CN1089651A - The auto thermal compensation method of enzyme electrodes - Google Patents
The auto thermal compensation method of enzyme electrodes Download PDFInfo
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- CN1089651A CN1089651A CN 93100445 CN93100445A CN1089651A CN 1089651 A CN1089651 A CN 1089651A CN 93100445 CN93100445 CN 93100445 CN 93100445 A CN93100445 A CN 93100445A CN 1089651 A CN1089651 A CN 1089651A
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Abstract
The invention relates to biosensor, the particularly invention of the auto thermal compensation of enzyme electrodes, the this kind of enzyme electrode does not need temperature-control device, in cell or close on a thermal sensing element is housed, the working temperature of its enzyme electrodes of perception in good time, in the working process of enzyme electrodes, gather the temperature signal that thermal sensing element provides through temperature measuring circuit by one-chip computer, and carry out auto thermal compensation by the response value of set enzyme electrodes response activity-temperature relation formula knot enzyme electrodes by the one-chip computer program, this method can reduce the complete machine cost of enzyme electrodes instrument, the interference that the bubble of also having avoided the temperature difference to bring is measured enzyme electrodes, the signal to noise ratio of raising enzyme electrodes.
Description
The invention relates to biosensor, the particularly invention of the auto thermal compensation method of enzyme electrodes, this method compensates automatically in conjunction with the response of adopting Temperature Detector and one-chip computer program to enzyme electrodes.
Enzyme electrodes is made up of enzyme membrane and basic electrode (electrochemical sensor).Analyte concentration represents through the transformation of enzyme membrane katalysis and the electrode form with electrical signal.Enzyme membrane is to the catalyzed reaction of substrate and the equal temperature influence of electrode process of basic electrode, considerable temperature factor is arranged respectively, known enzyme electrodes instrument adopts temperature-control device to provide relative isoperibol for enzymatic reaction, but often not high enough because of temperature-controlled precision, often basic electrode is carried out temperature compensation by an extra temperature sensors of high precision and circuit thereof.This method generally can make the enzyme electrodes response free from temperature influence, but cost is higher, when envrionment temperature is lower than controlled temperature (as being lower than 25 ℃) simultaneously when room temperature, current-carrying solution can overflow bubble in cell, increase dry sound source, the interferases determination of electrode, the temperature difference is big more, bubble disturbs serious more, must the designing and arranging bubble assembly.In addition, when envrionment temperature is higher than controlled temperature (as hot season), temperature regulating device reality is inoperative again.
Task of the present invention provides a kind of method, and enzyme electrodes is worked under non-temperature control condition, and in a continuous use, when envrionment temperature changed, the response of whole enzyme electrodes (and being not only basic electrode) was compensated automatically.Owing to do not adopt temperature control unit, both reduced the complete machine cost, the interference that the bubble of also having avoided the temperature difference to bring is measured enzyme electrodes, the dry ratio of letter of raising enzyme electrodes.
The scheme of its solution is: the temperature factor of enzyme electrodes is defined as: 1 ℃ of the every variation of temperature, the multiple of enzyme electrodes response activity change, represent with Q, investigate at first by experiment and the temperature factor of definite enzyme electrodes, set up temperature responds active R to enzyme electrodes functional expression, and deposit one-chip computer in, in the enzyme electrodes cell or its contiguous thermal sensing element of adorning, this thermal sensing element in time sends the working temperature of enzyme electrodes to one-chip computer, is pressed aloud by Single Chip Microcomputer (SCM) program and answers activity-temperature relation formula that the response value of enzyme electrodes is compensated.
Hereinafter reach accompanying drawing and described one embodiment of the present of invention:
Make the enzyme electrodes of measuring glucose with oxygen electrode (CY-2) and glucose oxidase (GOD) enzyme membrane, experiment records, 8～40 ℃ of scopes, enzyme electrodes is to the rising of the response activity with temperature of glucose and increase, temperature factor Q is not a constant, but the function of temperature T, its relational expression is:
Q＝a+bT （1）
If it is To that enzyme electrodes begins the temperature of timing signal, the temperature in arbitrary moment is Ti in the process of a non-stop run, then has
△T＝Ti-To （2）
The temp. compensation type of enzyme electrodes is:
R＝Ri-△T Qoi Ri （3）
In the formula, the enzyme electrodes response value after the R-compensation
The response value of enzyme electrodes when the Ri-temperature is Ti
The Qoi-temperature is the temperature factor of (Ti+To) 1/2 o'clock enzyme electrodes
With (1) and (2) substitution (3) and arrangement,
R＝Ri｛1-（Ti-To）[a+b（Ti+To）1/2]｝ （4）
Consider this embodiment with oxygen electrode as basic electrode, enzyme electrodes is not output as a noble potential through amplifier when not containing glucose in the solution, this output is also drifted about with the drift of temperature, and drift can cause the response slope of enzyme electrodes to change.Therefore, the final temp. compensation type of this enzyme electrodes that draws of combined type (4) is:
R＝Ri｛1-（Ti-To）[a+b（Ti+To）1/2]｝Do/Di （5）
In the formula, enzyme electrodes was to the output signal of blank solution when the Do-temperature was To
Enzyme electrodes was to the output signal of blank solution when the Di-temperature was Ti
If with the hydrogen peroxide electrode is basic electrode, formula (4) can be directly as the temp. compensation type of enzyme electrodes.
