CN109030451A - Ultrashort pulse time-interleaving degree measuring device and method in CARS micro imaging system - Google Patents
Ultrashort pulse time-interleaving degree measuring device and method in CARS micro imaging system Download PDFInfo
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- 238000003384 imaging method Methods 0.000 title claims abstract description 41
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- 238000003306 harvesting Methods 0.000 claims abstract description 10
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- 239000013307 optical fiber Substances 0.000 claims description 31
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- 239000000126 substance Substances 0.000 claims description 6
- 210000001367 artery Anatomy 0.000 claims description 5
- 239000004038 photonic crystal Substances 0.000 claims description 5
- 210000003462 vein Anatomy 0.000 claims description 5
- 238000004080 punching Methods 0.000 claims description 3
- 238000000691 measurement method Methods 0.000 claims description 2
- 238000005259 measurement Methods 0.000 abstract description 9
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- 238000001069 Raman spectroscopy Methods 0.000 description 5
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- 238000002156 mixing Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/02—Details
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/28—Investigating the spectrum
- G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
- G01J3/4412—Scattering spectrometry
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
- G01N2021/653—Coherent methods [CARS]
Abstract
The invention discloses ultrashort pulse time-interleaving degree measuring device and methods in a kind of CARS micro imaging system, including femto-second laser (1), stokes light pulse optical path, pump light pulse optical path, spectroanalysis instrument (12), focusing objective len (15), three-dimensional automatically controlled objective table (16), light harvesting object lens (17), short pass filter (18) and signal detection and data processing system (19)；Calculate the relative time delay between remnant pump light pulse and soliton as stokes light, that is, time delay of going the same way；The time delay of required adjustable space optical delay line, the road Ji Yi time delay when determining remnant pump light pulse according to the interference spectrum oscillation intensity of remnant pump light pulse and pumping light pulse and pumping optical pulse overlap；When being adjusted according to go the same way time delay and different road delay estimation adjustable space optical delay line in CARS micro imaging system ultrashort pulse time-interleaving degree.The present invention is conducive to the closed loop feedback control of ultrashort pulse time-interleaving in CARS micro imaging system, improves measurement efficiency.
Description
Technical field
The present invention relates to nonlinear fiber optics, impulse time delay control and non-contact spectrum micro-imaging fields, especially
It is related to a kind of device and method of ultrashort pulse time-interleaving degree measurement in CARS micro imaging system.
Background technique
Coherent anti-stokes raman scattering (CoherentAnti-Stokes Raman Scattering, CARS) is micro-
Imaging technique is a kind of non-contact, unmarked microtechnic that the resonance level information using molecule in substance is imaged.
The essence of CARS micro-imaging technique is pump light, the four-wave mixing effect for detecting light, sample to be tested resonance level, wherein pumping
The frequency difference of light and stokes light is equal to the target chemical bond resonance energy grade of sample to be tested, when pump light and stokes light simultaneously
Sample to be tested is focused to, and when three meets phase-matching condition, the anti-Stokes Raman scattering of sample to be tested will be inspired
Signal, i.e. CARS signal.Since CARS signal is coherent signal and relative to pump light and stokes light blue shift, have
Conducive to the interference for filtering out sample to be tested fluorescence background, the contrast of micro imaging system is improved.CARS signal is non-based on three ranks
The four-wave mixing effect of linear process and generate, so only focal spot very a small range generate, have essence three
Tie up chromatography ability.Therefore CARS micro-imaging technique is non-in active somatic cell vital movement, protein formation, the transmission of cell adipose membrane etc.
Contact area field has a wide range of applications.
Currently, in order to improve the performance of CARS micro imaging system, such as time resolution, partially is had been proposed in researcher
The technologies such as vibration control, relevant control, Fourier transformation, frequency modulation(PFM), double light combs.In CARS micro-imaging technique, pump light
The time-interleaving of pulse and Stokes light pulse is vital, usually used optical delay line or stepper motor displacement platform reality
Now time-interleaving between the two, but since the time width of pumping light pulse and Stokes light pulse is very narrow, it is only several
Hundred femtoseconds directly pass through photodetector and oscillograph detection pumping light pulse and Stokes light pulse to several picoseconds
Time-interleaving is very difficult.Simultaneously as propagation delay time in the CARS micro imaging system based on soliton self-frequency sh effect
Wavelength dependence, when the complete Raman resonance line to sample to be tested measures, need according to pumping light pulse and this
The relative time delay that the frequency difference of lentor light pulse both constantly regulate pumps light pulse and Stokes to be overlapped between guaranteeing
Time-interleaving between light pulse haves the shortcomings that tuning is difficult, regulating time is long.
