CN109030165A - A kind of vortex evenly mixing device for microbial endotoxins detection - Google Patents
A kind of vortex evenly mixing device for microbial endotoxins detection Download PDFInfo
- Publication number
- CN109030165A CN109030165A CN201810708548.XA CN201810708548A CN109030165A CN 109030165 A CN109030165 A CN 109030165A CN 201810708548 A CN201810708548 A CN 201810708548A CN 109030165 A CN109030165 A CN 109030165A
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- fixed
- disk
- test tube
- motor
- cabinet
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
- G01N2001/386—Other diluting or mixing processes
Abstract
The invention discloses a kind of vortex evenly mixing device for microbial endotoxins detection, including cabinet, box house is fixed with motor, and motor clamps mixing disk by transmission mechanism with test tube respectively and the fixed disk that mixes of beaker is connect；The bottom insertion of cabinet is fixed with step-like cushion blocking, fixed backing plate is welded on box house bottom plate, motor is fixed on fixed backing plate by foundation bolt, vibration damping noise control interlayer is equipped between motor and fixed backing plate, driving gear is fixed on main shaft, the side of main shaft is equipped with transmission gear, driving gear and transmission gear are intermeshed, the fixed mixing disk of beaker and test tube clamping mix disk and are individually fixed in above the limit stop ring of main shaft and drive shaft, and test tube clamping mixing disk is equipped with two layers.The device every batch of can handle more measurement samples, reduce operating quantity, reduce the detection time interval between the sample of front and back, further reduced the error of testing result, while can reduce injury of the noise to testing staff.
Description
Technical field
The present invention relates to medical detection technologies, and in particular to a kind of vortex mixing for microbial endotoxins detection
Device.
Background technique
Detection of bacterial endotoxin is to judge whether bacterial endotoxin meets regulation, endotoxin, that is, Gram-negative in test sample
The lipopolysaccharides of bacterium cell wall, toxic component are lipoid A, are released after thallus death disintegration.Detection of bacterial endotoxin includes
Two methods, i.e. gel method and photometry, the latter include nephelometry and chromogenic substrate method.
The gel genealogy of law is detected by reagents and the principle of endotoxin generation agglutinating reaction or the endotoxic side of sxemiquantitative
Method.According to the sign value (λ) of sensitivity of the limulus reagent, bacterial endotoxin national standard or bacterial endotoxin working standard are used
Baterial endotoxin test is dissolved with water, is mixed 15 minutes on vortex mixer, and 2 λ, λ, 0.5 λ and 0.25 λ tetra- is then made
The endotoxin standard solution of concentration, one step of every dilution should all mix 30 seconds on vortex mixer.
However since the ability restriction of existing blending instrument instrument sample per treatment and manual operations are cumbersome, operating
The time interval that sample successively detects in the process is excessive, greatly affected the accurate rate of testing result, existing blending instrument is only
There is a rotatable platform seriously to constrain the ability of sample per treatment, and works as and need replacing different mixing disks to be respectively completed
The mixing operation of sample in test tube and beaker, and detect the sample examination of the sample for needing to handle four kinds of various concentrations and every kind of concentration
Pipe just has as many as 18, and testing staff's a large amount of time, which is all spent, mixes the mistake of the repetitive operation of disk in upper and lower test tube and replacement
Cheng Zhong seriously reduces detection efficiency；When existing blending instrument is switched from low speed to high-speed rotation and is mixed with high-speed rotation,
Noise is very big, and existing blending instrument sound insulation and noise reduction ability is very poor, and in high-speed rotation, and the pedestal of blending instrument easily vibrates,
The noise in detection process is further increased, and noise is in the injury pole in the environment of noise to testing staff for a long time
Greatly.
Summary of the invention
The present invention to solve the above-mentioned problems, devises a kind of vortex evenly mixing device for microbial endotoxins detection,
The device every batch of can handle more measurement samples, while every batch of can handle the different containers to contain sample simultaneously
Mixing operation, while reducing operating quantity, reduce the detection time interval between the sample of front and back, further reduced inspection
The error of result is surveyed, the present apparatus can also carry out vibration damping, vibration isolation to the noise generated in blending process from inside configuration and disappear to reach
Except the purpose of noise, injury of the noise to testing staff is reduced.
