CN108949529B - Culture bottle liquid seal structure and method - Google Patents
Culture bottle liquid seal structure and method Download PDFInfo
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- CN108949529B CN108949529B CN201810682134.4A CN201810682134A CN108949529B CN 108949529 B CN108949529 B CN 108949529B CN 201810682134 A CN201810682134 A CN 201810682134A CN 108949529 B CN108949529 B CN 108949529B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
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Abstract
The invention discloses a culture flask liquid seal structure and a culture flask liquid seal method, and aims to solve the problem that when a culture flask is used for detecting seawater, manual regular water adding and liquid seal are needed, and the operation is inconvenient. The culture bottle comprises a culture bottle arranged in a culture box, wherein the culture bottle comprises a bottle body, a bottle neck and a bottle plug, a trumpet-shaped liquid sealing cup is arranged at the upper end of the bottle neck, the liquid sealing cup gradually enlarges from bottom to top, the inner wall of the bottle neck and the outer wall of the bottle plug gradually enlarge from bottom to top, the bottle plug is adaptive to and plugged into the bottle neck, and water sealing liquid is filled in the liquid sealing cup. After the solution to be detected is filled in the culture bottle, the bottle stopper is plugged into the bottle neck, the upper part of the bottle stopper is exposed out of the liquid sealing cup, and then water sealing liquid is filled in the liquid sealing cup; the culture flask is placed into an incubator for culture. The upper end of the bottleneck is provided with the horn-shaped liquid seal cup, and the liquid seal liquid filled in the liquid seal cup can meet the requirement of liquid seal for five days, so that the culture bottle is always kept in a water seal state during the culture, and the operation of detecting seawater by the culture bottle is greatly facilitated.
Description
Technical Field
The invention relates to a seawater detection technology, in particular to a culture flask liquid seal structure and a culture flask liquid seal method.
Background
At present, in the method of GB17378.4-2007, water samples in the seawater for determining the biochemical oxygen demand by the five-day culture method are kept at the sealing opening of a culture bottle all the time during the culture period, and the sealing opening can be covered on a bell mouth by paper or plastic to reduce the evaporation of the sealing opening water during the culture period. However, in the practical experiment process, the situation that the evaporation of the sealing water is reduced by using a paper or plastic cap can not meet the requirement of liquid sealing for five days is found, and the manual and timed water adding and liquid sealing are needed, so that the operation is very inconvenient.
Disclosure of Invention
The invention overcomes the defects that when a culture bottle is used for detecting seawater, manual and timed water adding and liquid sealing are needed, and the operation is inconvenient, and provides the culture bottle liquid sealing structure and the culture bottle liquid sealing method.
In order to solve the technical problems, the invention adopts the following technical scheme: the utility model provides a blake bottle liquid seals structure, is including arranging the blake bottle in the incubator in, and the blake bottle includes body, bottleneck, bottle plug, and the bottleneck upper end is equipped with the liquid seal cup that is loudspeaker form by lower up grow gradually, and bottleneck inner wall and bottle plug outer wall are all by lower up grow gradually, and the bottleneck is filled in to the bottle plug adaptation, is equipped with water seal liquid in the liquid seal cup.
After the solution to be detected is filled in the culture bottle, the bottle stopper is plugged into the bottle neck, the upper part of the bottle stopper is exposed out of the liquid sealing cup, and then water sealing liquid is filled in the liquid sealing cup; the culture flask is placed into an incubator for culture. The upper end of the bottleneck is provided with the horn-shaped liquid seal cup, and the liquid seal liquid filled in the liquid seal cup can meet the requirement of liquid seal for five days, so that the culture bottle is always kept in a water seal state during the culture, and the operation of detecting seawater by the culture bottle is greatly facilitated.
As preferred, install the roof that catchments above the blake bottle in the incubator, catchment on the roof and the blake bottle one-to-one be equipped with the downward convex drainage of a plurality of protruding arch, the circular cone column structure of big-end-up is drawn together to the drainage arch, and the circular arc transition between the protruding top edge of drainage and the roof that catchments, the protruding lower extreme of drainage is the arc, installs water fog generator on the incubator, and water fog generator communicates through the pipeline in the incubator.
