggot is taken out from insect box, remove wheat bran after rinsed with clear water, then use 75% alcohol disinfecting, then with clearly
Water repeated flushing, homogenate is fitted into beaker after drying, and seals up preservative film, it is spare to be placed in -4 DEG C of refrigerators.Decapsulated Artemia Cysts are being ground
It pulverizes in alms bowl spare；Fresh Egg decladding, collection yolk are placed on spare in beaker；According to substance weight each in embodiment
Amount, weighs each component in the balance and is uniformly mixed；Uniformly mixed solution is centrifuged, takes supernatant that liquid can be obtained and manually raises
Material.It is spare that man-made feeds loading centrifuge tube is stored in -20 DEG C of refrigerators.
Man-made feeds raise the new small peaceful mite experiment of Pasteur
The new small peaceful mite of Pasteur is raised with the man-made feeds prepared in embodiment 1,2,3,4.Control group 1 uses nature prey two
The raising of variegated leaf mite, the substitution prey ellipse dispersion raising of control group 2.
The new small peaceful mite growth and development of Pasteur, fecundity and survival rate parameter under 1 feeding difference food of table
Rearing conditions: 25 DEG C of temperature, relative humidity 80 ± 5%, illumination 16:8h(L:D)；Comparative example 1,2,3,4 and control 1,2
Initial ovum amount is respectively 114,124,118,120,126,104；The significant difference in development duration and service life uses Tukey's
HSD test；Preoviposition period, the egg-laying season, egg laying amount and immature phase survival rate significant difference bootstrap technology point
Analysis, significance areP < 0.05；Institute's column data is average ± standard error in table, and going together indicates that difference is not shown with letter
It writes.
Conclusion: it can illustrate man-made feeds energy by the new small peaceful mite raising of Pasteur to adult mite with the embodiment of the present invention 1,2,3,4
Enough nutritional needs for effectively meeting the new small peaceful mite of Pasteur.With embodiment 1,2,3 with compare 1,2 feedings, the new small peaceful mite of Pasteur not at
Ripe phase survival rate is without significant difference.In four kinds of man-made feeds, with the new small peaceful mite egg laying amount highest of the Pasteur of the feeding of embodiment 3；
Although comparing with the natural prey of control 1, there are larger differences, compare with 2 substitution prey of control, difference is simultaneously little.
Influence of the man-made feeds that 2 embodiment 3 of table is prepared to the new small peaceful mite development duration of different generations Pasteur
The significant difference of different generations development duration is tested using Tukey's HSD, and significance isP < 0.05；In table
Institute's column data is average ± standard error, and same column indicates that difference is not significant with letter.
Conclusion: carrying out even generation raising to the new small peaceful mite of Pasteur with the man-made feeds prepared in embodiment 3, the results show that bar
Family name's new small peaceful mite 1st generation ovum phase and young mite phase are longer than for the 3rd, 5 generations, and man-made feeds are to the 1st, 3,5 the first deutonymph phases in generation, deutonymph
Phase and immature phase do not make significant difference.Man-made feeds of the present invention at least can satisfy the continuous 5 generation raising of Predatory Mites.
Material used in above-described embodiment, reagent etc. commercially obtain.
Claims (5)
1. a kind of liquid man-made feeds of the new small peaceful mite of Pasteur, which is characterized in that each component including following weight percent:
Basic ingredient accounts for 74-76%, and adding ingredient accounts for 24-26%；, basic ingredient includes the raw material of following mass ratioes: sucrose 5%, honey
5%, peptone 5%, yeast extract 5%, Fresh Egg Huang 10%, water 70%；Adding ingredient includes the raw material of following mass ratioes: 80%
Rice moth egg, 20% Decapsulated Artemia Cysts.
2. the new small peaceful mite liquid man-made feeds of Pasteur according to claim 1, which is characterized in that including following weight percent
Each component of ratio: basic ingredient accounts for 75%, and adding ingredient accounts for 25%.
3. a kind of preparation method of the liquid man-made feeds using the new small peaceful mite of Pasteur such as claims 1 or 2, feature exist
In, comprising the following steps:
1) rice moth egg after impurity will be removed, is ground into homogenate, loading beaker seals up preservative film, and to be placed on refrigerator spare；By decladding
Artemia eggs is pulverized spare；Egg decladding collects yolk and is placed in beaker that seal up preservative film spare；
2) it is matched according to each substance required in man-made feeds, weighs each component in the balance, and be uniformly mixed；
3) uniformly mixed mixed solution is centrifuged, taking supernatant is liquid man-made feeds.
4. the preparation method of the liquid man-made feeds of the new small peaceful mite of Pasteur as described in right 3, which is characterized in that the liquid people
The storage temperature of work feed is -20 DEG C.
5. the preparation method of the liquid man-made feeds of the new small peaceful mite of Pasteur as described in right 3, it is characterised in that: the rice moth egg
Choose impurity, the storage temperature after being ground into homogenate is 4 DEG C.
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| CN201811014967.XA CN109043130B (en) | 2018-08-31 | 2018-08-31 | Liquid artificial feed for neoseiulus pasteurii and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109997791A (en) * | 2019-04-08 | 2019-07-12 | 西南大学 | A kind of new small peaceful mite of Pasteur strain development resistant to high temperature and identification method |
| CN111771820A (en) * | 2020-07-16 | 2020-10-16 | 西南大学 | Method for improving tolerance of neoseiulus barkeri under high-temperature low-humidity co-stress |
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| US20090205057A1 (en) * | 2005-12-29 | 2009-08-13 | Karel Jozef Florent Bolckmans | Mite composition comprising glycyphagidae and phytoseiid mites, use thereof, method for rearing a phytoseiid predatory mite, rearing system for rearing said phytoseiid predatory mite and methods for biological pest control on a crop |
| CN101637149A (en) * | 2009-09-09 | 2010-02-03 | 兰常青 | Production method of predacious mite with characteristic of insect killing and mite killing |
| CN106508812A (en) * | 2016-10-13 | 2017-03-22 | 江西新龙生物科技股份有限公司 | Rapid and efficient propagation method for neoseiulus barkeri |
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2018
- 2018-08-31 CN CN201811014967.XA patent/CN109043130B/en active Active
Patent Citations (3)
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| US20090205057A1 (en) * | 2005-12-29 | 2009-08-13 | Karel Jozef Florent Bolckmans | Mite composition comprising glycyphagidae and phytoseiid mites, use thereof, method for rearing a phytoseiid predatory mite, rearing system for rearing said phytoseiid predatory mite and methods for biological pest control on a crop |
| CN101637149A (en) * | 2009-09-09 | 2010-02-03 | 兰常青 | Production method of predacious mite with characteristic of insect killing and mite killing |
| CN106508812A (en) * | 2016-10-13 | 2017-03-22 | 江西新龙生物科技股份有限公司 | Rapid and efficient propagation method for neoseiulus barkeri |
Non-Patent Citations (2)
| Title |
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| 江高飞等: "巴氏新小绥螨的研究进展", 《中国南方果树》 * |
| 王成斌等: "巴氏新小绥螨在混合猎物系统中的取食选择性研究", 《绿色生态可持续发展与植物保护——中国植物保护学会第十二次全国会员代表大会暨学术年会论文集》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109997791A (en) * | 2019-04-08 | 2019-07-12 | 西南大学 | A kind of new small peaceful mite of Pasteur strain development resistant to high temperature and identification method |
| CN111771820A (en) * | 2020-07-16 | 2020-10-16 | 西南大学 | Method for improving tolerance of neoseiulus barkeri under high-temperature low-humidity co-stress |
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