However in few documents CARS micro imaging systems ultrashort pulse time-interleaving problem, at present it is existing
Optical delay line tuning is still large area scanning and according to whether CARS signal occurs as pumping light pulse and stokes light
The foundation of burst length overlapping.Therefore existing delay adjusting mode is unfavorable for pulse time-interleaving in CARS micro imaging system
Closed loop feedback control and system automatic measurement, reduce its use value in practical applications.The present invention relates to
The device and method of ultrashort pulse time-interleaving degree measurement, are based on general non-linear Schrodinger in a kind of CARS micro imaging system
The theoretical calculation of equation and the interference spectrum of ultrashort pulse are estimated and pump light pulse and Stokes in CARS micro imaging system
Required time delay when optical pulse time is overlapped, proposition of the invention are conducive to the efficient of delay adjusting in CARS micro imaging system
Automation carries out, and improves systematic survey efficiency, reduces system preliminary preparation, promotes CARS micro imaging system in practice
Application.
Summary of the invention
Ultrashort pulse time-interleaving degree measuring device and method in a kind of CARS micro imaging system proposed by the present invention, fill
Divide using the remnant pump light in high non-linear photon crystal optical fiber output light, be used as intermediary, realizes time-interleaving
Degree measurement.
The present invention proposes ultrashort pulse time-interleaving degree measuring device in a kind of CARS micro imaging system, which includes
Femto-second laser 1, electrically-controlled liquid crystal wave plate 2, polarization beam splitter prism 3, stokes light pulse optical path, pump light pulse optical path, light
Spectrum analysis instrument 12, third reflecting mirror 13, long pass filter 14, focusing objective len 15, three-dimensional automatically controlled objective table 16, light harvesting object lens 17,
Short pass filter 18 and signal detection and data processing system 19；Wherein:
The femto-second laser 1 is connect with the input terminal of electrically-controlled liquid crystal wave plate 2, the output end of the electrically-controlled liquid crystal wave plate 2
Connect with the polarization beam splitter prism 3,3 output end of polarization beam splitter prism be respectively the stokes light pulse optical path and
The pump light pulse optical path, wherein the stokes light pulse optical routing fibre-coupled mirrors 4, high non-linear photon crystal light
Fibre 5, fiber optic collimator beam expander 6 and bundling device 11 sequentially connect and compose；The first reflecting mirror of the pump light pulse optical routing 7,
One, the adjustable space optical delay line 8, second that the second right-angle reflecting prism 81,82 and high precision electric control displacement platform 83 form reflects
Mirror 9, half wave plate 10 and bundling device 11 sequentially connect and compose；The output of the bundling device 11 divides two-way, all the way with spectrum
Analyzer 12 connects, and another way is connect with third reflecting mirror 13, the third reflecting mirror 13 and long pass filter 14, focusing objective len
15, three-dimensional automatically controlled objective table 16, light harvesting object lens 17, short pass filter 18 and signal detection and data processing system 19 sequentially connect
It connects；
1 output pulse width of femto-second laser is the femtosecond pulse of hundred femtosecond magnitudes, horizontal linear polarization, through electrically-controlled liquid crystal wave
Polarization beam splitter prism 3 is incident to after piece 2, incident light pulse is divided into horizontal polarization light all the way and erected all the way by polarization beam splitter prism 3
Straight polarised light；
In the stokes light pulse optical path, horizontal polarization light is coupled into high non-linearity photon through fibre-coupled mirrors 4
Crystal optical fibre 5, high non-linear photon crystal optical fiber 5 are used as CARS micro-imaging system since soliton self-frequency sh effect generates soliton
The output light of the Stokes light pulse of system, high non-linear photon crystal optical fiber 5 is incident to bundling device through fiber optic collimator beam expander 6
The 11 vertical polarised lights described in the pump light pulse optical routing are incident to adjustable space optical delay line 8 through the first reflecting mirror 7, the
One right-angle reflecting prism 81 reflects incident pump light pulse-echo to the second right angle for being fixed on high precision electric control displacement platform 83
