CN108856112A - Magnetic bead cleaning method and device - Google Patents
Magnetic bead cleaning method and device Download PDFInfo
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- CN108856112A CN108856112A CN201810225779.5A CN201810225779A CN108856112A CN 108856112 A CN108856112 A CN 108856112A CN 201810225779 A CN201810225779 A CN 201810225779A CN 108856112 A CN108856112 A CN 108856112A
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- reaction cup
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- cleaning
- cleaning solution
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/04—Cleaning involving contact with liquid
- B08B3/08—Cleaning involving contact with liquid the liquid having chemical or dissolving effect
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B13/00—Accessories or details of general applicability for machines or apparatus for cleaning
Abstract
The present invention relates to sample analysis technical field, in particular to a kind of magnetic bead cleaning method and device.This method is：The cleaning solution of the first specified amount is injected into reaction cup to be cleaned；The cleaning solution of the second specified amount is injected into reaction cup to be cleaned, wherein after the cleaning solution for injecting the second specified amount, cleaning solution liquid level is not less than the extreme higher position of reaction cup wall of cup splashing liquid to be cleaned in reaction cup to be cleaned；Circulation executes n times or less and operates：Waste liquid in reaction cup to be cleaned is drawn using the aspirating needle of the double-deck cleaning needle, and the cleaning solution of third specified amount is injected into reaction cup to be cleaned using the injection needle of the double-deck cleaning needle, wherein, the fluid injection needle guard of the double-deck cleaning needle is on the outside of aspirating needle, and the liquid injection port of injection needle is higher than the liquid sucting port of aspirating needle, N is the positive integer more than or equal to 1；Waste liquid in reaction cup to be cleaned is drawn, magnetic bead cleaning is completed.
Description
Technical field
The present invention relates to sample analysis technical field, in particular to a kind of magnetic bead cleaning method and device.
Background technique
Luminescence immunoassay (Chemical Luminescence Immunity Analyzer, CLIA) is to utilize chemistry
Or instruction system of the bio-luminescence system as antigen-antibody reaction will shine so as to the method for quantitative detection antigen or antibody
Analyze and be immunoreacted a kind of novel ultramicro-analysis technology for combining and establishing.Its high sensitivity with luminesceence analysis
With the high degree of specificity of antigen-antibody reaction.Magnetic bead cleaning during CLIA is not reaction step, but determine experiment at
The key lost.The purpose of magnetic bead cleaning be wash away in reaction solution not with substance of the solid phase antigen in conjunction with antibody and reacting
Non-specific adsorption is in the interfering substance of solid phase carrier in the process.
Currently, conventional magnetic bead cleaning way there are mainly two types of mode：
First way is：Using same root cleaning needle, the filling of cleaning solution and the extraction of waste liquid are completed；The second way
For：Using two cleaning needles, the filling of cleaning solution and the extraction of waste liquid are realized respectively.
However, using above-mentioned first way, since fluid injection and drawing liquid are all made of same root cleaning needle and carry out, then, just
Cleaning needle outer wall and needle point can not be cleaned, while being also easy to produce carrying pollution, fluid injection and drawing liquid are carried out using single needle,
It needs to pump the waste liquid in pipeline when drawing liquid, in fluid injection, needs first to be filled pipeline, then carry out fluid injection, so flow
Journey is complicated, and cleaning efficiency is low, and cannot be guaranteed fluid injection precision；Using the above-mentioned second way, aspirating needle is when extracting waste liquid, meeting
It is contacted with waste liquid, waste liquid is remained in into needle point and needle wall, when carrying out conventional cleaning, there is the risk for generating and carrying pollution.Into one
Step, the reaction cup for accommodating reaction solution is being reacted, and/or is carried, and/or during mixing etc., it is possible to will be reacted
Liquid sputters at reaction cup wall of cup, generates splashing liquid, inaccurate so as to cause final experimental result.
Summary of the invention
The purpose of the embodiment of the present invention is that providing a kind of magnetic bead cleaning method and device, existed in the prior art to solve
Reaction cup wall of cup is sputtered at due to reaction solution, splashing liquid is generated, so as to cause the inaccurate problem of experimental result.
The specific technical solution provided in the embodiment of the present invention is as follows：
In a first aspect, the present invention provides a kind of magnetic bead cleaning method, which includes：To reaction cup to be cleaned
The cleaning solution of the first specified amount of middle injection；The cleaning solution of the second specified amount is injected into the reaction cup to be cleaned, wherein injecting
After the cleaning solution of second specified amount, cleaning solution liquid level is not less than the reaction cup wall of cup splashing liquid to be cleaned in the reaction cup to be cleaned
Extreme higher position；Circulation executes n times or less and operates：It is drawn using the aspirating needle of the double-deck cleaning needle useless in the reaction cup to be cleaned
Liquid, and the cleaning solution of third specified amount is injected into the reaction cup to be cleaned using the injection needle of the bilayer cleaning needle, wherein this
The fluid injection needle guard of the double-deck cleaning needle on the outside of aspirating needle, and the liquid injection port of the injection needle be higher than aspirating needle liquid sucting port, N be greater than
Positive integer equal to 1；Waste liquid in the reaction cup to be cleaned is drawn, magnetic bead cleaning is completed.
Using magnetic bead cleaning method provided by the invention, by way of secondary injection cleaning solution, so that reaction to be cleaned
Cleaning solution liquid level is not less than the extreme higher position of reaction cup wall of cup splashing liquid to be cleaned in cup, thus effectively to reaction cup wall of cup splashing liquid
It is diluted and cleans, this makes it possible to make final experimental result more accurate, further, inhaling, in injection process,
After having drawn waste liquid using the aspirating needle of the double-deck cleaning needle, infused using the injection needle of the double-deck cleaning needle into reaction cup to be cleaned
While entering cleaning solution, the waste liquid carried when aspirating needle is drawn waste liquid is poured in reaction cup to be cleaned, avoids carrying around pollution
Generation.
Optionally, the cleaning solution of the first specified amount is injected into reaction cup to be cleaned, including：Detecting taking for cleaning station
When putting station has reaction cup to be cleaned, above-mentioned reaction cup to be cleaned is rotated to the fluid injection station of above-mentioned cleaning station；And using note
Liquid needle injects the cleaning solution of the first specified amount into above-mentioned reaction cup to be cleaned.
Above-mentioned optional embodiment characterization, a kind of specific cleaning station is by the injection needle of fluid injection station to be cleaned anti-
Answer the mode that the cleaning solution of specified amount is injected in cup.
Optionally, before drawing the waste liquid in the reaction cup to be cleaned using the aspirating needle of the double-deck cleaning needle, into one
Step includes：Magnetic suck operation is carried out to the reaction cup to be cleaned；It is drawn in the aspirating needle using the double-deck cleaning needle above-mentioned to clear
It washes after the waste liquid in reaction cup, further comprises：Magnetic suck operation is released to above-mentioned reaction cup to be cleaned；Using above-mentioned double
The injection needle of layer cleaning needle is injected into above-mentioned reaction cup to be cleaned after the cleaning solution of third specified amount, further comprises：It is right
Magnetic bead in above-mentioned reaction cup to be cleaned carries out mixing operation.
