CN108949560B

CN108949560B - Cell climbing piece fixing device

Info

Publication number: CN108949560B
Application number: CN201810865427.6A
Authority: CN
Inventors: 肖凌飞; 姚诗怡
Original assignee: Wuhan University WHU
Current assignee: Wuhan University WHU
Priority date: 2018-08-01
Filing date: 2018-08-01
Publication date: 2020-06-09
Anticipated expiration: 2038-08-01
Also published as: CN108949560A

Abstract

The invention discloses a cell climbing sheet fixing device which comprises a short pipe and a plurality of tentacles, wherein the tentacles are fixedly connected to the lower edge of the short pipe, the number of the tentacles is two, namely a thick tentacle and a thin tentacle, the number of the thin tentacles is two, the thick tentacles is at least two, the thick tentacles and the thin tentacles are uniformly distributed, the cross section of each thick tentacle is in a fan-shaped shape, the bottom end of each thick tentacle is provided with a clamping groove, the clamping grooves are in fan-shaped shapes extending outwards from the inner side of each thick tentacle, the depth of each clamping groove is slightly smaller than the thickness of a climbing sheet, the clamping grooves of different thick tentacles can be combined to accommodate one climbing sheet to limit the movement of the climbing sheet, the thin tentacles have certain elasticity, and the distance. The periphery of the outer wall of the short pipe is provided with a bulge. The cell climbing sheet fixing device is simple in structure, and can well fix the cell climbing sheet in the culture hole at the bottom of the hole to prevent the cell climbing sheet from moving; the tentacle has the functions of limiting movement and clamping.

Description

Cell climbing piece fixing device

Technical Field

The invention relates to the field of experimental equipment, in particular to a cell climbing sheet fixing device.

Background

The cell slide is prepared by immersing a slide in a cell culture medium, and cells grow on the slide, and is mainly used for histology, immunohistochemistry, frozen sections, cell smear, in-situ hybridization and the like. In the actual cell slide experiment, a plurality of problems exist, for example, the contact between the slide and the bottom of a culture hole is not tight enough, and cells are easy to enter the bottom of the slide after inoculation so as to interfere the experiment; the slide is easy to move along with the shaking of the culture medium in the culture medium, so that the adherence and growth of cells are influenced; the climbing sheet is too thin and is not easy to clamp and take out; when a layer of biomembrane is laid on the climbing sheet for the experiment of inoculating cells on the biomembrane, the biomembrane is not easy to be fixed on the climbing sheet and is easy to float and move.

Disclosure of Invention

In order to overcome the defects of the prior art, the invention provides a cell slide fixing device which can well fix a cell slide at the bottom of a hole to prevent the cell slide from moving, and can press the biological membrane on the slide when a layer of biological membrane is paved on the slide for an experiment of inoculating cells on the biological membrane.

The technical scheme adopted by the invention is as follows:

a cell slide fixing device comprises a short pipe and a plurality of tentacles, wherein the tentacles are fixedly connected to the lower edge of the short pipe, the number of the tentacles is two, namely a thick tentacle and a thin tentacle, the number of the thin tentacles is two, the thick tentacles is at least two, the thick tentacles and the thin tentacles are uniformly distributed, the cross section of each thick tentacle is in a fan-shaped shape, the bottom end of each thick tentacle is provided with a clamping groove, the clamping groove is in a fan-shaped shape extending outwards from the inner side of the thick tentacle, the depth of the clamping groove is slightly smaller than the thickness of a slide, the distance from the inner edge of the clamping groove to a short pipe shaft is smaller than the radius of the slide, the distance from the outer edge of the clamping groove to the short pipe shaft is larger than the radius of the slide, the clamping grooves of different thick tentacles are combined to accommodate a slide and limit the movement of the slide, the thin tentacles have certain elasticity, the distance, the cell climbing film can be clamped by matching with the clamping groove under the natural state of the thin tentacle.

