CN108855264A - A kind of multipurpose multi objective micro-fluidic chip - Google Patents
A kind of multipurpose multi objective micro-fluidic chip Download PDFInfo
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- CN108855264A CN108855264A CN201810763311.1A CN201810763311A CN108855264A CN 108855264 A CN108855264 A CN 108855264A CN 201810763311 A CN201810763311 A CN 201810763311A CN 108855264 A CN108855264 A CN 108855264A
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- fluidic chip
- multi objective
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- application zone
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/12—Specific details about manufacturing devices
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- Health & Medical Sciences (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
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- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physical Or Chemical Processes And Apparatus (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The present invention relates to micro-fluidic fields, disclose a kind of multipurpose multi objective micro-fluidic chip, and in particular to a kind of sample process, distribution, reaction, detects the multipurpose multi objective micro-fluidic chip being integrated, the multipurpose multi objective micro-fluidic chip includes lower plate, sealing is matched with the upper plate of the lower plate, cooperatively forms flow channel for liquids, reaction detection area by lower plate and upper plate, gas flow, sample application zone, microfluidic control valve, the structure functions area such as pump housing bonding pad.
Description
Technical field
The present invention relates to micro-fluidic field, more particularly to a kind of sample process, distribute, reaction, detect be integrated it is more
Purposes multi objective micro-fluidic chip.
Background technique
Micro-fluidic chip is a kind of current hot technology in analysis field, it be using micro-fluidic chip as carrier, with
The subjects such as chemistry, biology be combined with each other, and the whole process of analysis detection is realized on platform, have sample dosage few, reaction
Time is short, automation and the features such as integration degree is high.In recent years, with the high speed development of technical level, micro-fluidic chip is
More and more important role is played in biochemical, immune, analysis of molecules diagnostic field.
In order to realize quick multi objective parallel detection, need in micro-fluidic chip comprising multiple groups conversion zone and corresponding
Operation runner.In the method for operation of runner, micro-fluidic chip generally can be by external force such as electromagnetic force, centrifugal force, in phase
Close the operation control that fluid is realized under external force.Patent document 1 (101609088 A of CN) discloses a kind of base external electrical
The micro-fluidic chip of the driving of field force.In the method, mechanism and the equipment for generating electric field are complex；And in a manner of drop
Realize that the mode processing speed of fluid operation is slower.
Patent document 2 (103055973 A of CN) discloses a kind of micro-fluidic chip based on electroosmotic pump driving.This method
In, sample to be driven must have charge, and application range is limited.
Patent document 3 (102369443 A of CN) discloses a kind of micro-fluidic chip based on centrifugation power drive.In the party
In method, need specific mating centrifuge to provide driving force, and have certain requirement to centrifugal force, cause equipment overall structure and
Control system is complex.
Patent document 4 (CN 105548532A) discloses a kind of micro-fluidic chip based on air pump driving.This method application
In bioelectrode detection method, can not be applied to the detection method for having insulation request between multi objective.
Therefore, the technical difficult point faced at present be to research and develop it is a have a wide range of application, structure is simple, manufacture is convenient, makes
With convenient micro-fluidic chip, the driving of liquid is completed by easy method and fills each conversion zone completing reaction, thus
Realize quick multi objective parallel detection.
Summary of the invention
The invention mainly solves the technical problem of providing a kind of multipurpose multi objective micro-fluidic chip, the present invention passes through negative
Pressure mode drives fluid to run by predetermined manner.
In order to solve the above technical problems, one technical scheme adopted by the invention is that：It is micro- to provide a kind of multipurpose multi objective
Fluidic chip, including：Lower plate, sealing are matched with the upper plate of the lower plate, cooperatively form flow channel for liquids, anti-by lower plate and upper plate
Answer the structure functions areas such as detection zone, gas flow, sample application zone, microfluidic control valve, pump housing bonding pad.
Multipurpose multi objective micro-fluidic chip of the present invention, the flow channel for liquids front end connection sample application zone, and with
Reaction detection area connection.
Multipurpose multi objective micro-fluidic chip of the present invention, the flow channel for liquids can be one be also possible to it is more
Item.
Multipurpose multi objective micro-fluidic chip of the present invention, the gas flow and reaction detection area connection, end
End connection pump housing bonding pad.
Multipurpose multi objective micro-fluidic chip of the present invention, the sample application zone can be a cavity, can also be with
Additional filter membrane.
Multipurpose multi objective micro-fluidic chip of the present invention, the microfluidic control valve can be by lower plate and upper plate
Structure cooperatively forms, can also be poly- with additional low density polyethylene (LDPE) (LDPE), linear low density polyethylene (LLDPE), extra-low density
Ethylene (VLDPE), medium density polyethylene (MEPE), high density polyethylene (HDPE) (HDPE), polyvinyl chloride (PVC), polytetrafluoroethylene (PTFE)
(PTFE), polystyrene (PS), polyvinyl alcohol (PVA), polypropylene (PP) and polyethylene terephthalate (PET) etc. are ventilative
Blocking solution accessory.
Sample application zone of the present invention, the filter membrane can be single layer, be also possible to multiple-layer stacked.
Sample application zone of the present invention, the filter membrane can select polysulfones (PS), polyarylsulfone (PAS) (PASF), Poly-s 179
(PES), the substances such as polyethylene (PE) and polycarbonate (PC).
Multipurpose multi objective micro-fluidic chip of the present invention, can drive liquid flowing by air pump, and air pump provides
Negative pressure effectively avoids sample from contacting with the external world, pollutes.
Multipurpose multi objective micro-fluidic chip of the present invention can pass through transmission, scattering, reflection, fluorescence, luminous etc.
Detection means carries out instrument or visually observe and is measured in real time or end point determination.
Detailed description of the invention
It is better understood with the present invention according to following non-limiting figure, wherein：
Fig. 1 show the multipurpose multi objective microfluidic chip structure schematic diagram of the offer of the embodiment of the present invention 1；
Fig. 2 show the multipurpose multi objective microfluidic chip structure schematic diagram of the offer of the embodiment of the present invention 2；
Fig. 3 show the multipurpose multi objective micro-fluidic chip top view of the offer of the embodiment of the present invention 2；
The multipurpose multi objective micro-fluidic chip different cross section that Fig. 4 (a)~(b) show the offer of the embodiment of the present invention 2 cuts open
Face figure；
Fig. 5 (a)~(b) show the multipurpose multi objective micro-fluidic chip difference well of the offer of the embodiment of the present invention 2
The sectional view of structure；
Specific embodiment
With reference to the attached drawing in the embodiment of the present invention, technical solution in the embodiment of the present invention is purged, completely
Description, it is clear that the described embodiment is only a part of the embodiment of the present invention, instead of all the embodiments., it is based on this hair
Embodiment in bright, every other implementation obtained by those of ordinary skill in the art without making creative efforts
Example, shall fall within the protection scope of the present invention.
Embodiment 1：
Fig. 1 is the structure chart for indicating the embodiment 1 of multipurpose multi objective micro-fluidic chip of the present invention.Multipurpose of the invention
Multi objective micro-fluidic chip has：Lower plate 101, sealing are matched with the upper plate 102 of the lower plate 101, pass through lower plate 101 and upper plate
102 cooperatively form flow channel for liquids 103, multiple reaction detection areas 104, gas flow 105, sample application zone 106, microfluidic control valve
107, pump housing bonding pad 108.
Multipurpose multi objective micro-fluidic chip of the invention is provided by the air pump connecting with pump housing bonding pad 108
Negative pressure, external air pump sequence is in pump housing bonding pad 108, gas flow 105, microfluidic control valve 107, reaction detection area 104, liquid
Body runner 103, sample application zone 106 form negative pressure, and negative pressure drives liquid sample by sample application zone 106,103 flow distribution of flow channel for liquids
To each reaction detection area 104, microfluidic control valve 107 carries out the barrier between liquids and gases runner 105, to realize phase
Close the multipurpose multi objective micro-fluidic chip of detection.
Multipurpose multi objective micro-fluidic chip of the invention, lower plate 101 and upper plate 102 can pass through contact adhesive, light
Sensitive adhesive, heat sensitive adhesive, double-sided adhesive, thermal bonding, ultrasonic bond, protrusion are coupled to the methods such as groove and are engaged.
Multipurpose multi objective micro-fluidic chip of the invention, which is characterized in that 103 front end of flow channel for liquids and sample application zone 106
Connection, middle part are connected to multiple reaction detection areas 104.Primary flow path 103 extends in sinuous serpentine configuration realizing route, therefore can
Reaction detection area 104 is filled to provide time enough for fluid.
Sample application zone 106 passes through 106 influent runner 103 of sample application zone for the temporary of fluid sample, fluid sample.
As described above, the negative pressure provided by pump housing bonding pad 108, liquid are first flowed into from sample application zone 106a to flow channel for liquids
103a is then filled each reaction detection area 104a；Liquid is first flowed into from sample application zone 106b to flow channel for liquids 103b, and
Each reaction detection area 104b is filled afterwards.
As shown in Figure 1, the sample of different number can be examined simultaneously in a multipurpose multi objective micro-fluidic chip.
Different samples can be identical source and be also possible to separate sources, can be same type and is also possible to different type.
For example, the urine specimen of a patient can be added in sample application zone 106a, another trouble can be added in sample application zone 106b
Plasma sample.
