CN108949662A - A kind of Escherichia coli fermentation culture medium for gymnoplasm grain pSFVK1-NMM production - Google Patents
A kind of Escherichia coli fermentation culture medium for gymnoplasm grain pSFVK1-NMM production Download PDFInfo
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- 230000004151 fermentation Effects 0.000 title claims abstract description 106
- 239000001963 growth medium Substances 0.000 title claims abstract description 82
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- 238000004519 manufacturing process Methods 0.000 title claims abstract description 21
- 241000894006 Bacteria Species 0.000 claims abstract description 110
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 87
- 239000013612 plasmid Substances 0.000 claims abstract description 53
- 239000010931 gold Substances 0.000 claims abstract description 23
- 229910052737 gold Inorganic materials 0.000 claims abstract description 23
- 229940041514 candida albicans extract Drugs 0.000 claims abstract description 19
- 239000012138 yeast extract Substances 0.000 claims abstract description 19
- 239000012137 tryptone Substances 0.000 claims abstract description 18
- 239000007836 KH2PO4 Substances 0.000 claims abstract description 16
- 239000007640 basal medium Substances 0.000 claims abstract description 16
- 229910000397 disodium phosphate Inorganic materials 0.000 claims abstract description 16
- CSNNHWWHGAXBCP-UHFFFAOYSA-L magnesium sulphate Substances [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims abstract description 16
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 16
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- 238000009630 liquid culture Methods 0.000 claims abstract description 15
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- 239000004471 Glycine Substances 0.000 claims abstract description 5
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- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
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- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4748—Tumour specific antigens; Tumour rejection antigen precursors [TRAP], e.g. MAGE
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
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Abstract
The invention discloses a kind of Escherichia coli fermentation culture mediums for gymnoplasm grain pSFVK1-NMM production.Fermentation medium includes: seed liquid culture medium, fermentation basal medium, fermentation feed medium and micronutrient liquid.Every 1 liter of seed liquid culture medium contains: tryptone 5-7g, yeast extract 10-14g, glycerol 4-6g, Na2HPO4 10‑11g、KH2PO42-3g；Every 1 liter of fermentation basal medium contains: tryptone 10-14g, yeast extract 22-26g, glycerol 8-12g, Na2HPO4 5‑6g、KH2PO4 1‑1.5g、MgSO4Solution: 1-3ml；Every 1 liter of fermentation feed medium contains: glucose 160-200g；Every 100mL micronutrient liquid contains: MgSO42.5g, Nacl 10g, L-Glutamine 5g, glycine 2g, NaH2PO45g.Utilizing works bacterium XL10-Gold/pNMM and the culture medium can obtain plasmid high specific yield, realize the fermenting and producing of NMM tumour fused antigen gymnoplasm grain pSFVK1-NMM by controlling fermentation culture conditions.
Description
Technical field
The invention belongs to gene engineering technology fields, and in particular to one kind is used for NMM tumour fused antigen gymnoplasm grain
The Escherichia coli fermentation culture medium and cultural method of pSFVK1-NMM production.
Background technique
Plasmid is a kind of double-stranded cyclic DNA molecule, carries the hereditary information other than chromosome.Since plasmid is multiple with self
Function processed, a cell contain the plasmid of multiple copies.In past many decades, it is raw that plasmid has become science of heredity, molecule
The most important tool in the fields such as object, especially in recent years, more must not as field of medicaments such as gene therapy and gene vaccines
The tool that can lack.Plasmid can be used for treating single-gene defect class disease, such as bleb cystic fibrosis, muscular dystrophy, hemophilia
Deng, it can also be used to induction is for the infectious diseases such as the hepatitis even specific immune response of tumour.
Plasmid DNA vaccines are a kind of new generation vaccines to begin one's study from the nineties in last century, have been developed till now nearly
30 years, has the characteristics that high security, carrier-free immunogenicity and is readily produced, compared with the vaccine based on virus,
With unique advantage.So far there are 4 kinds of DNA vaccination for animals list marketings, there are also 45 kinds of preventions or the treatment mankind
The DNA vaccination of disease has respectively enterd I, II and III clinical trial phase.With the development of DNA vaccination and gene therapy, for matter
The demand of grain DNA will will increase, and be badly in need of developing easy, time saving, high yield Plasmid DNA production technology.
Research, exploitation and the production of Plasmid DNA vaccines relate generally to upstream, midstream and downstream three phases, and upstream includes
Carrier, gene recombination plasmid building, host strain and engineering strain word bank.Middle reaches are mainly bacterial fermentation.The emphasis in downstream is matter
The separation and purifying of grain DNA.NMM tumour fused antigen gymnoplasm grain pSFVK1-NMM molecular weight is larger, reaches 15K or so, for
It is a kind of heavy burden for host strain.In the growth course of host strain, the unstable of Plasmid DNA is easily caused, thus
Influence the copy number of plasmid in single bacterium.The parameter and condition main purpose for optimizing the culture of host's engineering bacterium fermentation are to mention
High yield makes to obtain thallus as much as possible from every liter of culture medium, then extracts plasmid as much as possible from every gram of thallus
DNA.Wanting to obtain higher yield generally will ingredient and special fermentation (dissolved oxygen feedback-type feed supplement hair Jing Guo Optimal Medium
Ferment) strategy realizes.There is the more research about recombinant protein fermentation condition optimization to report at present, and plasmid DNA fermentation condition
It is different with recombinant protein fermentation condition, it is again considerably less for the design optimization of plasmid DNA fermentation culture medium and condition of culture,
In this case, establishing the scientific and reasonable method that a kind of pair of plasmid fermented and cultured optimizes is very important.
