CN108981963B - Temperature measuring device based on wireless power supply - Google Patents
Temperature measuring device based on wireless power supply Download PDFInfo
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- CN108981963B CN108981963B CN201811141561.8A CN201811141561A CN108981963B CN 108981963 B CN108981963 B CN 108981963B CN 201811141561 A CN201811141561 A CN 201811141561A CN 108981963 B CN108981963 B CN 108981963B
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- 238000012545 processing Methods 0.000 claims abstract description 48
- 239000000523 sample Substances 0.000 claims description 45
- 239000003153 chemical reaction reagent Substances 0.000 claims description 15
- 238000005516 engineering process Methods 0.000 claims description 11
- 230000005540 biological transmission Effects 0.000 claims description 10
- 230000003287 optical effect Effects 0.000 claims description 7
- 238000004891 communication Methods 0.000 claims description 6
- 230000000694 effects Effects 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 4
- 229910052751 metal Inorganic materials 0.000 claims description 3
- 239000002184 metal Substances 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 2
- 229910000859 α-Fe Inorganic materials 0.000 claims description 2
- 238000009529 body temperature measurement Methods 0.000 description 13
- 238000006243 chemical reaction Methods 0.000 description 9
- 230000006698 induction Effects 0.000 description 4
- 230000033001 locomotion Effects 0.000 description 4
- 238000003752 polymerase chain reaction Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- 230000004544 DNA amplification Effects 0.000 description 1
- 229910000861 Mg alloy Inorganic materials 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- SNAAJJQQZSMGQD-UHFFFAOYSA-N aluminum magnesium Chemical compound [Mg].[Al] SNAAJJQQZSMGQD-UHFFFAOYSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000000149 argon plasma sintering Methods 0.000 description 1
- 230000001351 cycling effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000008054 signal transmission Effects 0.000 description 1
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01K—MEASURING TEMPERATURE; MEASURING QUANTITY OF HEAT; THERMALLY-SENSITIVE ELEMENTS NOT OTHERWISE PROVIDED FOR
- G01K13/00—Thermometers specially adapted for specific purposes
-
- H—ELECTRICITY
- H02—GENERATION; CONVERSION OR DISTRIBUTION OF ELECTRIC POWER
- H02J—CIRCUIT ARRANGEMENTS OR SYSTEMS FOR SUPPLYING OR DISTRIBUTING ELECTRIC POWER; SYSTEMS FOR STORING ELECTRIC ENERGY
- H02J50/00—Circuit arrangements or systems for wireless supply or distribution of electric power
-
- H—ELECTRICITY
- H02—GENERATION; CONVERSION OR DISTRIBUTION OF ELECTRIC POWER
- H02J—CIRCUIT ARRANGEMENTS OR SYSTEMS FOR SUPPLYING OR DISTRIBUTING ELECTRIC POWER; SYSTEMS FOR STORING ELECTRIC ENERGY
- H02J50/00—Circuit arrangements or systems for wireless supply or distribution of electric power
- H02J50/70—Circuit arrangements or systems for wireless supply or distribution of electric power involving the reduction of electric, magnetic or electromagnetic leakage fields
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- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Computer Networks & Wireless Communication (AREA)
- Power Engineering (AREA)
- General Physics & Mathematics (AREA)
- Electromagnetism (AREA)
- Measuring Temperature Or Quantity Of Heat (AREA)
Abstract
The invention relates to a temperature measuring device based on wireless power supply, which consists of a temperature sensor 1, a rotating body sample disc 2, a temperature signal processing unit 3 and a stepping motor 4, wherein the temperature sensor 1 is arranged in the rotating body sample disc 2 and is connected with the temperature signal processing unit 3, the temperature signal processing unit 3 and the rotating body sample disc 2 are arranged on a rotating shaft of the stepping motor 4, and synchronously rotate with the temperature sensor 1 and the rotating body sample disc 2 under the drive of the motor, so that temperature signals are acquired and emitted in the rotation of the sample.
Description
Technical field:
the invention relates to a temperature measuring device, in particular to a temperature measuring device suitable for polymerase chain reaction variable-temperature PCR reaction.
The background technology is as follows:
the polymerase chain reaction (Polymerase Chain Reaction, PCR) is a molecular biological reaction for DNA amplification. One of the key factors for success or failure of the variable temperature PCR reaction is the control of the temperature in the temperature cycling condition required by the reaction system. Temperature monitoring is an important element of the PCR reaction. The thermal cycle system of the traditional PCR is divided into two types of contact temperature measurement and non-contact temperature measurement: the contact type temperature measurement can directly place the temperature sensor near the sample tube, so that the temperature of the sample can be accurately reflected; the non-contact temperature measurement is usually infrared temperature measurement, so that the problems of poor precision and great influence by environment exist.