Above-mentioned optional mode characterizes, reaction cup to be cleaned is inhaled, fluid injection during, can also be to new injection
The reaction cup of cleaning solution executes magnetic bead and mixes operation, specifically, releasing magnetic suck operation, and inject after having drawn waste liquid
Then new cleaning solution carries out magnetic bead and mixes operation.
Optionally, above-mentioned reaction cup to be cleaned is after the cleaning solution of injection third specified amount, and cleaning solution liquid level is not
Lower than the cleaning solution liquid level after the cleaning solution for injecting the second specified amount.
Above-mentioned optional mode characterizes, since being inhaled to reaction cup to be cleaned, fluid injection operation, to reaction to be cleaned
When injecting cleaning solution in cup, the cleaning solution liquid level being subsequently implanted into can be made to be not less than in the cleaning for injecting the second specified amount
Cleaning solution liquid level in reaction cup to be cleaned after liquid, in this way it is ensured that the subsequent cleaning to reaction cup to be cleaned is more
Thoroughly, more comprehensively, so that final experimental result is more acurrate.
Second aspect, the present invention provide a kind of magnetic bead cleaning method, which includes：To reaction cup to be cleaned
The cleaning solution of the first specified amount of middle injection；Circulation executes M following operation：It is to be cleaned to this using the injection needle of the double-deck cleaning needle
The cleaning solution of the second specified amount is injected in reaction cup, wherein described to be cleaned after the cleaning solution for injecting second specified amount
Cleaning solution liquid level is not less than the extreme higher position of the reaction cup wall of cup splashing liquid to be cleaned in reaction cup, and uses the bilayer cleaning needle
Aspirating needle draw the waste liquid in the reaction cup to be cleaned, and using the bilayer cleaning needle injection needle to the reaction to be cleaned
The cleaning solution of third specified amount is injected in cup, wherein the fluid injection needle guard of the bilayer cleaning needle is on the outside of aspirating needle, and the injection needle
Liquid injection port be higher than the liquid sucting port of aspirating needle, M is positive integer more than or equal to 1；Waste liquid in the reaction cup to be cleaned is drawn, it is complete
It is cleaned at magnetic bead.
Optionally, the reaction cup to be cleaned is after the cleaning solution of injection third specified amount, and cleaning solution liquid level is not
Higher than the cleaning solution liquid level after the cleaning solution for injecting the second specified amount.
The third aspect, the present invention provide a kind of magnetic bead cleaning device, which includes：First injection unit,
For injecting the cleaning solution of the first specified amount into reaction cup to be cleaned；Second injection unit is used for above-mentioned reaction to be cleaned
The cleaning solution of the second specified amount is injected in cup, wherein after the cleaning solution for injecting above-mentioned second specified amount, above-mentioned reaction to be cleaned
Cleaning solution liquid level is not less than the extreme higher position of above-mentioned reaction cup wall of cup splashing liquid to be cleaned in cup；Execution unit executes N for recycling
Secondary following operation：Waste liquid in above-mentioned reaction cup to be cleaned is drawn using the aspirating needle of the double-deck cleaning needle, and uses above-mentioned bilayer
The injection needle of cleaning needle injects the cleaning solution of third specified amount into above-mentioned reaction cup to be cleaned, wherein above-mentioned bilayer cleaning needle
Fluid injection needle guard on the outside of aspirating needle, and the liquid injection port of above-mentioned injection needle be higher than aspirating needle liquid sucting port, N be more than or equal to 1
Positive integer；Suction unit completes magnetic bead cleaning for drawing waste liquid in above-mentioned reaction cup to be cleaned.
Optionally, when injecting the cleaning solution of the first specified amount into reaction cup to be cleaned, above-mentioned first injection unit is used
In：When the pick-and-place station for detecting cleaning station has reaction cup to be cleaned, above-mentioned reaction cup to be cleaned is rotated to above-mentioned cleaning
The fluid injection station stood；And the cleaning solution of the first specified amount is injected into above-mentioned reaction cup to be cleaned using injection needle.
Optionally, before drawing the waste liquid in the reaction cup to be cleaned using the aspirating needle of the double-deck cleaning needle, into one
Step includes：Magnetic suck operation is carried out to the reaction cup to be cleaned；It is drawn in the aspirating needle using the double-deck cleaning needle above-mentioned to clear
It washes after the waste liquid in reaction cup, above-mentioned execution unit is further used for：Magnetic suck operation is released to above-mentioned reaction cup to be cleaned；
After injecting the cleaning solution of third specified amount into above-mentioned reaction cup to be cleaned using the injection needle of the above-mentioned double-deck cleaning needle, on
Execution unit is stated to be further used for：Mixing operation is carried out to the magnetic bead in above-mentioned reaction cup to be cleaned.
Optionally, above-mentioned reaction cup to be cleaned is after the cleaning solution of injection third specified amount, and cleaning solution liquid level is not
Lower than the cleaning solution liquid level after the cleaning solution for injecting the second specified amount.
Fourth aspect, the present invention provide a kind of magnetic bead cleaning device, which includes：Injection unit is used for
The cleaning solution of the first specified amount is injected into reaction cup to be cleaned；Execution unit executes M following operation for recycling：Using
The injection needle of the double-deck cleaning needle injects the cleaning solution of the second specified amount into the reaction cup to be cleaned, wherein is injecting described the
After the cleaning solution of two specified amounts, cleaning solution liquid level is not less than the reaction cup wall of cup splashing liquid to be cleaned in the reaction cup to be cleaned
Extreme higher position, and the waste liquid in the reaction cup to be cleaned is drawn using the aspirating needle of the bilayer cleaning needle, and use this pair
The injection needle of layer cleaning needle injects the cleaning solution of third specified amount into the reaction cup to be cleaned, wherein the bilayer cleaning needle
Fluid injection needle guard is on the outside of aspirating needle, and the liquid injection port of the injection needle is higher than the liquid sucting port of aspirating needle, and M is just whole more than or equal to 1
Number；Suction unit completes magnetic bead cleaning for drawing waste liquid in the reaction cup to be cleaned.
Optionally, the reaction cup to be cleaned is after the cleaning solution of injection third specified amount, and cleaning solution liquid level is not
Higher than the cleaning solution liquid level after the cleaning solution for injecting the second specified amount.
5th aspect, the present invention provide a kind of calculating equipment, which includes：Memory refers to for storing program
It enables；Processor executes in above-mentioned first aspect for calling the program instruction stored in above-mentioned memory according to the program of acquisition
Any one method.
6th aspect, the present invention provide a kind of computer readable storage medium, which has
Computer executable instructions, above-mentioned computer executable instructions are for making above-mentioned computer execute any one of above-mentioned first aspect
Method.
The present invention has the beneficial effect that：
In conclusion using magnetic bead cleaning method provided by the invention, made by way of secondary injection cleaning solution to
The extreme higher position that cleaning solution liquid level in reaction cup is not less than reaction cup wall of cup splashing liquid is cleaned, thus effectively to reaction cup cup
Wall splashing liquid is diluted and cleans, so that final experimental result is more accurate, and is inhaling, in injection process, using double-deck clear
Wash needle aspirating needle drawn waste liquid after, cleaning solution is injected into reaction cup to be cleaned using the injection needle of the double-deck cleaning needle
Meanwhile the waste liquid carried when aspirating needle is drawn waste liquid pours in reaction cup to be cleaned, avoids carrying around the generation of pollution.
