CN108849526A - The tissue cultivating method of glaucocalyx rabdosia herb - Google Patents
The tissue cultivating method of glaucocalyx rabdosia herb Download PDFInfo
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- CN108849526A CN108849526A CN201810945010.0A CN201810945010A CN108849526A CN 108849526 A CN108849526 A CN 108849526A CN 201810945010 A CN201810945010 A CN 201810945010A CN 108849526 A CN108849526 A CN 108849526A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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Abstract
The invention discloses a kind of tissue cultivating methods of glaucocalyx rabdosia herb, include the following steps：(1) explant is handled, and (2) callus tissue culture, (3) lure bud culture, (4) Multiplying culture,（5）Culture of rootage,（6）Hardening.The tissue cultivating method of glaucocalyx rabdosia herb of the present invention, cultivation period is short, obtained glaucocalyx rabdosia herb seedling is best in quality, high survival rate after transplanting, up to 92% or more, be transplanted to adapt to after natural environment environment ability it is strong, pest and disease damage is few, it helps to ensure that the yield of glaucocalyx rabdosia herb, while providing reference to be further improved the tissue cultivating technology of glaucocalyx rabdosia herb.
Description
Technical field
The present invention relates to Chinese herbal medicine cultivation technology fields, and in particular to the tissue cultivating method of glaucocalyx rabdosia herb.
Background technique
Glaucocalyx rabdosia herb, Classification system：Rabdosia tenuifolia (D. Don) Hara, also known as：Spire scented tea
Dish, Folium solani verbascifolii, typhoid fever head, meadow fescue, ternateleaf rabdosia herb etc. are Labiatae Rabdosia plant.Undershrub, up to 1 centimetre, extremely more points
Branch.Old branch are closely cylindrical, have corner angle, and khaki, cortex longitudinally peels off, sprout elongation, and weak, blunt four prismatic is extremely close grey
White flock hair.All herbal medicine, mildly bitter flavor is cool in nature, has the function of clearing heat and detoxicating, removing jaundice dampness removing, detumescence, preventing phlegm from forming and stopping coughing, controls
Dysentery enteritis, icterepatitis, smoke uptake is scorching, tonsillitis, urinary tract infection, cystitis, acute nephritis, swelling pain, influenza, poison
Snake bite, toothache etc..Glaucocalyx rabdosia herb is grown on the hillside of height above sea level 140-2200m, in cheuch thick grass.China is distributed mainly on
Guangxi, Guangdong, Guizhou, Yunnan etc., wherein Guangxi is most, is mainly distributed on the ground such as Guangxi Hechi, Baise, Nanning, Yulin, Wuzhou
Area is one of Guangxi Special Traditional Chinese Medicine material.
Strong doctor's recipe " Folium solani verbascifolii soup " is exactly to use Folium solani verbascifolii（That is glaucocalyx rabdosia herb）With made of screwtree root, Folium solani verbascifolii soup is
One of clearing heat and detoxicating side of Zhuang.In addition, glaucocalyx rabdosia herb has apparent curative effect to treatment hepatitis B, it is compound glaucocalyx rabdosia herb
Monarch drug in a prescription in piece.Studies have shown that the important activity ingredient that glaucocalyx rabdosia herb contains protection liver and promotes glycogen primary.The ingredient
By killing the virus in liver cell, reaches purification liver environment, repair liver cell, the final hepatitis virus killed in blood.
The research achievement is incorporated the mysterious of century-old precious jade medicine and is formulated by Guangxi Gold show shrine medicine company Co., Ltd, develops finished product precious jade medicine
Compound glaucocalyx rabdosia herb piece, the product have significant curative effect according to clinical verification, to chronic hepatitis B and hepatitis carrier, make HBV
Loss of activity is fairly obvious, and State Administration of Traditional Chinese Medicine's comparison gives high evaluation, has solemnly issued assay certificate in Beijing.
With the successful exploitation of the Chinese patent drugs products such as compound glaucocalyx rabdosia herb piece, increase to the demand of medicinal material, wild money
Source has been unable to meet the market demand.Also have in open source literature at present on a small quantity about the artificial cultivation technique of Rabdosia amethystoides and nursery skill
Art, but still supply falls short of demand for the Rabdosia amethystoides planted.Due to shortage of resources, purchase volume declines year by year, and part pharmacy corporation is forced to stop
It only produces, carrying out artificial cultivation has been the task of top priority, and the primary premise for carrying out a large amount of artificial cultivations is exactly the offer of seedling.Cause
This, it is extremely important can to cultivate large batch of high-quality glaucocalyx rabdosia herb seedling.