Mirror 82, while the reflected light pulse of the second right-angle reflecting prism 82 is passed through into its another right-angle side reflection output, high-precision
Automatically controlled displacement platform 83 is for changing the time delay for pumping optical path, it is ensured that the time of pumping light pulse and Stokes light pulse weight
It is folded；The pumping light pulse that adjustable space optical delay line 8 exports successively is incident to conjunction through the second reflecting mirror 9, half wave plate 10
Beam device 11；
The spectroanalysis instrument 12 is used to receive the output light of the high non-linear photon crystal optical fiber 5 reflected by bundling device 11
With the pumping light pulse of transmission, the remnant pump light pulse in 5 output light of high non-linear photon crystal optical fiber and pump light are detected
The interference spectrum of pulse；
Transmitted light of the Stokes light pulse through the bundling device 11 and pumping reflection of the light pulse through the bundling device 11
After combiner, focusing objective len 15, the automatically controlled load of three-dimensional are incident to through the third reflecting mirror 13 and the long pass filter 14
After the CARS signal of sample to be tested transmission on object platform 16 is collected by the light harvesting object lens 17, filtered out through the short pass filter 18
Stray light is incident to the signal detection and data processing system 19.
Ultrashort pulse time-interleaving degree measurement method, this method are at least wrapped in a kind of CARS micro imaging system of the invention
Include following steps:
Step 1, the time delays for calculating soliton and remnant pump light pulse caused by soliton self-frequency sh effect, i.e., together
Road delay, τ1:
τ1=τsolution-τpump
Wherein, τsolutionIndicate the time delay of orphan, τpumpIndicate the time delay of incident pump pulse；
Step 2, horizontal polarization femtosecond pulse through electrically-controlled liquid crystal wave plate realize polarization direction rotation, polarization direction rotation
Femtosecond pulse afterwards is divided into the continuously adjustable horizontal polarization light pulse of power and vertical polarized optical pulse through polarization beam splitter prism；
Step 3, horizontal polarization light pulse enter high non-linear photon crystal optical fiber through fibre-coupled mirrors, will be by high non-linearity
The soliton for the tunable wave length that the soliton self-frequency sh effect of photonic crystal fiber generates is as Stokes light pulse；It is vertical inclined
Light shake successively through the first reflecting mirror, adjustable space optical delay line, the second reflecting mirror and half wave plate, realizes pumping light pulse
Time delays adjust and polarize by vertical direction rotate to be horizontal direction adjust；
The reflected light for transmiting light pulse and Stokes light pulse economic cooperation beam device of step 4, pumping light pulse economic cooperation beam device
Pulse is incident to spectroanalysis instrument, the interference light of remnant pump light and pump light that detection high non-linear photon crystal optical fiber generates
Spectrum, the amount of delay of adjustable space optical delay line when determining remnant pump light pulse and pumping optical pulse time overlapping, when the road Ji Yi
Prolong τ2:
τ2=τ (max [∫ | I (λ, τ)-Iref(λ,τ)|dλ])
Wherein, I (λ, τ) indicates variation of the interference spectrum amplitude with wavelength X and delay, τ, Iref(λ, τ) indicates remnant pump
The reference spectra information that spectroanalysis instrument receives when light pulse and pump light pulse are located remotely from each other；
Step 5), according to going the same way delay, τ1With different road delay, τ2, estimate and pump light pulse and this in CARS micro imaging system
Amount of delay τ needed for the overlapping of lentor optical pulse time:
τ=τ1+τ2
And adjustable space optical delay line is adjusted, make to pump light pulse and time-interleaving is realized in Stokes light pulse；
Step 6, by the pumping light pulse of time-interleaving and Stokes light pulse through third reflecting mirror, long pass filter and
It is incident to sample to be tested after focusing objective len, excites the CARS signal at sample to be tested different spatial；Sample to be tested is collected to produce
Raw CARS signal and after short pass filter filters out stray light analyzes the power and spatial distribution of CARS signal, reconstructs to be measured
The spatial distribution of performance chemical key in sample.
