CN109010108A - The application of the anti-skin photoage of Andrographolidi Natrii Bisulfis and suncream - Google Patents
The application of the anti-skin photoage of Andrographolidi Natrii Bisulfis and suncream Download PDFInfo
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- CN109010108A CN109010108A CN201811012551.4A CN201811012551A CN109010108A CN 109010108 A CN109010108 A CN 109010108A CN 201811012551 A CN201811012551 A CN 201811012551A CN 109010108 A CN109010108 A CN 109010108A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4973—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
- A61K31/365—Lactones
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/18—Antioxidants, e.g. antiradicals
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D307/00—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom
- C07D307/02—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings
- C07D307/34—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members
- C07D307/56—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
- C07D307/60—Two oxygen atoms, e.g. succinic anhydride
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Abstract
A kind of application of the anti-skin photoage of Andrographolidi Natrii Bisulfis and suncream, are related to skin problem prevention and control field.The application of the anti-skin photoage of the Andrographolidi Natrii Bisulfis of the embodiment of the present invention is that Andrographolidi Natrii Bisulfis is used to prepare the skin products for preventing and treating skin photoage, with for clinically skin photoage treatment or adjunctive therapy, and good drug efficacy；The suncream of the embodiment of the present invention contains the high active ingredient of this anti-skin photoage of Andrographolidi Natrii Bisulfis, expands sunscreen product type in the market, enhances user experience.
Description
Technical field
The present invention relates to skin problem prevention and control fields, and the anti-skin light of in particular to a kind of Andrographolidi Natrii Bisulfis
The application of aging and suncream.
Background technique
Skin photoage is different from normal aging, is caused by solar radiation (wherein ultraviolet radiation is principal element)
Skin multiple damages accelerate the ageing process of skin.More it is worth noting that skin photoage is also easy to develop into skin
Cancer, such as squamous cell carcinoma seriously threaten human skin health.
The clinical treatment means of light aging are based on chemical peeling, photoelectric technology, surgical operation.Wherein, traditional stripping
De- property laser therapy has such as carbon dioxide laser and bait laser and repairs wrinkle and control the effect of skin treats light aging, but strips off
Need to strip off entire epidermis in property laser therapy procedures, it is postoperative to take a long time reparation, and simultaneously with erythema, oedema,
The a series of side effects such as infection, incrustation and pigmentation.Non- exfoliative laser therapy, such as Photorejuvenation Technology can be by specific
Wide spectrum glory selection index system in papillary layer of corium and the above skin layer, but its therapeutic effect is general.Exfoliative dot matrix laser
Treatment, can targeting in low-grade fever damage zone, skin restores very fast, and side effect is less.Intense pulsed light technology is to be with flash lamp
Wide source obtains the high intensity pulses light of different spectrum segments, the treatment for photoaging skin by different optical filtering techniques.
Although there are some relatively effective skin photoage treatment means at present, some special equipments is needed just to can be carried out
Treatment, few drugs are for the clinically treatment of skin photoage or adjunctive therapy.
Summary of the invention
The purpose of the present invention is to provide a kind of application of the anti-skin photoage of Andrographolidi Natrii Bisulfis, sulfurous acid
Hydrogen andrographolide sodium is used to prepare the skin products for preventing and treating skin photoage, for clinically skin photoage
Treatment or adjunctive therapy, and good drug efficacy.
Another object of the present invention is to provide a kind of suncream, the high active ingredient containing anti-skin photoage expands
Sunscreen product type in the market enhances user experience.
The present invention solves its technical problem and adopts the following technical solutions to realize.
The present invention proposes a kind of application of anti-skin photoage of Andrographolidi Natrii Bisulfis, sodium hydrogensulfite Herba Andrographitis
Lactone is used to prepare the skin products for preventing and treating skin photoage.
Further, in a preferred embodiment of the present invention, Andrographolidi Natrii Bisulfis is by adjusting NF-kB and Nrf2
Signal path reaches anti-inflammatory oxidation resistant effect.
Further, in a preferred embodiment of the present invention, Andrographolidi Natrii Bisulfis is used to prepare treatment UVA+UVB
The skin products of radiation-induced HaCat cell light aging.
Further, in a preferred embodiment of the present invention, skin products are one of skin care item, cosmetics, drug.