In order to achieve the above technical purposes, reach above-mentioned technical effect, the present invention is achieved by the following technical solutions:
A kind of vortex evenly mixing device for microbial endotoxins detection, which is characterized in that including cabinet, box house is solid
Surely there is motor, motor clamps mixing disk by transmission mechanism with test tube respectively and the fixed disk that mixes of beaker is connect；
The shell of the cabinet is equipped with motor switch button and motor fm dial, and the side of cabinet is equipped with electricity
The bottom insertion of source plug, cabinet is fixed with step-like cushion blocking, and fixed backing plate is welded on box house bottom plate, and motor is logical
It crosses foundation bolt to be fixed on fixed backing plate, is equipped with vibration damping noise control interlayer, the top of motor between motor and fixed backing plate
It is equipped with partition between the top plate of cabinet, is equipped with interlayer cavity between partition and top, the main shaft of motor is from partition and top
It is stretched out at the center of plate, driving gear is fixed on the main shaft in interlayer cavity, the side of main shaft is equipped with transmission gear, passes
The radius of moving gear and the radius of driving gear are equal, and driving gear and transmission gear are intermeshed, and transmission gear passes through transmission
Shaft is fixed in interlayer cavity, and one end of drive shaft is fixed on the thrust ball bearing on partition and the other end is from cabinet
It is stretched out on top plate；
The extension of the main shaft and drive shaft is equipped with limit stop ring, and the fixed mixing disk of beaker and test tube clamping are mixed
Even disk is individually fixed in above the limit stop ring of main shaft and drive shaft, and test tube clamping mixes disk and is equipped with two layers, the examination of lower layer
Tube grip mixes and is fixed with height adjustment column on disk, and the test tube clamping on upper layer mixes disk and is fixed on height adjustment column.
Preferably, the drive shaft is equipped with 4 and is uniformly distributed within one week around main shaft, beaker is fixed mix disk and
The test tube clamping of lower layer mixes disk and is threadedly coupled respectively with main shaft and drive shaft, and the test tube clamping of lower layer mixes at disk center
It is provided with thread groove, height adjustment column mixes disk with the clamping of the test tube of lower layer and is threadedly coupled, and the test tube clamping on upper layer mixes in disk
Tapped through hole is provided at the heart, the test tube clamping on upper layer mixes disk and connect with height adjustment male screw, and test tube clamping mixes the disk of disk
It is provided with test tube clamp groove on face, is provided with beaker fixed groove in the fixed disk for mixing disk of beaker.
Preferably, the cabinet and partition are an integral structure, and cabinet and partition are the double-deck plate body structure, cabinet and every
Mandruka is filled in the interlayer of plate.
Preferably, the cushion blocking and vibration damping noise control interlayer are made of rubber material, and cabinet uses stainless steel metal
Material is made.
The beneficial effects of the present invention are: the device every batch of can handle more measurement samples, operating quantity is reduced, is reduced
Detection time interval between the sample of front and back further reduced the error of testing result, while same using multiple rotatable platforms
When handle the different mixings to contain the container of sample and operate, and the rotational angular velocity of each rotatable platform is identical, guarantees
The sample that each test tube is mixed with identical treatment conditions, the present apparatus can also be from inside configurations to generating in blending process
Noise carry out vibration damping, vibration isolation to achieve the purpose that eliminate noise, reduce injury of the noise to testing staff.
Detailed description of the invention
In order to illustrate the technical solution of the embodiments of the present invention more clearly, will be described below to embodiment required
Attached drawing is briefly described, it should be apparent that, drawings in the following description are only some embodiments of the invention, for ability
For the those of ordinary skill of domain, without creative efforts, it can also be obtained according to these attached drawings other attached
Figure.
Fig. 1 is a kind of surface structure schematic diagram of vortex evenly mixing device for microbial endotoxins detection；
Fig. 2 is a kind of schematic diagram of internal structure of vortex evenly mixing device for microbial endotoxins detection；
Fig. 3 is a kind of overlooking structure diagram of vortex evenly mixing device for microbial endotoxins detection.
In attached drawing, parts list represented by the reference numerals are as follows:
1- cabinet, 2- motor, 3- transmit shaft, and 4- height adjustment column, 5- beaker is fixed to mix disk, and the clamping of 6- test tube is mixed
Even disk, 11- cushion blocking, 12- attaching plug, 13- motor switch button, 14- motor fm dial, 21- main shaft, 22-
Driving gear, 31- limit stop ring, 32- transmission gear, 33- thrust ball bearing, 51- beaker fixed groove, the clamping of 61- test tube are recessed
Slot, the fixed backing plate of 101-, 102- vibration damping noise control interlayer, 103- foundation bolt.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts all other
Embodiment shall fall within the protection scope of the present invention.
