CN108918716A - A kind of detection method for neotame content in beverage - Google Patents
A kind of detection method for neotame content in beverage Download PDFInfo
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- CN108918716A CN108918716A CN201810811908.9A CN201810811908A CN108918716A CN 108918716 A CN108918716 A CN 108918716A CN 201810811908 A CN201810811908 A CN 201810811908A CN 108918716 A CN108918716 A CN 108918716A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N30/14—Preparation by elimination of some components
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/86—Signal analysis
- G01N30/8624—Detection of slopes or peaks; baseline correction
- G01N30/8631—Peaks
- G01N30/8634—Peak quality criteria
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Abstract
The invention discloses a kind of detection methods for neotame content in beverage, include the following steps：One, prepare sample treatment liquid, two, prepare the neotame standard solution of various concentration, three, neotame standard solution is detected using liquid chromatogram, draw neotame concentration peak surface curve, four, sample treatment liquid is detected under identical liquid phase chromatogram condition, the peak area of neotame is calculated, the concentration of neotame in sample treatment liquid is calculated according to the standard curve of foundation, calculate the content of neotame in beverage, using the content of this method detection neotame sample, stabilization easy to operate, in precision, the needs of analysis can be fully met in terms of accuracy, it provides a kind of quick, accurate analysis method；Mobile phase is done using 30% acetonitrile and 70% buffer solution, its peak area of neotame sample is good with concentration linear relationship, is easy to detect；Accurate good chromatographic condition is suitble to the detection of most beverages in the market.
Description
Technical field
The invention belongs to beverage ingredient technical field of analysis and detection, and in particular to a kind of inspection for neotame content in beverage
Survey method.
Background technique
Neotame, chemical name are N- [N- (3,3-dimethylbutyl)-L- α-aspartoyl]-L-phenylalanine-1- first
Ester, white crystalline powder are a kind of functional sweeteners containing about 4.5% crystallization water, with pure sweet taste, sweet taste association
With, very close to Aspartame, without the bitter taste and metallic taste of the normal band of other intense sweeteners, sweetness ratio sucrose sweet tea 7000~
13000 times, than 30~60 times of Aspartame sweet tea, solubility of the neotame in room temperature (25 DEG C) is lauched is 12.6g/L, this solubility
Normal production needs can be fully met.
Currently, mainly having the chromatography of ions, capillary electrophoresis, efficient liquid for the measuring method of artificial synthesis edulcorant
Phase chromatography, one mass spectrography of liquid chromatogram etc..Wherein high performance liquid chromatography is primarily directed to neotame, honey element, saccharin sodium, peace
The detection of one or both of match honey, Aspartame.As various countries are increasingly stringenter sweetener requirement, allow to add
Amount constantly reduces, so that more simple and quick, high sensitivity the detection method of exploitation necessitates.Therefore artificial synthesized sweet tea is studied
The detection method of taste agent is not only the demand of technology development and the demand of practical application.
Summary of the invention
The purpose of the present invention is to provide a kind of detection methods for neotame content in beverage, to solve in the prior art
Caused above-mentioned defect.
A kind of detection method for neotame content in beverage, includes the following steps：
(1) sample treatment liquid is prepared：Beverage 50ml is accurately weighed, weighed beverage is put into beaker and records beverage
With the total weight of beaker, after beaker is placed on the carbon dioxide and alcohol removed in beverage under 100 DEG C of water-baths, taking-up is cooled to
Room temperature adds original total weight with ultrapure water；
(2) titer is prepared：Prepare the neotame standard solution of various concentration；
(3) it is detected using the neotame standard solution that liquid chromatogram prepares step (2), it is bent to draw neotame concentration peak face
Line, using the concentration of neotame as abscissa, peak area is ordinate；
(4) the obtained sample treatment liquid of step (1) is examined under liquid phase chromatogram condition identical with step (3)
It surveys, the peak area of neotame is calculated, the dense of neotame in sample treatment liquid is calculated according to the standard curve that step (3) are established
Degree, and in conjunction with the volume and example weight of the sample treatment liquid, the content of neotame in beverage is calculated, formulas for calculating is such as
Under：
(wherein AsampleFor the peak area of sample treatment liquid, mstandardFor the peak area of titer, AStandardAt sample
Manage the amount of the substance of liquid, msampieFor the amount of the substance of titer, F is neotame standard solution purity).
