CN108925428A - Succulent gold mountainous region rose quick breeding by group culture method - Google Patents
Succulent gold mountainous region rose quick breeding by group culture method Download PDFInfo
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- CN108925428A CN108925428A CN201810860572.5A CN201810860572A CN108925428A CN 108925428 A CN108925428 A CN 108925428A CN 201810860572 A CN201810860572 A CN 201810860572A CN 108925428 A CN108925428 A CN 108925428A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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Abstract
The invention discloses a kind of succulent gold mountainous region rose quick breeding by group culture methods, include the following steps: S1, obtain aseptic explant: the terminal bud of gold mountainous region rose being cut into 5cm long, is successively soaked in 70% alcohol and 3% liquor natrii hypochloritis；S2, induction sprout culture: in the MS culture medium MS+6-BA 1mg/l+NAA 0.5mg/l of bacterium that the explant access that the terminal bud in S1 is cut into 1cm long has been gone out；S3: increment culture: 3-5cm sprout is grown to the explant in S2, the explant that 1cm long is again cut into it in taking-up accesses in the MS increment culture medium MS+6-BA 1mg/l+NAA0.5mg/l+AD 0.1mg/l for bacterium of having gone out；S4, quickly increment: 3-5cm sprout is grown to the explant in S3, repeats S3；S5, culture of rootage: cutting the sprout of value-added 3cm or more, accesses in MS root media MS+6-BA 0.1mg/l+IBA0.5mg/l；S6, transplantation of seedlings of taking root: when the sprout generation root long degree under growth root culture medium reaches 2cm or more, it is transplanted to greenhouse potted plant management；The present invention have it is easy to operate, the period is short, greatly improves gold mountainous region rose fertility.
Description
Technical field
The invention belongs to Plant Biotechnology engineering field more particularly to a kind of succulent gold mountainous region rose tissue culture are fast
Fast propagation method.
Background technique
Gold mountainous region rose is the succulent that Crassulaceae palm of lotus belongs to.Scientific name Greenovia aurea originates in Africa
The ground such as Canary Islands, the Tenerife archipelago of northwest seashore.Gold mountainous region rose, belongs to middle-size and small-size fleshiness kind, and plant is in
Existing rosette-stape, diameter is between 8-25cm, and closely at cup-shaped, blade obovate or cochlear, blade light green color arrives blades arrangement
Yellow green.Because it is during suspend mode, in order to hide the adverse environments such as strong light, extremely hot, the blade of plant central part can be wrapped tightly
It is rolled in together, for plant type just like many green roses that a bud just ready to burst, " forever amaranthine green rose " is that people are favorite heavy
Want reason.But under natural conditions, gold mountainous region rose seed-setting rate is very low, and about 21%, although full seed itself
Breeding potential is more than 85%, but is limited by lower seed-setting rate, therefore gold mountainous region rose breeding potential is totally lower.So gold
The mountainous region rose basin seedling market price is higher.The breeding of gold mountainous region rose is improved by technological means, there is the huge market demand.Have
In consideration of it, the present invention is specifically proposed.
In invention
Aiming at the problems existing in the prior art, the object of the present invention is to provide a kind of succulent gold mountainous region rose groups
Fast tissue culture reproducing method, easy to operate, cultivation period is short, greatly improves gold mountainous region rose fertility, makes line of breeding
Number greatly improves.
To achieve the goals above, a kind of succulent gold mountainous region provided by the invention rose quick breeding by group culture side
Method includes the following steps:
S1, obtain aseptic explant: the terminal bud of gold mountainous region rose is cut into 5cm long, be successively soaked in 70% alcohol and
3% liquor natrii hypochloritis；
S2, induction sprout culture: the explant that the terminal bud in S1 is cut into 1cm long is accessed to the MS culture medium MS+ for bacterium of having gone out
In 6-BA 1mg/l+NAA 0.5mg/l；
S3: increment culture: 3-5cm sprout is grown to the explant in S2, the explant that 1cm long is again cut into it in taking-up connects
In the MS increment culture medium MS+6-BA 1mg/l+NAA 0.5mg/l+AD 0.1mg/1 of the good bacterium of death of monks or nuns；
S4, quickly increment: growing 3-5cm sprout to the explant in S3, repeat S3, carries out sprout and quickly breeds；
S5, culture of rootage: cutting the sprout of value-added 3cm or more, accesses MS root media MS+6-BA 0.1mg/l
In+IBA0.5mg/l；
S6, transplantation of seedlings of taking root: it when the sprout generation root long degree under growth root culture medium reaches 2cm or more, is transplanted to
Greenhouse potted plant management.
Preferably, the S1-S5 is operated on aseptic operating platform；
Preferably, the terminal bud by gold mountainous region rose is needed to rinse 2 hours under tap water before carrying out the S1；
Preferably, soaking time of the terminal bud in alcohol and liquor natrii hypochloritis is followed successively by 30 seconds and 10 points in the S1
Clock；
Preferably, the seedling of root long to 2cm or more is moved to peat soil in time in the S6: garden mould: perlite=6: 2: 2
Matrix in potted plant management；
Preferably, the culture medium passes through sterilization treatment.
