CN108947743A - A kind of preparation method based on Lumbrokinase-concave convex rod soil remediation fertilizer - Google Patents
A kind of preparation method based on Lumbrokinase-concave convex rod soil remediation fertilizer Download PDFInfo
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- CN108947743A CN108947743A CN201811186422.7A CN201811186422A CN108947743A CN 108947743 A CN108947743 A CN 108947743A CN 201811186422 A CN201811186422 A CN 201811186422A CN 108947743 A CN108947743 A CN 108947743A
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- lumbrokinase
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
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- Pest Control & Pesticides (AREA)
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Abstract
The present invention relates to a kind of preparation methods based on Lumbrokinase-concave convex rod soil remediation fertilizer；Attapulgite raw ore is weighed, distilled water immersion is added in the powder of crushed after being dried to 120~200 mesh；It weighs calgon dispersing agent to be added in concave convex rod powder aqueous solution, the quality of calgon is the 1%~4% of concave convex rod quality；Then it stirs, filter, obtain wet filter cake；Wet filter cake is added in Lumbrokinase extracting solution, vacuum freeze drying is carried out after mixed liquor stirring, finally crushes and be granulated to obtain Lumbrokinase-concave convex rod soil remediation fertilizer.The combination of concave convex rod and Lumbrokinase can greatly promote the utilization efficiency of Lumbrokinase soil remediation fertilizer, rehabilitating soil is inorganic or organic contamination, mitigate damage of the chemical fertilizer to soil, improve the soil environment of crop growth, fertilizer conservation water conservation, beneficial microelement is provided for the growth and development of crops, is a kind of green safe, environmentally friendly soil remediation fertilizer.
Description
Technical field
The invention belongs to field of biotechnology, specifically relate generally to a kind of Asia that Lumbrokinase is extracted using dimethyl ether as extractant
The preparation method of supercritical fluid extraction method and Lumbrokinase soil remediation fertilizer.
Background technique
With the progress of modern agriculture, fertilizer becomes a kind of essential means of production.But fertilizer is long-term
Using soil health is easily destroyed, soil hardening, acidification and salinization of soil are caused, weakens plant to fertilizer absorbability, agricultural production
Product quality is low, the low return of high investment.It can also make that food, toxic component increases in feed and drinking water, human and livestock health is caused to dive
It is endangering.Soil remediation fertilizer is applied, not only can increase the soil organism, edaphon improves soil texture, improves crop
Resistance, while also can be reduced the waste of fertilizer, potassium, phosphorus and high-quality nitrogen source needed for supplementing soil avoid making soil environment
It is the best control measure of chemical fertilizer in soil pollution at pollution.
Earthworm, invertebrate belong to Annelida Oligochaeta.It is known as " pheretima " in traditional Chinese medicine, pheretima is cold in nature, taste is micro-
Hardship, have heat-clearing arresting convulsion, dredging collateral, relieving asthma, diuresis the effect of, be traditional Chinese medicine.Earthworm enteron aisle can secret out of many special
A variety of biological enzyme such as enzyme, including cellulase, chitinase, protease, amylase, peroxidase, these native enzyme systems
Agent activity is high, decomposition can be cooperateed with to rot organic matter with microorganism, and can effectively prevent pathogen to soil and crops
Harm.Currently, research mainly still concentrates on the extraction separation aspect of Lumbrokinase.Lumbrokinase is that molecular weight is 16000-
The multicomponent protease of 50000KD, and be a kind of organic substance, earthworm amount and micro- life in soil can be promoted as Fertilizer application
The increase of object amount, while can effectively activate, chesson increases the content of soil with organic matter, improve soil texture and
Physicochemical property promotes absorption of the root system to nutrient.Concave convex rod also known as palygorskite are a kind of layer of chain transition structures containing Shuifu County
Clay pit based on magnesium silicate.The characteristics of concave convex rod huge specific surface determine its with good absorption property, it is unique
Club shaped structure and porous permeability be the key that improvement soil.Concave convex rod is not fertilizer, uses in agricultural production
Make fertilizer additive.Lumbrokinase and concave convex rod are mixed with soil remediation fertilizer, natural fertilizers obtained can improve soil, mention
High soil activation improves quality of agricultural product, reduces Soil Environmental Pollution.
