CN109030146A - A kind of sectioning cells reagent, kit and application method - Google Patents

A kind of sectioning cells reagent, kit and application method Download PDF

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Publication number
CN109030146A
CN109030146A CN201810823508.XA CN201810823508A CN109030146A CN 109030146 A CN109030146 A CN 109030146A CN 201810823508 A CN201810823508 A CN 201810823508A CN 109030146 A CN109030146 A CN 109030146A
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CN
China
Prior art keywords
reagent
glass slide
tube body
sectioning cells
cavity
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201810823508.XA
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Chinese (zh)
Inventor
黄荣祥
朱晨雁
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mike Audi (xiamen) Medical Diagnosis System Co Ltd
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Mike Audi (xiamen) Medical Diagnosis System Co Ltd
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Priority to CN201810823508.XA priority Critical patent/CN109030146A/en
Publication of CN109030146A publication Critical patent/CN109030146A/en
Pending legal-status Critical Current

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813Producing thin layers of samples on a substrate, e.g. smearing, spinning-on

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A kind of sectioning cells kit, it include: the reagent shell, glass slide and mobile fixed plate that can store reagent, the reagent shell includes the tube body for accommodating reagent, accommodates the cavity of glass slide and mobile fixed plate and increase the sealing ring of leakproofness between cavity and glass slide, after the glass slide is packed into cavity, mobile fixed plate is packed into cavity to fix glass slide.Sample processing reagent is loaded in advance in reagent storage portion in the present invention, sample processing reagent is consistent with client's test demand, it is characterized in that being combined into the sectioning cells kit for integrating transport, preservation, processing, client is facilitated directly to take, sample contamination risk is effectively reduced, saves social resources, effectively removes the impurity component in sample, it avoids interfering, significantly improves the diagosis quality of Pathology Doctors '.

