CN108967530A - A kind of apple fresh-keeping liquid and its preparation method and application - Google Patents
A kind of apple fresh-keeping liquid and its preparation method and application Download PDFInfo
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/16—Coating with a protective layer; Compositions or apparatus therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to a kind of apple fresh-keeping liquid and its preparation method and application, it is in 1%-5% acetum that active principle, which is dissolved in volumetric concentration, the mass concentration being configured to is the sticky colloidal solution of 10-30%, active principle comprises the following components in parts by weight: 15~30 parts of shenqinmycin ferment filtrate, 5~10 parts of aubergine pigment extracting solution, 10~20 parts of chitosan, 5~10 parts of cornstarch, 15~30 parts of distilled water.Compared with prior art, safe green environmental protection of the present invention, instrument and equipment is simple, agents useful for same is cheap and easy to get, convenient for the popularization and application of industrialization production and market, and the security hidden troubles such as the residual, toxicity of agriculture are resolved, it can be fresh-keeping for a long time without chemical pesticide or preservative.
Description
Technical field
The present invention relates to fruit freshness preserving technical fields, more particularly, to a kind of apple fresh-keeping liquid and preparation method thereof and answer
With.
Background technique
Apple is spherical, and nature and flavor are mild, crisp, and carbohydrate rich in, vitamin and micro member
Element, organic acid, pectin, protein, calcium, phosphorus, potassium, iron, vitamin A, vitamin B, vitamin C and dietary fiber, separately contain apple
Tartaric acid, tartaric acid, carrotene are that nutritive value thus has " king of fruit " closest to perfect one in all vegetables and fruits
Title.China is the maximum apple production state in the whole world, and yield accounts for 50% or more of global apple production, and Apple Export occupies the world
Forefront.China's apple production in 2016 is 43,880,000 tons, is the maximum fruit variety of China's yield (without melon).But due to apple
Fruit production have certain seasonality and regionality, and fresh-retaining preserving condition limit, it occur frequently that nutrition loss, loss of water and
Phenomena such as rotting be in developed countries such as the U.S., Japan in transport and the rotten loss late of storage period apple according to statistics
15%~25%, and China is then up to 30%~40%.Therefore, apple fresh-keeping is paid attention to by domestic Many researchers and orchard worker,
It is particularly important to find simple and effective preservation method.
The main reason for leading to apple decay is: fruit itself breathes active, the dematuration of ethylene, pathogen taste
Raw and epidermis the problems such as damaging of colliding with, wherein especially the most prominent with the growth of pathogen.Because of the average moisture content of apple
60% or more, the apple water content of fresh picking is even as high as 90%, and carbohydrate average content is 10%~15%, egg
The content of other nutriments such as white matter, fat is 0.5%~1%, and for nutritional ingredient, apple can be bacterium and fungi
Offer condition is grown, but its pH value is lower than the pH value that most of bacteriums can grow, therefore apple decay is mainly drawn by fungi
It rises.After fungal infection fruit, consuming organic acid by carbohydrate metabolism rises the pH of fruit, is further bacterium
Growth creates conditions, therefore the growth of control fungi is essentially consisted in the control of apple decay.
Currently, apple fresh-keeping method is broadly divided into three physics, chemistry and biological method major class.Physical method such as freezer is low
Temperature storage, air conditioned storage and subatmospheric pressure storage etc. cannot be widely applied since technical requirements are high, cost input is big, and
Its in terms of controlling fungi growth without obvious effect, usually as a kind of supplementary means.Chemical method such as preservative, insecticide
Deng the problems such as since its chemical residual is serious, there are security risks, are also easy to produce drug resistance strain, and environmental pollution is serious, so that disappearing
The person of expense is more and more sensitive to the use of chemical agent, and many countries propose limitation and use to various chemical medicaments even to be prohibited
Only use.Biological method is a kind of preservation method emerging in recent years, it is to utilize antagonism beneficial bacterium or biocidal property metabolite
A kind of preservation technology for carrying out anti-corrosive fresh-keeping, is a kind of more safe and nontoxic, free of contamination environmentally friendly preservation method.
But studying at present more is fresh-keeping liquid that other organic or inorganic objects such as Chinese medicinal plants extracting solution such as ginger mix, leaching
Pickled apple progress is fresh-keeping, and generally existing pesticide residue is high at present, drug effect is low, narrow antimicrobial spectrum, extraction cost for China's Chinese medicinal plant
High problem, therefore one kind there is no effectively to inhibit fungi growth and safety and environmental protection, apple fresh-keeping side simple to operation in the market
Method.
