CN108918351A - Device based on particle in optical acquisition aerosol and realization Raman spectrum detection - Google Patents
Device based on particle in optical acquisition aerosol and realization Raman spectrum detection Download PDFInfo
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- CN108918351A CN108918351A CN201810453749.XA CN201810453749A CN108918351A CN 108918351 A CN108918351 A CN 108918351A CN 201810453749 A CN201810453749 A CN 201810453749A CN 108918351 A CN108918351 A CN 108918351A
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- 239000002245 particle Substances 0.000 title claims abstract description 87
- 239000000443 aerosol Substances 0.000 title claims abstract description 72
- 230000003287 optical effect Effects 0.000 title claims abstract description 55
- 238000001237 Raman spectrum Methods 0.000 title claims abstract description 30
- 238000001514 detection method Methods 0.000 title claims abstract description 25
- 230000007246 mechanism Effects 0.000 claims abstract description 31
- 238000001069 Raman spectroscopy Methods 0.000 claims abstract description 27
- 239000010453 quartz Substances 0.000 claims abstract description 23
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 23
- 238000001228 spectrum Methods 0.000 claims abstract description 10
- 239000002775 capsule Substances 0.000 claims abstract description 6
- 230000009471 action Effects 0.000 claims abstract description 4
- 230000000295 complement effect Effects 0.000 claims description 17
- 230000005284 excitation Effects 0.000 claims description 7
- 239000007787 solid Substances 0.000 claims description 6
- 238000012576 optical tweezer Methods 0.000 claims description 4
- 230000000694 effects Effects 0.000 claims description 3
- 239000007921 spray Substances 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 4
- 239000007789 gas Substances 0.000 description 4
- 230000003595 spectral effect Effects 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 239000003245 coal Substances 0.000 description 2
- 230000002538 fungal effect Effects 0.000 description 2
- 230000001902 propagating effect Effects 0.000 description 2
- 238000009738 saturating Methods 0.000 description 2
- 239000000779 smoke Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 101100117236 Drosophila melanogaster speck gene Proteins 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000006229 carbon black Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001506 fluorescence spectroscopy Methods 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000001524 infective effect Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011859 microparticle Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 238000010183 spectrum analysis Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/06—Investigating concentration of particle suspensions
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/06—Investigating concentration of particle suspensions
- G01N15/0606—Investigating concentration of particle suspensions by collecting particles on a support
-
- G01N15/075—
Abstract
The invention discloses a kind of based on particle in optical acquisition aerosol and realizes the device of Raman spectrum detection, including two lasers, hollow laser beam generation mechanism, aerosol particles optical acquisition mechanism and Raman spectrum testing agency, the laser that first laser device generates is divided into two bundles circular cone hollow laser beam by hollow laser beam generation mechanism, in the aerosol circulation road for the quartz cavity that two beams circular cone hollow laser beam obtained focuses on aerosol particles optical acquisition mechanism under the action of the aerosol particles optical acquisition mechanism, building captures trap by the capsule feature optical that two beam circular cone hollow beams are formed, realize the optical acquisition to aerosol particles；Raman spectrum testing agency is detected for realizing the real time spectrum to capture particle.The method can carry out stable optical acquisition and Raman spectrum detection to aerosol particles, and eliminate the fluorescence background in particle Raman signal, process quick nondestructive, the final identification realized to particle in aerosol.
Description
Technical field
The present invention relates to optical tweezer, spectrum analysis and micro particles analyte detection fields, more particularly to one kind is in optical acquisition gas
Particle and the device of realization Raman spectrum detection in colloidal sol.
Background technique
Aerosol is that a kind of liquid or solid particle are suspended in the suspension system formed in gas medium, wherein particle phase
Characteristic diameter size be generally 1nm to 100 μm.When the concentration of the particles such as coal smoke, infective bacterial in atmosphere is more than a certain
When threshold value, which will have vital influence to air quality, climate change, human health, therefore to atmosphere
The detection of particle has become important a part of environment measuring in aerosol.
