CN108936633A - A kind of sheep placenta-red date fermentation nutrient solution and preparation method thereof - Google Patents

A kind of sheep placenta-red date fermentation nutrient solution and preparation method thereof Download PDF

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Publication number
CN108936633A
CN108936633A CN201810900420.3A CN201810900420A CN108936633A CN 108936633 A CN108936633 A CN 108936633A CN 201810900420 A CN201810900420 A CN 201810900420A CN 108936633 A CN108936633 A CN 108936633A
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China
Prior art keywords
sheep placenta
red date
fermentation
jujube
liquid
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Pending
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CN201810900420.3A
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Chinese (zh)
Inventor
杨海军
李谦
杨春梅
李君�
任焱
杨海兵
杨丹妮
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Ningxia Ningyang Food Co Ltd
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Ningxia Ningyang Food Co Ltd
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Priority to CN201810900420.3A priority Critical patent/CN108936633A/en
Publication of CN108936633A publication Critical patent/CN108936633A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof

Abstract

The present invention provides a kind of sheep placenta-red date fermentation nutrient solutions and preparation method thereof, jujube is starched first and is fermented by active dry yeast, make the part glucide experience initial fermentation process in jujube slurry, substantially reduces subsequent sheep placenta-jujube mixed liquor fermentation period, improve production efficiency;Secondly, it is fermented using the composite bacteria that 1.01 acetic acid bacterias form is made by acetobacter schuenhach and Shanghai to red date fermentation level-one liquid and primary sheep placenta liquid, effectively eliminate bitter taste and puckery mouthfeel in sheep placenta, by providing suitable growing environment for composite bacteria, to obtain higher product yield, a variety of natural active matters in sheep placenta and jujube are contained in product, and product is weakly acidic, is more advantageous to absorption of human body;In addition, the present invention makes full use of Ningxia, China sheep placenta abundant, jujube resource, high value-added product is developed, it is with good economic efficiency.

