CN108938762A - A method of effective component in herbal medicine is found by chromatography separation, recombinant - Google Patents
A method of effective component in herbal medicine is found by chromatography separation, recombinant Download PDFInfo
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- CN108938762A CN108938762A CN201810910237.1A CN201810910237A CN108938762A CN 108938762 A CN108938762 A CN 108938762A CN 201810910237 A CN201810910237 A CN 201810910237A CN 108938762 A CN108938762 A CN 108938762A
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- 238000000034 method Methods 0.000 title claims abstract description 27
- 238000004587 chromatography analysis Methods 0.000 title claims abstract description 23
- 241000411851 herbal medicine Species 0.000 title claims abstract description 18
- 238000000926 separation method Methods 0.000 title claims description 11
- 150000001875 compounds Chemical class 0.000 claims abstract description 52
- 239000003814 drug Substances 0.000 claims abstract description 46
- 238000002474 experimental method Methods 0.000 claims abstract description 24
- 239000004615 ingredient Substances 0.000 claims abstract description 22
- 229940079593 drug Drugs 0.000 claims abstract description 8
- 241001465754 Metazoa Species 0.000 claims abstract description 7
- 239000000203 mixture Substances 0.000 claims abstract description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 5
- 201000010099 disease Diseases 0.000 claims abstract description 4
- 230000000813 microbial effect Effects 0.000 claims abstract description 3
- 230000008569 process Effects 0.000 claims abstract description 3
- 239000007788 liquid Substances 0.000 claims description 39
- 241000234314 Zingiber Species 0.000 claims description 18
- 235000006886 Zingiber officinale Nutrition 0.000 claims description 18
- 235000008397 ginger Nutrition 0.000 claims description 18
- 238000004128 high performance liquid chromatography Methods 0.000 claims description 14
- 238000004440 column chromatography Methods 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 230000000975 bioactive effect Effects 0.000 claims description 8
- 239000000126 substance Substances 0.000 claims description 8
- 238000002791 soaking Methods 0.000 claims description 7
- 208000003698 Heroin Dependence Diseases 0.000 claims description 6
- 206010013663 drug dependence Diseases 0.000 claims description 6
- VIKNJXKGJWUCNN-XGXHKTLJSA-N norethisterone Chemical compound O=C1CC[C@@H]2[C@H]3CC[C@](C)([C@](CC4)(O)C#C)[C@@H]4[C@@H]3CCC2=C1 VIKNJXKGJWUCNN-XGXHKTLJSA-N 0.000 claims description 6
- 241000712461 unidentified influenza virus Species 0.000 claims description 6
- 208000019206 urinary tract infection Diseases 0.000 claims description 5
- 238000004458 analytical method Methods 0.000 claims description 4
- DTGKSKDOIYIVQL-WEDXCCLWSA-N (+)-borneol Chemical compound C1C[C@@]2(C)[C@@H](O)C[C@@H]1C2(C)C DTGKSKDOIYIVQL-WEDXCCLWSA-N 0.000 claims description 3
- REPVLJRCJUVQFA-UHFFFAOYSA-N (-)-isopinocampheol Natural products C1C(O)C(C)C2C(C)(C)C1C2 REPVLJRCJUVQFA-UHFFFAOYSA-N 0.000 claims description 3
- 241000209495 Acorus Species 0.000 claims description 3
- 241000255791 Bombyx Species 0.000 claims description 3
- 241001148782 Davallia Species 0.000 claims description 3
- 241000205407 Polygonum Species 0.000 claims description 3
- 241000155589 Pteroxygonum Species 0.000 claims description 3
- 240000001341 Reynoutria japonica Species 0.000 claims description 3
- 235000018167 Reynoutria japonica Nutrition 0.000 claims description 3
- 229940116229 borneol Drugs 0.000 claims description 3
- CKDOCTFBFTVPSN-UHFFFAOYSA-N borneol Natural products C1CC2(C)C(C)CC1C2(C)C CKDOCTFBFTVPSN-UHFFFAOYSA-N 0.000 claims description 3
- DTGKSKDOIYIVQL-UHFFFAOYSA-N dl-isoborneol Natural products C1CC2(C)C(O)CC1C2(C)C DTGKSKDOIYIVQL-UHFFFAOYSA-N 0.000 claims description 3
- 230000006978 adaptation Effects 0.000 claims description 2
- 230000008901 benefit Effects 0.000 abstract description 3
- 230000007812 deficiency Effects 0.000 abstract description 3
- 230000000694 effects Effects 0.000 description 8
- BQJCRHHNABKAKU-KBQPJGBKSA-N morphine Chemical compound O([C@H]1[C@H](C=C[C@H]23)O)C4=C5[C@@]12CCN(C)[C@@H]3CC5=CC=C4O BQJCRHHNABKAKU-KBQPJGBKSA-N 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 238000001514 detection method Methods 0.000 description 4