Further, in a preferred embodiment of the present invention, Andrographolidi Natrii Bisulfis quality hundred in skin products
Dividing specific concentration is 0.05%~5%.
Further, in a preferred embodiment of the present invention, the preparation method of Andrographolidi Natrii Bisulfis includes following
Step:
Andrographolide is dissolved in methanol aqueous solution, andrographolide solution is obtained；Sodium hydrogensulfite is soluble in water, it obtains
Solution of sodium bisulfite；
Solution of sodium bisulfite is added in andrographolide solution, is reacted 0.5~6 hour in 40~95 DEG C；
The evaporative removal methanol under conditions of vacuum degree > 0.03MPa, 20~85 DEG C of temperature；
It is cooled to 0~10 DEG C, 1~12 hour is stood, absorbs and filter to obtain filtrate；
Acid solution is added into filtrate, is cooled to -5 DEG C~40 DEG C, crystallization is precipitated；
It will crystallize soluble in water, adjusting pH is 4.0~6.5, is cooled to -5 DEG C~30 DEG C, and sodium hydrogensulfite Herba Andrographitis is precipitated
Lactone crystallization, then filter, filter cake is dried in vacuo, white Andrographolidi Natrii Bisulfis is obtained.
Further, in a preferred embodiment of the present invention, when preparing andrographolide solution, andrographolide and methanol-water
The weight ratio of solution is 1:6~14, and the mass percent concentration of methanol aqueous solution is in 50%~100% methanol aqueous solution.
Further, in a preferred embodiment of the present invention, when preparing solution of sodium bisulfite, the weight of sodium hydrogensulfite and water
Amount is than being 1:3.5~10.
Further, in a preferred embodiment of the present invention, the additional amount of acid solution is 0.7~1.5 times of sodium hydrogensulfite
Quality.
The present invention proposes a kind of suncream, contains Andrographolidi Natrii Bisulfis, Andrographolidi Natrii Bisulfis
Mass percent concentration be 1%~2%.
The application of the anti-skin photoage of the Andrographolidi Natrii Bisulfis of the embodiment of the present invention and the beneficial effect of suncream
Fruit is: the application of the anti-skin photoage of the Andrographolidi Natrii Bisulfis of the embodiment of the present invention is in sodium hydrogensulfite Herba Andrographitis
Ester is used to prepare the skin products for preventing and treating skin photoage, is controlled with the treatment for clinically skin photoage or assist
It treats, and good drug efficacy；The suncream of the embodiment of the present invention contains the height of this anti-skin photoage of Andrographolidi Natrii Bisulfis
Active constituent expands sunscreen product type in the market, enhances user experience.
Detailed description of the invention
In order to illustrate the technical solution of the embodiments of the present invention more clearly, below will be to needed in the embodiment attached
Figure is briefly described, it should be understood that the following drawings illustrates only certain embodiments of the present invention, therefore is not construed as pair
The restriction of range for those of ordinary skill in the art without creative efforts, can also be according to this
A little attached drawings obtain other relevant attached drawings.
Fig. 1 is the cell activity result figure after the Andrographolidi Natrii Bisulfis effect of various concentration；
Fig. 2 is the Andrographolidi Natrii Bisulfis of various concentration to the cell fluorescence figure after the effect of light aged cells；
Fig. 3 is influence result figure of the Andrographolidi Natrii Bisulfis to ROS in light aged cells of various concentration；
Fig. 4 is influence result figure of the Andrographolidi Natrii Bisulfis to light aged cells anti-inflamatory levels of various concentration；
Fig. 5 is influence result figure of the Andrographolidi Natrii Bisulfis to light aged cells antioxidant levels；
Fig. 6 is the Andrographolidi Natrii Bisulfis of various concentration to light aged cells NF-kB and Nrf2 signal path
Adjusted result figure.
Specific embodiment
It in order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below will be in the embodiment of the present invention
Technical solution be clearly and completely described.The person that is not specified actual conditions in embodiment, according to normal conditions or manufacturer builds
The condition of view carries out.Reagents or instruments used without specified manufacturer is the conventional production that can be obtained by commercially available purchase
Product.
Below to the anti-skin photoage of the Andrographolidi Natrii Bisulfis of the embodiment of the present invention application and suncream into
Row illustrates.