Summary of the invention
In order to overcome the disadvantages and deficiencies of the prior art, the purpose of the present invention is to provide a kind of Escherichia coli fermentation cultures
Base, the production for NMM tumour fused antigen gymnoplasm grain pSFVK1-NMM
The object of the invention, which also resides in, provides the engineering strain word bank of production NMM tumour fused antigen gymnoplasm grain pSFVK1-NMM
Construction method and seed bank product.It is that conversion has for producing the engineering bacteria of NMM tumour fused antigen gymnoplasm grain pSFVK1-NMM
The Escherichia coli XL10-Gold of plasmid pSFVK1-NMM, is named as XL10-Gold/pNMM.
The object of the invention is also to provide the fermentation process in high density based on above-mentioned Escherichia coli fermentation culture medium.The party
Method utilizes above-mentioned engineering bacteria and fermentation medium, and controls fermentation culture conditions, to obtain the engineering of high specific yield
XL10-Gold/pNMM。
A kind of Escherichia coli fermentation culture medium for gymnoplasm grain pSFVK1-NMM production,
Every 1 liter of seed liquid culture medium contains: tryptone 5-7g, yeast extract 10-14g, glycerol 4-6g, Na2HPO4
10-11g、KH2PO42-3g；
Every 1 liter of fermentation basal medium contains: tryptone 10-14g, yeast extract 22-26g, glycerol 8-12g,
Na2HPO4 5-6g、KH2PO4 1-1.5g、MgSO4Solution: 1-3ml；
Every 1 liter of fermentation feed medium contains: glucose 160-200g；
Every 100mL micronutrient liquid contains: MgSO42.5g, Nacl 10g, L-Glutamine 5g, glycine 2g,
NaH2PO4 5g。
The Escherichia coli fermentation culture medium the preparation method is as follows:
Every 1 liter of seed liquid culture medium the preparation method comprises the following steps: weigh tryptone 5-7g, yeast extract 10-14g, glycerol
4-6g、Na2HPO4 10-11g、KH2PO4Deionized water dissolving, constant volume to 1000mL, 121 DEG C of high pressure sterilizations are added in 2-3g
It is saved backup for 4 DEG C after 20min；
Fermentation every 1 liter of basal medium the preparation method comprises the following steps: weigh tryptone 10-14g, yeast extract 22-26g,
Glycerol 8-12g, Na2HPO4 10-11g、KH2PO42-3g, is added deionized water dissolving, constant volume to 1000mL, and 121 DEG C of high pressures are gone out
It is saved backup for 4 DEG C after bacterium 30min；Before fermented and cultured starts, MgSO is added in every 1 liter of fermentation basal medium4Solution 1-3ml；
MgSO4Solution manufacturing method are as follows: weigh MgSO4Solid 50g, is added deionized water dissolving, constant volume to 100mL, and 0.22um filter membrane removes
It is saved backup for 4 DEG C after bacterium filtering；
Every 1 liter of fermentation feed medium the preparation method comprises the following steps: weigh glucose 180g, be added deionized water dissolving, constant volume
To 1000mL, saved backup for 4 DEG C after 115 DEG C of high pressure sterilization 30min；
The configuration method of the every 100mL of micronutrient liquid are as follows: weigh MgSO4It is 2.5g, Nacl 10g, L-Glutamine 5g, sweet
Propylhomoserin 2g, NaH2PO4Deionized water dissolving is added in 5g, and constant volume to 100mL, 4 DEG C of preservations are standby after 0.22um filter membrane aseptic filtration
With.
The construction method of XL10-Gold/pNMM engineering strain word bank carries out in accordance with the following steps:
(1) it prepares original species word bank: the engineering bacteria XL10-Gold/pNMM is activated to the LB solid containing kanamycins
30 DEG C of cultures, picking monoclonal are inoculated into the test tube of the culture medium of LB containing 5mL and are incubated overnight 12h for 30 DEG C, inoculate in plate
12h is incubated overnight for 30 DEG C in the centrifuge tube of the LB containing 20mL, and glycerol is added to final concentration 20%, dispenses 10 pipes and freezes in -80 DEG C of ice
It is saved in case, obtains original species word bank；
(2) prepare main seed bank: one primordial seed of recovery is inoculated in 8 pipe LB liquid mediums, and matter is extracted after passage
Grain is chosen the highest bacterium solution of plasmid specificity yield and is expanded in culture to the culture medium of LB containing 100mL, trains under 30 DEG C, 200RPM
Glycerol is added to final concentration 20% after supporting 12h, 50 pipes of packing freeze to be saved in -80 DEG C of refrigerators, obtains main seed bank；
(3) preparation work seed bank: one main seed of recovery is inoculated in 8 pipe LB liquid mediums, and matter is extracted after passage
Grain is chosen the highest bacterium solution of plasmid specificity yield and is expanded in culture to the culture medium of LB containing 100mL, trains under 30 DEG C, 200RPM
Glycerol is added to final concentration 20% after supporting 12h, 50 pipes of packing freeze to be saved in -80 DEG C of refrigerators, obtains work seed bank.Every
Strain limit in work seed bank passes the five alternative productions in NMM tumour fused antigen gymnoplasm grain pSFVK1-NMM.
