CN108841946A - A kind of method of Non-invasive detection GJB2 gene mutation, kit and preparation method thereof - Google Patents
A kind of method of Non-invasive detection GJB2 gene mutation, kit and preparation method thereof Download PDFInfo
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- CN108841946A CN108841946A CN201810480517.3A CN201810480517A CN108841946A CN 108841946 A CN108841946 A CN 108841946A CN 201810480517 A CN201810480517 A CN 201810480517A CN 108841946 A CN108841946 A CN 108841946A
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- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
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Abstract
The present invention relates to a kind of method of Non-invasive detection GJB2 gene mutation, kits and preparation method thereof, which is characterized in that including steps are as follows:(1) using subject's plasma dna as template;(2) PCR is carried out to expand in advance;(3) Index PCR amplification is carried out to pre- amplified production;(4) after carrying out library Quality Control to PCR extension product, the sequencing of 150bp both-end is carried out in Illumina NextSeq;(5) sequence information of sequencing is subjected to bioinformatic analysis and obtains genetic mutation data.The invention further relates to related kits.
Description
Technical field
The present invention relates to gene diagnosis field more particularly to a kind of methods for detecting gene mutation.
Background technique
The detection of gene mutation is wild type or saltant type for the genotype that can be used for identifying sample to be tested.It can also be with
For detecting the gene appearance of test individual.Than such as whether containing the gene mutation for leading to certain diseases.
Many kinds of lesion detections, fetus genetic state-detection all have important value.It is prominent for the gene of fetus at present
Become screening, be based primarily upon multiple plateform systems such as SNP array, full-length genome snp analysis, cost are carried out to fetus and parent
It is expensive;And routine relies on being sequenced in synthesis for polymerase and is difficult to ensure base accuracy, the base mistake of generation causes to be sequenced
Noise, therefore conventional libraries capture and high-flux sequence not can solve the detection error that makes a variation as caused by amplification and sequencing;And compared with
New circulation monomolecular amplification and weight sequencing technologies (cSMART) technology are cyclized library efficiency and back-to-back primer sites by it
The influence of design.
The discovery of the dissociative DNA of fetal origin in maternal blood, providing for Non-invaive examination fetus genetic state can
Energy.Maternal blood cfDNA is derived from mother and fetus, and fetus dissociative DNA (cffDNA) derives from placental trophoblasts, about
The 10-20% of total cfDNA is accounted for, fragment length is 140-180bp or so.CffDNA is identified from the DNA in mother source
It is still current technology difficult point, it is uneven in particular how detects allele ratio caused by cffDNA in female blood blood plasma
Weighing apparatus.Allele-specific diagnostic PCR, MALDI-TOF mass spectrography, digital pcr and height are mainly passed through to the research of cffDNA in the world at present
Flux sequencing etc..
The noninvasive antenatal detection method of mainstream is based on the detection of fetus variant sites and haplotype detection at present:By to mother
CfDNA carries out site primer in blood blood plasma, then utilizes relative variability dosage (RMD) and relative monomer type dosage (RHDO) point
Analyse the relative amount of fetal DNA in maternal plasma variant sites and haplotype.By the way that there is only the SNP in father to have derived father source portion
The Fetal genome divided, then SNP homozygous using father but mother's heterozygosis is pushed away for the Fetal genome part in mother source
It leads.Papasavva etc. analyzes female blood cfDNA using this method, successfully to its cffDNA genotype and variant sites into
Row analysis.Therefore using the detection to variant sites in cffDNA and haplotype, examining for fetus Disease-causing gene can be effectively improved
Disconnected accuracy.
The methodological study of prenatal gene diagnosis noninvasive for hereditary hearing impairment only has only a few report in the world at present.
Since there are multiple Indel variant sites in the variation of deaf hot spot, (c.299delAT, c.235delC, c.176_ such as GJB2
191del16), different plateform systems still has various problems to its cfDNA detection.
It is necessary to for the technology for developing a kind of more convenient and reliable suitable Non-invasive detection low copy number genes mutation.
Summary of the invention
Therefore there are problem and demand, inventor has developed a kind of fast and accurately monomolecular amplification technology in above-mentioned field,
It joined UMI (Unique Molecular Identifier) molecular label on the basis of conventional multiplex amplification, utilize UMI
Noise reduction is carried out to two generation sequencing datas, thus accurately detection low copy number variation:Concrete scheme is as follows:
A method of detection gene mutation, including steps are as follows:
(1) using subject's plasma dna as template;
(2) PCR is carried out to expand in advance;
(3) Index PCR bis- times amplifications are carried out to pre- amplified production;
(4) library Quality Control is carried out to bis- amplified production products of PCR, then carries out 150bp in Illumina NextSeq
Both-end sequencing;
(5) jointing in the sequence of sequencing is removed, is then spliced into a sequence and obtains primary template sequence;It will be former
Beginning template sequence is compared with human genome, by comparing the UMI molecular label sequence of primary template sequence, is counted unique
Template sequence set;Using unique template sequence, genome covering is calculated, for assessing library specificities;Pass through calculating
The ration statistics of mutant nucleotide sequence and canonical sequence go out detection zone somatic cell gene mutation rate;
It is characterized in that:
The primer used in pre- amplification is the mixture of multipair primer, and the multipair primer is used for specific amplification area to be measured
Different subgoal regions in domain;
All forward primers are followed successively by from 5 ' ends to 3 ' ends containing joint sequence A, UMI molecular label sequence and suborder
Mark region-specific primers sequence;
All reverse primers are followed successively by from 5 ' ends to 3 ' ends containing joint sequence B, UMI molecular label sequence and suborder
Mark region-specific primers sequence;
Joint sequence A in all primer pairs is mutually the same, and joint sequence B is mutually the same, joint sequence A and connector sequence
It arranges not identical between B;
The region to be measured include 1 or several specific gene order regions, the subgoal region refer to one it is specific
The DNA fragmentation of different zones in gene.
A subgoal region in every a pair of primer amplification gene, the UMI molecular label sequence for including between primer pair
Difference, for guaranteeing that the UMI molecular label sequence in the DNA cloning segment in same subgoal region is identical;?
UMI molecular label sequence between the DNA cloning segment in different subgoal regions is differing from each other;
In the Index PCR bis- times amplifications, the secondary amplimer is followed successively by connector sequence from 3 ' ends to 5 ' ends
Column, general sequence measuring joints sequence, wherein side primer also includes one section between joint sequence, general sequence measuring joints sequence
Index sequence;3 ' ends of the forward primer of the secondary amplimer and reverse primer are respectively the joint sequence A and described
The all or part of joint sequence B;The Index sequence is for indicating different samples, and different samples is using different
Index sequence, the secondary amplimer for same sample use same Index sequence.
The above method, it is characterised in that:The joint sequence A, joint sequence A length be 8-15bp, UMI molecule mark
Label sequence length is 5-8bp
The above method, it is characterised in that:Before carrying out the Index PCR amplification, PCR expand in advance obtained by DNA into
Row magnetic beads for purifying;
The above method, it is characterised in that:After carrying out the Index PCR amplification, magnetic is carried out to amplification gained DNA
Pearl purifying.