Description

A kind of sheep placenta-red date fermentation nutrient solution and preparation method thereof
Technical field
The invention belongs to technical field of health care food, and in particular to one kind is with sheep placenta-red date fermentation nutrient solution and its system Preparation Method.
Background technique
Sheep placenta just refers to the active material in sheep embryo or placenta (being referred to as sheep placenta), and the active position in sheep placenta is main For micromolecule polypeptide substance.Sheep placenta ingredient is complex, and main chemical compositions are immunoglobulin, a variety of hormones such as rush property Glandular hormone, prolactin, thyrotropic hormone, oxytocins substance, a variety of steroid hormones;Also containing the enzyme for having a variety of application values and Interferon;For its protein content 80% or more, amino acid classes are complete, and proportion is reasonable;In addition there are multivitamins and micro Element.From the point of view of modern medicine, placenta can generate a variety of steroid hormones, adjust peptide, growth factor, cell factor, they The Effect of Mutual Regulation extremely loaded is generated in placenta, to maintain the growth and development of placenta, and enters blood circulation, it is raw to fetus The endocrine function of long development and parent plays important adjustment effect.Amino acid rich in sheep placenta, polypeptide and other Bioactive substance can significantly increase human body immune function, enriching yin and nourishing kidney, nourishing qi and blood, anti-aging.
Jujube in existing more than 4,000 years plantation history of China, be just listed in since ancient times " five fruits " (peach, Lee, plum, apricot, One of jujube), containing inhibition cancer cell, or even substance of the cancer cell to normal cell turnover can be made.Jujube warm-natured, returns spleen stomach sweet in flavor There are tonifying middle-Jiao and Qi, nourishing blood and tranquilization, the function of mitigating pharmacological property;And modern pharmacology then finds that jujube contains protein, rouge The nutritions abundant such as a variety of amino acid of fat, carbohydrate, organic acid, vitamin A, vitamin C, micro-calcium.
Chinese patent CN201110193938.6 discloses a kind of sheep embryo and sheep placenta biological product and preparation method thereof, This method includes the following steps: 1) sheep embryo and Goat Placenta raw material pre-treatment;2) sheep embryo and Goat Placenta ultramicro grinding, cell Broken wall;3) extraction of sheep embryo and Goat Placenta and enzymatic hydrolysis;4) using sephadex according to molecular size range to above-mentioned steps 3) Obtained in sheep placenta and sheep placenta stoste carry out Grade refining, obtain amino acid, micromolecule polypeptide and various molecular weight proteins Refining sheep placenta element.Using the element of the refining sheep placenta of sheep embryo and sheep placenta made from the above method, both retain the day in placenta Right active material, and increase its polypeptide and amino acid content.Although preparing the common skill that sheep placenta belongs to this field by enzymatic hydrolysis Art means, but in the technical process such as sheep embryo and Goat Placenta ultramicro grinding, breaking-wall cell and enzymatic hydrolysis, it is easy to in sheep placenta Active material damages, and loses the utility value of sheep placenta, and enzymatic isolation method higher cost, gained protein product purity is not It is high.
Summary of the invention
The object of the present invention is to provide a kind of sheep placenta-red date fermentation nutrient solutions and preparation method thereof, respectively with sheep placenta and Red date as raw materials are extracted the effective component in sheep placenta and jujube by microorganism fermentation process, are effectively eliminated in Goat Placenta Bitter taste and puckery mouthfeel, the nutritional ingredient in products obtained therefrom is compared with horn of plenty, and products obtained therefrom yield is high.
Sheep placenta of the present invention-red date fermentation nutrient solution preparation method, includes the following steps:
Step 1: the silt and impurity on jujube surface are rinsed with the clear water of flowing, jujube is enucleated after taking-up and uses mashing Jujube meat is broken into jujube slurry by machine, the primary sterilizing jujube slurry after jujube slurry to be heated to cook to sterilizing;
Step 2: the inoculating active dry ferment in sterilizing jujube slurry ferments, fermentation starts to examine after 7 days in the fermenter The residual sugar amount in fermentation liquid is surveyed, terminates fermentation after residual sugar amount is lower than 5%, obtains red date fermentation level-one liquid;
Step 3: fresh sheep placenta is taken, is crushed under the conditions of 0-5 DEG C using meat grinder after removal of impurities, cleaning, homogenate, then through going out Primary sheep placenta liquid is obtained after bacterium process;
Step 4: red date fermentation level-one liquid is mixed in apparatus for acetic acid fermentation tank with primary sheep placenta liquid, is inoculated with by Xu Shi acetic acid The composite bacteria of 1.01 acetic acid bacterias composition is made in bacillus and Shanghai, obtains sheep placenta-red date fermentation second level liquid after the 120-180h that ferments;
Step 5: sheep placenta-red date fermentation second level liquid is concentrated by evaporation by ultrafilter filtering, rotary evaporator, to concentration Liquid, which sterilize, filling can obtain sheep placenta-red date fermentation nutrient solution.
Further, the operation temperature that jujube slurry is heated to cook sterilization process in step 1 is 95-98 DEG C, is sterilized the duration For 10-15min.
Further, step 2 mass fraction of inoculating active dry ferment in sterilizing jujube slurry is 0.4-0.6%, fermentation temperature Degree is 38-42 DEG C.
Further, the operation temperature of lamb embryo powder pulping liquid sterilization process is 115-120 DEG C in step 3, when sterilizing continues Between be 20-30min.
Further, step 4 mass fraction of inoculation composite bacteria in red date fermentation level-one liquid and primary sheep placenta liquid is 0.5-0.8%, fermentation temperature are 25-30 DEG C.
