CN108913330B - Extraction method of wheat germ compound oil - Google Patents
Extraction method of wheat germ compound oil Download PDFInfo
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Abstract
The invention provides a method for extracting wheat germ compound oil, which comprises 7 steps of raw material crushing, microwave treatment, compound enzymolysis oil extraction, demulsification, oil cleaning, decoloration, deodorization and the like; the invention firstly adopts a high-temperature instant blasting method to blast the wheat germ and the sesame, can fully inactivate various enzymes in the wheat germ and the sesame, avoids the damage of high-temperature enzyme inactivation to nutrient components, and creates conditions for the subsequent extraction process; the microwave treatment process can crack and expand the cell walls of one part of the crushed wheat germ and sesame, so that part of fat is in a free state, and the extraction efficiency of the compound oil can be effectively improved; in addition, the invention combines the enzyme extraction-demulsification process, thereby effectively reducing the loss of oil and improving the extraction rate of oil; in addition, the invention adopts a salt washing-water washing process, so that impurities in the oil can be washed, and the obtained oil has high purity, good color and excellent quality.
Description
Technical Field
The invention belongs to the technical field of edible oil refining methods, and particularly relates to a method for extracting wheat germ compound oil.
Background
The wheat germ oil is grain oil prepared by taking wheat germs as a raw material source, is rich in various nutritional ingredients in wheat, and is always favored by people. The wheat germ oil contains rich beneficial components such as unsaturated fatty acid substances, natural vitamin E, sterol, octacosanol, cephalin, lecithin and the like, and is a nutritional health-care substance with extremely high nutritional value. The nutritional components and proportion in the wheat germ oil are in accordance with the human health standard, and a large amount of unsaturated fatty acid in the wheat germ oil can well protect the blood circulation system of a human body, and has good effects of treating cardiovascular and cerebrovascular diseases, preventing arteriosclerosis, and reducing the contents of blood sugar, blood pressure and cholesterol; wheat germ oil has been widely used in various industries such as food, medicine, cosmetics, etc.
At present, edible oil is mostly produced by adopting a squeezing method or a normal-pressure solvent extraction method, the yield of refined oil is low, the solvent residue is high, and the nutrient components in the oil product are seriously damaged at a high temperature, so that the edible oil product has low nutritional value, poor safety guarantee and no competitive advantage, and a large amount of raw material resources are wasted. Few enterprises with high strength adopt a CO2 supercritical extraction technology, so that oil products with good quality can be extracted, but equipment investment is large at one time, and because extraction is carried out under a high pressure state, the extraction volume is small (1000 liters at the maximum), the production capacity is limited, industrial large-scale production cannot be formed, the product cost is high, and the product has no market competitive advantage.
Therefore, the development of the wheat germ oil with low cost, feasible process, high extraction rate and good oil quality has very wide prospect.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides the method for extracting the wheat germ oil, and the process ensures that the extracted compound oil has high natural nutrient content, high oil product purity, less impurities, bright color and high extraction rate.
