CN108939159A - Small intestine acellular matrix film combination metal mesh wall of the chest sticking patch preparation method - Google Patents
Small intestine acellular matrix film combination metal mesh wall of the chest sticking patch preparation method Download PDFInfo
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- CN108939159A CN108939159A CN201810839040.3A CN201810839040A CN108939159A CN 108939159 A CN108939159 A CN 108939159A CN 201810839040 A CN201810839040 A CN 201810839040A CN 108939159 A CN108939159 A CN 108939159A
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- Prior art keywords
- metal mesh
- small intestine
- matrix film
- acellular matrix
- chest
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
- A61L27/3629—Intestinal tissue, e.g. small intestinal submucosa
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/02—Inorganic materials
- A61L27/04—Metals or alloys
- A61L27/06—Titanium or titanium alloys
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3641—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the site of application in the body
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/40—Preparation and treatment of biological tissue for implantation, e.g. decellularisation, cross-linking
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Engineering & Computer Science (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Veterinary Medicine (AREA)
- Dermatology (AREA)
- Medicinal Chemistry (AREA)
- Transplantation (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Botany (AREA)
- Vascular Medicine (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Zoology (AREA)
- Inorganic Chemistry (AREA)
- Prostheses (AREA)
- Materials For Medical Uses (AREA)
Abstract
The present invention provides a kind of small intestine acellular matrix film combination metal mesh wall of the chest sticking patch preparation method, belongs to medical biomaterial technical field.This method comprises: producing out small intestine acellular matrix film after the small intestine intercepted in animals is handled, then small intestine acellular matrix film is produced into out wall of the chest sticking patch in conjunction with metal mesh.Preparation method of the invention is simple, and obtained wall of the chest patching material is repaired the wall of the chest as chest wall defect patient and used.
Description
Technical field
The invention belongs to biomaterial for medical purpose fields, and in particular to a kind of small intestine acellular matrix film combination metal mesh wall of the chest
Sticking patch preparation method.
Background technique
There is chest wall defect in many severe trauma patients in clinic, be easy to cause pendular movement of mediastinum when defect area is larger,
Jeopardize patient vitals, routine operation is sectional repair, or the tissue at other positions of body or a part of organ is taken to carry out
Repairing, causes certain damage again while curing the disease.With the correlative study of small intestine acellular matrix film (ECM) biological sticking patch
Progress, my Research Team has developed a kind of small intestine acellular matrix film combination metal mesh wall of the chest patching material, and the material is clinical
It has wide range of applications, to be applied to the reparation of pleura, the wall of the chest, skull etc..Domestic relevant application study is more, but not yet faces
Bed application.
Summary of the invention
The purpose of the invention is to provide a kind of small intestine acellular matrix film combination metal mesh wall of the chest sticking patch preparation method,
The preparation method is simple for this, can produce to obtain wall of the chest patching material by this method.
Present invention firstly provides a kind of small intestine acellular matrix film combination metal mesh wall of the chest sticking patch preparation methods:
Step 1: acellular matrix film is produced with the small intestine intercepted in animals；
Step 2: different size of metal mesh is produced using titanium nickel alloy material, metal mesh is in slightly to protrude to plane side
Arachnoid Ti-Ni alloy metal mesh；
Step 3: the small intestine acellular matrix film that step 1 obtains is fixed on the metal mesh that step 2 obtains, is obtained
Wall of the chest patching material；
Step 4: the wall of the chest patching material that step 3 is obtained is through linear accelerating with 8000 ladd radiological dose radiation sterilizations
After be sealed.
Preferably, the animal of the step one is pig, ox, sheep, mouse, horse.
Preferably, the metal of the step one is Ti-Ni alloy.
Preferably, the acellular matrix film of the step one is that the small intestine intercepted with living animal passes through preparation
It obtains.
Preferably, the step two metal mesh is produced specifically: using titanium nickel alloy material produces different size
Metal mesh, the advantages of metal mesh is in the web-like structure that slightly protrudes to plane side, the structure is, with certain toughness and
Elasticity, and stable structure is unlikely to deform, and cannot be changed with thoracic cavity internal and external pressure difference after being implanted into the wall of the chest and be generated obvious activity.
Preferably, the acellular matrix film of the step three and the combination of metal mesh specifically: obtain step 1
Small intestine acellular matrix film be fixed on the metal mesh that step 2 obtains, obtain wall of the chest sticking patch.
Preferably, the step four freeze temperature is -80 DEG C, and freeze-drying time is for 24 hours.
Preferably, the sterilizing of the step four is that the wall of the chest patching material that will be sealed is drawn through linear accelerating with 8000
It is saved after moral radiological dose radiation sterilization.
The present invention provides the wall of the chest sticking patch material of small intestine acellular matrix film that above-mentioned preparation method obtains in conjunction with metal mesh
The preparation method of material.