For detecting the kit of gene mutation, which is characterized in that comprising being expanded in region to be measured in advance for specificity
Multiple primer pairs in different subgoal regions;
All forward primers are followed successively by from 5 ' ends to 3 ' ends containing joint sequence A, UMI molecular label sequence and suborder
Mark region-specific primers sequence;
All reverse primers are followed successively by from 5 ' ends to 3 ' ends containing joint sequence B, UMI molecular label sequence and suborder
Mark region-specific primers sequence;
Joint sequence A in all primer pairs is mutually the same, and joint sequence B is mutually the same, joint sequence A and connector sequence
It arranges not identical between B;
UMI molecular label sequence in the DNA fragmentation of same source is identical;And between the DNA fragmentation of separate sources
UMI molecular label sequence is differing from each other;
The region to be measured include 1 or several specific gene order regions, the subgoal region refer to one it is specific
Different location region in gene.
Mentioned reagent box, which is characterized in that also draw comprising the secondary amplification for being expanded to the pre- amplified production
Object, the secondary amplimer are followed successively by joint sequence, general sequence measuring joints sequence from 3 ' ends to 5 ' ends, and wherein side is drawn
Object also includes one section of Index sequence between joint sequence, general sequence measuring joints sequence;The forward direction of the secondary amplimer is drawn
3 ' ends of object and reverse primer are respectively all or part of the joint sequence A and the joint sequence B;The Index sequence
Column use different Index sequences, the secondary amplimer for same sample for indicating different samples, different samples
Using same Index sequence.
Mentioned reagent box, it is characterised in that:The joint sequence A, joint sequence A length be 8-15bp, UMI molecule
Sequence label length is 5-7bp.
For detecting the preparation method of the kit of gene mutation, which is characterized in that including synthesize and/or assemble have with
Multiple primer pairs of lower sequence signature,
All forward primers are followed successively by from 5 ' ends to 3 ' ends containing joint sequence A, UMI molecular label sequence and suborder
Mark region-specific primers sequence;
All reverse primers are followed successively by from 5 ' ends to 3 ' ends containing joint sequence B, UMI molecular label sequence and suborder
Mark region-specific primers sequence;
Joint sequence A in all primer pairs is mutually the same, and joint sequence B is mutually the same, joint sequence A and connector sequence
It arranges not identical between B;
UMI molecular label sequence in the DNA fragmentation of same source is identical;And between the DNA fragmentation of separate sources
UMI molecular label sequence is differing from each other;
The multiple primer pair expands the different subgoal regions in region to be measured for specificity in advance;The region to be measured
Comprising 1 or several specific gene order regions, the subgoal region refer to the different location area in a specific gene
Domain.
Above-mentioned preparation method, which is characterized in that also comprising synthesis and/or assembling for expanding the pre- amplified production
The secondary amplimer increased, the secondary amplimer are followed successively by joint sequence, general sequence measuring joints sequence from 3 ' ends to 5 ' ends
Column, wherein side primer also includes one section of Index sequence between joint sequence, general sequence measuring joints sequence;The secondary amplification
The forward primer of primer and 3 ' ends of reverse primer are respectively whole or the portion of the joint sequence A and joint sequence B
Point;The Index sequence uses different Index sequences for indicating different samples, different samples, is used for same sample
Secondary amplimer use same Index sequence.
Above-mentioned preparation method, it is characterised in that:The joint sequence A, joint sequence A length be 8-15bp, UMI point
Subtab sequence length is 5-7bp.
Application of the above method in GJB2 gene mutation and SLC26A4 detection in Gene Mutation derives following technical scheme
A kind of Non-invasive detection GJB2 and/or the method for SLC26A4 gene mutation, including steps are as follows:
(1) using subject's plasma dna as template;
(2) PCR is carried out to expand in advance;
(3) Index PCR bis- times amplifications are carried out to pre- amplified production;
(4) library Quality Control is carried out to bis- amplified production products of PCR, then carries out 150bp in Illumina NextSeq
Both-end sequencing;
(5) jointing in the sequence of sequencing is removed, is then spliced into a sequence and obtains primary template sequence, it will be former
Beginning template sequence is compared with human genome, by comparing the UMI molecular label sequence of primary template sequence, is counted unique
Template sequence set;Using unique template sequence, genome covering is calculated, for assessing library specificities;Pass through calculating
The ration statistics of mutant nucleotide sequence and canonical sequence go out detection zone somatic cell gene mutation rate;
It is characterized in that:
The mixing of the 26 pairs of primers constituted in pre- amplification using primer shown in Seq ID No.1-46,89-91,94-96
Object is used for the different subgoal regions of specific amplification GJB2 gene;
The mixing of the 23 pairs of primers constituted in pre- amplification using primer shown in Seq ID No.47-88,92-93,97-98
Object is used for the different subgoal regions of specific amplification SLC26A4 gene;
Alternatively, the mixture of the 49 pairs of primers constituted in pre- amplification using primer shown in Seq ID No.1-98 is for same
When specific amplification GJB2 gene and SLC26A4 gene different subgoal regions;To simultaneously detect GJB2 gene and
The variation of SLC26A4 gene;
The region to be measured include 1 or several specific gene order regions, the subgoal region refer to one it is specific
The DNA fragmentation of different zones in gene;
A subgoal region in every a pair of primer amplification gene, the UMI molecular label sequence for including between primer pair
Difference, for guaranteeing that the UMI molecular label sequence in the DNA cloning segment in same subgoal region is identical;?
UMI molecular label sequence between the DNA cloning segment in different subgoal regions is differing from each other;
In the Index PCR bis- times amplifications, using forward and reverse primer shown in Seq ID No.99 and 100.
The above method, it is characterised in that:Before carrying out the Index PCR amplification, PCR expand in advance obtained by DNA into
Row magnetic beads for purifying;
The above method, it is characterised in that:After carrying out the Index PCR amplification, magnetic is carried out to amplification gained DNA
Pearl purifying.
Kit for Non-invasive detection GJB2 and/or SLC26A4 gene mutation, which is characterized in that
Mixture including 26 pairs of primers that primer shown in Seq ID No.1-46,89-91,94-96 is constituted, is used for
The different subgoal regions of specific amplification GJB2 gene;
The mixture of 23 pairs of primers including the composition of primer shown in Seq ID No.47-88,92-93,97-98 is for spy
The different subgoal regions of specific amplification SLC26A4 gene;
Alternatively, including the mixture for 49 pairs of primers that primer shown in Seq ID No.1-98 is constituted for specificity simultaneously
Expand the different subgoal regions of GJB2 gene and SLC26A4 gene;To detect GJB2GJB2 gene and SLC26A4 simultaneously
The variation of gene.
Mentioned reagent box, which is characterized in that also draw comprising the secondary amplification for being expanded to the pre- amplified production
Object, forward and reverse primer of the secondary amplimer is as shown in Seq ID No.99 and 100.
For detecting the preparation method of the kit of GJB2 gene mutation, which is characterized in that including synthesizing and/or assembling
With Seq ID No.1-46,89-91, the primer of nucleotide sequence shown in 94-96.
Or the preparation method of the kit for detecting SLC26A4 gene mutation, which is characterized in that including synthesis and/or
Assemble the primer with nucleotide sequence shown in Seq ID No.47-88,92-93,97-98.
Or the preparation method of the kit for detecting GJB2 gene and SLC26A4 gene mutation, which is characterized in that packet
Including synthesis and/or assembling has the primer of nucleotide sequence shown in Seq ID No.1-98.
Above-mentioned preparation method, which is characterized in that also comprising synthesis and/or assembling for expanding the pre- amplified production
The secondary amplimer increased, forward and reverse primer of the secondary amplimer is as shown in Seq ID No.99 and 100.
