CN108981597A - A kind of inhibition zone automatic measuring instrument - Google Patents
A kind of inhibition zone automatic measuring instrument Download PDFInfo
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- CN108981597A CN108981597A CN201810724770.9A CN201810724770A CN108981597A CN 108981597 A CN108981597 A CN 108981597A CN 201810724770 A CN201810724770 A CN 201810724770A CN 108981597 A CN108981597 A CN 108981597A
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- 230000005764 inhibitory process Effects 0.000 title claims abstract description 27
- 238000012545 processing Methods 0.000 claims abstract description 35
- 238000007781 pre-processing Methods 0.000 claims abstract description 28
- 239000004973 liquid crystal related substance Substances 0.000 claims abstract description 16
- 238000005259 measurement Methods 0.000 claims abstract description 15
- 238000003860 storage Methods 0.000 claims abstract description 14
- 230000003385 bacteriostatic effect Effects 0.000 claims description 33
- 241000894006 Bacteria Species 0.000 claims description 15
- 239000003814 drug Substances 0.000 claims description 12
- 229940079593 drug Drugs 0.000 claims description 11
- 238000003708 edge detection Methods 0.000 claims description 9
- 238000001914 filtration Methods 0.000 claims description 8
- 239000011324 bead Substances 0.000 claims description 6
- 230000001502 supplementing effect Effects 0.000 claims description 5
- 238000000034 method Methods 0.000 description 13
- 239000003242 anti bacterial agent Substances 0.000 description 8
- 229940088710 antibiotic agent Drugs 0.000 description 8
- 230000003115 biocidal effect Effects 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 230000009466 transformation Effects 0.000 description 4
- 238000004364 calculation method Methods 0.000 description 3
- 230000002401 inhibitory effect Effects 0.000 description 3
- 238000007500 overflow downdraw method Methods 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 230000002147 killing effect Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 241000192125 Firmicutes Species 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000013500 data storage Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000840 electrochemical analysis Methods 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 238000013048 microbiological method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 238000001945 resonance Rayleigh scattering spectroscopy Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01B—MEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
- G01B11/00—Measuring arrangements characterised by the use of optical techniques
- G01B11/08—Measuring arrangements characterised by the use of optical techniques for measuring diameters
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01B—MEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
- G01B11/00—Measuring arrangements characterised by the use of optical techniques
- G01B11/28—Measuring arrangements characterised by the use of optical techniques for measuring areas
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T7/00—Image analysis
- G06T7/0002—Inspection of images, e.g. flaw detection
- G06T7/0012—Biomedical image inspection
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- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
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Abstract
A kind of inhibition zone automatic measuring instrument, including image capture module, image pre-processing module, image processing module, image display, and image storage module, image capture module and image storage module are connect with image pre-processing module, image pre-processing module is connect with image processing module, image processing module is connect with image display, the image capture module includes video camera, image pre-processing module includes filter, image processing module includes DSP signal processor, image display includes liquid crystal display, image storage module includes memory.The instrument is special object special equipment, structure is more compact, portability is more preferable, the automatic measurement of inhibition zone can be completed without debugging and installing various softwares in the instrument simultaneously, reduce the measurement threshold of inhibition zone, a variety of image processing algorithms are merged, reduce the measurement error of inhibition zone further, measurement result is more accurate.
Description
Technical Field
The invention relates to the technical field of medical experiment inspection, in particular to an automatic antibacterial zone measuring instrument.
Background
Certain microorganisms have an inhibitory effect on the growth and reproduction of other microorganisms, and this phenomenon is called antibiotics, and these microorganisms and their preparations are called antibiotics. The antibiotic has good killing and inhibiting effects on various infections caused by gram-positive bacteria, gram-negative bacteria and the like, and is widely applied to the aspects of clinic, veterinary medicine, agriculture and the like. While antibiotics are widely used, the harm of the residues of the antibiotics in animal food to human bodies is attracting more and more attention.
Currently, methods for measuring antibiotics include microbiological methods, chromatographic methods, spectrophotometric methods, chemiluminescence analysis methods, immunoassay methods, resonance rayleigh scattering methods, electrochemical analysis methods, and the like. Wherein, the microbiological assay method of the antibiotic potency, the tube-disc method and the drug sensitive paper sheet method are the general methods of the antibiotic potency at home and abroad at present. The tube-disc method is to utilize the diffusion of antibiotics in the agar culture medium for culturing specific experiments to form a circular area containing antibiotics for inhibiting the propagation of experimental bacteria or killing the experimental bacteria. The action mechanism of different antibiotics for different experimental bacteria is different, and no matter sterilization or bacteria growth inhibition, a very obvious inhibition zone can be observed through an agar culture medium. The larger the zone of inhibition, the stronger the bacteriostatic action of the drug, and the antibiotic titer can be determined by measuring the size of the zone of inhibition of the microorganism.
