CN108926623A - Application of anemone extract in preparation of composition for improving SOD2 gene expression, repairing skin and preventing ultraviolet light damage - Google Patents
Application of anemone extract in preparation of composition for improving SOD2 gene expression, repairing skin and preventing ultraviolet light damage Download PDFInfo
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- CN108926623A CN108926623A CN201810494237.8A CN201810494237A CN108926623A CN 108926623 A CN108926623 A CN 108926623A CN 201810494237 A CN201810494237 A CN 201810494237A CN 108926623 A CN108926623 A CN 108926623A
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- pueraria lobota
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- 239000000284 extract Substances 0.000 title claims abstract description 45
- 239000000203 mixture Substances 0.000 title claims abstract description 13
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- 230000014509 gene expression Effects 0.000 title abstract description 3
- 230000008832 photodamage Effects 0.000 title abstract 2
- 238000002360 preparation method Methods 0.000 title description 4
- 241001083548 Anemone Species 0.000 title 1
- 241000219780 Pueraria Species 0.000 claims description 61
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/77—Sapindaceae (Soapberry family), e.g. lychee or soapberry
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/16—Emollients or protectives, e.g. against radiation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/004—Aftersun preparations
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Engineering & Computer Science (AREA)
- Dermatology (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Epidemiology (AREA)
- General Chemical & Material Sciences (AREA)
- Alternative & Traditional Medicine (AREA)
- Organic Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Medical Informatics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Toxicology (AREA)
- Birds (AREA)
- Medicines Containing Plant Substances (AREA)
- Cosmetics (AREA)
Abstract
The invention relates to application of a pachira angulatus extract, in particular to application of the pachira angulatus extract in preparing a composition for improving SOD2 gene expression, repairing skin and preventing ultraviolet light damage. The extract of Pueraria thomsonii has effects of preventing or treating ultraviolet light injury to skin.
Description
Technical field
The present invention relates to the purposes of a wind ship Pueraria lobota extract, are used to prepare promotion especially with respect to wind ship Pueraria lobota extract
The performance of SOD2 gene promotes skin repair and prevents skin by the purposes of the composition of ultraviolet light harms.
Background technique
Ultraviolet light (Ultraviolet, abbreviation UV) is a kind of electromagnetic wave and is present among sunlight that wavelength is
10nm (30PHz) to 400nm (750THz).Ultraviolet light is one kind of nocuity light, is caused via the absorption and meeting DNA of skin
Injury, when DNA sustains an injury cell can thus it is dead or develop into out of contior cancer cell, here it is tumor formed just
Phase.Ultraviolet light has been determined related with the generation of many diseases；Such as：Wrinkle, sunburn, cataract, cutaneum carcinoma, visual impairment with
The injury of immune system.
Ultraviolet light pass through atmosphere after be left main ultraviolet light can be sub-divided into according to its wavelength UVA (wavelength 320 to
400nm) and UVB (wavelength 280 to 320nm).UVA mainly will cause the deterioration of freckle and blackspot, and cause skin tanning,
It is the accomplice for causing skin aging.Though UVB will cause skin damage and aging, excessive exposure, and may cause acute sunburn
And the generation of chronic skin cancer, but the penetration power of UVA is strong, and general transparent glass can not all block penetrating for UVA, be daily life
The ultraviolet light that exposure is arrived is easiest in work.
It therefore, is the injury that can not prevent ultraviolet light completely in our daily lifes, even indoors, such as drive
Or window side activity indoors, it can all touch the exposure of UVA.Even if diligent smear sun-screening agent, sun-screening agent is also only to extend skin
The time that skin comes to harm.
Summary of the invention
To solve foregoing problems, the present invention, which provides a kind of wind ship Pueraria lobota extract and is used to prepare, to be promoted the performance of SOD2 gene, promotees
Into skin repair and prevention skin by the purposes of the composition of ultraviolet light harms, wherein the wind ship Pueraria lobota extract be wind ship Pueraria lobota with
The solvent of water, alcohol or aqueous alcohols is extracted and is obtained.
In one embodiment of this invention, which is the fruit of wind ship Pueraria lobota with water, alcohol or aqueous alcohols
Solvent is extracted and is obtained.
In one embodiment of this invention, a pharmaceutically acceptable carrier is further included, and the composition can be
Powder, particle, liquid, glue or dosage form paste.
In one embodiment of the invention, the volume ratio of the solvent and wind ship Pueraria lobota is 5-20：1-5, the solvent extracted when
Between be 0.5-3 hour, the extraction temperature of the solvent is 50-100 DEG C, and the concentration of the wind ship Pueraria lobota extract is 0.0625mg/ml
More than.