The detection method of gene mutation provided according to the present invention, for specific testing gene, those skilled in the art can
To design the specific primer sequence that screening amplification is directed to different subgoal regions according to each subgoal region of selection, make
Minimum can be interfered with each other in same reaction by obtaining between these primer pairs, guarantee detection accuracy and sensitivity.Further according to drawing
The rule that is typically designed of object designs or selects the joint sequence for being suitble to all primer pairs, is different primers to design UMI molecule mark
Sequence is signed, so that the primer of the DNA fragmentation of amplification same source uses identical UMI molecular label sequence, and difference is expanded and comes
The pre- amplification primer sets for specific testing gene can be obtained in UMI molecule between the primer of the DNA fragmentation in source.It can be used for
Maternal blood cfDNA detects the monogenic diseases such as the genetic state, such as deaf gene of fetus, and is swum by oncogene
From DNA gene cancer detection.
Gene mutation method of the invention will be had identical by introducing UMI molecular label by analysis of biological information
The amplification sequencing product of UMI sequence merges, and significantly reduces by archaeal dna polymerase amplification procedure and two generation sequencing procedures
Introduced mistake and false positive rate is realized by noise reduction and is accurately detected to low copy number variation.
Experimental example is by detecting the known mutational site GJB2 and SLC26A4, the reliability of verification method.
Detailed description of the invention
Fig. 1 is the principle of the present invention schematic diagram:
Plasma dna is expanded in advance using mix primer first, it is therefore an objective to locus products needed for expanding;
Then pre- amplified production DNA is purified, it is therefore an objective to enzyme and ion in removal system;
Index PCR bis- times amplifications are carried out for pre- amplified production, are fabricated to the library that can be used for high-flux sequence;
Bioinformatic analysis finally is carried out to library and obtains gene mutation data.
Fig. 2 bioinformatic analysis process example
Sequence (i.e. same samples sources) with identical molecular label is carried out being assembled into unique template sequence
Specific embodiment
In the embodiment of the present invention, a kind of method for detecting gene mutation is provided, including steps are as follows:
A method of detection gene mutation, including steps are as follows:
(1) using subject's plasma dna as template;
(2) PCR is carried out to expand in advance;
(3) Index PCR bis- times amplifications are carried out to pre- amplified production;
(4) library Quality Control is carried out to bis- amplified production products of PCR, then carries out 150bp in Illumina NextSeq
Both-end sequencing;
(5) jointing in the sequence of sequencing is removed, is then spliced into a sequence and obtains primary template sequence;It will be former
Beginning template sequence is compared with human genome, by comparing the UMI molecular label sequence of primary template sequence, is counted unique
Template sequence set;Using unique template sequence, genome covering is calculated, for assessing library specificities;Pass through calculating
The ration statistics of mutant nucleotide sequence and canonical sequence go out detection zone somatic cell gene mutation rate;
It is characterized in that:
The primer used in pre- amplification is the mixture of multipair primer, and the multipair primer is used for specific amplification area to be measured
Different subgoal regions in domain;
All forward primers are followed successively by from 5 ' ends to 3 ' ends containing joint sequence A, UMI molecular label sequence and suborder
Mark region-specific primers sequence;
All reverse primers are followed successively by from 5 ' ends to 3 ' ends containing joint sequence B, UMI molecular label sequence and suborder
Mark region-specific primers sequence;
Joint sequence A in all primer pairs is mutually the same, and joint sequence B is mutually the same, joint sequence A and connector sequence
It arranges not identical between B;
The region to be measured include 1 or several specific gene order regions, the subgoal region refer to one it is specific
The DNA fragmentation of different zones in gene.
A subgoal region in every a pair of primer amplification gene, the UMI molecular label sequence for including between primer pair
Difference, for guaranteeing that the UMI molecular label sequence in the DNA cloning segment in same subgoal region is identical;?
UMI molecular label sequence between the DNA cloning segment in different subgoal regions is differing from each other;
In the Index PCR bis- times amplifications, use secondary amplimer is followed successively by connector from 3 ' ends to 5 ' ends
Sequence, general sequence measuring joints sequence, wherein side primer also includes one section between joint sequence, general sequence measuring joints sequence
Index sequence;3 ' ends of the forward primer of the secondary amplimer and reverse primer are respectively the joint sequence A and described
Joint sequence B;The Index sequence uses different Index sequences for indicating different samples, different samples, for same
The secondary amplimer of one sample uses same Index sequence.
For specific testing gene, those skilled in the art can design according to the subgoal region of selection and expand the area
The subgoal region-specific primers sequence in domain is typically designed the joint sequence that rule designs or selection is suitable according to primer,
And be different primer pair Random Design UMI molecular label sequences, the pre- amplimer for specific testing gene can be obtained
Group.Such as the monogenic diseases such as genetic state, such as deaf gene that can be used for maternal blood cfDNA detection fetus, Yi Jitong
Cross oncogene dissociative DNA gene cancer detection.
In some embodiments, the joint sequence A, joint sequence A length be 8-15bp, UMI molecular label sequence is long
Degree is 5-8bp
In some embodiments, before carrying out the Index PCR bis- times amplifications, expands gained DNA in advance to PCR and carry out
Magnetic beads for purifying;
In some embodiments, after carrying out the Index PCR amplification, magnetic beads for purifying is carried out to amplification gained DNA.
Implementation of the invention further includes the kit provided for detecting gene mutation, which is characterized in that comprising being used for
Specificity expands multiple primer pairs in the different subgoal regions in region to be measured in advance;
All forward primers are followed successively by from 5 ' ends to 3 ' ends containing joint sequence A, UMI molecular label sequence and suborder
Mark region-specific primers sequence;
All reverse primers are followed successively by from 5 ' ends to 3 ' ends containing joint sequence B, UMI molecular label sequence and suborder
Mark region-specific primers sequence;
Joint sequence A in all primer pairs is mutually the same, and joint sequence B is mutually the same, joint sequence A and connector sequence
It arranges not identical between B;
The region to be measured include 1 or several specific gene order regions, the subgoal region refer to one it is specific
Different location region in gene;
A subgoal region in every a pair of primer amplification gene, the UMI molecular label sequence for including between primer pair
Difference, for guaranteeing that the UMI molecular label sequence in the DNA cloning segment in same subgoal region is identical;?
UMI molecular label sequence between the DNA cloning segment in different subgoal regions is differing from each other.
In some kits, also comprising secondary amplimer for being expanded to the pre- amplified production, described two
Secondary amplimer is followed successively by joint sequence, general sequence measuring joints sequence from 3 ' ends to 5 ' ends, and wherein side primer is in connector sequence
It also include one section of Index sequence between column, general sequence measuring joints sequence;The forward primer of the secondary amplimer and reversely draw
3 ' ends of object are respectively the joint sequence A and joint sequence B;The Index sequence is for indicating different samples, no
Same sample uses different Index sequences, and the secondary amplimer for same sample uses same Index sequence.
Forward and reverse 3 ' sequence of primer is respectively that (5 ' terminal sequences of secondary amplimer are directed to joint sequence A and joint sequence B
The library of Illumina sequenator requires to be designed)
In some kits, the preferably described joint sequence A, joint sequence A length be 8-15bp, UMI molecular label sequence
Column length is 5-7bp.