In order to solve the problems, the technical scheme adopted by the invention is as follows:
a method for extracting wheat germ compound oil comprises the following steps:
(1) crushing raw materials: filling clean wheat germs with good quality and cleaned sesame seeds subjected to cleaning and peeling into a crushing tank, maintaining the temperature at 130-160 ℃ and the pressure at 1.2-1.6MPa for 10-20min, and then instantaneously blasting to obtain crushed raw materials;
(2) microwave treatment: irradiating the raw material powder obtained in the step (1) for 60-120 seconds in a microwave environment of 900-1200 MHz;
(3) compound enzymolysis oil extraction: taking the raw material powder subjected to microwave treatment in the step (2), and mixing the raw material powder in mass ratio: adding water in a ratio of 1-2: 8-20, and then adding a complex enzyme of cellulase and protease for hydrolysis; after enzymolysis, carrying out centrifugal separation for 20-30min under the condition that the centrifugal speed is 5000-10000 r/min to obtain free germ oil, emulsion, enzymatic hydrolysate and residues;
(4) performing microwave treatment on the emulsion, wherein the microwave power is 400-500W, the microwave time is 10-30min, then adding ethanol, the concentration of the added ethanol is 60% -80%, and then performing centrifugal separation to obtain free germ oil;
(5) combining the free germ oil obtained in the steps (3) and (4), heating to 70-80 ℃, adding saline water at the temperature of 70-80 ℃, stirring for 15-20min under the condition of 50-60r/min, then adding hot water at the temperature of 70-80 ℃ into the oil material, stirring for 8-12min under the condition of 25-35r/min, standing for 2-3h, discharging water and precipitate, and obtaining the oil material after water washing;
(6) and (3) decoloring treatment: pumping the water-washed oil obtained in the step (5) into a decoloring tank under a vacuum condition, heating the oil to 80-90 ℃ while stirring, closing a heat conduction valve, and preserving heat for 10-30min by using waste heat; then absorbing the activated carbon into a decoloring tank, maintaining for 20-30min, and filtering after decoloring;
(7) deodorizing the oil material decolorized in the step (6) at the temperature of 160-;
(8) and cooling the deodorized oil to room temperature by cooling water circulation, and sieving with a 200-mesh and 400-mesh sieve to obtain the wheat germ composite oil.
Preferably, the weight ratio of the wheat germ to the sesame in the step (1) is 5-10: 1-2.
Preferably, the weight ratio of cellulase to protease in step (3) is 1: 4-6, and the using amount of the complex enzyme is 2-4% of the weight of the raw material powder.
Preferably, the protease in step (3) includes acid protease, papain, neutral protease and ALCALASE2.4L protease; the enzymolysis temperature is 40-60 ℃, and the enzymolysis time is 4-8 h.
Preferably, the ethanol is added in the step (4) in an amount of 0.2-0.4L/Kg based on the volume of ethanol/weight of the emulsion.
Preferably, the mass concentration of the saline water in the step (5) is 5-10%, and the addition amount of the saline water is 2-4% of the free germ oil.
In addition, the invention also claims wheat germ compound oil extracted by the extraction method.
Compared with the prior art, the invention has the technical effects that:
(1) the invention provides a method for extracting wheat germ compound oil, which comprises 7 steps of raw material crushing, microwave treatment, compound enzymolysis oil extraction, demulsification, oil cleaning, decoloration, deodorization and the like, wherein the 7 steps are taken as an integral process, so that the extracted compound oil has high natural nutrient content, high oil product purity, less impurities, bright color and high extraction rate;
(2) according to the invention, a certain amount of sesame is added into wheat germ for compounding, the nutrient components of the wheat germ are adjusted by utilizing the characteristic that the sesame oil extracted from the sesame is rich in fatty acid and linoleic acid, the obtained compound oil is reasonable in nutrient component collocation, the nutrient components of the wheat germ and the sesame oil are integrated, and the dietary requirements of Chinese people are met;
(3) the invention firstly adopts a high-temperature instant blasting method to blast the wheat germ and the sesame, can fully inactivate various enzymes in the wheat germ and the sesame, avoids the damage of high-temperature enzyme inactivation to nutrient components, and creates conditions for the subsequent extraction process; the microwave treatment process can crack and expand the cell walls of one part of the crushed wheat germ and sesame, so that part of fat is in a free state, and the extraction efficiency of the compound oil can be effectively improved; in addition, the invention combines the enzyme extraction-demulsification process, thereby effectively reducing the loss of oil and improving the extraction rate of oil; moreover, the invention adopts a salt washing-water washing process, so that impurities in the oil can be washed, and the obtained oil has high purity, good color and excellent quality;
(4) the invention has simple process flow, easy control of production operation and suitability for large-scale production, and 7 process steps complement each other and are matched with each other, thereby obtaining the wheat germ compound oil with high quality.
