CN108887446A - A kind of method that drink drinks preceding fast eliminating caffeine - Google Patents

A kind of method that drink drinks preceding fast eliminating caffeine Download PDF

Info

Publication number
CN108887446A
CN108887446A CN201810787875.9A CN201810787875A CN108887446A CN 108887446 A CN108887446 A CN 108887446A CN 201810787875 A CN201810787875 A CN 201810787875A CN 108887446 A CN108887446 A CN 108887446A
Authority
CN
China
Prior art keywords
caffeine
drink
drunk
molecularly imprinted
fast eliminating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201810787875.9A
Other languages
Chinese (zh)
Inventor
邓慧芸
谢练武
吴茂
范亚军
郭亚平
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Central South University of Forestry and Technology
Original Assignee
Central South University of Forestry and Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Central South University of Forestry and Technology filed Critical Central South University of Forestry and Technology
Priority to CN201810787875.9A priority Critical patent/CN108887446A/en
Publication of CN108887446A publication Critical patent/CN108887446A/en
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00—Tea; Tea substitutes; Preparations thereof
    • A23F3/36—Reducing or removing alkaloid content; Preparations produced thereby; Extracts or infusions thereof
    • A23F3/38—Reducing or removing alkaloid content from tea extract
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00—Tea; Tea substitutes; Preparations thereof
    • A23F3/36—Reducing or removing alkaloid content; Preparations produced thereby; Extracts or infusions thereof
    • A23F3/38—Reducing or removing alkaloid content from tea extract
    • A23F3/385—Reducing or removing alkaloid content from tea extract using flocculating, precipitating, adsorbing or complex-forming agents, or ion-exchangers
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F2/00—Processes of polymerisation
    • C08F2/44—Polymerisation in the presence of compounding ingredients, e.g. plasticisers, dyestuffs, fillers
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F220/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical or a salt, anhydride ester, amide, imide or nitrile thereof
    • C08F220/02—Monocarboxylic acids having less than ten carbon atoms; Derivatives thereof
    • C08F220/04—Acids; Metal salts or ammonium salts thereof
    • C08F220/06—Acrylic acid; Methacrylic acid; Metal salts or ammonium salts thereof
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
    • C08K7/00—Use of ingredients characterised by shape
    • C08K7/22—Expanded, porous or hollow particles
    • C08K7/24—Expanded, porous or hollow particles inorganic
    • C08K7/26—Silicon- containing compounds

Landscapes

  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

A kind of method of fast eliminating caffeine before being drunk the invention discloses drink.Utilize surface molecule print technology, using caffeine as template molecule, α-methacrylic acid be crosslinking agent as function monomer, ethylene glycol dimethacrylate, azo isobutyronitrile is initiator, prepare mesoporous silicon substrate surface molecular engram microsphere, the molecular engram microsphere of preparation is as solid phase extraction material, it is sealed against in composite fibre filter membrane pouch, then the caffeine before drink is drunk in fast eliminating drink.This method is not only convenient and efficient, but also on other active components without influence, and therefore, the present invention had both removed the cumbersome technique of caffeine removing in production process from, also reduces production cost, and can guarantee original quality of drink.