Via technical characteristic of the invention, wind ship Pueraria lobota extract of the invention can be reasonably resistant to injury caused by ultraviolet light
Or promote the skin repair of ultraviolet light harms, therefore have effects that prevent or treat ultraviolet light harms.
Embodiments of the present invention are further illustrated below in conjunction with schema, and following cited embodiments are to illustrate
The present invention, the range being not intended to limit the invention is any to be familiar with this those skilled in the art, is not departing from the spirit and scope of the present invention
It is interior, when can do it is a little change and retouch, therefore protection scope of the present invention when regard appended claims institute's defender as
It is quasi-.
Detailed description of the invention
Fig. 1 is the experimental result that for wind ship Pueraria lobota fruit extract dermal fibroblasts are resisted with the effect of UV.
Fig. 2 is the experimental result that for wind ship Pueraria lobota fruit extract dermal fibroblasts are defendd with the effect of UV.
Fig. 3 is that (* * represents p for the wind ship Pueraria lobota fruit extract of the experimental result influence for to(for) antioxidant genes<0.01).
Fig. 4 is for the fluorescent staining figure of wind ship Pueraria lobota fruit extract repair cell injury experiment.Green：Through Annexin V
Dyeing, shows the cell of slightly damaged；It is red：It is dyed through PI, shows the nucleus being badly damaged；Blue：Through Hoechst
33342 dyeing, are nucleus counterstains.
Fig. 5 is for wind ship Pueraria lobota Essence in the protection UVA Efficacy experiments of skin, experimental result (the * generation of dermatoglyph
Table was relative to the 0th week p<0.05).
Fig. 6 is for wind ship Pueraria lobota Essence in the protection UVA Efficacy experiments of skin, the experimental result of skin pore size
(* is represented relative to the 0th week p<0.05).
Specific embodiment
The present invention, which provides a kind of wind ship Pueraria lobota extract and is used to prepare, to be promoted the performance of SOD2 gene, promotes skin repair and prevention
For skin by the purposes of the composition of ultraviolet light harms, wind ship Pueraria lobota extract of the invention is to be extracted with wind ship Pueraria lobota fruit with solvent
It takes and is centrifuged and obtain, can be used for being promoted the performance of SOD2 gene, promote skin repair and prevention skin by ultraviolet light harms.
Meanwhile the present invention is used to prepare and promotes the performance of SOD2 gene, skin repair and prevention skin is promoted to be hurt by ultraviolet light
Harmful composition, also may include an a effective amount of wind ship Pueraria lobota extract and a pharmaceutically acceptable carrier, the composition be with
Powdered, graininess, liquid, glue or paste exist, and its dosage form is with food, drink, drug, reagent or nutritional supplement
Form provide.
The detailed extracting process that will be detailed below wind ship Pueraria lobota extract of the present invention, the skin with the wind ship Pueraria lobota extract
The test experiments of the uva-resistant ability of fibroblast, antioxidant genes ability, to confirm the wind ship Pueraria lobota extract for promoting SOD2
Gene performance promotes skin repair and prevents skin by the effect of ultraviolet light harms.
Wind ship Pueraria lobota extract preparation flow：
1. wind ship Pueraria lobota fruit cleans, for subsequent extraction；
2. taking the wind ship Pueraria lobota fruit after cleaning with 5-20：The liquid-solid ratio of 1-5, after homogeneous in a solvent in 50-100 DEG C of difference
Extraction 0.5-3 hours is carried out, which is water, alcohols, aqueous alcohols or combinations thereof；
3. being cooled to room temperature；
4. via 400mesh strainer filtering；
5. being concentrated under reduced pressure in 45~70 DEG C to get extract liquor.