The traditional bacteriostatic circle measuring method is to use a vernier caliper to carry out manual non-contact measurement on the back of a culture dish, has low precision and large subjectivity, and consumes a great deal of energy of experimenters when measuring a plurality of samples. The special bacteriostatic circle measuring instrument using the optical non-contact measuring method has high price and cannot ensure the measuring precision. The existing bacteriostatic circle measuring instrument has the following defects: 1. the device is connected with a computer through a data line, and the antibacterial zone can be measured after related software is installed on the computer, the measuring instrument requires higher requirements of a tester on equipment installation, program installation, debugging and the like, and the use threshold of the tester is increased; 2. although the pixels of the bacteriostatic zone shot by the CCD camera are higher than those shot by the CMOS camera, the cost is 2 times or more of that of a bacteriostatic zone image shot by the CMOS camera, so that the test cost is greatly increased, and the bacteriostatic zone image scanned by the scanner has overlarge distortion degree, so that the area of the measured bacteriostatic zone has great deviation from the actual size; 3. the image processing module adopts a single algorithm, so that the noise of the bacteriostatic zone image is more, the anti-interference is poor, and the detection efficiency is low.
Disclosure of Invention
In order to solve the problems, the invention designs the automatic measuring instrument for the bacteriostatic circle, the instrument is special equipment for special objects, the structure is more compact, the portability is better, meanwhile, the instrument can complete the automatic measurement of the bacteriostatic circle without debugging and installing various software, and the measuring threshold of the bacteriostatic circle is reduced; the instrument improves the traditional single image processing algorithm, integrates multiple image processing algorithms, further reduces the measurement error of the inhibition zone, and has more accurate measurement result.
In order to achieve the technical purpose and achieve the technical effects, the invention is realized by the following technical scheme:
the automatic measuring instrument for the bacteriostatic circle is characterized by comprising the following modules:
the image acquisition module is used for shooting a bacteriostatic zone on the fastidious bacteria drug sensitive flat plate into an electronic picture format and transmitting the electronic picture format to the image preprocessing module;
the image preprocessing module is used for carrying out graying, filtering and edge detection on the electronic picture of the bacteriostatic zone transmitted by the image acquisition module to obtain a smooth electronic picture of the bacteriostatic zone;
the image processing module is used for carrying out least square fitting on the smooth electronic picture of the inhibition zone obtained by preprocessing to realize automatic measurement of the inhibition zone;
the image display module is used for displaying the measured electronic picture of the inhibition zone;
the image storage module is used for storing the data of the smooth electronic picture of the inhibition zone obtained by preprocessing so as to lead out the data conveniently;
the image acquisition module and the image storage module are connected with the image preprocessing module, the image preprocessing module is connected with the image processing module, and the image processing module is connected with the image display module.
Furthermore, the image preprocessing module adopts a weighted average value method when carrying out gray processing on the electronic picture of the inhibition zone, the filtering adopts a median filtering method, and the edge detection adopts a Sobel operator and a wavelet transformation data fusion method.
Furthermore, the image acquisition module comprises a camera, the image preprocessing module comprises a filter, the image processing module comprises a DSP signal processor, the image display module comprises a liquid crystal display, and the image storage module comprises a memory.
Further, the device comprises an automatic measuring box, wherein the upper part of the automatic measuring box is provided with an image processing measuring box, the lower part of the automatic measuring box is provided with a picture shooting box, the image processing measuring box and the picture shooting box are integrated, a liquid crystal display is fixed on a front panel of the image processing measuring box, an operation key is arranged below the liquid crystal display, a printing interface, a reading interface and a power plug are arranged on a right panel of the image processing measuring box, the printing interface and the reading interface are USB3.0 interfaces, the inside of the picture shooting box is hollow, the front side of the picture shooting box is provided with a shading box door, a camera is fixed on a fixed seat on a top plate of the picture shooting box, a light supplementing lamp bead is arranged on one circle of the periphery of the fixed seat, a shooting table is fixed on a bottom plate of the picture shooting box, the top surface of the shooting table is provided with a fastidious bacteria drug sensitive flat plate fixing groove, the sliding groove of the shooting table is embedded with the sliding rail and can slide along the sliding rail, and handles are arranged on the shading box door and the shooting table.
Furthermore, the camera of the camera is a CMOS camera, the filter is a median filter, the DSP signal processor selects a TMS320F2812 chip produced by Texas instruments, and the liquid crystal display selects an LCD.