The embodiment of the present invention provides a kind of application of anti-skin photoage of Andrographolidi Natrii Bisulfis, sodium hydrogensulfite
Andrographolide is used to prepare the skin products for preventing and treating skin photoage.
Specifically, Andrographolidi Natrii Bisulfis reaches anti-inflammatory anti-oxidant by adjusting NF-kB and Nrf2 signal path
Effect, therefore, the Andrographolidi Natrii Bisulfis HaCat cell light aging radiation-induced to UVA+UVB have protection make
With the radiation-induced HaCat cell light aging of UVA+UVB can be treated, correspondingly, Andrographolidi Natrii Bisulfis is used to prepare
The skin products for treating the radiation-induced HaCat cell light aging of UVA+UVB.
The skin products of the present embodiment refer to the product for acting on skin, mostly smearing class product, for example, skin products are
One of the skin care item of anti-skin photoage, the cosmetics of anti-skin photoage, drug of anti-skin photoage.Actually answering
In, Andrographolidi Natrii Bisulfis mass percent concentration in skin products is generally 0.05%~5%.
Andrographolidi Natrii Bisulfis in the present embodiment refers to product of the purity 99.8% or more, can directly purchase
Buy, can also be prepared according to following preparation method, the preparation method of Andrographolidi Natrii Bisulfis the following steps are included:
(1) andrographolide is dissolved in methanol aqueous solution, obtains andrographolide solution, prepare andrographolide solution
When, the weight ratio of andrographolide and methanol aqueous solution is 1:6~14, the mass percent concentration of methanol aqueous solution is 50%~
In 100% methanol aqueous solution；Sodium hydrogensulfite is soluble in water, solution of sodium bisulfite is obtained, solution of sodium bisulfite is prepared
When, the weight ratio of sodium hydrogensulfite and water is 1:3.5~10.
(2) solution of sodium bisulfite is added in andrographolide solution, is reacted 0.5~6 hour in 40~95 DEG C；
(3) the evaporative removal methanol under conditions of vacuum degree > 0.03MPa, 20~85 DEG C of temperature；
(4) it is cooled to 0~10 DEG C, 1~12 hour is stood, absorbs and filter to obtain filtrate；
(5) acid solution is added into filtrate, the additional amount of acid solution is 0.7~1.5 times of quality of sodium hydrogensulfite, cooling
To -5 DEG C~40 DEG C, crystallization is precipitated；
It (6) will crystallize soluble in water, adjusting pH is 4.0~6.5, is cooled to -5 DEG C~30 DEG C, and sodium hydrogensulfite is precipitated and wears
Heart lotus lactone crystallization, then filter, filter cake is dried in vacuo, white Andrographolidi Natrii Bisulfis is obtained.
The embodiment of the present invention also provides a kind of suncream, and containing Andrographolidi Natrii Bisulfis, sodium hydrogensulfite is worn
The mass percent concentration of heart lotus lactone is 1%~2%.
Feature and performance of the invention are described in further detail with reference to embodiments.
Embodiment 1
The present embodiment provides a kind of Andrographolidi Natrii Bisulfis, are made in accordance with the following methods:
(1) andrographolide of 350 parts by weight is dissolved in the methanol that the concentration expressed in percentage by weight of 2100 parts by weight is 100%,
Obtain andrographolide solution；The sodium hydrogensulfite of 100 parts by weight is dissolved in the water of 700 parts by weight, obtains solution of sodium bisulfite.
(2) solution of sodium bisulfite is added in andrographolide solution, is reacted 6 hours in 40 DEG C~50 DEG C.
(3) in vacuum degree > 0.03MPa, 85 DEG C of temperature are steamed methanol to the greatest extent.
(4) it is cooled to 0 DEG C, 1 hour is stood, the active carbon of 1 parts by weight is added to filter.
(5) sulfuric acid for being equivalent to sodium hydrogensulfite 0.7 times of quality times is added into filtrate, is cooled to -5 DEG C, crystallization is precipitated.
(6) crystallization of precipitation being dissolved in 40 DEG C of water of 0.6 times of crystallization, adding sodium hydroxide makes pH 4.0,
- 5 DEG C are cooled to, Andrographolidi Natrii Bisulfis crystallization is precipitated, then filter, filter cake is dried in vacuo, white sulfurous acid is obtained
Hydrogen andrographolide sodium.