The uva-resistant ability test of 1 dermal fibroblasts of embodiment：
Experiment equipment and material：
1. cell strain：Human skin fibroblast (CCD-966sk；BCRC No.60153)；
2. minimum required culture solution (Eagle) adds Earle's BSS, 0.1mM nonessential amino acid, 1.5g/L bicarbonate
Sodium, 1mM Sodium Pyruvate；10% fetal calf serum (Gibco)；
3.4mg/ml 3- (4,5- dimethylthiazole -2) -2,5- diphenyltetrazolium bromide bromide (MTT；AMERSCO/0793-
It 5G) is dissolved in phosphate buffer solution (PBS)；
4. dimethyl sulfoxide (DMSO；ECHO/DA1101-000000-72EC)；
5. ferment immunity analysis instrument (ELISA reader；BioTek)；
6. UV irradiation chamber (Ultravioler radiation chamber；Vilber)；
Experimental procedure：
1. being implanted into 5 × 10 in every 200 μ l culture solution of hole in 96 hole culture plates3A cell；
2. being cultivated 24 hours in 37 DEG C；
3. removing culture solution in the case where not disturbing and attaching cell；
4. handling (n=4) with reagent and then being cultivated 24 hours with 37 DEG C；
5. above-mentioned culture plate is irradiated 12J/cm using UV irradiation chamber2Ultraviolet light 1 hour (this dosage is LD50 agent
Amount represents the dosage for making half cell-lethal)；
6. adding the MTT solution in 15 holes μ l/, then cultivated 4 hours in 37 DEG C；
7. removal culture solution simultaneously adds the DMSO in 50 holes μ l/ to remove crystallization；
It is cultivated 10 minutes 8. culture plate is put on oscillator；
9. measuring the suction brightness (O.D value) of wavelength 570nm；
10. the MTT of cell survival rate is analyzed：
Cell survival rate (%)=(experimental group O.D value/control group O.D value) × 100%；
11. carrying out t-test statistics with Excel software.
Experiment is divided into defence UV group and resists two kinds of test groups of UV, resistance group be for after test cell irradiating ultraviolet light,
Whether can reach the effect of anti-UV using wind ship Pueraria lobota extract；Defence group be first ultraviolet using being irradiated after wind ship Pueraria lobota extract again
Light tests whether to can reach the injury for preventing UV.Above step is to defend the testing procedure of UV group, and resist the group of UV to incite somebody to action
The 4th, 5 steps are exchanged in above-mentioned steps.
Experimental result such as Fig. 1 of the effect of UV, figure are resisted to dermal fibroblasts and defendd to wind ship Pueraria lobota fruit extract
2.By the processing of wind ship Pueraria lobota fruit extract, cell mortality is decreased obviously, and display wind ship Pueraria lobota fruit extract, which has, to be resisted
And the effect of defence UV.
The detection of 2 dermal fibroblasts antioxidant genes ability of embodiment：
Laboratory apparatus and material：
1. cell：Mankind's fibroblast (CCD-996SK；ATCC)；
2. culture solution：Minimum required culture solution (Eagle) addition Earle's balanced salt solution (Earle's BSS),
0.1mM nonessential amino acid, 1.5g/L sodium bicarbonate, 1mM Sodium Pyruvate, account for 90%；Fetal calf serum 10% (Gibco)；
3.RNA extracts set group (Geneaid)；
4.III reverse transcriptase (Invitrogen)；
5. anti-oxidant related gene introduction group (UNG, OGG1, APE1, XRCC1, XRCC5, MSH2, MLH1, MSH6,
ERCC1, XPA, SOD1, SOD2, GPx, and CAT), it include internal control；
6.KAPA CYBR FAST qPCR set group (2x) (KAPA Biosystems)；
7. real time aggregation enzyme chain reaction instrument (Step One Plus；ABI)；
Experimental procedure：
The preparation and stimulation of cell
1. 2ml culture solution and implantation 1.5x10 is added in every hole in 6 porose discs5The hole cells/；
2. being cultivated 24 hours in 37 DEG C；
3. removing culture solution in the case where not disturbing and attaching cell；
4. handling (n=3) with condition A, B, C and then being cultivated 6 hours with 37 DEG C；
A. placebo (Mock)；
B. only exposure UVA；
C. exposure UVA+ wind ship Pueraria lobota extract；
5. irradiating 5J/cm in UV irradiation chamber system2UVA.
Genetic test：
1. collecting stimulated cell；
2. collecting the RNA of cell with RNA extraction set group；
3. generating the cDNA of RNA using reverse transcriptase；
4. target gene (anti-oxidant pointer gene SOD2) is using ABI StepOne Plus with KAPA SYBR FASt
It carries out qPCR and quantifies；
5. determining the relative quantity of gene performance using 2- Δ Δ Ct method；
6. carrying out non-paired list tail Student t- detection using Excel software to determine the coefficient of variation and whether there is system
There is significant difference (* p value on meter<0.05；* p value<0.01；* * p value<0.001).