- 238000001294 liquid chromatography-tandem mass spectrometry Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 3
- 239000002552 dosage form Substances 0.000 description 3
- 229960005181 morphine Drugs 0.000 description 3
- 239000003381 stabilizer Substances 0.000 description 3
- 238000010189 synthetic method Methods 0.000 description 3
- 229940088594 vitamin Drugs 0.000 description 3
- 229930003231 vitamin Natural products 0.000 description 3
- 235000013343 vitamin Nutrition 0.000 description 3
- 239000011782 vitamin Substances 0.000 description 3
- 150000003722 vitamin derivatives Chemical class 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 2
- 229940123424 Neuraminidase inhibitor Drugs 0.000 description 2
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000002911 sialidase inhibitor Substances 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 240000007185 Albizia julibrissin Species 0.000 description 1
- 235000011468 Albizia julibrissin Nutrition 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 125000001271 cephalosporin group Chemical group 0.000 description 1
- 238000009509 drug development Methods 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000004895 liquid chromatography mass spectrometry Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- PGZUMBJQJWIWGJ-ONAKXNSWSA-N oseltamivir phosphate Chemical group OP(O)(O)=O.CCOC(=O)C1=C[C@@H](OC(CC)CC)[C@H](NC(C)=O)[C@@H](N)C1 PGZUMBJQJWIWGJ-ONAKXNSWSA-N 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000010188 recombinant method Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 210000001635 urinary tract Anatomy 0.000 description 1
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
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Abstract
It is separated the invention discloses a kind of by chromatography, the method that recombinant finds effective component in herbal medicine, according to the experience or report to attend in the past, Chinese traditional medicine composition of the selection for certain treatment diseases, extract their compound, they are separated with chromatography, again combine them, concentration, select the animal adapted to or Microbial Model, with different compound combinations, do animal or Microbiology Experiment, remove ineffective ingredient in an experiment, this process repeatedly, until ineffective ingredient cannot be removed again, various forms of drugs are made in the compound that will be seen that, compound and other compounds for treating patient or will be seen that are mixed into various forms of drugs, for treating patient.Method that this is separated by chromatography, recombinant finds effective component in herbal medicine, this method contains all advantages of Chinese medicine, and overcomes the deficiency of their some key, at the same also overcome hair Western medicine develop in time long, costly disadvantage.
Description
Technical field
The invention belongs to traditional Chinese medicine research technical fields, find grass by chromatography separation, recombinant more particularly to a kind of
The method of effective component in medicine.
Background technique
There are many method of Study of Traditional Chinese Medicine ingredient, such as high pressure liquid chromatography (HPLC), compounds GC-MS (GC-MS), liquid-matter connection
With (LC-MS), liquid-matter-matter combination (LC-MS-MS), finger-print etc..But these methods can only determine a certain peak correspondingization
It studies point and structure (if known to them), these ingredient and validity can not be allowed clearly to connect.Therefore, how is Chinese medicine
Generate the drug effect always scientific bottleneck of the puzzlement modernization of Chinese medicine, Chinese medicine.
One secondary Chinese medicine may have tens kinds of ingredients, and the overwhelming majority is unrelated with therapeutic effect in these ingredients.If can remove
Its invalid components several greatly absolutely is only left effective or possible effective ingredient, is possible to completely control as Western medicine in this way
The quality and content being made point.
For current drug development research complex steps, it is unable to control the problems such as quality, the present invention provides a kind of chromatography
The method for analyzing potential effective component in Rapid identification Chinese medicine.