Description

A kind of sectioning cells reagent, kit and application method
Technical field
The present invention relates to one kind to belong to pathological examination instrument and reagent, refers in particular to a kind of sectioning cells reagent, cell system Piece kit and application method.
Background technique
It is basic in many biology and medical application from the ingredient for having diagnostic significance is separated and purified in clinical sample Method, it be widely used in include cervical cytological examination, Patients Following Bone Marrowtransplantation virus infection the fields such as early diagnosis.It Not only first step of sample process and a vital step, because the confidence level of follow-up diagnosis result is largely Upper validity and purity depending on separated component.
Isopycnic sedimentation (isopycnic sedimentation) is used for partition density not equal particle.Cell or cell Device through sufficiently large centrifugal force and enough long-times, is settled or is floated at the medium equal with autologous density, and stopped in the medium It stays in there and reaches balance, thus by the cell of different densities or organelle separation and capture.The sample of list marketing at present is close Degree separating liquid has very much, there is foreign brand name, also has domestic brand, is mainly loaded in reagent bottle with the specification of 500ml in packaging, visitor It when in use, takes on demand at family.However it frequently takes and occupies a large amount of quality time, while also adding product dirt behind Kaifeng The probability of dye.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of sectioning cells kit device and application methods, by container and examination Agent is combined into the sample processing reagent box for integrating transport, preservation, processing, facilitates client directly to take, sample is effectively reduced Pollution risk effectively removes the impurity component in sample, avoids interfering, significantly improve the diagosis quality of Pathology Doctors '.
In order to solve the above-mentioned technical problem, the technical solution of the present invention is as follows: a kind of sectioning cells kit, feature exist In, comprising: one can store the reagent shell, glass slide and mobile fixed plate of reagent, and the reagent shell includes accommodating reagent Tube body, accommodate the cavity of glass slide and mobile fixed plate and increase the sealing ring of leakproofness between cavity and glass slide, it is described Glass slide be packed into cavity after, mobile fixed plate is packed into cavity to fix glass slide.
Further, a conical flow guiding part is equipped in the tube body, the bottom of the conical flow guiding part is equipped with several Deflector hole, the reagent flow to bottom along inboard wall of tube body by deflector hole.
Further, the angle between the tapered surface and inboard wall of tube body of the conical flow guiding part is A, 45 ° >=A >=30 °.
Further, a sealing cover is also equipped on the tube body, the sealing is placed on tube body and cooperatively forms a receiving The seal cavity of reagent.
Further, the junction of tube body and cavity is arranged in the sealing ring.
A kind of sectioning cells reagent, which is characterized in that the reagent is by ficoll, glycerine, P-hydroxybenzoic acid second Ester repairs liquid, adhesion promoter and ultrapure water composition.
Further, in reagent storage sectioning cells kit described in claim 1-5.
Further, the component proportion of the reagent are as follows: the concentration of ficoll is 1%~20%, and glycerol concentration is 5%~40%, ethyl-para-hydroxybenzoate concentration is 0.1%-0.5%, and reparation liquid concentration is 0.1%-1%, and adhesive force promotes Agent, concentration 0.1%-1%.
Further, the reparation liquid is by trishydroxymethylaminomethane (Tris), polyoxyethylene sorbitan Dan Yue One kind or two kinds of cinnamic acid ester (Tween-20), disodium ethylene diamine tetraacetate, citric acid composition.
Further, the adhesion promoter uses poly-D-lysine or amino silicone.
Further, the ficoll uses ficoll 400.
A kind of application method of sectioning cells kit, which comprises the following steps:
Step 1: removing sealing cover, sample is added, and sealing cover is fixed on tube body again;
Step 2: kit is placed on cell smear centrifuge;
Step 3: turning centrifugation 2-3 minutes on revolving speed 1100-1500r/min centrifuge, abandon supernatant;
Step 4: removing glass slide, be placed in 95% ethyl alcohol fixed.
Advantages and advantages of the invention are:
1, new manner of packing can be more convenient guest operation, and the wind of cross contamination in sample centrifugal process is effectively reduced Danger;
2, disposable, pollution caused by avoiding big specification bottling liquid from frequently uncapping;
3, hermetically sealed packaging, can high-temperature sterilization disinfection, meet client to the sterility requirements of product;
4, product composition is few, substitutes cardiografin using glycerine, effectively reduces cost, improves sample process quality;
5, integrate transport, preservation, processing, it is convenient and practical;
6, it can show cell that antigen carries out appropriate reparation while enrichment of cell, restore antigen three-dimensional structure, and Improve adhesiveness;
7, adhesion promoter, which has, pre-processes glass slide while preservation, transport sample reagent treatment, Glass slide adhesiveness is maintained, avoids glass slide long period of soaking liquid so that adhesion failure;
8, for the first time using disjunctor pyramidal structure built in the tube body of granular tableting agents box, shaped like bamboo hat, when operator is directly by sample When being originally quickly poured into the tubing string of film-making device, using pyramidal structure as buffering, sample is avoided directly to impact sample processing reagent, broken The layering of bad reagent, reduces concentration effect;
9, centrifugal sedimentation is combined into one, and it is convenient to operate.
Detailed description of the invention