In the cell slide fixing device, the periphery of the outer wall of the short pipe is provided with the bulges.

The cross section of the thin tentacle is rectangular.

The cell climbing piece fixing device is characterized in that the number of the bulges is 4, the bulges are cylindrical, and the bulges are uniformly distributed on the periphery of the outer wall of the short pipe.

The invention has the advantages that;

1. the structure is simple, and the cell climbing sheet in the culture hole can be well fixed at the bottom of the hole to prevent the cell climbing sheet from moving;

2. the thick tentacles and the thin tentacles have the functions of limiting movement and clamping, and the thick tentacles and the thin tentacles are matched to clamp the climbing film, so that the climbing film is convenient to take out, and the climbing film is more beneficial to the experiment than the traditional tweezers;

3. the thin tentacles have elasticity, can be clamped more tightly and can adapt to the size of the climbing sheet;

4. the projections facilitate the placement of the climbing pieces in the middle of the culture hole, and experimental errors caused by different placement positions of the climbing pieces due to the thin short pipe are reduced;

5. the fine tentacle centre gripping is better than directly putting into with tweezers centre gripping because it can let the slide put in the centre of cultivateing the hole more conveniently, can reduce the inhomogeneous interference of cell inoculation like this.

Drawings

FIG. 1 is a schematic front view of the overall structure of the present invention;

FIG. 2 is an inverted schematic of the overall structure of the present invention;

FIG. 3 is a schematic view of the nature of the detail tentacle of the present invention;

FIG. 4 is a schematic view showing the state of the tentacle of the present invention being spread by tweezers;

FIG. 5 is a state reference diagram of the present invention in the practical use process when the creeper is placed upside down;

FIG. 6 is a reference view showing a state where a tentacle of the present invention grips a slide and places it in a culture well;

fig. 7 is a reference view of the successful placement of the fixation device of the present invention.

In the figure, 1-short pipe, 2-thick tentacle, 3-thin tentacle, 4-climbing piece, 5-tweezers, 6-culture hole, 7-clamping groove, 8-thin tentacle inner side, 9-clamping groove outer edge, 10-clamping groove inner edge and 11-bulge.

Detailed Description

The present invention is further illustrated by the following specific examples.

A cell slide fixing device comprises a short pipe 1 and a plurality of tentacles, the tentacles are fixedly connected to the lower edge of the short pipe 1, the number of the tentacles is two, namely a thick tentacle 2 and a thin tentacle 3, the number of the thin tentacles 3 is two, the thick tentacles 2 is at least two, the thick tentacles 2 and the thin tentacles 3 are uniformly distributed, the cross section of the thick tentacles 2 is in a sector ring shape, the bottom end of the thick tentacles 2 is provided with a clamping groove 7, the clamping groove 7 is in a sector ring shape extending outwards from the inner side of the thick tentacles 2, the depth of the clamping groove is slightly smaller than the thickness of a slide 4, the distance from the inner edge 10 of the clamping groove to the short pipe shaft is smaller than the radius of the slide, the distance from the outer edge 9 of the clamping groove to the short pipe shaft is larger than the radius of the slide 4 (slightly larger than the radius of the slide, so that the slide can be placed into a circular groove formed by combining, if too little tentacle is not enough to clip the climbing film), the height that highly less than or equal to the height of the thick tentacle top to the climbing film bottom after the card slot is put into to the height less than or equal to climbing film that the thin tentacle 3 highly is greater than the thick tentacle top of climbing film, promptly, the height that highly is not higher than the climbing film of thin tentacle subtracts the degree of depth of card slot (thin tentacle top and thick tentacle top parallel and level) from the height of thick tentacle, makes the thin tentacle natural state under can cooperate the card slot to clip the cell climbing film from width and length direction. The short pipe, the thick tentacle and the thin tentacle can be integrally formed by plastic.