After the culture bottle is placed in the incubator, the water mist generator is started periodically, water mist is conveyed into the incubator through the water mist generator, the water mist is gathered on the water collecting top plate and is guided to the position of the drainage protrusion, and the water mist drips into the liquid seal cup through the lower end of the drainage protrusion to prevent the water seal liquid in the liquid seal cup from drying up. The automatic addition of water seal liquid is realized through the structure, the phenomenon of dry-out of the water seal liquid is avoided, and the culture bottle is ensured to be always in a water seal state during culture.
Preferably, the upper end surface of the bottle stopper is inclined downwards from the middle to the edge. The structure arrangement is convenient for water drops to be drained into the liquid sealing cup through the upper end face of the bottle stopper.
In another scheme, a liquid storage cavity is arranged in the bottle plug, water seal liquid is filled in the liquid storage cavity, a siphon cotton core is arranged between the liquid storage cavity and the liquid seal cup, one end of the siphon cotton core is arranged in the water seal liquid in the liquid storage cavity, and the other end of the siphon cotton core is arranged above the water seal liquid in the liquid seal cup.
Before the culture bottle is placed into the incubator, water sealing liquid is filled in the liquid sealing cup and the liquid storage cavity, and after the culture bottle is placed into the incubator, water in the liquid storage cavity is continuously supplied to the liquid sealing cup under the siphon action of the siphon cotton core, so that the water sealing liquid in the liquid sealing cup is prevented from drying up during the culture.
Preferably, the bottle plug comprises a bottle plug body and a connecting column, the bottle plug body is of a cylindrical structure with an opening at the upper end and a closed lower end, the lower end of the connecting column is integrally formed at the bottom of the bottle plug body, and a liquid storage cavity is formed between the outer wall of the connecting column and the inner wall of the bottle plug body. This arrangement facilitates the arrangement of the reservoir.
Preferably, the connecting column is sleeved with a connecting sleeve, the connecting sleeve is provided with a flexible ring which extends outwards, the edge of the flexible ring is connected with a sealing cover in a sealing mode, and the sealing cover is supported at the upper end of the liquid sealing cup in an overlapping mode close to the outer edge.
The closing cap overlap joint supports in liquid seal cup upper end, and the gravity of self carries out the closing cap to liquid seal cup upper end, slows down the evaporation rate of water seal liquid, and the closing cap is connected on flexible ring, can not fill in the process of bottleneck to the bottle plug and have the constraint, ensures the leakproofness of bottle plug.
Preferably, a limiting convex ring is arranged on the connecting column and close to the lower part of the connecting sleeve. The arrangement of the limiting convex ring positions the sealing cover, so that the sealing cover is stably connected to the flexible ring all the time, and the bottle stopper can be conveniently plugged and opened.
Preferably, a flow guide surface which is inclined outwards from top to bottom is arranged between the upper end surface and the outer edge of the bottle stopper. The guide surface plays a good role in guiding water flow flowing upwards and downwards from the siphon cotton core.
Preferably, the upper end of the bottle stopper is connected with a stopper handle. The plug handle is convenient for plugging and opening the plug.
A liquid sealing method of a culture bottle liquid sealing structure comprises the first step, after a solution to be detected is filled in a culture bottle, a bottle plug is plugged into a bottle neck, a liquid sealing cup is exposed out of the upper part of the bottle plug, and then water sealing liquid is filled in the liquid sealing cup; and secondly, putting the culture bottle into an incubator, periodically starting a water mist generator, conveying water mist into the incubator through the water mist generator, collecting the water mist on a water collecting top plate, guiding the water mist to a drainage protrusion position, and dripping the water mist into the liquid seal cup through the lower end of the drainage protrusion to prevent the water seal liquid in the liquid seal cup from drying up.