From 2004, Guangxi carried out the research of the wild change man kind of glaucocalyx rabdosia herb and has obtained initial success.Three leaves are fragrant
The cultivation of tea dish medicinal material is mainly bred with seed, however the adaptability and vitality of glaucocalyx rabdosia herb seed are not strong enough, by
In lacking seed testing standard and effective management, the event that inferior strain is cheated the farmers in seed transaction happens occasionally, and gives you agriculture
The people cause biggish economic loss, and the people for also resulting in plantation is fewer and fewer.And the period of cuttage and seedling culture is longer, peasant is due to behaviour
Make lack of standardization to be also difficult to obtain higher survival rate.
Summary of the invention
To solve the above-mentioned problems, the glaucocalyx rabdosia herb of high-quality health is provided for the high-volume artificial growth of glaucocalyx rabdosia herb
Seedling, the present invention provides the tissue cultivating method of glaucocalyx rabdosia herb, this method can significantly improve glaucocalyx rabdosia herb seedling at
Motility rate guarantees the yield of glaucocalyx rabdosia herb after transplanting.
The invention is realized by the following technical scheme：
The tissue cultivating method of glaucocalyx rabdosia herb, includes the following steps：
（1）Explant processing：In glaucocalyx rabdosia herb rudiment in 3~April, the complete axillary bud of glaucocalyx rabdosia herb of no disease and pests harm is acquired
As explant, 1~2min is rinsed with pure water, 10 are impregnated with the liquor potassic permanganate that mass fraction is 0.25~0.35%~
20min, then with alcohol disinfecting 20~30 seconds of 70~75%, then use aseptic water washing 2~3 times, natural air drying is spare；
（2）Callus tissue culture：In an aseptic environment, axillary bud is put into callus tissue culture base and is cultivated, in temperature 25 ± 1
DEG C, dark culture 3~5 days, then carry out optical culture 10~15 days, 24~28 DEG C of optical culture temperature, intensity of illumination 3000~
3300lux obtains callus；
The callus tissue culture based formulas is：MS+0.22~0.25mg/L+0.45~0.52mg/L of 6-BA NAA+
Sucrose+4g/L the agar of 3.0g/L, pH5.8~6.0.
（3）Lure bud culture：In an aseptic environment, continue culture 12~16 days in callus tissue culture base, cultivation temperature
25~28 DEG C, 3200~3400lux of intensity of illumination, axillary buds formation is more and starts to grow young leaflet tablet, can terminate；
（4）Multiplying culture：It is transferred to subculture multiplication medium to continue to cultivate, culture environment：25~28 DEG C of temperature, intensity of illumination
3200~3500lux, light application time 12~14h/ days, incubation time 15~20 days, the leaf color to no offspring started to change
When can terminate；
The subculture multiplication medium formula is：MS+0.22~0.25mg/L+0.48~0.53mg/L of 6-BA IBA+
Sucrose+5g/L the agar of 3.0g/L, pH5.8~6.0.
（5）Culture of rootage：It goes to and carries out culture of rootage, condition of culture on root media：25 ± 2 DEG C of temperature, illumination is strong
Spend 2800~3100lux；Culture 27~35 days, the root long to most of seedling reaches 3~5cm, and radical >=5 can terminate；
The prescription of rooting medium is：1/2MS+0.45~0.52mg/L NAA+0.48~0.53mg/L IBA+3.5g/
Sucrose+5.5g/L the agar of L, pH5.8~6.0.
（6）Hardening：Using greenhouse seedbed hardening, medium of seedling bed sandy soil in mass ratio：Vermiculite：Humic acid is 10:9:
1, transplanting to seedbed is placed on shady place, and pours appropriate root water, is transferred at sunlight after Miao Dinggen, continues under natural conditions
Culture 25~30 days, hardening are completed.
Beneficial effects of the present invention：
1, the tissue cultivating method of glaucocalyx rabdosia herb of the present invention, cultivation period is short, obtained glaucocalyx rabdosia herb seedling quality
Excellent, high survival rate, be transplanted to adapt to after natural environment environment ability it is strong, pest and disease damage is few, helps to ensure that glaucocalyx rabdosia herb
Yield, while for be further improved glaucocalyx rabdosia herb tissue cultivating technology reference is provided.
2, the tissue cultivating method of glaucocalyx rabdosia herb of the present invention, by reasonable disposition culture medium, stringent optimization is simultaneously
Cultivation condition is controlled, callus formation rate up to 80% or more, transplant by 87% or more sprout-induction rate, 92% or more root induction rate
Survival rate is up to 92% afterwards.