As shown in Figure 1, sample application zone 106 is rectangular, but it can be other arbitrary shapes, the volume of sample application zone 106 determines
The volume of sample to be tested.User can be used pipette, inhale the transfer that the liquid shifting equipments devices such as dropper carry out sample.
The acceptable sample in sample application zone 106 includes but is not limited to various fluid samples, such as blood, blood plasma, serum, tissue
The body fluid such as liquid, lymph, cerebrospinal fluid, sperm, saliva, urine or environmental water sample or various liquid extracts.
As shown in Figure 1, microfluidic control valve 107 is cooperatively formed by lower plate 101 and upper plate 102, the microfluidic control valve 107
Depth preferably from about 0.005mm to about 0.1mm, preferred depth are about 0.01mm to about 0.05mm, can be realized reaction detection
Being isolated between area 104 and gas flow 105.
The multipurpose multi objective micro-fluidic chip can be by being capable of integrated injection molding and on chemistry and biology
Non-active material is formed.For example, the multipurpose multi objective micro-fluidic chip can be formed by a variety of materials, which includes
But be not limited to plastics, glass, mica, silica and semiconductor wafer, the plastics include polymethyl methacrylate (PMMA),
Dimethione (PDMS), polycarbonate (PC), low density polyethylene (LDPE) (LDPE), linear low density polyethylene (LLDPE), pole
Low density polyethylene (LDPE) (VLDPE), medium density polyethylene (MEPE), high density polyethylene (HDPE) (HDPE), polyvinyl chloride (PVC), poly- four
Vinyl fluoride (PTFE), polystyrene (PS), polyvinyl alcohol (PVA), polypropylene (PP) and polyethylene terephthalate
(PET), propylene cyanogen-butadiene-styrene (ABS), cyclenes copolymer (COC) etc..Therefore, any and material may be used to this
Multipurpose multi objective micro-fluidic chip only requires that the machinery of its nonactive and stability with chemistry and biology is processable.
Embodiment 2：
Fig. 2~5 are the structure charts for indicating the embodiment 2 of multipurpose multi objective micro-fluidic chip of the present invention.Of the invention is more
Purposes multi objective micro-fluidic chip has：Lower plate 201, sealing are matched with the upper plate 202 of the lower plate 201, by lower plate 201 with
Upper plate 202 cooperatively forms flow channel for liquids 203, multiple reaction detection areas 204, gas flow 205, sample application zone 206, filter element
207, microfluidic control valve 208, pump housing bonding pad 209.
Multipurpose multi objective micro-fluidic chip of the invention is provided by the air pump connecting with pump housing bonding pad 209
Negative pressure, external air pump sequence is in pump housing bonding pad 209, gas flow 205, microfluidic control valve 208, reaction detection area 204, liquid
Body runner 203, sample application zone 206 form negative pressure, and negative pressure drives liquid sample by sample application zone 206,203 flow distribution of flow channel for liquids
To each reaction detection area 204, microfluidic control valve 208 carries out the barrier between liquids and gases runner 205, to realize phase
Close the multipurpose multi objective micro-fluidic chip of detection.
Multipurpose multi objective micro-fluidic chip of the invention, lower plate 201 and upper plate 202 can pass through contact adhesive, light
Sensitive adhesive, heat sensitive adhesive, double-sided adhesive, thermal bonding, ultrasonic bond, protrusion are coupled to the methods such as groove and are engaged.
Multipurpose multi objective micro-fluidic chip of the invention, which is characterized in that 203 front end of flow channel for liquids and sample application zone 206
Connection, middle part are connected to multiple reaction detection areas 204.
As shown in Fig. 2, sample application zone 106 is circle, but it can be other arbitrary shapes, the volume of sample application zone 106 determines
The volume of sample to be tested.User can be used pipette, inhale the transfer that the liquid shifting equipments devices such as dropper carry out sample.
The acceptable sample in sample application zone 106 includes but is not limited to various fluid samples, such as blood, blood plasma, serum, tissue
The body fluid such as liquid, lymph, cerebrospinal fluid, sperm, saliva, urine or environmental water sample or various liquid extracts.
Sample application zone 206 passes through 206 influent runner 203 of sample application zone for the temporary of fluid sample, fluid sample.
As described above, the negative pressure provided by pump housing bonding pad 209, liquid are first flowed into from sample application zone 206 to flow channel for liquids
203, then each reaction detection area 204 is filled.
As shown in Fig. 4 (a), microfluidic control valve 208 is a kind of blocking solution gas permeable material, as low density polyethylene (LDPE) (LDPE),
Linear low density polyethylene (LLDPE), very low density polyethylene (VLDPE), medium density polyethylene (MEPE), high density polyethylene (HDPE)
(HDPE), polyvinyl chloride (PVC), polytetrafluoroethylene (PTFE) (PTFE), polystyrene (PS), polyvinyl alcohol (PVA), polypropylene (PP) and
Polyethylene terephthalate (PET) etc., can be realized being isolated between reaction detection area 204 and gas flow 205.