The effect of Andrographolidi Natrii Bisulfis and performance are tested below by way of test.
One, the cell toxicity test of Andrographolidi Natrii Bisulfis
Cutin is immortalized with the DMEM high glucose medium culture containing 10% fetal calf serum under the conditions of 37 DEG C, 5%CO2 to be formed
Cell line HaCaT cell, when cell it is long to about 90% when, vitellophag prepares cell suspension.Adjusting cell density is
3.5x104A/ml, every 200 μ l of hole additional amount are added into 96 orifice plates, the next day change liquid, when cell it is long to about 80% when, give
Andrographolidi Natrii Bisulfis culture medium of the cell containing various concentration in different orifice plates, dosage are respectively 0 μM of (control
Group), 0.01 μM, 0.1 μM, 1 μM, 10 μM, 100 μM, 500 μM, 1000 μM, 2000 μM, cultivated for 24 hours after administration.
Using MTT colorimetric determination cell viability: taking out 96 orifice plates after cell culture, the MTT solution of 20 μ l is added, then set
4h is incubated in incubator.After incubation, the DMSO solution of 150 μ l, low-speed oscillation 10min, with enzyme mark is added in Aspirate supernatant
Instrument detects absorbance value at its 490nm.
Fig. 1 is the cell activity result figure after the Andrographolidi Natrii Bisulfis effect of various concentration.By can be in Fig. 1
Find out, the Andrographolidi Natrii Bisulfis of various concentration influences less, it follows that sodium hydrogensulfite punching cell activity
Lotus lactone is without apparent cytotoxicity.In addition, being incremented by with Andrographolidi Natrii Bisulfis concentration, cell activity constantly becomes
Change, is 10~100 μM in Andrographolidi Natrii Bisulfis concentration, cell activity is relatively stable, determines sodium hydrogensulfite punching
The best dosage of lotus lactone is 10~100 μM (10 μM, 30 μM, 100 μM.
Two, influence test of the Andrographolidi Natrii Bisulfis to light aged cells level of apoptosis
When cell it is long to about 90% when, vitellophag prepares cell suspension.Adjustment cell density is 3.5x104A/ml,
Every 2ml cell suspension is added into 35mm culture dish, the next day change liquid, when cell it is long to about 80% when, give in different culture dishes
Andrographolidi Natrii Bisulfis culture medium of the cell containing various concentration, dosage is respectively 10 μM, 30 μM, 100 μM, is given
Medicine for 24 hours after, give cell 90mJ/cm2UV radiation modeling (culture medium is sucked out before when modeling plus PBS is washed twice, and with 700
μ l PBS covers cell and carries out modeling radiation).
PBS is abandoned after modeling, culture medium culture 12h is added, abandons original fluid, and PBS is washed 2 times, and 1010 μ are added in each culture dish
33342 dye liquor of l Hoechst (10 μ l Hoechst33342+1000 μ l culture medium) is incubated for 35min in 37 DEG C of incubators, abandons
Dye liquor adds PBS to wash twice, and (the 5 μ l PI+1000 μ l Buffer A work of 1005 μ l PI dye liquors is added in each culture dish
Liquid), room temperature, which is protected from light, is incubated for 15min, abandons dye liquor, is washed twice with PBS, in fluorescence microscopy microscopic observation.
Fig. 2 is the Andrographolidi Natrii Bisulfis of various concentration to the cell fluorescence figure after the effect of light aged cells.By
The apoptosis situation that Fig. 2 can be seen that the light aged cells of unused Andrographolidi Natrii Bisulfis effect is obvious, uses bisulfite
The apoptosis situation of light aged cells after andrographolide sodium effect significantly reduces, it follows that in sodium hydrogensulfite Herba Andrographitis
Ester has hindrance function to the apoptosis of light aged cells.
Three, influence of the Andrographolidi Natrii Bisulfis to reactive oxygen species ROS in light aged cells is tested
Cell kind plate being carried out according to the step in test (two), modeling being administered, PBS is abandoned after modeling, culture medium culture is added
1h abandons original fluid, and PBS is washed 2 times, added 1000 μ l of DCFH-DA dilution (1 μ l DCFH-DA+1500 μ l culture medium), 37 DEG C of trainings
It supports to be protected from light in case and is incubated for 30min, abandon dye liquor, washed twice with PBS, fluorescence microscope.