Description

A kind of method that drink drinks preceding fast eliminating caffeine
Technical field
The side of fast eliminating caffeine before being drunk the present invention relates to a kind for the treatment of process of drink more particularly to a kind of drink Method.
Background technique
Entitled 1,3, the 7- trimethyl xanthine of caffeine (caffeine) chemistry, is a kind of astragalus alkaloid compound, Appropriate intake can dispel fatigue, make nervous excitation, but large dosage intake or long-time service can damage the vitals such as liver, stomach, kidney, Induce a series of serious diseases.However, all containing a large amount of caffeines in tealeaves, coffee and some cola beverages.Such as：Tea Although leaf contains the various bioactive components such as tea polyphenols, tea polysaccharide, vitamin, amino acid, wherein the alkaloids such as caffeine Ingredient accounts for the 3%~5% of dry weight of tea leaves.Therefore, the content for reducing caffeine in tealeaves and tea beverage is of great significance.
The caffeine in tealeaves is removed, the tea for producing low-caffeine has become a development new direction of Tea Production.According to Report, the main method that caffeine is removed from tealeaves have flooding removing, precipitation method removing, supercritical CO2Extraction and micro- life Object degrading caffeine etc..
Wherein, water logging extraction method is easy to operate, practical but be suitable only for fresh leaf and water-removing leaves, is not suitable for rubbing leaf And dried leaf.The precipitation method can also be used for the removing of caffeine in tea, but in tea beverage active constituent loss it is very big.It is super to face Boundary's extraction is larger to the effective component loss in tealeaves.Microbial degradation method is still immature at present.The above method is basic Content of caffeine is all removed or reduced during Tea Production, has certain removal effect, but can make complex production process, even Tea leaf quality is caused to decline, production cost is excessively high.
Summary of the invention
In order to solve the above technical problems, the present invention provides a kind of method that drink drinks preceding fast eliminating caffeine, both may be used Caffeine is quickly and easily removed, and does not influence original quality of tealeaves, is a kind of very effective method.
The technical solution adopted by the present invention：
A kind of method that drink drinks preceding fast eliminating caffeine, specifically includes following steps：
A) production of caffeine packaging bag is removed：Selection composite fibre filter membrane is material, extends side doubling, with continuous sealing Machine presses its two sides, and packaging bag is made；
B) pack of molecularly imprinted microspheres on surface：100~200mg of molecularly imprinted microspheres on surface is taken, above-mentioned removing coffee is packed into Coffee protrudes into open end because of packaging bag, cotton thread, presses them with continuous sealer and seals, packaging bag is placed in polybag very Empty package；
C) in drink caffeine removing：Before drink is drunk, the removing coffee of molecularly imprinted microspheres on surface will be first housed Because packet is added in drink, cotton thread extended end, to take, after 3~5min, takes out close to rim of a cup, and the de- of caffeine can be completed It removes.
As a further improvement of the present invention, in above-mentioned steps b), the preparation of molecularly imprinted microspheres on surface：It is printed by surface Mark polymerization, with the modified mesoporous SiO of double bond2Microballoon is carrier, and for coffee because of template molecule, α-methacrylic acid is function list Body, ethylene glycol dimethacrylate are crosslinking agent, and azo isobutyronitrile is initiator, and acetonitrile is pore-foaming agent, and preparation is containing template point The molecularly imprinted microspheres on surface of son, be then washed till supernatant with acetonitrile transparent, again with methanol and acetic acid solution, completely removing template Molecule is dried in vacuum overnight finally, being washed till neutrality with methanol, obtains molecularly imprinted microspheres on surface mSiO2@MIPs.Pass through polymerization Reaction generates high molecular polymer, non-volatility, and high molecular polymer is safe and effective.
In above-mentioned reaction, the molar ratio 2-3 of caffeine and function monomer α-methacrylic acid：8-12；What double bond was modified Mesoporous SiO2Microballoon, crosslinking agent ethylene glycol dimethacrylate, initiator azo isobutyronitrile mass ratio 10-15：20-30：4- 6。
As a further improvement of the present invention, above-mentioned mesoporous SiO2The double bond modification procedure of microballoon：By mesoporous SiO2It is suspended from 3- In (trimethoxy silicon substrate)-propyl methacrylate solution, stirring, centrifuge separation is collected, and is washed repeatedly, vacuum drying is ?.
Mesoporous SiO2The double bond modification of microballoon is specific as follows：Using sol preparation, by mesoporous SiO2It is suspended from 3- (trimethoxy Base silicon substrate) in-propyl methacrylate solution, 5~8h is stirred at 40~50 DEG C, separates and collects, washes repeatedly, 40~60 DEG C It is dried in vacuo to obtain the final product.