Summary of the invention
The object of the present invention is to provide it is a kind of by chromatography separation, recombinant find herbal medicine in effective component method,
To make up the deficiencies in the prior art.
To solve the above problems, the technical solution adopted by the present invention are as follows: a kind of to find grass by chromatography separation, recombinant
The method of effective component in medicine, comprising the following steps:
The experience or report that S1, basis attended in the past, selection extract it for the Chinese traditional medicine compositions of certain treatment diseases
Compound；
S2, they are separated with chromatography, then combines them, be concentrated；
S3, the animal of selection adaptation or Microbial Model do animal or Microbiology Experiment with different compound combinations；
S4, remove ineffective ingredient in an experiment, repeatedly this process, until cannot remove again it is ineffective at
Point；
Various forms of drugs are made in S5, the compound that will be seen that, for treating patient；
S6, the compound that will be seen that and other compounds are mixed into various forms of drugs, for treating patient.
Preferably, including in chromatography discovery Chinese medicine there is treatment heroin addiction bioactive compound, wherein treating
Heroin addiction Chinese medicine is prepared from the following raw materials in parts by weight: 140 grams of Radix Salviae Miltiorrhizae, 90 grams of acorus graminens soland, and 30 grams of the rhizome of davallia, silk tree
30 grams of skin, 20 grams of the fleece-flower root, 3.5 grams of borneol, comprising the following steps:
S1, the mixing of Six-element herbal medicine is blended, in addition the water of three times, soaking 30 minutes, boils at 100 degree and stroke 60 minutes, will receive
The liquid of collection, is repeated, and is boiled the liquid of collection at 100 degrees Celsius and is stroked to 20-30 milli；
S2, with HPLC analysis collect liquid, altogether at ten parts, when whole liquid together when, discovery have 61 ingredients, general
Ten parts of the liquid obtained with column chromatography are grouped, by each group of constitution number.
Preferably, a method of effective component in herbal medicine is found by chromatography separation, recombinant, including is chromatographed with column
Method finds to treat urinary tract infections bioactive compound in Chinese medicine, wherein treatment urinary tract infections Chinese medicine, by the original of following parts by weight
Material medicine is made: 30 grams of polygonum cuspidate, 30 grams of Girald Pteroxygonum Root, 20 grams of RHIZOMA COPTIDIS from Sichuan of China, 20 grams of CORTEX PHELLODENDRI CHINENSE, comprising the following steps:
S1, the mixing of 4 taste herbal medicine is blended, in addition the water of three times, soaking 30 minutes, boils at 100 degree and stroke 60 minutes, will collect
Liquid, repeat, the liquid of collection boiled at 100 degrees Celsius and is stroked to 20-30 milli；
S2, analyzed with HPLC, when whole liquid together when, discovery has 88 ingredients, and their content can be obtained, will
It is divided into ten portions with the liquid that column chromatography obtains, and by each composition lacing.Preferably, including with column chromatography ginger is found
Influenza virus bioactive compound is treated in dissipating, wherein treatment influenza virus Chinese medicine, is mainly dissipated by ginger and formed, wherein ginger
Scattered ingredient is beautiful, rhizome and bombyx batryticatus, and the scattered optimum concentration of ginger is 1000 μ g/ml, comprising the following steps:
S1, it blends ginger and dissipates, in addition the water of three times, soaking 30 minutes, boils at 100 degree and strokes 60 minutes, by the liquid of collection,
It repeats, the liquid of collection is boiled at 100 degrees Celsius and is stroked to 20-30 milli；
S2, with HPLC analysis ginger dissipate, when whole liquid together when, discovery have 42 chemical components, will be chromatographed with column
The liquid that method obtains is divided into seven parts, and by each group of constitution number.
Technical effect and advantage of the invention: the side of effective component in herbal medicine should be found by chromatography separation, recombinant
Method can be in Rapid identification Chinese medicine to the effective component of certain disease, and by proposing effective component by the method with chromatography
Take, purify, be enriched with after be made into tablet, work as health care product, treat patient, this method contains all advantages of Chinese medicine, and gram
The deficiency of their some keys has been taken, while also having overcome time long, costly disadvantage in hair Western medicine exploitation, provides one for patient
The scheme that kind is quickly treated.