Fig. 1 illustrates the structure of this temperature compensating type GOD enzyme electrodes.
Fig. 2 is the temperature sensing circuit schematic diagram.
Fig. 3 is an one-chip computer temperature compensation sub-routine block diagram.
In Fig. 1,2. 1. enzyme electrode gone up at the lucite pedestal through screw threads for fastening, 4. enzyme electrode end (enzyme membrane) 3. seals with fluid course and forms cell 5., 5. one miniature thermal resistor is housed 6. in the bottom at cell, and enzyme electrode and thermistor are connected with separately testing circuit.
In Fig. 2, miniature thermal resistor RT and other three precision resistance type resistor R3, R9 and R10 form bridge circuit, finish the conversion of temperature～voltage by it. Electric bridge adopts the electric current power supply mode, and forms constant-current source by triode BG1 and the BG2 of two characteristic symmetries. After the ratio of each arm of electric bridge was determined, the temperature of bridge circuit～voltage transitions slope was just mainly determined by the size of constant-current source output current, and change W1 potentiometer can be regulated the output current value of constant-current source. Consider the discreteness of thermistor RT parameter, electric bridge carries out calibration by the W2 potentiometer zero point in the time of 0 ℃. In order to guarantee Do not introduce the signal strength signal intensity requirement that bigger paying adds error and satisfy rear level to the measuring bridge circuit, adopted the homophase series winding differential amplifier circuit that is formed by IC1 and IC2. The sampling control signal that triode BG3 acceptance is sent here by computer is finished the relay R Y operation temperature signal of Temperature Detector output is sampled. The temperature detection scope of this Temperature Detector is 0～40 ℃, and nonlinearity is not more than 2%, sensitivity 5mv/ ℃.
In Fig. 3, after the subroutine call, to the zero clearing of thermometric sign, read A/D earlier, determine whether again the thermometric state, because of thermometric sign zero clearing, treat responsive state so continue to determine whether enzyme electrode, if not, deposit data D then0, return. If, judge then whether enzyme electrode response begins, if not, deposit data D then1, return. If then put the thermometric sign, and the temperature control signal is adopted in output. Return and read A/D, enter the thermometric state, obtain the temperature measurement circuit output signal data. Then judge whether enzyme electrode is the demarcation state, if then deposit temperature value T0, if not, enzyme electrode is the mensuration state, deposits temperature value T1 So far thermometric finishes, and returns main program, and by formula (5) executing data is processed.
Claims (1)
- Biosensor, the auto thermal compensation of enzyme electrodes particularly, it is characterized in that: no temperature-control device, in cell or its close on a thermal sensing element be housed, in the working process of enzyme electrodes, gather the temperature signal that thermal sensing element provides through temperature sensing circuit by one-chip computer in good time, and by formula (4) or (5) response value of enzyme electrodes is carried out auto thermal compensation by the one-chip computer program.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 93100445 CN1089651A (en) | 1993-01-12 | 1993-01-12 | The auto thermal compensation method of enzyme electrodes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 93100445 CN1089651A (en) | 1993-01-12 | 1993-01-12 | The auto thermal compensation method of enzyme electrodes |
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| Publication Number | Publication Date |
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| CN1089651A true CN1089651A (en) | 1994-07-20 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 93100445 Pending CN1089651A (en) | 1993-01-12 | 1993-01-12 | The auto thermal compensation method of enzyme electrodes |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8000918B2 (en) | 2007-10-23 | 2011-08-16 | Edwards Lifesciences Corporation | Monitoring and compensating for temperature-related error in an electrochemical sensor |
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1993
- 1993-01-12 CN CN 93100445 patent/CN1089651A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8000918B2 (en) | 2007-10-23 | 2011-08-16 | Edwards Lifesciences Corporation | Monitoring and compensating for temperature-related error in an electrochemical sensor |
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| C01 | Deemed withdrawal of patent application (patent law 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |