CN108841823A - circRNA_26852及其应用 - Google Patents

circRNA_26852及其应用 Download PDF

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CN108841823A
CN108841823A CN201810588266.0A CN201810588266A CN108841823A CN 108841823 A CN108841823 A CN 108841823A CN 201810588266 A CN201810588266 A CN 201810588266A CN 108841823 A CN108841823 A CN 108841823A
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苗向阳
李嫒
解领丽
黄万龙
张秀秀
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Abstract

本发明涉及circRNA_26852及其应用，具体涉及circRNA_26852及其在猪分子辅助育种中的应用。本发明首次对大白猪和莱芜猪皮下脂肪组织中circRNA表达情况进行鉴定分析，以期能找出与成脂分化和脂质代谢相关的潜在circRNA。结果显示，circRNA_26852通过与ssc‑miR‑486、ssc‑miR‑874等miRNA结合，调控脂肪沉积和脂质代谢相关信号通路中相关基因的表达。揭示circRNA_26852通过与miRNA结合，进而调控信号通路中相关基因的表达，在脂肪沉积和脂质代谢中发挥潜在调控作用，为家畜的良种开发奠定基础。

Description

circRNA_26852及其应用
技术领域
本发明涉及生物技术领域，具体涉及circRNA_26852及其应用，更具体的涉及circRNA_26852及其在猪分子辅助育种中的应用。
背景技术
circRNA作为一种特殊的非编码RNA，普遍存在于动植物组织和细胞中，含量丰富，且具有稳定性，保守性和组织特异性，并成为了继miRNA、lncRNA之后RNA家族的一个新的研究热点。circRNA是一类由特殊选择性剪切产生的环形内源性分子，呈共价闭合的环形结构，没有5'和3'极性，不能被核糖核酸酶降解。研究已证实circRNA在疾病的发生和发展过程中具有重要的作用。有的circRNA在基因的表达调控中发挥重要作用，有的circRNA对RNA结合蛋白起调节作用，有的circRNA可作为翻译模板，有的circRNA可以通过顺式或反式作用影响亲本基因的表达。但是，circRNA是否参与脂肪沉积与代谢，仍不清楚。
大白猪，作为典型的瘦肉型猪，有较低的皮下脂肪含量。而莱芜具有猪肉色鲜红，肉质优良，持水性良好，脂肪含量可高达10.32％等特点。二者在脂肪沉积方面的显著差异为脂肪代谢的机制研究提供了良好模型。
本研究是首次对大白猪和莱芜猪皮下脂肪组织中circRNA表达情况进行鉴定分析，以期能找出与成脂分化和脂质代谢相关的潜在circRNA。结果显示，circRNA_26852通过与相应miRNA结合，调控脂肪沉积和脂质代谢相关信号通路中相关基因的表达。揭示circRNA可能通过与miRNA结合，进而调控信号通路中相关基因的表达，在脂肪沉积和脂质代谢中发挥潜在调控作用。
发明内容
本发明的目的在于提供一种与猪皮下脂肪相关的circRNA，命名为circRNA_26852，序列与SEQ ID NO.1具有90％以上序列同源性。
优选的，circRNA_26852序列为SEQ ID NO.1。
本发明的目的在于提供一种检测皮下脂肪的试剂，所述试剂检测样品circRNA_26852表达水平。
优选的，试剂采用测序技术、核酸杂交技术或核酸扩增技术检测样品circRNA_26852的表达水平。
本发明中的核酸杂交技术包括但不限于原位杂交(ISH)、微阵列和Southern或Northern印迹。
优选的，采用高通量测序技术、探针杂交技术、基因芯片技术或荧光定量PCR技术检测样品circRNA_26852的表达水平。
优选的，试剂含有与circRNA_26852杂交的探针或扩增circRNA_26852的特异性引物。
更优选的，引物序列为SEQ ID NO.2和SEQ ID NO.3。
优选的，样品为猪皮下脂肪组织。
所述核酸扩增技术选自聚合酶链式反应(PCR)、逆转录聚合酶链式反应(RT-PCR)、转录介导的扩增(TMA)、连接酶链式反应(LCR)、链置换扩增(SDA)和基于核酸序列的扩增(NASBA)。其中，PCR需要在扩增前将RNA逆转录成DNA(RT-PCR)，TMA和NASBA直接扩增RNA。
本发明的目的在于提供下述任意一项应用：
circRNA_26852在预测或辅助预测猪肉品质中的应用；
circRNA_26852在制备预测或辅助预测猪肉品质试剂中的应用；
circRNA_26852在选育具有不同肌肉品质猪中的应用。
上述试剂在预测或辅助预测猪肉品质中的应用；
上述试剂在制备预测或辅助预测猪肉品质试剂中的应用；
上述试剂在选育具有不同肌肉品质猪中的应用。
本发明的目的在于提供一种检测皮下脂肪的试剂，检测circRNA_26852相关基因的表达水平，相关基因为ssc-miR-486、ssc-miR-874、ABHD5、SMAD7和/或SCD。
优选的，试剂通过测序技术、核酸杂交技术、核酸扩增技术或免疫测定的方法检测circRNA_26852相关基因的表达水平。
进一步，所述试剂包含特异性扩增ssc-miR-486的引物；或特异性扩增ssc-miR-874的引物；或特异性扩增ABHD5的引物；或特异性扩增SMAD7的引物；或特异性扩增SCD的引物。
本发明的目的在于提供下述任意一项应用：
上述试剂在预测或辅助预测猪肉品质中的应用；
上述试剂在制备预测或辅助预测猪肉品质试剂中的应用；
上述试剂在选育具有不同肌肉品质猪中的应用。
一种检测猪皮下脂肪含量的方法，包括：(1)选取猪皮下脂肪组织；(2)检测样品中circRNA_26852和/或其相关基因的表达量。
优选的，相关基因为ssc-miR-486、ssc-miR-874、ABHD5、SMAD7和/或SCD。
定义：
术语“同源”是主要是指序列上的同源，也就是用来说明两个或多个RNA或DNA序列具有相同的祖先。同源的序列一般有相似的功能。一般来说，当相似程度高于50％时，常推测检测序列和目标序列可能是同源序列；当相似性程度低于20％时，就难以确定其是否具有同源性。
环状RNA(circular RNA，circRNA)，亦称环形RNA，是最近几年研究确认的一种新型的非编码RNA(noncoding RNA，ncRNA)分子。根据RNA构成的不同，环状RNA可分为三类：外显子环状RNA(exon circular RNA，ecircRNA)、内含子环状RNA(circular intronic RNAs,ciRNAs)和外显子-内含子circRNA(exon-intron circRNA,EIciRNA)。
“探针”指能与另一分子的特定序列或亚序列或其它部分结合的分子。除非另有指出，术语“探针”通常指能通过互补碱基配对与另一多核苷酸结合的多核苷酸探针。
所述探针具有与靶点基因的特定的碱基序列互补的碱基序列。这里，所谓“互补”，只要是杂交即可，可以不是完全互补。这些多核苷酸通常相对于该特定的碱基序列具有80％以上、优选90％以上、更优选95％以上、特别优选100％的同源性。这些探针可以是DNA，也可以是RNA，另外，可以为在其一部分或全部中核苷酸通过PNA(Polyamide nucleicacid，肽核酸)、LNA(注册商标，locked nucleic acid，Bridged Nucleic Acid，交联化核酸)、ENA(注册商标，2′-O,4′-C-Ethylene-bridged nucleic acids)、GNA(Glycerolnucleic acid，甘油核酸)、TNA(Threose nucleic acid，苏糖核酸)等人工核酸置换得到的多核苷酸。
本发明中的术语“杂交”用于指代互补核酸的配对。杂交和杂交强度受诸如以下的因素影响：核酸之间的互补程度、所涉及的条件的严格性、形成的杂交体的Tm和核酸内的G:C比率。
测序技术主要为高通量测序技术(High-throughput sequencing)，又称下一代测序技术(next generation sequencing)，一次对几十万到几百万条DNA分子进行序列测定，极大提高了测序效率。高通量测序平台的代表是罗氏公司(Roche)的454测序仪(RochGSFLX sequencer)，Illumina公司的Solexa基因组分析仪(Illumina Genome Analyzer)和ABI的SOLiD测序仪(ABI SOLiD sequencer)。
附图说明
图1是皮下脂肪差异表达circRNA分类图
图2是circRNA_26852-miRNA-mRNA互作网络图
图3是荧光定量验证候选基因差异表达图
具体实施方式
下面结合附图和实施例对本发明作进一步详细的说明。以下实施例仅用于说明本发明而不用于限制本发明的范围。
实施例1circRNA测序及差异表达circRNA挑选
1.1实验动物：
该试验以脂肪沉积存在明显差异的大白猪和莱芜猪为材料，饲养于莱芜市大千农牧有限公司，在相同饲养条件和环境下生长育肥，参照营养需要标准(National ResearchCouncil,NRC,1998)饲喂日粮，选择180日龄、种内体重接近的大白猪(约100kg)和莱芜猪(约35kg)各3只，且健康、体质优良。
1.2样品采集：
屠宰实验猪后，迅速将皮下脂肪组织分离。为减少RNA的降解，所有过程在冰上进行。之后用消毒剪刀将组织剪成小块，分别装入5mL冻存管，放入液氮冷冻，后转移至-80℃冰箱保存，用于提取总RNA。实验设置分为2组，分别对大白猪皮下脂肪组织(D_PX)与莱芜猪皮下脂肪组织(L_PX)中的circRNA进行鉴定及分析，每个样本设置3个生物学重复。
1.3样品总RNA的提取及质控：
取出等量低温保存的脂肪组织，使用QIAGEN抽提试剂盒提取RNA，提取的总RNA保存于-80℃冰箱。用NanoDrop 2000紫外分光光度计测定RNA样品的浓度和OD260nm/OD280nm比值，比值在1.9～2.1间，Bioanalyzer 2100检测总RNA的质量，且RIN≥7、28S/18S≥0.7，用RNase-free DNaseⅠ除去基因组DNA污染。
1.4 circRNA测序建库：
(1)Ribo-zero kit去除rRNA及RNA的片段化；(2)核糖核酸酶R去除线性RNA；(3)双链cDNA的合成与纯化；(4)末端修复，加A碱基；(5)测序接头连接；(6)DNA片段富集纯化；(7)文库的质检；(8)本试验共建立6个cDNA文库(大白猪和莱芜猪皮下脂肪组织cDNA文库)，分别为D_PX_1、D_PX_2、D_PX_3、L_PX_1、L_PX_2、L_PX_3，文库质检合格后，运用IlluminaHiSeqTM2500测序平台进行双端测序，对文库进行测序分析，下机得到的数据是原始测序数据(raw reads)。
1.5原始数据质控及过滤：
主要应用NGSQCToolkit进行质控并去除接头，后续的分析以clean reads为基础。具体步骤如下：
(1)首先过滤低质量的reads，质量阈值设置为20，过滤长度阈值设置为70％。
(2)然后从5’端及3’端去除低质量碱基，质量阈值设置为20。
(3)最后切除reads中含N部分序列，长度阈值设置为35bp。
表1原始数据质量控制结果
1.6 circRNA鉴定：
使用BWA软件与参考基因序列比对，生成SAM文件，对文件中的CIGAR值进行分析，并从SAM文件扫描PCC信号(paired chiastic clipping signals)。CIGAR值在junctionread的特征为xS/HyM或者xMyS/H，其中x,y代表碱基数目，M是mapping，S是soft clipping，H是hard clipping。关于双端Reads，CIRI算法考虑一对reads，其中一条mapping到circRNA上，另一条也需mapping到circRNA的区间内。关于单外显子成环，或“长外显子1-短外显子-长外显子2”成环，CIGAR值应该是xS/HyMzS/H以及(x+y)S/HzM或者xM(y+z)S/H，CIRI软件能够将这两种情况分开。关于splicing信号(GT，AG)CIRI会考虑其它弱splicing信息例如(AT-AC)。算法：从GTF/GFF文件中抽取外显子边界位置，用已知边界来过滤假阳性。
1.7 circRNA注释：
将circRNA与基因元件进行比较，获取circRNA在基因组上的位置信息。利用circRNA位置信息与已知数据库中的蛋白编码基因注释信息，对circRNA进行注释，获取序列信息。
1.8 circRNA分布统计：
统计各个组织中circRNA的数目情况。统计预测到的所有circRNA长度分布情况，在基因组染色体上的分布情况，circRNA外显子数目情况。
1.9 circRNA表达水平定量：
利用RPM(spliced reads per millon reads)对circRNA进行定量，其中RPM计算公式如下：RPM＝number of circular reads/number of total reads(units inmillion)其中：number of circular reads表示比对到circRNA的back-splicedjunctions区域的reads数，该数值来源于circRNA预测软件中提供的支持circRNA成环的reads数目；number of total reads(units in million)表示每个样本测序数据中(clean_data)reads数目(单位为millon)。
1.10生物学重复样品间相关性分析：
样品间circRNA表达水平相关性可以用来检验实验可靠性和样本选择合理性。相关系数越接近于1，说明样品间表达模式相似度则越高。当样本数目较多时(≥3)，circRNA的表达量情况，采用R语言中的cor函数及corrplot包，计算样本间的pearson相关系数，并绘制热图进行展示。
1.11样品组间差异circRNA筛选：
由于实验有生物学重复，运用DESeq包(Huber&Anders,2014)，是基于reads count进行差异分析的R包，用负二项分布检验的方式对reads数进行差异的显著性检验，估算转录本表达量的方式采用basemean值来估算表达量，计算差异倍数。筛选差异的条件为p<0.05且|FoldChange|>2。
在莱芜猪和大白猪皮下脂肪组织(L_PX vs D_PX)中共鉴定得到29763个circRNAs，对其进行差异表达分析(按照|FoldChange|≥2.0和P-value<0.05严格筛选)，在皮下脂肪组织中共鉴定得到275个差异表达circRNAs(70个上调，205个下调)。在70个上调的circRNA中，有17个intergenic circRNA，3个exonic circRNA，49个sense-overlappingcircRNA和1个antisense circRNA；在205个下调的circRNA中，有7个exonic circRNA，21个intergenic circRNA，174个sense-overlapping circRNA和3个antisense circRNA(见图1)。其中，sense-overlapping的circRNA_26852进入我们的后续研究范围。
实施例2 circRNA深度分析
2.1差异表达circRNA功能分析：
得到差异表达circRNA之后，利用circRNA来源基因的信息，我们对差异表达circRNA进行GO富集分析。统计每个GO条目中所包括的差异circRNA个数，并用超几何分布检验方法计算每个GO条目中差异circRNA富集的显著性。利用KEGG数据库对差异circRNA来源基因进行Pathway分析，可以找到富集差异circRNA来源基因的Pathway条目，寻找潜在的不同样品的差异circRNA可能和哪些细胞通路的改变有关。
2.2 circRNA-miRNA-mRNA互作网络图
选择在莱芜皮下脂肪组织下调的circRNA_26852进行后续的分析，结合生物学信息学分析和本实验室前期做过的大白猪和莱芜猪皮下脂肪组织miRNA和mRNA的测序结果进行整合分析，挑选出了与circRNA_26852结合的ssc-miR-486、ssc-miR-874，且这些miRNA在大白猪和莱芜猪皮下脂肪组织差异表达，进一步分析ssc-miR-486、ssc-miR-874调控的靶基因及其表达情况，将分析的数据进行整理，构建了circRNA_26852-miRNA-mRNA互作网络图(见图2)。图中的其它miRNA也是circRNA_26852的潜在靶向，我们对图中miRNA调控的一些靶基因也进行了qRT-PCR验证，结果见实施例3。
2.3 circRNA_26852靶基因GO和KEGG富集分析
为了深入探索circRNA_26852靶基因的功能，对靶基因进行GO和KEGG pathway富集分析，结果显示，circRNA_26852通过与相应miRNA结合，调控脂肪沉积和脂质代谢相关信号通路中相关基因的表达，circRNA_26852靶基因富集于甘油三酯分解代谢过程、脂质贮存的负调控和磷脂酸生物合成的过程等与脂肪沉积和脂质代谢相关的生物学过程。
实施例3实时荧光定量PCR验证
选择circRNA_26852，circRNA_26852靶基因中对的2个miRNA(ssc-miR-486、ssc-miR-874)和相关基因SCD、SMAD7、ABHD5，每个设置20个生物学重复(大白猪和莱芜猪各20例)，分别以甘油醛-3-磷酸脱氢酶(glyceraldehyde-3-phosphate dehydrogenase,GAPDH)基因和5S rRNA为内参，qRT-PCR方法用于验证基因的表达水平。
3.1引物设计：
circRNA_26852扩增：
正向引物：5’-CACCCTGTGAGAGCCAGATTTA-3’(SEQ ID NO.2)
反向引物：5’-CTGAAGCGCTGTAGTACCTGG-3’(SEQ ID NO.3)
SCD扩增：
正向引物：5’-CCTTTATTCTTCTCTAACCCGT-3’(SEQ ID NO.4)
反向引物：5’-TCAGTGAGCAGAGACTTGTG-3’(SEQ ID NO.5)
ABHD5扩增：
正向引物：5’-GAGGAGATGGACTCCACG-3’(SEQ ID NO.6)
反向引物：5’-GGGAAGCCAACCAGTTAG-3’(SEQ ID NO.7)
SMAD7扩增：
正向引物：5’-CAGCTCTACAAGAAGCCGA-3’(SEQ ID NO.8)
反向引物：5’-AAGAGAAAGGACGATGGCA-3’(SEQ ID NO.9)
ssc-miR-486扩增：
特异引物：5’-TCCTGTACTGAGCTGCCCCGAGTT-3’(SEQ ID NO.10)
ssc-miR-874扩增：
特异引物：5’-CTGCCCTGGCCCGAGGGACCGAC-3’(SEQ ID NO.11)
3.2反转录体系：
将下列物质加入0.5ml的离心管中：RNA 5μl；Oligo(dT)2μl；dd H2O(DEPC处理)4.5μl；
以上首先70℃孵育5min，然后迅速放在冰上。
5×Buffer 5μl；dNTP(10mM)2μl；Ribonuclease inhibitor 0.5μl；M-MLV RT 1μl；以上20μl体系42℃孵育60min，然后70℃10min。
3.3 Real-time RCR反应
(1)将所得的cDNA与下列物质配成20μl体系加入0.2ml的离心管中混匀。
Real-Time PCR体系：cDNA 2μl；引物1(10pmol/μl)0.5μl；引物2(10pmol/μl)0.5μl；SYBR mix 10μl；dd H2O 7μl。
(2)Real-Time PCR程序设置：94℃预变性10min；94℃15s，60℃60s，45个循环；72℃10min。
miRNA扩增体系及步骤参考miScriptGreen PCR Kit说明书。
3.4结果统计分析：
为了验证测序分析结果，选取目标分子circRNA_26852、ssc-miR-486、ssc-miR-874、SCD、SMAD7、ABHD5，进行验证。2-△△Ct法分析每组样本间基因的相对表达情况，用t-检验统计分析相对表达量，数据结果展示为平均数±标准差(Mean±SD)，P<0.05为差异显著。
结果显示，circRNA_26852、ssc-miR-486、ssc-miR-874、SCD在瘦肉型猪大白猪皮下脂肪组织显著下调表达，SMAD7、ABHD5在瘦肉型猪大白猪皮下脂肪组织显著上调表达，具体结果见图3。荧光定量PCR结果与测序结果一致。显示circRNA_26852、ssc-miR-486、ssc-miR-874、SCD、SMAD7、ABHD5可以作为猪皮下脂肪含量检测的分子标志物，在预测或辅助预测猪肉品质、选育具有不同肌肉品质猪等领域具有很好的应用前景。
序列表
<110> 中国农业科学院北京畜牧兽医研究所
<120> circRNA_26852及其应用
<160> 11
<170> SIPOSequenceListing 1.0
<210> 1
<211> 74721
<212> DNA
<213> Sus scrofa
<400> 1
ccagatttac tgccacgggg agctcctgcg ccaggttcag atggccaggc tctaccagga 60
tgataagcaa tttgtggata tgccattgtc cgtagctcca gaccaggtac tacagcgctt 120
cagcgagctg gcccaggccc acaacttcag catcccccag caggagctgc aggatttcat 180
ccgagaacac ttccaggctg tggggcagga gctgcagccc tggacccccg aggactggaa 240
agacagcccc cagtttctgc agaagatctt ggaccccaag ctgcgagcct gggcagggca 300
gctgcaccag ctgtggaaga agctgggaaa gaaggtgaag ccagaggtcc tcagccaccc 360
agagcggttc tctctcatct actcgggtca cccgttcatc gtgccaggag gacgctttgt 420
cgagttctat tactgggact cctactgggt gatggagggt ctgctcctgt ccgagatgcc 480
agggacggtg aagggcatgc tgcagaactt cctggacctg gtgcagacgt acggacatgt 540
ccccaacgga gcccgcgtgt actacctgca gcggagccag cccccgctcc tgagcctcat 600
gatggagcgc tacgtgactc aggccaatga caccgccttc ctccgggaca acctggagac 660
cctggccttg gagttggact tttggaccaa gaacaggagc atctctgtga gctctggggg 720
aaagagctac gtcctgaacc gctatcatgt cccctatggg ggacccaggc cagagtcata 780
cagcaaagac gcagagctgg cagccacctt gtctgaaggg gaccacgagg ctctgtgggc 840
tgagctcaag gcaggggccg agtctggctg ggacttctct tcacgctggt tcgtgggagg 900
cccgaaccct gattcgctca gcagcatccg aaccagcaaa ctggtgccgg ttgatctcaa 960
tgcctcaggc aatgactccc aagctgagaa gtacagaaat ttgcgagcac agcgcatggc 1020
cgccatgaag gacatcctgt gggatgagga gaaaggtgcc tggtttgact atgaccttga 1080
gaacggaaag aagaaccttg agttttaccc atccaacctc gctccgctct gggctggctg 1140
cttctctgac ccaggcgacg tggacaaggc tctgaaatac ctggaggaca gccagatcct 1200
cacctaccac tacggaatcc cgacctctct ccggaagaca ggccagcagt gggacttccc 1260
caacgcctgg gcccccctgc aggacctggt catcagaggt ctggccaagt ctccttcagc 1320
cagagcccag gaagtggctt tccagctggc ccagaattgg atccgcacca actttgatgt 1380
ctactccagg aggtcagcca tgtacgagaa gtatgacatc agcaacggtg gacagccggg 1440
tggtggaggg gagtatgaag ttcaggaggg gtttggctgg accaatggag tggtcctgat 1500
gctcctggac cgctatggtg accggctgag ctcagggacc cagacagttt tcctggagcc 1560
ccactgtctc accgctgccc ttctgcttgg cctcatgctc agcctcctgc cacagtgacg 1620
ggccttctca tctggctcca gctcctgcca attaaacctc ggcatgagtc cctaccttct 1680
cctgcctctg gatcctttat ccctggagag cctgccttcc gccccagccc agccctcagt 1740
tatattggag taggagccag gcagggacct ggaggtcatg gtgggggagt taacatggtg 1800
ggggagttcc cgtcatggcg cagtggttaa cgaatccgac taggaaccac aaggttgcgg 1860
gttcgattcc tggccttgct cagtgggtta aggatccggc gttgccgtga gctgtggtgt 1920
aggtcgcaga tgtggctcag atctggcgtt gctgtggctc tagcataggc cagtggctac 1980
agctctgatt agatccctag cctgggaact gccatatgcc acgggagcgg ccctagaaaa 2040
ggcaaaaaaa gacacacaca aaaagtggga atcctgcttt gtgggaacaa cctagcagcc 2100
tacaccacat accccagccc catgcccccc ataacatggc ctcccaaaca catagtccaa 2160
atgctttatt gttctgctga gatgcttaca aatactgaaa aacacccagc ctggacccag 2220
caaccatggc ccaggaccag gaagagggac agggaggtgg gcttagtgtt aaggcgctaa 2280
gggctgagcc cagacagctg gaggcctggt cctctgctct ccgtttcatc cctcttctga 2340
ggccacagga agggcaacca gatctcaagg ccccctctgc tgcttcctct cctgctcagg 2400