Detailed description of the invention
Fig. 1 is the comparison of Chinese medicine and Western medicine of the present invention；
Fig. 2 is the comparison of Chinese medicine, Western medicine and " third selection " of the present invention；
Fig. 3 is the flow chart that the present invention determines possible effective component in Chinese medicine with chromatography.
Specific embodiment
The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments:
Embodiment 1
It is reported that treatment heroin addiction Chinese medicine is made of Six-element herbal medicine: 140 grams of Radix Salviae Miltiorrhizae, 90 grams of acorus graminens soland, the rhizome of davallia
30 grams, 30 grams of cortex albiziae, 20 grams of the fleece-flower root, 3.5 grams of borneol.
S1, the mixing of Six-element herbal medicine is blended, in addition the water of three times, soaking 30 minutes, boils at 100 degree and stroke 60 minutes, will receive
The liquid of collection, is repeated.The liquid of collection is boiled at 100 degrees Celsius and is stroked to 20-30 milliliters；
S2, the liquid collected with HPLC analysis, altogether at ten parts.When whole liquid together when, discovery have 61 ingredients.It will
It is with ten parts of groupings of the liquid that column chromatography obtains, each group of constitution number is as follows:
First group of experiment: it is tested with whole components
The source of rat and preparation are all identical as the article of Yao-ChangChiang.It is fed with 0.1 mg/kg of morphine
It supports 15 days.
In the liquid that HPLC analysis is collected, No. 10 peak of discovery is obviously stablized, with it for quantitative standard, by collection
No. 10 substances in liquid are diluted to 10 mg/mls.
With six groups of experiments, first to fourth group is the ingredient (10mgpeak10/ put in mouse cage by chromatography
Ml) and the bottle of distilled water, the 5th group is the bottle that morphine (0.1mg/ml) and distilled water is put in mouse cage, and the 6th group is
The bottle of physiological saline and distilled water is put in mouse cage.
Isolated compound is carried out to different combinations, then mouse is placed in mouse cage, them is observed and inhales different bottles
Number, as a result as follows:
It is if animal reaches the 90% of morphine group containing the number of compound bottle in first to fourth group of suction, then good for effect
It is good.
Second group of experiment: it is tested with fractions
From in above-mentioned experiment it can be seen that active principle may be in the 2nd and the 9th part.Again by the 2nd and the 9th group of chemical combination
Object is grouped again, carries out experiment below, as a result as follows:
1, effect definition with it is upper identical.
From experiment 2, it can be seen that possible active principle is 9,10,51, No. 52 compounds, people can continue to do look at be
It is no to remove inert matter therein.Database detection or LC-MS-MS analysis are carried out to 4 kinds of compounds, find 9, No. 51 compounds
Can be commercially available, 10, No. 52 compounds cannot be commercially available, but can obtain industrial dosage by purifying or synthetic method.
9,10,51, No. 52 compounds are mixed in proportion, is mixed with stabilizer or vitamin, different dosage forms is made, be used for
Treat heroin addiction disease.
Embodiment 2 is found to treat urinary tract infections bioactive compound in Chinese medicine with column chromatography
Doctor treats various bacteria infection, patient totally two ten people with Chinese medicine.Patient is participated in not in the case where double blind
Same treatment group.Therapeutic effect is good.These traditional Chinese medicine ingredients are: 30 grams of polygonum cuspidate, 30 grams of Girald Pteroxygonum Root, and 20 grams of RHIZOMA COPTIDIS from Sichuan of China, CORTEX PHELLODENDRI CHINENSE
20 grams.Preparation method is as described in the text.
Analyzed with HPLC, when whole liquid together when, discovery have 88 ingredients.And their content can be obtained.It will use
The liquid that column chromatography obtains is divided into ten portions, and each group of constitution number is as follows:
First group of experiment: it is tested with whole components, determines effective concentration.
In the liquid that HPLC analysis is collected, No. 20 peak of discovery is obviously stablized, with it for quantitative standard, by collection
10 substances in liquid are diluted to 50 mg/mls.With the method for conventional detection drug sensitivity test, it is added in Agar solution
1,2,4 milliliter contains the above dilute solution, and last total amount is all 50 milliliters.Escherichia coli are applied to, 37 degree of guarantors are placed in
Incubator, find to be added 2 and 4 milliliters or more dilute solutions after 24 hours in solution has apparent bactericidal effect.