As a further improvement of the present invention, the diameter of molecularly imprinted microspheres on surface is at 0.3~0.4 μm, to prevent microballoon It leaks out, aperture need to be selected to be less than the filter membrane of microspherulite diameter, composite fibre filter sizes should be less than 0.15 μm.
As a further improvement of the present invention, in order to ensure molecular engram microsphere does not leak out, the packaging bag of caffeine is removed It is that composite fibre filter membrane material with two layers of aperture less than 0.15 μm is made up of continuous sealer pressing.
100~200mg of molecularly imprinted microspheres on surface is taken, above-mentioned removing caffeine packaging bag is packed into.It is packed into packaging bag surface The quality of molecular engram microsphere be by statistical method, it is micro- according to the quality of tealeaves when making tea and content of caffeine and trace What the saturated extent of adsorption of ball calculated.
Compared with prior art, beneficial effects of the present invention：(1) this method is using molecularly imprinted microspheres on surface as Solid Phase Extraction Material eliminates the cumbersome technique that caffeine is removed during Tea Production by caffeine fast eliminating before drink is drunk； (2) this method can not only fast eliminating caffeine, and the other active components in tealeaves are not influenced；(3) de- using this Caffeine is removed except caffeine packet, it is simple and quick, only need a few minutes that can complete, far fewer than boiling water brewing tea after etc. The time drunk is waited, is conducive to popularize.
Detailed description of the invention
Fig. 1 is molecularly imprinted microspheres on surface transmission electron microscope picture of the present invention.
Fig. 2 is the finished figure of present invention removing caffeine packaging bag.
Specific embodiment
In order to deepen the understanding of the present invention, with reference to the accompanying drawing and specific embodiment is described in detail, this explanation The examples are only for explaining the invention described in book, is not used to limit the present invention.
Embodiment 1
The method of fast eliminating caffeine, specifically includes following steps before a kind of drink of the invention is drunk：
(1) preparation of molecularly imprinted microspheres on surface：
A) mesoporous SiO2The double bond of microballoon is modified：By mesoporous SiO2Microballoon (250mg) is suspended from 3- (trimethoxy silicon substrate)-the third Methyl acrylate solution (MPS of 120 μ L is dissolved in 32mL8% acetic acid solution), 40 DEG C of stirring 4h, separates and collects, repeatedly Washing, 40 DEG C of vacuum drying 8h to obtain the final product.
B) preparation of molecularly imprinted microspheres on surface：By caffeine (0.2mmol) and function monomer α-methacrylic acid (0.8mmol) is dissolved in anhydrous acetonitrile (10mL) and carries out pre-assembled, N2After purging, it is put into 3 DEG C of refrigerators and stores 10h, it is spare.So Afterwards, the mesoporous SiO that double bond is modified2Microballoon (40mg), crosslinking agent ethylene glycol dimethacrylate (4mmol) and initiator are even Nitrogen isobutyronitrile (16mg) is dissolved in pore-foaming agent acetonitrile (12mL), is operated on ice, N2Purging is lower to be added above-mentioned pre-assembled solution, then 60 DEG C of constant temperature are stirred to react for 24 hours.After polymerization, separate and collect, it is transparent to be washed till supernatant with acetonitrile, then with methanol-acetic acid (9/1, V/v), template molecule is removed completely.Finally, being washed till neutrality with methanol, 40 DEG C are dried in vacuum overnight, and obtain mSiO2@MIPs。
(2) production of caffeine packet is removed：
A) production of caffeine packaging bag is removed：Choose aperture less than 0.15 μm composite fibre filter membrane (100mm × 50mm) it is material, extends side doubling, its two sides is pressed by continuous sealer, the packaging bag of 50mm × 50mm is made.
B) pack of molecularly imprinted microspheres on surface：The molecularly imprinted microspheres on surface 100mg for taking (1) to prepare is packed into above-mentioned de- Except caffeine packaging bag, the cotton thread of a root long 10cm is protruded into open end, they are pressed with continuous sealer and is sealed, will be packed Bag is placed in vacuum packaging in polybag (see attached drawing 2).
Embodiment 2
The method of fast eliminating caffeine, specifically includes following steps before a kind of drink of the invention is drunk：
(1) preparation of molecularly imprinted microspheres on surface：
A) mesoporous SiO2The double bond of microballoon is modified：By mesoporous SiO2Microballoon (250mg) is suspended from 3- (trimethoxy silicon substrate)-the third Methyl acrylate solution (MPS of 150 μ L is dissolved in 40mL10% acetic acid solution), 50 DEG C of stirring 5h, separates and collects, repeatedly Washing, 50 DEG C of vacuum drying 8h to obtain the final product.