Detailed Description
The technical scheme of the invention is further explained by combining the embodiment as follows:
example 1
A method for extracting wheat germ compound oil comprises the following steps:
(1) crushing raw materials: filling cleaned and peeled semen Tritici Aestivi germ and semen Sesami with good quality into grinding tank, maintaining at 150 deg.C and 1.3MPa for 15min, and instantly blasting to obtain ground material; wherein the weight ratio of the wheat germ to the sesame is 6: 1;
(2) microwave treatment: irradiating the raw material powder obtained in the step (1) for 80 seconds in a microwave environment of 1000 MHz;
(3) compound enzymolysis oil extraction: taking the raw material powder subjected to microwave treatment in the step (2), and mixing the raw material powder in mass ratio: adding water according to the proportion of 1:10, and then adding a complex enzyme of cellulase, neutral protease and ALCALASE2.4L protease for hydrolysis, wherein the enzymolysis temperature is 45 ℃ and the enzymolysis time is 5 h; centrifuging at 8000r/min for 25min to obtain free embryo oil, emulsion, enzymolysis solution and residue; the weight ratio of the cellulase to the protease is 1: 5;
(4) performing microwave treatment on the emulsion, wherein the microwave power is 450W, the microwave time is 20min, then adding ethanol, the concentration of the added ethanol is 70%, and then performing centrifugal separation to obtain free germ oil, wherein the adding amount of the ethanol is 0.3L/Kg of the volume of the ethanol/the weight of the emulsion;
(5) combining the free germ oil obtained in the steps (3) and (4), heating to 75 ℃, adding saline water with the mass concentration of 6% and the temperature of 75 ℃, stirring for 15min under the condition of 55r/min, then adding hot water with the temperature of 75 ℃ into the oil material, stirring for 10min under the condition of 30r/min, standing for 2h, discharging water and precipitate, and obtaining the oil material after water washing; the addition amount of the saline water is 3% of the free germ oil;
(6) and (3) decoloring treatment: pumping the water-washed oil obtained in the step (5) into a decoloring tank under a vacuum condition, heating the oil to 85 ℃ while stirring, closing a heat conduction valve, and preserving the heat for 20min by using waste heat; then absorbing the activated carbon into a decoloring tank, maintaining for 25min, and filtering after decoloring;
(7) deodorizing the oil material decolorized in the step (6) at the temperature of 180 ℃ and the vacuum degree of 0.08 MPa;
(8) and cooling the deodorized oil to room temperature by circulating cooling water, and sieving by a 300-mesh sieve to obtain the wheat germ compound oil.
Example 2
A method for extracting wheat germ compound oil comprises the following steps:
(1) crushing raw materials: filling cleaned and peeled semen Tritici Aestivi germ and semen Sesami with good quality into grinding tank, maintaining at 1450 deg.C and 1.5MPa for 15min, and instantaneously blasting to obtain ground material; wherein the weight ratio of the wheat germ to the sesame is 8: 1.3;
(2) microwave treatment: irradiating the raw material powder obtained in the step (1) for 80 seconds in a 1100MHz microwave environment;
(3) compound enzymolysis oil extraction: taking the raw material powder subjected to microwave treatment in the step (2), and mixing the raw material powder in mass ratio: adding water according to the proportion of 1:9, and then adding a complex enzyme of cellulase, papain and ALCALASE2.4L protease for hydrolysis, wherein the enzymolysis temperature is 50 ℃, and the enzymolysis time is 5 hours; centrifuging at 8000r/min for 25min to obtain free embryo oil, emulsion, enzymolysis solution and residue; the weight ratio of the cellulase to the protease is 1: 6;