A kind of Escherichia coli fermentation cultural method for NMM tumour fused antigen gymnoplasm grain pSFVK1-NMM production, packet
Containing following steps:
(1) prepared by seed liquor: it works seeds in -80 DEG C of refrigerator recovery engineering bacteria XL10-Gold/pNMM, with 0.6%~
1% ratio is inoculated in seed liquid culture medium, by the triangular flask equipped with seed liquor with 30 DEG C in constant-temperature table, 180-220r/
Min cultivates 12-14 hours, if bioreactor volume is larger, can increase the preparation of second level and three-level seed liquor；
(2) fermented and cultured: being inoculated in bioreactor for seed liquor with 8%~10% ratio, opens under equipment control
Beginning fermented and cultured, fermentor are provided with fermentation basal medium；In fermentation process by bioreactor fill into automatically hydrochloric acid and
Ammonium hydroxide makes pH value control 7 ± 0.1；Fermented and cultured 0-8h cultivation temperature is 30 DEG C, and fermented and cultured 8-12h cultivation temperature is 37
℃；And 20mL/L micronutrient liquid is added after fermented and cultured 8 hours；By improving ventilatory capacity in revolving speed and tank, control molten
Oxygen is 30% or more, until revolving speed reaches 600RPM, ventilation reaches 500L/h；With the training of dissolved oxygen feedback-type gradient constant speed flow feeding
Support the mode feed supplement of base；To 12 hours end fermented and cultureds, by obtained bacterium solution to be centrifuged 20 minutes collection bacterium not less than 6000g
Body measures fermentation parameter.
Seed liquor cultivation temperature is 30 degree, and fermentation 0h to cultivation temperature between 8h is 30 DEG C, fermentation 8h to 12h cultivation temperature
It is 37 DEG C.
The dissolved oxygen feedback-type gradient constant speed flow feeding mode, comprises the following steps:
When oxygen dissolving value because being added automatically when subalimentation obviously rises for the first time in fermentor with the flow velocity of 10mL/min/L
Supplemented medium；
When oxygen dissolving value because being mended automatically in fermentor when second of obvious rising of subalimentation with the flow velocity of 15mL/min/L
Add supplemented medium；
When oxygen dissolving value because being mended automatically when subalimentation third time obviously rises in fermentor with the flow velocity of 20mL/min/L
Add supplemented medium；
When oxygen dissolving value because being mended automatically in fermentor when the 4th obvious rising of subalimentation with the flow velocity of 25mL/min/L
Add supplemented medium.
Beneficial effects of the present invention: the work of production NMM tumour fused antigen gymnoplasm grain pSFVK1-NMM provided by the invention
Journey bacterium seed bank construction method is that conversion has matter for producing the engineering bacteria of NMM tumour fused antigen gymnoplasm grain pSFVK1-NMM
The Escherichia coli XL10-Gold of grain pSFVK1-NMM, is named as XL10-Gold/pNMM.Escherichia coli fermentation culture of the invention
Base, the production for NMM tumour fused antigen gymnoplasm grain pSFVK1-NMM.The present invention uses the height of Escherichia coli fermentation culture medium
Density fermentation process, this method utilizes above-mentioned engineering bacteria and fermentation medium, and controls fermentation culture conditions, to obtain Gao Te
The engineering bacteria XL10-Gold/pNMM of anisotropic yield.
Detailed description of the invention
Fig. 1 is the Escherichia coli effect picture that 37 degree of cultures carry pSFVK1-NMM plasmid；In figure, 1,15K DNA
Marker, 37 degree of 2, engineering bacteria culture first generation, 3, the liang of generation of 37 degree of engineering bacteria cultures, 37 degree of 4, engineering bacteria culture third generations.
Fig. 2 is that the Escherichia coli of 37 degree of culture carrying pSFVK1-NMM plasmids are more than 10 hours effect pictures；In figure, 1,15K
DNA Marker, 2,37 degree of engineering bacteria cultivate 6 hours, 3,37 degree of engineering bacteria cultivate 8 hours, 4,37 degree of engineering bacteria to cultivate 10 small
When, 5,37 degree of engineering bacteria cultivate 12 hours.
Fig. 3 is the Escherichia coli effect picture that 30 degree of cultures carry pSFVK1-NMM plasmid；In figure, 1,15K DNA
Marker, 30 degree of 2, engineering bacteria culture 1st generations, 3,30 degree of engineering bacteria the 10th generations of culture.
Fig. 4 is the growth curve of the engineering bacteria of fermented and cultured under four kinds of temperature match curing conditions.
Fig. 5 is the growth curve of the engineering bacteria of fermented and cultured under different supplemented mediums.
Fig. 6 is the growth curve of the engineering bacteria under 0-14 hours fermented and cultureds.
Fig. 7 is the growth curve of the engineering bacteria of continuous three batches of fermented and cultureds under pilot-scale.
Specific embodiment
The present invention will be further described in the following with reference to the drawings and specific embodiments.