Detailed description of the invention
Fig. 1 is the detail flowchart of the first magnetic bead cleaning method in the embodiment of the present invention；
Fig. 2 is a kind of structural schematic diagram of cleaning station in the embodiment of the present invention；
Fig. 3 is a kind of structural schematic diagram of bilayer cleaning needle in the embodiment of the present invention；
Fig. 4 is the detail flowchart of second of magnetic bead cleaning method in the embodiment of the present invention；
Fig. 5 is the detail flowchart of the third magnetic bead cleaning method in the embodiment of the present invention；
Fig. 6 is the detail flowchart of the 4th kind of magnetic bead cleaning method in the embodiment of the present invention；
Fig. 7 is a kind of structural schematic diagram of magnetic bead cleaning device in the embodiment of the present invention；
Fig. 8 is the structural schematic diagram of another magnetic bead cleaning device in the embodiment of the present invention.
Specific embodiment
The technical solution introduced in embodiment to facilitate the understanding of the present invention now provides the definition of part term：
1, magnetic bead cleans, refer to washing away in reaction solution not with substance of the solid phase antigen in conjunction with antibody and reacting
Non-specific adsorption is in the method for the interfering substance of solid phase carrier in the process.
2, magnetic suck operates, and refers to making by magnetic absorbing unit magnetic bead to be adsorbed on corresponding with magnetic absorbing unit anti-
Answer the operation on glass wall of cup.
3, injection needle refers to the single cleaning needle for being served only for fluid injection operation；Aspirating needle refers to being served only for imbibition operation
Single cleaning needle.
4, the double-deck cleaning needle refers to including injection needle and aspirating needle, can be not only used for fluid injection operation, also can be used for imbibition behaviour
The cleaning needle of work.
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, is not whole embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Firstly, term "and" in the embodiment of the present invention, a kind of only incidence relation for describing affiliated partner, expression can be with
There are three kinds of relationships, for example, A and B, can indicate：Individualism A exists simultaneously A and B, these three situations of individualism B.Separately
Outside, character "/" herein typicallys represent the relationship that forward-backward correlation object is a kind of "or".
When the present invention refers to ordinal numbers such as " first ", " second ", " third " or " the 4th ", unless based on context its
The meaning of certain order of representation, it is appreciated that being only to distinguish to be used.
The solution of the present invention will be described in detail by specific embodiment below, certainly, the present invention is not limited to
Lower embodiment.
As shown in fig.1, a kind of detailed process of magnetic bead cleaning method is as follows in the embodiment of the present invention：
Step 100：The cleaning solution of the first specified amount is injected into reaction cup to be cleaned.
Specifically, having in the pick-and-place station for detecting cleaning station to be cleaned in the embodiment of the present invention when executing step 100
When reaction cup, the reaction cup is rotated to the fluid injection station of the cleaning station；And use injection needle to the reaction to be cleaned
The cleaning solution of the first specified amount is injected in cup.
In practical application, the reaction by cleaning station to magnetic bead and reaction solution is accommodated can be understood as to the cleaning of magnetic bead
The cleaning of cup, cleaning station includes multiple work stations, can be by rotating so that reaction cup to be cleaned is rotated to different operating station
On.
Illustratively, as shown in fig.2, the structural schematic diagram of rotatable cleaning station.The cleaning station may include picking and placing work
Position and several imbibitions, fluid injection, and/or inhale, fluid injection station (e.g., fluid injection station, the first suction, fluid injection station, the second suction, fluid injection work
Position, third suction liquid station and imbibition station).
In the embodiment of the present invention, so-called pick-and-place station refers to for reaction cup to be cleaned being placed on rinsing table, Yi Jicong
The position for the reaction cup cleaned is removed on rinsing table；So-called fluid injection station refers to injecting into reaction cup to be cleaned clear
The station of washing lotion；So-called imbibition station refers to the station that waste liquid is drawn from reaction cup to be cleaned；So-called suction, fluid injection station,
It refers to that waste liquid can be drawn from reaction cup to be cleaned, the station of cleaning solution can be also injected into reaction cup to be cleaned.
In the embodiment of the present invention, when cleaning station, which detects, has reaction cup to be cleaned on pick-and-place station, by reaction to be cleaned
Cup is rotated to fluid injection station, and using the cleaning needle that the fluid injection station is arranged in, is injected into reaction cup to be cleaned a certain amount of
Cleaning solution, to dilute reaction solution in reaction cup to be cleaned.In practical application, injected into reaction cup to be cleaned clearly in first time
When washing lotion, the injection rate of cleaning solution will not be too many, it is believed that after completing the injection of first time cleaning solution, cleaning solution liquid level
The extreme higher position of reaction cup wall of cup splashing liquid can not be covered.
Certainly, above-mentioned cleaning needle can be the injection needle for only having liquid injecting function, be also possible in the embodiment of the present invention i.e.
The double-deck cleaning needle referred to.Illustratively, as shown in fig.3, the structural schematic diagram of the double-deck cleaning needle.The bilayer cleaning needle is inhaled
Liquid needle 1 and injection needle 2, aspirating needle 1 and injection needle 2 are mutually not turned on, and injection needle 2 is connected to liquid injection port 4, will be inhaled by guide sleeve 3
Liquid needle 1, injection needle 2 and liquid injection port 4 link together, and 2 sets of injection needle of the bilayer cleaning needle are on the outside of aspirating needle 1, and fluid injection
The liquid injection port of needle 2 is higher than the liquid sucting port of aspirating needle 1.
In practical application, aspirating needle 1 when drawing waste liquid, can be contacted with waste liquid, then, aspirating needle 1 draw waste liquid it
Afterwards, outside and liquid sucting port can carry waste liquid, in this way, when carrying out imbibition to next reaction cup to be cleaned, can to it is next to
It cleans reaction cup and generates to carry and pollute, and the double-deck cleaning needle provided by the invention is used to carry out imbibition and fluid injection, due to the bilayer
2 sets of the injection needle outsides in aspirating needle 1 of cleaning needle, and the liquid injection port of injection needle 2 is higher than the liquid sucting port of aspirating needle 1, then,
It is during injection needle 2 reinjects cleaning solution, the waste liquid carried on aspirating needle 1 is again heavy after aspirating needle 1 completes waste liquid absorption
It newly rinses in current reaction cup to be cleaned, to avoid the hair of carrying pollution of the aspirating needle 1 to subsequent reaction cup to be cleaned
It is raw.
Step 110：The cleaning solution of the second specified amount is injected into above-mentioned reaction cup to be cleaned, wherein injecting above-mentioned the
After the cleaning solution of two specified amounts, cleaning solution liquid level is not less than above-mentioned reaction cup wall of cup splashing liquid to be cleaned in above-mentioned reaction cup to be cleaned
Extreme higher position.