To the substance characteristics of the available particle of the optical detection of aerosol particles, when the shape for combining other features such as particle
The more information of available aerosol particles when shape or surface texture, and the optical detective technology of aerosol particles has fastly
The advantages that fast, lossless, highly sensitive has been one of current main research tendency.Although the Raman scattering of particle and its elasticity dissipate
It penetrates or fluorescence is compared, contain material information more abundant, but the Raman scattering signal of the particle due to floating in air is too
It is weak, so that mainly using fluorescence spectroscopy technique to the detection for the particulate samples being suspended in the air at present.But work as excitation light source
In visible light wave range, such as 488nm, 633nm and 780nm, many biological particles (pollen, fungal spore etc.) will be in very big light
It composes and occurs one or more fluorescence packets in region (such as 400nm-800nm), this will make the fluorescence light of particulate samples in aerosol
Spectrum lacks corresponding key spectral signature, and then hinders to its correct identification.
Therefore, how effective Raman detection is carried out to the particle in aerosol and realizes that correct identify is a urgency to it
Problem to be solved.
Summary of the invention
Technical problem to be solved by the invention is to provide one kind effectively to carry out optical acquisition and Raman to particle
Detection, and realize the device identified aerosol particles.
In order to solve the above technical problems, the technical solution adopted by the present invention is that：One kind is based in optical acquisition aerosol
Particle and the device for realizing Raman spectrum detection, it is characterised in that including：Two lasers, hollow laser beam generation mechanism, gas
The launch wavelength of colloidal sol particulate optical capture mechanism and Raman spectrum testing agency, first laser device and second laser is not
Deng the laser that first laser device generates is divided into two bundles circular cone hollow laser beam by hollow laser beam generation mechanism, is obtained
Two beam circular cone hollow laser beams focus on aerosol particles optics under the action of the aerosol particles optical acquisition mechanism
In the aerosol circulation road of the quartz cavity of capture mechanism, building is captured by the capsule feature optical that two beam circular cone hollow beams are formed and is fallen into
Trap realizes the optical acquisition to the aerosol particles sprayed into through nozzle in aerosol particles optical acquisition mechanism；Second laser
After the laser of generation is used to carry out the photobleaching several seconds to capture particle, the fluorescence background in acquired Raman signal is removed；Raman
Spectral detection mechanism is used to be acquired the reflection Raman signal of capture particle, realizes that the real time spectrum to capture particle is examined
It surveys.
A further technical solution lies in：The excitation wavelength of the first laser device is 480nm-550nm, and power is
900mW-1400mW；The excitation wavelength of the second laser is 330nm-450nm, power 1mW-10mW.
A further technical solution lies in：The hollow laser beam generation mechanism includes the first reflecting mirror, first semi-transparent half
Anti- mirror, the first convex lens, the first complementary conical lens group, the second reflecting mirror, third reflecting mirror, the 4th reflecting mirror, the second convex lens
Mirror and the second complementary conical lens group；It is adjusted by the direction of propagation that first reflecting mirror generates laser to first laser device
It is whole, then by the first semi-transparent semi-reflecting lens above-mentioned laser is divided into two bundles the identical solid laser of energy, wherein beam of laser according to
Secondary that hollow laser beam I is generated after the first convex lens, the first complementary conical lens group, it is anti-that another beam of laser successively passes through second
Mirror, third reflecting mirror, the 4th reflecting mirror, the second convex lens and the second complementary conical lens group is penetrated to generate and hollow laser beam I
The identical hollow laser beam II of power.
A further technical solution lies in：Aerosol particles optical acquisition mechanism includes symmetrically arranged first micro-
Object lens, the second microcobjective and quartz cavity, first microcobjective is for converging hollow laser beam I, and described the
For two microcobjectives for converging to hollow laser beam II, hollow laser beam I and hollow laser beam II pass through two micro- objects
Circular cone hollow laser beam is generated after the effect of mirror, and is focused in the aerosol circulation road of quartz cavity, is constructed using optical tweezer principle
Aerosol is sprayed into quartz cavity by the nozzle on quartz cavity, falls into standard to aerosol particle by cryptomere optical acquisition trap out
In the trap got ready, capture can be realized.