Preferably, the condition of chromatography employed in the step (3) is,
Chromatographic column：C18 (25cm long, 4.6mm column diameter, 5 μm of filler particle size)；
Mobile phase：35% acetonitrile and 65% buffer (ammonium acetate)；
Mobile phase PH3.4-4.1
Detection wavelength：210nm；
Use second eyeball and water respectively, methanol and buffer solution, second eyeball and buffer solution do mobile phase, discovery using second eyeball and
Water makees mobile phase, and peak hangover is than more serious, and wider using methanol and buffer solution peak type, preferably second eyeball and buffer solution, which are done, flows
Phase, peak type are preferable.
Compared using different ratio second eyeball and buffer solution, 45% acetonitrile and 55% buffer, 30% second eyeball and 70% are slow
Solution, 20% second eyeball and 80% buffer solution, different proportion, appearance time and peak type is rushed to change.When measuring sample, discovery
30% second eyeball and 70% buffer solution, 20% second eyeball and 80% buffer solution this two ratios make mobile phase, target peak and miscellaneous peak
Separate best, and when making mobile phase using 30% second eyeball and 70% buffer solution, appearance time is more early, and peak shape is preferable, determines choosing
Mobile phase is done with 30% second eyeball and 70% buffer solution.
Preferably, be in step (2) prepare neotame standard solution concentration be 2mg/L, 10mg/L, 50mg/L,
100mg/L、200mg/L。
Preferably, first sodium heptanesulfonate is dissolved in secondary distilled water during the buffer, phosphoric acid is added, then
It is adjusted to pH=3.5 with triethylamine, acetonitrile is added and adjusts pH value of solution to 3.4~4.1 with phosphoric acid.
Preferably, it is room temperature that column temperature is controlled during liquid chromatographic detection, and flow rate of mobile phase is 0.7~1.7mL/min, into
Sample amount is 18~28 μ L.
Preferably, institute's beverage is sweet drink.
The advantage of the invention is that：Using this method detection neotame sample content, stabilization easy to operate, precision,
The needs that analysis can be fully met in terms of accuracy provide a kind of fast and accurately analysis method；Using 30% acetonitrile and
70% buffer solution does mobile phase, its peak area of neotame sample is good with concentration linear relationship, is easy to detect；Accurate good color
Spectral condition is suitble to the detection of most beverages in the market.
Detailed description of the invention
Fig. 1 is neotame canonical plotting of the present invention.
Specific embodiment
To be easy to understand the technical means, the creative features, the aims and the efficiencies achieved by the present invention, below with reference to
Specific embodiment, the present invention is further explained.
As shown in Fig. 1 figure, a kind of detection method for neotame content in beverage includes the following steps：
(1) sample treatment liquid is prepared：Beverage 50ml is accurately weighed, weighed beverage is put into beaker and records beverage
With the total weight of beaker, after beaker is placed on the carbon dioxide and alcohol removed in beverage under 100 DEG C of water-baths, taking-up is cooled to
Room temperature adds original total weight with ultrapure water；
(2) titer is prepared：Prepare the neotame standard solution of various concentration；
(3) it is detected using the neotame standard solution that liquid chromatogram prepares step (2), it is bent to draw neotame concentration peak face
Line, using the concentration of neotame as abscissa, peak area is ordinate；
(4) the obtained sample treatment liquid of step (1) is examined under liquid phase chromatogram condition identical with step (3)
It surveys, the peak area of neotame is calculated, the dense of neotame in sample treatment liquid is calculated according to the standard curve that step (3) are established
Degree, and in conjunction with the volume and example weight of the sample treatment liquid, the content of neotame in beverage is calculated, formulas for calculating is such as
Under：
(wherein AsampleFor the peak area of sample treatment liquid, mstandardFor the peak area of titer, AStandardAt sample
Manage the amount of the substance of liquid, msampieFor the amount of the substance of titer, F is neotame standard solution purity).
