CN109030146A - A kind of sectioning cells reagent, kit and application method - Google Patents
A kind of sectioning cells reagent, kit and application method Download PDFInfo
- Publication number
- CN109030146A CN109030146A CN201810823508.XA CN201810823508A CN109030146A CN 109030146 A CN109030146 A CN 109030146A CN 201810823508 A CN201810823508 A CN 201810823508A CN 109030146 A CN109030146 A CN 109030146A
- Authority
- CN
- China
- Prior art keywords
- reagent
- glass slide
- tube body
- sectioning cells
- cavity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 54
- 238000000034 method Methods 0.000 title claims description 19
- 239000011521 glass Substances 0.000 claims abstract description 28
- 238000007789 sealing Methods 0.000 claims abstract description 26
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 19
- 229920001917 Ficoll Polymers 0.000 claims description 12
- NUVBSKCKDOMJSU-UHFFFAOYSA-N ethylparaben Chemical compound CCOC(=O)C1=CC=C(O)C=C1 NUVBSKCKDOMJSU-UHFFFAOYSA-N 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 10
- 239000003795 chemical substances by application Substances 0.000 claims description 9
- 239000002318 adhesion promoter Substances 0.000 claims description 8
- 235000011187 glycerol Nutrition 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 7
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 7
- 239000012498 ultrapure water Substances 0.000 claims description 7
- 235000010228 ethyl p-hydroxybenzoate Nutrition 0.000 claims description 6
- 239000004403 ethyl p-hydroxybenzoate Substances 0.000 claims description 6
- 229920001213 Polysorbate 20 Polymers 0.000 claims description 5
- 238000005119 centrifugation Methods 0.000 claims description 5
- 235000019441 ethanol Nutrition 0.000 claims description 5
- 239000006228 supernatant Substances 0.000 claims description 5
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 5
- 229920013822 aminosilicone Polymers 0.000 claims description 4
- 230000008439 repair process Effects 0.000 claims description 4
- KDXKERNSBIXSRK-RXMQYKEDSA-N D-lysine Chemical compound NCCCC[C@@H](N)C(O)=O KDXKERNSBIXSRK-RXMQYKEDSA-N 0.000 claims description 3
- 239000003109 Disodium ethylene diamine tetraacetate Substances 0.000 claims description 3
- 239000007983 Tris buffer Substances 0.000 claims description 3
- 235000019301 disodium ethylene diamine tetraacetate Nutrition 0.000 claims description 3
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims description 3
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims description 3
- 235000005979 Citrus limon Nutrition 0.000 claims 1
- 244000248349 Citrus limon Species 0.000 claims 1
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 claims 1
- BAVYZALUXZFZLV-UHFFFAOYSA-N Methylamine Chemical compound NC BAVYZALUXZFZLV-UHFFFAOYSA-N 0.000 claims 1
- 229930006000 Sucrose Natural products 0.000 claims 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims 1
- 239000005720 sucrose Substances 0.000 claims 1
- 238000004321 preservation Methods 0.000 abstract description 5
- 239000012535 impurity Substances 0.000 abstract description 2
- 230000002452 interceptive effect Effects 0.000 abstract description 2
- 230000007170 pathology Effects 0.000 abstract description 2
- 238000011109 contamination Methods 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 22
- 230000000694 effects Effects 0.000 description 7
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 6
- 238000004806 packaging method and process Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 238000012856 packing Methods 0.000 description 4
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 3
- 238000004043 dyeing Methods 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 238000004062 sedimentation Methods 0.000 description 3
- 238000010186 staining Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- FJKROLUGYXJWQN-UHFFFAOYSA-N 4-hydroxybenzoic acid Chemical compound OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 2
- 239000000853 adhesive Substances 0.000 description 2
- 230000001070 adhesive effect Effects 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- WBYWAXJHAXSJNI-VOTSOKGWSA-M .beta-Phenylacrylic acid Natural products [O-]C(=O)\C=C\C1=CC=CC=C1 WBYWAXJHAXSJNI-VOTSOKGWSA-M 0.000 description 1
- 229940090248 4-hydroxybenzoic acid Drugs 0.000 description 1
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 1
- 235000017491 Bambusa tulda Nutrition 0.000 description 1
- 241001330002 Bambuseae Species 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 1
- 229920001214 Polysorbate 60 Polymers 0.000 description 1
- 239000011425 bamboo Substances 0.000 description 1
- 238000010322 bone marrow transplantation Methods 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 229930016911 cinnamic acid Natural products 0.000 description 1
- 235000013985 cinnamic acid Nutrition 0.000 description 1
- -1 cinnamic acid ester Chemical class 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 230000002380 cytological effect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000013399 early diagnosis Methods 0.000 description 1