A further technical solution lies in：The Raman spectrum testing agency include third microcobjective, the first dichroic mirror,
Notch filter, third convex lens, the 4th convex lens, long pass filter, CCD camera and spectrometer, particle in aerosol
Reflection signal is divided into two-way after passing sequentially through third microcobjective and dichroic mirror, and the signal of reflection all the way therein is successively filtered through trap
CCD camera is arrived at after wave device, the 4th convex lens, the acquisition to the reflection signal of particle in aerosol is realized, by CCD camera to gas
Particle carries out real time imagery in colloidal sol；Second tunnel reflection signal arrives at spectrometer after third convex lens and long pass filter.
A further technical solution lies in：The focal length of two circular cone hollow laser beams is 0-1000 μm adjustable, and two circles
The direction of propagation of hollow laser beam is bored from the horizontal by 0-15 ° of angle.
A further technical solution lies in：The third microcobjective is 20 x Microscope Objectives.
A further technical solution lies in：First microcobjective and the second microcobjective are 50 x Microscope Objectives.
Generated beneficial effect is by adopting the above technical scheme：Described device effectively can carry out optics to particle
Capture and Raman detection, inhibit the fluorescence that it is generated by Photobleaching, and by dichroic mirror and filter to Raman
Rayleigh scattering in signal is eliminated, and be can get the Raman spectrum of the unstressed configuration background of sample A whithin a period of time, is passed through sample
The Spectra peak recognition of the Raman spectrum of product A realizes the identification to aerosol particles.
Detailed description of the invention
The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.
Fig. 1 is the functional block diagram of described device of the embodiment of the present invention；
Fig. 2 is the light path schematic diagram in hollow laser beam of embodiment of the present invention generation structure；
Fig. 3 is the operation schematic diagram of aerosol particles optical acquisition structure in the embodiment of the present invention；
Wherein：1, first laser device 2, second laser 3, the first reflecting mirror 4, the first semi-transparent semi-reflecting lens 5, the first convex lens
6, the first complementary conical lens group 7, the first microcobjective 8, quartz cavity 9, nozzle 10, third microcobjective 11, the first dichroic mirror
12, third convex lens 13, long pass filter 14, CCD camera 15, the 4th convex lens 16, notch filter 17, spectrometer 18,
Saturating 23, the second micro- object of two-mirror 19, third reflecting mirror 20, the 4th reflecting mirror 21, the second convex lens 22, the second complementary conical
Mirror.
Specific embodiment
With reference to the attached drawing in the embodiment of the present invention, technical solution in the embodiment of the present invention carries out clear, complete
Ground description, it is clear that described embodiment is only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
In the following description, numerous specific details are set forth in order to facilitate a full understanding of the present invention, but the present invention can be with
Implemented using other than the one described here other way, those skilled in the art can be without prejudice to intension of the present invention
In the case of do similar popularization, therefore the present invention is not limited by the specific embodiments disclosed below.
As shown in Figure 1, the embodiment of the invention discloses one kind based on particle in optical acquisition aerosol and to realize Raman light
The device of detection is composed, including：Two lasers, hollow laser beam generation mechanism, aerosol particles optical acquisition mechanism and drawing
Graceful spectral detection mechanism.First laser device 1 and the launch wavelength of second laser 2 differ, and pass through hollow laser beam generation mechanism
The laser that first laser device 1 generates is divided into two bundles circular cone hollow laser beam, two beams circular cone hollow laser beam obtained is in institute
State the aerosol that the quartz cavity 8 of aerosol particles optical acquisition mechanism is focused under the action of aerosol particles optical acquisition mechanism
In circulation road, building captures trap by the capsule feature optical that two beam circular cone hollow beams are formed, and realizes to through aerosol particles light
Learn the optical acquisition for the aerosol particles that nozzle 9 sprays into capture mechanism；The laser that second laser 2 generates is used for micro- to capturing
After grain carries out the photobleaching several seconds, the fluorescence background in acquired Raman signal is removed；Raman spectrum testing agency is used for capture
The reflection Raman signal of particle is acquired, and realizes that the real time spectrum to capture particle detects.