3, the tissue cultivating method of present invention research glaucocalyx rabdosia herb can be carried out by special technical staff or mechanism
High-volume is cultivated, and is provided enough seedling sources for high-volume cultivation glaucocalyx rabdosia herb, can be sold to peasant and carry out transplanting plantation, have
Conducive to avoiding peasant from abandoning planting, causing the status of scarcity of resources since the low planting cost of survival rate is big.
Specific embodiment
In order to keep technical solution of the present invention and advantage clearer, below with reference to the embodiment of the present invention, to the present invention
Technical solution be clearly and completely described.
Embodiment 1
The tissue cultivating method of glaucocalyx rabdosia herb, includes the following steps：
（1）Explant processing：In glaucocalyx rabdosia herb rudiment in March, the complete axillary bud of glaucocalyx rabdosia herb for acquiring no disease and pests harm is made
For explant, 2min is rinsed with pure water, impregnates 15min with the liquor potassic permanganate that mass fraction is 0.30%, then with 70%
It alcohol disinfecting 20 seconds, then uses aseptic water washing 2 times, natural air drying is spare；
（2）Callus tissue culture：In an aseptic environment, axillary bud is put into callus tissue culture base and is cultivated, in temperature（25±1）
DEG C, dark culture 3 days, then carry out optical culture 12 days, 24~28 DEG C of optical culture temperature, 3000~3300lux of intensity of illumination is cured
Injured tissue；
The callus tissue culture based formulas is：The sucrose of MS+0.24mg/L 6-BA+0.48mg/L NAA+3.0g/L+
4g/L agar, pH5.8.
（3）Lure bud culture：In an aseptic environment, continue to cultivate 15 days in callus tissue culture base, cultivation temperature 25~
28 DEG C, 3200~3400lux of intensity of illumination, axillary buds formation is more and starts to grow young leaflet tablet, can terminate；
（4）Multiplying culture：It is transferred to subculture multiplication medium to continue to cultivate, culture environment：25~28 DEG C of temperature, intensity of illumination
3200~3500lux, light application time 12~14h/ days, incubation time 18 days, when the leaf color of no offspring starts variation i.e.
It can terminate；
The subculture multiplication medium formula is：The sucrose of MS+0.22mg/L 6-BA+0.50mg/L IBA+3.0g/L+
5g/L agar, pH5.8.
（5）Culture of rootage：It goes to and carries out culture of rootage, condition of culture on root media：Temperature（25±2）DEG C, illumination
2800~3100lux of intensity；Culture 31 days, the root long to most of seedling reaches 3~5cm, and radical >=5 can terminate；
The prescription of rooting medium is：The sucrose of 1/2MS+0.48mg/L NAA+0.48mg/L IBA+3.5g/L+
5.5g/L agar, pH5.9.
（6）Hardening：Using greenhouse seedbed hardening, medium of seedling bed sandy soil in mass ratio：Vermiculite：Humic acid is 10:9:
1, transplanting to seedbed is placed on shady place, and pours appropriate root water, is transferred at sunlight after Miao Dinggen, continues under natural conditions
Culture 28 days, hardening are completed.
Embodiment 2
The tissue cultivating method of glaucocalyx rabdosia herb, includes the following steps：
（1）Explant processing：In glaucocalyx rabdosia herb rudiment in March, the complete axillary bud of glaucocalyx rabdosia herb for acquiring no disease and pests harm is made
For explant, 2min is rinsed with pure water, impregnates 15min with the liquor potassic permanganate that mass fraction is 0.30%, then with 70%
It alcohol disinfecting 20 seconds, then uses aseptic water washing 2 times, natural air drying is spare；
（2）Callus tissue culture：In an aseptic environment, axillary bud is put into callus tissue culture base and is cultivated, in temperature（25±1）
DEG C, dark culture 3 days, then carry out optical culture 12 days, 24~28 DEG C of optical culture temperature, 3000~3300lux of intensity of illumination is cured
Injured tissue；
The callus tissue culture based formulas is：The sucrose of MS+0.22mg/L 6-BA+0.46mg/L NAA+3.0g/L+
4g/L agar, pH5.8.
（3）Lure bud culture：In an aseptic environment, continue to cultivate 14 days in callus tissue culture base, cultivation temperature 25~
28 DEG C, 3200~3400lux of intensity of illumination, axillary buds formation is more and starts to grow young leaflet tablet, can terminate；
（4）Multiplying culture：It is transferred to subculture multiplication medium to continue to cultivate, culture environment：25~28 DEG C of temperature, intensity of illumination
3200~3500lux, light application time 12~14h/ days, incubation time 18 days, when the leaf color of no offspring starts variation i.e.