Compared with the prior art, the invention has the beneficial effects that: the in-process that the incubator was carried out to the blake bottle is put into, and the water seal liquid can not wither, makes the blake bottle remain the water seal state throughout during the cultivation, does not need manual interpolation water seal liquid, has made things convenient for the operation that the blake bottle detected the sea water greatly.
Drawings
FIG. 1 is a schematic structural view of embodiment 1 of the present invention;
FIG. 2 is a schematic structural view of embodiment 2 of the present invention;
FIG. 3 is a partially enlarged schematic view of a culture flask according to example 2 of the present invention;
in the figure: 1. the culture box, 2, the blake bottle, 3, the body, 4, the bottleneck, 5, the bottle plug, 6, the liquid seal cup, 7, the roof that catchments, 8, the drainage is protruding, 9, the water smoke generator, 10, the stock solution chamber, 11, the siphon cotton core, 12, spliced pole, 13, adapter sleeve, 14, flexible ring, 15, closing cap, 16, spacing bulge loop, 17, water conservancy diversion face, 18, stopper handle.
Detailed Description
The technical scheme of the invention is further described in detail by the following specific embodiments in combination with the attached drawings:
example 1: a culture bottle liquid seal structure (see attached figure 1) comprises a culture bottle 2 arranged in a culture box 1, wherein the culture bottle comprises a bottle body 3, a bottle neck 4 and a bottle plug 5, the upper end of the bottle neck is provided with a liquid seal cup 6 which is horn-shaped and gradually enlarges from bottom to top, the inner wall of the bottle neck and the outer wall of the bottle plug are gradually enlarged from bottom to top, the bottle plug is adaptive to and plugged into the bottle neck, and water seal liquid is filled in the liquid seal cup. The roof 7 that catchments is installed to the blake bottle top in the incubator, catchments on the roof and the blake bottle one-to-one and is equipped with the protruding 8 of the downward convex drainage of a plurality of, the circular cone column structure of big-end-up is to the drainage arch, the protruding top edge of drainage and the circular arc transition of catchmenting between the roof, the protruding lower extreme of drainage is the arc, installs water fog generator 9 on the incubator, and water fog generator communicates through the pipeline in the incubator. Be equipped with a bottom plate and two roof that catchments in the incubator, the bottom plate setting is close to lower extreme position in the incubator, and a roof that catchments sets up in incubator top position, and another roof that catchments sets up between the roof that catchments at bottom plate and incubator top, and the blake bottle has all been placed with the roof that catchments in the centre on the bottom plate. The water mist generator is communicated with the cavity between the two water collecting top plates and the cavity between the bottom plate and the water collecting top plates through pipelines. The water mist generator is arranged below the bottom plate. Supporting blocks are arranged on the upper bottom plate of the inner wall of the incubator and below the water collecting top plate, and the bottom plate and the water collecting top plate are supported on the supporting blocks. The upper end surface of the bottle stopper is arranged from the middle to the edge in a downward inclination way. The upper end of the bottle stopper is connected with an annular stopper handle 18.
A liquid sealing method of a culture bottle liquid sealing structure comprises the first step, after a solution to be detected is filled in a culture bottle, a bottle plug is plugged into a bottle neck, a liquid sealing cup is exposed out of the upper part of the bottle plug, and then water sealing liquid is filled in the liquid sealing cup; and secondly, putting the culture bottle into an incubator, starting the water mist generator periodically, starting the incubator at intervals of 1 hour, running for 10 minutes, conveying water mist into the incubator through the water mist generator, collecting the water mist on the water collecting top plate, guiding the water mist to the position of the drainage protrusion, and dripping the water mist into the liquid seal cup through the lower end of the drainage protrusion to prevent the water seal liquid in the liquid seal cup from drying up.