The screening of 1 seed liquor cultivation temperature of embodiment
Since the molecular weight of NMM tumour fused antigen gymnoplasm grain pSFVK1-NMM is larger, reach 15K or so, for host
It is a kind of heavy burden for bacterium.In the growth fission process of engineering bacteria XL10-Gold/pNMM, Plasmid DNA is easily caused
It is unstable.It is found by early-stage study, if cultivation temperature is excessively high, it will cause Plasmid DNA size variations；Temperature is too low,
Then bacterial growth is too slow, and seed liquor needs to cultivate for a long time.
Firstly, test, under conditions of 37 degree, 200 revs/min, LB culture medium, 12 hours are a generation, continuous culturing engineering bacterium
Three generations, then extracts plasmid with the small extraction reagent kit of plasmid, and agarose gel electrophoresis shows that plasmid pSFVK1-NMM can be different degrees of
Diminution.So engineering bacteria XL10-Gold/pNMM cannot be cultivated at 37 degree for a long time.
Then it tests under conditions of 37 degree, 200 revs/min, LB culture medium, divides after culturing engineering bacterium 6,8,10,12 hours
It not sampling, extracts plasmid with the small extraction reagent kit of plasmid, agarose gel electrophoresis is shown, when 37 degree of cultures are small more than 10, plasmid
PSFVK1-NMM will be different degrees of diminution.It is small 10 to illustrate that engineering bacteria XL10-Gold/pNMM needs to control in 37 degree of cultures
When within.
Last test at 30 degree, 200 revs/min, under the conditions of, 12 hours be a generation, in continuous 10 generation of culturing engineering bacterium, then use
The small extraction reagent kit of plasmid extracts plasmid, and agarose gel electrophoresis shows plasmid pSFVK1-NMM size and is expected unanimously, to illustrate work
Journey bacterium can be in 30 degree of cultures steady in a long-term.
The screening of embodiment 2, seed liquid culture medium HGSM (HG seed medium)
The purpose of seed liquor culture is to obtain in the short time that Bacteria cold shock is good and the enough bacterium solutions of concentration, after being
Continuous fermentation tank culture is prepared.To filter out most suitable seed liquid culture medium HGSM, by engineering bacteria E.coli XL10-
Gold/pNMM is inoculated in following culture medium respectively, compares cultivation results.
LB culture medium --- every 1 liter of LB culture medium contains: tryptone 10g, yeast extract 5g, Nacl 10g.It is added
Deionized water dissolving is settled to 1000mL, saves backup for 4 DEG C after 121 DEG C of high pressure sterilization 20min.
LBC culture medium --- every 1 liter of LBC culture medium contains: tryptone 10g, yeast extract 5g, Nacl10g, grape
Sugared 5g.Deionized water dissolving is added, is settled to 1000mL, is saved backup for 4 DEG C after 121 DEG C of high pressure sterilization 20min.
TB culture medium --- every 1 liter of TB culture medium contains: tryptone 12g, yeast extract 24g, Na2HPO4
10.22g、KH2PO42.32g, glycerol 5g.Deionized water dissolving is added, is settled to 1000mL, 4 after 21 DEG C of high pressure sterilization 20min
It DEG C saves backup.
TB1 culture medium --- every 1 liter of TB culture medium contains: tryptone 18g, yeast extract 18g, Na2HPO4
10.22g、KH2PO42.32g, glycerol 5g.Deionized water dissolving is added, is settled to 1000mL, 4 after 121 DEG C of high pressure sterilization 20min
It DEG C saves backup.
TBC culture medium --- every 1 liter of TBC culture medium contains: tryptone 6g, yeast extract 12g, Na2HPO4
10.22g、KH2PO42.32g, glycerol 10g.Deionized water dissolving is added, is settled to 1000mL, after 121 DEG C of high pressure sterilization 20min
4 DEG C save backup.
The every 1 liter of TBC1 culture medium of TBC1 culture medium-contains: tryptone 9g, yeast extract 9g, Na2HPO4
10.22g、KH2PO42.32g, glycerol 10g.Deionized water dissolving is added, is settled to 1000mL, after 121 DEG C of high pressure sterilization 20min
4 DEG C save backup.
In order to facilitate comparative evaluation, Uniform provisions shake flask culture conditions are as follows: one XL10-Gold/pNMM work kind of recovery
Son is inoculated in the culture medium of 240mL by 1:200, and sampling and measuring OD600 after 30 DEG C, 200RPM culture 12 hours claims after centrifugation
Measure wet bacterium.By comparing, bacterium solution OD600 value and the highest one group of culture medium of bacterium yield are selected as seed liquid culture medium.
Table 1 is bacterium yield and bacterium solution OD600 value under the conditions of different culture medium.
| Culture medium classification | OD600 |
| LB culture medium | 1.597 |
| LB-A culture medium | 1.676 |
| TB culture medium | 1.513 |
| TB-A culture medium | 1.402 |
| TBC culture medium | 1.967 |
| TBC-A culture medium | 1.483 |
Can be obtained by above table data: under same culture conditions, engineering bacteria XL10-Gold/pNMM is cultivated in TBC
The speed of growth is most fast in base, bacterium solution OD600 value highest.So selecting TBC culture medium as seed liquid culture medium HGSM.