- 229960001484 edetic acid Drugs 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 238000004880 explosion Methods 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- MIKKOBKEXMRYFQ-WZTVWXICSA-N meglumine amidotrizoate Chemical compound C[NH2+]C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO.CC(=O)NC1=C(I)C(NC(C)=O)=C(I)C(C([O-])=O)=C1I MIKKOBKEXMRYFQ-WZTVWXICSA-N 0.000 description 1
- WBYWAXJHAXSJNI-UHFFFAOYSA-N methyl p-hydroxycinnamate Natural products OC(=O)C=CC1=CC=CC=C1 WBYWAXJHAXSJNI-UHFFFAOYSA-N 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
A kind of sectioning cells kit, it include: the reagent shell, glass slide and mobile fixed plate that can store reagent, the reagent shell includes the tube body for accommodating reagent, accommodates the cavity of glass slide and mobile fixed plate and increase the sealing ring of leakproofness between cavity and glass slide, after the glass slide is packed into cavity, mobile fixed plate is packed into cavity to fix glass slide.Sample processing reagent is loaded in advance in reagent storage portion in the present invention, sample processing reagent is consistent with client's test demand, it is characterized in that being combined into the sectioning cells kit for integrating transport, preservation, processing, client is facilitated directly to take, sample contamination risk is effectively reduced, saves social resources, effectively removes the impurity component in sample, it avoids interfering, significantly improves the diagosis quality of Pathology Doctors '.
Description
Technical field
The present invention relates to one kind to belong to pathological examination instrument and reagent, refers in particular to a kind of sectioning cells reagent, cell system
Piece kit and application method.
Background technique
It is basic in many biology and medical application from the ingredient for having diagnostic significance is separated and purified in clinical sample
Method, it be widely used in include cervical cytological examination, Patients Following Bone Marrowtransplantation virus infection the fields such as early diagnosis.It
Not only first step of sample process and a vital step, because the confidence level of follow-up diagnosis result is largely
Upper validity and purity depending on separated component.
Isopycnic sedimentation (isopycnic sedimentation) is used for partition density not equal particle.Cell or cell
Device through sufficiently large centrifugal force and enough long-times, is settled or is floated at the medium equal with autologous density, and stopped in the medium
It stays in there and reaches balance, thus by the cell of different densities or organelle separation and capture.The sample of list marketing at present is close
Degree separating liquid has very much, there is foreign brand name, also has domestic brand, is mainly loaded in reagent bottle with the specification of 500ml in packaging, visitor
It when in use, takes on demand at family.However it frequently takes and occupies a large amount of quality time, while also adding product dirt behind Kaifeng
The probability of dye.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of sectioning cells kit device and application methods, by container and examination
Agent is combined into the sample processing reagent box for integrating transport, preservation, processing, facilitates client directly to take, sample is effectively reduced
Pollution risk effectively removes the impurity component in sample, avoids interfering, significantly improve the diagosis quality of Pathology Doctors '.
In order to solve the above-mentioned technical problem, the technical solution of the present invention is as follows: a kind of sectioning cells kit, feature exist
In, comprising: one can store the reagent shell, glass slide and mobile fixed plate of reagent, and the reagent shell includes accommodating reagent
Tube body, accommodate the cavity of glass slide and mobile fixed plate and increase the sealing ring of leakproofness between cavity and glass slide, it is described
Glass slide be packed into cavity after, mobile fixed plate is packed into cavity to fix glass slide.
Further, a conical flow guiding part is equipped in the tube body, the bottom of the conical flow guiding part is equipped with several
Deflector hole, the reagent flow to bottom along inboard wall of tube body by deflector hole.
Further, the angle between the tapered surface and inboard wall of tube body of the conical flow guiding part is A, 45 ° >=A >=30 °.
Further, a sealing cover is also equipped on the tube body, the sealing is placed on tube body and cooperatively forms a receiving
The seal cavity of reagent.
Further, the junction of tube body and cavity is arranged in the sealing ring.
A kind of sectioning cells reagent, which is characterized in that the reagent is by ficoll, glycerine, P-hydroxybenzoic acid second
Ester repairs liquid, adhesion promoter and ultrapure water composition.
Further, in reagent storage sectioning cells kit described in claim 1-5.