The invention has the beneficial effects that: the instrument is special equipment, has a more compact structure and better portability, can finish automatic measurement of the bacteriostatic circle without debugging and installing various software, and reduces the measurement threshold of the bacteriostatic circle; the instrument improves the traditional single image processing algorithm, integrates multiple image processing algorithms, further reduces the measurement error of the inhibition zone, and has more accurate measurement result.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a functional structure block diagram of an automatic bacteriostatic circle measuring instrument;
FIG. 2 is a block diagram of an automatic measuring instrument for bacteriostatic circle;
FIG. 3 is a schematic perspective view of the automatic measuring chamber;
FIG. 4 is a schematic view of the internal structure of the automatic measuring box;
fig. 5 is a schematic view of the fixed structure of the camera.
In the drawings, the components represented by the respective reference numerals are listed below:
1-automatic measuring box, 2-picture shooting box, 3-light supplement lamp bead, 11-liquid crystal display, 12-operation key, 13-printing interface, 14-reading interface, 15-power plug, 16-DSP signal processor, 21-light shading box door, 22-camera, 23-shooting table, 24-handle, 101-image acquisition module, 102-image preprocessing module, 103-image processing module, 104-image display module, 105-image storage module, 161-filter, 162-memory, 201-slide rail, 202-top plate, 221-fixed seat, 231-fastidious bacteria drug sensitive plate fixed groove.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-5, an automatic measuring apparatus for bacteriostatic circle comprises the following modules:
the image acquisition module is used for shooting a bacteriostatic zone on the fastidious bacteria drug sensitive flat plate into an electronic picture format and transmitting the electronic picture format to the image preprocessing module;
the image preprocessing module is used for carrying out graying, filtering and edge detection on the electronic picture of the bacteriostatic zone transmitted by the image acquisition module to obtain a smooth electronic picture of the bacteriostatic zone;
the image processing module is used for carrying out least square fitting on the smooth electronic picture of the inhibition zone obtained by preprocessing to realize automatic measurement of the inhibition zone;
the image display module is used for displaying the measured electronic picture of the inhibition zone;
the image storage module is used for storing the data of the smooth electronic picture of the inhibition zone obtained by preprocessing so as to lead out the data conveniently;
the image acquisition module and the image storage module are connected with the image preprocessing module, the image preprocessing module is connected with the image processing module, and the image processing module is connected with the image display module.
The image preprocessing module adopts a weighted average value method when carrying out gray processing on the electronic picture of the inhibition zone, the filtering adopts a median filtering method, and the edge detection adopts a Sobel operator and a wavelet transformation data fusion method; wherein,
the weighted average method has the calculation formula ofIn the formula WR、WG、WBR, G, B respectively, and the optimal values are 0.23, 0.59 and 0.18 respectively; scanning a window W on an image, sequencing the gray values of all points contained in the window, taking the central gray value as the gray value of the center of the window, wherein the calculation formula is g (m, n) ═ med { f (m-k, n-l), (k, l) ∈ W };
the Sobel operator is an edge detection operator of first order differential, which takes an image to be detected as a curved surface, takes the Sobel edge detection operator as a gradient operator, acts on the image f (x, y), and can obtain a vector fieldLocal gradient strengthAnd local gradient direction
The wavelet transform has good local or property in time domain and frequency domain, and adopts step-by-step fine time domain or frequency domain sampling step size for high-frequency component, so that it can focus on arbitrary details of image, and its formula isWherein a is a frequency parameter and b is a time offset;
the Sobel operator and wavelet transformation data fusion method comprises the following steps:
(1) acquiring the n-th frame of real-time image from the preprocessed image, and recording the n-th frame of real-time image as (f)1,f2,…fn);
(2) Extracting a gray image and simultaneously sending the gray value into Sobel and wavelet transformation for edge detection;
(3) the edges detected by the two algorithms are fused, namely the obtained edge data are overlapped, the image edge after being overlapped is clearer, and meanwhile, the background noise of the image is inhibited to a great extent.
The image acquisition module 101 includes the camera 22, the image preprocessing module 102 includes the filter 161, the image processing module 103 includes the DSP signal processor 16, the image display module 104 includes the liquid crystal display 11, and the image storage module 105 includes the memory 162.
Wherein, the device also comprises an automatic measuring box 1, the upper part of the automatic measuring box 1 is an image processing measuring box, the lower part of the automatic measuring box is a picture shooting box 2, the image processing measuring box and the picture shooting box 2 are integrated box bodies, a liquid crystal display 11 is fixed on the front panel of the image processing measuring box, an operation key 12 is arranged below the liquid crystal display 11, a printing interface 13, a reading interface 14 and a power plug 15 are arranged on the right panel of the image measuring box, the inside of the picture shooting box 2 is hollow, the front side of the picture shooting box is provided with a shading box door 21, a camera 22 is fixed on a fixed seat 221 on the top plate of the picture shooting box, a light supplementing lamp bead 3 is arranged on the periphery of the fixed seat 221, a shooting platform 23 is fixed on the bottom plate of the picture shooting platform 23, a fastly-cultured bacteria and drug sensitive flat plate fixed groove 231 is arranged on the, the bottom surface of the picture shooting box is provided with a slide rail 201, the slide groove of the shooting platform 23 is embedded and connected with the slide rail 201 and can slide along the slide rail 201, and the shading box door 21 and the shooting platform 23 are provided with handles 24.