Summary of the invention
It is an object of the present invention to overcome the above-mentioned drawbacks of the prior art and provide a kind of apple fresh-keeping liquid and
Preparation method and application.
The purpose of the present invention can be achieved through the following technical solutions:
A kind of apple fresh-keeping liquid, it is the quality that is configured in 1%-5% acetum that active principle, which is dissolved in volumetric concentration,
Concentration is the sticky colloidal solution of 10-30%,
Preferably, active principle comprises the following components in parts by weight: 15~30 parts of shenqinmycin ferment filtrate, aubergine pigment mentions
Take 5~10 parts of liquid, 10~20 parts of chitosan, 5~10 parts of cornstarch.
Preferably, active principle comprises the following components in parts by weight: 18~25 parts of shenqinmycin ferment filtrate, aubergine pigment mentions
Take 6~8 parts of liquid, 10~20 parts of chitosan, 5~8 parts of cornstarch.
Preferably, active principle comprises the following components in parts by weight: 20~25 parts of shenqinmycin ferment filtrate, aubergine pigment mentions
Take 6~8 parts of liquid, 12~18 parts of chitosan, 6~8 parts of cornstarch.
Preferably, the shenqinmycin ferment filtrate is that pseudomonas fluorescens M18 extracts after deep liquid ventilating fermentation
Resulting ferment filtrate after shenqinmycin, which takes supernatant after ultrasound, centrifugation, to remove extra fermentation impurities and bacterium
Body residue.
It is further preferred that the pseudomonas fluorescens M18 is commonly micro- by China Committee for Culture Collection of Microorganisms
Bio-Centers preservation, deposit number: CGMCC.NO.0462, in Chinese patent CN1141379C, patent name is biological pesticide rush
Into being disclosed in Antagonistic Fungi M18 and preparation method thereof.
It is further preferred that the frequency of ultrasonic treatment is 800kHZ, the revolving speed of time 30min, centrifugal treating are
3000rpm, time 5min, obtained supernatant detect its shenqinmycin content in 24.8~32.8mg/L through HPLC.
Preferably, the aubergine pigment extracting solution is to extract to obtain by Boiling bath method, controls 1~5 part of aubergine pigment, anhydrous second
80~100 parts of alcohol, 60min is reacted in boiling water bath, is filtered, supernatant liquor is aubergine pigment extracting solution.
Preferably, chitosan chemical name is Chitosan (1-4) -2- amino-B-D glucose, can be bought from upper
Extra large Chinese medicines group.
Preferably, the cornstarch is also known as corn starch, popular name maize powder, the white flaxen powder of micro-strip,
It can buy from Shanghai Aladdin biochemical technology limited liability company.
Preferably, the volumetric concentration of the acetum is 1%-3%, can be bought from Shanghai Chinese medicines group.
The preparation method of apple fresh-keeping liquid, using following steps:
(1) it dissolves the chitosan in acetum, is configured to chitosan solution；
(2) cornstarch is dissolved in acetum, is heated to 80 DEG C, is configured to corn starch solution；
(3) shenqinmycin ferment filtrate is added after mixing chitosan solution and corn starch solution in equal volume, stirring is equal
It is even；
(4) aubergine pigment extracting solution is added in above-mentioned mixed liquor and carries out continuing stirring using same direction, it is equal to solution
To get apple fresh-keeping liquid after matter.
The application of apple fresh-keeping liquid, using following steps:
(1) selected epidermis knocks down fresh-keeping medical fluid without the apple damaged, no disease and pests harm infects, and submerges 5~15min, then fishes out
Get up, pays attention to guaranteeing that carpopodium will soak；
(2) after having soaked medical fluid, it is packed into ventilation water tank, air-dries the medical fluid of fruit surface；
(3) apple after air-drying being packed into plastic film single fruit freshness protection package, sack is tightened, 4 DEG C of low-temperature cold store storages are put into,
After being stored apple 7 months by this method, healthy fruit 90% or so, carpopodium is dark green, and fruit is fresh and sweet as before.
Compared with prior art, the invention has the following advantages that
1. shenqinmycin ferment filtrate and aubergine pigment extracting solution in apple fresh-keeping method of the present invention, are current
By-product in industrial production not only solves industrial pair by the re-using after the method for the invention simple process
The subsequent processing problem of product also provides new approaches for apple fresh-keeping research.