gcttccctca gggagcatct gagcaaccct ctgcccatca gaagctcagc cccataacaa 2460
accccgagga cagcccttcc agcttcctct ctcagccagt ggctcctcct ctggtctcta 2520
gttcccggct tcgtcccttc tggctctaag caaggcacga gtggaaggga gagggttgga 2580
ctggaggccg gcaggctaca tatcagggcc tggagacaca ggcttggggg ggagaaggtg 2640
ttcagtcccg tccccccagg tggagatgcc caaggacctg ggctggagag ataatggcac 2700
gtaccctcag ccgggcccac agttgaagca gcaggctgag ccacagagcc acggcaatta 2760
gtatgtaacc atggcaatga agcagtcact acggtgacca tggtgctggg tctgcaaaaa 2820
ggggagaggc tgggacctga aggatgacac ccgtggtctc tggcccttgg caaagtaatc 2880
aacatgggtc aacaacgggc tagagcaggg tttgagaaga acaccgcctg agctgttacc 2940
ccactctgga tgtggctctc tcagccccca aggcccctgc agagggggct gaggcaagag 3000
gggatctttg gcagattctg gtactataag ttgtccttaa caaaagagaa cacaggctgg 3060
ggagcgcagg tgccaggtca gggcagaggg gcccccctgt gacagctcct tgcctgtctg 3120
ggcccggggc aggaccctga agggttattg cgagttctgg gctccaggag gcaacaaggc 3180
cccctcccca acttttatct cctggctggt tctttagagg gccagagctc agaaactcac 3240
aggaagagct ccttacagaa tgttccagag tctcttctgg cacaaaagcc ccagtatgct 3300
ctgcccttca gggcctctgg cagcattcct gtgaggccca aggcccaggg tgccactctg 3360
gctccagggg cgagcagagc gcaggcagtt ggcagggtcc tggggcccgc ccaaggggcc 3420
agaggcccat tccgtcagtt catgaactga gtgtagccct cagccccggt gacgatgacg 3480
tccatccaga tgcgcatggc ctccgcggat ggggccacca tgtagtacag ccggtcgtgg 3540
gtcttcacac agaaggtgag ggctgggttc gggctctgcc gagggggaga ggaaggtcta 3600
agccgcggcc cacccccgag gcccaggagc acctgcctgg agcttggagc cgagtcctaa 3660
ctaggcctgg agcccagtcc ccaccatcaa gtgggcccca agctgagatg ctcccctggg 3720
cagtccttgg gccctggcag ggccagaccc cgccagatgg gggcaatgtt ccctggcatc 3780
cccaggcaca aaagggaata tcaaaagaat cagacttgga gttcccactg tggcaaaagg 3840
ggatcggcgg catcttggga acgcagggat gcaggttcga tccctggcca ggcacagtgg 3900
gttaaggatc tggtgttgcc acacctgtgg cttaagttga aactgaggct cacatccgat 3960
ccctggatcc ctggcctggg aactccgtgt acctcagggg tggccaaaaa atgaaaaaac 4020
aaacaaacaa aaaaagaatc agactcagga gttcctgttg tggctcagcg gtaacaaacc 4080
caacttgtat tcatgaggat gagggttcaa ttcctggcct cattcagtgg gataaagcat 4140
ccggtgttgc cgtgagctgt gctgcaggtc acaggcatcg ctcagatccc gcattgctgt 4200
ggctgtggcc taggcctata gctgtagctc caattcaaac cctagcctgc ccttccatac 4260
gctgcaggtg cagccctaaa agaccaaaaa aaaaaagaat tactcaaaga gctgttccag 4320
caagggccag aggctcttct cagcaaaaga ggaacagatg gggaaagctg ggatgctggg 4380
ctccagggcc ccagaggagc agagatgagg gctggagcgg gcgtctgagg cttggcggga 4440
gagaggccga cttgcatgaa ggcagccggc cgcagcctca aacctctcct gttgatcaat 4500
cctctgcacc aagtctaatc tctgaggccc cccactccct tctgcatccc ttctgcgctg 4560
cgccagagac ctcctgccgc catcctagga gagggaggca agcaagccca ctggaaccca 4620
caggggcttc ctgctctgca acccacctgg ctcctccctc aggcccgggg ggaaggggct 4680
taggggagag tcaagggtca ggggtgagag ggcataggag gcgttaatgt gcggtagttc 4740
tagttacagg tacgtgggga ggggtacctc agtcaccaga gtgaagctga aaaacctctt 4800
ctgggaaagg gagagggaga gaggaagaga atgtttacaa agggcacaag gaacgggtct 4860
gaggccagaa aagagcgggc tgggcccacc ccttcccctt ccccttcccc ttccccgggc 4920
tgcccctctg gccctcacct tggctgcact gcgcaggtgg tcatagtaca cttcctcgat 4980
ggcctggaaa tagatgaccc ccttcagctt cgtctcatgc ttgtctgcaa aggaataagg 5040
gccgggctga ggaggcctgt cacacttccg gtcaggcgcc atggacctgg agcgtcccca 5100
tccaggaaac agggtgattc ccaggcctcc acagcagagc ccagcaggga gccccaagga 5160
ccgagttggt gctcagcttc cctcagaatg acagaggctg cgaatcagaa ctccacccag 5220
acaccacttc ccacttatcg caatggcaag aatataccca aaatacacag catacttttg 5280
gcaagtctgt ggggaaatag gtagaatgat aaacccgaga ggtgggagta tagttggtat 5340
ttatgaacga tttggccata tcctccaaag ttagaaaggc atttgccctt tgagccaatg 5400
tttacactga tgaaaattca tcttacaatg tacaaaataa caaatttaca agataagtca 5460
ccccagcagt atttacagtc acaaaagact ggaaacaacc cacgtatcca gcaagtggcg 5520
atggctaagt aaattagact gcaccactcg gcagaacaca cacagtgctt gaacagaatg 5580
acagagaaat atctgatgca gattcagaag taaatctata aaatacatcc agatttaaat 5640
atctagatag acctatgtct gagacagaac tctaagttac attagcagaa caacaaagat 5700
tgtatcgtgt gctactttct gtgtgagaaa caggttaaga atatatgttt gcatttgttt 5760
atattcccta aagagactct agaaggatac acaagagatt aacaaaagaa gttatctcta 5820
gtgagacagg gcataattct caatttaaac ctttttctgt gtttgaagtt cttagcatgt 5880
gaatgcattt atctgctcaa caccaacact caaaaatagg agcataaggc atcaggaggg 5940
aagggtggca ggaaggaggt gggcagacag agcagatgcc cgctccagtg ggggcagagc 6000
actggcagca aaagcacagg gcctggccca ccccctcgtg tgcagacact gagcaggcac 6060
ccaacagaca gcctggacag caagcctgag tcacacagaa aaggtcacac ggtcaccaca 6120
cgccgatgac caaggctgac acaggcacct tctcccaccc acctcctggg ctggttcagc 6180
ctgaatcact gaccctggat catcactaca gcctcctggt gtgccctgcc ccacctctgc 6240
cccaaacccg ccttccctca ggcccagggg ctgagctttc tgaagcaaat tgaatcctgc 6300
tgcctcagct cacagcgtct ctggggttcc ttcactggaa gctgctgcct ccctggttat 6360
tgacaaaggt tactggcaaa ggctactggc agttcggtgc caacacgtcc ctctggcctt 6420
gcctcatgtc cctcccctgc accagcccca cgtgctatct ggccaggctg agcttctcac 6480
ggtcgcttaa cagcccactc tgccaacact ggaatggtct ccagcccaat cccttggcaa 6540
actcctacgt catttccaaa accctttgca aatgtcacca ctagtgcaca gctgtgtctc 6600
ctatgctttc acaatacatg ttcctctgta cagaacttat tctgctgaat ctgaatgctc 6660
ttttatctgt tcacaggtct agctcctcca acacggactg cgtgcctagc aaaagggaag 6720
tgttcagtca atgttgaata aatgagtcag aggcactggg atccaaagaa aagtaagaga 6780
cctttctctc tctctctctc tttttttttt ttcttttctt ttttgtctac acctgaggca 6840
tatggaagtt tccagaccat ggaatgaatc taagctaaga ctgcaacctg tgtcacagct 6900
ggggcaatgc tggatcctta cctcactgcg cccggctagg gatcaaacct gcactgctag 6960
agacaatgcc agatccttaa cccactgtac cacagtagga actcagagac ccttctctta 7020
ggaacccaca acctagcagg gaagaaagca caggcacaca gcccttcatc agctgtctga 7080
cttgggcatg gttaggattc caagagaaca cctgcaggtc gcaggtggtc ctgaatatga 7140
agggacaaaa cacactccaa gggctcagca ccgtgtctgg cacaacacat gtggcatctg 7200
tcatgacatc aatatgcccc agggctgatg tcacatggca gatggagcct gtgtaacaca 7260
ggagttatac ctgagggcag gaatcgtttc cagtgagctg gggccaggaa aggcttccca 7320
gaggtggatt ctgatctgga ccctgcagga tggaggggtt tcacggggta ggtgggaaga 7380
cagtgcaggc aagggcaggg agaatggacg agctgaggag caggcaaggc acagccaggg 7440
tcctgaccca gccacaggcc catctccctt ggacacttcc tcctcgggac agcgacccag 7500
ccccatcccg gcccctggcc cggccatccc tgaagcctcc attgtctccc atctcttcct 7560
ttcctggaaa tagtaacaca ttcctgcctc tctgtttcct taccacccac ctaccctcga 7620
ccttgtgcaa tctggctccc accccagtac gcagtggaga cgattctctc atcaagctcc 7680
aatccctgaa tcaaacagcc tttgggtcca cattactcgt cgcctttctg cagcacccag 7740
catgattgtg acggcccccg cttgcacaca tctactcctt tggttagctt ccttctcagt 7800
ttctgccccg ccctcccctt cccctgacca ggtctccttt tgtttcatag acattaagga 7860
aggtcgcata tgccaagtgc cagcccagca cctacatgtg gcttcctgcc tcttgtcctt 7920
ggctggtgcc tccttttcct ccttccccac cagggagggc ctgccccagg gtgcagctct 7980
gtctctccct gagttgccca agttcagtct tagccctgct gcttggccgc tgagacccct 8040
tgggcaagtt tcctaacatc tctgagccta attttcctca tctgtaaaat gggtgtaaaa 8100
atagtgcttc cctcacaagg ttattatgaa aataaacgag acaatgcatg tagagggtag 8160
agccctgcag ctggttcatc caagggtcca gtatgatctg ctgtcgtcat tgtcatcatt 8220
attataccaa cagcttcggc ttgcactctg cctccaaccc tgacctttct ctctctctaa 8280
ctcttttttt tttttttggc ccccacccac agccaaaagt tcccaggcta ggggtcaaat 8340
gggacatgca gctgctggcc tacaccacag ccacatgcca gatctaagcc tcgtctgtga 8400
actacaccac agctcacagc aacgccggat ccttaaccca ccaagcaggg ccagggatca 8460
aacccacatc ctcatggata ctacttgggt tgctaccact gagtcacaat gggaactcca 8520
ccctgacctg tctcttactt agatacccca ggggctcttc aactcaacag gcctaaaatg 8580
gagttcttct cccccagcct gttcctctca atcctgacaa agtctctttc cagcccaggt 8640
ggaaacatgg gaacacatgt gccttggcct ccccacacca agggcccaga tagttaggga 8700
gggcttcttg gaagggatgg cacttggttg gagaccaggt caggggcaag tagggtgctc 8760
caggcctgca caactccagc accaaacact ctggcccttt ccttccaggc tctgagcaag 8820
atcgctcttc ctggatttgt ggttggctgg ggctgggtga ctggctgaac agtcccactg 8880
gtgagttggg gacagactcc agcagtggca tttaacgcca ctgaaaaacc aatcaggctt 8940
ctctttatcc tcggccacca caaccagcaa tgttccagaa agacatgctc tgaagcaaaa 9000
agaaagctga cgaggaacac aggccccacc aacccacagt gtacatggac acctatcggg 9060
agtaagaagg aaaagtttgg ttttgagcca ctgggatttt agggtgggtt gattcctcag 9120
cagagcctag cctatcctga cctgcacaca catagcagct cacgctccca gcaagaactg 9180
tccaaaggga gttccaggga tgggaactaa gttggagccc aggtggagag agctgaggga 9240
gctctcccca gggggcctgt ccccagggct gtcctgagcg tccctgggca ctgctcagca 9300
gcttgccttg gggtcttcac aggaactcac ccaaggggga atccatagga ccccaggcac 9360
ccacagcatc tgggtgtccc ctacagcatc tccaatattc ttgtccagta tctgcctccc 9420
tgacaacagg ctccaaggga gagagctgtg gccactttgc ttattaccag cacccagaac 9480
agtgcctgga acttattagc tgctcagtaa tactgctgaa taaacgaatg aatgaacgaa 9540
caaatggagg ggaaccaggt acaccctgta cattttcaga gagaggggat caagcccaag 9600
cccctggccc ccacttggaa gcctggcctc cttccagaag acccaacatc ctgggtgaca 9660
gagtcccagg ttccctgtgg aaacttaccc acataatagg agagggtgcg cttgagccga 9720
tcgaagacaa accagcgctt cttccatgat ttaatcttgc cgcccatctt gaccaagtag 9780
ccacggcaga cctggtagga agtgaaagtg aggaaggacc caaatgcaca gcccccaaaa 9840
ctttcttccc tcccttttct tttctttctt ttttcttttt ctttttttct ttttttgctt 9900
ttgagagcca cacctccagc atatggaagt tcccaggcta ggggtgaaat tggaactaca 9960
gctgccagcc tgcaccacag ccagggcaat gccagatcca agccgtctgt gacctacacc 10020
tatagctcac ggcaactctg gatccttaac ccactgagcg aggccaggga ttgaacccgc 10080
agcctcctgg atactcgtca gattcatttc cactgtgcca caacgggaac tctgccctaa 10140
acttttcttc cctgagcggc gatgtcagcg ccatctcctg tgcctatagt catggggggg 10200
gctgtcccca acatctcttg cggccagcac acctggtcct gggacagaga cctcaggggt 10260
tagggcagga actgaggcca ctattcaaag gactcaggag gtgcccctgc caggggggcc 10320
ccaccccctg cccctgcccc tgtaccttgc tgctgagcac cacgtgcagg cacgtatcca 10380
caccgtggcc tgacgactca atgtgggtct tcaggtcaaa gtcctccttc ctgattggca 10440
ggtagcgggt caggggtcgt gcctgggagc agagggaggg tcaatagagg ctcctctctt 10500
ccccaccatt cccttgtcca gacctcttgg cttgggtccc gaagcccccc ccaccgcagc 10560
ctgccatcac cccctccccg cccccgcagc tgacaccccg gtttcttggg gcttcggtaa 10620
aagggcctgg ccggcctgtg ctgactccac cgaggcctgg gtaaactgcg aatggttcta 10680
cgcctcagtc tccccaccta agatccaaaa atgtgggcct tgtcttgtcc ttgcccctcc 10740
tcaccctccc gcacagaatg gaacaaagca gacaaccaga gaggaacacg ctcttggaaa 10800
aaaagtccga taagaagcct gggctctgac ttcccgtggg gtcttgtttg ggtcacgaca 10860
cctctctggg cctctgtctt ctcctacata aacgagcact ctgaactaga ggatctctgc 10920
ggtctggtct atctcaagat ttctgatact aaactcgaat tgtcagtcca ctgcaatttt 10980
ttcctactct tcctttctaa gaaaaaacat tagcatgaca tgagaagaaa acatcacaaa 11040
ttatttaaag aaaaaaagaa aacgctcaac ttcccagagg ggccccaaag atgagctgac 11100
tccacccacc tccccaaagc tggtgggacc tgaagccacg cctgagcccc agggcagaga 11160
gctagagagg gtcccccagt gaccactgac agagacagga aagaggaact aggcatctga 11220
caaagattct aagcacagag gtaaaactca gagtatttct tttatttcta aattcccaaa 11280
gcaatctaag caacctaccc cgtagaattt acattgctgc atcagtagaa aatcaggggg 11340
ctgtgattgc cggtcactgc tgtgtggagc ccaccctggg gaggcctaag gagaccccat 11400
aggaaagggt ggcctgtgag tctcacgggc tctcccaggg gcccaggatc tgggaccaga 11460
gacacaggta gatttcccta cacaacctaa tttctctcta ccttcaactg ctctgctcaa 11520
gtctcctgta taaggaaaaa gcccaggctg agtttttgtg taaggatgga ttctggtccc 11580
taggagaaat tggtacaatt ctcttctgga gacctaaact gaaagtcaga gtgaggccag 11640
ctgctggagg tggagctgca gccaaaaggt ctggtggaac agggccagga cagccacgaa 11700
gcccaggctc ggggcaggga agcagaaagg aggggcaggg cagactcatg agacagaggt 11760
gaggccctcg acagaggcca agggagcccc cctgattctc gactcagctc ccacctctag 11820
agggcctgtc cagaccctct tctctgaccc cagtgtctac aaggtgtcca gccccgtcct 11880
cacaactcac ccactcccaa tacacacata cacacactgg cttgagcaag ccctggtggg 11940
tccctcttcc ttggggccaa acaaatccta ggcagagctc tgctcgtgca accccttctg 12000
ggcagaacag gcttgatggc ccagcagggg tgccctggga taaccaaccc ctgcacagct 12060
ccactaccca ctcacctgtg aaaactgttt ctcccgcatc ttgacctcct tctccaccag 12120
ctgctgcctc cgggcactct cgctctgcag cctccgttcc acctgctccc gccgcctccg 12180
ctcctcctcc agagcctgcc gcctcagctc catctcccgc tcctgccccc aaaggcagga 12240
ctcagtgccc atagcctccc tcttctcccc cgaccccctc catccctcct caggctgggg 12300
cagagctgca ggaggcaccc acttccgagg gcggcctggc atgaggggcc accatcctcc 12360
aagcccatcc tctccaagga cctcgctgac aagggtaaat gccttaaagt gacacctgtc 12420
tggctccaaa ccctggctct gccaccacta ggagctctag accctaacag aacgatttaa 12480
actctctaag cctcagctgc ctcatctgta aaatggggcc aagaatgcct actcctggga 12540
ttatagcgca aattaaatga gatcatagaa ataaacgccc atcactgaca ctggctggaa 12600
ggaggcaccc aatagacaag cggagctttt ataatttttt ttccttcttt tttttttttt 12660