CN105907738A discloses a kind of method that Lumbrokinase is extracted in such a way that segmentation is saltoutd.Earthworm in the method
Homogenate needs to stand overnight after ammonium sulfate precipitation is handled, and consuming time is long, and extraction efficiency is low, the enzymatic activity and purity of acquisition
It is not high, it is not suitable for industrialized production.CN105130697A discloses a kind of preparation method of earthworm enzyme soil remediation fertilizer, the reparation
Fertilizer includes original fertilizer and earthworm enzyme.The method requires strictly, although to a certain extent the content of nitrogen and phosphorous in original fertilizer
Can improve the utilization rate of fertilizer, but still can some unemployed fertilizer enter soil, long-term accumulation and conversion, cause
Soil pollution.
Summary of the invention
The purpose of the present invention is being the shortcomings that overcoming prior art, a kind of system of Lumbrokinase soil remediation fertilizer is mainly provided
Preparation Method；The subcritical fluid extraction method that Lumbrokinase is extracted using dimethyl ether as extractant is proposed simultaneously；The extracting process mentions
Take speed fast, high-efficient, product no solvent residue, the Lumbrokinase component extracted is more, and magazine content is few, enzyme is higher than work, can be real
Existing industrialization large-scale production；The Lumbrokinase of preparation-concave convex rod soil remediation fertilizer can greatly improve soil environment, improve agriculture
Crop yield and quality.
The purpose of the present invention is realized by following scheme:
A kind of preparation method based on Lumbrokinase-concave convex rod soil remediation fertilizer；Include the following steps:
1) attapulgite raw ore is weighed, distilled water immersion is added in the powder of crushed after being dried to 120~200 mesh；
2) it weighs calgon dispersing agent to be added in concave convex rod powder aqueous solution, the quality of calgon is bumps
The 1%~4% of stick quality；Then it stirs, filter, obtain wet filter cake；
3) wet filter cake is added in Lumbrokinase extracting solution, carries out vacuum freeze drying, final powder after mixed liquor stirring
Broken and granulation obtains Lumbrokinase-concave convex rod soil remediation fertilizer.
The preferred steps 1) in 1~3h of distilled water immersion is added, the solid-to-liquid ratio of concave convex rod and distilled water is 1:2~1:4
(kg:L)。
The preferred steps 2) 60~100min of stirring, it filters and with 100~500mL distilled water flushing filter cake, filter cake
Moisture content is 3%~17%.
The preferred steps 3) and wet filter cake is added in Lumbrokinase extracting solution, wet concave convex rod and Lumbrokinase extract
The solid-to-liquid ratio of liquid is 1:5~1:15 (g:mL)
The preferred steps 3) mixed liquor carries out vacuum freeze drying after stirring 10~20min, -80 DEG C of cryogenic temperature~-
60 DEG C, drying time is 8~12h.
Lumbrokinase extracting solution of the invention can be prepared using art methods, or directly be bought, while can use
Following method preparation: method includes the following steps:
1) it weighs fresh earthworm flowing water to rinse, its intracorporal pollutant is discharged as far as possible；The earthworm after cleaning is taken to blot water
Point, adding 0.1mol/L, pH is 6.5~8.5 phosphate buffer (PBS), and homogenate obtains earthworm homogenate；
2) gained earthworm homogenate is stood, is then centrifuged in centrifuge, discards precipitating, take supernatant；By supernatant
It puts into subcritical abstraction kettle, closed extraction kettle, is passed through the dimethyl ether of 0.7~1L volume, the temperature in adjusting extraction kettle to 20
~55 DEG C, pressure to 0.51~1.31Mpa guarantees extractant with the presence of subcritical fluids state, continuous extraction；
3) after extracting, extract liquor is put into solvent recovery kettle, closed extraction kettle is passed through dimethyl ether, and keeps temperature
40~60 DEG C, it is decompressed to 0.13~1.26Mpa, the gasification of dimethyl ether subcritical fluids, recycling design obtains Lumbrokinase extracting solution.