Specifically, when executing step 110, being infused in fluid injection station into reaction cup to be cleaned in the embodiment of the present invention
After entering a certain amount of cleaning solution, by reaction cup to be cleaned rotate to next station (can be fluid injection station, be also possible to inhale,
Fluid injection station), and a certain amount of cleaning solution is re-injected into reaction cup to be cleaned, to ensure cleaning solution in reaction cup to be cleaned
Liquid level is not less than the extreme higher position of reaction cup wall of cup splashing liquid to be cleaned, to realize through cleaning solution to reaction cup to be cleaned
Wall of cup splashing liquid is diluted and/or cleans.
If above-mentioned next station is fluid injection station, using the cleaning needle that the fluid injection station is arranged in, to reaction to be cleaned
A certain amount of cleaning solution is injected in cup；If above-mentioned next station is suction, fluid injection station, using setting in the suction, fluid injection station
The double-deck cleaning needle injection needle, a certain amount of cleaning solution is injected into reaction cup to be cleaned.
In practical application, different types of reaction cup and/or different types of luminescence immunoassay, and/or answered in difference
With under scene, occurring the case where reaction cup wall of cup splashing liquid in reaction process, handling process or blending process, in reaction cup
Also it is not quite similar, then, the extreme higher position of the reaction cup wall of cup splashing liquid under different situations can be determined by many experiments,
And then it can be determined according to first time cleaning solution injection rate and the extreme higher position by testing obtained reaction cup wall of cup splashing liquid
Second of cleaning solution injection rate.
Step 120：Waste liquid in above-mentioned reaction cup to be cleaned is drawn using the aspirating needle of the double-deck cleaning needle, and using above-mentioned
The injection needle of the double-deck cleaning needle injects the cleaning solution of third specified amount into above-mentioned reaction cup to be cleaned.
In practical application, generally in drawing reaction cup to be cleaned before waste liquid, need to carry out magnetic to reaction cup to be cleaned
Adsorption operations, so that magnetic bead is adsorbed on wall of cup, to carry out the absorption of waste liquid.
Specifically, in the embodiment of the present invention, if the station that is currently located of reaction cup to be cleaned is fluid injection station, then, i.e.,
Reaction cup to be cleaned need to be rotated to inhale, fluid injection station, carry out magnetic suck operation, and using the suction, fluid injection station is arranged in
The aspirating needle of the double-deck cleaning needle draws the waste liquid in reaction cup to be cleaned, and after completion of the aspiration, using the bilayer cleaning needle
Injection needle injects a certain amount of cleaning solution into reaction cup to be cleaned, completes primary suction, fluid injection operation.
If reaction cup to be cleaned is currently located station as suction, fluid injection station, magnetic suck operation is carried out, and directly adopt
The suction is set, the aspirating needle of the double-deck cleaning needle of fluid injection station is drawn waste liquid in reaction cup to be cleaned, and completed drawing
Afterwards, a certain amount of cleaning solution is injected into reaction cup to be cleaned using the injection needle of the bilayer cleaning needle, completes primary suction, fluid injection
Operation.
Further, in the embodiment of the present invention, a kind of preferable embodiment is, in the aspirating needle using the double-deck cleaning needle
Before drawing the waste liquid in above-mentioned reaction cup to be cleaned, magnetic suck operation is carried out to above-mentioned reaction cup to be cleaned；Using double-deck
After the aspirating needle of cleaning needle draws the waste liquid in above-mentioned reaction cup to be cleaned, magnetic suck behaviour is released to above-mentioned reaction cup to be cleaned
Make；And the injection needle using the above-mentioned double-deck cleaning needle injected into above-mentioned reaction cup to be cleaned third specified amount cleaning solution it
Afterwards, mixing operation is carried out to the magnetic bead in above-mentioned reaction cup to be cleaned.That is, being inhaled at one, being primary complete on fluid injection station
Whole suction, fluid injection operation may include：1, magnetic suck operates, 2, waste liquid absorption, 3, magnetic suck operation releasing, 4, cleaning solution note
Enter, 5, magnetic bead mixing.
Certainly, it is inhaled at one, fluid injection station can be only performed once suction, fluid injection operation, also executable repeatedly suction, fluid injection behaviour
Make；Cleaning station can only include suction, a fluid injection station, may also comprise multiple suctions, fluid injection station；Each suction, fluid injection station execute
Suction, fluid injection number of operations may be the same or different, it is each to inhale, magnetic bead be executed in fluid injection operation mix operation, can also
Operation is mixed not execute magnetic bead.Specific applicable cases can accordingly be set according to practical application scene and/or user demand
It sets.
Step 130：Judge that cleaning needle inhales above-mentioned reaction cup to be cleaned to above-mentioned use bilayer, whether fluid injection number reaches
To given threshold, if so, thening follow the steps 140；Otherwise, step 120 is executed.
Step 140：Waste liquid in above-mentioned reaction cup to be cleaned is drawn, magnetic bead cleaning is completed.
It in the embodiment of the present invention, needs to draw waste liquid in reaction cup to be cleaned, with the magnetic bead after being cleaned, thus complete
The cleaning of pairs of magnetic bead.
In practical application, if being provided with corresponding imbibition station on cleaning station, last time suction, fluid injection operation are completed
Later, reaction cup to be cleaned is rotated to imbibition station, carries out magnetic suck operation, and using the imbibition that the imbibition station is arranged in
Needle draws the waste liquid in reaction cup to be cleaned, and the reaction cup after cleaning is rotated to station is picked and placed, thus after obtaining cleaning
Magnetic bead.
If being not provided with corresponding imbibition station on cleaning station, inhaled, after fluid injection operation completing last time, directly
Using be arranged in be currently located suction, the aspirating needle of the double-deck cleaning needle of fluid injection station draws waste liquid in reaction cup to be cleaned, and
Reaction cup after cleaning is rotated to station is picked and placed, thus the magnetic bead after being cleaned.
Illustratively, as shown in fig.4, in the embodiment of the present invention, a kind of detailed process of magnetic bead cleaning method is as follows：
Step 400：The cleaning solution of the first specified amount is injected into reaction cup to be cleaned.
Step 410：Circulation executes M following operation：Using the injection needle of the double-deck cleaning needle to above-mentioned reaction cup to be cleaned
The cleaning solution of the second specified amount of middle injection, wherein after the cleaning solution for injecting above-mentioned second specified amount, above-mentioned reaction cup to be cleaned
Middle cleaning solution liquid level is not less than the extreme higher position of above-mentioned reaction cup wall of cup splashing liquid to be cleaned, and using the suction of the above-mentioned double-deck cleaning needle
Liquid needle draws the waste liquid in above-mentioned reaction cup to be cleaned, and using the above-mentioned double-deck cleaning needle injection needle to above-mentioned to be cleaned anti-
Answer the cleaning solution that third specified amount is injected in cup.