(4) performing microwave treatment on the emulsion, wherein the microwave power is 450W, the microwave time is 25min, then adding ethanol, the concentration of the added ethanol is 70%, and then performing centrifugal separation to obtain free germ oil, wherein the adding amount of the ethanol is 0.35L/Kg of the volume of the ethanol/the weight of the emulsion;
(5) combining the free germ oil obtained in the steps (3) and (4), heating to 75 ℃, adding saline water with the mass concentration of 7% and the temperature of 75 ℃, stirring for 15min under the condition of 55r/min, then adding hot water with the temperature of 75 ℃ into the oil material, stirring for 10min under the condition of 30r/min, standing for 2h, discharging water and precipitate, and obtaining the oil material after water washing; the addition amount of the saline water is 3% of the free germ oil;
(6) and (3) decoloring treatment: pumping the water-washed oil obtained in the step (5) into a decoloring tank under a vacuum condition, heating the oil to 85 ℃ while stirring, closing a heat conduction valve, and preserving the heat for 20min by using waste heat; then absorbing the activated carbon into a decoloring tank, maintaining for 25min, and filtering after decoloring;
(7) deodorizing the oil material decolorized in the step (6) at the temperature of 180 ℃ and the vacuum degree of 0.09 MPa;
(8) and cooling the deodorized oil to room temperature by circulating cooling water, and sieving by a 300-mesh sieve to obtain the wheat germ compound oil.
Example 3
A method for extracting wheat germ compound oil comprises the following steps:
(1) crushing raw materials: filling cleaned and peeled semen Tritici Aestivi germ and semen Sesami with good quality into grinding tank, maintaining at 160 deg.C and 1.2MPa for 15min, and instantly blasting to obtain ground material; wherein the weight ratio of the wheat germ to the sesame is 9: 1.5;
(2) microwave treatment: irradiating the raw material powder obtained in the step (1) for 80 seconds in a microwave environment of 1000 MHz;
(3) compound enzymolysis oil extraction: taking the raw material powder subjected to microwave treatment in the step (2), and mixing the raw material powder in mass ratio: adding water according to the proportion of 1:8, and then adding a complex enzyme of cellulase, neutral protease and ALCALASE2.4L protease for hydrolysis, wherein the enzymolysis temperature is 45 ℃ and the enzymolysis time is 5 h; centrifuging at 8000r/min for 25min to obtain free embryo oil, emulsion, enzymolysis solution and residue; the weight ratio of the cellulase to the protease is 1: 4;
(4) performing microwave treatment on the emulsion, wherein the microwave power is 480W, the microwave time is 20min, then adding ethanol, the concentration of the added ethanol is 70%, and then performing centrifugal separation to obtain free germ oil, wherein the adding amount of the ethanol is 0.3L/Kg of the volume of the ethanol/the weight of the emulsion;
(5) combining the free germ oil obtained in the steps (3) and (4), heating to 75 ℃, adding saline water with the mass concentration of 8% and the temperature of 75 ℃, stirring for 15min under the condition of 55r/min, then adding hot water with the temperature of 75 ℃ into the oil material, stirring for 10min under the condition of 30r/min, standing for 2h, discharging water and precipitate, and obtaining the oil material after water washing; the addition amount of the saline water is 3.5 percent of the free germ oil;
(6) and (3) decoloring treatment: pumping the water-washed oil obtained in the step (5) into a decoloring tank under a vacuum condition, heating the oil to 85 ℃ while stirring, closing a heat conduction valve, and preserving the heat for 20min by using waste heat; then absorbing the activated carbon into a decoloring tank, maintaining for 25min, and filtering after decoloring;
(7) deodorizing the oil material decolorized in the step (6) at the temperature of 170 ℃ and the vacuum degree of 0.10 MPa;
(8) and cooling the deodorized oil to room temperature by circulating cooling water, and sieving with a 400-mesh sieve to obtain the wheat germ compound oil.