It can terminate；
The subculture multiplication medium formula is：The sucrose of MS+0.22mg/L 6-BA+0.51mg/L IBA+3.0g/L+
5g/L agar, pH5.8.
（5）Culture of rootage：It goes to and carries out culture of rootage, condition of culture on root media：Temperature（25±2）DEG C, illumination
2800~3100lux of intensity；Culture 33 days, the root long to most of seedling reaches 3~5cm, and radical >=5 can terminate；
The prescription of rooting medium is：The sucrose of 1/2MS+0.46mg/L NAA+0.52mg/L IBA+3.5g/L+
5.5g/L agar, pH6.0.
（6）Hardening：Using greenhouse seedbed hardening, medium of seedling bed sandy soil in mass ratio：Vermiculite：Humic acid is 10:9:
1, transplanting to seedbed is placed on shady place, and pours appropriate root water, is transferred at sunlight after Miao Dinggen, continues under natural conditions
Culture 29 days, hardening are completed.
Embodiment 3
（1）Explant processing：In glaucocalyx rabdosia herb rudiment in March, the complete axillary bud of glaucocalyx rabdosia herb for acquiring no disease and pests harm is made
For explant, 2min is rinsed with pure water, impregnates 15min with the liquor potassic permanganate that mass fraction is 0.30%, then with 75%
It alcohol disinfecting 20 seconds, then uses aseptic water washing 2 times, natural air drying is spare；
（2）Callus tissue culture：In an aseptic environment, axillary bud is put into callus tissue culture base and is cultivated, in temperature（25±1）
DEG C, dark culture 3 days, then carry out optical culture 10 days, 24~26 DEG C of optical culture temperature, 3000~3300lux of intensity of illumination is cured
Injured tissue；
The callus tissue culture based formulas is：The sucrose of MS+0.23mg/L 6-BA+0.47mg/L NAA+3.0g/L+
4g/L agar, pH5.8.
（3）Lure bud culture：In an aseptic environment, continue to cultivate 15 days in callus tissue culture base, cultivation temperature 25~
28 DEG C, 3200~3400lux of intensity of illumination, axillary buds formation is more and starts to grow young leaflet tablet, can terminate；
（4）Multiplying culture：It is transferred to subculture multiplication medium to continue to cultivate, culture environment：25~28 DEG C of temperature, intensity of illumination
3200~3500lux, light application time 12~14h/ days, incubation time 18 days, when the leaf color of no offspring starts variation i.e.
It can terminate；
The subculture multiplication medium formula is：The sucrose of MS+0.24mg/L 6-BA+0.50mg/L IBA+3.0g/L+
5g/L agar, pH5.8.
（5）Culture of rootage：It goes to and carries out culture of rootage, condition of culture on root media：Temperature（25±2）DEG C, illumination
2800~3100lux of intensity；Culture 28 days, the root long to most of seedling reaches 3~5cm, and radical >=5 can terminate；
The prescription of rooting medium is：The sucrose of 1/2MS+0.48mg/L NAA+0.52mg/L IBA+3.5g/L+
5.5g/L agar, pH6.0.
（6）Hardening：Using greenhouse seedbed hardening, medium of seedling bed sandy soil in mass ratio：Vermiculite：Humic acid is 10:9:
1, transplanting to seedbed is placed on shady place, and pours appropriate root water, is transferred at sunlight after Miao Dinggen, continues under natural conditions
Culture 30 days, hardening are completed.
Glaucocalyx rabdosia herb obtained in the embodiment of the present invention and seed seedling-raising, the survival rate of cuttage and seedling culture and growing-seedling period
It is as follows compared to relatively：
| Survival rate（%） | Growing-seedling period（It） | Pest and disease damage situation | |
| Embodiment 1 | 92.5% | 107 | Substantially without |
| Embodiment 2 | 93.0% | 109 | Substantially without |
| Embodiment 3 | 93.0% | 104 | Substantially without |
| Seed seedling-raising | 60~70% | 60~80 | Pest and disease damage is more, needs frequent insect prevention deinsectization |
| Cuttage and seedling culture | 75~85% | 110~130 | Pest and disease damage is relatively fewer |
It is above-mentioned as it can be seen that the tissue cultivating method survival rate of glaucocalyx rabdosia herb of the present invention is educated much higher than seed seedling-raising and cuttage
Seedling is planted by subsequent transplanting and is counted, and the ability of disease and insect resistance significantly improves, and pest and disease damage is seldom, helps to ensure that three leaves
The yield of Rabdosia amethystoides；Although the seed seedling-raising period is shorter, the adaptability and vitality of glaucocalyx rabdosia herb seed are not strong enough, survive
Rate is very low, and its longer germination rate of time of seeds is lower, and the ability of the seedling disease and insect resistance survived is also much worse than other
Two methods；Cuttage and seedling culture this processing tissue culture and seed seedling-raising both methods before, survival rate is general, growing-seedling period compared with
It is long, and misoperation easily leads to high-volume death.