2. shenqinmycin ferment filtrate of the present invention, after simple process, shenqinmycin content 24.8~
32.8mg/L.This is a kind of microbial source fungicide efficiently, less toxic, environment compatibility is good, can inhibit various bacteria and fungi
Venereal disease evil, and to human body and animal safety.Inhibit the growth of the pathogens such as penicillium expansum with shenqinmycin, can effectively solve
The low problem of narrow antimicrobial spectrum, drug effect in preservation method at present, and it is natural products safety and environmental protection, to human body and animal without
Evil.In the present invention, to the ultrasonic centrifugal treating of shenqinmycin ferment filtrate, can effectively remove fermentation impurities in filtrate and
Thallus residue, other injuries will not be caused to apple by impregnating apple with it.
3. bacteria breed can not only be inhibited containing substances such as polyphenol, anthocyanin in aubergine pigment extracting solution of the present invention,
Also have the function of removing DPPH free radical and hydroxy radical.The respiratory activity of apple is very active, and constantly breathing obtains
Oxygen, oxygen is necessary to carrying out each kinds of oxidation reaction, however when utilizing the oxygen certain metabolic responses of progress, inevitably
Some unstable materials can be generated, and these unstable substances are all oxygen " variable bodies ", their individual character is very active, no
Like staying in original place with being firm and secure, or even like attacking other people, is not liked since they are this by contained individual character, referred to as
For free radical.Cell and tissue breakdown can be influenced metabolic function by free radical or oxidant, and can be caused different damages and be broken
It is bad.If excessive oxyradical can be eliminated, cause injury that can prevent many free radicals.Therefore, aubergine pigment
Extracting solution has vital effect during apple fresh-keeping.
4., because of chitosan, being unique a kind of alkali in nature currently on the market using preferably at most being formed a film with chitosan
Property polysaccharide, be to obtain by chitosan moiety or after acetyl group is totally removed, there is very high viscosity, good water imbibition, guarantor
Moist, film forming, thickening property, gelation etc..In addition, chitosan is present in nature in large quantities, and nontoxic, it is degradable,
It is a kind of reproducible resource.After wrapping apple, since transmission ability of the chitosan film to carbon dioxide is poor, titanium dioxide
Carbon content increases, and oxygen content reduces, so that respiratory intensity decline is controlled, and film has good barrier property to vapor, it can
To prevent the evaporation of moisture in apple.But the fruit antistaling agent directly made using chitosan and its derivative as raw material, due to
It is with water imbibition, if may damage to plants caused by sudden drop in temperature due to absorbing moisture to the apple generation in refrigeration without processing.Therefore, this hair
It is bright and be added to cornstarch, it is commonly called as maize powder, there is very strong hygroscopicity, to can be reduced chitosan water imbibition to the danger of apple
Evil, and cornstarch is used in biological preservation film, the viscosity of plastics can be remarkably reinforced, increase encapsulation ratio, reduce film
Duck eye can significantly improve fresh-keeping effect.Chitosan and cornstarch in fresh-keeping liquid of the present invention, apple is through of short duration immersion
Afterwards, it can be formed on its surface one layer of biomembrane, air can not only be completely cut off, moreover it is possible to effectively prevent the evaporation of apple moisture.Join herein
It examines patent of invention " a kind of garlic fresh-keeping film-coating agent and preparation method thereof ", which is mainly used for that garlic is fresh-keeping, and the present invention is right
It has carried out being added to the antimycotic biological pesticide Shen of special efficacy by specially treated for the fresh-keeping of apple after improving and innovating
Piperazine mycin ferment filtrate and aubergine pigment extracting solution with obvious antioxidation.
5. shenqinmycin ferment filtrate, aubergine pigment extracting solution used in the present invention, chitosan, cornstarch are naturally to come
The organic matter in source, toxicity relative to small more and degradable of artificial chemistry synthetic, with it impregnates apple, and there is no residuals
The problems such as high, security risks such as toxicity is big and environmental pollution, can be fresh-keeping for a long time without chemical pesticide or preservative, it is fresh-keeping
Method safety is environmentally protective, and instrument and equipment is simple, agents useful for same is cheap and easy to get, and the popularization convenient for industrialization production and market is answered
With.
6. shenqinmycin ferment filtrate and aubergine pigment extracting solution used in the present invention, realize recycling for resource.
Specific embodiment
The present invention is described in detail combined with specific embodiments below.Following embodiment will be helpful to the technology of this field
Personnel further understand the present invention, but the invention is not limited in any way.It should be pointed out that the ordinary skill of this field
For personnel, without departing from the inventive concept of the premise, various modifications and improvements can be made.These belong to the present invention
Protection scope.