In practical application, if next station of fluid injection station is the first suction, fluid injection station, when reaction cup to be cleaned operates
It is inhaled, after fluid injection station to above-mentioned first, using the injection needle for the first double-deck cleaning needle that above-mentioned first suction, fluid injection station is arranged in
A certain amount of cleaning solution is injected, into reaction cup to be cleaned to ensure that cleaning solution page height is not less than cup in reaction cup to be cleaned
The extreme higher position of wall splashing liquid carries out magnetic suck operation, and to be cleaned using the aspirating needle absorption of the first double-deck cleaning needle later
Waste liquid in reaction cup is further injected centainly using the injection needle of the first double-deck cleaning needle into reaction cup to be cleaned
The cleaning solution of amount, so that the waste liquid carried when the aspirating needle of the first double-deck cleaning needle is drawn waste liquid pours reaction cup to be cleaned
In；It is inhaled, after the secondary fluid injection of fluid injection station completion first, reaction cup to be cleaned is rotated to next station, is executed subsequent
Magnetic bead cleaning process.
If next station is the second suction, fluid injection station, it can choose and magnetic suck operation is released to reaction cup to be cleaned, and
It is injected using the injection needle of the second suction, fluid injection station is arranged in the second double-deck cleaning needle into reaction cup to be cleaned a certain amount of
Cleaning solution carries out magnetic suck operation, and draw in reaction cup to be cleaned using the aspirating needle of the second double-deck cleaning needle later
Waste liquid further injects a certain amount of cleaning solution into reaction cup to be cleaned using the injection needle of the second double-deck cleaning needle,
So that the waste liquid carried when the aspirating needle of the second double-deck cleaning needle is drawn waste liquid pours in reaction cup to be cleaned；Second
It inhales, after the secondary fluid injection of fluid injection station completion, reaction cup to be cleaned is rotated to next station, execute subsequent magnetic bead cleaning stream
Journey.
It certainly, can also include a mixing between two suctions of arbitrary neighborhood, fluid injection station in the embodiment of the present invention
Station can carry out mixing behaviour to the magnetic bead in above-mentioned reaction cup to be cleaned by the evenly mixing device being arranged on the mixing station
Make.
Further, a kind of preferable embodiment is in the embodiment of the present invention, and above-mentioned reaction cup to be cleaned is in injection the
After the cleaning solution of three specified amounts, cleaning solution liquid level is not higher than the cleaning solution liquid after the cleaning solution for injecting the second specified amount
Face height.In this way it is ensured that reaction cup to be cleaned is from a station during operating to next station, reaction to be cleaned
Cleaning solution in cup does not overflow.
Step 420：Waste liquid in above-mentioned reaction cup to be cleaned is drawn, magnetic bead cleaning is completed.
It, will be to be cleaned anti-using the aspirating needle that the imbibition station is arranged in if reaction cup to be cleaned is rotated to imbibition station
The waste liquid in cup is answered to draw, the magnetic bead after obtaining cleaning completes magnetic bead cleaning.
Above-described embodiment is described in further detail using specific application scenarios below, as shown in fig.5, of the invention
In embodiment, a kind of detailed process of magnetic bead cleaning method is as follows：
Step 500：Reaction cup to be cleaned is operated to pick-and-place station.
Specifically, reaction cup to be cleaned can be grabbed to the pick-and-place station of cleaning station using reaction cup grasping mechanism.
Step 501：Judge to pick and place whether station has reaction cup to be cleaned, if so, thening follow the steps 502；Otherwise, step is executed
Rapid 510.
Specifically, cleaning station, which can detecte, picks and places whether be placed with reaction cup to be cleaned on station, and detecting
When reaction cup to be cleaned, subsequent cleaning operation is continued to execute；If reaction cup to be cleaned has been not detected, sound an alarm and/or
It shuts down.
Step 502：Reaction cup to be cleaned is rotated to fluid injection station.
Specifically, cleaning station detects that picking and placing station has reaction cup to be cleaned, then rotary-cleaning station, so that reaction to be cleaned
Cup is rotated to fluid injection station.
Step 503：Cleaning solution is injected into reaction cup to be cleaned using injection needle.
Specifically, cleaning station injects the first specified amount into reaction cup to be cleaned using the injection needle that fluid injection station is arranged in
Cleaning solution.
Step 504：Reaction cup to be cleaned is rotated to suction, fluid injection station, and magnetic suck behaviour is carried out to reaction cup to be cleaned
Make.
Specifically, cleaning station is completed after the first time fluid injection of fluid injection station, reaction cup to be cleaned is rotated to suction, note
Liquid station, and start magnetic absorbing unit (e.g., magnet, electromagnet etc.) and magnetic suck operation is carried out to reaction cup to be cleaned, wherein it is every
One inhales, fluid injection station is both provided with the double-deck cleaning needle that can be used for imbibition and fluid injection accordingly, and the aspirating needle of the double-deck cleaning needle can
For drawing the waste liquid in reaction cup to be cleaned, the injection needle of the double-deck cleaning needle can be used for injecting cleaning into reaction cup to be cleaned
Liquid.
Step 505：A certain amount of cleaning solution is injected into reaction cup to be cleaned using the injection needle of the double-deck cleaning needle, so that
Cleaning solution liquid level is not less than the extreme higher position of wall of cup splashing liquid.
Specifically, to reaction cup to be cleaned carry out magnetic suck operation after, using the double-deck cleaning needle injection needle to
The cleaning solution that the second specified amount is injected in cleaning reaction cup, so that cleaning solution liquid level is splashed not less than wall of cup in reaction cup to be cleaned
The extreme higher position of liquid, to realize the dilution and cleaning to wall of cup splashing liquid in reaction cup to be cleaned.
Step 506：Waste liquid in reaction cup to be cleaned is drawn using the aspirating needle of the double-deck cleaning needle, and using the double-deck cleaning
The injection needle of needle injects a certain amount of cleaning solution into reaction cup to be cleaned.
Specifically, in the embodiment of the present invention, since the fluid injection needle guard of the double-deck cleaning needle is on the outside of aspirating needle, and injection needle
Liquid injection port is higher than the liquid sucting port of aspirating needle, then, waste liquid and then use injection needle are being drawn to be cleaned using aspirating needle
Cleaning solution is injected in reaction cup, so that the waste-liquid cleaning carried on aspirating needle goes down, avoids the waste liquid that will be carried on aspirating needle
It carries into next reaction cup to be cleaned.
Step 507：Judge whether reach in the number inhaled, fluid injection station inhales reaction cup to be cleaned, fluid injection operates
Threshold value, if so, thening follow the steps 508；Otherwise, step 506 is executed.
Specifically, corresponding threshold value can be arranged according to different application scene and/or different user demands, for example, it is assumed that threshold
Value is N, then is recycled and executes n times suction, fluid injection operation, wherein N is the positive integer more than or equal to 1.
Step 508：Reaction cup to be cleaned is rotated to imbibition station, is drawn using aspirating needle useless in reaction cup to be cleaned
Liquid.
Specifically, cleaning station is after determining to complete to operate the suction of reaction cup to be cleaned, fluid injection, by reaction cup to be cleaned
It rotates to imbibition station, and the waste liquid in reaction cup to be cleaned is drawn using the aspirating needle that imbibition station is arranged in, at this point, obtaining
Reaction cup after cleaning accommodates the magnetic bead after cleaning in reaction cup after cleaning.
Step 509：Reaction cup after cleaning is rotated to station is picked and placed, magnetic bead cleaning is completed.