Implementation of the invention further includes providing the preparation method of the kit for detecting gene mutation, which is characterized in that
Including synthesizing and/or assembling multiple primer pairs with following sequence signature,
All forward primers are followed successively by from 5 ' ends to 3 ' ends containing joint sequence A, UMI molecular label sequence and suborder
Mark region-specific primers sequence;
All reverse primers are followed successively by from 5 ' ends to 3 ' ends containing joint sequence B, UMI molecular label sequence and suborder
Mark region-specific primers sequence;
Joint sequence A in all primer pairs is mutually the same, and joint sequence B is mutually the same, joint sequence A and connector sequence
It arranges not identical between B;
The region to be measured include 1 or several specific gene order regions, the subgoal region refer to one it is specific
Different location region in gene;
A subgoal region in every a pair of primer amplification gene, the UMI molecular label sequence for including between primer pair
Difference, for guaranteeing that the UMI molecular label sequence in the DNA cloning segment in same subgoal region is identical;?
UMI molecular label sequence between the DNA cloning segment in different subgoal regions is differing from each other.
In some preparation methods, also comprising synthesizing and/or assembling two for being expanded to the pre- amplified production
Secondary amplimer, use secondary amplimer is followed successively by joint sequence, general sequence measuring joints sequence from 3 ' ends to 5 ' ends,
Wherein side primer also includes one section of Index sequence between joint sequence, general sequence measuring joints sequence;The secondary amplification is drawn
The forward primer of object and 3 ' ends of reverse primer are respectively the joint sequence A and joint sequence B;The Index sequence
For indicating different samples, different samples uses different Index sequences, and the secondary amplimer for same sample is adopted
With same Index sequence.
In some preparation methods, the joint sequence A, joint sequence A length be 8-15bp, UMI molecular label sequence
Column length is 5-7bp.
Application Example detects GJB2 and SLC26A4 gene mutation
GJB2 gene encodes inserted by connexin 26, and overall length 678bp contains two exons.Its protein expression in inner ear,
To maintaining in cochlea, normally current potential plays a significant role.Its gene mutation can lead to recessive hereditary deafness and often
Autosomal dominant hereditary hearing impairment, deafness caused by being mutated account for about the half of whole recessive hereditary deafnesses, account for and distribute
60% in case.The SLC26A4 assignment of genes gene mapping is on chromosome 7q22.3, overall length 2343bp.It encodes albumen pendrin master
It to be expressed in inner ear, thyroid gland and kidney, function is related to chloride ion and carbonic acid hydrogen ion transhipment.SLC26A4 gene is prominent
Change can lead to Pendred syndrome and DFNB4 type non-syndrome ear.Gao Z is using SNPscan technology to 695 Chinese ears
The deafness patient of deaf patient's screening discovery 38.7% is caused by GJB2 and SLC26A4 genetic mutation, and it was found that at this
Mutation is in apparent founder effect in a little genes, therefore is had to the research of hereditary hearing impairment caused by the two genes important
Clinical meaning.
Therefore, clinically there is an urgent need to a kind of quick, the efficient method for detecting GJB2 and SLC26A4 gene mutation.
The present inventor is when studying the detection of piece segment DNA, it was found that a kind of detection method of new DNA fragmentation, i.e., by drawing to amplification
Object adds molecular label, then expands to DNA fragmentation.On this basis, special in conjunction with second generation high throughput sequencing technologies
It is not improved according to GJB2 and SLC26A4 gene pairs this method, and is especially devised preferably according to GJB2 and SLC26A4
Primer, develop a kind of method that the mutation of GJB2 and SLC26A4 gene is sequenced and is analyzed by plasma dna with
And its kit.
The methodological study of prenatal gene diagnosis noninvasive for hereditary hearing impairment only has only a few report in the world at present.
Since there are multiple Indel variant sites in the variation of deaf hot spot, (c.299delAT, c.235delC, c.176_ such as GJB2
191del16), different plateform systems still has various problems to its cfDNA detection.It is such as multiple flat based on SNP array
Platform system carries out full-length genome snp analysis to fetus and parent, expensive;It is conventional to rely on being sequenced in synthesis for polymerase
It is difficult to ensure base accuracy, the sequencing noise of base mistake will be generated, lead to conventional libraries capture and high-flux sequence still not
It can solve in variation by expanding and sequencing causes a deviation;Newer circulation monomolecular amplification and weight sequencing technologies (cSMART)
Technology is influenced by the design that it is cyclized library efficiency and back-to-back primer sites.Method of the invention has well solved this
One problem joined UMI (Unique Molecular Identifier) molecular label on the basis of conventional multiplex amplification,
Noise reduction is carried out to two generation sequencing datas using UMI, to detect to low copy number variation.
Pass through the reliability of template verification method known to the detection mutational site GJB2 and SLC26A4
Cell line dna:(army of ground force will be derived from containing multiple DNA samples that GJB2 and SLC26A4 gene hot makes a variation First affiliated hospital's DNA sample library is learned by medical university) ultrasonication, 166 ± 10bp segment, which is recycled, by preset ratio is mixed into normal person's blood
Slurry DNA obtains sample to be tested known to mutant proportion (calculating according to positive DNA concentration)):
The GJB2 hotspot mutation ratio expection of the sample is as follows
Mutational site | It is expected that mutant proportion |
c.235delC | 5.50% |
c.299_300delAT | 6.20% |
c.176_191del16 | 9.30% |
The SLC26A4 hotspot mutation ratio expection of the sample is as follows:
Mutational site | It is expected that mutant proportion |
NM_000441:c.919-2A>G | 2.70% |
NM_000441:c.2168A>G | 4.50% |
Test the sensitivity and stability of the method for the present invention.
Pre- amplimer MIX:By in first group in table 1 primer or second group in primer or first and second groups
Whole primer sequences is added in 1.5ml EP pipe
Table 1 expands primer sets in advance
The mixture for 26 pairs of primers that primer shown in Seq ID No.1-46,89-91,94-96 is constituted, for special
Property amplification GJB2 gene different subgoal regions SNP site and hot spot variation;
The mixture for 23 pairs of primers that primer shown in Seq ID No.47-88,92-93,97-98 is constituted is for specificity
Expand the SNP site and hot spot variation in the different subgoal regions of SLC26A4 gene;
" NNNNNN " in every primer is a kind of A/G/C/T random sequence of synthesis, that is, UMI " unique
molecular identifier".UMI in each pair of primer is different from each other.
Note:5 ' terminal sequences " CCTACACGACGCTCTTCCGATCT " in forward primer are joint sequence A;Reversely draw
5 ' terminal sequence of object " TCAGACGTGTGCTCTTCCGATCT " is that B.3.PCR joint sequence expands in advance
Prepare following reaction mixture
DNA | 24.8ul |
Buffer 10X | 3ul |
dNTP(2.5mM) | 1ul |
Primer Mix | 1ul |
Taq polymerase | 0.2ul |
30ul |
It is reacted in PCR instrument:
The purification and recovery DNA sample on Beckman magnetic bead, and eluted in the sterile dH20 or elution buffer of 25 μ 1.
