CN108925368A - A kind of method of saussurea involucrata mushroom strains fast-propagation - Google Patents
A kind of method of saussurea involucrata mushroom strains fast-propagation Download PDFInfo
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- CN108925368A CN108925368A CN201810658859.XA CN201810658859A CN108925368A CN 108925368 A CN108925368 A CN 108925368A CN 201810658859 A CN201810658859 A CN 201810658859A CN 108925368 A CN108925368 A CN 108925368A
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- saussurea involucrata
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- involucrata mushroom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
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Abstract
The invention discloses a kind of methods of saussurea involucrata mushroom strains fast-propagation, the following steps are included: S1, the preparation of fluid nutrient medium: every liter of the fluid nutrient medium includes: the potato of 180-220g peeling chopping, 40-60g maize flour, 20-40g glucose, 2-4g potassium dihydrogen phosphate, 1-2g magnesium sulfate, 12-16mg vitamin B, above-mentioned material plus 1800-2200mL distilled water Hybrid Heating are boiled into 30min, and filter constant volume 900-1100mL, adjust pH to 6.5-7.5, and it is uniformly sub-packed in 8 500mL conical flasks, sealing is with 120 DEG C of high pressure sterilization 25-35min, taking-up cools.The present invention can satisfy the original seed demand that cultivation amount is big, the plantation time is long, while activate to original seed, improves original seed to the adaptability of environment, and shorten the fruiting time of cultivation saussurea involucrata mushroom, improves saussurea involucrata mushroom final product quality, be worthy to be popularized.
Description
Technical field
The present invention relates to edible mushroom culture technique field more particularly to a kind of methods of saussurea involucrata mushroom strains fast-propagation.
Background technique
Edible mushroom is the general designation of large altitude fungi that can be edible for humans.According to statistics, there is 7000 kinds true in the whole world
Mushroom entity is edible, but kind by success domesticating and cultivating only more than 60, and only 33 kinds reach and commercially produce
Scale.
Saussurea involucrata mushroom also known as Agroeybe cylindracea mycelia, Liu Huan bacterium, Liu Songrong.Saussurea involucrata mushroom mushroom body is pure white, and when mature, food fiber exists
Content in fructification is using stem as highest, thus the cap of saussurea involucrata mushroom and stem can give different flavor and texture, lid
Fertile handle is crisp, and excellent taste is full of nutrition.According to data, mushroom body contains reduced sugar 29.96%, total reducing sugar 54.36%, crude protein
27.51%, amino acid content is abundant, and eight kinds of essential amino acids of human body are complete, and wherein lysine content is up to 1.75%.Therefore,
Saussurea involucrata mushroom is a kind of very high agaric of edible value.
In order to make saussurea involucrata mushroom become a kind of outstanding industrial crops, promotion saussurea involucrata mushroom scale cultivation shortens its and plants the period,
Fast-propagation to original seed is the Cultivating techniques being badly in need of now.Universal fast-propagation technology will solve saussurea involucrata mushroom scale and cultivate early period
Technical problem, have important role to the cultivation of strain thereafter.
Summary of the invention
The purpose of the present invention is to solve disadvantages existing in the prior art, and a kind of saussurea involucrata mushroom strains proposed are quick
Expand numerous method.
A kind of method of saussurea involucrata mushroom strains fast-propagation, comprising the following steps:
The preparation of S1, fluid nutrient medium: every liter of the fluid nutrient medium includes: potato, the 40- of 180-220g peeling chopping
60g maize flour, 20-40g glucose, 2-4g potassium dihydrogen phosphate, 1-2g magnesium sulfate, 12-16mg vitamin B, by above-mentioned material plus
1800-2200mL distilled water Hybrid Heating boils 30min, and filters constant volume 900-1100mL, adjusts pH to 6.5-7.5, and
Even to be sub-packed in 8 500mL conical flasks, sealing is cooled with 120 DEG C of high pressure sterilization 25-35min, taking-up；
S2, inoculation: it in an aseptic environment, takes the saussurea involucrata mushroom parent species solid medium of 3 0.4-0.6g to be respectively put into and is equipped with
In the conical flask of fluid nutrient medium, it is placed in surface；
S3, stationary culture: fluid nutrient medium made from step S2 is sealed, 25 DEG C of constant incubator stationary cultures are put into
45-50h, until its surface layer generates white circular spot；
S4, shaking table culture: fluid nutrient medium made from step S3 is put into 25 DEG C of constant-temperature tables, with the revolving speed of 180r/min
96-120h is cultivated, until liquid is clarified in culture medium, white hypha ball is intensive and uniform；
S5, it inoculates: taking 10-15mL liquid bacteria drop to enter in fluid nutrient medium made from step S4, shake up, repeat step
The shaking table culture step in stationary culture and step S4 in S3.
Preferably, it in the S2, is not required to shake up after fluid nutrient medium inoculation.