Further, the ratio that 1.01 acetic acid bacterias are made in acetobacter schuenhach and Shanghai in step 4 composite bacteria is 1:(0.8- 1.5)。
Further, step 5 carries out sheep placenta-red date fermentation second level liquid in the process of ultrafiltration, the filtering of the ultrafilter Precision is 0.35 μm.
It is fermented it is an advantage of the current invention that (1) starches jujube by active dry yeast, makes the part carbohydrate object in jujube slurry Matter undergoes primary fermentation process, and subsequent sheep placenta-jujube mixed liquor fermentation period is foreshortened to 120-180h by this technique, improves Production efficiency;(2) in step 2, the residual sugar amount measured in fermentation jujube slurry terminates to ferment lower than 5%, the residual sugar in jujube slurry It is respectively to make the composite bacteria that 1.01 acetic acid bacterias form by acetobacter schuenhach and Shanghai in step 4 to mention with the protein in sheep placenta For the carbon source and nitrogen source needed for fermenting, suitable growing environment is created for zymophyte, to obtain higher yield;(3) this hair It is bright to use the composite bacteria being made of acetobacter schuenhach and Shanghai 1.01 acetic acid bacterias of wine to red date fermentation level-one liquid and primary sheep placenta Liquid ferments, and effectively eliminates the bitter taste in sheep placenta and puckery mouthfeel, and products obtained therefrom high income is avoided using at enzymatic isolation method Sheep placenta is managed, the active principles such as polypeptide in sheep placenta are damaged, in addition, a variety of natural active matters are also rich in product, and Product is weakly acidic, is more advantageous to the absorption of human body;(4) present invention makes full use of Ningxia, China sheep placenta abundant, jujube resource, High value-added product is developed, it is with good economic efficiency.
Specific embodiment
In the following, embodiments of the present invention will be described.
Embodiment one:
Jujube, is enucleated and will be red with beater by the silt and impurity that jujube surface is rinsed with the clear water of flowing after taking-up Jujube meat breaks into jujube slurry, and jujube slurry is heated to cook after sterilizing 15min at 95 DEG C and obtains primary sterilizing jujube slurry;
The inoculating active dry ferment in sterilizing jujube slurry, the mass fraction of inoculating active dry ferment are 0.5%, in the fermenter It ferments, fermentation temperature is 38 DEG C, and fermentation started to detect the residual sugar amount in fermentation liquid after 7 days, is tied after residual sugar amount is lower than 5% Beam fermentation, obtains red date fermentation level-one liquid;
Fresh sheep placenta is taken, is crushed under the conditions of 0-5 DEG C using meat grinder after removal of impurities, cleaning, homogenate, then through going out at 120 DEG C Primary sheep placenta liquid is obtained after bacterium 20min;
Red date fermentation level-one liquid is mixed in apparatus for acetic acid fermentation tank with primary sheep placenta liquid, is inoculated with by acetobacter schuenhach and Shanghai The composite bacteria for making 1.01 acetic acid bacterias proportionally and be 1:0.86 composition, the mass fraction for being inoculated with composite bacteria is 0.55%, Sheep placenta-red date fermentation second level liquid is obtained after fermentation 120-180h at 25 DEG C;
Sheep placenta-red date fermentation second level liquid is concentrated by evaporation by precision for 0.35 μm of ultrafilter filtering, rotary evaporator, it is right Concentrate, which sterilize, filling can be obtained sheep placenta-red date fermentation nutrient solution.
The residual solids substance that ultrafiltration machine filter obtains is dried, using in Kjeldahl nitrogen determination residual solids substance Nitrogen content, by three groups of parallel tests, the N average content measured in residual solids substance is lower than 0.43%, it was demonstrated that uses this hair Bright institute's providing method fermentation sheep placenta, yield with higher.
Embodiment two:
Jujube, is enucleated and will be red with beater by the silt and impurity that jujube surface is rinsed with the clear water of flowing after taking-up Jujube meat breaks into jujube slurry, and jujube slurry is heated to cook after sterilizing 10min at 98 DEG C and obtains primary sterilizing jujube slurry;
The inoculating active dry ferment in sterilizing jujube slurry, the mass fraction of inoculating active dry ferment are 0.6%, in the fermenter It ferments, fermentation temperature is 42 DEG C, and fermentation started to detect the residual sugar amount in fermentation liquid after 7 days, is tied after residual sugar amount is lower than 5% Beam fermentation, obtains red date fermentation level-one liquid;
Fresh sheep placenta is taken, is crushed under the conditions of 0-5 DEG C using meat grinder after removal of impurities, cleaning, homogenate, then through going out at 115 DEG C Primary sheep placenta liquid is obtained after bacterium 30min;
Red date fermentation level-one liquid is mixed in apparatus for acetic acid fermentation tank with primary sheep placenta liquid, is inoculated with by acetobacter schuenhach and Shanghai The composite bacteria for making 1.01 acetic acid bacterias proportionally and be 1:1.5 composition, the mass fraction for being inoculated with composite bacteria is 0.8%, 30 Sheep placenta-red date fermentation second level liquid is obtained after fermentation 120-180h at DEG C;
Sheep placenta-red date fermentation second level liquid is concentrated by evaporation by precision for 0.35 μm of ultrafilter filtering, rotary evaporator, it is right Concentrate sterilize it is filling after sheep placenta-red date fermentation nutrient solution can be obtained.
The residual solids substance that ultrafiltration machine filter obtains is dried, using in Kjeldahl nitrogen determination residual solids substance Nitrogen content, by three groups of parallel tests, the N average content measured in residual solids substance is lower than 0.39%, it was demonstrated that uses this hair Bright institute's providing method fermentation sheep placenta, yield with higher.