4.Index PCR amplification
2 index primer of table
99 | Primer For: | 5-AATGATACGGCGACCACCGAGATCTACACACACTCTTTCCCTACACGACGC-3 |
100 | Index Primer Rev: | 5-CAAGCAGAAGACGGCATACGAGATCTCTTAATTGACTGGAGTTCAGACGTGTGCT-3 |
5 ' ends and 5 ' terminal sequence of Index Primer Rev disclose general survey for Illumina company in Primer F
Sequence connector;In reverse primer "CTCTTAAT " isIndex sequence, for distinguishing different samples, 3 ' terminal sequences
" CCTACACGACGC ", it is the connector A and connector B of primer in table 1 that Index Primer Rev 3 ', which holds " TCAGACGTGTGCT ",
5 ' terminal sequences.
It is reacted in PCR instrument:
The purification and recovery DNA sample on Beckman magnetic bead, and eluted in the sterile dH20 or elution buffer of 25 μ 1
Obtain library.
By the library made by the way that after the Quality Control of Qubit detectable concentration, Illumina NextSeq carries out 150bp both-end
Sequencing (operating procedure is operated according to Illumina company operating instruction).
The sequence of high-flux sequence is spliced into a sequence according to repeat region, jointing is removed, sequence is restored
Primary template sequence is returned, by template matching to human genome (hg19), by comparing the molecular label sequence of template sequence,
Count unique template sequence set, such as Fig. 2:Sequence with identical molecular label is carried out being assembled into unique template
Sequence.
Using unique template sequence, genome covering is calculated, for assessing library specificities, is mutated sequence by calculating
The ration statistics detection zone somatic mutation of column and canonical sequence.
After tradition is without the primer amplification of molecular label, sequencing can not distinguish the different sequences of its identical source.We
By introducing UMI molecular label, the sequence with identical source UMI is merged by analysis of biological information, greatly
The mistake introduced by archaeal dna polymerase amplification procedure and two generation sequencing procedures is reduced, we can realize by noise reduction copies to low
The variation of shellfish number is accurately detected.
It is as follows for GJB2 gene hot mutant site testing result:
Mutational site | Mutant proportion | Testing result |
c.235delC | 5.59% | Detection |
c.299_300delAT | 6.14% | Detection |
c.176_191del16 | 9.44% | Detection |
It is as follows for SLC26A4 gene hot mutant site testing result:
Mutational site | Mutant proportion | Testing result |
NM_000441:c.919-2A>G | 2.87% | Detection |
NM_000441:c.2168A>G | 4.56% | Detection |
Upper table is the testing result of the low ratio hot spot mutation of two genes, is consistent with expected results.
Meanwhile the testing result of method of the invention and the testing result of other methods are also highly consistent.Illustrate the present invention
Method can accurately detect low-copy mutation.
For same sample, one of site mutation ratio is detected using ddPCR method, as a result with
The method of the present invention testing result is consistent.But ddPCR can only be detected for single locus mutation.
Under certain condition, for different known mutations, designing primer appropriate can be by PCR amplification method knot
It closes Sanger sequencing and directly reaches the purpose for distinguishing saltant type and wild type gene.This method is mainly for peripheral blood sample.It adopts
It is combined with PCR amplification and detects peripheral blood sample mutation type for known site, then carry out blood plasma simulation with the method for the present invention
DNA detection.
Finally, it is stated that the above examples are only used to illustrate the technical scheme of the present invention and are not limiting, although referring to compared with
Good embodiment describes the invention in detail, those skilled in the art should understand that, it can be to skill of the invention
Art scheme is modified or replaced equivalently, and without departing from the objective and range of technical solution of the present invention, should all be covered at this
In the scope of the claims of invention.
SEQUENCE LISTING
<110>The first affiliated hospital of army medical university of ground force of the Chinese People's Liberation Army
<120>A kind of method of Non-invasive detection GJB2 gene mutation, kit and preparation method thereof
<130> P1830152CN-CN-LJY-CQ-LSH
<160> 100
<170> PatentIn version 3.3
<210> 1
<211> 51
<212> DNA
<213> Artificial sequence
<220>
<223> rs7999318_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 1
cctacacgac gctcttccga tctnnnnnna agtgacccgc tgtgcatgta t 51
<210> 2
<211> 51
<212> DNA
<213> Artificial sequence
<220>
<223> rs4769954_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 2
cctacacgac gctcttccga tctnnnnnna caggagatgc aggcagtcaa c 51
<210> 3
<211> 51
<212> DNA
<213> Artificial sequence
<220>
<223> rs9509170_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 3
cctacacgac gctcttccga tctnnnnnng atcccatcag aaggaccctg t 51
<210> 4
<211> 54
<212> DNA
<213> Artificial sequence
<220>
<223> rs1537788_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 4
cctacacgac gctcttccga tctnnnnnng agatgcttca aaatacagga atgg 54
<210> 5
<211> 54
<212> DNA
<213> Artificial sequence
<220>
<223> rs9552134_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 5
cctacacgac gctcttccga tctnnnnnnt gcagagatga aattgtactt gcag 54
<210> 6
<211> 50
<212> DNA
<213> Artificial sequence
<220>
<223> rs12585595_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 6
cctacacgac gctcttccga tctnnnnnnc cagccattct ctcccatgtt 50
<210> 7
<211> 55
<212> DNA
<213> Artificial sequence
<220>
<223> rs9509191_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 7
cctacacgac gctcttccga tctnnnnnnt gagctacaat cgaactcatc tttcc 55
<210> 8
<211> 51
<212> DNA
<213> Artificial sequence
<220>
<223> rs11147616_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 8
cctacacgac gctcttccga tctnnnnnnc tctgcttcag gggcttgttc t 51
<210> 9
<211> 52
<212> DNA
<213> Artificial sequence
<220>
<223> rs9552150_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 9
cctacacgac gctcttccga tctnnnnnnc aggtaggacc caagatttgt gc 52
<210> 10
<211> 51
<212> DNA
<213> Artificial sequence
<220>
<223> rs9509098_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 10
cctacacgac gctcttccga tctnnnnnng gagaagatgc ctcctccatt c 51
<210> 11
<211> 49
<212> DNA
<213> Artificial sequence
<220>
<223> rs870728_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 11
cctacacgac gctcttccga tctnnnnnng actgcctgac ccctccaac 49
<210> 12
<211> 51
<212> DNA
<213> Artificial sequence
<220>
<223> rs35967187_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 12
cctacacgac gctcttccga tctnnnnnng agtgaccacc ctggcacata a 51
<210> 13
<211> 52
<212> DNA
<213> Artificial sequence
<220>
<223> rs2313485_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 13
cctacacgac gctcttccga tctnnnnnnc catcagatgt ggcattcaga aa 52
<210> 14
<211> 51
<212> DNA
<213> Artificial sequence
<220>
<223> rs7327952_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 14