Embodiment 1
(1) 15 parts of chitosan are dissolved in 1% acetum, are configured to chitosan solution；
(2) 5 parts of cornstarch are dissolved in 1% acetum, are heated to 80 DEG C, it is molten to be configured to cornstarch by 5min
Liquid；
(3) 15 parts of shenqinmycin ferment filtrates are added after mixing chitosan solution and corn starch solution in equal volume, stir
It mixes uniformly；
(4) 5 parts of aubergine pigment extracting solutions are added in above-mentioned mixed liquor, stirring while adding, stirring should be the same direction, to
To get apple fresh-keeping liquid after solution homogeneous.
(5) selected epidermis is knocked down fresh-keeping without the apple (Aksu of Xinjiang 70~75mm of fruit diameter) damaged, no disease and pests harm infects
Medical fluid submerges 8min, then gets, and pays attention to guaranteeing that carpopodium will soak.
(6) after having soaked medical fluid, it is packed into ventilation water tank, air-dries the medical fluid of fruit surface.
(7) apple after air-drying being packed into plastic film single fruit freshness protection package, sack is tightened, 4 DEG C of low-temperature cold store storages are put into,
After being stored apple 6 months by this method, healthy fruit 90% or so, carpopodium is dark green, and fruit is fresh and sweet as before.
Embodiment 2
(1) 20 parts of chitosan are dissolved in 2% acetum, are configured to chitosan solution；
(2) 8 parts of cornstarch are dissolved in 2% acetum, are heated to 80 DEG C, it is molten to be configured to cornstarch by 5min
Liquid；
(3) 25 parts of shenqinmycin ferment filtrates are added after mixing chitosan solution and corn starch solution in equal volume, stir
It mixes uniformly；
(4) 8 parts of aubergine pigment extracting solutions are added in above-mentioned mixed liquor, stirring while adding, stirring should be the same direction, to
To get apple fresh-keeping liquid after solution homogeneous.
(5) selected epidermis is knocked down without the apple (Shandong Yantai 70~75mm of Fuji apple's fruit diameter) damaged, no disease and pests harm infects
Fresh-keeping medical fluid submerges 10min, then gets, and pays attention to guaranteeing that carpopodium will soak.
(6) after having soaked medical fluid, it is packed into ventilation water tank, air-dries the medical fluid of fruit surface.
(7) apple after air-drying being packed into plastic film single fruit freshness protection package, sack is tightened, 4 DEG C of low-temperature cold store storages are put into,
After being stored apple 8 months by this method, healthy fruit 85% or so, carpopodium is dark green, and fruit is fresh and sweet as before.
Embodiment 3
(1) 20 parts of chitosan are dissolved in 3% acetum, are configured to chitosan solution；
(2) 10 parts of cornstarch are dissolved in 3% acetum, are heated to 80 DEG C, it is molten to be configured to cornstarch by 5min
Liquid；
(3) 30 parts of shenqinmycin ferment filtrates are added after mixing chitosan solution and corn starch solution in equal volume, stir
It mixes uniformly；
(4) 10 parts of aubergine pigment extracting solutions are added in above-mentioned mixed liquor, stirring while adding, stirring should be the same direction,
To get apple fresh-keeping liquid after solution homogeneous.
(5) selected epidermis knocks down guarantor without the apple (Luochuan Fuji apple, 70~75mm of fruit diameter) damaged, no disease and pests harm infects
Fresh medicine liquid submerges 15min, then gets, and pays attention to guaranteeing that carpopodium will soak.
(6) after having soaked medical fluid, it is packed into ventilation water tank, air-dries the medical fluid of fruit surface.
(7) apple after air-drying being packed into plastic film single fruit freshness protection package, sack is tightened, 4 DEG C of low-temperature cold store storages are put into,
After being stored apple 10 months by this method, healthy fruit 70% or so, carpopodium is dark green, and fruit is fresh and sweet as before.
Embodiment 4
(1) 15 parts of chitosan are dissolved in 1% acetum, are configured to chitosan solution；
(2) 5 parts of cornstarch are dissolved in 1% acetum, are heated to 80 DEG C, it is molten to be configured to cornstarch by 5min
Liquid；
(3) 20 parts of shenqinmycin ferment filtrates are added after mixing chitosan solution and corn starch solution in equal volume, stir
It mixes uniformly；
(4) 8 parts of aubergine pigment extracting solutions are added in above-mentioned mixed liquor, stirring while adding, stirring should be the same direction, to
To get apple fresh-keeping liquid after solution homogeneous.
(5) selected epidermis is knocked down without the apple (Tianshui city flower ox, 70~75mm of fruit diameter) damaged, no disease and pests harm infects
Fresh-keeping medical fluid submerges 15min, then gets, and pays attention to guaranteeing that carpopodium will soak.
