CN108899106A - It is a kind of for protecting the devices and methods therefor of cell - Google Patents
It is a kind of for protecting the devices and methods therefor of cell Download PDFInfo
- Publication number
- CN108899106A CN108899106A CN201810750625.8A CN201810750625A CN108899106A CN 108899106 A CN108899106 A CN 108899106A CN 201810750625 A CN201810750625 A CN 201810750625A CN 108899106 A CN108899106 A CN 108899106A
- Authority
- CN
- China
- Prior art keywords
- cell
- protecting
- devices
- methods therefor
- optical tweezer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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Classifications
-
- G—PHYSICS
- G21—NUCLEAR PHYSICS; NUCLEAR ENGINEERING
- G21K—TECHNIQUES FOR HANDLING PARTICLES OR IONISING RADIATION NOT OTHERWISE PROVIDED FOR; IRRADIATION DEVICES; GAMMA RAY OR X-RAY MICROSCOPES
- G21K1/00—Arrangements for handling particles or ionising radiation, e.g. focusing or moderating
- G21K1/006—Manipulation of neutral particles by using radiation pressure, e.g. optical levitation
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
Abstract
The invention discloses a kind of for protecting the devices and methods therefor of cell; including cytoprotection device; the cytoprotection device includes syringe, electron microscope and the optical tweezer transmitter and Cytology Lab being arranged on electron microscope; the Cytology Lab includes cell storage chamber, cell Processing Room and cell storage room, and the syringe includes the injection needle that injection tube and the plunger being arranged on injection tube are connected with injection tube.Then the present invention wraps up layer of protecting liquid on cell, for protecting cell, promotes the vigor of cell, slow down the death of cell by coming out cell capture.
Description
Technical field
The present invention relates to cell technology fields more particularly to a kind of for protecting the devices and methods therefor of cell.
Background technique
With the development of science and technology, the mankind are more and more frequent to the manipulation of microcosmos, therefore, to the need of such tool
Ask also growing day by day, and laser tweezers can be used to manipulate nano particle as a kind of tool being wherein widely adopted
(Nanoparticle), nano wire (Nanowire), nanotube (Nanotube), large biological molecule and cell etc., make the mankind more
Add and understand microcosmic dynamic process and interaction, and carries out arrangement as needed to microcosmic research object.Thin
In born of the same parents and biomolecule research, biologist wants to see the effect of molecule and cell, the effect of cell and cell, molecule
With the effect of molecule, these processes can be observed by optical optical tweezers system, or even measure phase in these interaction processes
The real-time change of interreaction force, to understand entire mechanism.Using microscopically observation biological cell or biology point
The period of the day from 11 p.m. to 1 a.m, due to the variation of temperature, cell or molecule can run out of the microscopical visual field, thus operator frequently to adjust it is aobvious
The visual field of micro mirror, but if it will vise biological cell or molecule, make to operate using optical tweezer come immobilizing biological samples
Person can preferably observe cell and molecule, and will reduce environmental change bring in this way influences, and keep observed result relatively reliable.
Traditional optical optical tweezers system is based on microscope and constructs, and is a kind of structural schematic diagram of optical optical tweezers system, working principle
As follows, the light beam launched from laser 1 is through reflecting mirror M2, lens L1, lens L2, digital synthesizer (Digital
Synthesiser) 2, dichroic beam splitting is incident to after the shaping of components such as reflecting mirror M2, lens L3, lens L4, reflecting mirror M3
On device 3, dichroic beam splitters 3 reflect the light wave for being used to form optical tweezer to the object lens 4 ' of inverted microscope 4, and laser beam is through object
Mirror 4 ' assemble after formed optical trap with capture be placed in 5 Shangdi large biological molecule of sample stage, cell, nanotube etc. it
The sample to be observed of class, researcher can be observed by the eyepiece of inverted microscope 4.Generally, optical optical tweezers system is also matched
It is equipped with imaging optical system, in order to the manipulation and observation of researcher comprising have imaging illumination light source 6 and laser filtering
The light beam of piece 7, imaging illumination light source 6 injects dichroic beam splitters 3 through object lens 4 ', transmits through dichroic beam splitters 3 and through laser
The manipulation situation on sample stage 5 is imaged in into CCD photographic device 8 after the reflection of 7 part of filter plate, and is shown in image with CCD and fill
The display device 9 of 8 connections is set, researcher can observe sample by display device 9.It is similar with above-mentioned optical optical tweezers system
As patented technology, such as State Intellectual Property Office in the Patent No. ZL02808941.3 of Granted publication on May 24th, 2006
Optical optical tweezers system disclosed in patent of invention.