Claims (7)

1. a kind of sheep placenta-red date fermentation nutrient solution preparation method, which comprises the steps of:
Step 1: with flowing clear water rinse jujube surface silt and impurity, after taking-up by jujube be enucleated and with beater will Jujube meat breaks into jujube slurry, and jujube slurry is heated to cook after sterilizing and obtains primary sterilizing jujube slurry;
Step 2: the inoculating active dry ferment in sterilizing jujube slurry ferments in the fermenter, and fermentation starts detection hair after 7 days Residual sugar amount in zymotic fluid terminates fermentation after residual sugar amount is lower than 5%, obtains red date fermentation level-one liquid;
Step 3: fresh sheep placenta is taken, is crushed under the conditions of 0-5 DEG C using meat grinder after removal of impurities, cleaning, homogenate, then sterilized work Primary sheep placenta liquid is obtained after sequence;
Step 4: red date fermentation level-one liquid is mixed in apparatus for acetic acid fermentation tank with primary sheep placenta liquid, is inoculated with by acetobacter schuenhach The composite bacteria of 1.01 acetic acid bacterias composition is made with Shanghai, obtains sheep placenta-red date fermentation second level liquid after the 120-180h that ferments;
Step 5: by sheep placenta-red date fermentation second level liquid by ultrafilter filtering, rotary evaporator be concentrated by evaporation, to concentrate into Row sterilization is filling to can be obtained sheep placenta-red date fermentation nutrient solution.
2. sheep placenta as described in claim 1-red date fermentation nutrient solution preparation method, which is characterized in that the jujube slurry heating The operation temperature for simmering sterilization process is 95-98 DEG C, and the sterilizing duration is 10-15mi n.
3. sheep placenta as described in claim 1-red date fermentation nutrient solution preparation method, which is characterized in that the sterilizing jujube slurry The mass fraction of middle inoculating active dry ferment is 0.4-0.6%, and fermentation temperature is 38-42 DEG C.
4. sheep placenta as described in claim 1-red date fermentation nutrient solution preparation method, which is characterized in that the sheep placenta crushes The operation temperature of slurries sterilization process is 115-120 DEG C, and the sterilizing duration is 20-30mi n.
5. sheep placenta as described in claim 1-red date fermentation nutrient solution preparation method, which is characterized in that the red date fermentation The mass fraction that composite bacteria is inoculated in level-one liquid and primary sheep placenta liquid is 0.5-0.8%, and fermentation temperature is 25-30 DEG C.
6. sheep placenta as described in claim 1-red date fermentation nutrient solution preparation method, which is characterized in that the composite bacteria The ratio that 1.01 acetic acid bacterias are made in middle acetobacter schuenhach and Shanghai is 1:(0.8-1.5).
7. sheep placenta as described in claim 1-red date fermentation nutrient solution preparation method, which is characterized in that the ultrafilter Filtering accuracy is 0.35 μm.
CN201810900420.3A 2018-08-09 2018-08-09 A kind of sheep placenta-red date fermentation nutrient solution and preparation method thereof Pending CN108936633A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111820404A (en) * 2020-07-07 2020-10-27 肖启森 Sheep embryo extract beautifying oral liquid and preparation method thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1614000A (en) * 2004-07-15 2005-05-11 天津科技大学 Red date juice fermented vinegar and fermentation thereof
KR20110116885A (en) * 2010-04-20 2011-10-26 (주) 오엔씨 A preparation method of a fermented extract of horse placenta
CN106466329A (en) * 2015-08-20 2017-03-01 伊犁肖尔布拉克酒业有限责任公司 A kind of preparation method of Placenta caprae seu oviss fermented product
CN106880777A (en) * 2017-02-27 2017-06-23 武文海 A kind of Helth-care product with functions of prolonging life and nourishing face and preparation method thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1614000A (en) * 2004-07-15 2005-05-11 天津科技大学 Red date juice fermented vinegar and fermentation thereof
KR20110116885A (en) * 2010-04-20 2011-10-26 (주) 오엔씨 A preparation method of a fermented extract of horse placenta
CN106466329A (en) * 2015-08-20 2017-03-01 伊犁肖尔布拉克酒业有限责任公司 A kind of preparation method of Placenta caprae seu oviss fermented product
CN106880777A (en) * 2017-02-27 2017-06-23 武文海 A kind of Helth-care product with functions of prolonging life and nourishing face and preparation method thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111820404A (en) * 2020-07-07 2020-10-27 肖启森 Sheep embryo extract beautifying oral liquid and preparation method thereof

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Application publication date: 20181207