Beneficial effects of the present invention
Present invention firstly provides a kind of small intestine acellular matrix film combination metal mesh wall of the chest sticking patch preparation method, wherein metal
Material is titanium nickel alloy material, and side reaction caused by the material is few, has been widely used for the reparation of human body or as fracture
Fixed device is applied to human body.Slightly concave sunken round reticular structure is put centered on the metal mesh, the structural stability is more preferable, is not easy
Deformation can resist bigger tension with less material, reduce influence of the metallics to human body.Acellular matrix film and metal
The combination of net is that metal mesh is fixed on specific holder device, by small intestine acellular matrix film along the straight of circular metal net
Diameter tiles and is wound in the two sides of metal mesh, can be closely linked with metal mesh.
Detailed description of the invention
Fig. 1 is the photo figure for the natural tubulose chitterlings acellular matrix film of single layer that the present invention produces.
Fig. 2 is before the single layer natural tubulose chitterlings acellular matrix film of the invention produced is dry in conjunction with metal mesh
Photo figure.
Fig. 3 is after the single layer natural tubulose chitterlings acellular matrix film of the invention produced is dry in conjunction with metal mesh
Photo figure.
Specific embodiment
Present invention firstly provides a kind of small intestine acellular matrix film combination metal mesh wall of the chest patching material preparation method, the party
Method concrete operations are as follows:
Step 1: taking the white pig of real estate 1, weight in 50-100Kg, using my seminar's invention " natural tubulose small intestine is de-
Cellular matrix film and its preparation method " preparation method produce the natural tubulose acellular matrix film of small intestine, then its stringer is splitted
Produce sheet acellular matrix film.
Step 2: different size of metal mesh is produced using titanium nickel alloy material, metal mesh has certain toughness and bullet
Property, it cannot change and generation activity with thoracic cavity internal and external pressure difference after being implanted into the wall of the chest.The Ti-Ni alloy wire that this experiment uses is straight
Diameter is respectively as follows: two kinds of specifications of 1.0mm and 0.8mm.First take the Ti-Ni alloy wire right-angled intersection of 2 diameters 1.0mm, long 10cm
And weld together crosspoint, one group is separately welded with method, the wire that 2 groups of crosses are welded will be round by the center of circle of pad
2 pads are welded together after average 8 equal parts, pound downhand welding contact, its thickness is made to be no more than 2mm, and by its central point to flat
It slightly protrudes the side in face.The Ti-Ni alloy wire for taking diameter 0.8mm long 50cm, is made into spiral shape by the center of circle of one end,
And keep all wires in one plane, adjacent two wires spacing 5.0mm.The wire of both the above specification is folded
Add, and weld the wire of overlapping, obtains center slightly to the arachnoid metal mesh of plane side protrusion.
Step 3: the small intestine acellular matrix film that step 1 obtains is fixed on the metal mesh that step 2 obtains, is obtained
Wall of the chest sticking patch.The metal mesh that step 2 is obtained first is fixed in particular stent, and the small intestine that step 1 is obtained takes off cell base
Plasma membrane is laid on the metal mesh that step 2 obtains, and winds repeatedly, so that acellular matrix film is overlapped layer 2-4, keeps certain
Tension, in order to avoid generate fold and by edge locked in the edge of metal mesh.
Step 4: the wall of the chest patching material that step 3 is obtained is through linear accelerating with 8000 ladd radiological dose radiation sterilizations
After be sealed.
According to the present invention, the small intestine that will be intercepted in animals is handled, and obtains small intestine acellular matrix film,
The animal is not particularly limited, preferably pig, ox, sheep, mouse or horse.
It is described to handle the wire of Ti-Ni alloy according to the present invention, the metal mesh of Ti-Ni alloy is obtained, it is described
Metal mesh is made of wire, preferably titanium nickel alloy material.
According to the present invention, producing for small intestine acellular matrix film is seminar invent that " natural tubulose small intestine takes off using me
Cellular matrix film and its preparation method " in preparation method produce out natural tubulose acellular matrix film after tubulose is taken off into cell again
Matrix membrane stringer is splitted, and sheet small intestine acellular matrix film is obtained.
According to the present invention, metal mesh is to use Ti-Ni alloy wire for preferred material.
According to the present invention, small intestine acellular matrix film combination metal mesh wall of the chest patching material obtained above is done
Dry, sealing, sterilizing save, spare.The intestines acellular matrix film combination metal mesh that the sterilizing will preferably be sealed produces chest
Wall patching material is through linear accelerating with 8000 ladd radiological dose radiation sterilizations.
It below for illustrating the present invention, but is not used in and limits the scope of the invention, unless otherwise specified, used in the present invention
The conventional means that are well known to those skilled in the art of technological means, raw materials used is commercial goods.
1. sheet ECM's produces
Take the white pig of real estate 1, weight in 50-100Kg, using my seminar's invention " natural tubulose small intestine takes off cell base
Plasma membrane and its preparation method " preparation method produce the natural tubulose acellular matrix film of small intestine, then its stringer is splitted and produces piece
Shape acellular matrix film.
2. Ti-Ni alloy metal mesh is produced
Ti-Ni alloy wire is taken, produces to obtain center slightly to the arachnoid metal mesh of plane side protrusion.