Claims (4)
1. the tissue cultivating method of glaucocalyx rabdosia herb, which is characterized in that include the following steps：
（1）Explant processing：In glaucocalyx rabdosia herb rudiment in 3~April, the complete axillary bud of glaucocalyx rabdosia herb of no disease and pests harm is acquired
As explant, 1~2min is rinsed with pure water, 10 are impregnated with the liquor potassic permanganate that mass fraction is 0.25~0.35%~
20min, then with alcohol disinfecting 20~30 seconds of 70~75%, then use aseptic water washing 2~3 times, natural air drying is spare；
（2）Callus tissue culture：In an aseptic environment, axillary bud is put into callus tissue culture base and is cultivated, in temperature 25 ± 1
DEG C, dark culture 3~5 days, then carry out optical culture 10~15 days, 24~28 DEG C of optical culture temperature, intensity of illumination 3000~
3300lux obtains callus；
（3）Lure bud culture：In an aseptic environment, continue in callus tissue culture base culture 12~16 days, cultivation temperature 25~
28 DEG C, 3200~3400lux of intensity of illumination, axillary buds formation is more and starts to grow young leaflet tablet, can terminate；
（4）Multiplying culture：It is transferred to subculture multiplication medium to continue to cultivate, culture environment：25~28 DEG C of temperature, intensity of illumination
3200~3500lux, light application time 12~14h/ days, incubation time 15~20 days, the leaf color to no offspring started to change
When can terminate；
（5）Culture of rootage：It goes to and carries out culture of rootage, condition of culture on root media：25 ± 2 DEG C of temperature, intensity of illumination
2800~3100lux；Culture 27~35 days, the root long to most of seedling reaches 3~5cm, and radical >=5 can terminate；
（6）Hardening：Using greenhouse seedbed hardening, medium of seedling bed sandy soil in mass ratio：Vermiculite：Humic acid is 10:9:1, it moves
It plants to seedbed and is placed on shady place, and pour appropriate root water, be transferred at sunlight after Miao Dinggen, continue to cultivate under natural conditions
25~30 days, hardening was completed.
2. the tissue cultivating method of glaucocalyx rabdosia herb according to claim 1, which is characterized in that the callus tissue culture base
Formula is：MS+0.22~0.25mg/L 6-BA+0.45~0.52mg/L NAA+3.0g/L sucrose+4g/L agar,
PH5.8~6.0.
3. the tissue cultivating method of glaucocalyx rabdosia herb according to claim 1, which is characterized in that the shoot proliferation culture
Based formulas is：MS+0.22~0.25mg/L 6-BA+0.48~0.53mg/L IBA+3.0g/L sucrose+5g/L agar,
PH5.8~6.0.
4. the tissue cultivating method of glaucocalyx rabdosia herb according to claim 1, which is characterized in that the prescription of rooting medium
For：1/2MS+0.45~0.52mg/L NAA+0.48~0.53mg/L IBA+3.5g/L sucrose+5.5g/L agar,
PH5.8~6.0.
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| CN201810945010.0A CN108849526A (en) | 2018-08-20 | 2018-08-20 | The tissue cultivating method of glaucocalyx rabdosia herb |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109362566A (en) * | 2018-11-27 | 2019-02-22 | 钟天路 | A kind of Rabdosia amethystoides tissue culture and rapid propagation method |
| CN110972829A (en) * | 2019-12-19 | 2020-04-10 | 美尚生态景观股份有限公司 | Method for transplanting and hardening tissue culture seedlings of rabdosia nervosa |
-
2018
- 2018-08-20 CN CN201810945010.0A patent/CN108849526A/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| 闫志刚等: ""三叶香茶菜的组织培养及植株再生"", 《植物生理学通讯》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109362566A (en) * | 2018-11-27 | 2019-02-22 | 钟天路 | A kind of Rabdosia amethystoides tissue culture and rapid propagation method |
| CN110972829A (en) * | 2019-12-19 | 2020-04-10 | 美尚生态景观股份有限公司 | Method for transplanting and hardening tissue culture seedlings of rabdosia nervosa |
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Application publication date: 20181123 |