In the present embodiment, the condition of chromatography employed in the step (3) is,
Chromatographic column：C18 (25cm long, 4.6mm column diameter, 5 μm of filler particle size)；
Mobile phase：35% acetonitrile and 65% buffer (ammonium acetate)；
Mobile phase PH3.4-4.1
Detection wavelength：210nm；
In the present embodiment, the concentration in step (2) being the neotame standard solution prepared is 2mg/L, 10mg/L, 50mg/
L、100mg/L、200mg/L。
In the present embodiment, first sodium heptanesulfonate is dissolved in secondary distilled water during the buffer, is added
Phosphoric acid, then be adjusted to pH=3.5 with triethylamine, acetonitrile is added and adjusts pH value of solution to 3.4~4.1 with phosphoric acid.
In the present embodiment, it is room temperature that column temperature is controlled during liquid chromatographic detection, and flow rate of mobile phase is 0.7~1.7mL/
Min, sample volume are 18~28 μ L.
In the present embodiment, institute's beverage is sweet drink.
Testing principle of the present invention：The neotame standard solution for configuring various concentration is set with liquid chromatograph in the present invention
Neotame content in the standard solution of each concentration is measured under chromatographic condition, the peak area of neotame appearance is obtained, establishes concentration
Peak area standard curve, the test sample treatment fluid under identical liquid phase chromatogram condition, according to standard curve and in conjunction with the sample
The volume and example weight of product treatment fluid, calculate the content of neotame in beverage.
Use second eyeball and water respectively, methanol and buffer solution, second eyeball and buffer solution do mobile phase, discovery using second eyeball and
Water makees mobile phase, and peak hangover is than more serious, and wider using methanol and buffer solution peak type, preferably second eyeball and buffer solution, which are done, flows
Phase, peak type are preferable.
Compared using different ratio second eyeball and buffer solution, 45% acetonitrile and 55% buffer, 30% second eyeball and 70% are slow
Solution, 20% second eyeball and 80% buffer solution, different proportion, appearance time and peak type is rushed to change.When measuring sample, discovery
30% second eyeball and 70% buffer solution, 20% second eyeball and 80% buffer solution this two ratios make mobile phase, target peak and miscellaneous peak
Separate best, and when making mobile phase using 30% second eyeball and 70% buffer solution, appearance time is more early, and peak shape is preferable, determines choosing
Mobile phase is done with 30% second eyeball and 70% buffer solution.
Specific experiment part：
1, reagent：
Acetonitrile：Chromatographically pure；Sodium heptanesulfonate：Chromatographically pure；Triethylamine：Chromatographically pure；Water：Level-one water in GB/T6682-2008；
Phosphoric acid：It analyzes pure；Neotame standard items.
2, key instrument equipment：
High performance liquid chromatograph：With UV detector；Assay balance：Precision is 0.0001g.
3, chromatographic condition：
3.1 chromatographic column：C18 chromatographic column, 250mm (length) × 4.6mm (internal diameter), 5 μm of granularity.
3.2 buffer：First sodium heptanesulfonate is dissolved in secondary distilled water during buffer, phosphoric acid is added, then
It is adjusted to pH=3.5 with triethylamine, acetonitrile is added and adjusts pH value of solution to 3.4~4.1 with phosphoric acid.
3.3 column temperature：Room temperature.
3.4 flow rate of mobile phase：0.7~1.7mL/min.
3.5 sample volume：20μL.
3.6 Detection wavelength：210nm.
4, analytical procedure：
4.1 standard reserving solutions are prepared：
0.1000g neotame standard items are accurately weighed in 100mL volumetric flask, water is added to be settled to scale, this solution neotame is dense
Degree is 1.0mg/mL.
4.2 titers are prepared：
Pipette 0.020 respectively, 0.10,0.50,1.0,2.0mL stock solution into 10mL volumetric flask, be settled to scale with water,
The series neotame concentration respectively is 2mg/L, 10mg/L, 50mg/L, 100mg/L, 200mg/L.