Specific embodiments of the present invention will be described in further detail with reference to the accompanying drawing.
Fig. 1 is Structure explosion diagram of the invention;
Fig. 2 is cross-sectional view of the invention;
Fig. 3 is one effect picture of the embodiment of the present invention;
Fig. 4 is two effect picture of the embodiment of the present invention;
Fig. 5 is three effect picture of the embodiment of the present invention.
Specific embodiment
With reference to the accompanying drawings and detailed description, the present invention will be further described.
As shown in Figure 1, 2, a kind of sectioning cells kit, comprising: one can store the reagent shell 10 of reagent, glass slide 20 With mobile fixed plate 30, the reagent shell 10 includes the tube body 11 for accommodating reagent, accommodates glass slide 20 and mobile fixed plate Cavity 12 and increase the sealing ring 13 of leakproofness between cavity 12 and glass slide 20, sealing ring 13 is arranged in tube body 11 and cavity 12 junction, is equipped with a conical flow guiding part 40 in tube body 11, between 11 inner wall of tapered surface and tube body of conical flow guiding part 40 Angle is A, and 45 ° >=A >=30 °, the bottom of conical flow guiding part 40 is equipped with several deflector holes 41, and the reagent passes through deflector hole 41 The bottom of tube body 11 is flow to along the inner wall of tube body 11.After the glass slide 20 is packed into cavity 12, mobile fixed plate 30 is packed into Cavity 12 is to fix glass slide 20.A sealing cover 50 is also equipped on tube body, the sealing cover 50 cooperatively forms one in tube body 11 Accommodate the seal cavity of reagent.
Reagent is stored in the tube body 11, by ficoll, glycerine, ethyl-para-hydroxybenzoate, repairs liquid, adhesive force promotion Agent and ultrapure water composition.The concentration of ficoll is 1%~20%, and glycerol concentration is 5%~40%, ethyl-para-hydroxybenzoate Concentration is 0.1%-0.5%, and reparation liquid concentration is 0.1%-1%, adhesion promoter, concentration 0.1%-1%.Repair liquid by Trishydroxymethylaminomethane (Tris), polyoxyethylene 20 sorbitan monolaurate (Tween-20), ethylenediamine tetra-acetic acid two One kind or two kinds of sodium, citric acid composition.Adhesion promoter uses poly-D-lysine or amino silicone.Preferably, institute The ficoll stated uses ficoll 400.
The reagent of the sample process is loaded in advance in sectioning cells kit, and sample processing reagent and client test demand Amount is consistent, is combined into the sectioning cells kit for integrating transport, preservation, processing.
Embodiment one:
It is configured to 100g sample processing reagent by said components, mentioned component is dissolved in ultrapure water, is stirred for mixing, is surpassed Pure water supplies 100g.
Packing process: taking 10ml quantitative liquid-distributor, by 2ml sample processing reagent quantitative separating in sectioning cells kit Tube body in, and sealed with sealing cover body, 5 are one group, and plastic packaging is made into merchandise sales in vacuum formed box.
Application method:
Step 1: removing sealing cover body, sample is added, and sealing cover body is fixed on reagent storage portion tube body again;
Step 2: kit is placed on cell smear centrifuge;
Step 3: turning centrifugation 2 minutes on 1500 revolving speed of centrifuge, abandon supernatant;
Step 4: removing glass slide, be placed in 95% ethyl alcohol fixed.
Film-making, row pap staining, effect corresponding diagram 3 after the dyeing of embodiment one are completed according to above-mentioned steps.
Embodiment two:
It is configured to 100g sample processing reagent by said components, mentioned component is dissolved in ultrapure water, is stirred for mixing, is surpassed Pure water supplies 100g.
Packing process: taking 10ml quantitative liquid-distributor, by 2ml sample processing reagent quantitative separating in sectioning cells kit Tube body in, and sealed with sealing cover body, 10 are one group, and plastic packaging is made into merchandise sales in vacuum formed box.
Application method:
Step 1: removing sealing cover body, sample is added, and sealing cover body is fixed on reagent storage portion tube body again;
Step 2: kit is placed on cell smear centrifuge;
Step 3: turning centrifugation 2 minutes on 1100 revolving speed of centrifuge, abandon supernatant;
Step 4: removing glass slide, be placed in 95% ethyl alcohol fixed.
Film-making, row pap staining, effect corresponding diagram 4 after the dyeing of embodiment two are completed according to above-mentioned steps.
Embodiment three:
Component Weight
Ficoll 400 5.00g
Glycerine 40.00g
Ethyl-para-hydroxybenzoate 0.50g
Disodium ethylene diamine tetraacetate 0.25g
Amino silicone 0.50g
Ultrapure water Supply 100g
It is configured to 100g sample processing reagent by said components, mentioned component is dissolved in ultrapure water, is stirred for mixing, is surpassed Pure water supplies 100g.
Packing process: taking 10ml quantitative liquid-distributor, by 2ml sample processing reagent quantitative separating in the tube body of film-making device It is interior, and sealed with sealing cover body, 25 are one group, and plastic packaging is made into merchandise sales in vacuum formed box.
Application method:
Step 1: removing sealing cover body, sample is added, and sealing cover body is fixed on reagent storage portion tube body again;
Step 2: kit is placed on cell smear centrifuge;
Step 3: turning centrifugation 3 minutes on 1100 revolving speed of centrifuge, abandon supernatant;
Step 4: removing glass slide, be placed in 95% ethyl alcohol fixed.
Film-making, row pap staining, effect corresponding diagram 5 after the dyeing of embodiment three are completed according to above-mentioned steps.
Although specifically showing and describing the present invention in conjunction with preferred embodiment, those skilled in the art should be bright It is white, it is not departing from the spirit and scope of the present invention defined by the appended claims, in the form and details to this hair It is bright to make a variety of changes, it is protection scope of the present invention.