tttttttttt tgtcttttta ggaccgcacc tacagcaaat ggaagttccc aggctagggg 12720
tcaaatcaga gctgtagctg ccagcgtaca ctacagccac agcaacacgg aatctgagcc 12780
ttgtctgcaa cctacaccac agctcatggc aatgctggga tccttaatcc actaagcagg 12840
gtcagggatt gaacctgtgt cctcatggat actagtcaga ttcgtttccg ctgagccctg 12900
acgggaatgc cggggctttt ataactttta ccttaatcca ccataacaac cctcatatct 12960
ttaactcctg ccatttcttc ccagcccaaa acttagcaga atctcctttt tctccatttg 13020
ccaagagagg tttatcaatc tgccctcctt tgaagtgagt gagactaaag ggccttcttt 13080
cctggttggt ctcctgctcc ccggggccca ttctgatctc taaacaagga gggtgcctgg 13140
atggtagaga gaacagcttc tgcagagtct attaaacctc ctcccggtgc gggttcctca 13200
gccaggctcc acccactgta aacaggcccc tctgctgagc cccagcccca gcccgcactc 13260
tgcaccagcc tggacagctg ccctctctcg gttccatcct tcaatcccag ttcctaacca 13320
ttcccaagag gaaccacctg ccactcaggt agagagggat caaaattccc atggtggcca 13380
aacgtccaca gaagccaaca aacaaagact ggacgttccc acacgggaag gtcatggttg 13440
gacgatggtc actctgctgc ctcctcttcc taagagctgt gaacctgtaa tctcgccaag 13500
cctccctctc cccgacttga ggaagacagg cttttactac attttcctcc tcaagggacc 13560
actgtgagga caatgtgggt gaaggggctt ggggaggagc agaggccctc ggcaggcgga 13620
gggcagcccg gggccgaggg accttctccc cggtggccca gatctgcctc acccttgact 13680
ccatgagccg gctcttctcc gcgtgagctt cttttagcat cttctccatt tcctcgatct 13740
tccccacatc caggccgctg gtactgggga aggaagggga ggaagcacca gtggaggcgg 13800
tggccctggg gtggggagga ccgaggcggc gccatgcgtc aggcggcagc cgggctgggg 13860
tacctggaca tgttgtcagg ggagcaggcc gagttgcccc ccgtagagat gctggtctcc 13920
atgctgtccg agctctccag gctcagcgtg tcgtaggcat gctcgccctc ctccccgcgc 13980
tcccgcgcag ccggcaggtg gtgcaggatg gagtggtgtg cgagcggtgg gaagcccgag 14040
ggcccggtgg ggaagggggc cgccccgtgc agggcatccc actggcactg cgcctctgcc 14100
gctgccttct gcttcagctc cgccagccgc cgacgctgct cctcaatcac ctgctgccct 14160
gcaggaggga caggtgggag tcacgctggg caccccaccc actcagggag gacactggca 14220
gcatcacaga tggagggagg aggcgagcag gggagaagat aacagggcag ccccctcgcc 14280
cccaccccca ccagcttccc taaagccccc gtagctctag gtccagctgc cctggcaatg 14340
cttcttcgcc tgcacaactg gcctcagcct ggcgtcagcg ctctcattgt gtcctgagcc 14400
cacacagggc accaaacctg cccaggtggt gccctggcag cacaacagcc aggtgactcc 14460
agcccacaac caaggcctga atgggtaggc agggcacatg cagccaccct gctgctgggg 14520
aaggcaggag gaagagatgc tgggagcacc acgtcaatgc agaccccgcc gcgatgcaga 14580
agcaagagca acagcagcag gaaaggagag ggggagacag acgtagagca cagctaaggc 14640
aggagagagc tcggctccgg ggtgaggcca gcccgacacc ccgacccagc tcagcctggg 14700
agtctggctg cagacattgg gggcccgggc agaatacatc atgcagacgc ccgggcgggg 14760
gccacctggc caaagcaatg ccctcagcga ccaacgctct acctgctggg tcgtcggttg 14820
ggaggggctc ggcaggaagc gactcgactg gtgtgggggc cccaggcaga gggcagggta 14880
gacacagagg ggatgagagg agagagaggc acagacatgg gctttcaaaa caaccaaaat 14940
gcacatgagc aacaaggact cctgggtctc cagagccaat gacttaggga agcatttgca 15000
gaagtcatca aggccatccc cctgcctcca gacagcaaca tctatttgct aggctctcta 15060
ggaaagatcg cacagccccc gccgcccccc cttcaccctt ggtcttctct tttttatcag 15120
agctgcaggt gctggcctat accacagcca cagcaacgca ggattggagg tgcatctgca 15180
acctatacca cagctcatgg caataccgga tccttaaccc actgagcagg gcccgggatc 15240
gaaccctcat gctcatggat actagtcggg tttgttacca gtgagccaca aggggaactc 15300
ctgcccttcg ggttttgata actatccagc aagctcttct ttaagtatct aatctcaatg 15360
ctttctgctc tcacctagat cccaagacag ggaaaaacaa cctgtgaaag gtgcaggcgc 15420
ggataagacc tggtggagag tgcatggcag atacccaggg cccccaggag agcaggtgag 15480
ctggcggggc agccactcac ccttctgctg cagggccagc tggcgcttgg tctcaatgtc 15540
ctgcagtgtg gctgccaggt tgcgaggaag gctgcttgtg ccattgtggg agagcagcac 15600
actctgctgg gggcgggcag ggatatggaa cgttgagacg gatccaagtc ccctcgcccc 15660
aaggctttca tggatggctc tggcctctcc cctgcgtggc agtctccttc cccctaccgc 15720
cgccaccctg ccacatgcac agcccaccca ctgtcctcag acctttgggg aggggctacg 15780
gcccaaggta gccacgctga gctgggagga ggaagaggaa gagccagagg aagaggggag 15840
ggggccactg cgggtccggg gcagggggct tgtggcttca ccatccatcc tggagcgata 15900
aacctggaag gaagagatac tgcctggtat ctccagctcc cccaccggtt gccaccccca 15960
tggcacctag gctggctgtg acccacgata ctgcccatga ggtctctgtc tctgacaaca 16020
gcactgagtc tcctgggcca gcccaatggg cagatgctat tggcagctgc caggcctctt 16080
ttccaggctg cttctccttg gactaatccc cggttcaacc ctgcatctta gaaaggacct 16140
cgtaaatcaa cccatgtgaa gagttaacat ggatgggcag agtagtacag gagcctcact 16200
ggaggacggg gcgccctctg gtcccagctc tgctacctaa caggctgtgt gacctccggc 16260
cacgcacctc cccactctgg gccttggttt ccttatctgc gaaagcagtg ggaaggacta 16320
gactaaacaa ttgccacgct ccctcccagc actcatgttt ggtgaatgtg gtctttggag 16380
tcacgaaccc ggactctcag tatagcccct gctacttcct agttgtgtga cctcaaggag 16440
attacttatc ttctctgggc cccagtgttt gatctatgaa acaagagtta tcattatcta 16500
cctcacagcc ctggggtaat gattaaataa atcaaaacgt gtagaaatct ctaagcagcc 16560
aggcccaaag cagaagtgcc atacagactc ctcaggaggg aacacggact ttcagctgag 16620
atcacagtct tagaggcgca gtgtcttcat tcaaggaatg gcttttgaat gggtgtctca 16680
acactacagc tggtcagtgc agtgaaactt ggggtagaag acgtccaaat gcccttccct 16740
accccctggt cagcagcttg ctgcaggctc aagcaggccc ttgcccccca tggcaactct 16800
gaggaggctt ggctgtgtcc tcagggctgt cacttcagag tctttgtccc actctgtctt 16860
gcctccaaat gtcacccccg gggagactag gagagcaaaa tactggcacc tctcctagga 16920
cacactcatt ccactgcaac agactcaagg gtgtgtcagg cccccagacc aagtgagaag 16980
ctctggggtg agggctgtca aggacagagc caagcctgtc tagctcagca cgagacacca 17040
ctgctcccga accccttcca tgggacagtc aagcatgtca ccctcggact caggaggctg 17100
gctccccaag ccacccacgg ggagaccctc actcccctct cagggtctag aggcccctgc 17160
tgccctggct ggattgaccc ccaccatcta aggtagcact gggtggctga tgggtcagca 17220
ggaggtaacc agagcaaaga ggagcaggcc tgggcagggg tgggcagtgg agaaggggtt 17280
acctgcagct gacgggaagc tttggcgggc agaggcgggg gggaggagcg aggggaggct 17340
gcagcggggc cagtccccag cccggccatc agctcctggg accactgctc taagtctaca 17400
gcggggagca gcagcttctc catctggagg ggtcacgggg aaggggacag gggacaggag 17460
gggaaggatg gagaggaaat gtcaagaaat gaggggaaag gaatagggag gaagaaggaa 17520
gacaggagat ggttggagga agggtagaga aaggccatca tcaggcaagc aggcaggaag 17580
gtgacgggaa agggagggaa gggagaaagg agagcgtaaa gagaagaaaa cacggtgaga 17640
aaacagtggc cgtggatgcg ctaagggcaa cgagaaacaa ggcataactc ccagaccaca 17700
gacacgtgct ctgccaacca cgatgccagg cagccagagc ccccaggcac tcagcactcc 17760
ccggggccag gcgagggccg agatgccagt acacagagca aggcacacac agacggagtg 17820
ggctgcgggg gcgggggggg ggcaggaaca caaagaccac caacctgggg cgaaggcgtg 17880
acagaagcca aggaggagga agagggcagc acgggaggaa ggcaggtggt gggaaccagg 17940
tcctgggagg cagaggccca gggaggcagg cccggcgcag gggctgtggg cccgggcgag 18000
gtaccccaca tcgcctttag ctgctcaagc gtcacgtact tgggggaggg ccaggcgcgg 18060
gagggaatac aagaggttaa tgggacagga acccaagagg cggccctgcc cgtgggcaag 18120
ggaagggtaa aggcaggatg cacggagcag aggaggctgg tggagcccga gaaagccagg 18180
ctcctctcag aaggatgccc tcgcactagg gcaggccctc cctccttgag ccatcaggag 18240
ctgaaggaag ttcccaaggc tctgggggag agagagggaa tcccacccaa cccccctatg 18300
ctagcgccct tctccctcac tgtggaagac agacccggct gcccccaacc caggcctgag 18360
ggacaatcta gggcctccgc gaggaggccc gtgggcatag atgggctcac tcagtggctg 18420
gtacctcact gtcaggcagg ggctgggcga gcgcgctggg ggaggtggca gaagcaaagg 18480
gttccaagcg ggagcacagc tggagaacct ctgccaggct cacatactcc tagggtgccc 18540
agtaggaggg ggagggatgg gcagagaaca ggttaacagg attcaaccag gtacctgata 18600
cccacactcc atgccacaga gccatcactc cactcaatac agaatccaga gcagggcggg 18660
ggagctcagc tgcccactgt ggccacagat ggctgtgggg aaggaggggc aggaaaggac 18720
aggcggaaat gactgtctgg aggctcctga actccacttt tgaggctgcc ttagcatctt 18780
gaaggcccat tcctgggttt gcccttgact aagggctgaa gaagcctgag gaaggtcttc 18840
caagagtcca ccaaggacca ggagccagat cccagagaac ccttctgtct ccagtaccct 18900
gggcatccca aaaaaggcat acacatgcat cactgggcac cagtcacacc attcctgagg 18960
gacctctcgc atcccaccag ctggagcaag agctggaagg tgtgggagtg gaggcactac 19020
gagggcctga gcaagatggg ccctgagaaa gggaccttag cagggagcag ctaaaagccc 19080
aggtaggaaa aatgggccca cttcccaaag gttagtactt gaaaagatgc taacacacta 19140
ctagactgtg ggttggaaag acagggactg tcacactccc tgagcatatc ctcagaatct 19200
cgagctcata tctaggtgct aaggacacag caggtatcca aaatgcatgc agaatcaaag 19260
cacagaacct aagaccacct cagccagggg ggcccatgct ggcctagccc cggaagctga 19320
ctgtcctcca ggagtcatgg aaaaggaaaa tgagcgggaa ggtccagtgt gttatgagag 19380
ctgatggaag gcatgcagaa tacacatgaa tttaaatgcc agtcgatgct ctgatcttgc 19440
accggtggcc caggcaccta tagaacagca ctctcacaaa ctctggccag acgagggtag 19500
actgccacac gccctttagc tgggcagttg ggaggaactg tgagcagaag ctcagcaagc 19560
aggcagacag gcagtgagca cagcagagca ggcagttagc tgtgattacc tcttgcgctt 19620
tggggcagag ctcagtggaa gcaggtgggg ttaaggggac agaggaggcc ccagcctgag 19680
gagaccctgg gaaggggccc agagccaccg tccccagccc catctctgag gacgcggaga 19740
tgagcggttc agcctataga gagggtacat gtgaggggcc tcagccctcg ccctcagctc 19800
agccacccca gcccaggcct aaactgctta tgccgggagg cgtgtcctgg ctggacctcg 19860
ctcaccaggc acgcaaagac tcagcaaaga atggctgaag cagcctcaat gcccacttca 19920
agcctgcagc ctagttccag acagaagccg ggggagctcc aagtttagga ccccctagac 19980
ccagccctgg ccctcgcgaa gcctccaaga tgccatcctc cccacctatg cctcctacat 20040
cccccaggag tattcagtca ccccacatgg taagatcaga gggctcctgg gagccagaca 20100
gggagctgaa aagcagcagc tgggagccat agaggatcca gtgccagacc tcagggctct 20160
ccagctcagg tcctacccag gtctctccag ttcaggaaaa cgtccccata ctcgacctca 20220
gccagtgggg tgaaggtgcc acgtgttgct ccagagcctc ccaggagagc acttgggcct 20280
caggctgggg ctgatccaac cacattacct ctttgagagt cgacgtggtc ttcgggaaag 20340
gcctgccccc tgtgagtgag tggtatcttc gttccaacat ggccagtttc tccttctcct 20400
gcaaggccac agtggcagaa tcagaaagga gtgggagtga gggcaaggca gcccaaggtg 20460
gctggcaagt ctccacccta gcccttctgc acgcccctcc cgctcccccc agcccctccc 20520
tagccctgct tccctaccca acccggcagg acactacctt ctgcagcagc tgcaaggagg 20580
cattcttatc ccgggccagg cgttcagact cttgcacagc ctgggaccgg atctgcccag 20640
cctgactgtc caggaccgcc aggcgttcct ggaggggatg agggttctgg gtcaggccct 20700
ggaggaacac agggctagac aagaggtgag gtgaggctgt ctctcctgcc cagagacctg 20760
ggctggtgaa gagatcacca gctcgtcgcg ccatgagttg gnnnnnnnnn nnnnnnnnnn 20820
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 20880
nnnnnnnnnn nnnnnnnnnn nactctgtcc cccggccttg gagagctgcc ncttttccgg 20940
cactccacct ccacccggta cacgctaaaa tcacacctca aactacaatg gtacctcgtc 21000
ctgccgcctc ctccagcctg cacgcctgaa gtcgctcccc actcctgggt cccctggctt 21060
accctccggc cccctctttt cctccagggc tctgcctctc gcctctcttc ctcccctccc 21120
tgtctccccc gccatggccc tgggtacctt cgactgctct cctggtcagt cacccccttt 21180
ccttgaggac tcctctgtcc ccagtctccc ccatcgcctt cgtcccttcc ccatcccttt 21240
acctctctgt ccctgtctcc cctcacttcc gcatccccca gactctatcc tgtttcccac 21300
cttcttccct cctccctgtt ctctgccctc agtcttccct ccccagctct ccttttcctt 21360
cattcctcag tctccctctt tcacttttcc ctccaaatcc cttcgtgttc cctgccgccc 21420
ggtcccttcg cagactccct cgatttcctc ctcctgaagt cctctctgcc ccagaccttt 21480
atccacgcct tccccagtcg ccgtccggct ccgacgcccc gcccgccgag ctcccggcct 21540
cagtttcttt tccttcccgg ctccttcctc tgccggcggc ccttctttta gggcggatgt 21600
gcgccgggca ggcgaaggcg gtggctgccc gagaggatcc cagctcaacc ccgcaggcct 21660
cgggctacag ctatgcgacg ctcggggcga ggcctgggct ggcggccgcg cgagccacag 21720
gtgaggccca agcgggagca cccgagcagg ggagccgcgg ggattgctct ccggggttgg 21780
cctcagacct ggacttttcg tctggagccc aatttggagc ctggacttcc tctagtgcct 21840
cttagttcca gacttttccg gccgctaccc gggcctccca ccgccctcgt tcgggtgcca 21900
agacccccga agcagtgact ccaggccgtc cacgcggcgc agagtgctgg ggaccgggtg 21960
gggctgctga ttccccgtgg cctggatgcc cgctcgttcg cgctggggtg tgtgtggggg 22020
ggcgaaacac ggtcacttgc gggagcctgc gcctcgtccc ccaccgaggc ctcgagacac 22080
cccgacctct gctaccctgc gccccaggag acgattgggt atctgcccgt ggcgcgggaa 22140
gtccaccgag agctgggtct gaccgcggcg ctttccccca gtgtcttctt ccccagagca 22200
gtacgcgcac cgcaggggcc tttttttggc agcgcgcgga gtagggtttc ccagaaccgc 22260
cgcggaagcc gggcagggag agcgcggtag gtaattacgg ccccggggag taggcagccc 22320
tgtgggacgg aagtgggtcc tccagtgggg ggcgctccgg cgcagcccgc tttgccctcc 22380
aggccggggg cggtgggggg tgggagagag ggcgcccccc ggtggcggct ctcccggcag 22440
cggcgcagcc ctgggtcaga tcgccgcctg agtgctgggt ggctgggctg gtgtatcttt 22500
tccacttcac ctggattagg tttcagagct ggggccacag cctcacgcct cccaatctct 22560
acatcttttc ttatcttctc ggtctctctt acacctctct ccttatcttt ccctcttctc 22620
tcacctcctt attcccgtct cttcacttct ctaatcactc ttgttcccca tcaacttcat 22680
tcctttacag tctttatctc cacttgaaat ccttggtgta aataaacaaa cacacccact 22740