Second group of experiment: it is tested with whole components.
Above 88 compounds are divided into 4 groups, and add two groups of controls, then do bacterium sensitive experiment, as a result as follows:
1, such as experimental group reaches the 90% of cephalosporin group effect, as well.
2、5mg/50cc。
The experiment of third group: it is tested with fractions.
From testing above: 32 compounds in 1-4 group compound contain effective component, they are separated into 4
Group, and two groups of controls are added, it is as a result as follows:
4th group of experiment: it is tested with fractions.
Find out from the above experiment, so grouping effect is bad, the above 1-4 group compound is divided into 4 groups again, and add
Two groups of controls, as a result as follows:
5th group of experiment: it is tested with fractions.
From testing above: 8 compounds being grouped in 4 compounds again contain effective component, they are divided again
At 4 groups, and two groups of controls are added, as a result as follows:
From experiment 5, it can be seen that possible active principle is 17,18,27, No. 28 compounds, carries out data to 4 kinds of compounds
Library detection or LC-MS-MS analysis find that 17, No. 27 compounds can be commercially available, and 18, No. 28 compounds cannot be commercially available, but can lead to
It crosses purifying or synthetic method obtains industrial dosage.
Compound is mixed in proportion, is mixed with stabilizer or vitamin, different dosage forms are made, for treating in urinary tract
Thin Jun infection.
The column chromatography of embodiment 3 finds that ginger treats influenza virus bioactive compound in dissipating
2018 1 groups of Chinese Scientists have delivered article, be dispersed in ginger all obtained in various kinds of cell and zoopery it is good
The effect of good inhibition influenza virus.The scattered ingredient of ginger is beautiful, rhizome and bombyx batryticatus.Here, the inventor of this patent is to want
Determining wherein may compounds effective.
In article, author reports the work of neuraminidase inhibitor when ginger is scattered to up to the concentration of 1000 μ g/ml
Property reduces 80%.In secondary test, the present inventor finds out possible active compound with same method.
With HPLC analysis ginger dissipate, when whole liquid together when, discovery have 42 chemical components.Their content can also
It analyzes to obtain by HPLC.
The liquid obtained with column chromatography is divided into seven parts, each group of constitution number is as follows:
First group of experiment: being tested with whole components, determines the effect that neuraminidase inhibitor is dissipated with ginger.
By the above compound, it is divided into 4 groups, and adds two groups of controls, as a result as follows:
1, such as experimental group reaches the 90% of Tamiflu group effect, as well.
2、5mg/50cc。
Second group of experiment: it is tested with fractions
By the compound of the above intermediate 3-5 group, it is divided into 3 groups, and adds two groups of controls, as a result as follows:
The experiment of third group: it is tested with fractions
By the 3rd group of compound in being grouped again above, 3 groups are separated into, and add two groups of controls, as a result as follows:
It can be seen that possible active principle is 15,17, No. 18 compounds from experiment three, data are carried out to 3 kinds of compounds
Library detection or LC-MS-MS analysis, 17, No. 27 compounds of discovery are it is known that simultaneously can be commercially available, and 18, No. 28 compounds are unable to quotient
Purchase, but industrial dosage can be obtained by purifying or synthetic method.
15,17, No. 18 compounds are mixed in proportion, is mixed with stabilizer or vitamin, different dosage forms is made, for controlling
It treats.It can be also used for discovery with same method and treat its microbiological Chinese traditional medicine (herbal medicine) ingredient.
Finally, it should be noted that the foregoing is only a preferred embodiment of the present invention, it is not intended to restrict the invention,
Although the present invention is described in detail referring to the foregoing embodiments, for those skilled in the art, still may be used
To modify the technical solutions described in the foregoing embodiments or equivalent replacement of some of the technical features,
All within the spirits and principles of the present invention, any modification, equivalent replacement, improvement and so on should be included in of the invention
Within protection scope.