B) preparation of molecularly imprinted microspheres on surface：By caffeine (0.25mmol) and function monomer α-methacrylic acid (1mmol) is dissolved in anhydrous acetonitrile (12mL) and carries out pre-assembled, N2After purging, it is put into 4 DEG C of refrigerators and stores 12h, it is spare.So Afterwards, the mesoporous SiO that double bond is modified2Microballoon (50mg), crosslinking agent ethylene glycol dimethacrylate (5mmol) and initiator are even Nitrogen isobutyronitrile (20mg) is dissolved in pore-foaming agent acetonitrile (15mL), is operated on ice, N2Purging is lower to be added above-mentioned pre-assembled solution, then 60 DEG C of constant temperature are stirred to react for 24 hours.After polymerization, separate and collect, it is transparent to be washed till supernatant with acetonitrile, then with methanol-acetic acid (9/1, V/v), template molecule is removed completely.Finally, being washed till neutrality with methanol, 50 DEG C are dried in vacuum overnight, and obtain mSiO2@MIPs。
(2) production of caffeine packet is removed：
A) production of caffeine packaging bag is removed：Choose aperture less than 0.15 μm composite fibre filter membrane (100mm × 50mm) it is material, extends side doubling, its two sides is pressed by continuous sealer, the packaging bag of 50mm × 50mm is made.
B) pack of molecularly imprinted microspheres on surface：The molecularly imprinted microspheres on surface 150mg for taking (1) to prepare is packed into above-mentioned de- Except caffeine packaging bag, the cotton thread of a root long 10cm is protruded into open end, they are pressed with continuous sealer and is sealed, will be packed Bag is placed in vacuum packaging in polybag (see attached drawing 2).
Embodiment 3
The method of fast eliminating caffeine, specifically includes following steps before a kind of drink of the invention is drunk：
(1) preparation of molecularly imprinted microspheres on surface：
A) mesoporous SiO2The double bond of microballoon is modified：By mesoporous SiO2Microballoon (350mg) is suspended from 3- (trimethoxy silicon substrate)-the third Methyl acrylate solution (MPS of 180 μ L is dissolved in 48mL12% acetic acid solution), 60 DEG C of stirring 6h, separates and collects, repeatedly Washing, 50 DEG C of vacuum drying 8h to obtain the final product.
B) preparation of molecularly imprinted microspheres on surface：By caffeine (0.3mmol) and function monomer α-methacrylic acid (1.2mmol) is dissolved in anhydrous acetonitrile (14mL) and carries out pre-assembled, N2After purging, it is put into 5 DEG C of refrigerators and stores 14h, it is spare.So Afterwards, the mesoporous SiO that double bond is modified2Microballoon (60mg), crosslinking agent ethylene glycol dimethacrylate (6mmol) and initiator are even Nitrogen isobutyronitrile (24mg) is dissolved in pore-foaming agent acetonitrile (18mL), is operated on ice, N2Purging is lower to be added above-mentioned pre-assembled solution, then 60 DEG C of constant temperature are stirred to react for 24 hours.After polymerization, separate and collect, it is transparent to be washed till supernatant with acetonitrile, then with methanol-acetic acid (9/1, V/v), template molecule is removed completely.Finally, being washed till neutrality with methanol, 60 DEG C are dried in vacuum overnight, and obtain mSiO2@MIPs。
(2) production of caffeine packet is removed：
A) production of caffeine packaging bag is removed：Choose aperture less than 0.15 μm composite fibre filter membrane (100mm × 50mm) it is material, extends side doubling, its two sides is pressed by continuous sealer, the packaging bag of 50mm × 50mm is made.
B) pack of molecularly imprinted microspheres on surface：The molecularly imprinted microspheres on surface 200mg for taking (1) to prepare is packed into above-mentioned de- Except caffeine packaging bag, the cotton thread of a root long 10cm is protruded into open end, they are pressed with continuous sealer and is sealed, will be packed Bag is placed in vacuum packaging in polybag (see attached drawing 2).
The above-mentioned composite fibre filter membrane used is nontoxic, 120 DEG C of high temperature resistant, inflammable, not acid and alkali-resistance, not solvent resistant, only Suitable for aqueous solution (pH=2~10), vegetable oil, fruit juice, drinks etc..
Following embodiment is to remove the safety evaluatio experiment of caffeine packet
In order to detect the safety of removing caffeine packet, i.e., when removing caffeine in drink, composite fibre filter membrane packaging Bag dissolves out phenomenon with the presence or absence of poisonous and harmful substance with molecular engram microsphere, is tested as follows：
(1) test grouping
A) healthy adult mouse 20 are selected, weight is 20 ± 2g, and half male and half female divides cage by gender, and weighing number first exists Laboratory standard is fed 5 days, carries out following experiment after being confirmed as healthy mice.
B) the completely random method of dividision into groups is pressed, mouse is divided into two groups, half male and half female, every group 10.
(2) test method
A) mouse is administered according to maximum tolerated dose method in GB 15193.3-2003 using administration by gavage, control group stomach fills Original millet paste, experimental group stomach are filled through removing caffeine packet treated millet paste, and administration 2 times, each administered volume are sooner or later daily 0.4mL/20g weight (human body for being equivalent to 50kg weight daily intakes 2000mL millet paste), successive administration 14 days, does not limit drink Water.