cctacacgac gctcttccga tctnnnnnna ccatcaccca tcacccctaa t 51
<210> 15
<211> 52
<212> DNA
<213> Artificial sequence
<220>
<223> rs8000719_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 15
cctacacgac gctcttccga tctnnnnnng gttgtgacat aaacagggga ca 52
<210> 16
<211> 48
<212> DNA
<213> Artificial sequence
<220>
<223> rs9550644_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 16
cctacacgac gctcttccga tctnnnnnnt gctgggcagg atggagac 48
<210> 17
<211> 50
<212> DNA
<213> Artificial sequence
<220>
<223> rs945368_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 17
cctacacgac gctcttccga tctnnnnnng atttttctgg gtccccaagc 50
<210> 18
<211> 50
<212> DNA
<213> Artificial sequence
<220>
<223> rs9506430_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 18
cctacacgac gctcttccga tctnnnnnnc agactgctca agccctctcc 50
<210> 19
<211> 51
<212> DNA
<213> Artificial sequence
<220>
<223> rs6490533_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 19
cctacacgac gctcttccga tctnnnnnna ctctctgcgt gaggtgagtg g 51
<210> 20
<211> 50
<212> DNA
<213> Artificial sequence
<220>
<223> rs2872371_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 20
cctacacgac gctcttccga tctnnnnnnc ggctctgtgc taggaaccag 50
<210> 21
<211> 51
<212> DNA
<213> Artificial sequence
<220>
<223> rs9552160_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 21
cctacacgac gctcttccga tctnnnnnnt tggcacagat gcagacctct t 51
<210> 22
<211> 52
<212> DNA
<213> Artificial sequence
<220>
<223> rs9550642_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 22
cctacacgac gctcttccga tctnnnnnng gaaccttgag tactgggctg aa 52
<210> 23
<211> 51
<212> DNA
<213> Artificial sequence
<220>
<223> rs7988514_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 23
cctacacgac gctcttccga tctnnnnnnt ccctcaggtc ccatttagct c 51
<210> 24
<211> 51
<212> DNA
<213> Artificial sequence
<220>
<223> rs7999318_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 24
tcagacgtgt gctcttccga tctnnnnnng agagtggaga gggagctgag g 51
<210> 25
<211> 51
<212> DNA
<213> Artificial sequence
<220>
<223> rs4769954_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 25
tcagacgtgt gctcttccga tctnnnnnng ggacaggaga ccctgtgttt t 51
<210> 26
<211> 51
<212> DNA
<213> Artificial sequence
<220>
<223> rs9509170_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 26
tcagacgtgt gctcttccga tctnnnnnng agtccccttc tccctgaggt t 51
<210> 27
<211> 58
<212> DNA
<213> Artificial sequence
<220>
<223> rs1537788_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 27
tcagacgtgt gctcttccga tctnnnnnna aaacaaaata taagttttag gggctgaa 58
<210> 28
<211> 51
<212> DNA
<213> Artificial sequence
<220>
<223> rs9552134_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 28
tcagacgtgt gctcttccga tctnnnnnng gtcttttgtg ggggttgaga c 51
<210> 29
<211> 53
<212> DNA
<213> Artificial sequence
<220>
<223> rs12585595_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 29
tcagacgtgt gctcttccga tctnnnnnnc acagcagacc tcaacagaca gaa 53
<210> 30
<211> 51
<212> DNA
<213> Artificial sequence
<220>
<223> rs9509191_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 30
tcagacgtgt gctcttccga tctnnnnnng ccctacactt ccattcccat c 51
<210> 31
<211> 47
<212> DNA
<213> Artificial sequence
<220>
<223> rs11147616_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 31
tcagacgtgt gctcttccga tctnnnnnnc ctggccaaca accacca 47
<210> 32
<211> 51
<212> DNA
<213> Artificial sequence
<220>
<223> rs9552150_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 32
tcagacgtgt gctcttccga tctnnnnnnt ttacaaatgc cagccaattc c 51
<210> 33
<211> 51
<212> DNA
<213> Artificial sequence
<220>
<223> rs9509098_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 33
tcagacgtgt gctcttccga tctnnnnnnt ggttgtacgg gtattgcatg a 51
<210> 34
<211> 59
<212> DNA
<213> Artificial sequence
<220>
<223> rs870728_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 34
tcagacgtgt gctcttccga tctnnnnnnt gtaatcacta tagacagacc cttttcagc 59
<210> 35
<211> 50
<212> DNA
<213> Artificial sequence
<220>
<223> rs35967187_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 35
tcagacgtgt gctcttccga tctnnnnnng cgagggaggt ctcggataag 50
<210> 36
<211> 51
<212> DNA
<213> Artificial sequence
<220>
<223> rs2313485_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 36
tcagacgtgt gctcttccga tctnnnnnnt ttccccaact attccacagc a 51
<210> 37
<211> 52
<212> DNA
<213> Artificial sequence
<220>
<223> rs7327952_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 37
tcagacgtgt gctcttccga tctnnnnnng atcagcagtt gtcagaggct gt 52
<210> 38
<211> 51
<212> DNA
<213> Artificial sequence
<220>
<223> rs8000719_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 38
tcagacgtgt gctcttccga tctnnnnnna aggaaaatgg tgcatgccta c 51
<210> 39
<211> 48
<212> DNA
<213> Artificial sequence
<220>
<223> rs9550644_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 39
tcagacgtgt gctcttccga tctnnnnnng cgccacttcc ctgttgaa 48
<210> 40
<211> 51
<212> DNA
<213> Artificial sequence
<220>
<223> rs945368_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 40
tcagacgtgt gctcttccga tctnnnnnng gcataggtgg attgccaatt t 51
<210> 41
<211> 52
<212> DNA
<213> Artificial sequence
<220>
<223> rs9506430_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 41
tcagacgtgt gctcttccga tctnnnnnng aggttgctct aatcccacct ga 52
<210> 42
<211> 49
<212> DNA
<213> Artificial sequence
<220>
<223> rs6490533_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 42
tcagacgtgt gctcttccga tctnnnnnnc tgcgtcaacg gctcctatg 49
<210> 43
<211> 49
<212> DNA
<213> Artificial sequence
<220>
<223> rs2872371_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 43
tcagacgtgt gctcttccga tctnnnnnnc actggggaaa gggcaaatc 49
<210> 44
<211> 51
<212> DNA
<213> Artificial sequence
<220>
<223> rs9552160_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 44
tcagacgtgt gctcttccga tctnnnnnnt ccaccaagag gaagaaaagg a 51
<210> 45
<211> 51
<212> DNA
<213> Artificial sequence
<220>
<223> rs9550642_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 45
tcagacgtgt gctcttccga tctnnnnnnt cagaggacag ttccctgttg g 51
<210> 46
<211> 51
<212> DNA
<213> Artificial sequence
<220>
<223> rs7988514_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 46
tcagacgtgt gctcttccga tctnnnnnnc cttttctttt gggcacgttt t 51
<210> 47
<211> 57
<212> DNA
<213> Artificial sequence
<220>
<223> rs28690459_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 47
cctacacgac gctcttccga tctnnnnnna caaaagcctt cttaagttta ggaaaca 57
<210> 48
<211> 56
<212> DNA
<213> Artificial sequence
<220>
<223> rs73421853_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 48
cctacacgac gctcttccga tctnnnnnnt ggtttaaagg agtatctgga gtcaaa 56
<210> 49
<211> 54
<212> DNA
<213> Artificial sequence
<220>
<223> rs17154507_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 49
cctacacgac gctcttccga tctnnnnnnt gacgttatca ggatacggct atct 54
<210> 50
<211> 51
<212> DNA
<213> Artificial sequence
<220>
<223> rs6944998_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 50
cctacacgac gctcttccga tctnnnnnnc ctgggctcac ttgacacaaa c 51
<210> 51
<211> 59
<212> DNA
<213> Artificial sequence
<220>
<223> rs10276013_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 51