Preferably, in the S3, after being put into 25 DEG C of constant incubator stationary culture 45-50h, if its surface layer generates other face
The spot of color, gives up and does not have to.
Preferably, in the S4, white hypha bulb diameter is 2-3mm in liquid bacterium solution.
Compared with prior art, the beneficial effects of the present invention are:
1, original seed is activated, to improve original seed to the adaptability of environment；
2, shorten the fruiting time of cultivation saussurea involucrata mushroom；
3, saussurea involucrata mushroom final product quality is improved.
The present invention program is the quick propagation method of original seed needed for saussurea involucrata mushroom batch is cultivated, and can satisfy that cultivation amount is big, plants
Kind of time long original seed demand, while in the training period, activates original seed, to improve original seed to the adaptability of environment,
And shorten the fruiting time of cultivation saussurea involucrata mushroom, saussurea involucrata mushroom final product quality is improved, is worthy to be popularized.
Specific embodiment
Combined with specific embodiments below the present invention is made further to explain.
Embodiment one:
A kind of method of saussurea involucrata mushroom strains fast-propagation comprising the steps of:
The preparation of S1, fluid nutrient medium: every liter of the fluid nutrient medium includes: removing the peel potato 200g, the maize flour of chopping
50g, glucose 30g, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, vitamin B14 mg, above-mentioned material plus 2000mL distilled water are mixed
It closes to heat to boil 30min and filter and is settled to 1000mL, adjust pH to 7, and be uniformly sub-packed in 8 500mL conical flasks, seal
It is taken out within high pressure sterilization 30 minutes and is cooled with 120 degrees Celsius.
S2, inoculation: in an aseptic environment, the saussurea involucrata mushroom parent species solid medium of 3 about 0.5g weight is taken to be respectively put into 125mL
Fluid nutrient medium (500mL conical flask) surface.
S3, stationary culture: fluid nutrient medium is sealed, and is put into 25 DEG C of constant incubator stationary culture 48h, until is produced from its surface layer
Raw white circular spot.
S4, shaking table culture: fluid nutrient medium is put into 25 DEG C of constant-temperature tables, cultivates 120h with revolving speed 180r/min, until culture
Liquid is clarified in base, and white hypha ball is intensive and uniform.
S5, inoculate: taking 15mL liquid bacteria drop to enter in the fluid nutrient medium newly configured, shake up, repeat stationary culture and
Shaking table culture step.
Embodiment two:
A kind of method of saussurea involucrata mushroom strains fast-propagation comprising the steps of:
The preparation of S1, fluid nutrient medium: every liter of the fluid nutrient medium includes: removing the peel potato 400g, the maize flour of chopping
100g, glucose 60g, potassium dihydrogen phosphate 6g, magnesium sulfate 3g, vitamin B1 8mg mix above-mentioned material plus 4000mL distilled water
Heating, which is boiled 30min and filtered, is settled to 2000mL, adjusts pH to 6.7, and be uniformly sub-packed in 16 500mL conical flasks, seals
Mouth is taken out with 120 degrees Celsius and is cooled for high pressure sterilization 30 minutes.
S2, inoculation: in an aseptic environment, the saussurea involucrata mushroom parent species solid medium of 3 about 0.4g weight is taken to be respectively put into 125mL
Fluid nutrient medium (500mL conical flask) surface.
S3, stationary culture: fluid nutrient medium is sealed, and is put into 25 DEG C of constant incubator stationary culture 50h, until is produced from its surface layer
Raw white circular spot.
S4, shaking table culture: fluid nutrient medium is put into 25 DEG C of constant-temperature tables, cultivates 100h with revolving speed 180r/min, until culture
Liquid is clarified in base, and white hypha ball is intensive and uniform.
S5, inoculate: taking 13mL liquid bacteria drop to enter in the fluid nutrient medium newly configured, shake up, repeat stationary culture and
Shaking table culture step.
Embodiment three:
A kind of method of saussurea involucrata mushroom strains fast-propagation comprising the steps of:
The preparation of S1, fluid nutrient medium: every liter of the fluid nutrient medium includes: removing the peel potato 200g, the maize flour of chopping
50g, glucose 30g, potassium dihydrogen phosphate 3g, magnesium sulfate 1.5g, vitamin B14 mg, above-mentioned material plus 2000mL distilled water are mixed
It closes to heat to boil 30min and filter and is settled to 1000mL, adjust pH to 7.5, and be uniformly sub-packed in 8 500mL conical flasks, seal
Mouth is taken out with 120 degrees Celsius and is cooled for high pressure sterilization 30 minutes.
S2, inoculation: in an aseptic environment, the saussurea involucrata mushroom parent species solid medium of 3 about 0.6g weight is taken to be put into 125mL liquid
Culture medium (500mL conical flask) surface.