(6) after having soaked medical fluid, it is packed into ventilation water tank, air-dries the medical fluid of fruit surface.
(7) apple after air-drying being packed into plastic film single fruit freshness protection package, sack is tightened, 4 DEG C of refrigerator storages are put into, every
Record its moisture content, respiratory intensity, hardness and pol within seven days.
1 Tianshui city of table flower ox apple impregnates fresh-keeping each index variation
Table 1 is after the fresh-keeping liquid that the present embodiment is prepared is to apple processing, and each fresh-keeping index changes with time
Situation, as can be seen from Table 1 with the extension of fresh keeping time, every organoleptic indicator's amplitude of variation of flower ox apple is smaller and smaller,
Therefore loss of water can be reduced with preservation method described in indirect reaction embodiment, inhibits respiratory intensity, increase pol.
Embodiment 5
(1) 20 parts of chitosan are dissolved in 3% acetum, are configured to chitosan solution；
(2) 10 parts of cornstarch are dissolved in 3% acetum, are heated to 80 DEG C, it is molten to be configured to cornstarch by 5min
Liquid；
(3) 25 parts of shenqinmycin ferment filtrates are added after mixing chitosan solution and corn starch solution in equal volume, stir
It mixes uniformly；
(4) 10 parts of aubergine pigment extracting solutions are added in above-mentioned mixed liquor, stirring while adding, stirring should be the same direction,
To get apple fresh-keeping liquid after solution homogeneous.
(5) selected epidermis knocks down guarantor without the apple (Luochuan Fuji apple, 70~75mm of fruit diameter) damaged, no disease and pests harm infects
Fresh medicine liquid submerges 15min, then gets, and pays attention to guaranteeing that carpopodium will soak.
(6) after having soaked medical fluid, it is packed into ventilation water tank, air-dries the medical fluid of fruit surface.
(7) apple after air-drying being packed into plastic film single fruit freshness protection package, sack is tightened, 4 DEG C of refrigerator storages are put into, every
Record its moisture content, respiratory intensity, hardness and pol within seven days.
2 Luochuan red fuji apple of table impregnates fresh-keeping each index variation
Table 2 is after the fresh-keeping liquid that the present embodiment is prepared is to apple processing, and each fresh-keeping index changes with time
Situation, as can be seen from Table 2 with the extension of fresh keeping time, every organoleptic indicator's amplitude of variation of red fuji apple is increasingly
It is small, therefore loss of water can be reduced with preservation method described in indirect reaction embodiment, inhibit respiratory intensity, increases pol
Embodiment 6
(1) 20 parts of chitosan are dissolved in 3% acetum, are configured to chitosan solution；
(2) 10 parts of cornstarch are dissolved in 3% acetum, are heated to 80 DEG C, it is molten to be configured to cornstarch by 5min
Liquid；
(3) 30 parts of shenqinmycin ferment filtrates are added after mixing chitosan solution and corn starch solution in equal volume, stir
It mixes uniformly；
(4) 10 parts of aubergine pigment extracting solutions are added in above-mentioned mixed liquor, stirring while adding, stirring should be the same direction,
To get apple fresh-keeping liquid after solution homogeneous.
(5) selected epidermis is protected without the apple 60 (Luochuan Fuji apple, 70~75mm of fruit diameter) damaged, no disease and pests harm infects
It is suitable to demonstrate,prove fruit matter, there is comparativity.30 are knocked down fresh-keeping medical fluid, are submerged 15min, are then got, and pay attention to guaranteeing that carpopodium will soak；
Remaining 30 are handled as a control group with clear water.
(6) after having soaked medical fluid (or clear water), it is packed into ventilation water tank, air-dries the medical fluid (or clear water) of fruit surface.
(7) apple after air-drying is packed into plastic film single fruit freshness protection package, sack is tightened, and 4 DEG C of low-temperature cold store storages are put into.
5 apples are taken within every 30 days to survey peeling hardness, soluble solid content, weight-loss ratio, the indexs such as Vitamin C content at random.
Result is as follows after 90 days:
3 Luochuan red fuji apple fresh-keeping effect of table compares
Embodiment 7
A kind of apple fresh-keeping liquid, it is the mass concentration that is configured in 1% acetum that active principle, which is dissolved in volumetric concentration,
For 10% sticky colloidal solution,
Wherein, the active principle of use comprises the following components in parts by weight: 15 parts of shenqinmycin ferment filtrate, aubergine pigment mentions
Take 5 parts of liquid, 10 parts of chitosan, 5 parts of cornstarch.