Compared with prior art, ultrashort pulse time-interleaving degree measurement dress in a kind of CARS micro imaging system of the invention
The closed loop feedback control for being conducive to ultrashort pulse time-interleaving in CARS micro imaging system with method is set, system automation is improved
Degree and measurement efficiency reduce system preliminary preparation, promote the application of CARS micro imaging system in practice.
Appended drawing reference
Fig. 1 is that light pulse and Stokes optical pulse time overlapping estimation schematic diagram are pumped in CARS micro imaging system；
Fig. 2 is go the same way time delay and its linear fit result calculated based on general non-linear Schrodinger equation；
Fig. 3 is the structure drawing of device of ultrashort pulse time-interleaving degree measurement in CARS micro imaging system of the invention.
Appended drawing reference:
1, femto-second laser, 2, electrically-controlled liquid crystal wave plate, 3, polarization beam splitter prism, 4, fibre-coupled mirrors, 5, high non-linearity light
Photonic crystal fiber, 6, fiber optic collimator beam expanding lens, the 7, first reflecting mirror, 8, adjustable space optical delay line, the 9, second reflecting mirror, 10,
Half wave plate, 11, bundling device, 12, spectroanalysis instrument, 13, third reflecting mirror, 14, long pass filter, 15, focusing objective len,
16, three-dimensional automatically controlled objective table, 17, light harvesting object lens, 18, short pass filter, 19, signal detection and data processing system；
81, the first right-angle reflecting prism, the 82, second right-angle reflecting prism, 83, high precision electric control displacement platform.
Specific embodiment
Embodiments of the present invention are described in further detail below in conjunction with attached drawing.
As shown in Figure 1, to pump light pulse and the overlapping estimation of Stokes optical pulse time in CARS micro imaging system
Schematic diagram.Femtosecond pulse output is divided into two bundles, a branch of for pumping high non-linear photon crystal optical fiber, by soliton self-frequency sh effect
Stokes light of the soliton of generation as CARS micro imaging system, and the remnants of high non-linear photon crystal optical fiber output
The intermediary that light pulse is used as time-interleaving degree measurement in system is pumped, another beam femtosecond pulse is used as CARS micro-imaging system
The pumping light pulse of system.First by remaining in general non-linear Schrodinger equation theoretical calculation high non-linear photon crystal optical fiber
Pump the relative time delay amount of light pulse and soliton, that is, delay, τ of going the same way1；Then according to remnant pump light pulse and pump light arteries and veins
It is punched in the interference spectrum when scanning of adjustable space optical delay line, remnant pump light pulse is determined according to the oscillation intensity of interference spectrum
With delay volume when pumping optical pulse time overlapping, the road Ji Yi delay, τ2；According to delay, τ of going the same way1With different road delay, τ2, can be true
The time weight of light pulse and Stokes light pulse is pumped when determining the scanning of adjustable space optical delay line in CARS micro imaging system
Folded degree.
Wherein, it goes the same way delay, τ1Following (the τ of calculation formula1=τsoliton-τpump, τsolitonIndicate the time delay of orphan, τpumpTable
It is shown into the time delay for penetrating pumping pulse):
Soliton and remnant pump light in high non-linear photon crystal optical fiber are calculated according to general non-linear Schrodinger equation
The relative time delay amount of pulse, general non-linear Schrodinger equation are as follows:
Wherein, A indicates that light wave amplitude, z indicate transmission range, β2Indicate the second-order dispersion parameter of optical fiber, β3Indicate optical fiber
Third-order dispersion parameter, α indicate the decaying of optical fiber, and γ indicates the nonlinear factor of optical fiber, TRIndicate Raman response, ω0Indicate incident
The central wavelength of light wave, T indicate the time variable in the coordinate system that optical fiber moves radially, vgIndicate group velocity.
Different road delay, τ2Calculation formula:
τ2=τ (max [∫ | I (λ, τ)-Iref(λ,τ)|dλ])
Remnant pump light pulse and pumping are calculated according to the interference spectrum of remnant pump light pulse and Stokes light pulse
Optical pulse time overlapping, theoretical foundation are as follows:
Wherein, I (λ, τ) indicates variation of the interference spectrum amplitude with wavelength X and delay, τ, I0(λ) indicates remnant pump light arteries and veins
The original spectrum of punching and pumping light pulse, to simplify the analysis, it is assumed that remnant pump light pulse and Stokes light pulse have phase
The spectrum of similar shape and amplitude.Iref(λ, τ) indicates spectroanalysis instrument when remnant pump light pulse and pump light pulse are located remotely from each other
The reference spectra information received.