cctacacgac gctcttccga tctnnnnnnc cacctattag cttctaaaac aatgtaagg 59
<210> 52
<211> 51
<212> DNA
<213> Artificial sequence
<220>
<223> rs7805114_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 52
cctacacgac gctcttccga tctnnnnnna aaccagtcat gggccttagg a 51
<210> 53
<211> 56
<212> DNA
<213> Artificial sequence
<220>
<223> rs10242548_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 53
cctacacgac gctcttccga tctnnnnnnt ggttacaaac ataaccctgt tattgc 56
<210> 54
<211> 51
<212> DNA
<213> Artificial sequence
<220>
<223> rs6949189_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 54
cctacacgac gctcttccga tctnnnnnnt gcagacgggt ttgaaatttt g 51
<210> 55
<211> 59
<212> DNA
<213> Artificial sequence
<220>
<223> rs1008707_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 55
cctacacgac gctcttccga tctnnnnnnt cactcaactc ttaaaagaat cctacttga 59
<210> 56
<211> 55
<212> DNA
<213> Artificial sequence
<220>
<223> rs78502829_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 56
cctacacgac gctcttccga tctnnnnnnc atgacagcca ttctacagtt tatgc 55
<210> 57
<211> 51
<212> DNA
<213> Artificial sequence
<220>
<223> rs6466197_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 57
cctacacgac gctcttccga tctnnnnnna ttgtccccag gccaataaac t 51
<210> 58
<211> 52
<212> DNA
<213> Artificial sequence
<220>
<223> rs10487275_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 58
cctacacgac gctcttccga tctnnnnnna gggacaggat ccagtagaaa gg 52
<210> 59
<211> 51
<212> DNA
<213> Artificial sequence
<220>
<223> rs3823958_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 59
cctacacgac gctcttccga tctnnnnnnc aaggtagcca gagaagggtg a 51
<210> 60
<211> 54
<212> DNA
<213> Artificial sequence
<220>
<223> rs6980029_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 60
cctacacgac gctcttccga tctnnnnnnt gcacttaaag gtagaggctc agaa 54
<210> 61
<211> 51
<212> DNA
<213> Artificial sequence
<220>
<223> rs4730261_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 61
cctacacgac gctcttccga tctnnnnnnc tctgaggatg gctatggctg t 51
<210> 62
<211> 51
<212> DNA
<213> Artificial sequence
<220>
<223> rs57592817_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 62
cctacacgac gctcttccga tctnnnnnna ggctgccgta gatttggtct g 51
<210> 63
<211> 48
<212> DNA
<213> Artificial sequence
<220>
<223> rs2253856_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 63
cctacacgac gctcttccga tctnnnnnng tgagcccagt cccctgaa 48
<210> 64
<211> 51
<212> DNA
<213> Artificial sequence
<220>
<223> rs982663_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 64
cctacacgac gctcttccga tctnnnnnna gcattggctg agatccattg t 51
<210> 65
<211> 53
<212> DNA
<213> Artificial sequence
<220>
<223> rs6965933_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 65
cctacacgac gctcttccga tctnnnnnng ctgttatctg ttccaaccaa gga 53
<210> 66
<211> 52
<212> DNA
<213> Artificial sequence
<220>
<223> rs2712208_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 66
cctacacgac gctcttccga tctnnnnnng caggtcaaga caccaacaat ca 52
<210> 67
<211> 55
<212> DNA
<213> Artificial sequence
<220>
<223> rs12534598_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 67
cctacacgac gctcttccga tctnnnnnnt caacaactta aatgtacacg cacag 55
<210> 68
<211> 53
<212> DNA
<213> Artificial sequence
<220>
<223> rs28690459_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 68
tcagacgtgt gctcttccga tctnnnnnnt tgcctccagt tcactcctaa aag 53
<210> 69
<211> 53
<212> DNA
<213> Artificial sequence
<220>
<223> rs73421853_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 69
tcagacgtgt gctcttccga tctnnnnnng ggggaaaata gacaagaaga agg 53
<210> 70
<211> 57
<212> DNA
<213> Artificial sequence
<220>
<223> rs17154507_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 70
tcagacgtgt gctcttccga tctnnnnnna acatttatca gtagttccaa gccaact 57
<210> 71
<211> 49
<212> DNA
<213> Artificial sequence
<220>
<223> rs6944998_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 71
tcagacgtgt gctcttccga tctnnnnnnc ccacagatgc acccaagat 49
<210> 72
<211> 58
<212> DNA
<213> Artificial sequence
<220>
<223> rs10276013_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 72
tcagacgtgt gctcttccga tctnnnnnng gatggagtgt aatagtatgg gattatgg 58
<210> 73
<211> 52
<212> DNA
<213> Artificial sequence
<220>
<223> rs7805114_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 73
tcagacgtgt gctcttccga tctnnnnnng ctagtccatc aacttgcctt ca 52
<210> 74
<211> 52
<212> DNA
<213> Artificial sequence
<220>
<223> rs10242548_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 74
tcagacgtgt gctcttccga tctnnnnnnc aatgggtcgc attagtcaaa ga 52
<210> 75
<211> 51
<212> DNA
<213> Artificial sequence
<220>
<223> rs6949189_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 75
tcagacgtgt gctcttccga tctnnnnnnc ccttctccac cagactcaaa g 51
<210> 76
<211> 53
<212> DNA
<213> Artificial sequence
<220>
<223> rs1008707_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 76
tcagacgtgt gctcttccga tctnnnnnnt tgagcttgag cacattacca aaa 53
<210> 77
<211> 51
<212> DNA
<213> Artificial sequence
<220>
<223> rs78502829_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 77
tcagacgtgt gctcttccga tctnnnnnna gggtccaggc tggctactct a 51
<210> 78
<211> 55
<212> DNA
<213> Artificial sequence
<220>
<223> rs6466197_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 78
tcagacgtgt gctcttccga tctnnnnnnt gagtgttcag gttcaccaaa ttaca 55
<210> 79
<211> 51
<212> DNA
<213> Artificial sequence
<220>
<223> rs10487275_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 79
tcagacgtgt gctcttccga tctnnnnnng gggagctttt tagggagagg a 51
<210> 80
<211> 51
<212> DNA
<213> Artificial sequence
<220>
<223> rs3823958_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 80
tcagacgtgt gctcttccga tctnnnnnna aggggtggtc ttgctgacaa t 51
<210> 81
<211> 53
<212> DNA
<213> Artificial sequence
<220>
<223> rs6980029_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 81
tcagacgtgt gctcttccga tctnnnnnng cctttgttca gcttgtatcc atc 53
<210> 82
<211> 50
<212> DNA
<213> Artificial sequence
<220>
<223> rs4730261_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 82
tcagacgtgt gctcttccga tctnnnnnna ggcagtgctt gggtatgagg 50
<210> 83
<211> 52
<212> DNA
<213> Artificial sequence
<220>
<223> rs57592817_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 83
tcagacgtgt gctcttccga tctnnnnnnt ccctaacact cagcacagtt gc 52
<210> 84
<211> 54
<212> DNA
<213> Artificial sequence
<220>
<223> rs2253856_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 84
tcagacgtgt gctcttccga tctnnnnnnt catttttatc cactcctgca gtca 54
<210> 85
<211> 52
<212> DNA
<213> Artificial sequence
<220>
<223> rs982663_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 85
tcagacgtgt gctcttccga tctnnnnnnt cgttgtgtgg gaaatattct gg 52
<210> 86
<211> 51
<212> DNA
<213> Artificial sequence
<220>
<223> rs6965933_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 86
tcagacgtgt gctcttccga tctnnnnnna acacccccaa accctaagac a 51
<210> 87
<211> 52
<212> DNA
<213> Artificial sequence
<220>
<223> rs2712208_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 87
tcagacgtgt gctcttccga tctnnnnnnc cacatcctgg aatcatttcc tc 52
<210> 88
<211> 51
<212> DNA
<213> Artificial sequence
<220>