Example 4
A method for extracting wheat germ compound oil comprises the following steps:
(1) crushing raw materials: filling cleaned and peeled semen Tritici Aestivi germ and semen Sesami with good quality into grinding tank, maintaining at 160 deg.C and 1.2MPa for 15min, and instantly blasting to obtain ground material; wherein the weight ratio of the wheat germ to the sesame is 10: 1;
(2) microwave treatment: irradiating the raw material powder obtained in the step (1) for 80 seconds in a microwave environment of 1000 MHz;
(3) compound enzymolysis oil extraction: taking the raw material powder subjected to microwave treatment in the step (2), and mixing the raw material powder in mass ratio: adding water according to the proportion of 1:8, and then adding a complex enzyme of cellulase, neutral protease, papain and ALCALASE2.4L protease for hydrolysis, wherein the enzymolysis temperature is 45 ℃ and the enzymolysis time is 5 h; centrifuging at 8000r/min for 25min to obtain free embryo oil, emulsion, enzymolysis solution and residue; the weight ratio of the cellulase to the protease is 1: 5;
(4) performing microwave treatment on the emulsion, wherein the microwave power is 450W, the microwave time is 20min, then adding ethanol, the concentration of the added ethanol is 70%, and then performing centrifugal separation to obtain free germ oil, wherein the adding amount of the ethanol is 0.4L/Kg of the volume of the ethanol/the weight of the emulsion;
(5) combining the free germ oil obtained in the steps (3) and (4), heating to 75 ℃, adding saline water with the mass concentration of 7% and the temperature of 75 ℃, stirring for 15min under the condition of 55r/min, then adding hot water with the temperature of 75 ℃ into the oil material, stirring for 10min under the condition of 30r/min, standing for 2h, discharging water and precipitate, and obtaining the oil material after water washing; the addition amount of the saline water is 3% of the free germ oil;
(6) and (3) decoloring treatment: pumping the water-washed oil obtained in the step (5) into a decoloring tank under a vacuum condition, heating the oil to 85 ℃ while stirring, closing a heat conduction valve, and preserving the heat for 20min by using waste heat; then absorbing the activated carbon into a decoloring tank, maintaining for 25min, and filtering after decoloring;
(7) deodorizing the oil material decolorized in the step (6) at the temperature of 180 ℃ and the vacuum degree of 0.08 MPa;
(8) and cooling the deodorized oil to room temperature by circulating cooling water, and sieving by a 300-mesh sieve to obtain the wheat germ compound oil.
Comparative example 1
A method for extracting wheat germ oil comprises the following steps:
(1) crushing raw materials: filling clean wheat germs with good quality into a grinding tank, maintaining at 150 deg.C and 1.3MPa for 15min, and instantaneously blasting to obtain ground raw material;
(2) microwave treatment: irradiating the raw material powder obtained in the step (1) for 80 seconds in a microwave environment of 1000 MHz;
(3) compound enzymolysis oil extraction: taking the raw material powder subjected to microwave treatment in the step (2), and mixing the raw material powder in mass ratio: adding water according to the proportion of 1:10, and then adding a complex enzyme of cellulase and neutral protease for hydrolysis, wherein the enzymolysis temperature is 45 ℃, and the enzymolysis time is 5 hours; centrifuging at 8000r/min for 25min to obtain free embryo oil, emulsion, enzymolysis solution and residue; the weight ratio of the cellulase to the protease is 1: 5;
(4) performing microwave treatment on the emulsion, wherein the microwave power is 450W, the microwave time is 20min, then adding ethanol, the concentration of the added ethanol is 70%, and then performing centrifugal separation to obtain free germ oil, wherein the adding amount of the ethanol is 0.3L/Kg of the volume of the ethanol/the weight of the emulsion;
(5) combining the free germ oil obtained in the steps (3) and (4), heating to 75 ℃, adding saline water with the mass concentration of 6% and the temperature of 75 ℃, stirring for 15min under the condition of 55r/min, then adding hot water with the temperature of 75 ℃ into the oil material, stirring for 10min under the condition of 30r/min, standing for 2h, discharging water and precipitate, and obtaining the oil material after water washing; the addition amount of the saline water is 3% of the free germ oil;
(6) and (3) decoloring treatment: pumping the water-washed oil obtained in the step (5) into a decoloring tank under a vacuum condition, heating the oil to 85 ℃ while stirring, closing a heat conduction valve, and preserving the heat for 20min by using waste heat; then absorbing the activated carbon into a decoloring tank, maintaining for 25min, and filtering after decoloring;
(7) deodorizing the oil material decolorized in the step (6) at the temperature of 180 ℃ and the vacuum degree of 0.08 MPa;
(8) and cooling the deodorized oil to room temperature by circulating cooling water, and sieving by a 300-mesh sieve to obtain the wheat germ oil.