The drafting of 4.3 standard curves：
Neotame standard solution detect using liquid chromatogram, the peak area of appearance is calculated, with standard solution
Concentration is abscissa, and corresponding peak area is that ordinate does standard curve, Specification Curve of Increasing such as Fig. 1,
The preparation of 4.4 sample treatment liquids：
Prepare sample treatment liquid：Accurately weigh beverage 50ml, weighed beverage is put into beaker and record beverage and
The total weight of beaker, after beaker is placed on the carbon dioxide and alcohol removed in beverage under 100 DEG C of water-baths, taking-up is cooled to room
Temperature adds original total weight with ultrapure water,
The detection of 4.5 samples：
Sample treatment liquid is detected under liquid phase chromatogram condition identical with 4.4, the peak area of neotame is calculated,
The standard curve established according to 4.4 calculates the concentration of neotame in sample treatment liquid, and in conjunction with the volume of the sample treatment liquid
And example weight, the content of neotame in beverage is calculated, formulas for calculating is as follows：
(wherein AsampleFor the peak area of sample treatment liquid, mstandardFor the peak area of titer, AStandardAt sample
Manage the amount of the substance of liquid, msampieFor the amount of the substance of titer, F is neotame standard solution purity).
Calculated result is indicated to 2 significant digits；The measurement result independent parallel twice obtained under the conditions of repeatability takes
Average value obtains final detection result, and the relative standard deviation (RSD) of independent parallel measurement result is not more than 5% twice.
It is computed, the measurement result independent parallel twice of neotame content is respectively 0.976% He in the present embodiment sweetener
0.969%, the relative standard deviation (RSD) of independent parallel measurement result is not more than 5% twice, and collimation is good, and testing result is steady
It is fixed.
In the present invention, using the content of this method detection neotame sample, stabilization easy to operate, in precision, accuracy
Aspect can fully meet the needs of analysis, provide a kind of fast and accurately analysis method；It is slow using 30% acetonitrile and 70%
It rushes solution and does mobile phase, its peak area of neotame sample is good with concentration linear relationship, is easy to detect；Accurate good chromatostrip
Part is suitble to the detection of most beverages in the market.
As known by the technical knowledge, the present invention can pass through the embodiment party of other essence without departing from its spirit or essential feature
Case is realized.Therefore, embodiment disclosed above, in all respects are merely illustrative, not the only.Institute
Have within the scope of the present invention or is included in the invention in the change being equal in the scope of the present invention.
Claims (6)
1. a kind of detection method for neotame content in beverage, it is characterised in that：Include the following steps：
(1) sample treatment liquid is prepared：Beverage 50ml is accurately weighed, weighed beverage is put into beaker and records beverage and burning
The total weight of cup, after beaker is placed on the carbon dioxide and alcohol removed in beverage under 100 DEG C of water-baths, taking-up is cooled to room
Temperature adds original total weight with ultrapure water；
(2) titer is prepared：Prepare the neotame standard solution of various concentration；
(3) it is detected using the neotame standard solution that liquid chromatogram prepares step (2), draws neotame concentration peak surface curve,
Using the concentration of neotame as abscissa, peak area is ordinate；
(4) the obtained sample treatment liquid of step (1) is detected under liquid phase chromatogram condition identical with step (3), is counted
The peak area for obtaining neotame is calculated, the concentration of neotame in sample treatment liquid is calculated according to the standard curve that step (3) are established, and tie
The volume and example weight for closing the sample treatment liquid, calculate the content of neotame in beverage, and formulas for calculating is as follows：
Wherein AsampleFor the peak area of sample treatment liquid, mstandardFor the peak area of titer, AStandardFor sample treatment liquid
The amount of substance, msampieFor the amount of the substance of titer, F is neotame standard solution purity.
2. a kind of detection method for neotame content in beverage according to claim 1, it is characterised in that：The step
(3) condition of the chromatography employed in is,
Chromatographic column：C18 (25cm long, 4.6mm column diameter, 5 μm of filler particle size)；
Mobile phase：30% acetonitrile and 70% buffer (ammonium acetate)；
Mobile phase PH3.4-4.1；
Detection wavelength：210nm.