The solid-to-liquid ratio of fresh earthworm and phosphate buffer (PBS) are 1:3~1:5 (kg:L) during the step 1) is preferred, even
5min~8min is starched, earthworm homogenate is obtained.
After gained earthworm homogenate is preferably stood 2~4h by the step 2), immediately in centrifuge with 5000~
10000r/min is centrifuged 10~15min.
The step 2) preferably 40~260min of continuous dynamic extraction.
Compared with existing Lumbrokinase extracting method and Lumbrokinase soil remediation fertilizer preparation method, the present invention has following excellent
Gesture:
(1) Lumbrokinase subcritical fluid extraction technology extraction rate is fast, high-efficient, product no solvent residue, can retain and mention
It takes the active constituent of object not destroy not aoxidizing, continuous production is good, and the Lumbrokinase component extracted is more, enzymatic activity and purity
Height is, it can be achieved that industrialization large-scale production.
(2) combination of concave convex rod and Lumbrokinase can greatly promote the utilization efficiency of Lumbrokinase soil remediation fertilizer, repair
Soil inorganic or organic contamination mitigate damage of the chemical fertilizer to soil, improve the soil environment of crop growth, and fertilizer conservation water conservation is
The growth and development of crops provides beneficial microelement, is a kind of green safe, environmentally friendly soil remediation fertilizer.
Repairing effect test experience of the Lumbrokinase-concave convex rod soil remediation fertilizer to chronic administration chemical fertilizer soil, detailed process
Are as follows:
Sample is Tianjin Wuqing District green house of vegetables soil, carries out laboratory research.Randomly select in greenhouse three pieces not
Soil with region is sampled as sampling unit, is denoted as A, B, C.1kg soil successively is weighed from each sampling unit before fertilising
Sample is measured, and measurement result is denoted as A1, B1, C1 as control.It is then respectively adding earthworm prepared in Examples 1 to 3
Kinases-concave convex rod soil remediation fertilizer, is measured, measurement result is denoted as A2, B2, C2 after two weeks.
Weighed soil sample is detected after pretreatment.Soil sample PH is measured using PH meter；Soil sample organic matter, nitrogen, phosphorus,
Potassium is detected using soil nutrient tacheometer；Soil sample Cr content is measured using atomic absorption spectrography (AAS).Obtained measurement
As a result as shown in the table:
It is from the above testing result it is found that good by the soil remediation fertilizer repairing effect of this method preparation, have adjusted soil
PH value, organic matter is increased, supplemented with phosphorus, potassium and high-quality nitrogen source required for soil, additionally, due to the absorption of concave convex rod
Effect, so that this remediation fertilizer also has good removal effect to heavy metal-polluted soil.
Specific embodiment
Added Lumbrokinase-concave convex rod soil remediation fertilizer in soil sample A1, B1, C1 described in above-mentioned repairing effect test experience
The preparation being respectively derived from following embodiments 1,2,3 further explains the present invention below by specific case study on implementation in detail
It states.
Embodiment 1
1) attapulgite raw ore, the powder of crushed after being dried to 120 mesh are weighed；Be added distilled water immersion 1h, concave convex rod with
The solid-to-liquid ratio of distilled water is 1:2 (kg:L)；
2) it weighs calgon dispersing agent to be added in concave convex rod powder aqueous solution, the quality of calgon is bumps
The 1% of stick quality；Then stir 60min, filter and with 100mL distilled water flushing filter cake, obtain wet filter cake, filter cake it is aqueous
Rate is 3%；
3) wet filter cake is added in Lumbrokinase extracting solution, the solid-to-liquid ratio of wet concave convex rod and Lumbrokinase extracting solution is 1:
5(g:mL)；Will the mixed liquor stir 10min after carry out vacuum freeze drying, -80 DEG C of cryogenic temperature, drying time 8h, finally
It crushes and is granulated to obtain Lumbrokinase-concave convex rod soil remediation fertilizer.