gcagcctcat agggcgatag tgacaaactt gacaggtgaa gtaactgaag cccagaaagg 22800
tgaagtgact tgttcaaggt cacgaagctc atattaacta ctggcatgtc cgtagtgctt 22860
tgcacttcat ggagtgtcat ttgatataat acctgaaatc ccagacaatc ttttgttttt 22920
ttactaacaa tttttattga ggtatgatgt tcataccata aaattcactt aaattaagtg 22980
aacattctat gatttttggt atatttacag atctgtgtaa ccatcatcac aaataagttc 23040
tagaatgttt ctgtcacccc caagagaaat atgcccattt atagttactt cccattccac 23100
ccctagtccc aggcaaacaa taatctagac aatctatctt ttttctctaa atcattattc 23160
caggtgttct cttgtggtgc agggggttag ggatctagtg ttgtcactac agcaacttgg 23220
gttgctgccg tggtgtgggt tccatccctg acctggcaat ttccacgtgc tgtaggtaca 23280
gctcccccaa tccccaaatc attattccag ttttttattt ttagtttaga cccacagcat 23340
gtggatgttc cccagccagg gatcgaacct gcaccacagc agtgacccaa gccatagcag 23400
tgacaacacc agatccttaa cccattaagc caccaaggta ctcctattcc agttctatag 23460
agatggtctg acactcagaa gttaagtaac ttgtccaagg tctctcagct aatataaaat 23520
cagggtagat ttaagngtct ggagtctgtc gtgctcatgg tatgactgac agctctatag 23580
acctggtcga cctgactcat catggtaaga atgcatgtct gactggtgag tgcagtcacn 23640
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 23700
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna aaanaagatt tttgctcttg 23760
cattcattta aagttttcta aatgcggatg ccttcctcat ttttctgttt gtttctctta 23820
cgccctcttt taataaactt ttcatccccc acccactgct ctggcctctc ctcccttgag 23880
aatgctgagg gatttccctg agtcaaagaa gcagtacagc agtctctgcc ctggaaattc 23940
ttccccctgg ccagatctcc tggggaggtg ccaggccaac ttgagcctta gtgtaggtta 24000
atggccttag ccttccctct tcccaggccg acttggggtg ggccccaggc cctgttccgg 24060
cctcttttcc gccagctcgg gtggaaatgg tgacggaggt gatgcagtcc aggtccggaa 24120
gggtggaagc tgggagggta aaattagtaa ctctatggta aaggcctttg agtctcactg 24180
ccagggtcag acactccttg gaatgctggc tcctgcccca ctttctctcc aagtcccttg 24240
gaagagaaag tggaggggaa ccctgacttc ctgggtctcc ttctttaact tcagtcacat 24300
tattttcagc ctcttcacat ttcagccaca aatgggaagt ttctcacttt agcaagctgg 24360
ccaagggaca ggcatgtagg cacatattaa tatgcaaaag tccttcctca tagttctcag 24420
gtaccaaaac tgtgagccag ggagaaaggg atggaaggga gagaaaggga ggaagcattc 24480
aagttctggt tctggcatct gtttgacctc tgtttctaag gacctaagct tcctatcgca 24540
ccaaatcagg ttaagaactt gagagtcgta tattccagag ttagtcaccg gtgagttatt 24600
gttttgtttt gttgttttct gattgtgtgg gggtaagatt gctttctggg gaagagaggg 24660
ttcaggaatc aagacaaaaa gatggcagga gtagttgcat tttccccgtg tgtctccttt 24720
aagaaaacat ccttctgaga ccccccccca aaaaaaaaag agagacaagg gagaagaggg 24780
gaaggaaagg ctgcgggagg gagctcctgc tcagccctgc cacccacctg gggccctctg 24840
tctaccagtg tctctgaagg ggcttccttg gactcacttc tcccactaaa ttcaattttc 24900
cccactccgc ttgtgggtcc tcagctgaca aggcctgggt cctgggtgtc agagacaaga 24960
aagcacagag ttacaggttg ggccctgtag agctttttgt gcttcctgtg tgtggcaagg 25020
gccagggctg gggtgacatc cacgccttgg ctggagctgg ggcaccgcct tcccccagga 25080
agctcccgag ctgcctccac ttctccctca gcaggagtga gctgagaacg atggtgactg 25140
ggctgccctt ggctggagaa gccagctgca cagtctactc cttgcccttt cctgccctgg 25200
cctcccacct cacctgcttt ctgctgtgtt cacaggagct ctggagcagc aggaccatgg 25260
acactctcaa taggagccag gtgggccctg gatgcaagac ccaagctaca gtgcaggtga 25320
gtggggtcag agccatgggc cagagagctg acggtgagga tgggttggct cctcttgcta 25380
cctctaggcc cagcctaaag atgagagtgg cagtacctct ctgctcttcc ccaggtccca 25440
agcctattaa cagctgagtc tgtagctgaa ttaggttaat tggttcaccc agaaaatacc 25500
ctttgaaata cctttttttt tttttttttt ttggtctttt tgtctttttg cctttcctag 25560
ggccactccc acggcatatg gaggttccca ggctagggat ctaatcggag ctgtagccac 25620
cggcctacgc cagagccaca gcaactcggg atccgagcca cgtctgtgac tcacaccaca 25680
gctcatgata acaccggatc cttaacccac tgagcaaggc cagggcttga acctgcaacc 25740
tcatggttcc tagtcagatt tgttaaccgc tgagccatga cgggaactcc tgaaataccc 25800
tttgaagcag tttacaaaat gaccctgagc cgtatctcct ctgcgatccg tgtcctgccc 25860
tctcttgtaa ttttgtttaa ctgaggccaa gtggattgcc atggacaagt ctacagggac 25920
aagtcttttt tttttttttt tttttttgct ttctagggcc tcactctcgg catacagagg 25980
ttcccaggct aggggtccaa tcagagctac agctgccggc ctccgccaca gccaccgcaa 26040
tgcaggatct gagccgtgtc tgcgacctac accacagctc acggcaatgc tggatcctta 26100
acccactgag caaggccagg gattgaacct gcatcctcac ggatactagt ccgaccattt 26160
ctgctgagcc acgatgggag ctccctttaa ggacaaatct tgatcaaccg tcctctttgt 26220
atctgttctt tgacccagca cctccccatt ccccttactt tctccttgtt tctgtcctaa 26280
tatagacaaa aggggcagtt aaatcaggac ttgagataaa tccagtggga ctagttagtg 26340
gcctcgattc agatctaatt tcaatttagc ggaataacgc tctgtgcaag attttgtgct 26400
aagcctttcg ccaccctcag agccctttag agagggcagt gctggagaga gtgctcctgt 26460
gagtgggact agggtggtga ggtgccagcc tggagactct catgatgctc atcccccacg 26520
tccgctgcct gcagaaagga cccttggacc tgattgaaac aggcaaaggg ctgaaagtgc 26580
aaacggacaa gccccatctg gtgagcctgg gcagtgggcg gctcagcacg gccatcaccc 26640
ttctgccgct ggaggaaggt gagtctggag gggcaactcg cctgctacac ctccatcctt 26700
atgccattcc tgccctggaa tccaggcctg tgctggggcc ctcctgctct ctgctggcag 26760
agtttgcctc cctcaaatgg gagcgtgcca ccccgccctt gcttgcttgc ttcccctgcc 26820
tgtgcctccc tagaccgctg cgctcttggg tggctaatct ggctccctga ctaggattaa 26880
agatcagttt agaaataagg aggacagcac tgaaaaagga gagcacatgg cccttggcaa 26940
tcagtgtggc caagaagaat gggcacccca ctccagggcc atgcctggca tgggagaagg 27000
tgctggtaga gatggagaga ggggagtggg gcagagttcc ctccgtgtct ctctctctct 27060
ctctctctct atctctctct ctctctctct ctctctgtct ctcagctcct gcccttcctc 27120
tcttctctcc tggctgcact tgttgcccgg gtgatactac attctccgca ttctccaaat 27180
accagtcccg ggggctctgc cagggggagg ggctgtgcct gcagaagtct ggagagaatc 27240
tgccctgaag agagggaggg agggcggcgg gcaagggttg caggtggcag agggagggag 27300
ggaagaagga gagagggtga gttggaagaa aagggggggc aatggaggag aaaaaggctt 27360
tagtgagggg ggattggagg gactaagctc cccttccctc ttctgtctgt ggatcctctg 27420
tgcccactgg gccttcccat ctctgggccc agagaatttt ctccaccaaa gagcttctac 27480
ccctgattgt ctccaccagg ctcaggggcc ccagtgggtg gccagtgact gtggcaatag 27540
tttccagtgt ggcctctttg ctgggttggg tcccccctgc ctcctcagct tggaccaccc 27600
cctgactcag tttgcttcct gtcttcaggg aggacagtga ttggctctgc ggccagggac 27660
atctcactgc agggtccagg cctggctcca gagcattgct acatcgagaa cctgcggggg 27720
accctcaccc tctacccctg cggcaatgcc tgcactattg atgggctccc cgtccggcag 27780
cccacccggc tcacccaggg taaggtctga caccctggaa ccaagggagc ctctgtttaa 27840
gagcttgtgc acagcaggtg caggggaagc tggccatggt ggttgccata gtaactgcct 27900
tgagtggcca agttgggggc ggggtgcttg gtgcatgtgg atcaggtgtg ttgggaaatc 27960
cctccagtat ccagactgtt ctccaacctg cttttctttg gtcaccccca ccctgcttcc 28020
cacaccacgg gcagagaaga ggtgctgctt cctgctatta gagggcatgc ggtgtggcac 28080
agtggtcgct agtggtcatt aggggacagc agctgcagca gcagtgaggg gaacagtggc 28140
atggatgcca agagggagtt gttttgaaaa gctgttgaaa ttgggacgag caggacaatg 28200
gggatgggac aagggcaaag tctgatggca aaatctgagg acataacccc tccagttgac 28260
agtggctttg gggtcataag tacagttacc acctgttcct caaagcaaca ggccattttg 28320
ttgtgtagag cagccctaaa aactgggctt ctagttactc tggtgaccag gcagataaaa 28380
ggttgaccca ggtagcactc tggtggacca gttgtgtttg ccctgggcat cagtgtatac 28440
agtaaatcat catattaggg gaggtgagaa aagcagattc gggtgggagt agaggatgag 28500
tgctttggac caggcctggc tctgagtaga aggactgatg tcttggcatt ttccggggac 28560
tcttatgtct tactgctgga agctcctttg cagtagaaac cccactcact ccactatttc 28620
tcccctcctt ctcttcatac cctttggggt ctctccctgc agagctgatc caggagattt 28680
ctgcctttgg gagtgtgtgt gtggtggggg ggacagctgg cttcagggag gcaggtccgc 28740
tagcagcagc agcagcaggc aggccctggt ctcttccagg acttctctga ctccagataa 28800
gaggaggagc ccccataaac tggggctttg gctgtgatgc catctggctc ctgacagtcc 28860
tcttcactct caaagacagc accttcccca agatttgggg acatggaagt gagctattag 28920
ggcagcaagc agaggcaggg acagagtgca gccccaaatc cctcacgttc actggcaaga 28980
atgttcttcc tctgtcttgt gagcagaatg ggggaactga tgctctgaag gcctctcttg 29040
ctcagtcttc ccagtgcagc atttagctcc ctgagcagag gaagggcctg ggccaagatg 29100
ggcttcttag gcatttcctg tggcctgagt gacccccaac tccttcccag ccaaaagccc 29160
accctgccaa gtatgtaccc tcccactttc tcctgaccct aaaccccttt ccctttggct 29220
ccctcccacc cctatagctg ccttgtctag gctcgcctgt ctcagaagct tggcagcctg 29280
gggaggggta ggcagaggca caaaggaacg ggtgttcttc tccccagcac tacccctccc 29340
ctgggctcca ggagctgagg gtttccttgt ctcatctctg gagcaaggca ccaagcccag 29400
ccctccctgg ccccactccg gacattcttg ggagtgcgtt tccctggaaa ctgggtggct 29460
cgggggtgca tgtgctatct ctgtgcgtct gtgtaactgg agtgtgttta tgtatctccg 29520
gatgtttgtg actcagggct tgtgtatctc tgtgtttgtg tctttccttg tgtttatgga 29580
tcagagtgtt caagttgggg gcaggtcggg gtgaacacag gactatggtc ccgaagtatc 29640
cctgtgtgtg ctcctgcgtg tatatgagaa ctcactggcc acgtgcctct ctgtgaacat 29700
atgtgcaaga ggatcatttt ccctgttttt ttcccccaat ctacccttgt gggggggggg 29760
gaccctcctc tttgaagaac agaaggctac cctagaaata acaagaaccc ctctccggct 29820
gggccaggcc ttggatgcat cattctgggc actgtgccca gccataggca acttggagag 29880
gcacacgtca taacgagcag agtggcagtg acagcctctt ccagcaggaa tctgcctggg 29940
ccaggggtga ggggaagcag gcagaaaaca gatgccaacc ttgagctcga gctgtggaca 30000
tagatagcag cctgaagtga gggagccgga aggatcctga ttccagtctc atgcctgggt 30060
atcttggtac atcccatgct gccttctctc tgcagagcaa gaggtggggc tgggaatagg 30120
accaaggaaa tgaggaggaa tctcaatctg ggtctagcct gccccgccct gggaccacat 30180
cgccagacca catgaaaggt ctgggagggg accaagtgga ggaggaccag aaagccatgc 30240
cactcagggt ccaggctgtc tagacagaag gtccccatct tcacccagca ttcgctgcca 30300
gcccttgtct ctgcgtctcc atcactaacc aggcccctcg ttggtgggca ggggcttatg 30360
tgagggctgg aggggaggct cctggaaggt ggctgcagag ccaggatttc cgggttatct 30420
agtggcctcc ctttgggatc cagggcactg agcctctttc ttttccactc tgctgtcagt 30480
tattccttct gaaaaatggg agtgaagcct ccaactgacc tctgttataa gactgataaa 30540
gatcttgact aacaaagctc aaaggaagga gagaaaaagc gaaagctcct agctcgccac 30600
gtctctgtgc ctgcctcctg ccctaattct ccctggcggg catcctccgt gtctgtctgg 30660
gtctgtccag ctggccgtca cagtttccag accgatgaat cacatttcct gaggacgttt 30720
ttttttttta aaaaagcctc cccctatttt gcatttggcc ctatatggtt ttcctttttc 30780
cttcagaaga gaaataagct gcatgcgtac acacgagtgc gtgagtgtgt gtgtatgtgt 30840
agtatggcag tgtgggtagg tgttcaacaa gggtgaaaaa atcatttgac tctttctcta 30900
aggaggttga ttctggattt cagagtcttt ccggggactg agtgaaagac tctctgtgcc 30960
tgagtgaacg tgtaaccaag agggaagctg gcgcctgtgt ggttgtgtgt ttgagaacaa 31020
gcgtgtgtga gagagagggc atgtttaact ggggtgtctg tgtgactgag caggtgttgg 31080
cgtatatgtg agagaaagtg tgtggggcac acaaagcact tctgagagaa cgcagttttg 31140
tccctgaagg ttctggtgtc caagaggcta ggtccctgaa atgagaggac cttcctgggg 31200
cctgggctgg gtggtaggtg cttgcccggc ccagaaactt ggccggaccc tgggggctcc 31260
agggaccatg gcactgtgtt tcctgggtag gaagggcacc ttcttgggca cctccgctgg 31320
ggctggcaca actggtggtt ccagtggggg tcatggggtg aggtgagctg ctgggagtgg 31380
gggaatgggg cgtagggggc agaggaagag gtgtttgggg tggagatggg gccatgctgc 31440
tcactttgct cagaacactg cctgggcctg aactcccacg tgtcacagtg gcagactgag 31500
ggccagagct ggatccaggg accttgctgg attcctggga gcttcactct tctctctaat 31560
agatggagcc atggaatgtc agagtttaac ttctgctgcc aatgaagcca acctcttcac 31620
tctgttgata aggaatcagg cggcaggagg gtgtgactgg cccaaggtga ccagccagcc 31680
aggggctggg ctggccccat cctaggctct gatgacttct aaccacagag ggctctcagt 31740
ggaaccccac ttctcctccc gtctgcctcc aggctcttca ccctctggcc tccgtatcta 31800
cctctcagct ttcctccttc ggtcatctcc cctgaacctt tcctccctgc cccacttctc 31860
cacctgaccg gcgctccgga atccccgggc tcgctccctc cccgcatcct cccgccccgc 31920
cccgccccgc cctctccatc ccgctcccct ctcttctcct ccatcacttc ctcttcccct 31980
cctcctctcc tttgtccctc cctccctggc tcccttcccc ccgccgccgg tggccggccc 32040
gcccctcccc gggcattggt ggctgctcac cgccgggctg ctcgctgctc cgagcccgga 32100
gctggcggca cgggaggtcc gcccgccccc tcaaaggccc ggggcgggcc ggggacccgg 32160
agggcccgag ctagcgggga cgagcggtcc ggaggtccgg attcggtggt gagtcctgag 32220
ccccgggcca gccgcagccc ggcagccggg cgccgagcag ctcagctggg aggggcgtgg 32280
agggggtggg gaggcgcgtg aggagtggga tcagatggtc ggcccgtctt cgggagctga 32340
ggctgggaga cccgatcgtg cataagccct ggacattgct gagcgaggcg tctgggcttg 32400
gagccgctcc acagcggggc agacttcatc ttcctcagta ctaggtggtc cccttcgtgg 32460
gacctgcctc tggatgggga aaggggtaga ataagggggg cctgtgttat ggctaagagg 32520
ggtgtccgat ggaagggacc gagacccttc ctccggtctt cccctcaccc ggcctccttt 32580
tggccagttc attccgtccg cgtggccagc agggcagtga ctgtggctct gagtgtgtaa 32640
gcccatatgc ctatttctat atctgtgcga gtgcatgttt ctgagagtgt atgtgtgagt 32700
caggatgaga gtgtttgtgt gtaatgtttt ctccccagtc tctcagggat aggatccacc 32760
ctcttctttg ccctgttttt tccccctcca cttgtgtagt tcctccacct acctgctcac 32820
agctgggccc tgagcccctt tgaagtgtgt gtgtgtgtgg ggggcatgaa cacgactttg 32880