3. a kind of detection method for neotame content in beverage according to claim 1, it is characterised in that：Step (2)
In be the concentration of neotame standard solution prepared be 2mg/L, 10mg/L, 50mg/L, 100mg/L, 200mg/L.
4. a kind of detection method for neotame content in beverage according to claim 2, it is characterised in that：The buffer
First sodium heptanesulfonate is dissolved in secondary distilled water in process for preparation, phosphoric acid is added, then be adjusted to pH=3.5 with triethylamine, is added
Acetonitrile simultaneously adjusts pH value of solution to 3.4~4.1 with phosphoric acid.
5. a kind of detection method for neotame content in beverage according to claim 1, it is characterised in that：Liquid chromatogram inspection
Control column temperature is room temperature during surveying, and flow rate of mobile phase is 0.7~1.7mL/min, and sample volume is 18~28 μ L.
6. a kind of detection method for neotame content in beverage according to claim 1, it is characterised in that：The beverage is
Sweet drink.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101634648A (en) * | 2009-08-21 | 2010-01-27 | 江南大学 | Method for detecting six trace sweetening agents in white spirit by ultra-high pressure liquid chromatography and time-of-flight mass spectrometry |
| CN105628818A (en) * | 2015-12-31 | 2016-06-01 | 上海美农生物科技股份有限公司 | Method for simultaneously detecting six sweetening agents in feed additive with high performance liquid chromatograph |
| CN105651893A (en) * | 2016-02-26 | 2016-06-08 | 重庆民泰香料化工有限责任公司 | HPLC (high performance liquid chromatography) determination method for neotame content in feed additive |
| CN106324150A (en) * | 2016-11-25 | 2017-01-11 | 四川省食品药品检验检测院 | Method for determining eight sweetening agents in Baijiu by high-performance liquid chromatography-low-temperature evaporative light scattering detector method |
| CN108169343A (en) * | 2016-12-07 | 2018-06-15 | 内蒙古蒙牛乳业（集团）股份有限公司 | The detection method of neotame content in milk-contained drink |
| CN108169342A (en) * | 2016-12-07 | 2018-06-15 | 内蒙古蒙牛乳业（集团）股份有限公司 | The detection method of neotame content in milk-contained drink |
-
2018
- 2018-07-23 CN CN201810811908.9A patent/CN108918716A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101634648A (en) * | 2009-08-21 | 2010-01-27 | 江南大学 | Method for detecting six trace sweetening agents in white spirit by ultra-high pressure liquid chromatography and time-of-flight mass spectrometry |
| CN105628818A (en) * | 2015-12-31 | 2016-06-01 | 上海美农生物科技股份有限公司 | Method for simultaneously detecting six sweetening agents in feed additive with high performance liquid chromatograph |
| CN105651893A (en) * | 2016-02-26 | 2016-06-08 | 重庆民泰香料化工有限责任公司 | HPLC (high performance liquid chromatography) determination method for neotame content in feed additive |
| CN106324150A (en) * | 2016-11-25 | 2017-01-11 | 四川省食品药品检验检测院 | Method for determining eight sweetening agents in Baijiu by high-performance liquid chromatography-low-temperature evaporative light scattering detector method |
| CN108169343A (en) * | 2016-12-07 | 2018-06-15 | 内蒙古蒙牛乳业（集团）股份有限公司 | The detection method of neotame content in milk-contained drink |
| CN108169342A (en) * | 2016-12-07 | 2018-06-15 | 内蒙古蒙牛乳业（集团）股份有限公司 | The detection method of neotame content in milk-contained drink |
Non-Patent Citations (5)
| Title |
|---|
| ANURADHA KUMARI等: "Stability of aspartame and neotame in pasteurized and in-bottle sterilized flavoured milk", 《FOOD CHEMISTRY》 * |
| 周魁等: "高效液相色谱法测定纽甜的含量", 《广东化工》 * |
| 孙洁等: "高效液相色谱法测定白酒中纽甜含量的不确定度评定", 《酿酒科技》 * |
| 彭飞进: "高效液相色谱法测定白酒中纽甜含量", 《云南化工》 * |
| 邓虹: "纽甜的特性及稳定性研究", 《饲料博览》 * |
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