Further, first laser device 1 generates laser, and designs for medium wavelength, due to its meeting so that sample generates fluorescence
Interference, so the laser irradiation generated using second laser 2 on sample, is generated reflection Raman signal and utilizes photobleaching original
Reason removal fluorescence background.Preferably, the excitation wavelength of the first laser device 1 is 480nm-550nm, power 900mW-
1400mW；The excitation wavelength of the second laser 2 is 330nm-450nm, power 1mW-10mW.
Further, as shown in Figure 1, the hollow laser beam generation mechanism includes that the first reflecting mirror 3, first is semi-transparent semi-reflecting
Mirror 4, the first convex lens 5, the first complementary conical lens group 6, the second reflecting mirror 18, third reflecting mirror 19, the 4th reflecting mirror 20,
Two convex lenses 21 and the second complementary conical lens group 22；The biography of laser is generated to first laser device 1 by first reflecting mirror 3
It broadcasts direction to be adjusted, above-mentioned laser is then divided into two bundles the identical solid laser of energy by the first semi-transparent semi-reflecting lens 4,
Middle beam of laser successively generates hollow laser beam I, another beam of laser after the first convex lens 5, the first complementary conical lens group 6
It is successively saturating by the second reflecting mirror 18, third reflecting mirror 19, the 4th reflecting mirror 20, the second convex lens 21 and the second complementary conical
Microscope group 22 generates hollow laser beam II identical with I power of hollow laser beam, and hollow laser beam generates optical path such as Fig. 2 in structure
It is shown.
Further, as shown in Figure 1, the laser that the first laser device 1 initially generates passes through 3 rear direction of the first reflecting mirror
Become upwardly propagating for 0-15 ° with horizontal direction angle, it is identical solid to be divided into two beam energies after the first semi-transparent semi-reflecting lens 4
Laser beam, the hollow laser beam form circular cone hollow laser beam after passing through a microcobjective respectively and converge at quartz cavity
In aerosol circulation road, two circular cone hollow laser beams are crossed to form light beam capsule structure to constitute optical acquisition trap, circular cone top
30-60 ° of angle is adjustable；By changing the distance in hollow laser beam generation structure in two complementary conical lens groups between Conical Lenses,
The focus and maximum gauge of i.e. changeable obtained circular cone hollow laser beam, maximum gauge 10mm-20mm is adjustable, hollow beam two
0-1000 μm of focal length adjustable.The hollow laser beam generates the hollow laser direction of structure output from the horizontal by 0-
15 ° of angles upwardly propagate, and it is 450mW-700mW that the power of two bundle of hollow laser is identical.
As shown in figures 1 and 3, aerosol particles optical acquisition mechanism includes symmetrically arranged first microcobjective 7
And second microcobjective 23 and the quartz cavity 8 between two microcobjectives.First microcobjective 7 is used for hollow
Laser beam I is converged, and second microcobjective 23 is for converging hollow laser beam II, and hollow laser beam I is in
Circular cone hollow laser beam is generated after the effect that empty laser beam II passes through two microcobjectives, and focuses on the aerosol of quartz cavity 8
In circulation road, cryptomere optical acquisition trap is constructed using optical tweezer principle, is sprayed into aerosol by the nozzle 9 on quartz cavity 8
It in quartz cavity 8, is fallen into ready trap to aerosol particle, capture can be realized.
In the aerosol particles optical acquisition structure, the light beam capsule structure that two circular cone hollow laser beams are formed be may be implemented
Capture to sample A, from the horizontal by 5 ° of -15 ° of angles, the building upwardly propagated can effectively cancel out larger micro- in direction
The gravity of grain (50 μm -100 μm) guarantees the stability of capture；In addition to this, optical acquisition can make sample A from quartz cavity
In other suspended particles optical interference and chemical interaction, and be optically trapped in air in particle and in outstanding
Floating state, can increase the contact area of laser and particle, avoid particle by heat of solution or damage.