Sunlight middle-ultraviolet lamp stimulates the generation of free radical (ROS), so that skin is easy aging, generated wrinkle, while causing DNA
Damage, increases the risk of cutaneum carcinoma, if oxidation resistance can be promoted, prevents free radical (ROS) from generating, can reduce above-mentioned injury
Generation.Experimental result such as Fig. 3 of influence of the wind ship Pueraria lobota fruit extract for antioxidant genes is extracted by wind ship Pueraria lobota fruit
The processing of object, oxidation resistance are obviously improved, and display wind ship Pueraria lobota fruit extract can reduce purple by promoting oxidation resistance
Injury of the outside line to skin.
3 dermal fibroblasts injury repair fluorescent test of embodiment：
1. cell strain：Human skin fibroblast (CCD-966sk；BCRC No.60153)；
2. culture solution：
Minimum required culture solution (Eagle) addition Earle's BSS, 0.1mM nonessential amino acid (90%),
1.5g/L sodium bicarbonate, 1mM Sodium Pyruvate, fetal calf serum 10% (Gibco)；
3. phosphate buffer solution (Gibco)；
4. propidium iodide stain solution (BD Pharmingen；Cat.51-66211E)；
5.Hoechst 33342(Thermo；Cat.62249)；
6.Annexin V,Alexa Fluor 488(Invitrogen；Cat.A13201)；
7.Annexin V is bonded buffer solution (eBioscience；Cat.00-0055-43)；
8. UV irradiation chamber (Vilber)；
9. microscope (ZEISS)；
Experimental procedure：
1. being implanted into 2 × 10 in 24 porose discs4Cells/well, and it is overnight in 37 DEG C of cultures；
2. small to carry out pretreatment 24 in 0.5mg/ml wind ship Pueraria lobota fruit extract addition culture solution before UVA irradiation
When；
3. using UV irradiation chamber with 12J/cm2It irradiates overnight；
4. by cell with ofpropidium iodide solution (1 in Annexin V bond buffer:250) with Annexin V (1:
250) it dyes 15-30 minutes；
5. with Hoechst 33342 (1:20000) by cell dyeing 3 minutes；
6. with phosphate buffer cleaning 2 times；
7. under the microscope in fluorescence microscopy.
Fig. 4 is the fluorescence expression figure of dermal fibroblasts injury repair after the effect of wind ship Pueraria lobota fruit extract, by wind
The processing of ship Pueraria lobota fruit extract, hence it is evident that can be seen that cell is repaired.
The protection UVA effect of 4 wind ship Pueraria lobota Essence of embodiment for skin
To confirm that wind ship Pueraria lobota extract of the invention to protection UVA effect of skin, prepares 5% wind ship Ge Cui of addition respectively
Take object contains 5% wind ship Pueraria lobota Essence；And the Essence as a control group of the Essence without wind ship Pueraria lobota extract, the wherein essence
Magnificent liquid ingredient is all water, fragrant fresh ketone, hexylene glycol, 1,3 butylene glycol, Xanthan gum, thickener and triethanolamine.Then, by tested
Control group Essence, 5% wind ship Pueraria lobota Essence are used facial skin by 10 people of person respectively, each daily morning and evening to use once, and every
Subject is applied to face using control group Essence or 5% wind ship Pueraria lobota Essence respectively after having cleaned face daily morning and evening
(take essence liquid measure is that pressure head presses to portion's skin, then Essence is uniformly applied to forehead skin, and slightly with finger pulp
Massage promote absorb), and after use the 0th (before use), progress skin quality measurement in 1,2 and 4 week (with Skin Analyzer TD instrument
Device detects dermatoglyph and state of pores), tested period receives general daily solarization.
Skin-texture detection result such as Fig. 5, it can be seen that be effectively improved dermatoglyph using 5% wind ship Pueraria lobota Essence and reach
31.8%.In addition, the testing result for skin pore is as shown in Figure 6, it can be seen that 5% wind ship Pueraria lobota Essence is effectively improved skin
Pore is up to 32.5%.
The wind ship Pueraria lobota extract as shown in above-mentioned experiment, can be by the promotion of oxidation resistance, and then resists and defend UV, and
And cell can be made to be repaired, it is finally reached the effect of anti-light aging.
In conclusion either showing in cell experiment and gene, the wind ship Pueraria lobota extract of this case, which all has, promotes SOD2
Gene performance promotes the effect of skin repair and prevention skin are by ultraviolet light harms, therefore, can be as promotion SOD2 gene table
Existing, promotion skin repair and prevention skin are by the food of ultraviolet light harms, drink, drug, reagent or nutritional supplement etc..