Be relevant to the technology based on microscopical optical optical tweezers system and tended to be mature, but its there are still intrinsic in structure
Drawback mainly has：Optical device used in optical optical tweezers system is more, causes the coupling between each device and connects complex, essence
The difficulty of close adjustment and collimation adjustment is larger, to reduce the reliability of system, system operatio is more troublesome with maintenance；With light
Main body of the microscope as optical optical tweezers system is learned, a light inlet of optical microscopy must be occupied, influence microscope light source
It is equipped with, also, for the research field with a variety of operating function demands, lacks in structure flexible.
In general, cell is the freezing under -196 DEG C of extremely low temperature, and acquisition of thawing rapidly when needing living cells.
Although the survival rate of frozen-thawed cell (frozen-and-then-thawed cells) is skilled according to cell type or operator's
Degree and different, but normal and useful cell, non-cancer cell, survival rate it is very low.In addition, only need transport or
A few houres or several days are stored, and in the case where not long-term transport or storage, cryopreservation (cryopreservation) method
It is unpractiaca.
Based on described above, our the technologies that it is necessary to propose that optical tweezer technology is utilized protect after cell separation.
Summary of the invention
It is an object of the invention to overcome problem above of the existing technology, provide a kind of for protecting the device of cell
And its then method, the present invention wrap up layer of protecting liquid on cell, for protecting cell, mention by coming out cell capture
The vigor for rising cell, slows down the death of cell.
To realize above-mentioned technical purpose and the technique effect, the invention is realized by the following technical scheme：
It is a kind of for protecting the devices and methods therefor of cell, including cytoprotection device, the cytoprotection device includes injection
Device, electron microscope and the optical tweezer transmitter and Cytology Lab being arranged on electron microscope, the Cytology Lab include that cell is deposited
Put room, cell Processing Room and cell storage room, the syringe include injection tube and the plunger being arranged on injection tube and
The injection needle of injection tube connection, the method for cytoprotection include the following steps：
1) need to cell to be protected to be placed into cell storage chamber stand-by；
2) using cell to be protected is needed in optical tweezer clamping cell storage chamber, cell is moved in cell Processing Room；
3) the cell injection protection liquid using syringe into cell Processing Room, makes the periphery for protecting liquid to be wrapped in cell；
4) there is the cell of protection liquid using optical tweezer clamping, be moved into cell storage room, stored and protected.
Preferably, the optical tweezer transmitter is using tunable laser source or the optical tweezer transmitter of adjustable intensity laser light source.
Preferably, the bore of the injection needle is arranged in 10um to 50um.
Preferably, the protection liquid is set as cell protecting factor, cell protecting factor in aweto by extracting.
Preferably, the electron microscope includes pedestal, places the moving stage of Cytology Lab and be located at mobile carry
The lens barrel for being equipped with optical tweezer transmitter on object platform is provided with imaging sensor in the lens barrel, and described image sensor passes through
Computer is connect with display.
Preferably, being additionally provided with thermostatic chamber on the moving stage, the thermostatic chamber is connect with Cytology Lab.
Preferably, being provided with CCD camera or display monitor central monitoring system or video recorder in the lens barrel.
Preferably, the side of the lens barrel is provided with eyepiece, the other side is provided with object lens, and the object lens are located at Cytology Lab
Top.