Specifically, cleaning station rotates reaction cup to be cleaned to station is picked and placed, the reaction cup after can taking cleaning away completes magnetic
Pearl cleaning process.
Step 510：Alarm and/or shutdown.
Further, as shown in connection with fig. 2 cleaning station and magnetic bead cleaning process shown in fig. 5 complete a magnetic bead cleaning
Process include：Reaction cup to be cleaned is placed on cleaning station and picks and places station；Reaction cup to be cleaned is rotated to fluid injection station, and
The cleaning solution of the first specified amount is injected into reaction cup to be cleaned using injection needle；By reaction cup to be cleaned rotate to first inhale,
Fluid injection station injects the cleaning solution of the second specified amount using the injection needle of the first double-deck cleaning needle into reaction cup to be cleaned, and
Magnetic suck operation is carried out to reaction cup to be cleaned, and is drawn in reaction cup to be cleaned using the aspirating needle of the first double-deck cleaning needle
Waste liquid the is injected into reaction cup to be cleaned using the injection needle of the first double-deck cleaning needle immediately after having drawn waste liquid
The cleaning solution of three specified amounts；Reaction cup to be cleaned is rotated to the second suction, fluid injection station, magnetic suck is carried out to reaction cup to be cleaned
Operation is drawn the waste liquid in reaction cup to be cleaned using the aspirating needle of the second double-deck cleaning needle, and after having drawn waste liquid, is stood
The cleaning solution of the 4th specified amount is injected into reaction cup to be cleaned using the injection needle of the second double-deck cleaning needle；It will be to be cleaned anti-
It answers cup to rotate to third suction, fluid injection station, magnetic suck operation is carried out to reaction cup to be cleaned, using the suction of third bilayer cleaning needle
Liquid needle draws the waste liquid in reaction cup to be cleaned, and after having drawn waste liquid, uses the fluid injection of third bilayer cleaning needle immediately
Needle injects the cleaning solution of the 4th specified amount into reaction cup to be cleaned；Reaction cup to be cleaned is rotated to imbibition station, and is used
Aspirating needle draws the waste liquid in reaction cup to be cleaned；Reaction cup after cleaning is rotated to station is picked and placed, is taken away anti-after cleaning
Cup is answered, the magnetic bead after being cleaned completes magnetic bead cleaning.It is of course also possible to mix behaviour inhaling, magnetic bead being added in injection process
Make.
Above-described embodiment is described in further detail using specific application scenarios below, as shown in fig.6, of the invention
In embodiment, a kind of detailed process of magnetic bead cleaning method is as follows：
Step 600：Reaction cup to be cleaned is operated to pick-and-place station.
Step 601：Judge to pick and place whether station has reaction cup to be cleaned, if so, thening follow the steps 602；Otherwise, step is executed
Rapid 609.
Step 602：Reaction cup to be cleaned is rotated to fluid injection station.
Step 603：Cleaning solution is injected into reaction cup to be cleaned using injection needle.
Step 604：Reaction cup to be cleaned is rotated to suction, fluid injection station, using the injection needle of the double-deck cleaning needle to clear
It washes in reaction cup and injects a certain amount of cleaning solution, so that cleaning solution liquid level is not less than the extreme higher position of wall of cup splashing liquid.
Step 605：Magnetic suck operation is carried out to reaction cup to be cleaned, is drawn using the aspirating needle of the double-deck cleaning needle to be cleaned
Waste liquid in reaction cup, and a certain amount of cleaning solution is injected into reaction cup to be cleaned using the injection needle of the double-deck cleaning needle.
Step 606：Judge whether reach in the number inhaled, fluid injection station inhales reaction cup to be cleaned, fluid injection operates
Threshold value, if so, thening follow the steps 607；Otherwise, step 604 is executed.
Step 607：Reaction cup to be cleaned is rotated to imbibition station, is drawn using aspirating needle useless in reaction cup to be cleaned
Liquid.
Step 608：Reaction cup after cleaning is rotated to station is picked and placed, magnetic bead cleaning is completed.
Step 609：Alarm and/or shutdown.
Further, as shown in connection with fig. 2 cleaning station and magnetic bead cleaning process shown in fig. 6 complete a magnetic bead cleaning
Process include：Reaction cup to be cleaned is placed on cleaning station and picks and places station；Reaction cup to be cleaned is rotated to fluid injection station, and
The cleaning solution of the first specified amount is injected into reaction cup to be cleaned using injection needle；By reaction cup to be cleaned rotate to first inhale,
Fluid injection station injects the cleaning solution of the second specified amount using the injection needle of the first double-deck cleaning needle into reaction cup to be cleaned, and
Magnetic suck operation is carried out to reaction cup to be cleaned, and is drawn in reaction cup to be cleaned using the aspirating needle of the first double-deck cleaning needle
Waste liquid the is injected into reaction cup to be cleaned using the injection needle of the first double-deck cleaning needle immediately after having drawn waste liquid
The cleaning solution of three specified amounts；Reaction cup to be cleaned is rotated to the second suction, fluid injection station, using the fluid injection of the second double-deck cleaning needle
Needle injects the cleaning solution of the 4th specified amount into reaction cup to be cleaned, magnetic suck operation is carried out to reaction cup to be cleaned, using the
The aspirating needle of the two double-deck cleaning needles draws the waste liquid in reaction cup to be cleaned, and after having drawn waste liquid, uses second immediately
The injection needle of the double-deck cleaning needle injects the cleaning solution of third specified amount into reaction cup to be cleaned；By reaction cup to be cleaned rotate to
Third suction, fluid injection station, inject the clear of the 4th specified amount into reaction cup to be cleaned using the injection needle of third bilayer cleaning needle
Washing lotion carries out magnetic suck operation to reaction cup to be cleaned, draws reaction cup to be cleaned using the aspirating needle of third bilayer cleaning needle
In waste liquid infused immediately using the injection needle of third bilayer cleaning needle into reaction cup to be cleaned and after having drawn waste liquid
Enter the cleaning solution of third specified amount；Reaction cup to be cleaned is rotated to imbibition station, and reaction to be cleaned is drawn using aspirating needle
Waste liquid in cup；Reaction cup after cleaning is rotated to station is picked and placed, takes the reaction cup after cleaning, the magnetic after being cleaned away
Pearl completes magnetic bead cleaning.It is of course also possible to mix operation inhaling, magnetic bead being added in injection process.
Based on the above embodiment, as shown in fig.7, in the embodiment of the present invention, a kind of magnetic bead cleaning device includes at least the
One injection unit 70, the second injection unit 71, execution unit 72 and suction unit 73, wherein
First injection unit 70, for injecting the cleaning solution of the first specified amount into reaction cup to be cleaned；
Second injection unit 71, for injecting the cleaning solution of the second specified amount into the reaction cup to be cleaned, wherein
After injecting the cleaning solution of second specified amount, cleaning solution liquid level is not less than the reaction to be cleaned in the reaction cup to be cleaned
The extreme higher position of cup wall of cup splashing liquid；
Execution unit 72 is operated for recycling execution n times or less：It is drawn using the aspirating needle of the double-deck cleaning needle described to clear
The waste liquid in reaction cup is washed, and third is injected into the reaction cup to be cleaned using the injection needle of the double-deck cleaning needle and is specified
The cleaning solution of amount, wherein the fluid injection needle guard of the bilayer cleaning needle is on the outside of aspirating needle, and the liquid injection port of the injection needle is higher than
The liquid sucting port of aspirating needle, N are the positive integer more than or equal to 1；
Suction unit 73 completes magnetic bead cleaning for drawing waste liquid in the reaction cup to be cleaned.