B) macroscopic features, food ration, behavioral activity, the fecal character etc. of each mouse of every day entry, experiment front and back claim its body Weight, the results are shown in Table 1.
C) after 14 days, mouse urine and blood (blood collection method is cut using tail) are acquired, and detect indices, as a result such as table 2 With shown in table 3.
D) after 14 days, all animals is dissected and observe its internal organ situation, dissect preceding fasting 16h or so, not water restriction, as a result As shown in table 4.
The sign situation of change of 1 mouse of table
Table 2 tests the influence to mouse retention conventional index
Table 3 tests the influence to mouse routine blood indexes
Routine blood indexes Control group Test group
Hemoglobin concentration (g/L) 105.24±6.34 104.96±7.04
Red blood cell (× 1012/L) 6.56±0.09 6.72±0.12
Leucocyte (× 109/L) 1.85±0.32 1.91±0.29
Table 4 tests the influence to mice viscera
By observing mouse sign (table 1), control group and experimental mice are in macroscopic features, food ration, fecal character etc. Indifference.For test group mouse, control group mice weight gain is less, but more active, and excitement levels is also higher, can Can explanation decrease through content of caffeine in removing caffeine packet treated millet paste.
The routine urinalysis (table 2) and blood routine (table 3) indices of control group and test group small white mouse are in normal range (NR), And two groups of data are very close, therefore original millet paste and processed millet paste are to the equal nothing of each index of urine and blood of mouse It influences.
It is found through Dissection test (table 4), the liver of control group mice, kidney stomach function regulating are influenced a little, test group mouse Liver, kidney stomach function regulating are then shown normally, thus prove that a large amount of intake internal influential points of viscera of caffeine cause certain damage.The above experiment is also demonstrate,proved The safety of removing caffeine packet is illustrated, noxious material will not be generated during making tea.
Following embodiment is to drink removing caffeine effect assessment experiment (by taking certain brand dark green tea as an example)
20g dark green tea is taken, is added in 500mL boiled water and brews, after 20min, millet paste is divided into two parts.First part (before processing) The direct HPLC of 250mL millet paste analyzes Contents of Main Components；The investment removing caffeine into second portion of (after processing) 250mL millet paste Packet, cotton thread extended end takes out removing caffeine packet after impregnating 5min close to rim of a cup, then row HPLC analyzes Contents of Main Components (table 5).
Table 5 removes the effect of caffeine packet processing dark green tea millet paste
As can be seen from Table 5, after removing caffeine packet processing, the content of caffeine in dark green tea millet paste is decreased obviously, and is taken off Except rate is up to 78.19%.And other active components do not change substantially.Therefore, it is prepared using molecularly imprinted microspheres on surface as raw material Caffeine packet is removed, can realize the fast eliminating of caffeine in the case where not influencing active constituent before drink is drunk, thus Avoid injury caused by long-term a large amount of intake Caffeine On Human bodies.
Why the present invention uses polymerized monomer, crosslinking agent, initiator etc., generates high molecular polymerization by polymerization reaction Object safely and effectively after polymerized monomer aggregates into high molecular polymer, has been subjected to the extracting of sufficient organic solvent, water, small molecule It being completely removed, monomer may have certain volatility, so stimulation respiratory tract, but polymer molecular weight is very big, non-volatility.
Method of the invention is got by following principle：
Molecularly imprinted polymer (MIPs) refers to target molecule and function monomer to be separated by covalently or non-covalently Effect carries out polymer that is pre-assembled, then being copolymerized with crosslinking agent.It is formed after removing target molecule, in polymer and target point Sub- spatial configuration is complementary and " hole " with predetermined action site, therefore imitates to the space structure of target molecule with " memory " It answers, can identify the microsphere in complex sample with high selectivity.Before the polymer is widely used in complex sample analysis Processing, has become the hot spot studied both at home and abroad in recent years, and molecular imprinting technology is combined with solid phase micro-extraction technique, is even more divided New breakthrough from enrichment field.Therefore, molecular imprinting technology is applied to the removing of caffeine, will be a kind of highly effective, energy The method enough promoted, this method, because the molecularly imprinted polymer of template is solid extracting agent, are incited somebody to action using coffee before drink is drunk Caffeine removes, the method can fast eliminating caffeine and the other active components that are lost in tealeaves, also there is operation letter Single, convenient and efficient feature.

