CN108925428A - Succulent gold mountainous region rose quick breeding by group culture method - Google Patents

Abstract

The invention discloses a kind of succulent gold mountainous region rose quick breeding by group culture methods, include the following steps: S1, obtain aseptic explant: the terminal bud of gold mountainous region rose being cut into 5cm long, is successively soaked in 70% alcohol and 3% liquor natrii hypochloritis；S2, induction sprout culture: in the MS culture medium MS+6-BA 1mg/l+NAA 0.5mg/l of bacterium that the explant access that the terminal bud in S1 is cut into 1cm long has been gone out；S3: increment culture: 3-5cm sprout is grown to the explant in S2, the explant that 1cm long is again cut into it in taking-up accesses in the MS increment culture medium MS+6-BA 1mg/l+NAA0.5mg/l+AD 0.1mg/l for bacterium of having gone out；S4, quickly increment: 3-5cm sprout is grown to the explant in S3, repeats S3；S5, culture of rootage: cutting the sprout of value-added 3cm or more, accesses in MS root media MS+6-BA 0.1mg/l+IBA0.5mg/l；S6, transplantation of seedlings of taking root: when the sprout generation root long degree under growth root culture medium reaches 2cm or more, it is transplanted to greenhouse potted plant management；The present invention have it is easy to operate, the period is short, greatly improves gold mountainous region rose fertility.

Description

Tissue culture rapid propagation method of succulent plant rose gold

technical field

The invention belongs to the field of plant biotechnology engineering, and in particular relates to a method for tissue culture and rapid propagation of a succulent plant rose gold.

Background technique

The golden mountain rose is a succulent in the sedum family. The scientific name is Greenovia aurea, which is native to the Canary Islands and Tenerife Islands off the northwest coast of Africa. Golden mountain rose is a small and medium-sized succulent variety. The plant is rosette-shaped, with a diameter of 8-25cm. The leaves are tightly arranged in a cup shape. The leaves are obovate or spoon-shaped, and the leaves are light green to yellow-green. Because during the dormant period, in order to avoid adverse environments such as strong light and extreme heat, the leaves in the center of the plant will be tightly wrapped together, and the plant shape is just like green roses that are about to bloom, "green roses that never fade" It is an important reason why people love it. However, in the natural state, the seed setting rate of the golden mountain rose is very low, about 21%. Although the reproductive rate of the plump seeds itself exceeds 85%, it is limited by the low seed setting rate, so the overall reproductive rate of the golden mountain rose is low. Therefore, the market price of potted seedlings of golden mountain roses is relatively high. There is a huge market demand for improving the reproduction of golden mountain roses through technical means. In view of this, the present invention is proposed.

Invention

Aiming at the problems existing in the prior art, the object of the present invention is to provide a method for the rapid propagation of the succulent plant rose gold by tissue culture, which is easy to operate and has a short cultivation cycle, which greatly improves the reproductive capacity of the rose gold and greatly improves the reproduction coefficient. .

In order to achieve the above object, a kind of succulent plant golden mountain rose tissue culture rapid propagation method provided by the invention comprises the following steps:

S1, obtaining sterile explants: cutting the terminal buds of the Golden Mountain Rose into 5 cm long, soaking in 70% alcohol and 3% sodium hypochlorite solution successively;

S2. Induced bud culture: cut the terminal buds in S1 into 1 cm long explants and insert them into MS medium MS+6-BA 1mg/l+NAA 0.5mg/l;

S3: Value-added culture: After the explants in S2 grow 3-5cm buds, take them out and cut them into 1cm-long explants and insert them into the sterilized MS value-added medium MS+6-BA 1mg/l +NAA 0.5mg/l+AD 0.1mg/l;

S4, rapid increase in value: the explants in S3 grow 3-5cm buds, repeat S3, and carry out rapid propagation of buds;

S5, rooting culture: cut off the buds with a value of more than 3cm, and insert them into MS rooting medium MS+6-BA 0.1mg/l+IBA0.5mg/l;

S6. Transplanting of rooted seedlings: when the root length of the sprouts on the rooting medium reaches more than 2 cm, they are transplanted to the greenhouse for potted management.

Preferably, the S1-S5 are all operated on a sterile operating table;

Preferably, the terminal buds of the Golden Mountain Rose need to be rinsed under tap water for 2 hours before performing the S1;

Preferably, the immersion time of the terminal buds in alcohol and sodium hypochlorite solution in the S1 is 30 seconds and 10 minutes successively;

Preferably, in the S6, the seedlings whose roots grow to more than 2 cm are removed in time to peat soil: garden soil: perlite=6: 2: 2 for potted management;

Preferably, the medium is sterilized.