As shown in figure 3, for ultrashort pulse time-interleaving degree measuring device structure in CARS micro imaging system of the invention
Schematic diagram.The device includes femto-second laser 1, electrically-controlled liquid crystal wave plate 2, polarization beam splitter prism 3, fibre-coupled mirrors 4, high non-linearity
Photonic crystal fiber 5, fiber optic collimator beam expander 6, the first reflecting mirror 7, adjustable space optical delay line 8 (reflect rib by the first right angle
Mirror 81, the second right-angle reflecting prism 82 and high precision electric control displacement platform 83 form), the second reflecting mirror 9, half wave plate 10,
Bundling device 11, spectroanalysis instrument 12, third reflecting mirror 13, long pass filter 14, focusing objective len 15, three-dimensional automatically controlled objective table 16,
Light harvesting object lens 17, short pass filter 18 and signal detection and data processing system 19.
1 output pulse width of femto-second laser is the femtosecond pulse of hundred femtosecond magnitudes, horizontal linear polarization, through electrically-controlled liquid crystal wave plate 2
After be incident to polarization beam splitter prism 3, polarization beam splitter prism 3 by incident light pulse be divided into horizontal polarization light all the way and all the way it is vertical partially
Shake light:
Horizontal polarization light is coupled into high non-linear photon crystal optical fiber 5, high non-linear photon crystal light through fibre-coupled mirrors 4
The output light of fibre 5 is incident to bundling device 11 through fiber optic collimator beam expander 6.
Vertical polarised light is incident to adjustable space optical delay line 8 through the first reflecting mirror 7, and adjustable space optical delay line 8 is by two
A right-angle reflecting prism 81,82 and high precision electric control displacement platform 83 form, and the first right-angle reflecting prism 81 is by incident pump
Light pulse reflexes to the second corner cube mirror 82 for being fixed on high precision electric control displacement platform 83, while by the second right-angle reflecting prism
82 reflected light pulses are by its another right-angle side reflection output, and high precision electric control displacement platform 83 is for changing pumping optical path
Time delay.The pumping light pulse that adjustable space optical delay line 8 exports is successively through the second reflecting mirror 9, half wave plate 10
It is incident to bundling device 11.
Spectroanalysis instrument 12 is used to receive the output light of the high non-linear photon crystal optical fiber 5 reflected by bundling device 11 and saturating
The pumping light pulse penetrated.
Transmitted light and pump light reflection through bundling device 11 of 5 output light of high non-linear photon crystal optical fiber through bundling device 11
After combiner, it is incident to focusing objective len 15 through third reflecting mirror 13 and long pass filter 14, sample to be tested is placed on conglomeration
On the automatically controlled objective table 16 of three-dimensional at mirror foci, after being collected by the CARS signal of sample to be tested transmission by light harvesting object lens 17, through short
Pass filter 18 is incident to the signal detection and data processing system 19 being made of photomultiplier tube and data collecting card.
The method that ultrashort pulse time-interleaving degree measures in a kind of CARS micro imaging system of the invention, this method are main
The following steps are included:
Step 1, according to the parameter of general non-linear Schrodinger equation and high non-linear photon crystal optical fiber, calculate orphan from
The time delays of soliton caused by frequency shift effect and remnant pump light pulse, that is, delay, τ of going the same way1, calculated result such as Fig. 2
It is shown.As can be seen that when soliton wavelength is gradually increased to 1300nm from 800nm, delay, τ of going the same way1It is gradually increased to from 1ps
45ps；
The femtosecond pulse for the horizontal linear polarization that step 2, femtosecond pulse laser export is realized through electrically-controlled liquid crystal wave plate to be polarized
The rotation in direction, and its rotation angle is related with the modulation voltage that electrically-controlled liquid crystal wave plate is applied.Polarization direction is postrotational to fly
Pulse per second (PPS) is divided into the continuously adjustable horizontal polarization light pulse of power and vertical polarized optical pulse through polarization beam splitter prism；
Step 3, wherein a branch of horizontal polarization light pulse enter high non-linear photon crystal optical fiber through fibre-coupled mirrors, will be by
The soliton for the tunable wave length that the soliton self-frequency sh effect of high non-linear photon crystal optical fiber generates is as stokes light arteries and veins
Punching；Another Shu Shuzhi polarised light is successively through the first reflecting mirror, adjustable space optical delay line, the second reflecting mirror and half wave
Piece realizes that the time delays of pumping light pulse are adjusted and polarized and rotates to be horizontal direction adjusting by vertical direction.