Claims (8)

1. a kind of method of fast eliminating caffeine before drink is drunk, it is characterised in that：Specifically include following steps：
A) production of caffeine packaging bag is removed：Selection composite fibre filter membrane is material, extends side doubling, uses continuous sealer Its two sides is pressed, packaging bag is made；
B) pack of molecularly imprinted microspheres on surface：100~200 mg of molecularly imprinted microspheres on surface is taken, above-mentioned removing coffee is packed into Because of packaging bag, cotton thread is protruded into open end, they are pressed with continuous sealer and is sealed, packaging bag is placed in vacuum in polybag Packaging；
C) in drink caffeine removing：Before drink is drunk, the removing caffeine packet of molecularly imprinted microspheres on surface will be first housed It is added in drink, cotton thread extended end, to take, after 3~5 min, takes out close to rim of a cup, the removing of caffeine can be completed.
The method of fast eliminating caffeine before 2. a kind of drink according to claim 1 is drunk, it is characterised in that：Above-mentioned step It is rapid b) in, the preparation of molecularly imprinted microspheres on surface：By surface imprinted polymerization, with the modified mesoporous SiO of double bond2Microballoon is to carry Body, coffee is because of template molecule, and α-methacrylic acid is function monomer, and ethylene glycol dimethacrylate is crosslinking agent, azo Isobutyronitrile is initiator, and acetonitrile is pore-foaming agent, prepares the molecularly imprinted microspheres on surface containing template molecule, is then washed till with acetonitrile Clear liquid is transparent, again with methanol and acetic acid solution, and removing template molecule completely was dried in vacuo finally, being washed till neutrality with methanol Night obtains molecularly imprinted microspheres on surface mSiO2@MIPs。
The method of fast eliminating caffeine before 3. a kind of drink according to claim 2 is drunk, it is characterised in that：Caffeine With the molar ratio 2-3 of function monomer α-methacrylic acid：8-12；The modified mesoporous SiO of double bond2Microballoon, crosslinking agent ethylene glycol two Methacrylate, initiator azo isobutyronitrile mass ratio 10-15：20-30：4-6.
The method of fast eliminating caffeine before 4. a kind of drink according to claim 2 is drunk, it is characterised in that：On give an account of Hole SiO2The double bond modification procedure of microballoon：By mesoporous SiO2It is suspended from 3- (trimethoxy silicon substrate)-propyl methacrylate solution In, stirring, centrifuge separation is collected, and is washed, is dried in vacuo repeatedly to obtain the final product.
The method of fast eliminating caffeine before 5. a kind of drink according to claim 4 is drunk, it is characterised in that：It is mesoporous SiO2The double bond modification of microballoon is specific as follows：Using sol preparation, by mesoporous SiO2It is suspended from 3- (trimethoxy silicon substrate)-propyl In methacrylic acid ester solution, 5~8 h are stirred at 40~50 DEG C, separates and collects, washes repeatedly, 40~60 DEG C of vacuum drying are ?.
The method of fast eliminating caffeine before 6. a kind of drink according to claim 2 is drunk, it is characterised in that：Mixing is fine Dimension filter membrane bag material aperture should be less than the aperture of molecularly imprinted microspheres on surface, and the diameter of molecularly imprinted microspheres on surface is 0.3 ~0.4 μm, composite fibre filter sizes should be less than 0.15 μm.
The method of fast eliminating caffeine before 7. a kind of drink according to claim 1 is drunk, it is characterised in that：Remove coffee Coffee because packaging bag be that composite fibre filter membrane material with two layers of aperture less than 0.15 μm is made up of continuous sealer pressing.
The method of fast eliminating caffeine before 8. a kind of drink according to claim 2 is drunk, it is characterised in that：Methanol and Acetic acid solution is methanol and acetic acid according to volume ratio 9:The mixed solution of 1 cooperation.
CN201810787875.9A 2018-07-18 2018-07-18 A kind of method that drink drinks preceding fast eliminating caffeine Pending CN108887446A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201810787875.9A CN108887446A (en) 2018-07-18 2018-07-18 A kind of method that drink drinks preceding fast eliminating caffeine