The contact type temperature sensor probes are used for measuring the temperature, the precision is high, the stability is good, but the measured object is usually a fixed or guide rail type moving sample disc. The temperature of the sample tray of the rotating body is monitored, and the traditional contact type temperature measurement has high requirements on the arrangement circuit because the sample is in a moving state, so that the temperature of the sample can not be estimated only by indirectly measuring the temperature of media such as water or air, or the temperature of the sample can be estimated by adopting non-contact type temperature measurement, but the traditional contact type temperature measurement has great defects. The traditional contact type temperature measurement mode can not measure on a rotating object, and can not accurately and quickly control the temperature.
The invention provides a temperature measuring device for solving the problem of high-precision temperature acquisition of a rotating body sample disk and ensuring the accuracy and stability of the rotating body sample disk.
The invention comprises the following steps:
the invention provides the rotating body sample disk temperature measuring device with high precision and high stability, which not only realizes high reliability and high stability of traditional reagent disk contact temperature measurement, but also realizes accurate wireless data transmission.
The device of the invention is a part of the invention of the PCR instrument, and only the main composition and structural view of the invention are reserved, and the upper computer software, the shell, the upper cover and part of the motion control structure of the PCR instrument are not described herein.
The temperature measuring device of the invention is shown in fig. 1, and comprises a temperature sensor 1, a rotating body sample disc 2, a temperature signal processing unit 3 and a stepping motor 4, wherein the temperature sensor 1 is arranged in the rotating body sample disc 2 and connected with the temperature signal processing unit 3, and the temperature signal processing unit 3 and the rotating body sample disc 2 are arranged on the rotating shaft of the stepping motor 4.
The temperature sensor 1 adopts a high-precision miniature NTC heat-sensitive sensor, a sensor probe is arranged in the rotary body sample tray 2, the installation position of the sensor probe is positioned between two reagent tubes and is close to the position of 1/3 height of the bottom of the reagent tube, and the accurate measurement of the sample temperature is realized.
The rotating body sample tray 2 is a sample placement device, more than 1 reagent tube can be placed, the reagent tube can rotate along with the rotating body sample tray 2, the rotating reagent tray is made of aluminum magnesium alloy and has good heat conductivity, the sensor is embedded into the rotating reagent tray, the tail end connecting line of the sensor is connected with the temperature signal acquisition module 302 in the temperature signal processing unit 3, the temperature sensor 1 transmits a temperature signal to the temperature signal processing unit 3 in the rotating process, and the temperature data is reacted to a reading window through upper computer software, so that the temperature data is obtained.
The temperature signal processing unit 3, as shown in fig. 2, comprises a light shield 301, a temperature signal acquisition module 302, a shielding cover 303, a receiving coil 304, a transmitting coil 305, a shielding cover 306, and a temperature signal processing module 307.
The shielding cover 303 of the temperature signal processing unit 3 is adhered to the temperature signal acquisition module 302, the receiving coil 304 is welded on the temperature signal acquisition module 302, and the shielding cover 303 is adhered to the receiving coil. The light shield 301, the temperature signal acquisition module 302, the shielding cover 303 and the receiving coil 304 are taken as a component, the component and the rotating body sample disk 2 are arranged on the rotating shaft of the stepping motor 4, and the component and the temperature sensor 1 and the rotating body sample disk 2 synchronously rotate under the driving of the motor, so that the temperature signal acquisition and the emission in the rotating movement of the sample are realized.
Wherein the shield 306 is bonded to the temperature signal processing module 307, and the receiving coil 305 is welded to the temperature signal processing module 307 and bonded within the shield 306. The transmitting coil 305, the shielding case 306 and the temperature signal processing module 307 are used as a component which is fixedly arranged on the structural support, the component is fixed, the receiving and processing of the temperature signal are realized, the temperature signal is transmitted to the microcontroller for data processing and uploading, and the temperature is displayed in the upper computer software.
Wherein the temperature signal acquisition module 302, the shield 303, the receiving coil 304 and the transmitting coil 305, the shield 306, and the temperature signal processing module 307 are concentrically mounted in parallel; the shield 303 and shield 306 are nested to ensure that the reagent disk rotates a secure distance.