The Lumbrokinase extracting solution of implementation can be prepared using art methods, or directly be bought, while can be using such as
The preparation of lower section method: method includes the following steps:
1) it weighs fresh earthworm flowing water to rinse, its intracorporal pollutant is discharged as far as possible；The earthworm after cleaning is taken to blot water
Point, adding 0.1mol/L, pH is 6.5 phosphate buffer (PBS), and fresh earthworm and the solid-to-liquid ratio of phosphate buffer (PBS) are
1:3(kg:L)；It is homogenized 5min, obtains earthworm homogenate；
2) after gained earthworm homogenate being stood 2h, 10min is then centrifuged with 5000r/min in centrifuge, it is heavy to discard
It forms sediment, takes supernatant；Supernatant is put into subcritical abstraction kettle, closed extraction kettle, be passed through the dimethyl ether of 0.7L volume, adjusts extraction
Take the temperature in kettle to 20 DEG C, pressure to 0.51Mpa guarantees extractant with the presence of subcritical fluids state, continuous extraction
40min；
3) after extracting, extract liquor is put into solvent recovery kettle, closed extraction kettle, and kept for 40 DEG C of temperature, be decompressed to
0.13Mpa, the gasification of dimethyl ether subcritical fluids, recycling design obtain Lumbrokinase extracting solution.
Lumbrokinase made from embodiment 1-concave convex rod soil remediation fertilizer is added in A soil sample, is measured after two weeks, measurement knot
Fruit is denoted as A2；It is compared with original soil sample measured value A1, the results showed that, Lumbrokinase-concave convex rod soil remediation fertilizer is effectively adjusted
The pH value of soil sample A, increases organic matter, supplemented with phosphorus, potassium and high-quality nitrogen source required for soil, to heavy metal-polluted soil
There is certain removal effect.
Embodiment 2
1) attapulgite raw ore, the powder of crushed after being dried to 150 mesh are weighed；Be added distilled water immersion 2h, concave convex rod with
The solid-to-liquid ratio of distilled water is 1:3 (kg:L)；
2) it weighs calgon dispersing agent to be added in concave convex rod powder aqueous solution, the quality of calgon is bumps
The 2% of stick quality；Then stir 80min, filter and with 300mL distilled water flushing filter cake, obtain wet filter cake, filter cake it is aqueous
Rate is 9%；
3) wet filter cake is added in Lumbrokinase extracting solution, the solid-to-liquid ratio of wet concave convex rod and Lumbrokinase extracting solution is 1:
10(g:mL)；Will the mixed liquor stir 15min after carry out vacuum freeze drying, -72 DEG C of cryogenic temperature, drying time 9h, most
After crush and be granulated to obtain Lumbrokinase-concave convex rod soil remediation fertilizer.
The Lumbrokinase extracting solution of implementation can be prepared using art methods, or directly be bought, while can be using such as
The preparation of lower section method: method includes the following steps:
1) it weighs fresh earthworm flowing water to rinse, its intracorporal pollutant is discharged as far as possible；The earthworm after cleaning is taken to blot water
Point, adding 0.1mol/L, pH is 7.2 phosphate buffer (PBS), and fresh earthworm and the solid-to-liquid ratio of phosphate buffer (PBS) are
1:4(kg:L)；It is homogenized 6min, obtains earthworm homogenate；
2) after gained earthworm homogenate being stood 2.5h, 10min is then centrifuged with 8000r/min in centrifuge, is discarded
Precipitating, takes supernatant；Supernatant is put into subcritical abstraction kettle, closed extraction kettle is passed through the dimethyl ether of 0.8L volume, adjusts
For temperature in extraction kettle to 42 DEG C, pressure to 1.18Mpa guarantees extractant with the presence of subcritical fluids state, continuous extraction
180min；
3) after extracting, extract liquor is put into solvent recovery kettle, closed extraction kettle, and kept for 55 DEG C of temperature, be decompressed to
0.86Mpa, the gasification of dimethyl ether subcritical fluids, recycling design obtain Lumbrokinase extracting solution.