Embodiment 3, ferment basal medium HGBM (HG basic medium) screening
It before carrying out fermentation tank culture, needs to be cultivated in shaking table scale first, finds out most suitable fermentation training
Base is supported, is laid the foundation for follow-up study.This part is using shaking table culture as main means, and Primary Study engineering bacteria is in different trainings
The case where supporting bacterium yield and specific yield in base, the best medium found out will be as " fermentation basal medium HGBM (HG
Basic medium) ", in order to facilitate comparative evaluation, the condition of our Uniform provisions shaking flask culture is as follows:
Work seed inoculative proportion: 1:100
Culture volume: 250mL culture medium/1000mL conical flask
Shaking table parameter: 30 DEG C, 200RPM
Incubation time: 12h
Culture for engineering bacteria, we pay close attention to following two core parameter:
1) in bacterium yield (g/L): every liter culture solution, the wet bacterium amount that can harvest.
2) specific yield (mg/g): in every gram of wet bacterium, the quality of contained Plasmid DNA.
When comparing different fermentations tank condition of culture, it can be analyzed and evaluated by comparing two above parameter.It is excellent
First select specific yield higher, followed by bacterium yield.
(1) the conventional medium LB and TB for comparing Escherichia coli first compare bacterium yield and specific yield, such as 2 institute of table
Show:
Table 2
TB culture medium either bacterium yield or bacterium specificity yield, better than LB culture medium, therefore select to train in TB
Continue to screen on the basis of feeding base.
(2) on the basis of TB culture medium, the ratio of peptone and yeast extract is changed, bacterium yield and specificity are compared
Yield, as shown in table 3:
Table 3
Wherein, although TB1 culture medium bacterium yield is minimum, bacterium specificity yield is best.We select to cultivate in TB1
Continue to screen on the basis of base.
(3) on the basis of TB1 culture medium, the various concentration of glycerol is adjusted, compares bacterium yield and specific yield, such as table
Shown in 4:
Table 4
Wherein, the bacterium specificity yield highest of TB8 culture medium.We select to continue on the basis of TB8 culture medium excellent
Change.
(4) on the basis of TB8 culture medium, adjustment phosphate concn and increase MgSO4, compare bacterium yield and specificity produce
Rate, as shown in table 5:
Table 5
The bacterium specificity yield highest of TB10 culture medium, therefore using the TB10 culture medium filtered out as " fermentation basis
Culture medium HGBM (HG basic medium) " is used for subsequent fermentation test.
The screening of embodiment 4, fermentation temperature
Since plasmid pSFVK1-NMM is larger, it will appear division unstability in host strain reproduction process, lead to plasmid
PSFVK1-NMM mass is undesirable.Inventor has found that engineering bacteria XL10-Gold/pNMM is trained at 30 DEG C by shake flat experiment
It is slow to support the speed of growth, enough bacterial concentrations can not be reached in a short time, but plasmid stability is preferable；And it is trained at 37 DEG C
Although supporting engineering bacteria fast growing but plasmid stability being poor, is detected and found by agarose gel electrophoresis, 37 DEG C of engineering bacteria trainings
6 hours or more plasmids are supported to start to be gradually reduced.So the present invention uses 30 degree of fermentation initial stage, ferment 37 degree of temperature-variable fermentation of later period
Mode accelerates the engineering bacteria speed of growth under the premise of guaranteeing plasmid pSFVK1-NMM mass as far as possible.Temperature turns in this mode
The time point changed has important influence to entire fermentation results.In order to facilitate comparison, the different alternating temperature times are explored to zymotechnique
Fermentation total duration is set to 12 hours in following comparative testing by the influence (Fig. 4) of parameter, the specific steps are as follows:
The shaking flask culture of seed liquor: work seed being inoculated in seed liquid culture medium with 1:100,30 degree, 200 revs/min,
Culture 13 hours；
Fermentation tank culture: seed liquor is inoculated in 3L fermentation basal medium in 10% ratio, is initially carried out with 30 degree
Culture；Respectively the 6th hour after fermentation starts, 7 hours, 8 hours, 9 hours, temperature is increased to 37 degree from 30 degree；Work as dissolved oxygen
Because when subalimentation starts rapid increase in fermentor, with dissolved oxygen feedback-type gradient constant speed stream glycerol adding (180g/L) solution
Mode feed supplement；Fermentation terminates after 12 hours, and thalline were collected by centrifugation, measurement bacterium yield and specific yield.
Table 6 is that fermentation different temperatures changes lower biomass, the comparison of specific yield
| Number | 30 degree of duration | 37 degree of duration | Bacterium yield (g/L) | Specific yield (mg/g) |
| 1 | 6 hours | 6 hours | 68 | 0.87 |
| 2 | 7 hours | 5 hours | 69.5 | 0.79 |
| 3 | 8 hours | 4 hours | 64.9 | 1.13 |
| 4 | 9 hours | 3 hours | 68.6 | 1.04 |
Temperature transition time point it is different bacterium yield difference it is little, but 30 degree are warming up to 37 degree and continue to train after culture 8 hours
It supports, the engineering bacteria specificity yield highest of this temperature transition time point culture.