Claims (10)
1. a kind of wind ship Pueraria lobota extract is used to prepare the purposes for promoting the composition of SOD2 gene performance, wherein the wind ship Pueraria lobota is extracted
Object is that wind ship Pueraria lobota is extracted with the solvent of water, alcohol or aqueous alcohols and obtained.
2. a kind of wind ship Pueraria lobota extract is used to prepare the purposes for promoting the medical composition of skin repair, wherein the wind ship Pueraria lobota is extracted
Object is that wind ship Pueraria lobota is extracted with the solvent of water, alcohol or aqueous alcohols and obtained.
3. a kind of wind ship Pueraria lobota extract is used to prepare prevention skin by the purposes of the medical component of ultraviolet light harms, wherein the wind
Ship Pueraria lobota extract is that wind ship Pueraria lobota is extracted with the solvent of water, alcohol or aqueous alcohols and obtained.
4. purposes described in any one of claim 1 to 3, which is characterized in that the wind ship Pueraria lobota extract is wind ship Pueraria lobota
Fruit extracted and obtained with the solvent of water, alcohol or aqueous alcohols.
5. purposes described in any one of claim 1 to 3, which is characterized in that the composition further includes a doctor
Acceptable carrier on medicine.
6. purposes described in any one of claim 1 to 3, which is characterized in that the composition is powder, particle, liquid
Body, glue or dosage form paste.
7. purposes according to claim 1, which is characterized in that the concentration of the wind ship Pueraria lobota extract is 0.0625mg/ml
More than.
8. purposes described in any one of claim 1 to 3, which is characterized in that the volume ratio of the solvent and wind ship Pueraria lobota
For 5-20：1-5.
9. purposes described in any one of claim 1 to 3, which is characterized in that the time that the solvent is extracted is
0.5-3 hours.
10. purposes described in any one of claim 1 to 3, which is characterized in that the extraction temperature of the solvent is 50-
100℃。
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TW106117454A TWI617311B (en) | 2017-05-25 | 2017-05-25 | Use of cardiospermum halicacabum extract in the manufacture of a composition for upregulating sod2 expression, promoting skin repair and preventing skin damage by uv light |
| TW106117454 | 2017-05-25 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108926623A true CN108926623A (en) | 2018-12-04 |
Family
ID=62189007
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810494237.8A Pending CN108926623A (en) | 2017-05-25 | 2018-05-22 | Application of anemone extract in preparation of composition for improving SOD2 gene expression, repairing skin and preventing ultraviolet light damage |
Country Status (3)
| Country | Link |
|---|---|
| KR (1) | KR20200067988A (en) |
| CN (1) | CN108926623A (en) |
| TW (1) | TWI617311B (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106511580A (en) * | 2017-01-04 | 2017-03-22 | 广州聚澜健康产业研究院有限公司 | Itching relieving composition and application thereof |
-
2017
- 2017-05-25 TW TW106117454A patent/TWI617311B/en active
-
2018
- 2018-05-22 CN CN201810494237.8A patent/CN108926623A/en active Pending
- 2018-05-25 KR KR1020180059335A patent/KR20200067988A/en unknown
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106511580A (en) * | 2017-01-04 | 2017-03-22 | 广州聚澜健康产业研究院有限公司 | Itching relieving composition and application thereof |
Non-Patent Citations (4)
| Title |
|---|
| MING-HSING HUANG等: "Antioxidant and anti-inflammatory properties of Cardiospermum halicacabum and its reference compounds ex vivo and in vivo", 《JOURNAL OF ETHNOPHARMACOLOGY》 * |
| RAMACHANDRAN JEYADEVI等: "Anti-arthritic activity of the Indian leafy vegetable Cardiospermum halicacabum in Wistar rats and UPLC–QTOF–MS/MS identification of the putative active phenolic components", 《INFLAMMATION RESEARCH》 * |
| 吕斌等: "三角泡佐治带状疱疹28例分析", 《现代诊断与治疗》 * |
| 陈君等: "广西倒地铃体外抗氧化活性的谱效关系研究", 《中国药房》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| TWI617311B (en) | 2018-03-11 |
| KR20200067988A (en) | 2020-06-15 |
| TW201900202A (en) | 2019-01-01 |
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| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20190926 Address after: No. 9, Lane 285, Tiangong Road, Jinshan Industrial Zone, Shanghai Applicant after: BAIYUETE MEFU BIOTECHNOLOGY (SHANGHAI) CO.,LTD. Address before: Taipei City, Taiwan, China Applicant before: TCI Co.,Ltd. |
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Application publication date: 20181204 |