Further, the component proportion of the reagent are as follows: the concentration of ficoll is 1%~20%, and glycerol concentration is
5%~40%, ethyl-para-hydroxybenzoate concentration is 0.1%-0.5%, and reparation liquid concentration is 0.1%-1%, and adhesive force promotes
Agent, concentration 0.1%-1%.
Further, the reparation liquid is by trishydroxymethylaminomethane (Tris), polyoxyethylene sorbitan Dan Yue
One kind or two kinds of cinnamic acid ester (Tween-20), disodium ethylene diamine tetraacetate, citric acid composition.
Further, the adhesion promoter uses poly-D-lysine or amino silicone.
Further, the ficoll uses ficoll 400.
A kind of application method of sectioning cells kit, which comprises the following steps:
Step 1: removing sealing cover, sample is added, and sealing cover is fixed on tube body again；
Step 2: kit is placed on cell smear centrifuge；
Step 3: turning centrifugation 2-3 minutes on revolving speed 1100-1500r/min centrifuge, abandon supernatant；
Step 4: removing glass slide, be placed in 95% ethyl alcohol fixed.
Advantages and advantages of the invention are:
1, new manner of packing can be more convenient guest operation, and the wind of cross contamination in sample centrifugal process is effectively reduced
Danger；
2, disposable, pollution caused by avoiding big specification bottling liquid from frequently uncapping；
3, hermetically sealed packaging, can high-temperature sterilization disinfection, meet client to the sterility requirements of product；
4, product composition is few, substitutes cardiografin using glycerine, effectively reduces cost, improves sample process quality；
5, integrate transport, preservation, processing, it is convenient and practical；
6, it can show cell that antigen carries out appropriate reparation while enrichment of cell, restore antigen three-dimensional structure, and
Improve adhesiveness；
7, adhesion promoter, which has, pre-processes glass slide while preservation, transport sample reagent treatment,
Glass slide adhesiveness is maintained, avoids glass slide long period of soaking liquid so that adhesion failure；
8, for the first time using disjunctor pyramidal structure built in the tube body of granular tableting agents box, shaped like bamboo hat, when operator is directly by sample
When being originally quickly poured into the tubing string of film-making device, using pyramidal structure as buffering, sample is avoided directly to impact sample processing reagent, broken
The layering of bad reagent, reduces concentration effect；
9, centrifugal sedimentation is combined into one, and it is convenient to operate.
Detailed description of the invention
Specific embodiments of the present invention will be described in further detail with reference to the accompanying drawing.
Fig. 1 is Structure explosion diagram of the invention；
Fig. 2 is cross-sectional view of the invention；
Fig. 3 is one effect picture of the embodiment of the present invention；
Fig. 4 is two effect picture of the embodiment of the present invention；
Fig. 5 is three effect picture of the embodiment of the present invention.
Specific embodiment
With reference to the accompanying drawings and detailed description, the present invention will be further described.
As shown in Figure 1, 2, a kind of sectioning cells kit, comprising: one can store the reagent shell 10 of reagent, glass slide 20
With mobile fixed plate 30, the reagent shell 10 includes the tube body 11 for accommodating reagent, accommodates glass slide 20 and mobile fixed plate
Cavity 12 and increase the sealing ring 13 of leakproofness between cavity 12 and glass slide 20, sealing ring 13 is arranged in tube body 11 and cavity
12 junction, is equipped with a conical flow guiding part 40 in tube body 11, between 11 inner wall of tapered surface and tube body of conical flow guiding part 40
Angle is A, and 45 ° >=A >=30 °, the bottom of conical flow guiding part 40 is equipped with several deflector holes 41, and the reagent passes through deflector hole 41
The bottom of tube body 11 is flow to along the inner wall of tube body 11.After the glass slide 20 is packed into cavity 12, mobile fixed plate 30 is packed into
Cavity 12 is to fix glass slide 20.A sealing cover 50 is also equipped on tube body, the sealing cover 50 cooperatively forms one in tube body 11
Accommodate the seal cavity of reagent.
Reagent is stored in the tube body 11, by ficoll, glycerine, ethyl-para-hydroxybenzoate, repairs liquid, adhesive force promotion
Agent and ultrapure water composition.The concentration of ficoll is 1%~20%, and glycerol concentration is 5%~40%, ethyl-para-hydroxybenzoate
Concentration is 0.1%-0.5%, and reparation liquid concentration is 0.1%-1%, adhesion promoter, concentration 0.1%-1%.Repair liquid by
Trishydroxymethylaminomethane (Tris), polyoxyethylene 20 sorbitan monolaurate (Tween-20), ethylenediamine tetra-acetic acid two
One kind or two kinds of sodium, citric acid composition.Adhesion promoter uses poly-D-lysine or amino silicone.Preferably, institute
The ficoll stated uses ficoll 400.