The influence of embodiment 5, feed supplement composition to fermented and cultured
After engineering bacteria is cultivated 8 hours with 30 degree of basal fermentation medium in the fermenter, fermentation temperature is improved to 37 degree
Continue to cultivate.At this time since temperature rises, engineering bacteria breeding and faster metabolism, oxygen dissolving value will be gradually decreased.It is fermenting about
After 10 hours, it is found that oxygen dissolving value rises rapidly, and illustrates that the nutriment in fermentor at this time is depleted by engineering bacteria,
It needs that nutriment is replenished in time.The feed supplement in such a way that dissolved oxygen feedback-type gradient constant speed stream adds of this project is specific to mend to fermentation ends
Material mode is as follows:
When oxygen dissolving value because being added automatically when subalimentation obviously rises for the first time in fermentor with the flow velocity of 10mL/min/L
Supplemented medium；
When oxygen dissolving value because being mended automatically in fermentor when second of obvious rising of subalimentation with the flow velocity of 15mL/min/L
Add supplemented medium；
When oxygen dissolving value because being mended automatically when subalimentation third time obviously rises in fermentor with the flow velocity of 20mL/min/L
Add supplemented medium；
When oxygen dissolving value because being mended automatically in fermentor when the 4th obvious rising of subalimentation with the flow velocity of 25mL/min/L
Add supplemented medium；
Different feed supplement compositions has a significant effect (Fig. 5) to the growth of fermentation later period bacterium, and glucose or glycerol is selected to make
For supplemented medium, the difference of every fermentation parameter is compared.In order to facilitate comparison, fermentation total duration is determined in following comparative testing
It is 12 hours, while the concentration of glucose and glycerol is 180g/L.
Table 7
| Number | Feed supplement composition | Feed supplement initial time | Bacterium yield (g/L) | Specific yield (mg/g) |
| 1 | Glycerol | 10.2 hours | 64.9 | 1.13 |
| 2 | Glucose | 10.1 hours | 64 | 1.3 |
As can be seen from Table 7, using glucose (180g/L) or glycerol (180g/L) as supplemented medium, the bacterium of the two
Yield is almost the same, but supplements the engineering bacteria plasmid specificity yield that glucose obtains and be apparently higher than supplement glycerol.
Embodiment 6, the screening for total time of fermenting
Bacterium grows in the fermenter is divided into lag phase, logarithmic growth phase and plateau.The seed liquor of high concentration and high-incidence
Fermentation tank inoculative proportion can shorten lag phase.It is interim in logarithmic growth, the number meeting rapid growth of bacterium, but single bacterium
Plasmid content not necessarily can be very high.General arrival logarithmic growth phase end or plateau, number of bacteria no longer grow at top speed
When, the plasmid content of single bacterium can gradually increase.But it due to being free of antibiotic in fermentation medium, does not select to press
Power, if bacterium is in time plateau too long, the bacterium without plasmid can be more and more, cause the decline of specific yield.
So it is particularly significant to find out the suitable fermentation termination time.
After engineering bacteria is cultivated 8 hours with 30 degree of basal fermentation medium in the fermenter, fermentation temperature is improved to 37 degree
Continue to cultivate.Due to subalimentation in fermentor after fermentation about 10 hours, added by dissolved oxygen feedback-type gradient constant speed stream
Mode feed supplement.Since 37 DEG C of incubation times were no more than 6 hours, so fermentation was terminated to 14 hours.Comparison 10 to 14 hours
Bacterium yield and bacterium specificity yield determine that best ferment fermentation terminates time (Fig. 6, table 8).
Table 8 is fermentation 10h to 14h bacterium yield, specific yield compares
| Fermentation time | OD600 | Bacterium yield (g/L) | Specific yield (mg/g) |
| 10 hours | 18.27 | 47.5 | 0.92 |
| 11 hours | 19.26 | 51.5 | 1.17 |
| 12 hours | 21.22 | 54.7 | 1.44 |
| 13 hours | 22.34 | 58 | 1.32 |
| 14 hours | 23.27 | 62.8 | 1.12 |
Fermentation 0 to 5 hour, number of bacteria increasess slowly；Enter within 5 to 10 hours logarithmic phase, number of bacteria rapid growth；10
After hour, into plateau, number of bacteria slowdown in growth.For bacterium specificity yield, it is small that peak appears in fermentation the 12nd
When；After 12 hours, although bacterium yield is continuing to increase, specific yield is gradually reduced.Possible cause is due to culture
There is no antibiotic in base, when fermenting small more than 12 after, the engineering bacteria number in fermentation liquid containing plasmid is gradually reduced, without matter
The number of bacteria of grain is gradually increasing, and ultimately causes the decline of specific yield.Therefore the most suitable fermentation termination time is 12 small
When.
Embodiment 7, in 5L fermentor scale evaluation engineering bacteria XL10-Gold/pNMM production of plasmid pSFVK1-NMM
By the research to culture medium and training method, optimal 5L fermentation tank culture step is obtained are as follows:
(1) preparation of various culture mediums needed for fermenting:
Prepare 300ml seed liquid culture medium HGSM: weigh tryptone 1.8g, yeast extract 3.6g, glycerol 1.5g,
Na2HPO4 3.07g、KH2PO4Deionized water dissolving, constant volume to 300mL, 4 DEG C after 121 DEG C of high pressure sterilization 20min is added in 0.7g
It saves backup；
Prepare 10ml MgSO4Solution: MgSO is weighed4Deionized water dissolving, constant volume to 10mL, 0.22um is added in solid 5g
It is saved backup for 4 DEG C after filter membrane aseptic filtration；
It prepares 3L fermentation basal medium HGBM: weighing tryptone 36g, yeast extract 72g, glycerol 30g, Na2HPO4
15.33g、KH2PO43.48g, is added deionized water dissolving, and constant volume to 3L saves backup for 4 DEG C after 121 DEG C of high pressure sterilization 30min；
Before fermented and cultured starts, MgSO is added into fermentation basal medium4Solution 6ml；
It prepares 300ml fermentation feed medium HGAM: weighing glucose 54g, deionized water dissolving is added, constant volume arrives
It is saved backup for 4 DEG C after 300mL, 115 DEG C of high pressure sterilization 30min.
