CN108998838A - A kind of method that laccase treatment prepares porous sugarcane fiber material - Google Patents
A kind of method that laccase treatment prepares porous sugarcane fiber material Download PDFInfo
- Publication number
- CN108998838A CN108998838A CN201810679493.4A CN201810679493A CN108998838A CN 108998838 A CN108998838 A CN 108998838A CN 201810679493 A CN201810679493 A CN 201810679493A CN 108998838 A CN108998838 A CN 108998838A
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- CN
- China
- Prior art keywords
- bagasse
- laccase
- porous
- fiber material
- sugarcane fiber
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000002657 fibrous material Substances 0.000 title claims abstract description 25
- 108010029541 Laccase Proteins 0.000 title claims abstract description 20
- 238000000034 method Methods 0.000 title claims abstract description 18
- 240000000111 Saccharum officinarum Species 0.000 title claims abstract description 17
- 235000007201 Saccharum officinarum Nutrition 0.000 title claims abstract description 17
- 241000609240 Ambelania acida Species 0.000 claims abstract description 38
- 239000010905 bagasse Substances 0.000 claims abstract description 38
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 108090000790 Enzymes Proteins 0.000 claims description 13
- 102000004190 Enzymes Human genes 0.000 claims description 13
- 238000006243 chemical reaction Methods 0.000 claims description 9
- 238000005406 washing Methods 0.000 claims description 6
- 239000002253 acid Substances 0.000 claims description 3
- 238000004064 recycling Methods 0.000 claims description 3
- 239000011148 porous material Substances 0.000 abstract description 10
- 230000001681 protective effect Effects 0.000 abstract description 2
- 239000000835 fiber Substances 0.000 description 9
- 239000000463 material Substances 0.000 description 8
- 238000010521 absorption reaction Methods 0.000 description 6
- 239000003513 alkali Substances 0.000 description 6
- 239000002994 raw material Substances 0.000 description 6
- 230000007935 neutral effect Effects 0.000 description 4
- 239000002028 Biomass Substances 0.000 description 2
- 229920002488 Hemicellulose Polymers 0.000 description 2
- 238000007664 blowing Methods 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000004566 building material Substances 0.000 description 1
- 239000003575 carbonaceous material Substances 0.000 description 1
- 238000003763 carbonization Methods 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 210000003850 cellular structure Anatomy 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000004880 explosion Methods 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000007769 metal material Substances 0.000 description 1
- 235000021110 pickles Nutrition 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
Classifications
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01C—CHEMICAL OR BIOLOGICAL TREATMENT OF NATURAL FILAMENTARY OR FIBROUS MATERIAL TO OBTAIN FILAMENTS OR FIBRES FOR SPINNING; CARBONISING RAGS TO RECOVER ANIMAL FIBRES
- D01C1/00—Treatment of vegetable material
Landscapes
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Textile Engineering (AREA)
- Chemical Or Physical Treatment Of Fibers (AREA)
- Enzymes And Modification Thereof (AREA)
- Paper (AREA)
Abstract
The invention discloses a kind of methods that laccase treatment prepares porous sugarcane fiber material, are handled using laccase bagasse, prepare porous sugarcane fiber material.The present invention control effectively to the average pore size of porous fibrous material obtained, pore-size distribution, specific surface area by control treatment conditions, easy to operate, environmentally protective.
Description
Technical field
The present invention relates to a kind of method that laccase treatment prepares porous sugarcane fiber material, belong to chemical industry, building materials, environmental protection,
With the fields such as pulp and paper technology.
Background technique
China's non-timber resource is abundant, and wherein bagasse is a kind of renewable fibre resources low in cost and environmental-friendly.
For Yunnan Province's bagasse resource, Sugarcane accounts for the 23% of this province non-timber resource, saves greatly as sugar is produced, however after squeezing sugar
Bagasse utilization rate is not high, and most of all to burn or discard, only sub-fraction is used for papermaking, and there are also sub-fractions to make biological wine
Refining system and wastewater treatment etc..Environment is not only polluted, the waste of resource is more caused.Therefore, the high efficient resourcing benefit of bagasse is explored
With being of great significance.
The research range of porous material is very wide, study at present it is more for porous semi-conductor material, porous metal material etc.,
However the research for preparing porous fibrous material for bagasse is less.The adjustability in porous fibrous material aperture has widened porous fibre
The application range of material, such as pressed density plate, porous fibre carbonization prepare high-specific surface area carbon material, as adsorbent material.