Fig. 1 is the photo figure of single layer chitterlings acellular matrix film of the present invention；Fig. 2 is de- for single layer sheet chitterlings of the present invention
Photo figure before cellular matrix film is dry in conjunction with metal mesh；
Fig. 3 is the photo figure after single layer sheet chitterlings acellular matrix film of the present invention is dry in conjunction with metal mesh；Fig. 2 is extremely
Fig. 3 explanation, acellular matrix film are tightly combined with metal mesh, successfully produce small intestine acellular matrix film combination metal mesh wall of the chest benefit
Piece.
Claims (7)
1. small intestine acellular matrix film combination metal mesh wall of the chest sticking patch preparation method, which is characterized in that this method comprises:
Step 1: acellular matrix film is produced with the small intestine intercepted in animals；
Step 2: producing different size of metal mesh using titanium nickel alloy material, and metal mesh has certain toughness and elasticity, plants
It cannot change and generation activity with thoracic cavity internal and external pressure difference after entering the wall of the chest；
Step 3: the small intestine acellular matrix film that step 1 obtains is fixed on the metal mesh that step 2 obtains, the wall of the chest is obtained
Patching material；
Step 4: the wall of the chest patching material that step 3 is obtained is close after linear accelerating is with 8000 ladd radiological dose radiation sterilizations
Envelope saves.
2. a kind of small intestine acellular matrix film combination metal mesh wall of the chest sticking patch preparation method according to claim 1, special
Sign is that the animal of the step one is pig, ox, sheep, mouse, horse, and metal material is titanium nickel alloy material.
3. a kind of small intestine acellular matrix film combination metal mesh wall of the chest sticking patch preparation method according to claim 1, special
Sign is that the acellular matrix film in the step one is the acellular matrix film that the small intestine of living animal is produced.
4. a kind of small intestine acellular matrix film combination metal mesh wall of the chest sticking patch preparation method according to claim 1, special
Sign is that the metal material of the step two is titanium nickel alloy material, and side reaction caused by the material is few, has been widely applied
Reparation in human body or the fixation device as fracture applied to human body should, put centered on metal mesh and slightly protruded to plane side
Web-like structure, the structural stability is more preferable, is unlikely to deform, and bigger tension can be resisted with less material, reduces metal object
The influence of confrontation human body.
5. a kind of small intestine acellular matrix film combination metal mesh wall of the chest sticking patch preparation method according to claim 1, special
Sign is, the combination of step three the small intestine acellular matrix film and metal mesh specifically: be fixed on metal mesh specifically
On holder device, small intestine acellular matrix film is tiled along the diameter of circular metal net and is wound in the two sides of metal mesh, makes it
It can be closely linked with metal mesh.
6. a kind of small intestine acellular matrix film combination metal mesh wall of the chest sticking patch preparation method according to claim 1, special
Sign is, the sterilizing of the step four be the wall of the chest patching material that will be sealed through linear accelerating with 8000 ladd radiological doses
Radiation sterilization.
7. preparation method described in claim 1-6 any one is small intestine acellular matrix film combination metal mesh wall of the chest sticking patch
Preparation method.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810839040.3A CN108939159A (en) | 2018-07-27 | 2018-07-27 | Small intestine acellular matrix film combination metal mesh wall of the chest sticking patch preparation method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810839040.3A CN108939159A (en) | 2018-07-27 | 2018-07-27 | Small intestine acellular matrix film combination metal mesh wall of the chest sticking patch preparation method |
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| CN108939159A true CN108939159A (en) | 2018-12-07 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1656959A2 (en) * | 2004-11-12 | 2006-05-17 | DePuy Products, Inc. | System attaching soft tissue to an implant |
| CN101721262A (en) * | 2008-10-15 | 2010-06-09 | 周星 | Tissue engineering combined human body lumen succedaneum |
| CN102266585A (en) * | 2011-07-20 | 2011-12-07 | 中国人民解放军第三军医大学第一附属医院 | Biological composite patch for female pelvic floor and manufacturing method thereof |
| CN105311678A (en) * | 2015-11-24 | 2016-02-10 | 无锡中科光远生物材料有限公司 | Complex hernia patch and preparing method and application thereof |
| CN107693848A (en) * | 2017-10-22 | 2018-02-16 | 长沙秋点兵信息科技有限公司 | Biomedical metal material and preparation method thereof |
-
2018
- 2018-07-27 CN CN201810839040.3A patent/CN108939159A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1656959A2 (en) * | 2004-11-12 | 2006-05-17 | DePuy Products, Inc. | System attaching soft tissue to an implant |
| CN101721262A (en) * | 2008-10-15 | 2010-06-09 | 周星 | Tissue engineering combined human body lumen succedaneum |
| CN102266585A (en) * | 2011-07-20 | 2011-12-07 | 中国人民解放军第三军医大学第一附属医院 | Biological composite patch for female pelvic floor and manufacturing method thereof |
| CN105311678A (en) * | 2015-11-24 | 2016-02-10 | 无锡中科光远生物材料有限公司 | Complex hernia patch and preparing method and application thereof |
| CN107693848A (en) * | 2017-10-22 | 2018-02-16 | 长沙秋点兵信息科技有限公司 | Biomedical metal material and preparation method thereof |
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Application publication date: 20181207 |
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