The reflection light pulse of step 4, pump beam transmission light pulse and Stokes light beam economic cooperation beam device through bundling device
It is incident to spectroanalysis instrument, the remnant pump light of detection high non-linear photon crystal optical fiber generation and the interference spectrum of pump light,
Adjustable space optical delay line when determining remnant pump light pulse according to the intensity of interference spectrum and pumping optical pulse time overlapping
Amount of delay, the road Ji Yi delay, τ2；
Step 5, according to delay, τ calculated of going the same way1With measured different road delay, τ2It estimates in CARS micro imaging system
Amount of delay needed for pumping light pulse and the overlapping of Stokes optical pulse time, and adjustable space optical delay line is adjusted accordingly,
Make to pump light pulse and time-interleaving is realized in Stokes light pulse；
Step 6, by the pumping light pulse of time-interleaving and Stokes light pulse through third reflecting mirror, long pass filter and
Sample to be tested is incident to after focusing objective len.Excite the CARS signal at sample to be tested different spatial.Sample to be tested is collected to produce
Raw CARS signal and after short pass filter filters out stray light analyzes the power and spatial distribution of CARS signal, reconstructs to be measured
The spatial distribution of performance chemical key in sample.
Claims (2)
1. ultrashort pulse time-interleaving degree measuring device in a kind of CARS micro imaging system, which is characterized in that the device includes
Femto-second laser (1), electrically-controlled liquid crystal wave plate (2), polarization beam splitter prism 3, stokes light pulse optical path, pump light pulsed light
Road, spectroanalysis instrument (12), third reflecting mirror (13), long pass filter (14), focusing objective len (15), three-dimensional automatically controlled objective table
(16), light harvesting object lens (17), short pass filter (18) and signal detection and data processing system (19)；Wherein:
The femto-second laser (1) connect with the input terminal of electrically-controlled liquid crystal wave plate (2), the output of the electrically-controlled liquid crystal wave plate (2)
End is connect with the polarization beam splitter prism (3), and polarization beam splitter prism (3) output end is the Stokes light pulse respectively
Optical path and the pump light pulse optical path, wherein the stokes light pulse optical routing fibre-coupled mirrors (4), high non-linearity light
Photonic crystal fiber (5), fiber optic collimator beam expander (6) and bundling device (11) sequentially connect and compose；The pump light pulse optical routing
First reflecting mirror (7), the first, second right-angle reflecting prism (81), (82) and high precision electric control displacement platform (83) form adjustable
Space optical delay line (8), the second reflecting mirror (9), half wave plate (10) and bundling device (11) sequentially connect and compose；The conjunction
The output of beam device (11) divides two-way, connect all the way with spectroanalysis instrument (12), another way is connect with third reflecting mirror (13), described
Third reflecting mirror (13) and long pass filter (14), focusing objective len (15), three-dimensional automatically controlled objective table (16), light harvesting object lens (17),
Short pass filter (18) and signal detection and data processing system (19) sequentially connect；
Femto-second laser (1) output pulse width is the femtosecond pulse of hundred femtosecond magnitudes, horizontal linear polarization, through electrically-controlled liquid crystal wave plate
(2) it is incident to after polarization beam splitter prism (3), incident light pulse is divided into horizontal polarization light and one all the way by polarization beam splitter prism (3)
The vertical polarised light in road；
In the stokes light pulse optical path, horizontal polarization light is coupled into high non-linearity photon crystalline substance through fibre-coupled mirrors (4)
Body optical fiber (5), high non-linear photon crystal optical fiber (5) are used as CARS micro-imaging since soliton self-frequency sh effect generates soliton
The output light of the Stokes light pulse of system, high non-linear photon crystal optical fiber (5) is incident to through fiber optic collimator beam expander (6)
Bundling device (11)；
Through the first reflecting mirror, (7 are incident to adjustable space optical delay line to the vertical polarised light described in the pump light pulse optical routing
(8), the first right-angle reflecting prism (81) is by incident pump light pulse-echo to being fixed on the of high precision electric control displacement platform (83)