Example 2: a culture bottle liquid seal structure (see attached figure 2 and attached figure 3) comprises a culture bottle 2 arranged in a culture box 1, wherein the culture bottle comprises a bottle body 3, a bottle neck 4 and a bottle plug 5, the upper end of the bottle neck is provided with a liquid seal cup 6 which is horn-shaped and gradually enlarges from bottom to top, the inner wall of the bottle neck and the outer wall of the bottle plug are gradually enlarged from bottom to top, the bottle plug is adaptive to be plugged in the bottle neck, and water seal liquid is filled in the liquid seal cup. The bottle stopper is provided with a liquid storage cavity 10, water seal liquid is filled in the liquid storage cavity, a siphon cotton core 11 is arranged between the liquid storage cavity and the liquid seal cup, one end of the siphon cotton core is arranged in the water seal liquid in the liquid storage cavity, and the other end of the siphon cotton core is arranged above the water seal liquid in the liquid seal cup. The siphon cotton core is of an annular structure, the lower end of the siphon cotton core is turned inwards and arranged at the bottom of the liquid storage cavity, and the upper end of the siphon cotton core is turned outwards and arranged above the water seal liquid in the liquid seal cup. The bottle plug comprises a bottle plug body and a connecting column 12, the bottle plug body is of a cylindrical structure with an opening at the upper end and a closed lower end, the lower end of the connecting column is integrally formed at the bottom of the bottle plug body, and a liquid storage cavity is formed between the outer wall of the connecting column and the inner wall of the bottle plug body. The connecting column is sleeved with a connecting sleeve 13, the connecting sleeve is two-clack and is convenient to install on the connecting column, an annular locking groove is formed in the connecting column, the outer locking groove of the connecting sleeve corresponds to a position to be connected with a hoop, the hoop tightly connects the connecting sleeve on the connecting column, a flexible ring 14 extending outwards is arranged on the connecting sleeve, the flexible ring has elasticity, the edge of the flexible ring is hermetically connected with a sealing cover 15, and the sealing cover is close to the outer edge and is supported at the upper end of the liquid sealing cup in an overlapping. The flexible ring comprises an upper layer and a lower layer, and the sealing cover is connected between the upper layer and the lower layer of the flexible ring. A limiting convex ring 16 is arranged on the connecting column and is close to the lower part of the connecting sleeve. A flow guide surface 17 which is obliquely arranged from top to bottom to outside is arranged between the upper end surface and the outer edge of the bottle plug.
Before the culture bottle is placed into the incubator, water sealing liquid is filled in the liquid sealing cup and the liquid storage cavity, and after the culture bottle is placed into the incubator, water in the liquid storage cavity is continuously supplied to the liquid sealing cup under the siphon action of the siphon cotton core, so that the water sealing liquid in the liquid sealing cup is prevented from drying up during the culture. The closing cap overlap joint supports in liquid seal cup upper end, and the gravity of self carries out the closing cap to liquid seal cup upper end, slows down the evaporation rate of water seal liquid, and the closing cap is connected on flexible ring, can not fill in the process of bottleneck to the bottle plug and have the constraint, ensures the leakproofness of bottle plug.
The embodiments described above are merely two preferred embodiments of the present invention, and are not intended to limit the present invention in any way, and other variations and modifications may be made without departing from the spirit and scope of the invention as defined in the appended claims.
Claims (7)
1. A kind of culture flask liquid seals the structure, including the culture flask placed in incubator, the culture flask includes body, bottleneck, bottle plug, its characteristic is, the upper end of bottleneck has liquid seal cups that become horn-shaped from bottom to top gradually, bottleneck inner wall and bottle plug outer wall are from bottom to top gradually enlarged, the bottle plug is inserted into bottleneck, the liquid seal cup is equipped with water sealing liquid;
a water collecting top plate is arranged above the culture bottle in the culture box, a plurality of drainage bulges protruding downwards are arranged on the water collecting top plate and the culture bottle in a one-to-one correspondence manner, the drainage bulges are in a conical structure with a large upper part and a small lower part, the upper edges of the drainage bulges are in arc transition with the water collecting top plate, the lower ends of the drainage bulges are in an arc shape, a water mist generator is arranged on the culture box, and the water mist generator is communicated into the culture box through a pipeline;
further, perhaps be equipped with the stock solution chamber in the bottle plug, stock solution intracavity is equipped with the water seal liquid, be equipped with the siphon cotton core between stock solution chamber and the liquid seal cup, siphon cotton core one end sets up in the water seal liquid of stock solution intracavity, the siphon cotton core other end sets up the water seal liquid top in the liquid seal cup, the bottle plug includes bottle plug body and spliced pole, the cover is equipped with the adapter sleeve on the spliced pole, be equipped with the flexible ring that outwards stretches out on the adapter sleeve, flexible ring edge sealing connection closing cap, the closing cap is close to the outward flange overlap joint and supports in the liquid seal cup upper end.