The beneficial effects of the invention are as follows：
1. then the present invention wraps up layer of protecting liquid on cell, for protecting cell, is promoted thin by coming out cell capture
The vigor of born of the same parents slows down the death of cell；
2. capturing, precision is high, adjustability is strong, not easy damaged cells, realizes the accurate capture to cell, carries, screening operation；
3. cytoprotection device is configured convenient for researcher and is used；
4. realizing the combination of optical tweezer technology and injector technology and integrating, expands and enhance optical tweezer technology and injector technology
Function, two kinds of high-precision biological micromanipulator device equipment are integrated in one, the function of system is extended, improves system
Cost performance greatly improves the protective effect of capture rate and cell.
The above description is only an overview of the technical scheme of the present invention, in order to better understand the technical means of the present invention,
And can be implemented in accordance with the contents of the specification, with presently preferred embodiments of the present invention and attached drawing is cooperated to be described in detail below.This hair
Bright specific embodiment is shown in detail by following embodiment and its attached drawing.
Detailed description of the invention
The drawings described herein are used to provide a further understanding of the present invention, constitutes part of this application, this hair
Bright illustrative embodiments and their description are used to explain the present invention, and are not constituted improper limitations of the present invention.In the accompanying drawings：
Fig. 1 is schematic diagram of the present invention；
Fig. 2 is structural schematic diagram of the present invention；
Fig. 3 is Cytology Lab schematic diagram of the present invention.
Figure label explanation：Syringe 1, electron microscope 2, optical tweezer transmitter 3, Cytology Lab 4, cell storage chamber 5, cell
Processing Room 6, cell storage room 7, injection tube 101, plunger 102, injection needle 103, cell 8 protect liquid 9, pedestal 10, mobile loading
Platform 11, lens barrel 12, imaging sensor 13, computer 14, display 15, thermostatic chamber 16, eyepiece 17, object lens 18, deflecting plate 19.
Specific embodiment
The invention will be further described with reference to the accompanying drawing：
It is a kind of for protecting the devices and methods therefor of cell, including cytoprotection device, the cell referring to figs. 1 to shown in Fig. 3
Protective device includes syringe 1, electron microscope 2 and the optical tweezer transmitter 3 and Cytology Lab 4 being arranged on electron microscope 2,
The Cytology Lab 4 includes cell storage chamber 5, cell Processing Room 6 and cell storage room 7, and the syringe 1 includes injection tube
101 and be arranged on injection tube 101 plunger 102 and injection tube 101 connection injection needle 103, the method for cytoprotection
Include the following steps：
1) need to cell 8 to be protected to be placed into cell storage chamber 5 stand-by；
2) using cell 8 to be protected is needed in optical tweezer clamping cell storage chamber 5, cell 8 is moved in cell Processing Room 6；
3) the injection protection liquid 9 of cell 8 using syringe 1 into cell Processing Room 6, makes the periphery for protecting liquid 9 to be wrapped in cell 8；
4) there is the cell 8 of protection liquid 9 using optical tweezer clamping, be moved into cell storage room 7, stored and protected.
Preferably, the optical tweezer transmitter 3 is using the optical tweezer of tunable laser source or adjustable intensity laser light source hair
Emitter 3.
Preferably, the bore of the injection needle 103 is arranged in 10um to 50um.
Preferably, the protection liquid 9 is set as cell protecting factor, cell protecting factor in aweto by mentioning
It takes.
Preferably, the electron microscope 2 includes pedestal 10, places the moving stage 11 of Cytology Lab 4 and be located at
The lens barrel 12 for being equipped with optical tweezer transmitter 3 on moving stage 11 is provided with imaging sensor 13 in the lens barrel 12, described
Imaging sensor 13 is connect by computer 14 with display 15.
Preferably, being additionally provided with thermostatic chamber 16 on the moving stage 11, the thermostatic chamber 16 connects with Cytology Lab 4
It connects.
Preferably, being provided with CCD camera or display monitor central monitoring system or video recorder in the lens barrel 12.
Preferably, the side of the lens barrel 12 is provided with eyepiece 17, the other side is provided with object lens 18,18, the object lens
In the top of Cytology Lab 4.