S3, stationary culture: fluid nutrient medium is sealed, and is put into 25 DEG C of constant incubator stationary culture 45h, until is produced from its surface layer
Raw white circular spot.
S4, shaking table culture: fluid nutrient medium is put into 25 DEG C of constant-temperature tables, cultivates 120h with revolving speed 180r/min, until culture
Liquid is clarified in base, and white hypha ball is intensive and uniform.
S5, inoculate: taking 10mL liquid bacteria drop to enter in the fluid nutrient medium newly configured, shake up, repeat stationary culture and
Shaking table culture step.
The present invention program is the quick propagation method of original seed needed for saussurea involucrata mushroom batch is cultivated, and can satisfy that cultivation amount is big, plants
Kind of time long original seed demand, while in the training period, activates original seed, to improve original seed to the adaptability of environment,
And shorten the fruiting time of cultivation saussurea involucrata mushroom, improve saussurea involucrata mushroom final product quality.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (4)
1. a kind of method of saussurea involucrata mushroom strains fast-propagation, which comprises the following steps:
The preparation of S1, fluid nutrient medium: every liter of the fluid nutrient medium includes: potato, the 40-60g of 180-220g peeling chopping
Maize flour, 20-40g glucose, 2-4g potassium dihydrogen phosphate, 1-2g magnesium sulfate, 12-16mg vitamin B, by above-mentioned material plus
1800-2200mL distilled water Hybrid Heating boils 30min, and filters constant volume 900-1100mL, adjusts pH to 6.5-7.5, and
Even to be sub-packed in 8 500mL conical flasks, sealing is cooled with 120 DEG C of high pressure sterilization 25-35min, taking-up；
S2, inoculation: in an aseptic environment, the saussurea involucrata mushroom parent species solid medium of 3 0.4-0.6g is taken to be respectively put into equipped with liquid
In the conical flask of culture medium, it is placed in surface；
S3, stationary culture: fluid nutrient medium made from step S2 is sealed, 25 DEG C of constant incubator stationary culture 45- are put into
50h, until its surface layer generates white circular spot；
S4, shaking table culture: fluid nutrient medium made from step S3 is put into 25 DEG C of constant-temperature tables, with the revolving speed culture of 180r/min
96-120h, until liquid is clarified in culture medium, white hypha ball is intensive and uniform；
S5, it inoculates: taking 10-15mL liquid bacteria drop to enter in fluid nutrient medium made from step S4, shake up, repeat in step S3
Stationary culture and step S4 in shaking table culture step.
2. a kind of method of saussurea involucrata mushroom strains fast-propagation according to claim 1, which is characterized in that in the S2, liquid
It is not required to shake up after body culture medium inoculated.
3. a kind of method of saussurea involucrata mushroom strains fast-propagation according to claim 1, which is characterized in that in the S3, put
After entering 25 DEG C of constant incubator stationary culture 45-50h, if its surface layer generates the spot of other colors, gives up and do not have to.
4. a kind of method of saussurea involucrata mushroom strains fast-propagation according to claim 1, which is characterized in that in the S4, liquid
White hypha bulb diameter is 2-3mm in body bacterium solution.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115104481A (en) * | 2021-12-31 | 2022-09-27 | 林礼 | Method for liquid culture of Huang cluster bacterium strain |
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| CN103155785A (en) * | 2011-12-09 | 2013-06-19 | 天津市金三农农业科技开发有限公司 | Establishment method for brown mushroom three-level strain propagation system |
| CN105875198A (en) * | 2016-04-29 | 2016-08-24 | 安发（福建）生物科技有限公司 | Culture method for improving stability of cordyceps militaris strain |
| CN107739224A (en) * | 2017-10-17 | 2018-02-27 | 伊犁师范学院 | It is a kind of quickly to breed fluid nutrient medium of saussurea involucrata mushroom strains and preparation method thereof |
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2018
- 2018-06-25 CN CN201810658859.XA patent/CN108925368A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103155785A (en) * | 2011-12-09 | 2013-06-19 | 天津市金三农农业科技开发有限公司 | Establishment method for brown mushroom three-level strain propagation system |
| CN105875198A (en) * | 2016-04-29 | 2016-08-24 | 安发（福建）生物科技有限公司 | Culture method for improving stability of cordyceps militaris strain |
| CN107739224A (en) * | 2017-10-17 | 2018-02-27 | 伊犁师范学院 | It is a kind of quickly to breed fluid nutrient medium of saussurea involucrata mushroom strains and preparation method thereof |
Non-Patent Citations (3)
| Title |
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| 周全等: "姬松茸液体菌种制备初探", 《食用菌学报》 * |
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| 董洪新等: "杨树菇深层培养的初步研究", 《食用菌学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115104481A (en) * | 2021-12-31 | 2022-09-27 | 林礼 | Method for liquid culture of Huang cluster bacterium strain |
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