Comparative example 2
A method for extracting wheat germ compound oil comprises the following steps:
(1) crushing raw materials: filling cleaned and peeled wheat germs and sesame seeds with good quality into a grinding tank, and carrying out superfine grinding to 1000 meshes; wherein the weight ratio of the wheat germ to the sesame is 6: 1;
(2) compound enzymolysis oil extraction: taking the raw material powder subjected to crushing treatment in the step (1), and mixing the raw material powder with the following materials in mass ratio: adding water according to the proportion of 1:10, and then adding a complex enzyme of cellulase, neutral protease and ALCALASE2.4L protease for hydrolysis, wherein the enzymolysis temperature is 45 ℃ and the enzymolysis time is 5 h; centrifuging at 8000r/min for 25min to obtain free embryo oil, emulsion, enzymolysis solution and residue; the weight ratio of the cellulase to the protease is 1: 5;
(3) performing microwave treatment on the emulsion, wherein the microwave power is 450W, the microwave time is 20min, then adding ethanol, the concentration of the added ethanol is 70%, and then performing centrifugal separation to obtain free germ oil, wherein the adding amount of the ethanol is 0.3L/Kg of the volume of the ethanol/the weight of the emulsion;
(4) combining the free germ oil obtained in the step (2) and the step (3), heating to 75 ℃, adding saline water with the mass concentration of 6% and the temperature of 75 ℃, stirring for 15min under the condition of 55r/min, then adding hot water with the temperature of 75 ℃ into the oil material, stirring for 10min under the condition of 30r/min, standing for 2h, discharging water and precipitate, and obtaining the oil material after water washing; the addition amount of the saline water is 3% of the free germ oil;
(5) and (3) decoloring treatment: pumping the water washing oil obtained in the step (4) into a decoloring tank under a vacuum condition, heating the oil to 85 ℃ while stirring, closing a heat conduction valve, and preserving the heat for 20min by using waste heat; then absorbing the activated carbon into a decoloring tank, maintaining for 25min, and filtering after decoloring;
(6) deodorizing the oil material decolorized in the step (5) at the temperature of 180 ℃ and the vacuum degree of 0.08 MPa;
(7) and cooling the deodorized oil to room temperature by circulating cooling water, and sieving by a 300-mesh sieve to obtain the wheat germ compound oil.