cctggatgag agggggttct tgggtttcct gaatgggcat gacctgtgga ggtgtggagc 32940
aggagtggac agagaggtgg tttcttccca gaaataggct ggccaggccc ctgggcacat 33000
aggacctgag ttggggcatt gcctggggcc cctgagtgca aatagaaagt taatttgggc 33060
aggagctggg ctgcaatcag tcctgctgcc ctcctgcccg ctctattaat acagatccag 33120
ctctgctgcc tggcatcacc tatttatagc cccagaggca gcatagccac accccttgtg 33180
cagaggaggg tgaggggcag aggagggggc agggaactca cagctatagg agctagactg 33240
agggtccgta ggtgcccact gggtgggcct ggcactgatg cgctggaatc cacccagact 33300
ccccacctcc ttcccagtct ggactgttcg gtcagactcc tgttccacac cccccctcca 33360
accccatcaa tctagggttg agtaggtctg tctagaccct gcgaagtcat cattaattag 33420
cttgttaaat ggctgaaagg agggggaggg gaatggccgg gctctttctg aaacagctgt 33480
ggttgtaatt aggtggacgg tgtggctggc tattatttat tctggttatg attgctctag 33540
gatcagctcc tctgattggt ggcttggccg ggaaggctgc ttcctctcct aaccacccat 33600
cagcctttgg aattgttggt gcccgcctgc ctgggagggc ctgtgggtgt gccagagaag 33660
tggacggttt tgtctactat gttatctgct atgggcttct cagcctgcta gtcccccatc 33720
tatggcaatg cccagggttc ctgagcttct cagtaggggg cctgccgagg tacccacatg 33780
gcctgggatg ccccatgccc tcgtggcctg cctaagctgc actgtctcag cctccaggaa 33840
tgtagatttc cctgtgagtc ttagctcaga ctggcatgaa gcttggggtg ccaagtggca 33900
ccagattggt tagacagggg ccaggggcca agggtgtgga cagattggaa gggtggatgg 33960
aagaaagcac cagggctctg gcttctccca gcagagcatc ttgtgccgat gccctctagc 34020
acttgcatgg ccgggaagag gagctctgcc agccaggctt aggcgcctgt gggcacacct 34080
gctgctgggc acactcctgg tgcccctggc tacattttct ggctcccaca taccctcccc 34140
gctccatcca cacacacttc ctgggcagga ctgggcttgt gtctactgcc tgggaatgtg 34200
cttggctgag gccgggccca ttggcctagg cttggccgca ttccagggga gaccaagggg 34260
caggaaaatg tcaggccaga gagaagggaa gcaaatcctg gtccccgcgg gggcatctga 34320
ctgatccatg gtgtttccct gtcccctgtc tcagagcttt tttgagatcc tggaagttga 34380
tgcttctcag actgactctt ccactaaagt gcagatagat ggatcacgga tacaggttta 34440
gggggaagca catgggtgtc catactctct ttttttaatt ttttaatttt tttttgtctt 34500
tttgcctttt ctagggctgc accctcggca tatggaggtt cccaggctag gggtcaaatc 34560
agagctgtgg ccactggcct atgccagaga cacagcaacg caggatccga gccgcgtctg 34620
caacctacac cacagctcat ggcaaccccg gatccttaac ccactgagcg agaccaggga 34680
tcgaacctgc aacctcatgg ttcctagtca gatttgttaa ccactgagcc actacgggaa 34740
ctccttgtcc tctttttttc tgatgagaaa actggaccta gggaggcaaa gtaacattac 34800
ctaaagatct tatagatatt aagaagaaaa tttaggagtt cccttgtggc tcagtgggtt 34860
aaggacccag cattgtcact gttgtggctc tggttacagc tgtggcttgt gttcattccc 34920
tagtgtggga acttctgcat ggtgcagatg tggcaaaaaa aaaaaaaaaa aaaaaggaag 34980
aaaagaaaat tctttaggac tagggctcac aaaccagtgc tctttctgct cagccttgca 35040
ctgtggagct gggctggggc ctgtcttggg cacaggcaga gtgaggcatg gaccttgagg 35100
gtgctgtgag tgcaggtggg gatggctccc cggagactgg gatgagcctg cccttcccag 35160
cttttgcttt ctctgcaggc tgcatgttgt gcctgggtca gtctaccttc ctccgcttta 35220
accacccggc tgaagccaag tggatgaaga gcatgattcc ggcagggggc cgggcccctg 35280
gccccccctt tggtcctggc ccaggtaggt acgcaggtgg ggcaagcggc tggtttggtg 35340
gcaccccaca gccagtcctc cttcttgccc actccagcca aacttgtagt gtcagggcag 35400
gacaataccc gtgatctggg cttgctgcca cctcccatgg gcccactggt ggagagtgcc 35460
aggcagtctg gccactggga gagatccaag aagtactgct tgcctggaat tgggaatcat 35520
gtgcctgaag gatggagggc tctgccagcc tgtgtcccac tgccctggaa ggtgccccct 35580
tagtgtctgg gaccctgact ctcttgtgtc accacaagac agaagagctt cactcttgag 35640
ggtggcctga ggaggcagaa ggaagcatct gattgagctc ctgaaagttc tggagggtcc 35700
cacctgtttg tgatcatggc cttgggtcct gccccacccc caggccatgc catgccagct 35760
gatgactcag tggagcagct gcagtttgtg tgtgaattat tgatccccgg aggagagttg 35820
gtgattgtct cagggaacca gcacagcccc ttcagcgcag ctgcctagtg ggtggggtac 35880
aggccaggag actgcctgta ggcctgctgc cccagacttt gcacaggcat tcagatggct 35940
gtgggatggc aagtgtcagc atggtagtgg gacaagtgga catgcatgtc aggtacacct 36000
agtgggatgg catcagtggc ttaagtggat agtctaggga tttgaggaaa gatgggcagt 36060
tactggcatc atcagtatcc atagcgaccc ctctgatttt ggtctcacac ggtccccaca 36120
attttggacc cagactcggt cttgcaaagg ctttcccttc tccttcaccc cttacttttc 36180
taggcccttg ggggcgtgga agcctgggtt ccaagtccac tccttgggta gcttcaccct 36240
gcccccaccc tagccttgtt atgccgctcc ctcagctgtt tggggcagtg gttccaggga 36300
ctcctggttg ggtgacgagg tctggggcag ggcaaaggca ggcttttgct ggcgtggctt 36360
aatgggctaa ttggaggcac tgggctgcac ctagttgctg gccagcctgg cttccaggaa 36420
ctggcctata gggccaggcc cctcccttgc ctctgattgg ataaagtggt gctgcactca 36480
cccctcactc agccctggag gctttgtgct ggtgtgtttt cctgggtttc agctgctctg 36540
ttgggagacc ctaatgtcct gatagactgg gaccccctgg ccctggaact tctggcctgc 36600
cctccagcat gcccctctgc agaacggcta catgctcttt ttttttcttt tttgtctttt 36660
tgccttttct agggctgctt cctgtggcat atggaggttc ccagactagt ggtctaattg 36720
gagctgcagc tgccggcctg tgccagaacc acagcaacgt gggatccaag ccgcatctgt 36780
gacctacacc acagctcatg gcaatgccgg atccttaacc caccgagcaa ggccaggggt 36840
tgatcccaca acctcatggt tcctagtcgg atttgttaac cactgcgcca cgatgggaac 36900
tcctacatac ccttcctgaa ggaaaggtcg aggaggctgg gaaaaacaag gcaggaagga 36960
acctggtctt ggaaccggca ggcaacaacc aggcctaaaa gtgcctggca ctgttgccag 37020
ctctgtcctg tatcataaaa tgttggagcc cgaagagccc tgcccgtcct cttcctttac 37080
aaaaggcaac accaagagca aacgccttga ggaagttgca catcaggtca gtggcagagc 37140
tggcgctctc cagagccctt gcagccccac tctgctagca agaggtgggg agtgctcgtt 37200
cttgtacatt tgtagcaccc tggggcctcg aggtgaaaac ccgggcctgt cacatctttg 37260
tattgccctt caggagaatc agaaagcctg gtgaatggga accacactcc acagcctgca 37320
acccggggac cctcagcctg tggcagccac agttccctgg tgagctctat tgagaaggac 37380
ctgcaggaaa tcatggactc actggtgcta gaggagcctg gagctgctgg caagaagcct 37440
gccgcaactt ccccactgtc accgatggcc aacggtggcc gctacctgct gtcccctcca 37500
accagccccg gcgccatgtc tgtgggctcc agctatgaga acacctctcc agccttctct 37560
ccactctcct caccagccag cagtggaagc tgtgccagcc actcgcccag tgggcaggag 37620
ccagcacctt ccatgccccc gctggtgcct gcccgttcct ccagctacca tttggccctg 37680
cagcccccac agtcccgacc cagcggtgct cgtccctccg agagcccccg cctgggcagg 37740
aagggggggc atgagaggcc gcccagccct ggcctccgag gtctcctgac agacagccct 37800
gcagccactg tcctggcaga ggcccgcaga gccacagaga gcccccggcc gggtgggcag 37860
ctgcccttgg tggcaatcag cctgagtgat tacccggctt ctggtgcccg cagccaaccc 37920
accagcattc ctggcagccc caagttccag cctccggtcc ctgctcccag aaacaagatt 37980
ggcaccctcc aggatcgccc tcccagcccc ttcagggagc cgccaggcac ggagcgggtg 38040
ttgaccacca gcccctcgcg ccagttggtg ggccgaacgt tttcggatgg gtcagccgcc 38100
cgcaccctgc agcctcctga gagcccccgc ctaggccggc ggggcctgga cagtatgaga 38160
gagctgcctc ccttgagccc atctctgtcc cgaagggctc tctcccccat gcccacccgg 38220
accaccccag atcccaaagt aaccagggaa gtggcggaca gtcctcggcc ccggcgctgg 38280
gcagcccatg gggcttcacc agaggacttc tccctgacgc tgggggcgcg gggccgcagg 38340
acacggagcc cttcccccac actcggggag tctctggcac cccgcaaggg cagcttcagt 38400
ggcaggctga gcccggctta cagtctgggc tctttgacgg gggcttcacc ccgccagagc 38460
ccccgtgccc agaggaagct ctccagcggg gacttgcggg tgcctgtgac tcgggagcgg 38520
aaaaatagca tcacggagat cagtgacaac gaggatgacc tcctggagta ccaccggcgg 38580
cagcgccaag agcggcttcg ggagcaggag atggagaggc tggtgagcgg atgccaggga 38640
ggctgccggc atccatagca ggggctctgc cagggtgtgg gcgagccagc tggcagggtg 38700
ggaggggccc tggatagggc ctgggcttcc caagcttctc tacttcccca gtacactgta 38760
gtgaccagta tctcaagcct ctggtgccct gaagaacttt ctttgcagtc tctaaggcct 38820
aatttaattt aatttaattt aatttttgct ttttagggct gcacctgcag catatggaag 38880
ttcccaggtt aggggtcaaa tcagaactac tgctgccagc ctaggccaca gccacagcaa 38940
caagagatcc aagctgcacc tacaccacag ttcatggcaa tgccaatcct taacccactg 39000
agtgaggcca gggatcaacc cacatcttca tggatactag tcaggttcgt tacctctgag 39060
ccacaacggg aactcctctg aggccttatt ttaaattcat gctaacttta catttattct 39120
ataaggcaat gtttatttta atgtgtaaat taagtttaca ttatttatct gaatacagtg 39180
aagctctaga aaaacacact acattcatat tgaggtcttg cacacctctc agtttgagaa 39240
acaggtctta atgactagaa gactgaagcc cagagagggg aatgagtttg tctgaggtca 39300
cacagcaagt tctggtcgag ctggaattgg aatccaagtt tctcgtcttt ccaaggctat 39360
tctttagaaa agcctacttt cttgcttctc ttggcctcta taaatggatg agagctgctc 39420
accgtggtct atgcaggatc atctgggaac gggggtggca ggggagagga aagaacagga 39480
tgggcagccc ctggctgatc taggctggcc tgtgtgattg taatggtgtg tgctggggtg 39540
tgtgcatgca cactctgcct gtgggtgcat gtgggggctg ggtgtggctc tgggcagctg 39600
tcctggagat ggcatctgag ctgggtgggg gctgggattc cccagatagc acagggagct 39660
ccggggctct ggctggtgta tacacactca agcaacatgg ataggaaaat agattctgct 39720
ctctctccct tgtgagcaga agagaggggt tctcgggggc ccagggacac tgggattggt 39780
ttgaggcgga ggaaggagaa tcagaactga agaagtttgt ctggacaccc ttgcagaaga 39840
ggttgggaca gctttaggtg aggtgagggt gtgggatagt gtgttagtgt tgggttgggg 39900
agggatgagg caggatccct cgtttttttc tgttgctgtt atcttctctt cactcttttg 39960
gcctctgctt agacaagaat gcttggccta ccctggggtc tcatcccaga gaggggacag 40020
tgaacctggc acagcagggt ggtgggccca gcctccctcg actcctcctc cgttcagcct 40080
tcccaccccc accctgctcc agccccacgt gtctcaccac cagctctgac ctgccccacc 40140
tgccacttta aatcttggag gctgcctgcc ctgcccctcc cacctctaca ggctgcttat 40200
ctgggtcccg gggcaggggc gggggcactg gcccagcctc ctgggttctg agactctgtt 40260
cctggttttc ttgacatgga actgggcaag cagcaggagg gaagctgggt atgtgtccgg 40320
gggcggggga gggggggtcc tggccaaatg attggtgaaa aacaccagat gttgagggac 40380
ccagaaggaa gagccgtggg agcttgggga gaggcgaccc agacctggag cgtgactgca 40440
ccgttcctcc ttcactttct aggccccctg agcggtatct gggtgggctg tgtgtgggga 40500
gggggcagat gaggcgacag ggccctggag cactgacacc tgggcattca aggtgggagg 40560
gaggtgaggg ctgcctgaga gtgacttcga attgagagga ggatggggaa gacctgcccc 40620
gggtctctgg ggcagggtgt gtgcacgctg aggacatctt cctgtgaacc tatgtcaggt 40680
cctgtggccc cgggtctgtt ttccagccct gtctgtgtcg gtgcccacct gtctgtacgc 40740
tagactgtgt ttatcggtgt gagtgtgcgc cagtgtgcat ttgtgcatgt caggactggc 40800
gtgtttgtgt gtgcctgcct aacaatgcgg gcccggggca cgtgttgtgt ccttgtgtgt 40860
gtcggtgctg gtatgtaggg gtgtctggat ctgtggattg gggtctgtgg gtctatgtgt 40920
atccgtgtca gtgtgagcac ctgcttgttt atctgtgtct caccgtggtc tttagtctgc 40980
ttaccagtgt gtgttcctgg atgtgtctgg atgtgtccac aaggaagccc accctgggac 41040
agaatgagaa gggaaaggaa acaggaagac aggaggggct ggccacgtgg acaggtggca 41100
caatagcttg gttcctcctg cctgcttctg ggacaccctc cccccttccc tctgcctccc 41160
tagactctgt gcttggggct ctcctgactt gatttggagg ccctggggtc ctagtcctct 41220
ctcctgacca gacactgtcc ccggctctag ccccacgggg acccgcctca tcccctccat 41280
gccctgctct gtccaccctc cccaggaacg ccagcgcctg gagaccatcc tgaacctgtg 41340
tgccgagtac agccgggccg acgggggacc tgaggccggg gagctgccca gcatcgggga 41400
ggctgcggca gccttggccc tggccggccg gaggccctca cgcggcctgg gggcggtcac 41460
tggggcctct gggcggagca gtgaggagcc tggaggtgcc acccagcgcc tgtgggagag 41520
tgtggagcgc tcggatgagg agaatctcaa ggaggagtgc agcagcactg agagcaccca 41580
gcaggaggtg ggactcgcag gtggccccag gaggcaggct gggtggtggg tgccaaggcc 41640
ccagcgagtg ggacataatt gcagttcggg acaacatcag ggatgagaag tgtagaaaag 41700
aagaggcaag gaagaggggg aggggtggct gagtttccca agctgtaaat atgctttatg 41760
acctgccttc ccttggggcc ccacccaggc ctggctctca gtggtgagtc tttattcctt 41820
tcagctgagc ccgaattgct ccccttgcag ggaggggaat tgcctcctat ctcagtccag 41880
ctgctccttc ccagcgccat tttcttagtc atcctggaag gctctaggct tccccagctg 41940
atggcttctc tcccctctag catgaagatg cacctagcac caagctccag ggagaggtgc 42000
tggccctgga agaggagcgg gctcaggtgc tgggacgagt ggagcagctc aaagtccgtg 42060
tgaaggagct ggagcagcag ctgcaggagt cggcccgaga ggtgagagcg agggacctgg 42120
ctggactcct tcccaggcac aattgacggc ctgcacagtg gccagagtct agctgccttg 42180
agtggccttg acatggccac tgctgttgcc tccaggctga aatggagcgg gcgttgctgc 42240
agggggagag ggaggcagag cgggcactgc tgcagaagga gcaaaaggcg gtggaccagc 42300
tgcaggagaa gctggtgacc ttggagacgg gcatccagaa ggagagggac aaggtaacac 42360
ctggcctggc tcagtgctgg gcaccagtgg cctgggagag aaggagaaag gccttctcta 42420
gggcccctta ccccttccct cacgttcatc ctgcagacca ggtggtctct gtgcccctcc 42480
aggggcaggg agccttgtcc tctgtggaaa ataccagctt gaagggtgct gccagggtct 42540
gggctggtgt ctggggtctc ctctgcctgg tctctgttct acctgtgggt gcctgggtgt 42600
ctgtcattct ggacccgtct aggaagtgcc gaagcccctt ctctggcggt gagcacagcc 42660
caagggactg gtgccgggga gtgactcagg tttgggcccc tgcatccgga ggtgtccccc 42720
acccccctgc ccaagcctgc ctctggggct tgggacatgg tctttgggag agggccctca 42780
tgtctttgat tccccttccg agtctgcagc ctcccatccc agatggtccc agctcctgcc 42840
cctgcccctt gcagatttcg tgccagcctg caggggccgc tccgtcccac tggggccttg 42900
ccagccatgt ccaagatgcc caacaacgtg ccaaaagctc tgaagtggag aggggccacc 42960