It prepares 100ml micronutrient liquid: weighing MgSO41g, Nacl 4g, L-Glutamine 2g, glycine 0.8g,
NaH2PO42g, is added deionized water dissolving, and constant volume to 100mL saves backup for 4 DEG C after 0.22um filter membrane aseptic filtration.
(2) preparation of seed liquor:
300ml kind is inoculated in 8% ratio in two engineering bacteria XL10-Gold/pNMM work seeds of -80 DEG C of refrigerators recoveries
In sub- liquid culture medium.By the triangular flask equipped with seed liquor 30 DEG C, 200r/min in constant-temperature table, cultivate 13 hours.
(3) 5L fermentation tank culture:
Seed liquor is inoculated in the bioreactor of 5L with 10% ratio, starts fermented and cultured under equipment control, sends out
Fermentation basal medium containing 3L in fermentation tank；In fermentation process hydrochloric acid and ammonium hydroxide are filled by bioreactor automatically, makes PH
Value control is 7 ± 0.1；Cultivation temperature is increased to 37 DEG C after being 30 DEG C, fermented and cultured 8 hours by fermented and cultured 0-8h cultivation temperature
20mL/L micronutrient liquid is added simultaneously；By improving ventilatory capacity in revolving speed and tank, dissolved oxygen is controlled 30% or more, until revolving speed
Reach 600RPM, ventilation reaches 10L/min；When oxygen dissolving value because obvious ascendant trend occurs for the first time in subalimentation in fermentor
When, supplemented medium is added automatically with the flow velocity of 10mL/min/L, when dissolved oxygen occur second, third time, the 4th time it is obvious on
When the trend of liter, corresponding Feed flow rate is 15mL/min/L, 20mL/min/L, 25mL/min/L.It is sent out to end in 12 hours
Obtained bacterium solution is centrifuged 20 minutes collection thallus with 8000g, measures fermentation parameter by ferment culture.
According to the above fermentation step, 3 fermentations have been carried out in 5L fermentor (3L culture solution is housed) scale.Fig. 7 is pilot scale
The growth curve of the engineering bacteria of continuous three batches of fermented and cultureds under scale.
In order to confirm the above culture medium and cultural method better than Bacillus coli communis training method, use TB culture medium as pair
According to culture medium, by the conventional training method that once feeds intake under the conditions of 30 degree, cultivate into fermentor until nutrient exhaustion,
Other condition of culture are identical as experimental group.Specific fermentation parameter such as table 9:
Table 9
| Number | OD600 | Bacterium yield (g/L) | Specific yield (mg/g) |
| Experimental group 1 | 24.49 | 64.7 | 1.16 |
| Experimental group 2 | 24.26 | 63.7 | 1.4 |
| Experimental group 3 | 24.96 | 61 | 1.26 |
| Control group | 15.1 | 38.1 | 0.71 |
The culture medium and specific process fermentation XL10-Gold/pNMM obtained with present invention research, production of plasmid
PSFVK1-NMM, plasmid specificity yield can be stablized in 1.15mg/g or more, and bacterium yield can also reach 60-65g/L.And control group
Plasmid specificity yield is 0.71mg/g, and bacterium yield also only has 38.1g/L, it is seen that the culture medium and cultural method that the present invention studies
Better than Bacillus coli communis fermentation method.
Claims (6)
1. a kind of Escherichia coli fermentation culture medium for gymnoplasm grain pSFVK1-NMM production, it is characterised in that:
Every 1 liter of seed liquid culture medium contains: tryptone 5-7g, yeast extract 10-14g, glycerol 4-6g, Na2HPO410-
11g、KH2PO42-3g；
Every 1 liter of fermentation basal medium contains: tryptone 10-14g, yeast extract 22-26g, glycerol 8-12g,
Na2HPO45-6g、KH2PO4 1-1.5g、MgSO4Solution: 1-3ml；
Every 1 liter of fermentation feed medium contains: glucose 160-200g；
Every 100mL micronutrient liquid contains: MgSO42.5g, Nacl 10g, L-Glutamine 5g, glycine 2g, NaH2PO45g。
2. the Escherichia coli fermentation culture medium for gymnoplasm grain pSFVK1-NMM production, feature exist according to claim 1
In, the Escherichia coli fermentation culture medium the preparation method is as follows:
Every 1 liter of seed liquid culture medium the preparation method comprises the following steps: weigh tryptone 5-7g, yeast extract 10-14g, glycerol 4-6g,
Na2HPO4 10-11g、KH2PO4Deionized water dissolving, constant volume to 1000mL, 4 after 121 DEG C of high pressure sterilization 20min is added in 2-3g
It DEG C saves backup；
Fermentation every 1 liter of basal medium the preparation method comprises the following steps: weigh tryptone 10-14g, yeast extract 22-26g, glycerol
8-12g、Na2HPO4 10-11g、KH2PO4Deionized water dissolving, constant volume to 1000mL, 121 DEG C of high pressure sterilizations are added in 2-3g
It is saved backup for 4 DEG C after 30min；Before fermented and cultured starts, MgSO is added in every 1 liter of fermentation basal medium4Solution 1-3ml；
MgSO4Solution manufacturing method are as follows: weigh MgSO4Deionized water dissolving, constant volume to 100mL, 0.22um filter is added in solid 50g
It is saved backup for 4 DEG C after film aseptic filtration；
Every 1 liter of fermentation feed medium the preparation method comprises the following steps: weigh glucose 180g, deionized water dissolving is added, constant volume arrives
It is saved backup for 4 DEG C after 1000mL, 115 DEG C of high pressure sterilization 30min；
The configuration method of the every 100mL of micronutrient liquid are as follows: weigh MgSO42.5g, Nacl 10g, L-Glutamine 5g, glycine
2g、NaH2PO45g, is added deionized water dissolving, and constant volume to 100mL saves backup for 4 DEG C after 0.22um filter membrane aseptic filtration.