The device combines wireless power supply and visible light communication VLC (Visible Light Communication) technology. The wireless power supply technology is convenient and quick, and no physical connection is needed; the visible light communication technology has high transmitting power, does not need to apply for radio frequency spectrum evidence, and has no electromagnetic interference. The two technologies are combined together, and the temperature signal processing unit 3 is designed to realize the temperature monitoring of the samples in the rotating body sample tray, so that the temperature sensor is a brand new data processing and temperature measuring mode.
In the temperature signal processing unit 3 of the device, the temperature signal acquisition module 302 moving along with the rotating body sample disk 2 adopts a wireless power supply technology, adopts a high-integration wireless power supply chip, a receiving coil 304 and a transmitting coil 305, is arranged in a shielding cover 303 and a shielding cover 306 which are made of domestic advanced special ferrite materials, solves the voltage supply of the temperature acquisition end of the rotating body sample disk 2, avoids the eddy current effect generated by the transmitting coil 305 and the receiving coil 306 on nearby metal parts, avoids the increase of unit power consumption, and improves the wireless power supply efficiency and stability.
In the temperature signal processing unit 3 in the device, the temperature data transmission adopts the visible light communication technology, and the LED is utilized to realize the emerging data transmission mode of transmitting the temperature signal without physical connection through the conversion of the photoelectric signal, wherein the LED has the advantages of high brightness, low power consumption, long service life, small size, environmental protection, high response sensitivity, good modulation characteristic and the like.
The invention mainly applies a visible light short-distance transmission technology, the transmission principle and model thereof are that LED radiation light is processed as spherical waves, and spherical light wave propagation expression is deduced by Maxwell equation sets:wherein E is 0 The electric field intensity of the wave source; w is the angular frequency of the light wave, and t is time; k is the wave vector of the light wave in the propagation direction, which is mainly dependent on the wavelength λ of the light; r is a space coordinate; psi 0 Is the initial phase. Therefore, the divergence angle of the visible LED is larger, and the light signal is convenient to receive, so that the white light LED is adopted in the invention. The analog signals collected by the temperature sensor are filtered and amplified, then the LED is driven to emit light, the light signals are transmitted in the atmosphere, the light signals are received by the photoelectric detector, the light signals are converted into electric signals, and the electric signals are amplified and shaped to restore the temperature value.
The temperature signal processing unit 3 of the device is a specific application of the principle in the invention, and the analog signals acquired by the temperature sensor are subjected to data processing by the high-integration AD chip and the microprocessor to drive the LED light source so as to realize the emission of the temperature signals; the optical signal is received by the photodetector, converted into an electrical signal, and the temperature signal is reduced to obtain a temperature measurement value after the conversion by the temperature signal processing module 307.
Description of the drawings:
FIG. 1 is a diagram showing the main modules of a temperature measuring device
1-temperature sensor
2-rotator sample tray
3-temperature signal processing unit
4-step motor
FIG. 2 is a detailed structural assembly relationship diagram of the temperature signal processing unit 3 in FIG. 1
301-sunshade
302-temperature signal acquisition module
303-shielding case
304-receiving coil
305-transmitting coil
306-shielding case
307-temperature signal processing module
The specific embodiment is as follows:
the device aims to solve the problem of high-precision temperature acquisition of the rotating body sample plate, and the high-precision of the temperature acquisition of the rotating body sample plate needs to be ensured, and the accuracy and the stability of signal transmission need to be ensured.
The implementation of the temperature measuring device of the present invention will be described in detail with reference to fig. 1 and 2.
Fig. 1 is a main component of the device of the invention, which consists of a temperature sensor 1, a rotating body sample disk 2 and a temperature signal processing unit 3.
Fig. 2 is a detailed composition and structural illustration of a portion of the temperature signal processing unit 3 in fig. 1, which is composed of a light shield 301, a temperature signal acquisition module 302, a shield 303, a receiving coil 304, a transmitting coil 305, a shield 306, and a temperature signal processing module 307.
The wireless power supply function in the device of the invention is realized: with advanced power supply chips, two air core induction coils (receiving coil 304 and transmitting coil 305) can be spatially isolated, and the conversion efficiency and stability in motion can fully meet the requirements. Because the induction voltages of the receiving coil 304 and the transmitting coil 305 can generate eddy current effect on nearby metal modules, generate a large amount of heat and consume power, electromagnetic shielding treatment can be performed on the two coils, a shielding case 303 and a shielding case 306 are designed, the two shielding cases are arranged in a nested manner inside and outside, and the induction coils are arranged in the shielding cases, so that induction power supply can be realized, the eddy current effect is reduced, and the power consumption is reduced.