tatttctgga cttgacagta tttttccaag aattaatttt ccttctggat tttttgagag 43020
cgtcttcctc ctgcccctca attttgaagc tcaaaaattc tttgaccccc tggccttttt 43080
ttggagcttt ggttctggtc cccaaaacca cactcccaaa ttctgtgcca aattaactcc 43140
atgcttacca accaggcctg cactaacgcc cccctctaat cgctgtcctg ggctctccgc 43200
atgcccctaa ctgccagtgc cacccaggca ggcagggctg agccttgcct gcttttgcac 43260
taactccatg ggcacctctg tgcccttggc ctctcccctc taatgttgcc tccttccttt 43320
cttggtgtgg gaccccaggg ttttgatcag aaaggaatgg gcagggagca ctgctcaact 43380
ttacacagtg gggcaggagg tgcacaagac tgggggaaat gcttggctga ggagcttctc 43440
agcctgaagg gctcctctca cttggaaaag ccagggtgct gcctccacag gctgttggca 43500
gtttcctgca tagtggagag gcaggctgtc ctccagccat tgcatgcctt ccctcctgag 43560
cggaggagtg aggagttccc ttggtggtgg catggcctgg ggagggtgtg gacagggcct 43620
ccctgccaca cagtggctgc ccttcttagc tttcctctgg ggcttcctgg ttgcagatac 43680
tccacccaga gggtgggaag gggtaggtgt ctccccactc ccagccctga tccatcctga 43740
gcagagggta ttttgaaggc tgagtctgag catggggaag tgcttggctc ccaggagctg 43800
gcctgctcag gtccccctct ccaaggatgg gggtggaggg gtgggcagag gcaggggtca 43860
gcgtagacac gctgggtgaa gctcagcatt tccgaaggaa cctgcctgat ctcacttctc 43920
attgaccagg cattgcctgg agacttggac tttgccttct ttctgggctc agttatttga 43980
ggtggtggtg gtggtgatgg tggtggtggt ggtggtggtg gtggtggtgg tggtggtggt 44040
ggcagtggtg gggaaggttc cctcagcttc ccttcccagc ccccagccca tggccccact 44100
ctgacttggg aatcagtaat cagtttctcc tctgcctgtc cttcggaggg gtgactcacc 44160
agctcctccc ctcaccccct tcccgctcag gtctcttnnt aaaannnnnn nnnnnnnnnn 44220
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 44280
nnnnnnnnnn nnnnnnnnnn nnnntcttgg gccctacaga aactctctag aaggaatttt 44340
ggagggagca gggcagcatc tacttctaac tcctcccctc ctccctggag ccccccctgt 44400
cctcccccag gcaaacctag cctgtcctcc acccccaggc tgcccggccc agccctttac 44460
accacgtggc tgactgtgtt cactccctcg ctcccctgct ggctgggttc cctcttcccc 44520
gagctcctcg ggccaggcct ctgccactgc cgatcggggt ccatccctgg gctcagcaca 44580
gtctggtaca cactttctct ccttcctccg aggctcagag agggcagcgg cttgcccaag 44640
gctatacagc acccgacctc tggcctggcc cccagggccc tggcaccagc tggcatggag 44700
cagtgctcca ggctcctggg ccaacctggg ccacatcctc acccagcccc tcacccagtg 44760
ggaacgatct caagcccctt gattcccctt caaatcacgg acaggctgac ctgctcccca 44820
ctaaacccac ctcactggca tagaactggg aggggatgaa gcgagaacac acctgatgtg 44880
gacacagcca ccccctcggg gcctgcctgc tctccctcag cccattcacc acatctgcct 44940
cactggggaa aggtgctggg gctcagccac accccggcca gatatcctgg ggccctgatg 45000
gtggcagcag agacacgggg gcacagggag ccaggaaaat ggtcccccag ctgggtccta 45060
ataatggcca ctgcggggtg agcacctggg gtgtcaggct gggcggtggt ggctcctgca 45120
caccttcgtg ctcttgctgg ggctccagag gccagccggg gacacgagca gaccacgctc 45180
cccacggccc tggccttgac cgaggccaca cggcagagct gccccagagc cagaacccac 45240
agctgccagg gcctcccccg cggcctcctc ccgcctcccc tccacttggc cctgggctcc 45300
ccgcaccctc tctcttgact ccaggtgaag ccagaggtcc tcagccaccc cgagcggtcc 45360
tctctcatct actcgggtca cccgttcatc gtgccaggag gacgctttgt cgagttctat 45420
tactggtgag cggccaagag ctgcgggggt cggggggctg gggccagcgg ggcgtcccag 45480
gcggggtggg ggatcctgag ctgtccttcc cccagggact cctactgggt gatggagggt 45540
ctgctcctgt ccgagatgcc agggacggtg aagggcatgc tgcagaactt cctggacttg 45600
gtgcagacgt aagaggcccg ggcatctcag ccggggtgca ggaggggcag ggccctgggc 45660
ttgggccgag atctgcctgg gctcggggcg gggggtggtt gcagtggggg caggggtgtc 45720
agcggccccg ttctcggcag gtacggacac gtccccaacg gggcccgcgt gtactacctg 45780
cagcggagcc agcccccgct cctgagcctc atgatggagc gctacgtgac tcaggccaat 45840
gacaccgcct tcctccggtg gccaccccgc cccctgctgc cctcgccccc cttcctgagg 45900
ctccctgggg cggggcgggg cgggggctga gctggtgcct ggctccaccc cagggacaac 45960
ctggagaccc tggccttgga gttggacttt tggaccaaga acaggagcat ctctgtgagc 46020
tctgggggaa agagctacgt cctgaaccgc tatcatgtcc cctatggggg acccaggtga 46080
ggccgtgcct ggccagccct tcagtctctt gtgtgttcac tctggtttga gtggccagac 46140
tcccctggtg atggtgatac tgagggcctg ggggcctgtg ttcccaggaa aggggtccca 46200
actatgctct gggggcagcg tggcactggc tagatgccct gttgtgcatc acccctttct 46260
tggaggtcca gccctggctc tgggccatcg ctggggcagg agacacccta gggggccaag 46320
gaagcaggca ggaccccacc aggagttggg gcggagcccc agcaggggag atgtgaacag 46380
ctctgtctgc agaggcagtg gaggagcccc ctggggagga ctcccagagg tcagaccact 46440
gtggagcctg cttctttgca gcttggccac cagccaggct gggcaggaga gagggtctct 46500
cctctcgctg gactcgcatg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 46560
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 46620
naaggcaggg cctagagcaa gacaagggag cctcacagtc ccctccnccg gccctgctca 46680
aaccacagcc acctgtagat caaaaacaac ttttttttca accttcagtt gcctgaaaga 46740
tgtacatggt tggggctggg accgagagtc aagggcagcc tgtacacaca gggtaggggg 46800
aactcctggg cccctgctct cagactttgc acatttctgc atttctgcac atgacagcag 46860
tttttgcaga aagggtgtag agggtgcgtc tgggccaggc atcagcctgt ggcagctgcc 46920
tgtggttgcc tctgcccacc aggatgccca acctcttgtt ctcacctcaa ccaccatggc 46980
ttcctgtccc catgggaaca gggcactgag tcaggggctc ctggcacacc cagataactg 47040
ggtatctctc tgccaggcca ccctaccatt ccaggtcatc tgtatatcag ttgaagcctt 47100
cgctggccat taggggttgc aaatgcaaat tctaggacac aattgtggga catcttaagg 47160
aaactctgca agggggaatg accctgcctg gggactggta acaggcaagg gggatgactc 47220
tgcctgggga cattcctgtg taaccagggt ctccctgctg aagatggttg gtgtaaggga 47280
agatggggtc cctatttgct cgctcttcca gtttttcaat gtaaggtgga gatccatatt 47340
tttatgtgaa atatcccaat tttgaagggt ctcgatatcc caaattctgg ctgtccatct 47400
aggagtgatg cctgtgttgt cagtgaggat tgaggaggag acctgacccc actacagggc 47460
ccttcttctc cccacagcac ccagggccca gccctcaaac aagacctcaa gcctgcgagc 47520
tgactccccc agttgtgctg tgccctttgc ggtgaggtac tctgtacata ttgagatcct 47580
taatgtgtca agatctctta caaagaaaaa ataatattgt cattcttgaa aaaaaacccc 47640
atagactctc tatgggcagt cgataaatat aatgctctat tcattctttt tctgatctgg 47700
atcaggcctc tacccacaag gacacagctc ccttttgccc ttggggagtc tgtgcctgac 47760
cttggatgtt ctcacccaag tctcacccct tagcatttct gccttgtctt tctcagcaca 47820
tttacaagca ggtgcatggt tggtgggaaa aatgatactt taaaatattt attgagcttt 47880
gattttgcag tgaagctgca ggcttgtagg ggaaggactg acaaggggac ctgagagtca 47940
gcccagcctt agtaagtctg ctctgggctc aaatgtacac cctcacccac taaaagacct 48000
gctggaccac tcatccttgg aaggcagcct aagcgggtgg gagtcaatgg gaggggaagc 48060
aatctccttg cttggacccc aaagtttttc ttttcttttt ttcttttttc ttttttttta 48120
cggctgtacc tggggcatat tgaagtttgc aggctagggg tcgaatcgga gctgcagcct 48180
acaccacagc cacagcaatg ccagatctga gccatgtctg caacttatac cacagcttaa 48240
cccactgagc aaggccaggg gtcaaaccca catcctcacg gatactagtc aggctcataa 48300
cccactaagt cacaatggga atgacccaaa gtttttcttt gaggacgaac tactgatttc 48360
cttctcaatg tttattctca aggtagcagt gtgactaagc tggtacattt ccaggctccc 48420
ctgtagttag gggggcctgg ggacccagtc cttaaaaaca agatggaagt gggagccact 48480
ggaagaagtt cctgggaaag ctccttaaaa tgggaaaaac tcagtgggct ttgctcttgg 48540
tcctttgccc ttccccctgc ttcctgcctt gagcactgat gaaaggttta gaactgcaga 48600
agccaccctg cgaccacaag gcaatgaacc tgaggctgaa aaacacaggc tgaagcctat 48660
aggctgacgc tagcagggca ggaagatggg gagactggac cctcagtggt accattgagt 48720
cacaacagcc ctgggctccc cttatatgag aaaaaaaccc ccccgtcgct gtgtaagcta 48780
ctggcgttca gatttctatg cctttgggct gaaaggggtc tctatggttc tggttgggtt 48840
tgtaacctgc tgagccacaa cgggaactcc aagagtccac cattctgctt ggtttggcct 48900
attcggacag gagggaaaag gggtctgtga tggccacagg ctggacagtc gcagcaccag 48960
tctgaaaacc cagtgtggga cgctacccga gaagggcagg gctgccaggc attgacccat 49020
ctatggaaat ctaacgggaa tcattcatct cttagaatag ccgcacacct gctgagaatc 49080
agtttctatt gcggtgagaa gaagttaaaa aagcaaatga aagggcaggt gaagaacgag 49140
cgtttctcta acataccagc ctgtgccgca tcgggctcct gccactgtct gtcaccaccc 49200
acgtgcctcc ctcaatcagt gccgcccaca agtgtgagga cttgggcttt agggcgcctg 49260
tgtctaagcc tggagtctgc aacctgcagc tccaccccac cctccgggga tctgggagcc 49320
aagcctgcct ctgagggagg gtctcggagg cacggaccct ggtttgtcac atggtctcca 49380
taagtacagc agtcaggtga cctcttgggg cctctgtggc atcatttgta acgtggggct 49440
aatatgcccc ttggaggatc attataagga ttagagtttc gggtgtgcag agccgaccac 49500
gggcagcggc tgctcagtga ttgggtagag atgctctagt taagtccatc agggcagctg 49560
taaggctgtg ggaagcttgt tccctcagtg ccacgcaggt ctcatatgtg gatgaccaac 49620
atccctgagt tcaggagctc cacatgaggc tgctccttgc gggccctccc actctgagcc 49680
ttgtatgaca caaatctgtt gtcagaaggg ggtagcaggt gcccagctga gcatcattcc 49740
aagaaataac tggcgctggg acaagcgaag accagcctcc aggcagcaga agaggactat 49800
cctgggggcc tcgccacagt ctagctggag tgaagggggg ctgctccaag ccagcccccc 49860
acagaggcct gcagctcagt gcccagcagt ggagaacctg gaatggaaag cttccagatt 49920
cttcagcaag ggccacctgc agcacccaca gcccaggtgc acggcttgtt cgtagcttga 49980
ttcctcaggc tgtctctctg cctggggatt cctgcaagaa ggatgtggtt tgatcaacct 50040
ggagaggatg ggtagggaat gatctgccga aagttctagg ctctgctcca tgcttgggaa 50100
accctcccag gtgtagctgc tctcctgaat ttgcagtttc cccctgctgt gctgtcttct 50160
tacttttgct gtatctatct acaaactatg tgtagtattg ctttgcatat gattttcttt 50220
ttgttttttt tgctttttca gggccacacc tgtggcatat ggaagttccc aggttagggg 50280
tggaatcaga gctgccagcc ttcaccatag ccacaacaac tcaggatctg agccatgtct 50340
gtgacctata ctgcagctca cagcaatgct ggatctttaa cccactgagc aaggccgggg 50400
gtcaaaccca catcctcatg gatactagtt ggattcataa cccgctgtgc cacaagggaa 50460
ctctctgcat ttttttttaa attgtggtaa aacatcatat aaaatttacc atttaccatt 50520
ttgaccaatt tttattgtag gatcccgagg cactaagtac attcacattg ttgtgtaacc 50580
atcaccacca tgcgtctcta gaaatttttt atcttccata actgaaactc tgtgcccatt 50640
agtcaataac ttccctatcc ctcctccctc acaacccctg gaaaccacca ctacttttca 50700
tctttataga tttgtggttc ttcgtgtaaa tagaatcata cagtcataca gtattcgttc 50760
ttttgtgtct gattagcaga gtatcttcaa ggttcatcta tgctgtagca tgcatcagaa 50820
tttctttcct tttaaaggct gaatagggat tccctggtgg tcttagtggt ttaagatgtg 50880
gcattgttgc tgcttcagct tgggtgtgat ccctggccag ggaatttctg tatgctatgc 50940
gtgcagccaa aaaaaaaaaa aaaaaaaagg ctgaatgata ttccattgta tgtatatgcc 51000
atgtgtttct tattctctca tctgttggtg gacacttggg ttttttccaa ctttcggctc 51060
ttgtgaataa tgctgcagtg aacatgagtg tataaatatc tcttcaagta cttgtgttca 51120
gttctttggg ggatgtacac aggtagaatt gctggattat tttttatgat gatttttatt 51180
ttttccatta gagctggttt atagtgttct gtcaattttc tactatacag caaggtgact 51240
cagttacaga cttgctgggt tatatgttga ttctgtgttt aattgtttca agaagcctca 51300
tacttttgca tgttttaaaa tcttaggtac tatatatgga ggttattgtg ctgtatcatt 51360
tggcaatttg ctgctctttt tttttttttt tttttttttg gtgctggtat gattctgaga 51420
ttcagctgtg ttgatacact catctaatct gagaatccgt atcttttaac ttacaagttt 51480
aggtattgtc attctttgga tttattttta ccatcttatt ttgtgctcct taatttcctc 51540
atggttttta ctttttcatt tgttcccctt cccttgtttc ctgtaactgt ccaatttagc 51600
cagcctcttt tatttttttc tacttttatt ccttccttca tttgttttct tggaaattat 51660
cggtttcgtt cttattcttt aggacttctc tctccttttc atatgtacac tgactctaaa 51720
gttaatcaga aacatgattg tcctgtgatg gaggaagtgc ctatggaacc ctttaactca 51780
gatcgcctcc tctctcttct gtatcaccat ccaggcttgc ggacctgctt tagttttcac 51840
gcttccaact catcaccatc atggttgcag ctggggctta tgtccgttta cccacatgtt 51900
tacaatgtgc tcttcattgc ttttttcttt ttaattgaag tatagttaat ttacaatgta 51960
gtgttagttt caaagtgatt ccgttataca tatatatgta tatgtgtaca tatgcttttt 52020
cagattcttt tctaagttct tagaccatat tgagtatagt tccctatgct ataccgtagg 52080
tctttgttgt tgctcttcat tgcttttgac acctcatacc tcccgagttc agtttccttt 52140
ccctagaaat gaatgtgtta gcgactctaa caccagtgat attatggtga actctttccg 52200
tctgcacctg aaaaggtctt taatttcact ttcactcatg ctgttggatt gtcagcagct 52260
acagctctga ttagacccct agcctgggaa cctccatatg ctgcaagtgg ggccctaaaa 52320
agaccccccc ccaaaataat aataactgaa gccctcttga gtgatatttt aggagatttt 52380
aaactctagg gtgagctatt ttttttgtca gcactttgag tttagaatgc ctctgagttc 52440
catcttctgc tgctgctttt gagaagtctg ttatgagttg ttttcctttg taggtaatct 52500
gtcaggcgac ttaaaatcat caggttgttt ccactgttct gcagcttctc taaaacatat 52560
gtgacatagg cttctgtttc ctgctcaaat tgtacaactg ggtctgcctt ctcctttcac 52620
tcctaccgtt tctgagtgcc tactgtgtgc gaattgctat tctaagtgtt ggggattaaa 52680
cagtgaatgg aaaagaaaaa gactcttttc acagggaggc tctctctccc atttcagttg 52740
gagacagagc ctgagaacga agccagcatg cggaagagca gagctgaggt ttggagaaag 52800
cagatcctga gtgtctggcc tgatcctgcg ttaggcccca cgtaaaagca actgcgctct 52860
gtacgttttc actaaggtta acaaccgttg ccccttgttg cataagagtt ttaaatggat 52920
ttttatgcca tttacaagtg aaagggtacc tatacatgtc ccagcccagt gcgatccagc 52980
tgtccttcct ctgtgaaact ttacattcat atcttcattt gatgagttta acattcattc 53040
attcatttat tattgtttct agttggcctg ttcccaaaaa agatttaaga ccttttacaa 53100