It is mould that the shenqinmycin ferment filtrate used is that pseudomonas fluorescens M18 extracts Shen piperazine after deep liquid ventilating fermentation
Resulting ferment filtrate after element, the filtrate are 3000rpm centrifugal treating 5min through 800kHZ ultrasound 30min, revolving speed, take supernatant
Liquid, to remove extra fermentation impurities and thallus residue, obtained supernatant detects its shenqinmycin content 24.8 through HPLC
~32.8mg/L.Pseudomonas fluorescens M18 is protected by China Committee for Culture Collection of Microorganisms's common micro-organisms center's preservation
Hiding number: CGMCC.NO.0462.
Aubergine pigment extracting solution is to extract to obtain by Boiling bath method, 1 part of aubergine pigment of control, 80 parts of dehydrated alcohol, in boiling water
60min is reacted in bath, is filtered, supernatant liquor is aubergine pigment extracting solution.
The preparation method of apple fresh-keeping liquid, using following steps:
(1) it dissolves the chitosan in acetum, is configured to chitosan solution；
(2) cornstarch is dissolved in acetum, is heated to 80 DEG C, is configured to corn starch solution；
(3) shenqinmycin ferment filtrate is added after mixing chitosan solution and corn starch solution in equal volume, stirring is equal
It is even；
(4) aubergine pigment extracting solution is added in above-mentioned mixed liquor and carries out continuing stirring using same direction, it is equal to solution
To get apple fresh-keeping liquid after matter.
The application of apple fresh-keeping liquid, using following steps:
(1) selected epidermis knocks down fresh-keeping medical fluid without the apple damaged, no disease and pests harm infects, and submerges 5min, then picks up
Come, pays attention to guaranteeing that carpopodium will soak；
(2) after having soaked medical fluid, it is packed into ventilation water tank, air-dries the medical fluid of fruit surface；
(3) apple after air-drying being packed into plastic film single fruit freshness protection package, sack is tightened, 4 DEG C of low-temperature cold store storages are put into,
After being stored apple 7 months by this method, healthy fruit 90% or so, carpopodium is dark green, and fruit is fresh and sweet as before.
Embodiment 8
A kind of apple fresh-keeping liquid, it is the mass concentration that is configured in 3% acetum that active principle, which is dissolved in volumetric concentration,
For 15% sticky colloidal solution,
The active principle used comprises the following components in parts by weight: 18 parts of shenqinmycin ferment filtrate, aubergine pigment extracting solution 6
Part, 10 parts of chitosan, 5 parts of cornstarch.
It is mould that the shenqinmycin ferment filtrate used is that pseudomonas fluorescens M18 extracts Shen piperazine after deep liquid ventilating fermentation
Resulting ferment filtrate after element, the filtrate are 3000rpm centrifugal treating 5min through 800kHZ ultrasound 30min, revolving speed, take supernatant
Liquid, to remove extra fermentation impurities and thallus residue, obtained supernatant detects its shenqinmycin content 24.8 through HPLC
~32.8mg/L.Pseudomonas fluorescens M18 is protected by China Committee for Culture Collection of Microorganisms's common micro-organisms center's preservation
Hiding number: CGMCC.NO.0462.
Aubergine pigment extracting solution is to extract to obtain by Boiling bath method, 3 parts of aubergine pigment of control, 90 parts of dehydrated alcohol, in boiling water
60min is reacted in bath, is filtered, supernatant liquor is aubergine pigment extracting solution.
The preparation method of apple fresh-keeping liquid, using following steps:
(1) it dissolves the chitosan in acetum, is configured to chitosan solution；
(2) cornstarch is dissolved in acetum, is heated to 80 DEG C, is configured to corn starch solution；
(3) shenqinmycin ferment filtrate is added after mixing chitosan solution and corn starch solution in equal volume, stirring is equal
It is even；
(4) aubergine pigment extracting solution is added in above-mentioned mixed liquor and carries out continuing stirring using same direction, it is equal to solution
To get apple fresh-keeping liquid after matter.
The application of apple fresh-keeping liquid, using following steps:
(1) selected epidermis knocks down fresh-keeping medical fluid without the apple damaged, no disease and pests harm infects, and submerges 10min, then picks up
Come, pays attention to guaranteeing that carpopodium will soak；
(2) after having soaked medical fluid, it is packed into ventilation water tank, air-dries the medical fluid of fruit surface；
(3) apple after air-drying being packed into plastic film single fruit freshness protection package, sack is tightened, 4 DEG C of low-temperature cold store storages are put into,
After being stored apple 7 months by this method, healthy fruit 90% or so, carpopodium is dark green, and fruit is fresh and sweet as before.