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201810787875.9A CN108887446A (en) 2018-07-18 2018-07-18 A kind of method that drink drinks preceding fast eliminating caffeine

Publications (1)

Publication Number Publication Date
CN108887446A true CN108887446A (en) 2018-11-27

Family

ID=64351044

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201810787875.9A Pending CN108887446A (en) 2018-07-18 2018-07-18 A kind of method that drink drinks preceding fast eliminating caffeine

Country Status (1)

Country Link
CN (1) CN108887446A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111982833A (en) * 2020-07-31 2020-11-24 大连大学 Detection method of caffeine molecules

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0776607A1 (en) * 1995-12-07 1997-06-04 Societe Des Produits Nestle S.A. Caffeine imprint polymer
CN106932391A (en) * 2017-03-30 2017-07-07 中南林业科技大学 A kind of Test paper based on photosensitive mesoporous silicon substrate molecular engram microsphere and preparation method thereof
CN107064253A (en) * 2017-03-28 2017-08-18 深圳市赛亿科技开发有限公司 The cup of content of caffeine in a kind of detection drink
WO2017201420A1 (en) * 2016-05-19 2017-11-23 Decafino, Inc. CAFFElNE-ADSORBING MATERIAL, CAFFElNE-ADSORBING SYSTEM, DECAFFElNATION SYSTEM, AND RELATE METHODS OF REMOVING CAFFElNE FROM SOLUTIONS