The aperture of effective regulation fiber is the effective means for improving fiber added value and its economic benefit.Currently used for regulating and controlling fiber holes
There are mainly two types of the methods of gap: one is that can drop low-fiber porosity by squeezing or dry method, such as passing through wet pressing
It squeezes, the foreign technology discovery of control sugarcane fiber nanoporosity when pressure minimum (21MPa), can reduce most slurries
The nanoporosity of material is conducive to the conversion of biomass；Another kind is effectively to increase fiber by handling raw material
Porosity increase wheat straw surface porosity factor if Zhou Min is by steam explosion, improve the dissolution of hemicellulose.
Summary of the invention
The object of the present invention is to provide a kind of methods that laccase treatment prepares porous sugarcane fiber material, including following step
It is rapid:
It (1) is in mass ratio that 1:4-1:10 is mixed and is placed in reactor with water by bagasse, the diluted acid for being 0.01-1mol/L with concentration
Or it is 3.2-9 that diluted alkaline, which adjusts the pH value of the suspension of bagasse and water, adjusting temperature is 15-60 DEG C, after being passed through air 0-10min,
Laccase 40-150U is added by every gram of over dry bagasse (bagasse for being entirely free of moisture is over dry bagasse) relatively, is uniformly mixed, instead
60-400min is answered, air can be passed through or be not passed through in reaction process；
(2) it is filtered after reaction with 200-400 mesh filter screen, to neutrality to get porous sugarcane fiber material, enzyme solution can be returned for washing
Receive recycling.
Principle: bagasse is a kind of renewable biomass resources of meso-hole structure, have short texture, degree of lignification compared with
Low, hole holds the features such as larger, orderly and adjustable cellular structure, therefore bagasse passes through certain processing, it can be improved extensively
Potential applied to every field.The selectivity of laccase biological treatment delignification is preferable, can make lignin degradation during processing
Dissolution, by the control to treatment process conditional, so as to realize the control to Bagasse Material aperture.Using laccase to original
Material is handled, and control raw material aperture changes in a certain range, and is a kind of method of mild control raw material varying aperture.This
Invention is handled bagasse using laccase, prepares porous sugarcane fiber material, by controlling treatment conditions, to obtained porous
Average pore size, pore-size distribution, the specific surface area of fibrous material control effectively, so that the variation of material structure property is controlled,
It is of great significance to the high-efficiency resource recycling for improving bagasse.
The beneficial effects of the present invention are:
(1) present invention can effectively regulate and control porous fibrous material obtained by control laccase to the treatment conditions of bagasse
Average pore size, pore-size distribution and surface area improve the application potential of porous sugarcane fiber material, have widened the application of bagasse
Face；
(2) porous fibrous material average pore size made from is 5.0-8.5nm, specific surface area 1.0-1.2m2The hole of/g, 2-10nm
It is 5.48nm, specific surface area 0.8995m that gap representative fraction, which is greater than the untreated bagasse average pore size of 60%(,2The hole of/g, 2-10nm
46.20%) gap ratio is；Under room temperature, more untreated bagasse Alkali absorption amount in 20min, which improves, is up to the untreated bagasse of 85%(
Alkali absorption amount is up to 8.03g/100g over dry bagasse in 20min)；
(3) enzyme has specificity, is handled with laccase raw material, will not act on the cellulose and hemicellulose of raw material, because
This ensure that the quality of material after processing；
(4) operation of the present invention is simple, and raw material is renewable resource bagasse, and cheap, environmentally protective, environmental-friendly.
Specific embodiment
Below with reference to embodiment, invention is further described in detail, but the scope of the present invention is not limited in described
Hold.
Embodiment 1
(1) in mass ratio it is that 1:6 is mixed and is placed in reactor with water by bagasse, adjusts pH with the pickle that concentration is 0.1mol/L
For value to 5.5, temperature is 50 DEG C in reactor, is uniformly passed through air 5min, and enzyme solution is added, and (every gram of over dry bagasse is added relatively
95U laccase) and be uniformly mixed, blowing air reacts 300min；
(2) it is filtered after reaction with 200 mesh filter screens, with originally water washing to neutral up to porous sugarcane fiber material, simultaneously
Recycle enzyme solution.
Porous fibrous material average pore size manufactured in the present embodiment is 8.0-8.5nm, and the hole ratio of 2-10nm is 65%,
Specific surface area is 1.17-1.20m2/g；Under room temperature, Alkali absorption amount of the more untreated bagasse in 20min improves 85%.