Embodiment 9
A kind of apple fresh-keeping liquid, it is the mass concentration that is configured in 3% acetum that active principle, which is dissolved in volumetric concentration,
For 20% sticky colloidal solution,
The active principle used comprises the following components in parts by weight: 25 parts of shenqinmycin ferment filtrate, aubergine pigment extracting solution 8
Part, 18 parts of chitosan, 8 parts of cornstarch.
It is mould that the shenqinmycin ferment filtrate used is that pseudomonas fluorescens M18 extracts Shen piperazine after deep liquid ventilating fermentation
Resulting ferment filtrate after element, the filtrate are 3000rpm centrifugal treating 5min through 800kHZ ultrasound 30min, revolving speed, take supernatant
Liquid, to remove extra fermentation impurities and thallus residue, obtained supernatant detects its shenqinmycin content 24.8 through HPLC
~32.8mg/L.Pseudomonas fluorescens M18 is protected by China Committee for Culture Collection of Microorganisms's common micro-organisms center's preservation
Hiding number: CGMCC.NO.0462.
Aubergine pigment extracting solution is to extract to obtain by Boiling bath method, 3 parts of aubergine pigment of control, 90 parts of dehydrated alcohol, in boiling water
60min is reacted in bath, is filtered, supernatant liquor is aubergine pigment extracting solution.
The preparation method of apple fresh-keeping liquid, using following steps:
(1) it dissolves the chitosan in acetum, is configured to chitosan solution；
(2) cornstarch is dissolved in acetum, is heated to 80 DEG C, is configured to corn starch solution；
(3) shenqinmycin ferment filtrate is added after mixing chitosan solution and corn starch solution in equal volume, stirring is equal
It is even；
(4) aubergine pigment extracting solution is added in above-mentioned mixed liquor and carries out continuing stirring using same direction, it is equal to solution
To get apple fresh-keeping liquid after matter.
The application of apple fresh-keeping liquid, using following steps:
(1) selected epidermis knocks down fresh-keeping medical fluid without the apple damaged, no disease and pests harm infects, and submerges 10min, then picks up
Come, pays attention to guaranteeing that carpopodium will soak；
(2) after having soaked medical fluid, it is packed into ventilation water tank, air-dries the medical fluid of fruit surface；
(3) apple after air-drying being packed into plastic film single fruit freshness protection package, sack is tightened, 4 DEG C of low-temperature cold store storages are put into,
After being stored apple 7 months by this method, healthy fruit 90% or so, carpopodium is dark green, and fruit is fresh and sweet as before.
Embodiment 10
A kind of apple fresh-keeping liquid, it is the mass concentration that is configured in 5% acetum that active principle, which is dissolved in volumetric concentration,
For 30% sticky colloidal solution,
The active principle used comprises the following components in parts by weight: 30 parts of shenqinmycin ferment filtrate, aubergine pigment extracting solution
10 parts, 20 parts of chitosan, 10 parts of cornstarch.
It is mould that the shenqinmycin ferment filtrate used is that pseudomonas fluorescens M18 extracts Shen piperazine after deep liquid ventilating fermentation
Resulting ferment filtrate after element, the filtrate are 3000rpm centrifugal treating 5min through 800kHZ ultrasound 30min, revolving speed, take supernatant
Liquid, to remove extra fermentation impurities and thallus residue, obtained supernatant detects its shenqinmycin content 24.8 through HPLC
~32.8mg/L.Pseudomonas fluorescens M18 is protected by China Committee for Culture Collection of Microorganisms's common micro-organisms center's preservation
Hiding number: CGMCC.NO.0462.
Aubergine pigment extracting solution is to extract to obtain by Boiling bath method, 5 parts of aubergine pigment of control, 100 parts of dehydrated alcohol, in boiling water
60min is reacted in bath, is filtered, supernatant liquor is aubergine pigment extracting solution.
The preparation method of apple fresh-keeping liquid, using following steps:
(1) it dissolves the chitosan in acetum, is configured to chitosan solution；
(2) cornstarch is dissolved in acetum, is heated to 80 DEG C, is configured to corn starch solution；
(3) shenqinmycin ferment filtrate is added after mixing chitosan solution and corn starch solution in equal volume, stirring is equal
It is even；
(4) aubergine pigment extracting solution is added in above-mentioned mixed liquor and carries out continuing stirring using same direction, it is equal to solution
To get apple fresh-keeping liquid after matter.