Lumbrokinase made from embodiment 2-concave convex rod soil remediation fertilizer is added in B soil sample, is measured after two weeks, measurement knot
Fruit is denoted as B2；It is compared with original soil sample measured value B1, the results showed that, Lumbrokinase-concave convex rod soil remediation fertilizer is effectively adjusted
The pH value of soil B, increases organic matter, supplemented with phosphorus, potassium and high-quality nitrogen source required for soil, to heavy metal-polluted soil
There is certain removal effect.
Embodiment 3
1) attapulgite raw ore, the powder of crushed after being dried to 200 mesh are weighed；Be added distilled water immersion 3h, concave convex rod with
The solid-to-liquid ratio of distilled water is 1:4 (kg:L)；
2) it weighs calgon dispersing agent to be added in concave convex rod powder aqueous solution, the quality of calgon is bumps
The 4% of stick quality；Then it stirs 100min, filters and with 500mL distilled water flushing filter cake, obtain wet filter cake, filter cake contains
Water rate is 17%；
3) wet filter cake is added in Lumbrokinase extracting solution, the solid-to-liquid ratio of wet concave convex rod and Lumbrokinase extracting solution is 1:
15(g:mL)；Will the mixed liquor stir 20min after carry out vacuum freeze drying, -60 DEG C of cryogenic temperature, drying time 12h, most
After crush and be granulated to obtain Lumbrokinase-concave convex rod soil remediation fertilizer.
The Lumbrokinase extracting solution of implementation can be prepared using art methods, or directly be bought, while can be using such as
The preparation of lower section method: method includes the following steps:
1) it weighs fresh earthworm flowing water to rinse, its intracorporal pollutant is discharged as far as possible；The earthworm after cleaning is taken to blot water
Point, adding 0.1mol/L, pH is 8.5 phosphate buffer (PBS), and fresh earthworm and the solid-to-liquid ratio of phosphate buffer (PBS) are
1:5(kg:L)；It is homogenized 8min, obtains earthworm homogenate；
2) after gained earthworm homogenate being stood 4h, 15min is then centrifuged with 10000r/min in centrifuge, it is heavy to discard
It forms sediment, takes supernatant；Supernatant is put into subcritical abstraction kettle, closed extraction kettle, be passed through the dimethyl ether of 1L volume, adjusts extraction
For temperature in kettle to 55 DEG C, pressure to 1.31Mpa guarantees extractant with the presence of subcritical fluids state, continuous extraction 260min；
3) after extracting, extract liquor is put into solvent recovery kettle, closed extraction kettle, and keep temperature 60 C, be decompressed to
1.26Mpa, the gasification of dimethyl ether subcritical fluids, recycling design obtain Lumbrokinase extracting solution.
Lumbrokinase made from embodiment 3-concave convex rod soil remediation fertilizer is added in C soil sample, is measured after two weeks, measurement knot
Fruit is denoted as C2；It is compared with original soil sample measured value C1, the results showed that, Lumbrokinase-concave convex rod soil remediation fertilizer is effectively adjusted
The pH value of soil C, increases organic matter, supplemented with phosphorus, potassium and high-quality nitrogen source required for soil, to heavy metal-polluted soil
There is certain removal effect.
A kind of preparation method based on Lumbrokinase-concave convex rod soil remediation fertilizer that the present invention is disclosed and proposed, this field
Technical staff can be by using for reference present disclosure, and the appropriate links such as condition route that change are realized, although method and technology of the invention is
It is described by preferred embodiment, related technical personnel can obviously not depart from the content of present invention, in spirit and scope
Methods and techniques described herein route is modified or is reconfigured, to realize final technology of preparing.Especially need to refer to
Out, all similar replacements and change are apparent to those skilled in the art, they are considered as wrapping
It includes in spirit of that invention, range and content.