Claims (12)

1. a kind of sectioning cells kit characterized by comprising one can store reagent shell, glass slide and the movement of reagent Fixed plate, the reagent shell include the tube body for accommodating reagent, accommodate the cavity of glass slide and mobile fixed plate and increase chamber The sealing ring of leakproofness between body and glass slide, after the glass slide is packed into cavity, mobile fixed plate is packed into cavity with fixation Glass slide.
2. a kind of sectioning cells kit according to claim 1, which is characterized in that be equipped with a taper in the tube body Conducting element, the bottom of the conical flow guiding part are equipped with several deflector holes, and the reagent is by deflector hole along inboard wall of tube body It flow to bottom.
3. a kind of sectioning cells kit according to claim 2, which is characterized in that the taper of the conical flow guiding part Angle between face and inboard wall of tube body is A, 45 ° >=A >=30 °.
4. a kind of sectioning cells kit according to claim 1, which is characterized in that be also equipped with one on the tube body Sealing cover, the sealing are placed on the seal cavity that tube body cooperatively forms a receiving reagent.
5. a kind of sectioning cells kit according to claim 1, which is characterized in that the sealing ring is arranged in tube body With the junction of cavity.
6. a kind of sectioning cells reagent, which is characterized in that the reagent by ficoll, glycerine, ethyl-para-hydroxybenzoate, Repair liquid, adhesion promoter and ultrapure water composition.
7. a kind of sectioning cells granular tableting agents according to claim 6, which is characterized in that the reagent storage is in right It is required that in sectioning cells kit described in 1-5.
8. a kind of sectioning cells granular tableting agents according to claim 6, which is characterized in that the component proportion of the reagent Are as follows: the concentration of ficoll is 1%~20%, and glycerol concentration is 5%~40%, and ethyl-para-hydroxybenzoate concentration is 0.1%- 0.5%, reparation liquid concentration is 0.1%-1%, adhesion promoter, concentration 0.1%-1%.
9. a kind of sectioning cells granular tableting agents according to claim 6, which is characterized in that the reparation liquid is by three hydroxyl first Base aminomethane (Tris), polyoxyethylene 20 sorbitan monolaurate (Tween-20), disodium ethylene diamine tetraacetate, lemon One kind or two kinds of acid composition.
10. a kind of sectioning cells granular tableting agents according to claim 6, which is characterized in that the adhesion promoter Using poly-D-lysine or amino silicone.
11. a kind of sectioning cells granular tableting agents according to claim 6, which is characterized in that the ficoll is using poly- Sucrose 400.
12. a kind of application method of sectioning cells kit, which comprises the following steps:
Step 1: removing sealing cover, sample is added, and sealing cover is fixed on tube body again;
Step 2: kit is placed on cell smear centrifuge;
Step 3: turning centrifugation 2-3 minutes on revolving speed 1100-1500r/min centrifuge, abandon supernatant;
Step 4: removing glass slide, be placed in 95% ethyl alcohol fixed.
CN201810823508.XA 2018-07-25 2018-07-25 A kind of sectioning cells reagent, kit and application method Pending CN109030146A (en)

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Application Number Priority Date Filing Date Title
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111811901A (en) * 2020-06-30 2020-10-23 郑志森 Cell block collecting device and cell block collecting method

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US5840502A (en) * 1994-08-31 1998-11-24 Activated Cell Therapy, Inc. Methods for enriching specific cell-types by density gradient centrifugation
CN1967193A (en) * 2005-11-18 2007-05-23 上海昂佰生物医学科技有限公司 Cell processing kit and using method therefor
CN101246096A (en) * 2008-03-12 2008-08-20 郑明� Centrifugal pellet mill and hanging cradle used for the same
CN202471474U (en) * 2011-12-02 2012-10-03 孝感奥华医疗科技有限公司 Slide making clamp for cell slide maker
US20140087360A1 (en) * 2011-05-05 2014-03-27 Stemcell Technologies, Inc. Method and insert for density gradient separation
CN203606191U (en) * 2013-11-26 2014-05-21 上海宇度医学科技股份有限公司 Thin-layer liquid-based cell slide maker
CN205593811U (en) * 2016-03-11 2016-09-21 厦门信道生物技术有限公司 Concentrated smear device
CN106047795A (en) * 2016-06-22 2016-10-26 杭州海世嘉生物科技有限公司 Sample density separation liquid and preparation method thereof
CN205719721U (en) * 2016-06-25 2016-11-23 南京健邦锦源医疗仪器有限公司 A kind of Thinprep pap test film-making filter membrane cylinder

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5840502A (en) * 1994-08-31 1998-11-24 Activated Cell Therapy, Inc. Methods for enriching specific cell-types by density gradient centrifugation
CN1967193A (en) * 2005-11-18 2007-05-23 上海昂佰生物医学科技有限公司 Cell processing kit and using method therefor
CN101246096A (en) * 2008-03-12 2008-08-20 郑明� Centrifugal pellet mill and hanging cradle used for the same
US20140087360A1 (en) * 2011-05-05 2014-03-27 Stemcell Technologies, Inc. Method and insert for density gradient separation
CN202471474U (en) * 2011-12-02 2012-10-03 孝感奥华医疗科技有限公司 Slide making clamp for cell slide maker
CN203606191U (en) * 2013-11-26 2014-05-21 上海宇度医学科技股份有限公司 Thin-layer liquid-based cell slide maker
CN205593811U (en) * 2016-03-11 2016-09-21 厦门信道生物技术有限公司 Concentrated smear device
CN106047795A (en) * 2016-06-22 2016-10-26 杭州海世嘉生物科技有限公司 Sample density separation liquid and preparation method thereof
CN205719721U (en) * 2016-06-25 2016-11-23 南京健邦锦源医疗仪器有限公司 A kind of Thinprep pap test film-making filter membrane cylinder

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111811901A (en) * 2020-06-30 2020-10-23 郑志森 Cell block collecting device and cell block collecting method
CN111811901B (en) * 2020-06-30 2022-09-20 郑志森 Cell block collecting device and cell block collecting method

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Application publication date: 20181218