Optionally, when injecting the cleaning solution of the first specified amount into reaction cup to be cleaned, first injection unit 70
For：
When the pick-and-place station for detecting cleaning station has reaction cup to be cleaned, the reaction cup to be cleaned is rotated to described
The fluid injection station of cleaning station；And
The cleaning solution of the first specified amount is injected into the reaction cup to be cleaned using injection needle.
Optionally, described before drawing the waste liquid in the reaction cup to be cleaned using the aspirating needle of the double-deck cleaning needle
Execution unit 72 is further used for：Magnetic suck operation is carried out to the reaction cup to be cleaned；
After drawing the waste liquid in the reaction cup to be cleaned using the aspirating needle of the double-deck cleaning needle, the execution unit
72 are further used for：Magnetic suck operation is released to the reaction cup to be cleaned；
The cleaning of third specified amount is injected into the reaction cup to be cleaned in the injection needle using the double-deck cleaning needle
After liquid, the execution unit 72 is further used for：Mixing operation is carried out to the magnetic bead in the reaction cup to be cleaned.
Optionally, the reaction cup to be cleaned is after the cleaning solution of injection third specified amount, and cleaning solution liquid level is not
Lower than the cleaning solution liquid level after the cleaning solution for injecting the second specified amount.
Illustratively, as shown in fig.8, in the embodiment of the present invention, a kind of magnetic bead cleaning device includes at least injection unit
80, execution unit 81 and suction unit 82, wherein
Injection unit 80, for injecting the cleaning solution of the first specified amount into reaction cup to be cleaned；
Execution unit 81 executes M following operation for recycling：Using the injection needle of the double-deck cleaning needle to described to be cleaned
The cleaning solution of the second specified amount is injected in reaction cup, wherein described to be cleaned after the cleaning solution for injecting second specified amount
Cleaning solution liquid level is not less than the extreme higher position of the reaction cup wall of cup splashing liquid to be cleaned in reaction cup, and using the double-deck cleaning
The aspirating needle of needle draws the waste liquid in the reaction cup to be cleaned, and using the double-deck cleaning needle injection needle to it is described to
Clean reaction cup in inject third specified amount cleaning solution, wherein it is described bilayer cleaning needle fluid injection needle guard on the outside of aspirating needle,
And the liquid injection port of the injection needle is higher than the liquid sucting port of aspirating needle, M is the positive integer more than or equal to 1；
Suction unit 82 completes magnetic bead cleaning for drawing waste liquid in the reaction cup to be cleaned.
Optionally, the reaction cup to be cleaned is after the cleaning solution of injection third specified amount, and cleaning solution liquid level is not
Higher than the cleaning solution liquid level after the cleaning solution for injecting the second specified amount.
In conclusion using magnetic bead cleaning method provided by the invention, made by way of secondary injection cleaning solution to
The extreme higher position that cleaning solution liquid level in reaction cup is not less than reaction cup wall of cup splashing liquid is cleaned, thus effectively to reaction cup cup
Wall splashing liquid is diluted and cleans, so that final experimental result is more accurate, and is inhaling, in injection process, using double-deck clear
Wash needle aspirating needle drawn waste liquid after, cleaning solution is injected into reaction cup to be cleaned using the injection needle of the double-deck cleaning needle
Meanwhile the waste liquid carried when aspirating needle is drawn waste liquid pours in reaction cup to be cleaned, avoids carrying around the generation of pollution.
It should be understood by those skilled in the art that, the embodiment of the present invention can provide as method, system or computer program
Product.Therefore, complete hardware embodiment, complete software embodiment or reality combining software and hardware aspects can be used in the present invention
Apply the form of example.Moreover, it wherein includes the computer of computer usable program code that the present invention, which can be used in one or more,
The computer program implemented in usable storage medium (including but not limited to magnetic disk storage, CD-ROM, optical memory etc.) produces
The form of product.
The present invention be referring to according to the method for the embodiment of the present invention, the process of equipment (system) and computer program product
Figure and/or block diagram describe.It should be understood that every one stream in flowchart and/or the block diagram can be realized by computer program instructions
The combination of process and/or box in journey and/or box and flowchart and/or the block diagram.It can provide these computer programs
Instruct the processor of general purpose computer, special purpose computer, Embedded Processor or other programmable data processing devices to produce
A raw machine, so that being generated by the instruction that computer or the processor of other programmable data processing devices execute for real
The device for the function of being specified in present one or more flows of the flowchart and/or one or more blocks of the block diagram.
These computer program instructions, which may also be stored in, is able to guide computer or other programmable data processing devices with spy
Determine in the computer-readable memory that mode works, so that it includes referring to that instruction stored in the computer readable memory, which generates,
Enable the manufacture of device, the command device realize in one box of one or more flows of the flowchart and/or block diagram or
The function of being specified in multiple boxes.
These computer program instructions also can be loaded onto a computer or other programmable data processing device, so that counting
Series of operation steps are executed on calculation machine or other programmable devices to generate computer implemented processing, thus in computer or
The instruction executed on other programmable devices is provided for realizing in one or more flows of the flowchart and/or block diagram one
The step of function of being specified in a box or multiple boxes.
Although preferred embodiments of the present invention have been described, it is created once a person skilled in the art knows basic
Property concept, then additional changes and modifications may be made to these embodiments.So it includes excellent that the following claims are intended to be interpreted as
It selects embodiment and falls into all change and modification of the scope of the invention.
Obviously, those skilled in the art can carry out various modification and variations without departing from this hair to the embodiment of the present invention
The spirit and scope of bright embodiment.In this way, if these modifications and variations of the embodiment of the present invention belong to the claims in the present invention
And its within the scope of equivalent technologies, then the present invention is also intended to include these modifications and variations.
Claims (12)
1. a kind of magnetic bead cleaning method, which is characterized in that including：
The cleaning solution of the first specified amount is injected into reaction cup to be cleaned；
The cleaning solution of the second specified amount is injected into the reaction cup to be cleaned, wherein injecting the clear of second specified amount
After washing lotion, cleaning solution liquid level is not less than the extreme higher position of the reaction cup wall of cup splashing liquid to be cleaned in the reaction cup to be cleaned；
Circulation executes n times or less and operates：Waste liquid in the reaction cup to be cleaned is drawn using the aspirating needle of the double-deck cleaning needle, and
The cleaning solution of third specified amount is injected into the reaction cup to be cleaned using the injection needle of the double-deck cleaning needle, wherein institute
The fluid injection needle guard of the double-deck cleaning needle is stated on the outside of aspirating needle, and the liquid injection port of the injection needle is higher than the liquid sucting port of aspirating needle, N is
Positive integer more than or equal to 1；
Waste liquid in the reaction cup to be cleaned is drawn, magnetic bead cleaning is completed.