2. The culture flask liquid seal structure as claimed in claim 1, wherein the upper end face of the bottle stopper is inclined downwardly from the center toward the edge.
3. The culture flask liquid seal structure as claimed in claim 1, wherein the flask plug body is a cylindrical structure with an open upper end and a closed lower end, the lower end of the connecting column is integrally formed at the bottom of the flask plug body, and a liquid storage cavity is formed between the outer wall of the connecting column and the inner wall of the flask plug body.
4. The culture flask liquid seal structure as claimed in claim 1, wherein a limit convex ring is provided on the connection column near the lower part of the connection sleeve.
5. The structure as claimed in any one of claims 1 to 4, wherein a flow guide surface is provided between the top end of the bottle top and the outer edge, the flow guide surface being inclined outward from top to bottom.
6. The culture flask liquid seal structure as claimed in any one of claims 1 to 4, wherein a stopper handle is connected to an upper end of the stopper.
7. A method for liquid sealing a culture flask, which is carried out by using the liquid sealing structure of the culture flask according to claim 1, comprising the steps of: firstly, after a solution to be detected is filled in a culture bottle, a bottle plug is plugged into a bottle neck, a liquid seal cup is exposed out of the upper part of the bottle plug, and then water seal liquid is filled in the liquid seal cup; and secondly, putting the culture bottle into an incubator, periodically starting a water mist generator, conveying water mist into the incubator through the water mist generator, collecting the water mist on a water collecting top plate, guiding the water mist to a drainage protrusion position, and dripping the water mist into the liquid seal cup through the lower end of the drainage protrusion to prevent the water seal liquid in the liquid seal cup from drying up.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810682134.4A CN108949529B (en) | 2018-06-27 | 2018-06-27 | Culture bottle liquid seal structure and method |
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| CN201810682134.4A CN108949529B (en) | 2018-06-27 | 2018-06-27 | Culture bottle liquid seal structure and method |
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| CN108949529A CN108949529A (en) | 2018-12-07 |
| CN108949529B true CN108949529B (en) | 2021-06-22 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000228976A (en) * | 1999-02-12 | 2000-08-22 | Hiyoshi:Kk | Isothermal culture for measuring bod and apparatus therefor |
| CN203985937U (en) * | 2014-07-21 | 2014-12-10 | 宋维凯 | Sauerkraut altar altar saliva envelope automatic water-replenishing device |
| CN104782450A (en) * | 2015-05-18 | 2015-07-22 | 陈烁 | Drop irrigation vessel |
| CN205161270U (en) * | 2015-10-23 | 2016-04-20 | 大顺国际花卉股份有限公司 | Cultivate basin from inhaling formula flowers |
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2018
- 2018-06-27 CN CN201810682134.4A patent/CN108949529B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000228976A (en) * | 1999-02-12 | 2000-08-22 | Hiyoshi:Kk | Isothermal culture for measuring bod and apparatus therefor |
| CN203985937U (en) * | 2014-07-21 | 2014-12-10 | 宋维凯 | Sauerkraut altar altar saliva envelope automatic water-replenishing device |
| CN104782450A (en) * | 2015-05-18 | 2015-07-22 | 陈烁 | Drop irrigation vessel |
| CN205161270U (en) * | 2015-10-23 | 2016-04-20 | 大顺国际花卉股份有限公司 | Cultivate basin from inhaling formula flowers |
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| CN108949529A (en) | 2018-12-07 |
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