The application of apple fresh-keeping liquid, using following steps:
(1) selected epidermis knocks down fresh-keeping medical fluid without the apple damaged, no disease and pests harm infects, and submerges 15min, then picks up
Come, pays attention to guaranteeing that carpopodium will soak；
(2) after having soaked medical fluid, it is packed into ventilation water tank, air-dries the medical fluid of fruit surface；
(3) apple after air-drying being packed into plastic film single fruit freshness protection package, sack is tightened, 4 DEG C of low-temperature cold store storages are put into,
After being stored apple 7 months by this method, healthy fruit 90% or so, carpopodium is dark green, and fruit is fresh and sweet as before.
Specific embodiments of the present invention are described above.It is to be appreciated that the invention is not limited to above-mentioned
Particular implementation, those skilled in the art can make various deformations or amendments within the scope of the claims, this not shadow
Ring substantive content of the invention.
Claims (9)
1. a kind of apple fresh-keeping liquid, which is characterized in that it is 1%-5% vinegar that active principle is dissolved in volumetric concentration by the apple fresh-keeping liquid
In acid solution, the mass concentration being configured to is the sticky colloidal solution of 10-30%,
The active principle comprises the following components in parts by weight: 15~30 parts of shenqinmycin ferment filtrate, aubergine pigment extracting solution 5
~10 parts, 10~20 parts of chitosan, 5~10 parts of cornstarch.
2. a kind of apple fresh-keeping liquid according to claim 1, which is characterized in that the active principle includes following parts by weight
Component: 18~25 parts of shenqinmycin ferment filtrate, 6~8 parts of aubergine pigment extracting solution, 10~20 parts of chitosan, cornstarch 5~
8 parts.
3. a kind of apple fresh-keeping liquid according to claim 1, which is characterized in that the active principle includes following parts by weight
Component: 20~25 parts of shenqinmycin ferment filtrate, 6~8 parts of aubergine pigment extracting solution, 12~18 parts of chitosan, cornstarch 6~
8 parts.
4. a kind of apple fresh-keeping liquid according to any one of claim 1-3, which is characterized in that the shenqinmycin fermentation
Filtrate is that pseudomonas fluorescens M18 extracts resulting ferment filtrate after shenqinmycin, the filtrate after deep liquid ventilating fermentation
Supernatant is taken after ultrasound, centrifugation, to remove extra fermentation impurities and thallus residue.
5. a kind of apple fresh-keeping liquid according to claim 4, which is characterized in that the pseudomonas fluorescens M18 is by China
Microbiological Culture Collection administration committee common micro-organisms center preservation, deposit number: CGMCC.NO.0462.
6. a kind of apple fresh-keeping liquid according to claim 4, which is characterized in that the frequency of ultrasonic treatment is 800kHZ, when
Between be 30min, the revolving speed of centrifugal treating is 3000rpm, time 5min, and obtained supernatant detects its shenqinmycin through HPLC
Content is in 24.8~32.8mg/L.
7. a kind of apple fresh-keeping liquid according to any one of claim 1-3, which is characterized in that the aubergine pigment extracting solution
It is to extract to obtain by Boiling bath method, controls 1~5 part of aubergine pigment, 80~100 parts of dehydrated alcohol, reacted in boiling water bath
60min is filtered, and supernatant liquor is aubergine pigment extracting solution.
8. the preparation method of apple fresh-keeping liquid, which is characterized in that this method uses following steps:
(1) it dissolves the chitosan in acetum, is configured to chitosan solution；
(2) cornstarch is dissolved in acetum, is heated to 80 DEG C, is configured to corn starch solution；
(3) shenqinmycin ferment filtrate is added after mixing chitosan solution and corn starch solution in equal volume, stirs evenly；
(4) aubergine pigment extracting solution is added in above-mentioned mixed liquor and carries out continuing stirring using same direction, after solution homogeneous,
Up to apple fresh-keeping liquid.
9. the application of apple fresh-keeping liquid, which is characterized in that use following steps:
(1) selected epidermis knocks down fresh-keeping medical fluid without the apple damaged, no disease and pests harm infects, and submerges 5~15min, then picks up
Come, pays attention to guaranteeing that carpopodium will soak；
(2) after having soaked medical fluid, it is packed into ventilation water tank, air-dries the medical fluid of fruit surface；
(3) apple after air-drying is packed into plastic film single fruit freshness protection package, sack is tightened, and 4 DEG C of low-temperature cold store storages are put into.
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Cited By (1)
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| CN101199298A (en) * | 2007-12-19 | 2008-06-18 | 大连工业大学 | Melon and fruit fresh-keeping liquid and preparation method thereof |
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Application publication date: 20181211 |