Preferably, the periphery of the outer wall of the short pipe is provided with a bulge 11, and the distance from the bulge to the short pipe shaft is slightly smaller than the inner diameter of the culture hole.

Preferably, the thin tentacles 3 are rectangular in cross section (trapezoidal as well, rectangular being only one of them).

Preferably, the protrusions 11 are 4 in number, are cylindrical and are uniformly distributed on the periphery of the outer wall of the short pipe.

Optionally, the number of coarse tentacles is 6. The fixing device can design short pipes with different sizes according to culture holes with different sizes, and the number, the position and the length of tentacles on the pipes can also be designed according to actual requirements. According to the actual use condition, three types of the 6-hole plate, the 12-hole plate and the 24-hole plate are available. The specific dimensions of the fixing device are adapted to the dimensions of the culture well. Taking a 12-hole plate culture hole as an example, four bulges are arranged on the periphery of the short pipe 1, the distance between the far ends of the two opposite bulges is 22.4mm, and after the device is placed into the 12-hole plate culture hole (the inner diameter is 22.8mm), the bulges are abutted against the inner wall of the culture hole to limit the movement of the device. The short pipe has the inner diameter of 13mm, the outer diameter of 16mm and the height of 8mm, and the device is suitable for 12-hole plates. The six thick tentacles 2 and the two thin tentacles 3 are connected to the lower edge of the short pipe and are respectively positioned at eight corners of the regular octagon. Six thick tentacles 2 are 6mm highly, and the cross section is fan ring shape, and open the bottom has the draw-in groove, and draw-in groove degree of depth 0.15mm is less than 0.17mm of slide thickness a little. Two thin tentacles 3 highly are 6.02mm, and the cross section is the rectangle, is the symmetric distribution, because thinner, has certain elasticity, can be strutted by tweezers, and tweezers move away back thin tentacles and can carry the climbing piece, carry back length design and make the bottom can not protrusion in the climbing piece, and the climbing piece can in close contact with the culture hole. The cross section of the thin tentacle 3 can be rectangular or trapezoidal, and the like.

When the device is used, the tweezers 5 are compressed and stretched into the device, the two ends of the tweezers are propped against the thin tentacles 2 to be propped open, the device is inverted, the climbers 4 are placed in the clamping grooves at the bottom ends of the device tentacles, the tweezers 5 are slightly released, the thin tentacles 3 are used for clamping the climbers 4, and the device is placed in the culture hole 6.

Claims

1. A cell slide fixing device which is characterized in that: the short tube and the plurality of tentacles are fixedly connected to the lower edge of the short tube, the number of the tentacles is two, namely a thick tentacle and a thin tentacle, the number of the thin tentacles is two, the number of the thick tentacles is at least two, the thick tentacles and the thin tentacles are uniformly distributed, the cross section of the thick tentacles is in a fan-shaped shape, the bottom end of the thick tentacles is provided with a clamping groove, the clamping groove is in a fan-shaped shape extending outwards from the inner side of the thick tentacles, the depth of the clamping groove is slightly smaller than the thickness of a climbing piece, the distance from the inner edge of the clamping groove to a short tube shaft is smaller than the radius of the climbing piece, the distance from the outer edge of the clamping groove to the short tube shaft is larger than the radius of the climbing piece, the clamping grooves of different thick tentacles are combined to be capable of accommodating one climbing piece and limiting the movement of the climbing piece, the thin tentacl, the cell climbing film can be clamped by matching with the clamping groove under the natural state of the thin tentacle.

2. A cell-slide fixing device according to claim 1, wherein: and bulges are arranged on the periphery of the outer wall of the short pipe.

3. A cell-slide fixing device according to claim 1, wherein: the cross section of the thin tentacle is rectangular.

4. A cell-slide fixing device according to claim 2, wherein: the number of the bulges is 4, the bulges are cylindrical and are uniformly distributed on the periphery of the outer wall of the short pipe.