Refering to fig. 1 shown in -3, a kind of vortex evenly mixing device for microbial endotoxins detection, including cabinet 1, cabinet 1
Inside is fixed with motor 2, and motor 2 clamps mixing disk 6 by transmission mechanism with test tube respectively and the fixed disk 5 that mixes of beaker connects
It connects；
The shell of the cabinet 1 is equipped with motor switch button 13 and motor fm dial 14, the side of cabinet 1
Equipped with attaching plug 12, the bottom insertion of cabinet 1 is fixed with step-like cushion blocking 11, is welded with fixed pad on box house bottom plate
Plate 101, motor 2 are fixed on fixed backing plate 101 by foundation bolt 103, are equipped between motor 2 and fixed backing plate 101
Vibration damping noise control interlayer 102 is equipped with partition between the top of motor 2 and the top plate of cabinet, interlayer is equipped between partition and top
Cavity, the main shaft 21 of motor stretch out from the center of partition and top plate, are fixed with master on the main shaft 21 in interlayer cavity
The side of moving gear 22, main shaft 21 is equipped with transmission gear 32, and the radius of transmission gear 32 is equal with the radius of driving gear 22,
Driving gear 22 and transmission gear 32 are intermeshed, and transmission gear 32 is fixed in interlayer cavity by drive shaft 3, and transmission turns
On the thrust ball bearing 33 that one end of axis 3 is fixed on partition and the other end is stretched out from the top plate of cabinet 1；
The extension of the main shaft 21 and drive shaft 3 is equipped with limit stop ring 31, and beaker is fixed to mix disk 5 and test tube
Clamping mixes 31 top of limit stop ring that disk 6 is individually fixed in main shaft 21 and drive shaft 3, and test tube clamping mixes disk 6 and is equipped with
Two layers, the test tube clamping of lower layer, which mixes, is fixed with height adjustment column 4 on disk 6, the test tube clamping on upper layer mixes disk 6 and is fixed on height
On adjustable column 4.
Therein, the drive shaft 3 is equipped with 4 and is uniformly distributed within 21 1 weeks around main shaft, and beaker is fixed to be mixed
Disk 5 and the clamping of the test tube of lower layer mix disk 6 and are threadedly coupled respectively with main shaft 21 and drive shaft 3, and the test tube clamping of lower layer is mixed
Thread groove is provided at even 6 center of disk, height adjustment column 4 mixes disk 6 with the clamping of the test tube of lower layer and is threadedly coupled, the test tube on upper layer
Clamping mixes and is provided with tapped through hole at 6 center of disk, and the test tube clamping on upper layer mixes disk 6 and is threadedly coupled with height adjustment column 4, test tube
It is provided with test tube clamp groove 61 in the disk of clamping mixing disk 6, is provided with beaker fixed groove in the fixed disk for mixing disk 5 of beaker
51。
Therein, the cabinet 1 and partition are an integral structure, and cabinet 1 and partition are the double-deck plate body structure, cabinet 1
With in the interlayer of partition be filled with mandruka.
Therein, the cushion blocking 11 and vibration damping noise control interlayer 102 are made of rubber material, and cabinet 1 is using stainless
Steel metal material is made.
One concrete application of the present apparatus are as follows: the beaker for being loaded with mother liquor sample is placed in the fixed mixing disk 5 of beaker
In beaker fixed groove 51, attaching plug 12 is accessed into alternating current and opens motor switch button 13, regulates motor frequency modulation
Knob 14；Mother liquor in beaker closes motor switch button 13 after mixing 15min, by mother liquor peace as requested be configured to 2 λ, λ,
The endotoxin standard solution of tetra- concentration of 0.5 λ and 0.25 λ, will be loaded with the test tube of the endotoxin standard solution of various concentration in 4
It is individually fixed in test tube clamping to mix in the test tube clamp groove 61 of disk 6, presses motor switch button 13, motor 2 is turning
In dynamic process, the driving gear 22 on main shaft 21 is with nutating gear 32 with the rotation of identical angular speed, transmission gear 32
The drive shaft 3 of upper fixation drives the test tube clamping being fixed thereon to mix the rotation of 6 same frequency of disk, solute and solvent in test tube
Vortex, which is generated, in rotation achievees the effect that mixing；Test tube clamping mixes disk 6 and is equipped with upper layer and lower layer, and every batch of can be handled more
Sample, reduces the workload of testing staff, the sample standard deviation of the various concentration of every batch of processing be in identical revolving speed and frequency into
Row mixes, and guarantees the consistency of the treatment conditions of different samples；Motor is fixed on vibration damping noise control interlayer 102 made of rubber
On, vibration damping noise control interlayer 102 can effectively absorb the vibrational energy generated when motor high-speed rotation, to reach vibration insulation and noise reduction
Effect is equipped with mandruka in the interlayer of cabinet, further decreases the noise generated during motor 2 rotates, ensure that
For a long time, the physical and mental health of the testing staff of long campaigns detection work；The bottom of cabinet is fixed with cushion blocking made of rubber
11, which can not only increase the frictional force with workbench, can also prevent cabinet occur in vibration processes it is mobile and
The problem of leading to deflection of test tube, while absorbing cabinet and occurring to vibrate and produce between workbench when motor 2 runs at high speed
Raw noise realizes that entire blending process carries out under the conditions of quietly.