Further, as shown in Figure 1, the Raman spectrum testing agency includes third microcobjective 10, the first dichroic mirror
11, notch filter 16, third convex lens 12, the 4th convex lens 15, long pass filter 13, CCD camera 14 and spectrometer 17.
The reflection signal of particle is divided into two-way after passing sequentially through third microcobjective 10 and dichroic mirror 11 in aerosol, therein anti-all the way
It penetrates signal and successively arrives at reflection of the realization of CCD camera 14 to particle in aerosol after notch filter 16, the 4th convex lens 15
The acquisition of signal carries out real time imagery to particle in aerosol by CCD camera 14；Signal is reflected through third convex lens 12 in second tunnel
With arrival spectrometer 17 after long pass filter 13.
The laser that the second laser 2 generates is projected directly on sample A, photobleaching is carried out to it and generates reflection drawing
Graceful signal, the reflection Raman signal are filtered after being collected by third microcobjective 23 by the first dichroic mirror 11, and a part is successively
It is acquired after notch filter and convex lens by CCD camera, another part passes sequentially through convex lens and long pass filter is laggard
Enter spectrometer.
Third microcobjective preferentially uses 20 x Microscope Objectives 10, for the acquisition of optical signalling, by reflection signal and dissipates
Signal is penetrated to be transmitted in subsequent CCD camera 14 and spectrometer 17.First dichroic mirror 11 can with being applied in combination for notch filter 16
With filter out acquisition signal in reflection signal, guarantee enter CCD camera 14 in signal it is pure, CCD camera 14 be used for sample
Product particle real time imagery.The Raman that can be filtered out in acquisition signal is applied in combination with long pass filter 13 in first dichroic mirror 11
Signal removes Rayleigh scattering.Since Rayleigh intensity is much larger than Raman scattering, if not removing Rayleigh scattering, Raman scattering
Signal will be submerged, and can not be effectively detected.
A specific embodiment of the invention is as follows：
1 generation wavelength of first laser device is the laser of 488nm, power 1000mW, after which passes through the first reflecting mirror 3
Direction becomes upwardly propagating with 10 ° of horizontal direction angle, then passes through that be divided into two beam energies after the first semi-transparent semi-reflecting lens 4 identical
Solid laser beam, wherein beam of laser successively after the first convex lens 5 and the first complementary conical lens group 6 generate diameter be
The hollow laser beam I of 15mm, another beam of laser successively pass through the second reflecting mirror 18, third reflecting mirror 19, the 4th reflecting mirror 20,
It is the hollow of 15mm that diameter identical with I energy of hollow laser beam is generated after two convex lenses 21 and the second complementary conical lens group 22
The power of laser beam II, two bundle of hollow laser beams is 500mW.
It is preferable to use 50 x Microscope Objectives, two 50 x Microscope Objectives point for first microcobjective 7 and the second microcobjective 23
Two bundle of hollow laser are not focused in quartz cavity 8, constitute optical acquisition trap.Second laser II is opened, is generated
The laser of 405nm is transmitted in the optical trap of two hollow laser beams formation.
The Raman spectrum for acquiring air in quartz cavity 8 carries out spectroscopic calibration, and 17 acquisition time of spectrometer is set as 3min, passes through
Calibration to oxygen and nitrogen Raman shift in quartz cavity 8 realizes that the wave number accuracy of spectrometer 17 is ± 5cm-1。
Aerosol is sprayed into quartz cavity 8 by nozzle 9, is realized by ready optical acquisition device to particulate samples A's
Stablize capture.
After the reflection signal of sample A passes sequentially through third microcobjective 10, the first dichroic mirror 11 and notch filter 16, warp
CCD camera 14 is arrived at after the convergence of 4th convex lens 15, the acquisition of the reflection signal to sample A is realized, by CCD camera 14 to sample
A particle carries out real time imagery, to observe the pattern variation of sample A under laser irradiation；It is aobvious that Raman scattering signal passes sequentially through third
Speck mirror 10, the first dichroic mirror 11 arrive at spectrometer 17 after third convex lens 12 and long pass filter 13.When spectrometer 17 acquires
Between 0.1s, acquisition interval 0.4s, altogether acquire 16 spectrum, acquire wave band 400cm-1-3300cm-1；What second laser 2 generated
Laser constantly carries out photobleaching to sample A, so that the fluorescence interference that collected Raman spectrum is subject to constantly reduces, spectral quality
It becomes better and better.After starting acquisition signal 8 seconds, the high quality Raman spectrum of the removal fluorescence background of sample A can be obtained.