The multipurpose multi objective micro-fluidic chip can be by being capable of integrated injection molding and on chemistry and biology
Non-active material is formed.For example, the multipurpose multi objective micro-fluidic chip can be formed by a variety of materials, which includes
But be not limited to plastics, glass, mica, silica and semiconductor wafer, the plastics include polymethyl methacrylate (PMMA),
Dimethione (PDMS), polycarbonate (PC), low density polyethylene (LDPE) (LDPE), linear low density polyethylene (LLDPE), pole
Low density polyethylene (LDPE) (VLDPE), medium density polyethylene (MEPE), high density polyethylene (HDPE) (HDPE), polyvinyl chloride (PVC), poly- four
Vinyl fluoride (PTFE), polystyrene (PS), polyvinyl alcohol (PVA), polypropylene (PP) and polyethylene terephthalate
(PET), propylene cyanogen-butadiene-styrene (ABS), cyclenes copolymer (COC) etc..Therefore, any and material may be used to this
Multipurpose multi objective micro-fluidic chip only requires that the machinery of its nonactive and stability with chemistry and biology is processable.
As shown in Fig. 5 (a)~(b), filter element 207 may include polysulfones (PS), gather for filtering sample, filter element
Fragrant sulfone (PASF), Poly-s 179 (PES), polyethylene (PE) and polycarbonate (PC) etc. have the substance of porous structure.
The filter membrane can be used directly, be used after can also being handled, and the antibody of predetermined substance is such as coated with.
For example, when sample is blood, it is thin that the blood sample accommodated in sample application zone 106 by filter element 207 filters out blood
Born of the same parents, the blood plasma or serum influent runner 103 of acquisition.
Referring to Fig. 5 (a), filter element 207 can only include one layer of filter membrane 207, as described above, filter membrane is for filtering
Sample.
Referring to Fig. 5 (b), filter element 207 may include double-deck filter membrane 207a and 207b, as described above, filter membrane
For filtering sample, pass through filter membrane 207b, the inert matter in sample again after filter membrane 207a in filter element 207
By further progress filtration treatment.
Fig. 5 (a) and Fig. 5 (b) only illustrates the example of the filter element 207 containing different number filter membranes.Therefore, it takes
The substance to be filtered certainly in sample, filter membrane can contain three layers or more layers.
Claims (8)
1. a kind of multipurpose multi objective micro-fluidic chip, which is characterized in that including：Lower plate, sealing are matched with the upper of the lower plate
Plate cooperatively forms flow channel for liquids, reaction detection area, gas flow, sample application zone, microfluidic control valve, pump by lower plate and upper plate
The structure functions area such as body bonding pad.
2. multipurpose multi objective micro-fluidic chip according to claim 1, it is characterised in that：The flow channel for liquids front end connects
Connect sample application zone, and with reaction detection area connection.
3. multipurpose multi objective micro-fluidic chip according to claim 1, it is characterised in that：The flow channel for liquids can be
One be also possible to it is a plurality of.
4. multipurpose multi objective micro-fluidic chip according to claim 1, it is characterised in that：The gas flow with react
Detection zone connection, end connect pump housing bonding pad.
5. multipurpose multi objective micro-fluidic chip according to claim 1, it is characterised in that：The sample application zone can be one
A cavity can also add filter membrane.
6. multipurpose multi objective micro-fluidic chip according to claim 1, it is characterised in that：The microfluidic control valve can
It, can also be with additional low density polyethylene (LDPE) (LDPE), linear low density polyethylene to be cooperatively formed by lower plate and upper plate structure
(LLDPE), very low density polyethylene (VLDPE), medium density polyethylene (MEPE), high density polyethylene (HDPE) (HDPE), polyvinyl chloride
(PVC), polytetrafluoroethylene (PTFE) (PTFE), polystyrene (PS), polyvinyl alcohol (PVA), polypropylene (PP) and poly terephthalic acid second
The ventilative blocking solution accessory such as diol ester (PET).
7. sample application zone according to claim 5, it is characterised in that：The filter membrane can be single layer, be also possible to multilayer
Superposition.
8. sample application zone according to claim 5, it is characterised in that：The filter membrane can select polysulfones (PS), polyarylsulfone (PAS)
(PASF), the substances such as Poly-s 179 (PES), polyethylene (PE) and polycarbonate (PC).
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810763311.1A CN108855264A (en) | 2018-07-12 | 2018-07-12 | A kind of multipurpose multi objective micro-fluidic chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810763311.1A CN108855264A (en) | 2018-07-12 | 2018-07-12 | A kind of multipurpose multi objective micro-fluidic chip |
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| CN108855264A true CN108855264A (en) | 2018-11-23 |
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Application publication date: 20181123 |