In the description of this specification, the description of reference term " one embodiment ", " example ", " specific example " etc. means
Particular features, structures, materials, or characteristics described in conjunction with this embodiment or example are contained at least one implementation of the invention
In example or example.In the present specification, schematic expression of the above terms may not refer to the same embodiment or example.
Moreover, particular features, structures, materials, or characteristics described can be in any one or more of the embodiments or examples to close
Suitable mode combines.
Present invention disclosed above preferred embodiment is only intended to help to illustrate the present invention.There is no detailed for preferred embodiment
All details are described, are not limited the invention to the specific embodiments described.Obviously, according to the content of this specification,
It can make many modifications and variations.These embodiments are chosen and specifically described to this specification, is in order to better explain the present invention
Principle and practical application, so that skilled artisan be enable to better understand and utilize the present invention.The present invention is only
It is limited by claims and its full scope and equivalent.
Claims (4)
1. a kind of vortex evenly mixing device for microbial endotoxins detection, which is characterized in that including cabinet, box house is fixed
There is motor, motor clamps mixing disk by transmission mechanism with test tube respectively and the fixed disk that mixes of beaker is connect；
The shell of the cabinet is equipped with motor switch button and motor fm dial, and the side of cabinet is inserted equipped with power supply
The bottom insertion of head, cabinet is fixed with step-like cushion blocking, fixed backing plate is welded on box house bottom plate, motor passes through ground
Foot bolt is fixed on fixed backing plate, is equipped with vibration damping noise control interlayer, the top of motor and case between motor and fixed backing plate
It is equipped with partition between the top plate of body, is equipped with interlayer cavity between partition and top, the main shaft of motor is from partition and top plate
It is stretched out at center, driving gear is fixed on the main shaft in interlayer cavity, the side of main shaft is equipped with transmission gear, driving cog
The radius of wheel and the radius of driving gear are equal, and driving gear and transmission gear are intermeshed, and transmission gear passes through drive shaft
It is fixed in interlayer cavity, one end of drive shaft is fixed on the thrust ball bearing on partition and the other end is from the top plate of cabinet
Upper stretching；
The extension of the main shaft and drive shaft is equipped with limit stop ring, and beaker is fixed to mix disk and test tube clamping mixing disk
It is individually fixed in above the limit stop ring of main shaft and drive shaft, test tube clamping mixes disk and is equipped with two layers, the test tube clamp of lower layer
It holds to mix and is fixed with height adjustment column on disk, the test tube clamping on upper layer mixes disk and is fixed on height adjustment column.
2. a kind of vortex evenly mixing device for microbial endotoxins detection according to claim 1, which is characterized in that institute
The drive shaft stated is equipped with 4 and is uniformly distributed within one week around main shaft, and the test tube clamping of the fixed mixing disk of beaker and lower layer is mixed
Even disk is threadedly coupled with main shaft and drive shaft respectively, and the test tube clamping of lower layer, which mixes, is provided with thread groove at disk center, high
Degree adjustable column mixes disk with the clamping of the test tube of lower layer and is threadedly coupled, and it is logical to be provided with screw thread at the test tube clamping mixing disk center on upper layer
Hole, the test tube clamping on upper layer mix disk and connect with height adjustment male screw, are provided with test tube clamp in the disk of test tube clamping mixing disk
Groove is held, is provided with beaker fixed groove in the fixed disk for mixing disk of beaker.
3. a kind of vortex evenly mixing device for microbial endotoxins detection according to claim 1, which is characterized in that institute
The cabinet and partition stated are an integral structure, and cabinet and partition are the double-deck plate body structure, are filled in the interlayer of cabinet and partition
Mandruka.
4. a kind of vortex evenly mixing device for microbial endotoxins detection according to claim 1, which is characterized in that institute
The cushion blocking and vibration damping noise control interlayer stated are made of rubber material, and cabinet is made of stainless steel metal material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810708548.XA CN109030165B (en) | 2018-07-02 | 2018-07-02 | Vortex mixing device for detecting microbial endotoxin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810708548.XA CN109030165B (en) | 2018-07-02 | 2018-07-02 | Vortex mixing device for detecting microbial endotoxin |
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| Publication Number | Publication Date |
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| CN109030165A true CN109030165A (en) | 2018-12-18 |
| CN109030165B CN109030165B (en) | 2020-12-29 |
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| CN201810708548.XA Active CN109030165B (en) | 2018-07-02 | 2018-07-02 | Vortex mixing device for detecting microbial endotoxin |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114310524A (en) * | 2022-01-05 | 2022-04-12 | 徐州柏通铝业有限公司 | Double-sided grinding device for aluminum processing |
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