Embodiment 2
(1) in mass ratio it is that 1:8 is mixed and is placed in reactor with water by bagasse, is adjusted with the sig water that concentration is 0.01mol/L
PH value is uniformly mixed, temperature is 60 DEG C in reactor, is uniformly passed through air 10min, and enzyme solution (every gram of over dry relatively is added to 7
95U laccase is added in bagasse) and be uniformly mixed, blowing air reacts 200min；
(2) it is filtered after reaction with 300 mesh filter screens, with originally water washing to neutral up to porous sugarcane fiber material, simultaneously
Recycle enzyme solution.
Porous fibrous material average pore size manufactured in the present embodiment is 7.0-7.5nm, and the hole ratio of 2-10nm is 62%,
Specific surface area is 1.15-1.18m2/g；Under room temperature, Alkali absorption amount of the more untreated bagasse in 20min improves 72%.
Embodiment 3
It (1) is in mass ratio that 1:4 is mixed and is placed in reactor with water by bagasse, the dilute acid for adjusting pH value for being 0.5mol/L with concentration
To 3.2, it is uniformly mixed, temperature is 15 DEG C in reactor, and enzyme solution (40U laccase is added in every gram of over dry bagasse relatively) is added and mixes
Uniformly, 200min is reacted；
(2) it is filtered after reaction with 400 mesh filter screens, with originally water washing to neutral up to porous sugarcane fiber material, simultaneously
Recycle enzyme solution.
Porous fibrous material average pore size manufactured in the present embodiment is 5.0-5.5nm, and the hole ratio of 2-10nm is 60%,
Specific surface area is 1.00-1.05m2/g；Under room temperature, Alkali absorption amount of the more untreated bagasse in 20min improves 53%.
Embodiment 4
(1) enzyme solution recycled in bagasse and embodiment 3 (clear water is added when enzyme liquid amount is inadequate to supply) is mixed for 1:10 in mass ratio
Conjunction is placed in reactor, is adjusted pH value to 9 with the sig water that concentration is 1.0mol/L, is uniformly mixed, temperature is 60 in reactor
DEG C, enzyme solution (150U laccase is added in every gram of over dry bagasse relatively) is added and is uniformly mixed, reacts 60min；
(2) it is filtered after reaction with 200 mesh filter screens, with originally water washing to neutral up to porous sugarcane fiber material, simultaneously
Recycle enzyme solution.
Porous fibrous material average pore size manufactured in the present embodiment is 6.0-6.5nm, and the hole ratio of 2-10nm is 61%,
Specific surface area 1.17-1.19m2/g；Under room temperature, Alkali absorption amount of the more untreated bagasse in 20min improves 80%.
Claims (7)
1. a kind of method that laccase treatment prepares porous sugarcane fiber material, comprising the following steps:
(1) bagasse being mixed with water and is placed in reactor, adjusting pH value is 3.2-9, and adjusting temperature is 15-60 DEG C, laccase is added,
It is uniformly mixed, reacts 60-400min；
(2) it is filtered after reaction with 200-400 mesh filter screen, washing is to neutrality to get porous sugarcane fiber material.
2. the method according to claim 1, wherein the mass ratio of bagasse and water is 1:4-1:10.
3. the method according to claim 1, wherein the diluted acid or diluted alkaline that are 0.01-1mol/L with concentration are adjusted
PH value.
4. the method according to claim 1, wherein being first passed through air 0-10min before laccase is added.
5. the method according to claim 1, wherein laccase 40-150U is added in every gram of over dry bagasse relatively.
6. the method according to claim 1, wherein air is passed through or is not passed through in reaction process.
7. the method according to claim 1, wherein the method also includes recycling and reusing enzyme solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810679493.4A CN108998838A (en) | 2018-06-27 | 2018-06-27 | A kind of method that laccase treatment prepares porous sugarcane fiber material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810679493.4A CN108998838A (en) | 2018-06-27 | 2018-06-27 | A kind of method that laccase treatment prepares porous sugarcane fiber material |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108998838A true CN108998838A (en) | 2018-12-14 |
Family
ID=64600867
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810679493.4A Pending CN108998838A (en) | 2018-06-27 | 2018-06-27 | A kind of method that laccase treatment prepares porous sugarcane fiber material |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108998838A (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103556517A (en) * | 2013-10-31 | 2014-02-05 | 昆明理工大学 | Method for improving oxygen-alkali pulping effect through laccase pretreatment |
-
2018
- 2018-06-27 CN CN201810679493.4A patent/CN108998838A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103556517A (en) * | 2013-10-31 | 2014-02-05 | 昆明理工大学 | Method for improving oxygen-alkali pulping effect through laccase pretreatment |
Non-Patent Citations (1)
| Title |
|---|
| 李娇阳等: "漆酶预处理前后蔗渣半纤维素的提取及性质研究", 《林产化学与工业》 * |
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| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20181214 |