Fig. 3 is influence result figure of the Andrographolidi Natrii Bisulfis to ROS in light aged cells of various concentration.By scheming
3 as can be seen that ROS content significantly increases (NC is nature cell, and MC is light aged cells), middle and high concentration in light aged cells
ROS induced cell apoptosis by cellular oxidation stress reaction even result in its necrosis, by Andrographolidi Natrii Bisulfis
After processing, ROS content is significantly reduced in light aged cells, wherein 100 μM of Andrographolidi Natrii Bisulfis are to light aged cells
The influence of middle ROS is most significant.
Four, influence test of the Andrographolidi Natrii Bisulfis to light aged cells anti-inflamatory levels
Cell kind plate being carried out according to the step in test (two), modeling being administered, PBS is abandoned after modeling, culture medium culture is added
12h takes culture supernatant, and -20 DEG C save backup.ELISA measure IL-1 β, IL-6, TNF-α content.
In addition, carrying out cell kind plate according to the step in test (two) is administered modeling, PBS is abandoned after modeling, and culture medium is added
2h is cultivated, culture medium is abandoned, 1000 μ l Trizol is added in each culture dish, dissolution cell is aspirated with liquid-transfering gun, leaves and takes sample-
20 DEG C save backup.QRT-PCR detect NF- κ B regulatory factor IL-1 β, IL-6, TNF-α gene expression dose.
Fig. 4 is influence result figure of the Andrographolidi Natrii Bisulfis to light aged cells anti-inflamatory levels of various concentration.
As seen from Figure 4, after Andrographolidi Natrii Bisulfis is handled, IL-1 β of light aged cells, IL-6, TNF-α contain
The gene expression dose significant decrease of amount and IL-1 β, IL-6, TNF-α, it follows that Andrographolidi Natrii Bisulfis energy
Improve the anti-inflamatory levels of light aged cells.
Five, influence test of the Andrographolidi Natrii Bisulfis to light aged cells antioxidant levels
Cell kind plate being carried out according to the step in test (two), modeling being administered, PBS is abandoned after modeling, culture medium culture is added
12h abandons culture medium, 1000 μ l Trizol is added in each culture dish, aspirates dissolution cell with liquid-transfering gun, leaves and takes -20 DEG C of sample
It saves backup.The gene expression dose of qRT-PCR detection Nrf2 downstream gene NQO1, GCLC.
Fig. 5 is influence result figure of the Andrographolidi Natrii Bisulfis to light aged cells antioxidant levels.It can be with by Fig. 5
Find out, after 100 μM of Andrographolidi Natrii Bisulfis are handled, Nrf2 downstream gene NQO1, GCLC's of light aged cells
Gene expression dose significantly increases, it follows that Andrographolidi Natrii Bisulfis can improve the anti-oxidant water of light aged cells
It is flat.
Six, Andrographolidi Natrii Bisulfis tests the adjustment effect of light aged cells NF-kB and Nrf2 signal path
Cell kind plate being carried out according to the step in test (two), modeling being administered, PBS is abandoned after modeling, it is solid that culture medium culture is added
It fixes time, abandons culture medium, washed 2 times with PBS, every hole is added 100 μ l protein lysates and leaves and takes sample with cell scraper scraping cells
This -20 DEG C save backup.Western blot detects the protein expression of Nrf2, keap1, p65, IkB α in cell.
Fig. 6 is the Andrographolidi Natrii Bisulfis of various concentration to light aged cells NF-kB and Nrf2 signal path
Adjusted result figure.As seen from Figure 6, after Andrographolidi Natrii Bisulfis is handled, Nrf2 in light aged cells,
The protein expression level of keap1 significantly increases, thus the protein expression level significant decrease or on a declining curve of p65, IkB α may be used
Know, Andrographolidi Natrii Bisulfis is adjustable light aged cells NF-kB and Nrf2 signal path.