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0776607A1 (en) * 1995-12-07 1997-06-04 Societe Des Produits Nestle S.A. Caffeine imprint polymer
WO2017201420A1 (en) * 2016-05-19 2017-11-23 Decafino, Inc. CAFFElNE-ADSORBING MATERIAL, CAFFElNE-ADSORBING SYSTEM, DECAFFElNATION SYSTEM, AND RELATE METHODS OF REMOVING CAFFElNE FROM SOLUTIONS
CN107064253A (en) * 2017-03-28 2017-08-18 深圳市赛亿科技开发有限公司 The cup of content of caffeine in a kind of detection drink
CN106932391A (en) * 2017-03-30 2017-07-07 中南林业科技大学 A kind of Test paper based on photosensitive mesoporous silicon substrate molecular engram microsphere and preparation method thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
傅强: "《分子印迹技术与药物分析》", 30 September 2014, 西安交通大学出版社 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111982833A (en) * 2020-07-31 2020-11-24 大连大学 Detection method of caffeine molecules
CN111982833B (en) * 2020-07-31 2023-09-08 大连大学 Detection method of caffeine molecules

Similar Documents

Publication Publication Date Title
CN101863946B (en) Method for extracting high-content mogroside V from dried momordica grosvenori swingle
CN102127125B (en) The modularization preparation of series of purification Weibull multiplexed combination purification technique and products thereof
CN110240580B (en) A method for preparing Saviae Miltiorrhizae radix extract with eutectic solvent
JP2004501757A (en) Separation method
CN104004106A (en) Method for extracting effective substances in inonotus obliquus and preparation method for teabag
CN104780912B (en) Biological degradability microballon that anticarcinogen adsorption capacity comprising albumin and dextran sulfate is improved and preparation method thereof
EP1859683A1 (en) Coffee bag
CN101270135B (en) Method for removing caffeinum from tea extract of tea polyphenol containing caffeinum
CN101386806A (en) Supercritical CO2 extraction of grape-kernel oil and method for extracting oligoprotoanthocyanidin by solvent process
CN102226211A (en) Method for preparing theaflavin by dynamic continuous oxidation on immobilized polyphenol oxidase
CN108887446A (en) A kind of method that drink drinks preceding fast eliminating caffeine
CN102120790B (en) Methods for preparing high-hydrophilcity macroporous absorption resin and removing caffeine in crude tea extracts
CN103468409A (en) Method for extracting Xinyang maojian tea extractum by utilizing subcritical technology
CN103652204A (en) Method for removing caffeine and pesticide residue in tea extract
CN108003127B (en) Method for extracting procyanidine in longan skin by using aqueous two-phase system
CN106176844A (en) The Folium Hibisci method for preparing extractive that a kind of antioxidation is strong
CN1857530A (en) Effective part of banana peel and its use
CN111393568B (en) Tocopherol molecularly imprinted polymer, preparation method and application
Pande et al. Preparation and evaluation of phytosomes of pomegrane peels
KR100367055B1 (en) Method for preparation of high functional GABA tea
CN111956717B (en) Method for preparing grape seed extract with low pesticide residue and high procyanidine
CN107549859B (en) A kind of galic essential oil compound enhances immune soft capsule and preparation method thereof
CN1709418A (en) Katsumadai seed extract and its preparation
CN101268837A (en) Method for preparing soybean oligopeptide absorbent charcoal carrier sustained-release agent
CN102786472A (en) Method for extraction separation of huperzine A in all-grass of snake foot clubmoss and its hairy root by supercritical extraction-crystallization technology

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication

Application publication date: 20181127

RJ01 Rejection of invention patent application after publication