agatagataa aacataaggt aacaatttag aaataagaat tactgtgctt attgaacctg 53160
aagagtcata tctttctctt ttttaaattt aattttgttg gagtatagtc ggcttccaat 53220
gttgtatttg tttccggtgt acagcaaagt gaatcagtca tacatatagg tatatccatt 53280
ctcttttacc atataggtta ttacaaacta ttgagtagat tttcctgtgc tacacagtag 53340
gttctcatta atcaactatt ttataccaaa gtatatgcgt tattgccacc ctcccaattc 53400
ttcccccccc cgccagcggt ttgaccagtc ataagactgg ttttgaaatc tgtgaatttg 53460
tttctgtttt ataagttctt ttgtatcttt tttttttttt tttgtctttt tttttttgcc 53520
ttttctaggg ctgctcccgc ggcatatgga ggttcccagg ctaggggtca aattggagct 53580
gtagccactg gcctacgcca gagccacagc aacgtgggat ccgagccacg tctgcaacct 53640
acaccatagg tcacagcaac gctggatcct taacccactg agcgaggcca gggatcaaac 53700
ctgcaacatc atggttccta gtcgaatttg ttaaccactg agccacgacg ggaactcctc 53760
ttttgtataa ttttttttta gattccacat ataagtgatg tcatatgata tttgtctttc 53820
tctctctgac atacttagtg tgataatatc taggtccatc cattattact gcaaatagca 53880
ttatttcatt attttttatg gctgaataat attcatatat ataaatatat ataaatatat 53940
ataaatatat aaaaatataa aaaaaatata tatatataaa aaaatatata tataccgtat 54000
cttctttatc catttctctg tctatgaaca gttaggttgt ttccatgtct tggctattgt 54060
aaatagtgct gcaatgaacc ttggagtgca tgtgtctttt tgaattatgg ttttctctgg 54120
atatatgccc aagagtgaga ttgctgcatc atatggtagt tctatattta gttttctgag 54180
gaatgtccat actgttttcc atagtgacag taccaattta cattcccacc aatttacatt 54240
cccttttttc tgtaccctct cccgcgttta ttgtttgtag agtttttgat gatggccatt 54300
ctgactgatg tgaagtgata cctcattgta gttttgactt gcattttctc taataattag 54360
tgatgttgag catcttttca cgtgtttctg gccatctatc tgtctttgga gaaatgtcta 54420
tttagatctg aagagtcaaa gctttcatta agtctgaaaa attctcaacc attatctctc 54480
gcaatagtac ctcagccatc ctctcttctg aaattcctat tacacgaacg ttggatccta 54540
tcatcttatt gtttgtgtca tttaacccct ctttcataat ttctatttct tttttttttt 54600
tgtctttttg tctttttttg ttgttgttgt tgttgctatt tcttgggccg ctcccgcggc 54660
atatggaggt tcccaggcta ggggtcaaat tggagctgta gccaccggcc tacgccagag 54720
ccacagcaac gcgggatccg aaccacgtct gcaacctaca ccacagctca cagcaatgcc 54780
ggatcgttaa cccactgagc aagggcaggg accgaacccg caacctcatg gttcctagtc 54840
ggattcatta accactgcgc catgacggga actcccataa tttctatttc ttctttctct 54900
gggtgatttt cttagatcag ttttccagtt cattattatc ctcttcattt atgttgaatt 54960
ttgagtttag cttatttatt gaattttaca tttgttacca tttttttcat ttctagaatt 55020
tctatttgat tcttattcat gtatgtctat tcatagcatt tcattctttt tttttttttt 55080
tttttttttt ttttttggct ttttgccttt tctagggctg ctcctgcggc atatggagat 55140
ttccaggcta ggggtctaat cggagctata gccgccggcc tacgccacag ccatagcaat 55200
gctggatcct taacctgctg agcaaggcca gggattgaac ccacaacctc atggttccta 55260
gtgggttcaa cctcatgctt tcgattcctt acatgtatct aggcatttta gtgatatcta 55320
ttctataata tccttagaat cttcatataa catctgaaat cttggcagtc taattttttt 55380
ttttttttga ctgttcttac agcatgcaga agttctgggg ccagggatcg aactcatgtc 55440
acagcagtga caacattgga tccttaactg ctaggccacc agggagcctt ggaggtttaa 55500
ttttgaggtt aagtttgctg actcttgttc ataatgatgg cctctttttt ctttctttct 55560
ctctctcttt tctttctttc tccctttctt ttttttggac ttttcagggc cttacccgcg 55620
gcatatggag gttcccagac taggggtcta atcggagctg taactgctgg cctgtgccac 55680
agccacagca acgcaggatc tgagccacgt ctgtgaccta caccacagct cacagcaatg 55740
ctggaccctt aacccactga gtgaggccag ggatcgaacc tgcaacctca tggttcctgt 55800
ttggatttgt ttctgctgcg ccacgacagg aactcccttt tctttttttt tttttttttg 55860
taattttgga tggcaagctt atgttcagtg ggactttata ttttcgaatc ccatgtgatc 55920
tggattgaaa gcatcgtatt tcagagttat tttgtgattg cttcttttag ggaccccaac 55980
agtatcacca actctgaaaa aaaaaattta tgttattctc ttgtcttgct gctggatccc 56040
atttatgtat gtatgtatgt attattatta ttattatttc ttaaatttcc ccaatacatt 56100
attttctttt ctactgtaca gcatggtgaa ccagttacac atacatgtat acattctttt 56160
ttctcgcatt atcatgctcc atcataagtg actagatgta gttcccagtg ctacacagca 56220
ggatctcatt gcttatccat tccagatgca atggtttgca tctattaacc ccaaattccc 56280
agtccatccc actccctccc cttccccttg gcaaccataa gtgtgttcac catatccatg 56340
agtttctttt ctgtggaaag gttcctttgt gccatgtatt agattccaga caaaagtgat 56400
aacatgtggt atttgtcttt ctctttctga ctgacttcac tcagtatgag agtgtctagt 56460
tccatccatg ttgctgcaag tggcattatt ttgttctttt ttatggctga gtagtatctg 56520
gatcccattt aaataaccat gaaggcggtg taaactcaag ccccaaacat gaggcacaga 56580
ctgtgtttag aaattctaaa aaggagaatt tccttttcca cccagagccc aggctgagac 56640
acacttgttt gctttgttcc tgggctaggg ataagatgct tctctagatt ctctttccac 56700
tgaggctgta actctctgaa gattctggat ctaggtggga actcagctcc acctttccac 56760
ttcacagctc gtgggtagtg ttgattacta ccctgcaggg cagctttgtt tctggtcctt 56820
ggtgagttct cctttctcag gaaatcaggt atacatttaa aaaaggatcc ttgcacttat 56880
atgtggaatc taaaaacaga accaaaaaac tcactgtgag ataatagaaa atttatatat 56940
cagtctctgc ccctagttcc tggtacatgg cccctaaaac ctttgtgatt tcctaagtgt 57000
taagaacacc agcagcatct tctcttctat tgggtgcctc tgaatgggct cctggaggcg 57060
gctggatggg agctggtcat cagaaagacc aatcaatgat tagaagcttg gaattctcag 57120
cccaccctca tccttcagga aaaatagagg ggctggacat ggagtgaata attgatcatg 57180
cctacatgag gaagcctcca taaaaaatcc cacttgtatg gggtttggag agctgcttct 57240
tcttcttctt cttttttttt tttttgtctt tttgccattt cttgggctgc tcccgtggca 57300
tatggaggtc cccaggctag gggttgaacc ggagctgtag ctgctggcct acaccagagc 57360
cacagcaatg cgggatccaa gccacgtctg tgacctacac cacagctcac ggcaacgcca 57420
gatcctcaac ccgccaggga tggaacccgc aacctcatgg tttctagtcg gatttgttaa 57480
ccactgagcc acgacgggaa ctcctggaga gcttccaggt ggtgaacaca tagacaccag 57540
gaggttgacc atcccagttc catgtggaca aatctcctgt actcaggacc ctcccagacc 57600
ttgccctctg catctcttca tctggctgtt catctgtagt ctttttcata tcctttaata 57660
agctggtaaa tgtttccctg agttctgtga gcccctttca caaattaata gaacccaagc 57720
agaagggaac ctcagatctc tagccagttg gctggaagta ctggtgtctg aagtgtgtgt 57780
gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgcggggga ggcagtcttg 57840
tgggactgag cccttaacct gtgggatttg atgctctctc tggctagaga gtgtcagaat 57900
tgaattaaat ggtaggacac cagtgtcaga gaaaatttct ccacacattt gatgaccaga 57960
agtgtcagaa gtggtgtttt ctgtgaaagt aaagcagaca cacagtggaa gacaaatacc 58020
taggaaggaa aaatctgaat ttttccctac actcataaac acagagaata ggttggtagt 58080
tttgagagat ggagggtggg tggcgggcaa aatgaatgaa aggggtaaaa aagtaaaaac 58140
tttcagttat aaaataaatg aagtcacggt gatgcaatgt atagctcagc aactgtagtt 58200
aataatactc tatattttga gttcccactg tggagcattg agttaagaac ccaattgtag 58260
cagcaagggt tgccatggag gaagtgggtt tgatccccag cccagcccag tgggttaaag 58320
gattcggcat tgctgcagct gtggcatagg tctcagctgc agcttggatt caatctctgg 58380
gctggaaact tccatatgcc aagggtgtgg ccataaaaaa aatgtatatt tgaaagtcgc 58440
taagagcata gatgctaagt ttctcatcac aagaagaaaa gttgtgacta tgtagggtaa 58500
tggacattaa ctagacttac tgtgatgatc atttcccaaa tacacatacc tggattcatt 58560
atgttgtacg cctgaaacta ataatgctgt aggtcaggtg tatctcagaa aaaaagacct 58620
ttcttacatt ttatgaccat tttaggcaat tgttgtagga gggttcttga tattttcaga 58680
aacagaagtc tggggggggt taaccccaat ttgtctagat tccattttct ttttaaaaaa 58740
tgaatgaaaa atattaatgt catgtgttgt atattcttta aaaataactt cattgtagga 58800
aatttagaaa aatttaaaca aaatgaaaat atgctagaat actaccatct tggggtaatg 58860
ttttggcact tgttctttca gacgttttcc taagcagata tcaagatctc ataagtcaaa 58920
ccttcccttc ttccacccct gtgggcaacc ataactctct tctccatgtc tgtgagtctg 58980
tttctgttct gtgagtaggt tcacttgtgt catattttag attccacatg taagtgatat 59040
gatatttgtc tttctctttc tgacttactt catttagttt gagactctct aggtccatcc 59100
atgttgctac aaatgacctt tcattctttt tatggctaag taatattcca cttcatataa 59160
tttataattt atatatatat atatatatca catcttctta atccacgcat ctgtcagtga 59220
acatttaggt tgcttccatg tgttggctac gtaaatagtg ctgctataag cattggggta 59280
catgtatctt tttgaattat agttctgtct ggacatgtgc ccaggagggg ggttgctggc 59340
tcatacagca actctacttt tagggttttt gtgtgtgtgt ctttttttag ggctgcacct 59400
gcagcatgtg gaagttctca ggctaagggt ctaatcagag ctgtagctgc tggcctacac 59460
catccctcac ggcaacgcca gatccttaac ccgctgagcg aggccaggga tggaacccat 59520
gtccccatgc atactagtca ggtttgttac ctctgagtta caaagggaag tccttagttt 59580
tttaagaaaa ctccagactg ttttccgtag tggctgcacc aatttacgtt cctactaaca 59640
gtgtaagagg tttccctttt ctccacaccc actcctgcac aaaatgttat agtttttggt 59700
acaaataact tttctttttt tatcttttgc tttttagggc cacatccacg gcgtatggag 59760
gtttccaggc taggggtcca attggagctt cagctgccgg cctacaccac agctcacagc 59820
aacgccagat acttaaccca ctgagtgagg ccagggatca aacccacaac ctcatggttc 59880
ctagtcagat tcgtttccgt tgtgccatga cgggaactcc caaatgatat tccttttgaa 59940
tgacagactg aggtctttgc ccctggcatt tgtgtagcaa ggaacaactc tcattccata 60000
aagcaaagca gcagcagtta gaggtggcag tggctgttgt gaggcactca tgctcacttg 60060
gaagtgagta gtgctacact ctggaaagtg tgtgcttgta gtcgacgacc tgccccgtag 60120
aagctgggga agagtctgtg tctacagtct cacacgttcc tgtgtcttga ttgtgggtga 60180
agctgagagc tctcatgtgg ctgcagctgt tccactgtcc ccagcacagc tcacatctgc 60240
ccgaggcccc tggtttgttt gtactcatcc agaatgctcg gggcttctgg gtgtgagctg 60300
gcttctaatg tgcctcagac agcctttcac ctgccccgga gaggggacca aatataatag 60360
cttctcggca gggtggatgc ccaggattta tgggacctga agcttaaaca ctttggagtg 60420
ctgctttaag aaaaagaatc aaattatgac cacaaagcta gatgcagaga tgctgagggg 60480
ccctagggct gacgtattac tgacttgaca ggaaatccgc ccctgctggc ctctagattt 60540
gccctgccta gaggccaatt ctgtttattt ccaaacatct taaattagat ttgtttcctt 60600
cttgcctttt gtaggcatat taagattaag aattgagcag acctgccata aaataatcat 60660
ttggggcaaa atttctctac tttaattaag ccttccctac tcaaatcact aacatgcttg 60720
agagaaacac aatactctta aaattgttga ggttattttt gtgccccagt atgtggtcaa 60780
tcctagagaa tgttccatgt gcacttgaaa agaatgtcta gtctggagtt cctgttgcag 60840
cgcagcagaa acaaatctga ctagtatcca tgaagatgtt agtttgatcc ctggactcgc 60900
tcagtgagtc agggatcccg tgttgccgtg agctgtgatg tagttcgcag atgaggctcg 60960
gggcctgtgt tgctatagct gtggcgtagt ttggcagctg tagctccaat tcaaccccta 61020
gcctgggaac ttccatatgc cgtgggtgtg gccctaaaaa aaaaaaagaa tgtatatttt 61080
gttttgtttt tttctttttg gatgtaatgt cttgaaaata tcaatgaagt ctaacatcta 61140
tggtgtcatt taggatcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 61200
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaat 61260
catattgtaa aatggccata ctacccaaag caatctacag attcaatgca atccctatca 61320
aattacccat gacatttttc acagaactag aacaaataat ccaaacattt atatggaaca 61380
gcaaaagacc cagaattgcc aaaacagtcc tgagaaacaa aaaacaagca ggaagcataa 61440
ctttcccaga cttcaagaaa tattacaaag ccacagtcat caaaacagtg tggtactagt 61500
attaaaacag acagacagac caatggaaca gaatagagaa cccagaaata aaccctgaca 61560
cctatggtca attaatcttt gacaaggaag gcaagaacat aaaatgggaa aaagaaagtc 61620
tattcagcaa gcattgctgg gaaacctgga cagctgcatg caaagcaatg aaattaaaac 61680
atactctcac accatgcaca aaaataaact caaaatggct gaaagactta aatataagtc 61740
aggacaccat caaactccta gaagaaaaca taggcaaaac actctctgac atcaacatca 61800
tgaatatttt ctcaggtcag tctcccaaag caatagaaat tggagcaaaa ataaaccaat 61860
gggacctaat caaactgaaa agcttttgca cagcaaaaga aaacaaaaag acaacttaca 61920
gaatgggaga aaatagtttc aaatgataca acagacaagg gcttaatctc tagaatatat 61980
aagcaattta tacaactcaa cagcaaaaaa gccaatcaac caatggaaaa atgggcaaaa 62040
gacctgaata gactgttctc caaggaagat atacagatgg ccagcaaaca catgaaaaaa 62100
tgctcaacat cactgattat aagagaaatg caaatcaaaa ctaccatgag atgccacctc 62160
acaccagtca gaatggaggt cattaataag tccacaaata acaagtgctg gaggggatgt 62220
ggagaaaaga gaaccctcct gcactgttgg tgggaatgta aactgctaca gccactatgg 62280
aaaacagttt ggagatacct tagaaatcta tacatagaac ttccatatga tcccacaatc 62340
ccactcttgg gcatatatcc agacaaaact ctacttaaaa gagacacatg cacccacatg 62400
ttcattgcag cactattcac aatagccagg acatagaaac aacccaaatt ggatttggaa 62460
gatgtggtat atatacacaa tggaatacta ctcagccata aaaaagaatg acataatgcc 62520
atttgcagca acatggatga aacgagactc tcatactgag tgaaatgagt cagaaagaca 62580
aagacaaata ctgtatgata tcacttataa ctggaatcta atatcaaatg aacatctcca 62640
cagaaaagaa aatcatggac ttggagaaca gacttgtggc tgcccgatga gagagggagg 62700
gagtgggagg gatcgggagc ttgaggttat cagatacaac ttagaataga tttacaagga 62760
gatcctgctg agtagcattg agaactatgt ctagatactc atactgcaac agaacaaagg 62820
gtggggaaaa gaatgtatac atgtaagaat aacttgaccc ccatgctgta cagcaggaaa 62880
aaaataaaat aaaaacacat agctatcgtt aaaaaaaaaa aaatatatat atatatatat 62940
atatatatat atatatatat agtcttgctg tgggctgtgg tagaggccag cagctatagc 63000
tccaattcag cccctagcct gggaacttcc atatgccaag ggtggggccc taaaaaacaa 63060
aaaagaaaaa aaaaagcaga aacttcaggg agttcctgtt gtgactcagt actaatgaat 63120
ccaactagta tccatgagga tgcaggtttg atccctggcc ctgttcagtg ggttaaggat 63180
ccagtgtagc catgatctgt ggcgtaggtc acaaatgcag ctcagatcct gcgttgctgt 63240