<223> rs12534598_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 88
tcagacgtgt gctcttccga tctnnnnnnt ttggggactg gtacgaaagg t 51
<210> 89
<211> 49
<212> DNA
<213> Artificial sequence
<220>
<223> chr13:20763486_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 89
cctacacgac gctcttccga tctnnnnnnc tacttcccca tctcccaca 49
<210> 90
<211> 49
<212> DNA
<213> Artificial sequence
<220>
<223> chr13:20763421_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 90
cctacacgac gctcttccga tctnnnnnnc tcccccttga tgaacttcc 49
<210> 91
<211> 49
<212> DNA
<213> Artificial sequence
<220>
<223> chr13:20763530_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 91
cctacacgac gctcttccga tctnnnnnnt gggagatggg gaagtagtg 49
<210> 92
<211> 53
<212> DNA
<213> Artificial sequence
<220>
<223> chr7:107323898_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 92
cctacacgac gctcttccga tctnnnnnnc aaggaattat taaaaccaat gga 53
<210> 93
<211> 51
<212> DNA
<213> Artificial sequence
<220>
<223> chr7:107350577_F
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 93
cctacacgac gctcttccga tctnnnnnnt gtgatagaaa agctggagca a 51
<210> 94
<211> 49
<212> DNA
<213> Artificial sequence
<220>
<223> chr13:20763486_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 94
tcagacgtgt gctcttccga tctnnnnnnc tacttcccca tctcccaca 49
<210> 95
<211> 48
<212> DNA
<213> Artificial sequence
<220>
<223> chr13:20763421_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 95
tcagacgtgt gctcttccga tctnnnnnng ctcctagtgg ccatgcac 48
<210> 96
<211> 48
<212> DNA
<213> Artificial sequence
<220>
<223> chr13:20763530_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 96
tcagacgtgt gctcttccga tctnnnnnna gatgagcagg ccgacttt 48
<210> 97
<211> 49
<212> DNA
<213> Artificial sequence
<220>
<223> chr7:107323898_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 97
tcagacgtgt gctcttccga tctnnnnnnt tttccaggtt ggctccata 49
<210> 98
<211> 55
<212> DNA
<213> Artificial sequence
<220>
<223> chr7:107350577_R
<220>
<221> misc_feature
<222> (24)..(29)
<223> n is a, c, g, or t
<400> 98
tcagacgtgt gctcttccga tctnnnnnng gttgaatatt taccgtttct aaaat 55
<210> 99
<211> 51
<212> DNA
<213> Artificial sequence
<220>
<223> Primer For
<400> 99
aatgatacgg cgaccaccga gatctacaca cactctttcc ctacacgacg c 51
<210> 100
<211> 55
<212> DNA
<213> Artificial sequence
<220>
<223> Index Primer Rev
<400> 100
caagcagaag acggcatacg agatctctta attgactgga gttcagacgt gtgct 55
Claims (7)
1. being used for the kit of Non-invasive detection GJB2 gene mutation, which is characterized in that
The mixture of 26 pairs of primers including the composition of primer shown in Seq ID No.1-46,89-91,94-96 is for specificity
Expand the different subgoal regions of GJB2 gene;To detect the variation of GJB2 gene simultaneously.
2. kit according to claim 2, which is characterized in that also comprising for being expanded to the pre- amplified production
Secondary amplimer, forward and reverse primer of the secondary amplimer is as shown in Seq ID No.99 and 100.
3. the preparation method of kit of any of claims 1 or 2, which is characterized in that including synthesizing and/or assembling Seq ID
The primer of nucleotide sequence shown in No.1-46,89-91,94-96.
4. preparation method according to claim 3, which is characterized in that also comprising synthesis and/or assembling for described pre-
The secondary amplimer that amplified production is expanded, forward and reverse primer of the secondary amplimer such as Seq ID No.99 and
Shown in 100.
5. a kind of method of Non-invasive detection GJB2 gene mutation, including steps are as follows:
(1) using subject's plasma dna as template;
(2) PCR is carried out to expand in advance;
(3) Index PCR bis- times amplifications are carried out to pre- amplified production;
(4) library Quality Control is carried out to bis- amplified production products of PCR, then carries out 150bp both-end in Illumina NextSeq
Sequencing;
(5) jointing in the sequence of sequencing is removed, is then spliced into a sequence and obtains primary template sequence, by original mould
Plate sequence is compared with human genome, by comparing the UMI molecular label sequence of primary template sequence, counts unique template
Arrangement set;Using unique template sequence, genome covering is calculated, for assessing library specificities;Sequence is mutated by calculating
The ration statistics of column and canonical sequence go out detection zone somatic cell gene mutation rate;
It is characterized in that:
The mixture of the 26 pairs of primers constituted in pre- amplification using primer shown in Seq ID No.1-46,89-91,94-96 is used
In the different subgoal regions of specific amplification GJB2 gene simultaneously;To detect GJB2 genetic mutation;
The region to be measured includes that 1 or several specific gene order regions, the subgoal region refer to a specific gene
In different zones DNA fragmentation;
A subgoal region in every a pair of primer amplification gene, the UMI molecular label sequence for including between primer pair is different,
For guaranteeing that the UMI molecular label sequence in the DNA cloning segment in same subgoal region is identical;And from difference
UMI molecular label sequence between the DNA cloning segment in subgoal region is differing from each other;
In the Index PCR bis- times amplifications, using forward and reverse primer shown in Seq ID No.99 and 100.
6. according to the method described in claim 5, it is characterized in that:It is pre- to PCR before carrying out the Index PCR amplification
Amplification gained DNA carries out magnetic beads for purifying.
7. method according to claim 5 or 6, it is characterised in that:After carrying out the Index PCR amplification, to expansion
Increase gained DNA and carries out magnetic beads for purifying.
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Citations (3)
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CN104131008A (en) * | 2014-07-24 | 2014-11-05 | 深圳华大基因医学有限公司 | DNA labels, PCR primers and application thereof |
CN105087756A (en) * | 2014-04-23 | 2015-11-25 | 北京贝瑞和康生物技术有限公司 | Method and kit for non-invasive measurement on fetus deaf pathogenic gene mutation |
WO2016176091A1 (en) * | 2015-04-28 | 2016-11-03 | Illumina, Inc. | Error suppression in sequenced dna fragments using redundant reads with unique molecular indices (umis) |
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2018
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CN105087756A (en) * | 2014-04-23 | 2015-11-25 | 北京贝瑞和康生物技术有限公司 | Method and kit for non-invasive measurement on fetus deaf pathogenic gene mutation |
CN104131008A (en) * | 2014-07-24 | 2014-11-05 | 深圳华大基因医学有限公司 | DNA labels, PCR primers and application thereof |
WO2016176091A1 (en) * | 2015-04-28 | 2016-11-03 | Illumina, Inc. | Error suppression in sequenced dna fragments using redundant reads with unique molecular indices (umis) |
Non-Patent Citations (1)
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YALAN LIU等: "A New Genetic Diagnostic for Enlarged Vestibular Aqueduct Based on Next-Generation Sequencing", 《PLOS ONE》 * |
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