The construction method of 3.XL10-Gold/pNMM engineering strain word bank, which is characterized in that carry out in accordance with the following steps:
(1) it prepares original species word bank: the engineering bacteria XL10-Gold/pNMM is activated to the LB solid plate containing kanamycins
In 30 DEG C of cultures, picking monoclonal is inoculated into the test tube of the culture medium of LB containing 5mL and is incubated overnight 12h for 30 DEG C, inoculate and contain
12h is incubated overnight for 30 DEG C in the centrifuge tube of 20mL LB, and glycerol is added to final concentration 20%, dispenses 10 pipes and freezes in -80 DEG C of refrigerators
Middle preservation obtains original species word bank；
(2) prepare main seed bank: one primordial seed of recovery is inoculated in 8 pipe LB liquid mediums, and plasmid is extracted after passage, is selected
It takes the highest bacterium solution of plasmid specificity yield to expand in culture to the culture medium of LB containing 100mL, cultivates 12h under 30 DEG C, 200RPM
Glycerol is added afterwards to final concentration 20%, 50 pipes of packing freeze to be saved in -80 DEG C of refrigerators, obtains main seed bank；
(3) preparation work seed bank: one main seed of recovery is inoculated in 8 pipe LB liquid mediums, and plasmid is extracted after passage, is selected
It takes the highest bacterium solution of plasmid specificity yield to expand in culture to the culture medium of LB containing 100mL, cultivates 12h under 30 DEG C, 200RPM
Glycerol is added afterwards to final concentration 20%, 50 pipes of packing freeze to be saved in -80 DEG C of refrigerators, obtains work seed bank.Every work
Strain limit in seed bank passes the five alternative productions in NMM tumour fused antigen gymnoplasm grain pSFVK1-NMM.
4. a kind of Escherichia coli fermentation cultural method for NMM tumour fused antigen gymnoplasm grain pSFVK1-NMM production, special
Sign is, comprises the following steps:
(1) prepared by seed liquor: in -80 DEG C of refrigerator recovery engineering bacteria XL10-Gold/pNMM work seeds, with 0.6%~1% ratio
Example is inoculated in seed liquid culture medium, by the triangular flask equipped with seed liquor with 30 DEG C, 180-220r/min in constant-temperature table, training
It supports 12-14 hours, if bioreactor volume is larger, the preparation of second level and three-level seed liquor can be increased；
(2) fermented and cultured: being inoculated in bioreactor for seed liquor with 8%~10% ratio, starts to send out under equipment control
Ferment culture, fermentor are provided with fermentation basal medium；Hydrochloric acid and ammonium hydroxide are filled by bioreactor automatically in fermentation process,
Make pH value control 7 ± 0.1；Fermented and cultured 0-8h cultivation temperature is 30 DEG C, and fermented and cultured 8-12h cultivation temperature is 37 DEG C；And
And 20mL/L micronutrient liquid is added after fermented and cultured 8 hours；By improving ventilatory capacity in revolving speed and tank, control dissolved oxygen exists
30% or more, until revolving speed reaches 600RPM, ventilation reaches 500L/h；With dissolved oxygen feedback-type gradient constant speed flow feeding culture medium
Mode feed supplement；To 12 hours end fermented and cultureds, obtained bacterium solution is centrifuged 20 minutes collection thallus to be not less than 6000g,
Measure fermentation parameter.
5. fermentation culture method according to claim 4, it is characterized in that, seed liquor cultivation temperature is 30 degree, and ferment 0h
It is 30 DEG C to cultivation temperature between 8h, fermentation 8h to 12h cultivation temperature is 37 DEG C.
6. fermentation culture method according to claim 4, it is characterized in that, the dissolved oxygen feedback-type gradient constant speed stream adds benefit
Material mode, comprises the following steps:
When oxygen dissolving value because adding feed supplement automatically when subalimentation obviously rises for the first time in fermentor with the flow velocity of 10mL/min/L
Culture medium；
When oxygen dissolving value because being added automatically in fermentor when second of obvious rising of subalimentation with the flow velocity of 15mL/min/L
Expect culture medium；
When oxygen dissolving value because being added automatically when subalimentation third time obviously rises in fermentor with the flow velocity of 20mL/min/L
Expect culture medium；
When oxygen dissolving value because being added automatically in fermentor when the 4th obvious rising of subalimentation with the flow velocity of 25mL/min/L
Expect culture medium.
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