Two corner cube mirrors (82), at the same the second right-angle reflecting prism (82) reflected light pulse is anti-by its another right-angle side
Output is penetrated, high precision electric control displacement platform 83 is for changing the time delay for pumping optical path, it is ensured that pumping light pulse and Stokes
The time-interleaving of light pulse；The pumping light pulse of adjustable space optical delay line (8) output is successively through the second reflecting mirror (9), two points
One of wave plate (10) be incident to bundling device (11)；
The spectroanalysis instrument (12) is used to receive the output of the high non-linear photon crystal optical fiber (5) by bundling device (11) reflection
The pumping light pulse of light and transmission detects high non-linear photon crystal optical fiber (remnant pump light pulse and pumping in 5 output lights
The interference spectrum of light pulse；
Transmitted light of the Stokes light pulse through the bundling device (11) and pumping light pulse are through the bundling device (11 reflected light
Close beam after, through the third reflecting mirror (13 and the long pass filter (14) be incident to focusing objective len (15), the three-dimensional is automatically controlled
After the CARS signal of sample to be tested transmission on objective table (16) is collected by the light harvesting object lens (17), through the short pass filter
(18) stray light is filtered out, the signal detection and data processing system (19) are incident to.
2. ultrashort pulse time-interleaving degree measurement method in a kind of CARS micro imaging system, which is characterized in that this method is at least
The following steps are included:
Step (1), the time delays for calculating soliton and remnant pump light pulse caused by soliton self-frequency sh effect, that is, go the same way
Delay, τ1,
τ1=τsolution-τpump
Wherein, τsolutionIndicate the time delay of orphan, τpumpIndicate the time delay of incident pump pulse；
Step (2), horizontal polarization femtosecond pulse through electrically-controlled liquid crystal wave plate realize polarization direction rotation, polarization direction rotation after
Femtosecond pulse be divided into the continuously adjustable horizontal polarization light pulse of power and vertical polarized optical pulse through polarization beam splitter prism；
Step (3), horizontal polarization light pulse enter high non-linear photon crystal optical fiber through fibre-coupled mirrors, will be by high non-linearity light
The soliton for the tunable wave length that the soliton self-frequency sh effect of photonic crystal fiber generates is as Stokes light pulse；Vertical polarization
Light successively through the first reflecting mirror, adjustable space optical delay line, the second reflecting mirror and half wave plate, realizes pumping light pulse
Time delays, which are adjusted and polarized, rotates to be horizontal direction adjusting by vertical direction；
The reflected light arteries and veins for transmiting light pulse and Stokes light pulse economic cooperation beam device of step (4), pumping light pulse economic cooperation beam device
It pours and is incident upon spectroanalysis instrument, the interference light of remnant pump light and pump light that detection high non-linear photon crystal optical fiber generates
Spectrum, the amount of delay of adjustable space optical delay line when determining remnant pump light pulse and pumping optical pulse time overlapping, when the road Ji Yi
Prolong τ2,
τ2=τ (max [∫ | I (λ, τ)-Iref(λ,τ)|dλ])
Wherein, I (λ, τ) indicates variation of the interference spectrum amplitude with wavelength X and delay, τ, Iref(λ, τ) indicates remnant pump light arteries and veins
The reference spectra information that spectroanalysis instrument receives when punching and pump light pulse are located remotely from each other；
Step (5), according to going the same way delay, τ1With different road delay, τ2, estimate and pump light pulse and Si Tuo in CARS micro imaging system
Amount of delay τ needed for the overlapping of gram this optical pulse time:
τ=τ1+τ2
And adjustable space optical delay line is adjusted, make to pump light pulse and time-interleaving is realized in Stokes light pulse；
Step (6) through third reflecting mirror, long pass filter and gathers the pumping light pulse of time-interleaving and Stokes light pulse
It is incident to sample to be tested after focus objective lens, excites the CARS signal at sample to be tested different spatial；Sample to be tested is collected to generate
CARS signal and after short pass filter filters out stray light, analyze CARS signal power and spatial distribution, reconstruct to test sample
The spatial distribution of performance chemical key in product.
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