By carrying out Spectra peak recognition to collected spectrum, may be implemented to fungal spore (raman characteristic peak in aerosol：
2183cm-1、2948cm-1), pollen (raman characteristic peak：1600cm-1、2948cm-1、2970cm-1、3010cm-1), carbon black (Raman
Characteristic peak：1367cm-1、2714cm-1), coal smoke (raman characteristic peak：1590cm-1、1360cm-1) etc. particles identification.
Described device effectively can carry out optical acquisition and Raman detection to particle, be generated by Photobleaching to it
Fluorescence is inhibited, and is eliminated by dichroic mirror and filter to the Rayleigh scattering in Raman signal, whithin a period of time
The Raman spectrum that can get the unstressed configuration background of sample A is realized by the Spectra peak recognition of the Raman spectrum of sample A to aerosol
The identification of particle.
Claims (8)
1. a kind of device based on particle in optical acquisition aerosol and realization Raman spectrum detection, it is characterised in that including：Two
A laser, hollow laser beam generation mechanism, aerosol particles optical acquisition mechanism and Raman spectrum testing agency, first swashs
The launch wavelength of light device (1) and second laser (2) differs, and is produced first laser device (1) by hollow laser beam generation mechanism
Raw laser is divided into two bundles circular cone hollow laser beam, and two beams circular cone hollow laser beam obtained is in the aerosol particles optics
It focuses in the aerosol circulation road of quartz cavity (8) of aerosol particles optical acquisition mechanism, constructs under the action of capture mechanism
Trap is captured by the capsule feature optical that two beam circular cone hollow beams are formed, is realized to through being sprayed in aerosol particles optical acquisition mechanism
The optical acquisition for the aerosol particles that mouth (9) sprays into；The laser that second laser (2) generates is used to carry out light drift to capture particle
After the white several seconds, the fluorescence background in acquired Raman signal is removed；Raman spectrum testing agency is used for the reflection to capture particle
Raman signal is acquired, and realizes that the real time spectrum to capture particle detects.
2. the device based on particle in optical acquisition aerosol and realization Raman spectrum detection as described in claim 1, special
Sign is：The excitation wavelength of the first laser device (1) is 480nm-550nm, power 900mW-1400mW；Described second swashs
The excitation wavelength of light device (2) is 330nm-450nm, power 1mW-10mW.
3. the device based on particle in optical acquisition aerosol and realization Raman spectrum detection as described in claim 1, special
Sign is：The hollow laser beam generation mechanism includes the first reflecting mirror (3), the first semi-transparent semi-reflecting lens (4), the first convex lens
(5), the first complementary conical lens group (6), the second reflecting mirror (18), third reflecting mirror (19), the 4th reflecting mirror (20), second convex
Lens (21) and the second complementary conical lens group (22)；Laser is generated to first laser device (1) by first reflecting mirror (3)
The direction of propagation be adjusted, it is identical solid that above-mentioned laser is then divided into two bundles energy by the first semi-transparent semi-reflecting lens (4)
Laser, wherein beam of laser successively generates hollow laser beam after the first convex lens (5), the first complementary conical lens group (6)
I, another beam of laser successively passes through the second reflecting mirror (18), third reflecting mirror (19), the 4th reflecting mirror (20), the second convex lens
(21) and the second complementary conical lens group (22) generates hollow laser beam II identical with I power of hollow laser beam.