Claims (4)
1. a kind of method for finding effective component in herbal medicine by chromatography separation, recombinant, which is characterized in that including following step
It is rapid:
The experience or report that S1, basis attended in the past, selection extract theirs for the Chinese traditional medicine compositions of certain treatment diseases
Compound；
S2, they are separated with chromatography, then combined them, concentration；
S3, the animal of selection adaptation or Microbial Model do animal or Microbiology Experiment with different compound combinations；
S4, remove ineffective ingredient in an experiment, repeatedly this process, until ineffective ingredient cannot be removed again；
Various forms of drugs are made in S5, the compound that will be seen that, for treating patient；
S6, the compound that will be seen that and other compounds are mixed into various forms of drugs, for treating patient.
2. a kind of method for finding effective component in herbal medicine by chromatography separation, recombinant according to claim 1, packet
Including has treatment heroin addiction bioactive compound in chromatography discovery Chinese medicine, wherein treatment heroin addiction Chinese medicine is by such as
The bulk pharmaceutical chemicals of lower parts by weight are made: 140 grams of Radix Salviae Miltiorrhizae, 90 grams of acorus graminens soland, and 30 grams of the rhizome of davallia, 30 grams of cortex albiziae, 20 grams of the fleece-flower root,
3.5 grams of borneol, which comprises the following steps:
S1, the mixing of Six-element herbal medicine is blended, in addition the water of three times, soaking 30 minutes, boils at 100 degree and stroke 60 minutes, by collection
Liquid is repeated, and is boiled the liquid of collection at 100 degrees Celsius and is stroked to 20-30 milli；
S2, with HPLC analysis collect liquid, altogether at ten parts, when whole liquid together when, find have 61 ingredients, column will be used
Ten parts of groupings of the liquid that chromatography obtains, by each group of constitution number.
3. a kind of method for finding effective component in herbal medicine by chromatography separation, recombinant according to claim 1, packet
It includes and treats urinary tract infections bioactive compound in column chromatography discovery Chinese medicine, wherein treatment urinary tract infections Chinese medicine, by as follows
The bulk pharmaceutical chemicals of parts by weight are made: 30 grams of polygonum cuspidate, 30 grams of Girald Pteroxygonum Root, 20 grams of RHIZOMA COPTIDIS from Sichuan of China, 20 grams of CORTEX PHELLODENDRI CHINENSE, which is characterized in that including
Following steps:
S1, the mixing of 4 taste herbal medicine is blended, in addition the water of three times, soaking 30 minutes, boils at 100 degree and stroke 60 minutes, by the liquid of collection
Body is repeated, and is boiled the liquid of collection at 100 degrees Celsius and is stroked to 20-30 milli；
S2, analyzed with HPLC, when whole liquid together when, discovery has 88 ingredients, and obtains their content, will use column layer
The liquid that analysis method obtains is divided into ten portions, and by each group of constitution number.
4. a kind of method for finding effective component in herbal medicine by chromatography separation, recombinant according to claim 1, packet
It includes and dissipates middle treatment influenza virus bioactive compound with column chromatography discovery ginger, wherein treatment influenza virus Chinese medicine, mainly
Dissipated and formed by ginger, wherein the scattered ingredient of ginger be beautiful, rhizome and bombyx batryticatus, and the scattered optimum concentration of ginger is 1000 μ g/ml,
Characterized by comprising the following steps:
S1, blend ginger dissipate, in addition the water of three times, soaking 30 minutes, boils at 100 degree and strokes 60 minutes, by the liquid of collection, then weigh
It is one time multiple, the liquid of collection is boiled at 100 degrees Celsius and is stroked to 20-30 milli；
S2, with HPLC analysis ginger dissipate, when whole liquid together when, discovery have 42 chemical components, will be obtained with column chromatography
To liquid be divided into seven parts, and by each group of constitution number.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101129557A (en) * | 2007-07-31 | 2008-02-27 | 雷超清 | Traditional Chinese medicine for treating bacterial infection |
| CN104337882A (en) * | 2013-07-23 | 2015-02-11 | 刘永乐 | Traditional Chinese medicine composition for toxin expelling and drug rehabilitation |
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2018
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101129557A (en) * | 2007-07-31 | 2008-02-27 | 雷超清 | Traditional Chinese medicine for treating bacterial infection |
| CN104337882A (en) * | 2013-07-23 | 2015-02-11 | 刘永乐 | Traditional Chinese medicine composition for toxin expelling and drug rehabilitation |
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