Provided by the present invention has the following beneficial effects:

The method for rapid propagation of the succulent plant Rosaceae rosea by tissue culture is easy to operate. Firstly, the explants of Roses roses are processed and aseptically inserted into tubes to obtain aseptic explants; then the buds of Roses roses are processed Induction, the sterile bud explants are inserted into the induction bud medium to obtain clustered buds; the induced buds are inserted into the value-added medium to further expand the induced buds; the golden mountain rose buds are inserted into the rooting medium for growth induction Rooting culture: Transplant the rooted golden mountain roses of a certain size in the test tube to a greenhouse for management. Through the combination of nutrients and hormones, the reproduction coefficient of Golden Mountain Rose is 4.7, that is, one bud explant can be value-added to obtain 4.7 buds through tissue culture, which achieves a short cultivation cycle, greatly improves the reproductive ability of Golden Mountain Rose, and greatly improves the reproduction coefficient. Propagation of large, rapid golden mountain roses in test tubes.

Detailed ways

The present invention will be further described below in conjunction with specific embodiments, so as to help understand the contents of the present invention.

The present invention provides a kind of succulent plant golden mountain rose tissue culture rapid propagation method, comprising the following steps:

S1. Materials processing: take the top buds of the golden mountain rose and wash them under tap water for 2 hours;

S2. Obtain aseptic explants: on the aseptic operating table, cut the terminal buds of the golden mountain rose into 5 cm lengths, soak them in 70% alcohol for 30 seconds, and then place them in 3% sodium hypochlorite solution for sterilization for 10 minutes.

S3. Induced bud culture: On the aseptic operating table, cut the terminal buds of the sterilized Golden Mountain Rose into 1 cm long explants and insert them into the sterilized MS medium MS+6-BA 1mg/ l+NAA 0.5mg/l.

S4. Value-added culture: After about 35 days, the explants will grow 3-5cm buds. Also on the aseptic operation table, take out the explants, and then cut the golden mountain rose into 1cm long explants and insert them into Miehao Bacterial MS proliferation medium MS+6-BA 1mg/l+NAA 0.5mg/l+AD 0.1mg/l.

S5, rapid value-added: After about 35 days, the same explants will have 3-5cm buds to grow. At this time, you can continue to step 4 to carry out the rapid propagation of buds;

S6. Rooting culture: cut off the buds with a value of more than 3 cm, and insert them into MS rooting medium MS+6-BA 0.1 mg/l+IBA 0.5 mg/l.

S7, transplanting of rooted seedlings: After about 20 days, the buds of Rose Gold Mountain on the rooting medium will have roots to generate; the seedlings with roots growing to more than 2 cm are removed to peat soil: garden soil: perlite=6: 2: 2 Pot management in substrate.

In this paper, specific examples are used to illustrate the inventive concept in detail, and the descriptions of the above embodiments are only used to help understand the core idea of the present invention. It should be pointed out that for those skilled in the art, any obvious modification, equivalent replacement or other improvement should be included in the protection scope of the present invention without departing from the inventive concept.

Claims (6)

1. a succulent plant golden mountain rose tissue culture rapid propagation method, is characterized in that, comprises the steps: S1, obtaining sterile explants: cutting the terminal buds of the Golden Mountain Rose into 5 cm long, soaking in 70% alcohol and 3% sodium hypochlorite solution successively; S2. Induced bud culture: the terminal buds in S1 were cut into 1 cm long explants and inserted into MS medium MS+6-BA 1mg/l+NAA 0.5mg/l; S3: Value-added culture: After the explants in S2 grow 3-5cm buds, take them out and cut them into 1cm-long explants and insert them into MS value-added medium MS+6-BA 1mg/l+NAA 0.5mg /l+AD 0.1mg/l; S4, rapid increase in value: the explants in S3 grow 3-5cm buds, repeat S3, and carry out rapid propagation of buds; S5, rooting culture: cut off the buds with a value of more than 3cm, and insert them into MS rooting medium MS+6-BA 0.1mg/l+IBA0.5mg/l; S6. Transplanting of rooted seedlings: when the root length of the sprouts on the rooting medium reaches more than 2 cm, they are transplanted to the greenhouse for potted management. 2. a kind of succulent plant golden mountain rose tissue culture rapid propagation method according to claim 1, is characterized in that, described S1-S5 are all operated on aseptic operation bench. 3. a kind of succulent plant rose gold tissue culture rapid propagation method according to claim 2, is characterized in that, before carrying out described S1, the terminal bud of rose gold needs to be rinsed under tap water for 2 hours. 4. A kind of succulent plant golden mountain rose tissue culture rapid propagation method according to claim 3, is characterized in that, the immersion time of terminal bud in alcohol and sodium hypochlorite solution in the said S1 is successively 30 seconds and 10 minutes. 5. a kind of succulent plant golden mountain rose tissue culture rapid propagation method according to claim 1, is characterized in that, described medium is all sterilized. 6. a kind of succulent plant golden mountain rose tissue culture rapid propagation method according to claim 1, is characterized in that, in described S6, the seedling that root grows to more than 2cm is removed in time to peat soil: garden soil: perlite= 6:2:2 substrate in potted management.