It is provided by the invention, it has the following beneficial effects:
Succulent gold provided by the invention mountainous region rose quick breeding by group culture method, it is easy to operate, first to gold mountain
The processing of ground rose explant and the processing of sterile inlet pipe are to obtain acquisition aseptic explant；Sprout is carried out to gold mountainous region rose again to lure
It leads, sterile bud explant is accessed induced bud culture medium, obtains Multiple Buds；Induced bud is accessed increment culture medium, to induced bud
Further expansion breeding；Gold mountainous region rose bud is accessed root media, carries out raw root induction culture；Certain in test tube
Size, the gold mountainous region rose that has taken root transplants and manages into greenhouse.Pass through the combination of nutrition and hormone, gold mountainous region rose
Breeding coefficient is that 4.7 i.e. bud explant can be rised in value by tissue culture and obtain 4.7 buds, and it is short to reach cultivation period, is greatly improved
Gold mountainous region rose fertility, greatly improves breeding coefficient, carries out a large amount of, quick gold mountainous region rose in test tube
Breeding.
Specific embodiment
The present invention will be further described combined with specific embodiments below, to help the contents of the present invention are understood.
The present invention provides a kind of succulent gold mountainous region rose quick breeding by group culture method, includes the following steps:
S1, materials processing: the terminal bud of gold mountainous region rose is taken, rinses 2 hours under tap water；
S2, it obtains aseptic explant: on aseptic operating platform, the terminal bud of gold mountainous region rose being cut into 5cm long, with 70%
Alcohol rapid soaking 30 seconds, and be placed on 3% liquor natrii hypochloritis and sterilize 10 minutes.
S3, induction sprout culture: on aseptic operating platform, the terminal bud of the gold mountainous region rose for bacterium of having gone out is cut into 1cm again
Long explant accesses in the MS culture medium MS+6-BA 1mg/l+NAA 0.5mg/l for bacterium of having gone out.
S4, increment culture: explant has 3-5cm sprout and grows after about 35 days, equally on aseptic operating platform, takes out outer
Implant, then gold mountainous region rose be cut into 1cm long explant access gone out bacterium MS rise in value culture medium MS+6-BA 1mg/l+
In NAA 0.5mg/l+AD 0.1mg/1.
S5, quickly increment: after about 35 days, same explant has 3-5cm sprout and grows, and can proceed with step 4 at this time,
Sprout is carried out quickly to breed；
S6, culture of rootage: cutting the sprout of value-added 3cm or more, accesses MS root media MS+6-BA 0.1mg/l
In+IBA0.5mg/l.
S7, transplantation of seedlings of taking root: after about 20 days, the sprout of gold mountainous region rose has root generation on root media；By root
The long seedling to 2cm or more is moved to peat soil: garden mould: perlite=6: potted plant management in 2: 2 matrix.
Specific case used herein elaborates inventive concept, the explanation of above example is only intended to
Help understands core of the invention thought.It should be pointed out that for those skilled in the art, not departing from this
Under the premise of inventive concept, any obvious modification, equivalent replacement or the other improvements made should be included in the present invention
Protection scope within.
Claims (6)
1. a kind of succulent gold mountainous region rose quick breeding by group culture method, which comprises the steps of:
S1, it obtains aseptic explant: the terminal bud of gold mountainous region rose being cut into 5cm long, is successively soaked in 70% alcohol and 3% time
Sodium chlorate solution；
S2, induction sprout culture: the explant that the terminal bud in S1 is cut into 1cm long is accessed into MS culture medium MS+6-BA 1mg/l+
In NAA 0.5mg/l；
S3: increment culture: growing 3-5cm sprout to the explant in S2, takes out the explant access MS for it being cut into again 1cm long
In increment culture medium MS+6-BA 1mg/l+NAA 0.5mg/l+AD 0.1mg/l；
S4, quickly increment: growing 3-5cm sprout to the explant in S3, repeat S3, carries out sprout and quickly breeds；
S5, culture of rootage: cutting the sprout of value-added 3cm or more, accesses MS root media MS+6-BA 0.1mg/l+
In IBA0.5mg/l；
S6, transplantation of seedlings of taking root: when the sprout generation root long degree under growth root culture medium reaches 2cm or more, it is transplanted to greenhouse
Potted plant management.
2. a kind of succulent gold mountainous region rose quick breeding by group culture method according to claim 1, which is characterized in that
The S1-S5 is operated on aseptic operating platform.
3. a kind of succulent gold mountainous region rose quick breeding by group culture method according to claim 2, which is characterized in that
The terminal bud by gold mountainous region rose is needed to rinse 2 hours under tap water before carrying out the S1.
4. a kind of succulent gold mountainous region rose quick breeding by group culture method according to claim 3, which is characterized in that
Soaking time of the terminal bud in alcohol and liquor natrii hypochloritis is followed successively by 30 seconds and 10 minutes in the S1.
5. a kind of succulent gold mountainous region rose quick breeding by group culture method according to claim 1, which is characterized in that
The culture medium passes through sterilization treatment.
6. a kind of succulent gold mountainous region rose quick breeding by group culture method according to claim 1, which is characterized in that
The seedling of root long to 2cm or more is moved to peat soil in time in the S6: garden mould: perlite=6: potting pipe in 2: 2 matrix
Reason.
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| CN103270947A (en) * | 2013-03-08 | 2013-09-04 | 浙江省农业科学院 | Duvalia angustiloba tissue culturing method |
| CN104273028A (en) * | 2013-07-03 | 2015-01-14 | 中国科学院上海生命科学研究院 | Method for rapid in-vitro propagation of Crassulaceae plant |
| CN105145354A (en) * | 2015-09-08 | 2015-12-16 | 浙江省农业科学院 | Method for aseptically seeding ebony and establishing leaf-cutting rapid propagation system |
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| CN105409773A (en) * | 2015-11-27 | 2016-03-23 | 浙江省农业科学院 | Lophophora williamsii sterile seeding and regeneration system establishing method |
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