The temperature signal acquisition is implemented by adopting a high-precision miniature NTC thermosensitive temperature sensor (+ -0.1 ℃) through a temperature sensor 1 and a temperature signal acquisition module 302, arranging a temperature probe at the position of a rotating body sample disc close to a reagent tube, transmitting an acquired temperature analog signal to a high-integration AD chip of the temperature signal acquisition module 302, carrying out AD conversion, transmitting the acquired temperature analog signal to a microprocessor for data processing, and driving an LED light source by the temperature signal processed by the microprocessor.
The LED light source is used as a photoelectric transmission light source, and the radiation light is approximately spherical wave for processing, so that the divergence angle is larger, and the light signal can be received conveniently. Meanwhile, the wavelength of visible light is similar to or even smaller than the size of dust and gas molecules in indoor atmosphere, light scattering and absorption are easy to generate, so that serious attenuation of signals is caused, and in addition, the background light of an artificial light source can also influence the performance of a system.
The implementation process of the visible light communication technology in the invention is to analyze the temperature value digitally by adopting a processing chip MCU, the temperature resolution is 0.01 ℃, for example, 26.00 ℃ is converted into an integer bit 2600, the binary representation is 101000101000, and in the transmission of photoelectric signals, in order to ensure the characteristics of the temperature signals, the beginning of the 5ms optical representation signals can be sent out under the condition of continuously no light. The MCU processes 10000101000101000 data, each bit lasting 5ms, requiring a total of 17 bits to be transmitted for 85ms, where 0000101000101000 binary numbers represent 2600. The first bit in the photo signal is the high start bit, and is irrelevant to data, and the remaining 16 bits represent an integer temperature value, which can represent 0.00 ℃ to 655.35 ℃.1 represents the light emission of the LED, 0 represents the non-light emission of the LED, the rest of the time is the non-light emission state, the temperature signal is emitted every 500ms, that is, 85ms is the start bit and data transmission stage, and 415ms is the waiting stage (no light signal in this stage). Thereby converting the digital signal into an optical signal.
In the device, the photoelectric detector receives the optical signal emitted by the temperature signal acquisition module 302 at high speed, and converts the optical signal into a digital signal. Analysis of digital signals: the MCU reads the level of this bit once every 1 ms. If there is a low level of more than 100ms, this stage is a waiting stage, when the condition is satisfied and the read level is high, the start bit level is received, and since the start level is 5ms, the read level should still be high after 2ms, then the read and recording is performed once every 5ms, and the total 16 bits of data is obtained, namely 100 times of the temperature value. The 86ms of the start bit received should be low, representing the completion of the transmission, and enter the wait phase of the signal.
The experimental data of the advantages of the temperature measuring device of the present invention compared with the existing device are as follows:
| object for measuring temperature | Temperature measurement accuracy | Resolution of temperature measurement | Energy consumption | |
| The device of the invention | Rotating body | ±0.1℃ | 0.01℃ | ＞0.5w |
| Existing device 1 | With or without movement by guide rails | ±0.3℃ | 0.1℃ | ＜0.5w |
Claims (4)
1. The temperature measuring device based on wireless power supply is characterized by comprising a temperature sensor (1), a rotating body sample disc (2), a temperature signal processing unit (3) and a stepping motor (4), wherein the temperature sensor (1) is arranged in the rotating body sample disc (2) and connected with the temperature signal processing unit (3), and the temperature signal processing unit (3) and the rotating body sample disc (2) are arranged on a rotating shaft of the stepping motor (4);
the temperature sensor (1) is arranged in the middle of the rotating body sample disc (2), the tail end of the temperature sensor is connected with a temperature signal acquisition module (302) in the temperature signal processing unit (3), and the temperature sensor (1) transmits a temperature signal to the temperature signal processing unit (3) in the rotating process;
the temperature signal processing unit (3) consists of a light shield (301), a temperature signal acquisition module (302), a first shielding cover (303), a receiving coil (304), a transmitting coil (305), a second shielding cover (306) and a temperature signal processing module (307), wherein the first shielding cover (303) is adhered to the temperature signal acquisition module (302), the receiving coil (304) is welded to the temperature signal acquisition module (302), the light shield (301), the temperature signal acquisition module (302), the first shielding cover (303) and the receiving coil (304) are adhered to the inside of the first shielding cover (303) as a component, and the first shielding cover and the second shielding cover and the rotating body sample disc (2) are arranged on a rotating shaft of the stepping motor (4);