Claims (9)
1. a kind of preparation method based on Lumbrokinase-concave convex rod soil remediation fertilizer；It is characterized in that including the following steps:
1) attapulgite raw ore is weighed, distilled water immersion is added in the powder of crushed after being dried to 120~200 mesh；
2) it weighs calgon dispersing agent to be added in concave convex rod powder aqueous solution, the quality of calgon is concave convex rod matter
The 1%~4% of amount；Then it stirs, filter, obtain wet filter cake；
3) wet filter cake is added in Lumbrokinase extracting solution, carries out vacuum freeze drying after mixed liquor stirring, finally crushes simultaneously
Granulation obtains Lumbrokinase-concave convex rod soil remediation fertilizer.
2. the method as described in claim 1, it is characterized in that 1~3h of distilled water immersion is added in step 1), concave convex rod and distillation
The solid-to-liquid ratio of water is 1:2~1:4 (kg:L).
3. the method as described in claim 1 is filtered and is steamed with 100~500mL it is characterized in that step 2) stirs 60~100min
Distilled water rinses filter cake, and the moisture content of filter cake is 3%~17%.
4. the method as described in claim 1, it is characterized in that step 3) and wet filter cake is added in Lumbrokinase extracting solution, it is wet
Concave convex rod and Lumbrokinase extracting solution solid-to-liquid ratio be 1:5~1:15 (g:mL).
5. the method as described in claim 1, it is characterized in that progress vacuum refrigeration is dry after step 3) mixed liquor stirs 10~20min
Dry, -80 DEG C~-60 DEG C of cryogenic temperature, drying time is 8~12h.
6. the method as described in claim 1 includes the following steps: it is characterized in that Lumbrokinase extracts liquid and preparation method thereof
1) it weighs fresh earthworm flowing water to rinse, its intracorporal pollutant is discharged as far as possible；The earthworm suck dry moisture after cleaning is taken, is added
The phosphate buffer (PBS) that 0.1mol/L, pH are 6.5~8.5, homogenate, obtains earthworm homogenate；
2) gained earthworm homogenate is stood, is then centrifuged in centrifuge, discards precipitating, take supernatant；Supernatant is put into
In subcritical abstraction kettle, closed extraction kettle is passed through the dimethyl ether of 0.7~1L volume, the temperature in adjusting extraction kettle to 20~55
DEG C, pressure to 0.51~1.31Mpa guarantees extractant with the presence of subcritical fluids state, continuous extraction；
3) after extracting, extract liquor is put into solvent recovery kettle, closed extraction kettle is passed through dimethyl ether, and keep temperature 40~
60 DEG C, it is decompressed to 0.13~1.26Mpa, the gasification of dimethyl ether subcritical fluids, recycling design obtains Lumbrokinase extracting solution.
7. method as claimed in claim 6, it is characterized in that fresh earthworm and phosphate buffer (PBS) consolidate in the step 1)
Liquor ratio is 1:3~1:5 (kg:L), is homogenized 5min~8min, obtains earthworm homogenate.
8. method as claimed in claim 6, it is characterized in that step 2) exists immediately by after 2~4h of gained earthworm homogenate standing
10~15min is centrifuged with 5000~10000r/min in centrifuge.
9. method as claimed in claim 6, it is characterized in that 40~260min of the continuous dynamic extraction of step 2).