4. the device based on particle in optical acquisition aerosol and realization Raman spectrum detection as claimed in claim 3, special
Sign is：Aerosol particles optical acquisition mechanism includes symmetrically arranged first microcobjective (7) and the second microcobjective
(23) quartz cavity (8) and between two microcobjectives, first microcobjective (7) are used for hollow laser beam I
It is converged, second microcobjective (23) is for converging hollow laser beam II, hollow laser beam I and hollow laser
Circular cone hollow laser beam is generated after the effect that beam II passes through two microcobjectives, and focuses on the aerosol circulation of quartz cavity (8)
In road, cryptomere optical acquisition trap is constructed using optical tweezer principle, is sprayed into aerosol by the nozzle (9) on quartz cavity (8)
It in quartz cavity (8), is fallen into ready trap to aerosol particle, capture can be realized.
5. the device based on particle in optical acquisition aerosol and realization Raman spectrum detection as claimed in claim 4, special
Sign is：The Raman spectrum testing agency includes third microcobjective (10), the first dichroic mirror (11), notch filter
(16), third convex lens (12), the 4th convex lens (15), long pass filter (13), CCD camera (14) and spectrometer (17),
The reflection signal of particle, which passes sequentially through, in aerosol is divided into two-way after third microcobjective (10) and dichroic mirror (11), and therein one
Road reflects signal and successively arrives at CCD camera (14) after notch filter (16), the 4th convex lens (15), realizes in aerosol
The acquisition of the reflection signal of particle carries out real time imagery to particle in aerosol by CCD camera (14)；Signal warp is reflected on second tunnel
Third convex lens (12) and long pass filter (13) arrive at spectrometer (17) afterwards.
6. the device based on particle in optical acquisition aerosol and realization Raman spectrum detection as claimed in claim 4, special
Sign is：The focal length of two circular cone hollow laser beams is 0-1000 μm adjustable, and the direction of propagation of two circular cone hollow laser beams
From the horizontal by 0-15 ° of angle.
7. the device based on particle in optical acquisition aerosol and realization Raman spectrum detection as claimed in claim 4, special
Sign is：The third microcobjective (10) is 20 x Microscope Objectives.
8. the device based on particle in optical acquisition aerosol and realization Raman spectrum detection as claimed in claim 4, special
Sign is：First microcobjective (7) and the second microcobjective (23) are 50 x Microscope Objectives.
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|---|---|---|---|---|
| CN109613708A (en) * | 2019-01-16 | 2019-04-12 | 长春理工大学 | A kind of hollow four trap system of local based on dual beam configuration |
| CN109870394A (en) * | 2018-12-30 | 2019-06-11 | 江苏苏净集团有限公司 | A kind of dual-beam aerosol particle concentration detection device |
| CN112014260A (en) * | 2020-08-08 | 2020-12-01 | 之江实验室 | Method and device for rapidly detecting microorganisms by capturing particles through optical trap |
| CN113310860A (en) * | 2021-05-28 | 2021-08-27 | 中国矿业大学 | Aerosol particle rapid detection device and method based on SIBS and Raman spectrum |
| CN113358535A (en) * | 2021-05-31 | 2021-09-07 | 中国矿业大学 | Free silicon dioxide dust concentration online continuous detection device based on Raman spectrum |
| CN113820301A (en) * | 2021-11-25 | 2021-12-21 | 之江实验室 | Method and device for identifying microorganism species by using Raman optical tweezers |
| CN114088478A (en) * | 2022-01-24 | 2022-02-25 | 之江实验室 | Method and device for capturing aerosol by using suspended optical tweezers |
| CN114383984A (en) * | 2021-12-06 | 2022-04-22 | 浙江大学 | System for capturing particulate matter and measuring phase state, morphology and chemical components of particulate matter |
| CN114577681A (en) * | 2022-05-07 | 2022-06-03 | 之江实验室 | Aerosol weak Raman spectrum signal detection device and application method thereof |