The performance tests carried out on the oils extracted in examples 1 to 4 and comparative examples 1 to 2, the results of which are shown in the following table:
finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (6)
1. The extraction method of wheat germ compound oil is characterized by comprising the following steps:
(1) crushing raw materials: placing cleaned and peeled semen Sesami and wheat germ with good quality into a pulverizing tank, maintaining at 160 deg.C and 1.2-1.6MPa for 10-20min, and instantly blasting to obtain pulverized raw material;
(2) microwave treatment: irradiating the raw material powder obtained in the step (1) for 60-120 seconds in a microwave environment of 900-1200 MHz;
(3) compound enzymolysis oil extraction: taking the raw material powder subjected to microwave treatment in the step (2), and mixing the raw material powder in mass ratio: adding water in a ratio of 1-2: 8-20, and then adding a complex enzyme of cellulase and protease for hydrolysis; after enzymolysis, carrying out centrifugal separation for 20-30min under the condition that the centrifugal speed is 5000-10000 r/min to obtain free germ oil, emulsion, enzymatic hydrolysate and residues;
(4) performing microwave treatment on the emulsion, wherein the microwave power is 400-500W, the microwave time is 10-30min, then adding ethanol, the concentration of the added ethanol is 60% -80%, and then performing centrifugal separation to obtain free germ oil;
(5) combining the free germ oil obtained in the steps (3) and (4), heating to 70-80 ℃, adding saline water at the temperature of 70-80 ℃, stirring for 15-20min under the condition of 50-60r/min, then adding hot water at the temperature of 70-80 ℃ into the oil material, stirring for 8-12min under the condition of 25-35r/min, standing for 2-3h, discharging water and precipitate, and obtaining the oil material after water washing;
(6) and (3) decoloring treatment: pumping the water-washed oil obtained in the step (5) into a decoloring tank under a vacuum condition, heating the oil to 80-90 ℃ while stirring, closing a heat conduction valve, and preserving heat for 10-30min by using waste heat; then absorbing the activated carbon into a decoloring tank, maintaining for 20-30min, and filtering after decoloring;
(7) deodorizing the oil material decolorized in the step (6) at the temperature of 160-;
(8) cooling the deodorized oil to room temperature by cooling water circulation, and sieving with a 200-mesh and 400-mesh sieve to obtain the wheat germ compound oil;
wherein the protease in the step (3) comprises acid protease, papain, neutral protease and ALCALASE2.4L protease; the enzymolysis temperature is 40-60 ℃, and the enzymolysis time is 4-8 h.
2. The method for extracting wheat germ composite oil as claimed in claim 1, wherein the weight ratio of wheat germ to sesame in step (1) is 5-10: 1-2.
3. The method for extracting wheat germ composite oil as claimed in claim 1, wherein the weight ratio of cellulase to protease in step (3) is 1: 4-6, and the using amount of the complex enzyme is 2-4% of the weight of the raw material powder.
4. The method for extracting wheat germ composite oil as claimed in claim 3, wherein the amount of ethanol added in step (4) is 0.2-0.4L/Kg ethanol volume/emulsion weight.
5. The method for extracting wheat germ composite oil as claimed in claim 4, wherein the mass concentration of the saline water in step (5) is 5-10%, and the amount of the saline water added is 2-4% of the free germ oil.
6. Wheat germ complex oil extracted by the method for extracting wheat germ complex oil according to any one of claims 1 to 5.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102816633A (en) * | 2012-08-15 | 2012-12-12 | 东北农业大学 | Method for synchronously extracting corn germ oil and separation protein by using alcohol-assisted enzyme method |
| CN103113978A (en) * | 2013-03-14 | 2013-05-22 | 山东省农业科学院农产品研究所 | Method for demulsification for extracting wheatgerm oil by using aqueous enzymatic method |
| CN103881802A (en) * | 2014-04-09 | 2014-06-25 | 杨凌飘香食用油脂有限公司 | Method for extracting black wheat germ oil |
| CN105969511A (en) * | 2016-05-26 | 2016-09-28 | 安徽科技学院 | Sesame oil extraction method |
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| CN102816633A (en) * | 2012-08-15 | 2012-12-12 | 东北农业大学 | Method for synchronously extracting corn germ oil and separation protein by using alcohol-assisted enzyme method |
| CN103113978A (en) * | 2013-03-14 | 2013-05-22 | 山东省农业科学院农产品研究所 | Method for demulsification for extracting wheatgerm oil by using aqueous enzymatic method |
| CN103881802A (en) * | 2014-04-09 | 2014-06-25 | 杨凌飘香食用油脂有限公司 | Method for extracting black wheat germ oil |
| CN105969511A (en) * | 2016-05-26 | 2016-09-28 | 安徽科技学院 | Sesame oil extraction method |
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