The reagent of the sample process is loaded in advance in sectioning cells kit, and sample processing reagent and client test demand
Amount is consistent, is combined into the sectioning cells kit for integrating transport, preservation, processing.
Embodiment one:
It is configured to 100g sample processing reagent by said components, mentioned component is dissolved in ultrapure water, is stirred for mixing, is surpassed
Pure water supplies 100g.
Packing process: taking 10ml quantitative liquid-distributor, by 2ml sample processing reagent quantitative separating in sectioning cells kit
Tube body in, and sealed with sealing cover body, 5 are one group, and plastic packaging is made into merchandise sales in vacuum formed box.
Application method:
Step 1: removing sealing cover body, sample is added, and sealing cover body is fixed on reagent storage portion tube body again；
Step 2: kit is placed on cell smear centrifuge；
Step 3: turning centrifugation 2 minutes on 1500 revolving speed of centrifuge, abandon supernatant；
Step 4: removing glass slide, be placed in 95% ethyl alcohol fixed.
Film-making, row pap staining, effect corresponding diagram 3 after the dyeing of embodiment one are completed according to above-mentioned steps.
Embodiment two:
It is configured to 100g sample processing reagent by said components, mentioned component is dissolved in ultrapure water, is stirred for mixing, is surpassed
Pure water supplies 100g.
Packing process: taking 10ml quantitative liquid-distributor, by 2ml sample processing reagent quantitative separating in sectioning cells kit
Tube body in, and sealed with sealing cover body, 10 are one group, and plastic packaging is made into merchandise sales in vacuum formed box.
Application method:
Step 1: removing sealing cover body, sample is added, and sealing cover body is fixed on reagent storage portion tube body again；
Step 2: kit is placed on cell smear centrifuge；
Step 3: turning centrifugation 2 minutes on 1100 revolving speed of centrifuge, abandon supernatant；
Step 4: removing glass slide, be placed in 95% ethyl alcohol fixed.
Film-making, row pap staining, effect corresponding diagram 4 after the dyeing of embodiment two are completed according to above-mentioned steps.
Embodiment three:
| Component | Weight |
| Ficoll 400 | 5.00g |
| Glycerine | 40.00g |
| Ethyl-para-hydroxybenzoate | 0.50g |
| Disodium ethylene diamine tetraacetate | 0.25g |
| Amino silicone | 0.50g |
| Ultrapure water | Supply 100g |
It is configured to 100g sample processing reagent by said components, mentioned component is dissolved in ultrapure water, is stirred for mixing, is surpassed
Pure water supplies 100g.
Packing process: taking 10ml quantitative liquid-distributor, by 2ml sample processing reagent quantitative separating in the tube body of film-making device
It is interior, and sealed with sealing cover body, 25 are one group, and plastic packaging is made into merchandise sales in vacuum formed box.
Application method:
Step 1: removing sealing cover body, sample is added, and sealing cover body is fixed on reagent storage portion tube body again；
Step 2: kit is placed on cell smear centrifuge；
Step 3: turning centrifugation 3 minutes on 1100 revolving speed of centrifuge, abandon supernatant；
Step 4: removing glass slide, be placed in 95% ethyl alcohol fixed.
Film-making, row pap staining, effect corresponding diagram 5 after the dyeing of embodiment three are completed according to above-mentioned steps.
Although specifically showing and describing the present invention in conjunction with preferred embodiment, those skilled in the art should be bright
It is white, it is not departing from the spirit and scope of the present invention defined by the appended claims, in the form and details to this hair
It is bright to make a variety of changes, it is protection scope of the present invention.
Claims (12)
1. a kind of sectioning cells kit characterized by comprising one can store reagent shell, glass slide and the movement of reagent
Fixed plate, the reagent shell include the tube body for accommodating reagent, accommodate the cavity of glass slide and mobile fixed plate and increase chamber
The sealing ring of leakproofness between body and glass slide, after the glass slide is packed into cavity, mobile fixed plate is packed into cavity with fixation
Glass slide.