| US20220238246A1 (en) * | 2021-01-26 | 2022-07-28 | Worcester Polytechnic Institute | Fiber optical tweezers |
| CN115112632A (en) * | 2022-07-11 | 2022-09-27 | 浙江大学 | Aerosol chemical reaction device based on double-camera multi-optical-trap optical tweezers |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101149449A (en) * | 2007-10-26 | 2008-03-26 | 哈尔滨工程大学 | Double core single optical fiber optical tweezers for capturing minute particle and its manufacture method |
| WO2009106348A2 (en) * | 2008-02-27 | 2009-09-03 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Optical trapping particle and optical trapping method |
| WO2010010121A1 (en) * | 2008-07-22 | 2010-01-28 | Centre National De La Recherche Scientifique (Cnrs) | Method for reducing interference and crosstalk in double optical tweezers using a single laser source, and apparatus using the same |
| CN104216103A (en) * | 2014-09-05 | 2014-12-17 | 华中科技大学 | Micropipe and optical tweezers measurement and control system |
-
2018
- 2018-05-14 CN CN201810453749.XA patent/CN108918351A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101149449A (en) * | 2007-10-26 | 2008-03-26 | 哈尔滨工程大学 | Double core single optical fiber optical tweezers for capturing minute particle and its manufacture method |
| WO2009106348A2 (en) * | 2008-02-27 | 2009-09-03 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Optical trapping particle and optical trapping method |
| WO2010010121A1 (en) * | 2008-07-22 | 2010-01-28 | Centre National De La Recherche Scientifique (Cnrs) | Method for reducing interference and crosstalk in double optical tweezers using a single laser source, and apparatus using the same |
| CN104216103A (en) * | 2014-09-05 | 2014-12-17 | 华中科技大学 | Micropipe and optical tweezers measurement and control system |
Non-Patent Citations (2)
| Title |
|---|
| 胡孔云 等: "采用超连续谱激光的双光束光纤光阱实验", 《中国光学》 * |
| 蔡宸 等: "光镊技术在气溶胶物理化学表征中的应用", 《中国光学》 * |
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109870394A (en) * | 2018-12-30 | 2019-06-11 | 江苏苏净集团有限公司 | A kind of dual-beam aerosol particle concentration detection device |
| CN109870394B (en) * | 2018-12-30 | 2023-10-10 | 江苏苏净集团有限公司 | Double-beam aerosol particle concentration detection device |
| CN109613708B (en) * | 2019-01-16 | 2020-12-11 | 长春理工大学 | Local hollow four-trap system based on double-beam structure |
| CN109613708A (en) * | 2019-01-16 | 2019-04-12 | 长春理工大学 | A kind of hollow four trap system of local based on dual beam configuration |
| CN112014260A (en) * | 2020-08-08 | 2020-12-01 | 之江实验室 | Method and device for rapidly detecting microorganisms by capturing particles through optical trap |
| US20220238246A1 (en) * | 2021-01-26 | 2022-07-28 | Worcester Polytechnic Institute | Fiber optical tweezers |
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| CN113310860A (en) * | 2021-05-28 | 2021-08-27 | 中国矿业大学 | Aerosol particle rapid detection device and method based on SIBS and Raman spectrum |
| CN113358535A (en) * | 2021-05-31 | 2021-09-07 | 中国矿业大学 | Free silicon dioxide dust concentration online continuous detection device based on Raman spectrum |
| CN113820301A (en) * | 2021-11-25 | 2021-12-21 | 之江实验室 | Method and device for identifying microorganism species by using Raman optical tweezers |
| CN114383984A (en) * | 2021-12-06 | 2022-04-22 | 浙江大学 | System for capturing particulate matter and measuring phase state, morphology and chemical components of particulate matter |
| CN114088478A (en) * | 2022-01-24 | 2022-02-25 | 之江实验室 | Method and device for capturing aerosol by using suspended optical tweezers |
| CN114577681A (en) * | 2022-05-07 | 2022-06-03 | 之江实验室 | Aerosol weak Raman spectrum signal detection device and application method thereof |
| CN115112632A (en) * | 2022-07-11 | 2022-09-27 | 浙江大学 | Aerosol chemical reaction device based on double-camera multi-optical-trap optical tweezers |
| CN115112632B (en) * | 2022-07-11 | 2023-06-02 | 浙江大学 | Aerosol chemical reaction device based on double-camera multi-optical-trap optical tweezers |
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