Specific embodiment：
In actual use, cytoprotection device includes syringe, electron microscope and the light being arranged on electron microscope
Tweezer transmitter and Cytology Lab, need to cell to be protected to be placed into cell storage chamber stand-by；Cell storage chamber is clamped using optical tweezer
Middle need cell to be protected, cell is moved in cell Processing Room；It is sprayed using cell of the syringe into cell Processing Room
Liquid is protected, the periphery for protecting liquid to be wrapped in cell is made；There is the cell of protection liquid using optical tweezer clamping, be moved into cell storage
It deposits in room, is stored and protected.
The above description is merely a specific embodiment, but scope of protection of the present invention is not limited thereto, any
Those familiar with the art in the technical scope disclosed by the present invention, can easily think of the change or the replacement, and should all contain
Lid is within protection scope of the present invention.Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (8)
1. a kind of for protecting the devices and methods therefor of cell, it is characterised in that：Including cytoprotection device, the cytoprotection
Device includes syringe（1）, electron microscope（2）And it is arranged in electron microscope（2）On optical tweezer transmitter（3）And cell
Room（4）, the Cytology Lab（4）Including cell storage chamber（5）, cell Processing Room（6）And cell storage room（7）, the injection
Device（1）Including injection tube（101）And it is arranged in injection tube（101）On plunger（102）And injection tube（101）The injection of connection
Needle（103）, the method for cytoprotection includes the following steps：
It will need cell to be protected（8）It is placed into cell storage chamber（5）For use；
Cell storage chamber is clamped using optical tweezer（5）Middle need cell to be protected（8）, by cell（8）It is moved to cell Processing Room（6）
In；
Use syringe（1）To cell Processing Room（6）In cell（8）Injection protection liquid（9）, make to protect liquid（9）It is wrapped in thin
Born of the same parents（8）Periphery；
There is protection liquid using optical tweezer clamping（9）Cell（8）, it is moved into cell storage room（7）In, it is stored and is protected
Shield.
2. according to claim 1 a kind of for protecting the devices and methods therefor of cell, it is characterised in that：The optical tweezer hair
Emitter（3）Using tunable laser source or the optical tweezer transmitter of adjustable intensity laser light source（3）.
3. according to claim 1 a kind of for protecting the devices and methods therefor of cell, it is characterised in that：The injection needle
（103）Bore be arranged in 10um to 50um.
4. according to claim 1 a kind of for protecting the devices and methods therefor of cell, it is characterised in that：The step 3
In protection liquid（9）It is set as cell protecting factor, cell protecting factor in aweto by extracting.
5. according to claim 1 a kind of for protecting the devices and methods therefor of cell, it is characterised in that：The electronic display
Micro mirror（2）Including pedestal（10）, place Cytology Lab（4）Moving stage（11）And it is located at moving stage（11）On peace
Equipped with optical tweezer transmitter（3）Lens barrel（12）, the lens barrel（12）Inside it is provided with imaging sensor（13）, described image sensor
（13）Pass through computer（14）With display（15）Connection.
6. according to claim 5 a kind of for protecting the devices and methods therefor of cell, it is characterised in that：The mobile load
Object platform（11）On be additionally provided with thermostatic chamber（16）, the thermostatic chamber（16）With Cytology Lab（4）Connection.
7. according to claim 5 a kind of for protecting the devices and methods therefor of cell, it is characterised in that：The lens barrel
（12）In be provided with CCD camera or display monitor central monitoring system or video recorder.
8. according to claim 5 a kind of for protecting the devices and methods therefor of cell, it is characterised in that：The lens barrel
（12）Side be provided with eyepiece（17）, the other side is provided with object lens（18）, the object lens（18）Positioned at Cytology Lab（4）It is upper
Side.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810750625.8A CN108899106A (en) | 2018-07-10 | 2018-07-10 | It is a kind of for protecting the devices and methods therefor of cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810750625.8A CN108899106A (en) | 2018-07-10 | 2018-07-10 | It is a kind of for protecting the devices and methods therefor of cell |
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|---|---|
| CN108899106A true CN108899106A (en) | 2018-11-27 |
Family
ID=64348929
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| Application Number | Title | Priority Date | Filing Date |
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| CN201810750625.8A Pending CN108899106A (en) | 2018-07-10 | 2018-07-10 | It is a kind of for protecting the devices and methods therefor of cell |
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