a second shielding cover (306) in the temperature signal processing unit (3) is adhered to the temperature signal processing module (307), and the transmitting coil (305) is welded on the temperature signal processing module (307) and adhered to the second shielding cover (306); the transmitting coil (305), the second shielding case (306) and the temperature signal processing module (307) are taken as a component, and are fixedly arranged on the structural support and are fixed and do not move;
a temperature signal acquisition module (302), a first shielding cover (303), a receiving coil (304) and a transmitting coil (305), a second shielding cover (306) and a temperature signal processing module (307) in the temperature signal processing unit (3) are concentrically and parallelly arranged; the first shielding case (303) and the second shielding case (306) are nested and installed at a safe distance to ensure that the reagent disk rotates;
in the temperature signal processing unit (3), the temperature signal acquisition module (302) moving along with the rotating body sample disk (2) adopts a wireless power supply technology, adopts a high-integration wireless power supply chip, a receiving coil (304) and a transmitting coil (305), is arranged in a first shielding case (303) and a second shielding case (306) which are made of ferrite materials, solves the voltage supply of the temperature acquisition end of the rotating body sample disk (2), and avoids the eddy current effect generated by the transmitting coil (305) and the receiving coil (304) on nearby metal pieces;
in the temperature signal processing unit (3), a visible light communication technology is adopted for temperature data transmission, a white light LED is utilized to filter and amplify an analog signal acquired by a temperature sensor, the LED is driven to emit light, the temperature signal is transmitted in the atmosphere, an optical signal is received through a photoelectric detector, the optical signal is converted into an electric signal, and the electric signal is amplified and shaped to restore a temperature value.
2. The temperature measuring device according to claim 1, characterized in that the temperature sensor (1) is a miniature NTC thermal sensor, the sensor probe is placed in the rotating body sample disk (2), the mounting position of which is located in the middle of the two reagent tubes.
3. The temperature measuring device according to claim 1, characterized in that the temperature sensor (1) is mounted in the middle of the two reagent vessels at a distance of 1/3 of the height from the bottom of the reagent vessels.
4. The temperature measuring device according to claim 1, wherein the rotating body sample tray (2) is a sample placement device in which more than 1 reagent tube can be placed, and the reagent tube can be rotated along with the rotating body sample tray (2).
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811141561.8A CN108981963B (en) | 2018-09-28 | 2018-09-28 | Temperature measuring device based on wireless power supply |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811141561.8A CN108981963B (en) | 2018-09-28 | 2018-09-28 | Temperature measuring device based on wireless power supply |
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| CN108981963B true CN108981963B (en) | 2023-12-19 |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108998371A (en) * | 2018-09-28 | 2018-12-14 | 北京金豪制药股份有限公司 | A kind of PCR temperature regulating device of low lift pump induction heating |
| CN115165179B (en) * | 2022-06-29 | 2023-12-12 | 广东高标智能科技股份有限公司 | Torque detection device |
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|---|---|---|---|---|
| CN202947801U (en) * | 2012-11-28 | 2013-05-22 | 河南科技大学东海硅产业节能技术研究院 | Wireless temperature measuring device of rotary mechanical body |
| WO2016136919A1 (en) * | 2015-02-25 | 2016-09-01 | 株式会社山本金属製作所 | Temperature measurement device |
| CN108254102A (en) * | 2017-11-30 | 2018-07-06 | 北京原力辰超导技术有限公司 | A kind of high temperature superconductor coil fever detection device |
| CN108279124A (en) * | 2018-01-24 | 2018-07-13 | 常州克劳诺斯特种轴承制造有限公司 | Moving-coil detects bearing |
| CN209310946U (en) * | 2018-09-28 | 2019-08-27 | 北京金豪制药股份有限公司 | A kind of temperature measuring equipment based on wireless power |
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2018
- 2018-09-28 CN CN201811141561.8A patent/CN108981963B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202947801U (en) * | 2012-11-28 | 2013-05-22 | 河南科技大学东海硅产业节能技术研究院 | Wireless temperature measuring device of rotary mechanical body |
| WO2016136919A1 (en) * | 2015-02-25 | 2016-09-01 | 株式会社山本金属製作所 | Temperature measurement device |
| CN108254102A (en) * | 2017-11-30 | 2018-07-06 | 北京原力辰超导技术有限公司 | A kind of high temperature superconductor coil fever detection device |
| CN108279124A (en) * | 2018-01-24 | 2018-07-13 | 常州克劳诺斯特种轴承制造有限公司 | Moving-coil detects bearing |
| CN209310946U (en) * | 2018-09-28 | 2019-08-27 | 北京金豪制药股份有限公司 | A kind of temperature measuring equipment based on wireless power |
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