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111004633A (en) * | 2019-11-11 | 2020-04-14 | 王永强 | Lumbrokinase soil remediation agent and preparation method thereof |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103086803A (en) * | 2013-02-28 | 2013-05-08 | 中国科学院遗传与发育生物学研究所农业资源研究中心 | Lumbrukinase compound fertilizer and preparation method thereof |
| CN104788263A (en) * | 2015-04-16 | 2015-07-22 | 王龙潮 | Composite soil conditioner |
| CN105272765A (en) * | 2015-11-26 | 2016-01-27 | 国家粳稻工程技术研究中心 | Special corn field fertilizer prepared from wormcast |
| CN105294350A (en) * | 2015-11-26 | 2016-02-03 | 天津天隆农业科技有限公司 | Soil conditioner prepared by lumbrukinase special for paddy field and production technology thereof |
| CN105801295A (en) * | 2016-03-08 | 2016-07-27 | 李骁华 | Soil regenerant and preparation method thereof |
| CN105907738A (en) * | 2016-07-06 | 2016-08-31 | 奥为（天津）环保科技有限公司 | Method for efficiently extracting lumbrokinase from earthworms |
| CN106397012A (en) * | 2016-08-30 | 2017-02-15 | 何晓东 | Bio-enzyme doped soil remediation fertilizer and preparation method thereof |
| CN106613470A (en) * | 2016-09-30 | 2017-05-10 | 山东农业大学 | Alkalinity-substracting planting material applicable for vegetation-growing concrete and preparation method thereof |
| CN107189787A (en) * | 2017-06-07 | 2017-09-22 | 天津天丰泽田生物科技有限公司 | A kind of soil conditioner and preparation method thereof |
| CN107324943A (en) * | 2017-06-23 | 2017-11-07 | 天津天丰泽田生物科技有限公司 | A kind of rice seedling raising soil containing chitosan |
-
2018
- 2018-10-12 CN CN201811186422.7A patent/CN108947743A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103086803A (en) * | 2013-02-28 | 2013-05-08 | 中国科学院遗传与发育生物学研究所农业资源研究中心 | Lumbrukinase compound fertilizer and preparation method thereof |
| CN104788263A (en) * | 2015-04-16 | 2015-07-22 | 王龙潮 | Composite soil conditioner |
| CN105272765A (en) * | 2015-11-26 | 2016-01-27 | 国家粳稻工程技术研究中心 | Special corn field fertilizer prepared from wormcast |
| CN105294350A (en) * | 2015-11-26 | 2016-02-03 | 天津天隆农业科技有限公司 | Soil conditioner prepared by lumbrukinase special for paddy field and production technology thereof |
| CN105801295A (en) * | 2016-03-08 | 2016-07-27 | 李骁华 | Soil regenerant and preparation method thereof |
| CN105907738A (en) * | 2016-07-06 | 2016-08-31 | 奥为（天津）环保科技有限公司 | Method for efficiently extracting lumbrokinase from earthworms |
| CN106397012A (en) * | 2016-08-30 | 2017-02-15 | 何晓东 | Bio-enzyme doped soil remediation fertilizer and preparation method thereof |
| CN106613470A (en) * | 2016-09-30 | 2017-05-10 | 山东农业大学 | Alkalinity-substracting planting material applicable for vegetation-growing concrete and preparation method thereof |
| CN107189787A (en) * | 2017-06-07 | 2017-09-22 | 天津天丰泽田生物科技有限公司 | A kind of soil conditioner and preparation method thereof |
| CN107324943A (en) * | 2017-06-23 | 2017-11-07 | 天津天丰泽田生物科技有限公司 | A kind of rice seedling raising soil containing chitosan |
Non-Patent Citations (6)
| Title |
|---|
| 刘月蓉等: "亚临界萃取设备在天然产物有效成分提取中的应用", 《莆田学院学报》 * |
| 崔永丽等: "凹凸棒土的纯化及吸附性能研究 ", 《中国非金属矿工业导刊》 * |
| 王永华等: "《食品风味化学》", 30 June 2015, 中国轻工业出版社 * |
| 袁东振等: "用亚临界芝麻粕制备芝麻蛋白的工艺研究 ", 《河南工业大学学报(自然科学版)》 * |
| 郎跃深等: "《蚯蚓养殖技术与应用》", 28 February 2009, 科学技术文献出版社 * |
| 鲍士旦: "《土壤农化分析 第三版》", 31 March 1981, 中国农业出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111004633A (en) * | 2019-11-11 | 2020-04-14 | 王永强 | Lumbrokinase soil remediation agent and preparation method thereof |
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