In conclusion the application of the anti-skin photoage of the Andrographolidi Natrii Bisulfis of the embodiment of the present invention is sulfurous acid
Hydrogen andrographolide sodium is used to prepare the skin products for preventing and treating skin photoage, for clinically skin photoage
Treatment or adjunctive therapy, and good drug efficacy；The suncream of the embodiment of the present invention contains the high active ingredient of anti-skin photoage, expands
Sunscreen product type in the market enhances user experience.
Embodiments described above is a part of the embodiment of the present invention, instead of all the embodiments.Reality of the invention
The detailed description for applying example is not intended to limit the range of claimed invention, but is merely representative of selected implementation of the invention
Example.Based on the embodiments of the present invention, obtained by those of ordinary skill in the art without making creative efforts
Every other embodiment, shall fall within the protection scope of the present invention.
Claims (10)
1. a kind of application of the anti-skin photoage of Andrographolidi Natrii Bisulfis, which is characterized in that the sodium hydrogensulfite is worn
Heart lotus lactone is used to prepare the skin products for preventing and treating skin photoage.
2. the application of the anti-skin photoage of Andrographolidi Natrii Bisulfis according to claim 1, which is characterized in that institute
Andrographolidi Natrii Bisulfis is stated by adjusting NF-kB and Nrf2 signal path, reaches anti-inflammatory oxidation resistant effect.
3. the application of the anti-skin photoage of Andrographolidi Natrii Bisulfis according to claim 1, which is characterized in that institute
State the skin products that Andrographolidi Natrii Bisulfis is used to prepare the radiation-induced HaCat cell light aging for the treatment of UVA+UVB.
4. the application of the anti-skin photoage of Andrographolidi Natrii Bisulfis according to claim 1, which is characterized in that institute
Stating skin products is one of skin care item, cosmetics, drug.
5. the application of the anti-skin photoage of Andrographolidi Natrii Bisulfis according to claim 1, which is characterized in that institute
Stating Andrographolidi Natrii Bisulfis mass percent concentration in the skin products is 0.05%~5%.
6. the application of the anti-skin photoage of Andrographolidi Natrii Bisulfis according to claim 1, which is characterized in that institute
State the preparation method of Andrographolidi Natrii Bisulfis the following steps are included:
Andrographolide is dissolved in methanol aqueous solution, andrographolide solution is obtained；Sodium hydrogensulfite is soluble in water, obtain sulfurous
Sour hydrogen sodium solution；
Solution of sodium bisulfite is added in andrographolide solution, is reacted 0.5~6 hour in 40~95 DEG C；
The evaporative removal methanol under conditions of vacuum degree > 0.03MPa, 20~85 DEG C of temperature；
It is cooled to 0~10 DEG C, 1~12 hour is stood, absorbs and filter to obtain filtrate；
Acid solution is added into filtrate, is cooled to -5 DEG C~40 DEG C, crystallization is precipitated；
It will crystallize soluble in water, adjusting pH is 4.0~6.5, is cooled to -5 DEG C~30 DEG C, and Andrographolidi Natrii Bisulfis is precipitated
Crystallization, then filter, filter cake is dried in vacuo, white Andrographolidi Natrii Bisulfis is obtained.
7. the application of the anti-skin photoage of Andrographolidi Natrii Bisulfis according to claim 6, which is characterized in that match
When andrographolide solution processed, the weight ratio of the andrographolide and the methanol aqueous solution is 1:6~14, the methanol-water
In the methanol aqueous solution that the mass percent concentration of solution is 50%~100%.
8. the application of the anti-skin photoage of Andrographolidi Natrii Bisulfis according to claim 6, which is characterized in that match
When solution of sodium bisulfite processed, the weight ratio of the sodium hydrogensulfite and water is 1:3.5~10.
9. the application of the anti-skin photoage of Andrographolidi Natrii Bisulfis according to claim 6, which is characterized in that institute
The additional amount for stating acid solution is 0.7~1.5 times of quality of sodium hydrogensulfite.
10. a kind of suncream, which is characterized in that it contains Andrographolidi Natrii Bisulfis, the sodium hydrogensulfite Herba Andrographitis
The mass percent concentration of lactone is 1%~2%.
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| CN201811012551.4A CN109010108A (en) | 2018-08-31 | 2018-08-31 | The application of the anti-skin photoage of Andrographolidi Natrii Bisulfis and suncream |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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