2. a kind of sectioning cells kit according to claim 1, which is characterized in that be equipped with a taper in the tube body
Conducting element, the bottom of the conical flow guiding part are equipped with several deflector holes, and the reagent is by deflector hole along inboard wall of tube body
It flow to bottom.
3. a kind of sectioning cells kit according to claim 2, which is characterized in that the taper of the conical flow guiding part
Angle between face and inboard wall of tube body is A, 45 ° >=A >=30 °.
4. a kind of sectioning cells kit according to claim 1, which is characterized in that be also equipped with one on the tube body
Sealing cover, the sealing are placed on the seal cavity that tube body cooperatively forms a receiving reagent.
5. a kind of sectioning cells kit according to claim 1, which is characterized in that the sealing ring is arranged in tube body
With the junction of cavity.
6. a kind of sectioning cells reagent, which is characterized in that the reagent by ficoll, glycerine, ethyl-para-hydroxybenzoate,
Repair liquid, adhesion promoter and ultrapure water composition.
7. a kind of sectioning cells granular tableting agents according to claim 6, which is characterized in that the reagent storage is in right
It is required that in sectioning cells kit described in 1-5.
8. a kind of sectioning cells granular tableting agents according to claim 6, which is characterized in that the component proportion of the reagent
Are as follows: the concentration of ficoll is 1%~20%, and glycerol concentration is 5%~40%, and ethyl-para-hydroxybenzoate concentration is 0.1%-
0.5%, reparation liquid concentration is 0.1%-1%, adhesion promoter, concentration 0.1%-1%.
9. a kind of sectioning cells granular tableting agents according to claim 6, which is characterized in that the reparation liquid is by three hydroxyl first
Base aminomethane (Tris), polyoxyethylene 20 sorbitan monolaurate (Tween-20), disodium ethylene diamine tetraacetate, lemon
One kind or two kinds of acid composition.
10. a kind of sectioning cells granular tableting agents according to claim 6, which is characterized in that the adhesion promoter
Using poly-D-lysine or amino silicone.
11. a kind of sectioning cells granular tableting agents according to claim 6, which is characterized in that the ficoll is using poly-
Sucrose 400.
12. a kind of application method of sectioning cells kit, which comprises the following steps:
Step 1: removing sealing cover, sample is added, and sealing cover is fixed on tube body again；
Step 2: kit is placed on cell smear centrifuge；
Step 3: turning centrifugation 2-3 minutes on revolving speed 1100-1500r/min centrifuge, abandon supernatant；
Step 4: removing glass slide, be placed in 95% ethyl alcohol fixed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810823508.XA CN109030146A (en) | 2018-07-25 | 2018-07-25 | A kind of sectioning cells reagent, kit and application method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810823508.XA CN109030146A (en) | 2018-07-25 | 2018-07-25 | A kind of sectioning cells reagent, kit and application method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109030146A true CN109030146A (en) | 2018-12-18 |
Family
ID=64644922
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810823508.XA Pending CN109030146A (en) | 2018-07-25 | 2018-07-25 | A kind of sectioning cells reagent, kit and application method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109030146A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111811901A (en) * | 2020-06-30 | 2020-10-23 | 郑志森 | Cell block collecting device and cell block collecting method |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5840502A (en) * | 1994-08-31 | 1998-11-24 | Activated Cell Therapy, Inc. | Methods for enriching specific cell-types by density gradient centrifugation |
| CN1967193A (en) * | 2005-11-18 | 2007-05-23 | 上海昂佰生物医学科技有限公司 | Cell processing kit and using method therefor |
| CN101246096A (en) * | 2008-03-12 | 2008-08-20 | 郑明� | Centrifugal pellet mill and hanging cradle used for the same |
| CN202471474U (en) * | 2011-12-02 | 2012-10-03 | 孝感奥华医疗科技有限公司 | Slide making clamp for cell slide maker |
| US20140087360A1 (en) * | 2011-05-05 | 2014-03-27 | Stemcell Technologies, Inc. | Method and insert for density gradient separation |
| CN203606191U (en) * | 2013-11-26 | 2014-05-21 | 上海宇度医学科技股份有限公司 | Thin-layer liquid-based cell slide maker |