2. the method as described in claim 1, which is characterized in that inject the cleaning of the first specified amount into reaction cup to be cleaned
Liquid, including：
When the pick-and-place station for detecting cleaning station has reaction cup to be cleaned, the reaction cup to be cleaned is rotated to the cleaning
The fluid injection station stood；And
The cleaning solution of the first specified amount is injected into the reaction cup to be cleaned using injection needle.
3. the method as described in claim 1, which is characterized in that drawn in the aspirating needle using the double-deck cleaning needle described to be cleaned
Before waste liquid in reaction cup, further comprise：Magnetic suck operation is carried out to the reaction cup to be cleaned；
After drawing the waste liquid in the reaction cup to be cleaned using the aspirating needle of the double-deck cleaning needle, further comprise：To institute
It states reaction cup to be cleaned and releases magnetic suck operation；
The injection needle using the double-deck cleaning needle injected into the reaction cup to be cleaned third specified amount cleaning solution it
Afterwards, further comprise：Mixing operation is carried out to the magnetic bead in the reaction cup to be cleaned.
4. the method according to claim 1, which is characterized in that the reaction cup to be cleaned is specified in injection third
After the cleaning solution of amount, it is high that cleaning solution liquid level is not less than the cleaning solution liquid level after the cleaning solution for injecting the second specified amount
Degree.
5. a kind of magnetic bead cleaning method, which is characterized in that including：
The cleaning solution of the first specified amount is injected into reaction cup to be cleaned；
Circulation executes M following operation：The second finger is injected into the reaction cup to be cleaned using the injection needle of the double-deck cleaning needle
Quantitative cleaning solution, wherein after the cleaning solution for injecting second specified amount, cleaning solution liquid level in the reaction cup to be cleaned
Not less than the extreme higher position of the reaction cup wall of cup splashing liquid to be cleaned, and using described in the aspirating needle absorption of the double-deck cleaning needle
Waste liquid in reaction cup to be cleaned, and the is injected into the reaction cup to be cleaned using the injection needle of the double-deck cleaning needle
The cleaning solution of three specified amounts, wherein the fluid injection needle guard of the bilayer cleaning needle is on the outside of aspirating needle, and the fluid injection of the injection needle
Mouth is higher than the liquid sucting port of aspirating needle, and M is the positive integer more than or equal to 1；
Waste liquid in the reaction cup to be cleaned is drawn, magnetic bead cleaning is completed.
6. method as claimed in claim 5, which is characterized in that cleaning of the reaction cup to be cleaned in injection third specified amount
After liquid, cleaning solution liquid level is not higher than the cleaning solution liquid level after the cleaning solution for injecting the second specified amount.
7. a kind of magnetic bead cleaning device, which is characterized in that including：
First injection unit, for injecting the cleaning solution of the first specified amount into reaction cup to be cleaned；
Second injection unit, for injecting the cleaning solution of the second specified amount into the reaction cup to be cleaned, wherein in injection institute
After the cleaning solution for stating the second specified amount, cleaning solution liquid level is not less than the reaction cup wall of cup to be cleaned in the reaction cup to be cleaned
The extreme higher position of splashing liquid；
Execution unit is operated for recycling execution n times or less：The reaction to be cleaned is drawn using the aspirating needle of the double-deck cleaning needle
Waste liquid in cup, and the clear of third specified amount is injected into the reaction cup to be cleaned using the injection needle of the double-deck cleaning needle
Washing lotion, wherein the fluid injection needle guard of the bilayer cleaning needle is on the outside of aspirating needle, and the liquid injection port of the injection needle is higher than aspirating needle
Liquid sucting port, N is positive integer more than or equal to 1；
Suction unit completes magnetic bead cleaning for drawing waste liquid in the reaction cup to be cleaned.
8. device as claimed in claim 7, which is characterized in that in the cleaning for injecting the first specified amount into reaction cup to be cleaned
When liquid, first injection unit is used for：
When the pick-and-place station for detecting cleaning station has reaction cup to be cleaned, the reaction cup to be cleaned is rotated to the cleaning
The fluid injection station stood；And
The cleaning solution of the first specified amount is injected into the reaction cup to be cleaned using injection needle.
9. device as claimed in claim 7, which is characterized in that drawn in the aspirating needle using the double-deck cleaning needle described to be cleaned
Before waste liquid in reaction cup, the execution unit is further used for：Magnetic suck operation is carried out to the reaction cup to be cleaned；
After drawing the waste liquid in the reaction cup to be cleaned using the aspirating needle of the double-deck cleaning needle, the execution unit is into one
Step is used for：Magnetic suck operation is released to the reaction cup to be cleaned；
The injection needle using the double-deck cleaning needle injected into the reaction cup to be cleaned third specified amount cleaning solution it
Afterwards, the execution unit is further used for：Mixing operation is carried out to the magnetic bead in the reaction cup to be cleaned.
10. such as the described in any item devices of claim 7-9, which is characterized in that the reaction cup to be cleaned refers in injection third
After quantitative cleaning solution, it is high that cleaning solution liquid level is not less than the cleaning solution liquid level after the cleaning solution for injecting the second specified amount
Degree.
11. a kind of magnetic bead cleaning device, which is characterized in that including：
Injection unit, for injecting the cleaning solution of the first specified amount into reaction cup to be cleaned；
Execution unit executes M following operation for recycling：Using the injection needle of the double-deck cleaning needle to the reaction cup to be cleaned
The cleaning solution of the second specified amount of middle injection, wherein after the cleaning solution for injecting second specified amount, the reaction cup to be cleaned
Middle cleaning solution liquid level is not less than the extreme higher position of the reaction cup wall of cup splashing liquid to be cleaned, and using the suction of the double-deck cleaning needle
Liquid needle draws the waste liquid in the reaction cup to be cleaned, and using the double-deck cleaning needle injection needle to described to be cleaned anti-
Answer the cleaning solution that third specified amount is injected in cup, wherein the fluid injection needle guard of the bilayer cleaning needle is and described on the outside of aspirating needle
The liquid injection port of injection needle is higher than the liquid sucting port of aspirating needle, and M is the positive integer more than or equal to 1；
Suction unit completes magnetic bead cleaning for drawing waste liquid in the reaction cup to be cleaned.
12. device as claimed in claim 11, which is characterized in that the reaction cup to be cleaned is clear injection third specified amount
After washing lotion, cleaning solution liquid level is not higher than the cleaning solution liquid level after the cleaning solution for injecting the second specified amount.
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| CN201810225779.5A CN108856112B (en) | 2018-03-19 | 2018-03-19 | Magnetic bead cleaning method and device |
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| CN201810225779.5A CN108856112B (en) | 2018-03-19 | 2018-03-19 | Magnetic bead cleaning method and device |
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| CN114669565A (en) * | 2022-03-25 | 2022-06-28 | 杭州奥盛仪器有限公司 | Waste liquid treatment system and treatment method |
| CN115156215A (en) * | 2022-08-02 | 2022-10-11 | 宋世琦 | Magnetic particle cleaning device and cleaning method for immunoassay device |
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