The camera of the camera 22 is a CMOS camera, the filter 161 is a median filter, the DSP signal processor 16 selects a TMS320F2812 chip produced by texas instruments, and the liquid crystal display 11 selects an LCD liquid crystal display.
One specific application of the device is as follows: the light shielding box door 21 is opened, the shooting table 23 is pulled out of the picture shooting box 2, the fastidious bacteria and drug sensitive flat plate is flatly placed in the fastidious bacteria and drug sensitive flat plate fixing groove 231, then the sliding groove of the shooting table 23 is matched with the sliding rail 201 on the bottom of the picture shooting box and slides into the picture shooting box 2, and the light shielding box door 21 is closed to keep a dark environment in the picture shooting box 2; the power plug 15 is connected with a power supply and a power switch button is pressed, the light supplementing lamp beads 3 provide illumination for the camera 22 to take pictures, and because the light supplementing lamp beads 3 are annularly arranged along the camera 22, shadowless illumination can be provided, so that high-quality images can be obtained through shooting; when the working button is pressed, the camera 22 transmits the shot picture to a median filter for graying, filtering and edge detection to obtain a smooth electronic picture of the bacteriostatic circle, and the preprocessed electronic picture of the bacteriostatic circle is input into the DSP signal processor 16 for least square fitting calculation to obtain the diameter of the bacteriostatic circle and the area of the bacteriostatic circle; the automatically measured picture is displayed through the liquid crystal display 11; the printer device is connected with the automatic measuring box 1 through the printing interface 13, and the measured picture can be printed in real time for analysis by a detector by pressing a printing button; the DSP signal processor 16 transmits the processed picture information to the memory 162 for data storage; the inspector can connect the mobile storage device to the automatic measuring box 1 through the USB data cable to read the data picture stored in the memory 162.
In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.
Claims (4)
1. The automatic measuring instrument for the bacteriostatic circle is characterized by comprising the following modules:
the image acquisition module is used for shooting a bacteriostatic zone on the fastidious bacteria drug sensitive flat plate into an electronic picture format and transmitting the electronic picture format to the image preprocessing module;
the image preprocessing module is used for carrying out graying, filtering and edge detection on the electronic picture of the bacteriostatic zone transmitted by the image acquisition module to obtain a smooth electronic picture of the bacteriostatic zone;
the image processing module is used for carrying out least square fitting on the smooth electronic picture of the inhibition zone obtained by preprocessing to realize automatic measurement of the inhibition zone;
the image display module is used for displaying the measured electronic picture of the inhibition zone;
the image storage module is used for storing the data of the smooth electronic picture of the inhibition zone obtained by preprocessing so as to lead out the data conveniently;
the image acquisition module and the image storage module are connected with the image preprocessing module, the image preprocessing module is connected with the image processing module, and the image processing module is connected with the image display module.
2. The automatic bacteria inhibition zone measuring instrument according to claim 1, wherein the image acquisition module comprises a camera, the image preprocessing module comprises a filter, the image processing module comprises a DSP signal processor, the image display module comprises a liquid crystal display, and the image storage module comprises a memory.
3. The automatic measuring instrument for bacteriostatic circle according to claim 2, characterized by further comprising an automatic measuring box, wherein the upper part of the automatic measuring box is an image processing measuring box, the lower part of the automatic measuring box is a picture shooting box, the image processing measuring box and the picture shooting box are an integrated box body, the liquid crystal display is fixed on the front panel of the image processing measuring box, the operating keys are arranged below the liquid crystal display, the right panel of the image measuring box is provided with a printing interface, a reading interface and a power plug, the inside of the picture shooting box is hollow, the front side of the picture shooting box is provided with a shading box door, the camera is fixed on a fixed seat on the top plate of the picture shooting box, the periphery of the fixed seat is provided with a light supplementing lamp bead, the bottom plate of the picture shooting box is fixed with a shooting table, the top surface of the shooting table is provided with a fastidious bacteria drug sensitive flat plate fixing, the bottom plate surface of the picture shooting box is provided with a slide rail, a slide groove of the shooting platform is embedded with the slide rail and can slide along the slide rail, and handles are arranged on the shading box door and the shooting platform.
4. The automatic measuring instrument for the inhibition zone according to claim 2, wherein the camera of the camera is a CMOS camera, the filter is a median filter, the DSP signal processor is a TMS320F2812 chip produced by texas instruments, and the LCD is a liquid crystal display.
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Application publication date: 20181211 |
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RJ01 | Rejection of invention patent application after publication |