| CN205593811U (en) * | 2016-03-11 | 2016-09-21 | 厦门信道生物技术有限公司 | Concentrated smear device |
| CN106047795A (en) * | 2016-06-22 | 2016-10-26 | 杭州海世嘉生物科技有限公司 | Sample density separation liquid and preparation method thereof |
| CN205719721U (en) * | 2016-06-25 | 2016-11-23 | 南京健邦锦源医疗仪器有限公司 | A kind of Thinprep pap test film-making filter membrane cylinder |
-
2018
- 2018-07-25 CN CN201810823508.XA patent/CN109030146A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5840502A (en) * | 1994-08-31 | 1998-11-24 | Activated Cell Therapy, Inc. | Methods for enriching specific cell-types by density gradient centrifugation |
| CN1967193A (en) * | 2005-11-18 | 2007-05-23 | 上海昂佰生物医学科技有限公司 | Cell processing kit and using method therefor |
| CN101246096A (en) * | 2008-03-12 | 2008-08-20 | 郑明� | Centrifugal pellet mill and hanging cradle used for the same |
| US20140087360A1 (en) * | 2011-05-05 | 2014-03-27 | Stemcell Technologies, Inc. | Method and insert for density gradient separation |
| CN202471474U (en) * | 2011-12-02 | 2012-10-03 | 孝感奥华医疗科技有限公司 | Slide making clamp for cell slide maker |
| CN203606191U (en) * | 2013-11-26 | 2014-05-21 | 上海宇度医学科技股份有限公司 | Thin-layer liquid-based cell slide maker |
| CN205593811U (en) * | 2016-03-11 | 2016-09-21 | 厦门信道生物技术有限公司 | Concentrated smear device |
| CN106047795A (en) * | 2016-06-22 | 2016-10-26 | 杭州海世嘉生物科技有限公司 | Sample density separation liquid and preparation method thereof |
| CN205719721U (en) * | 2016-06-25 | 2016-11-23 | 南京健邦锦源医疗仪器有限公司 | A kind of Thinprep pap test film-making filter membrane cylinder |
Non-Patent Citations (1)
| Title |
|---|
| 赵风章, vol. 10, no. 2, pages 215 - 220 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111811901A (en) * | 2020-06-30 | 2020-10-23 | 郑志森 | Cell block collecting device and cell block collecting method |
| CN111811901B (en) * | 2020-06-30 | 2022-09-20 | 郑志森 | Cell block collecting device and cell block collecting method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| RU2510844C2 (en) | Container for isolation and identification of microorganism | |
| CN106238110B (en) | Use filtering and sample transfer device isolation, accumulation, characterization and/or the method for determining microorganism | |
| ITMI971490A1 (en) | READY-TO-USE CONTAINER TO OBTAIN AUTOLOGOUS FIBRIN GLUE | |
| CN102460177A (en) | System and method for automatically venting and sampling a culture specimen container | |
| CN1703621A (en) | Collection assembly | |
| EP2702144A1 (en) | Automated systems and methods for isolating regenerative cells from adipose tissue | |
| CN112899218A (en) | Dual tangential flow filtration system for exosome extraction, and preparation method and application of exosome | |
| EP3372669B1 (en) | Device for isolating cell fractions from human and animal tissues and method for the use thereof | |
| US20100034783A1 (en) | Medical kit and using method thereof | |
| CN104830896A (en) | Method for expressing proteins by using plant petal cell protoplast | |
| CN104651224A (en) | Double-tube vacuum film sperm separation method and separation device | |
| CN109030146A (en) | A kind of sectioning cells reagent, kit and application method | |
| KR20120128301A (en) | Microorganism Transport medium vessel | |
| CN108588009A (en) | A method of it detaches and activates the minimum embryonic-like stem cell of human peripheral | |
| US10160949B2 (en) | Agent for improving sperm-motility | |
| CN204569923U (en) | A kind of spermatozoa separation devices | |
| CN109486764A (en) | A kind of peripheral blood DC cell culture processes loading tumour cell excretion body | |
| JP6633512B2 (en) | Cell separation method | |
| CN113913372A (en) | Extraction protection solution for isolating and protecting mitochondria from cells | |
| RU2651171C1 (en) | Method of collecting micro-particles and micro-traces from the objects of vegetable and animal origin | |
| US10913931B1 (en) | Apparatus and method for harvesting and preparing viable stem cells | |
| CN208420487U (en) | A kind of miillpore filter pelletizer of fast demountable | |
| TWI775107B (en) | Kit and method for isolating mitochondria and use of protection solution for isolating mitochondria from cell and maintaining the activity of mitochondria | |
| RU2620304C2 (en) | Device for cell fraction isolation from human and animal tissues, and method of its application | |
| CN110241068B (en) | Method for removing phenol red in cell culture waste liquid |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20181218 |