ggctgtggcg tagactggtg gctgtagctc caattcgacc tgtagcctgg gaacttcgat 63300
atgctgcagg tgtagcccta aaaaggaggg gaaaaaagca gaaattgcca agtgaccatt 63360
atgaatgtat ttaaagaact aaagtaaatt ttgcttaaag aaataaagga catgatagtg 63420
ttgcatccaa taaagaatat ccataaaaga gatgataaag agatggaaat tattttctta 63480
aaatcaaata agggtttcca gttttgagtc cagcacgtaa agagcttgga aggcatcact 63540
ctgttctaac aaaaatttaa aagctgaaca aatcgaagat taacgcctct tagatctttc 63600
tgagaactga ggtaacaggg caaactgtga tcctcaaaat tgagagacag acagggagat 63660
agagacatta cagcttacca gcaaaaactc acatgccaaa acctctaaag gaaataatac 63720
taggcttctt ttgcccagtt gtctagcttt caacaaaaaa ttacaaagca tactatttat 63780
ttatttattt agcctcacct gcattcatgt ggaagttcct gggccaggaa tcgatccaag 63840
ctgctgcagt gtcagtgcca tatccttaac ccactgtgcc ataagggtta actctcaagg 63900
caatattaaa agaccaaaaa gaagaaaaaa cagctcgaag agacagacaa agcatgagaa 63960
caagatttca gatatagcgg ggatgttgga attattagat caggaatttt agaacagata 64020
tgattaatat gctttttttt tttttttttg gctttttagg gctgcgcctg tggcatatgg 64080
aggttcccaa gcttggggga aaattggagc tttagccacc ggcctacgcc acagccatag 64140
caacaccaga tccttaaccc attgagcgag gccaggaatc gaacccagaa cctcatggtt 64200
cctagtcgga ttcgtttcct cggcgccatg aactccatga ttaatatgct acagtggaaa 64260
aattttaaaa catgcaagaa cagatgggtt tagaaagaat aaaaaagaaa tgctacatac 64320
aaaaaacatt gtaacagaaa tgaaaactcc ttttgatgga cccttcagta gacccgatac 64380
acctgaagaa agaacccatc agcttgagga tatgtcagta gaacttccaa acctcaaaaa 64440
caaagagaaa aaagacggaa acagaacaaa atatctaaga attgtgggac aagtacaaaa 64500
ggtgtaatat atgtgttaat agagctgtca gaaggagaag aaaaagagaa aggaacagaa 64560
ataatatttg aaacgataat gacagataat tgccccaaat taacatcaga caccaaatca 64620
cggatctaag aagctcagag aacacccagg agaataattg cccccaaatc tacaccctga 64680
catatcaaat caactgtgaa aagtcaaaca taaaagaaaa aaatctttaa taaagacagg 64740
ggaaaagaca ccctacctgt aggggactaa ggataagaat tgacttctcc tcagaaccaa 64800
tgtgagcgag aagagagcag gacaaagtat ttagtgttga gggaaataag tggatgtatg 64860
aacaaaatga gaatgttgag agagagaaac tgtgtatgga ggttcctcaa aaaacgaaaa 64920
atagaactac cacaggatcc agcaatccca cttctgggca tctatctgga gaaaaccatg 64980
atgtaaaaag atacatgcag cccagggttc acttcagcac catttacaat aaccaaaaca 65040
tggaagcaac cgaaaggtcc atcgacagag gattggataa agatgtggta caagagaata 65100
ttcctcagcc atagaaagaa tgaaataatg ccatttgcac tgacatggat ggacctacag 65160
agtatcagac taagtgaagt tagtcaggca atgatagaca aacacaatat gctaccactt 65220
atatgtggaa tcttaaaaaa aaaaaaggat aaaaaataaa cttattcata gaacagaaac 65280
agactcacag gcttcgaaaa acttatggtt accaaagggg acagctgggg aagagaggga 65340
tggactgggg gtttgggact ggcacatgca cactgagctc tatggaatga ttggccaaca 65400
gggacctgct gtagagcaca gagaacccta cccaatattc tgtgaccatc tatgtgggaa 65460
aagagtctga aagagaatgg atgtgtttac atgtaaaatt gaatcacttt gttgtgcaga 65520
aatgatcaca atattataaa tcagggagtt cccgtcgtgg cgcagtggtt aacgaatccg 65580
actaggaacc atgaggttgc gggttcggtc cctgcccttg ctcagtgggt taacgatccg 65640
gcgttgccgt gagctgtggt gtaggttgca gacgcggctc ggatcccgag ttgctgtggc 65700
tctgacgtag gccggtggct acagctccga ttagacccct agcctgggaa cctccatatg 65760
ccgcgggagc ggcccaagaa atggcaaaaa gacaaaaaaa aaaagaaaaa acaatattgt 65820
aaatcaatat acatcaataa aactttttga tgatggttgt acgctataag taaataaaat 65880
tcattgaatt aacaataaaa ctttaaaaaa ttaagttaaa aaaagagaga gagacagaaa 65940
ttaccagaag aaatcaagta gattgtagat tttggagctt aaaggtacaa tagtagctga 66000
aaggaaaaaa tttaccggag ggcttcaaca gcagatttga gaaagcaaaa ggttcagaga 66060
acttaaagat gggacaactg acattatcaa gtctgatgag caactcacca gggacaacat 66120
taactgccaa gttcgcctca gaaaccatgg tgaccagaac acaacgagat gactattgaa 66180
agcactgaaa gaaaaaaaag tcgatcaaga attctatgtc caggagttcc tattgcagcg 66240
tagtggaaac aaatccgact aaaaaccatg aggttgtggg ttcaatccct gttgctcagt 66300
gggttaagga tccggcgttg ccatgaactg tggtgtagat tgcagatgca gctcagatct 66360
ggcatggctg tggctgtggc tgtgggcggc agctgtagct ctgattggac ccctagcctg 66420
gggacctcca tatgccaggg gtgtggccgt ataaagcaaa aaaaaaaaaa aaaaaaaaat 66480
tcgatgtcca accaaactgt aattcaaaaa gaggaaattt aagaaactca cagatcagca 66540
aaagatgagg gaggttttac cactagctct gccccgcaag agatgctaaa aggagttctt 66600
tcatgttgaa atgacactat acagtaactc aacccctgta aagaaataaa gatctcctgt 66660
aaaagaaaac acatgggcaa atataaaagc cagtaccatt gcattttggg tttataacgc 66720
ccctttttaa taccattaga gaatttaaaa gacaaatgta aaaaaataaa tatcaattta 66780
tgttattggg cacacatagg ctgttagtga caacaatagc ataaggggga aagacagaca 66840
tgtattgtga tgtattggag cagaattttt gtattctgct gaagttaagt tggtattagt 66900
ttgaactagc ttgttataaa ttcaggatgt taatttagtt aattcatggt aactaaaaaa 66960
taaacccaaa aggaaatgag aagggaatca aaatgattca ctacaacaaa atcaactata 67020
cacaagagaa ggcagtaatg gaaaaaatga gggacaaaaa tagtataaga cggaagtttc 67080
ctggtgcgct agctgttaag aattctgcgt tatcactcct gtgactcggc tttgaccctc 67140
agcccaggaa cttctgcatg atgtgagcat agccaaaaga aaaacagtaa aagacgtgca 67200
agaagaaatt agcaaaatgg cagaagtaag attacactct ccagttgaaa gacagagagt 67260
gggagttcct gtcttggctc agcggttaac aaacccgact agtatccgta aggatgtggg 67320
ttcaatacct ggcctcgctc aataggttaa ggatctggca ttgccatgag ctgtagtgta 67380
ggtcacagac gtggctcaga tcctacattg ctgtggctgt ggcgtaggcc agtagccgta 67440
gctctgattg gacccctagc ctgggaacct ccatatgctg ctggtgaggc cctaaaaagc 67500
aaaaaaaaaa aaaaaaaaaa aagaagaaga aaaagaattt tttccccagg atgacagcct 67560
ccaggggagc tcacactaat gattattccc tgagaccaca gcctctatct agtgtcctgt 67620
ccccacagca aactacagcc accccctact ttcccaggac accctccaag acccacagtt 67680
gagcatggga agtagaatga ggagagctta agttgtctct gaaaccagac ctgatcccca 67740
ggttcttagc tctgggtgct gctaatggag gcgggagaac ttgggatttt gatgctggtt 67800
ttggtgacat ccatcatcag ctgaaggtac tttgcatcaa ggtttcccca agtttcgtaa 67860
tgtcaaaaga agatcttggc atactggctg gatgcaaatg gaacatacct gatgggatgt 67920
ccgtggcatt cattgtgaga tgggcaaagg gttgagtctc aggctttttc cttctggtca 67980
gatcttacca tgaactttct gctgtgactg gagatgagag aagacaggct agcccaaaga 68040
atggtgaccc taggggtatt ctaatatcca tatagatcca tgcttacatt ttttctgctc 68100
tgatgtgttg tgatttaaca atatgttgca tttcctgaaa taaaaagaaa aaagagtctc 68160
aaaagaagag aaaaaaaaaa aaaagacaga gttccctggt ggtctagtgg ctaaggattt 68220
ggcattgtca ctgctgtggc acaggtttga ttctaggcct aagaacttct gcatgccaca 68280
tgtatggcct aaaataaaat aaagtagatc ttaagtcaat aaactaactt tacacctaga 68340
ggggaactag aagagcaaac taaataaaaa gctagaagag aaagtaaata atgaagactg 68400
gaacatagat aaataaaata gaaaatggaa aatcaataga caaaatcaat aaagccaaaa 68460
ttggttcttt gaaacaacaa caataaaact gacaaatctt tagctaggaa aaaaaattga 68520
caaagaaaaa aagacagaag aggagttctc ttgtggcatg gcaggttaag gatccagtgt 68580
tgtcactgca gtggcttagt ttgctgctat ggcacaggtt cgatccctgg cctgggaact 68640
tccacatgct ggaggcacag acagaaatat atatatatat atattttata tataatatat 68700
atatatatat atatatatag gaaaaagaaa aagacagaag acacaaatct ctaaaatcaa 68760
aaatgaaaat ggggacatta ctacagaact taataaagat aatattagta tccatgagga 68820
tacgggttca acaatcacat gccaacaaat aagataacct agatgaaatg cacaaattct 68880
agaaacacat aaatttccta aactgatgca agaagaaata gaacatctca acagatttat 68940
aacaagtaag attataatta aaaaccctcc aacctataaa tccccatgtg ggagttaatt 69000
ttatgtgtca tattggcttg gccaagatac cttgatattt agtcaaagat tattctagat 69060
gtttctgtga aagaattttt tagatgagat taacctttaa atcagtagac tgagcaaact 69120
accctccatg atgtgagtgg gcctcaccca atcagttgaa gggcttagta gcaaaagatt 69180
gacctcctta gtagagggaa ctctgccaac agactgccta tagacttgaa ctgcaactct 69240
ttcctggatc tccagcctgc tggcctatct tgcagatttt ggacttgcca acccccaaag 69300
tcatgtaagt aaattcctca aaataagtcc cttcttcacc accacccacc ccacacatac 69360
acatcctggt gcttctgttt ctctggagaa ctttaatata atccaagaac aattccttca 69420
gtggtgaact ctaccatgca tttaaagaag aattaacacc agtctttctc tattttttcc 69480
aaaataggaa agaaaaaaac acttcctaac tcattctgta agaccagcat tacctgatgc 69540
ttaagtcaaa gaaagacatc acaagaaaat tacaatccaa tatcttttat tcacatgatg 69600
caaaaatcct cagcaaaata ctaataaaac aaatccagga gttcccgtcg tggcgcagtg 69660
gttaacgaat ccgactagga accatgaggt tgcgggttcg atccctgccc ttgctcagtg 69720
ggttaacgat ccggcgttgc catgagctgt ggtgtaggtt gcagacgtgg ctcggatcct 69780
gcgttgctgt ggctctggcg taggctggtg gctacagctc cgattcaacc cctagcctgg 69840
gaacctccat atgccgcggg agcggcccaa gaaatagcaa caacaacaac aacaaaagac 69900
aaaagacaaa aaaaaaaaac ccaaaaaaaa aaaaaaaaac aaaaaaaaca aatccaaaag 69960
tatatttaaa ggattataca ccatacaagt cagttttatc ccaagaatgt aaggatagtt 70020
caatataaaa aattaatcaa tgttggagtt cccgtcgtgg tgcagtggaa acgaaactga 70080
ctaggaacca tgaggttgcg ggttcaatgc ctggcctcac tcagtggctt aaagatccag 70140
ggttgctgtg agatgtggta taggtcgcag acgtggcttg gatctagcgt tgctgtggct 70200
ctggcgtagg ccagcagcaa ctgctccaat tggaacctta gcctgggaac ctccatatgc 70260
cactggtgcg gccctcaaaa gacaaaaaaa aaaaaaaact aatcaatgta atacactaca 70320
ttaatagtac aaagaaaaaa acgcacatgt cattaattga cagagaaaag ccatttgaga 70380
gaaaagccat gtgagagata agccatttga ccatgattct aaatatagaa aatcccttct 70440
gtggtttaaa aaaaaaaaat cctaggagtt cctgttgtga ctcagcaagt tatgaacccg 70500
acgagtatct atgaggttgc aggttcaatc cctggcccca gtcaatgggt taaagatcct 70560
acatcaccat gagttgtggt ataggtcgca gattcagctc tgatcctgct ttgctgtggc 70620
tgtggtatag gccagtagct gcagctctga ttagacctct agcctgggaa cttctatagg 70680
ctatgggtgt ggccctaaaa aggggggggg gcaaacccta gaaactagga atagaatgaa 70740
atttcctcaa tatataggga atttatgaaa agcccacatc tagaatcata ctcaaaggtg 70800
aaagactgaa agctttctcc ccaaaatcag gggggaaaaa caagaatgtc tcctttcacc 70860
cctgtgattc aacactattc cagagttcca gctagagcaa caggagaaag aaagaaagag 70920
aggaagggag gaaggaggga gggaggtagg gagggagaga gaaagaggga gggagagaga 70980
gggggaggga ggaaggaagg gaggaaggga ggaagaggag gaaagaaaag aaaaagaaaa 71040
agaaataaaa ggcactcaaa ttggaaagga agactatatc tatccacaaa tgacatgatt 71100
ctatatatag gaaatcccaa aggacccaca ggaaagctac tagatctaat aaacagattc 71160
agtaaagctt cagggtacaa aataacacac aaaaattggt tgagttgcta tacacctgca 71220
aagaacaaac tgaaaaagaa atttaaaaag caatccagga gttcccatgg tggagcaatg 71280
gattaagaat ccaactatag tggctggggt cgctgtggag gcgtggattc aatccccagc 71340
acagcgcagt gggttaaagg atctggcatt gccacagctg cagcataggt cacagctaca 71400
gctcagaatc agtccccagc ctgggagctt ccacatgctg tgggtacggc cttttaaaaa 71460
aattaaataa aaataaaaag gaatgccatt taaaatagca tctaaaagaa taaaatacct 71520
aggaataaat ttattcaagg agatgaacga catacactga acactaaaaa cattactgca 71580
agaaattaaa taagagctaa ataaaagcga tggcatccag tgtttgcggg taagaaggct 71640
taatattgtt aagatgtcat aatacaaaaa gcaatctaca aattcaacac aatccttatc 71700
aaaattccaa cagccttttt tggagaaagg gaaaagctga tctaatttgt atggaattgc 71760
aagacaccct gcataaccaa aaccatctca gaaaagaaca aagtcagaga gctcacactt 71820
cctgatttca aaacttgcca caaattttta aacgggcaaa aaccttgagt aaatgtttct 71880
ctgaagaaga tatgcaaaca gccaatgagc ataaaagatg ctcaacccca gtaattatta 71940
ggaaaatgca agtcaaaacc accatgagat gttatctcac aatcactagc atggctgtta 72000
caagaaaaac caacaaaata acgagagctg ggagaaattg caaccattgt gcatcggtgg 72060
tgggaatgta aaatggttca cctgctaagg aaaacagcct ggcatttcct caaacattaa 72120
acacagattg accatatggt ccagcaattc cattcctagg tacatacccc ccaaaaatga 72180
aagcagggag aggaacagat attgtccccc cattttcata gcagcataat ttgcaaagcc 72240
aaacggtgaa ggcaacccac atgtccaatg ataaatgaaa ggatgaagta aatgcagtct 72300
tggagttccc atcctggcgc agtagaaacg aatccgacta agaaccatga ggttgtgggt 72360
ttgaatcctg gcctcgttca gtgggttaag gatccggcgt tgccgtgagc tgtggtgtag 72420
gtcgcagacg cggctcggat ctggcgtggc tgtggctgtg gtgtaggccg gcagctgtag 72480
ctctcatcgg acctctagcc tgggaacctc catatgccgc aggtgcagcc gtaaaaagca 72540
aaataaataa ataaataaat aaaaagaaat ttaaaaaaaa agaaaatgtg gtctctacat 72600
acaatagaat attattcagt ctcagaaaag aaaggaaatt ctgatacatg ctaaaacgta 72660
gatgaacttt gaaaacatta tgctggtgaa atgaggaaga cgcagaagga cgaatacggt 72720
atggctccac ttacatgaag tacctagaat aggcaaattc atagacacaa aactagaatc 72780
atgttttcca ggatccatgg aaaaaaaggg attaagaagt cattgcttaa tgttttttgc 72840
tttttcattt ttggccgccc tgaaacatgt ggagttccca ggccaggggt cagatccgaa 72900
tcacagtcat gaggcagcag ccgggatgga acctgaaact cagcaatccc aagatgccac 72960
ccatttcatt gtgccacagt gggaacttct gctcaatggt ttaaagtatg tttgggggag 73020
ttcctgtcgt ggcgcagtgg ttaacgaatc cgactaggaa ccatgaggtt gtgggttcga 73080
tccctgccct tgctcagtgg gttaaggatc tggtgttgcc gtgagctgtg gtgtaggtag 73140
cagacgcggc tcggatcccg agttgctgtg gctctggtgt aggctggcag ctacagctcc 73200
gattagaccc ctagcctggg aacccccata tgccgaggga gcggcccaag aaatgcaaaa 73260
aaaaaaaaga caaaaaaata aataaataaa gtatgtttgg gatgataaaa acaaaaaggg 73320
ttggaaataa ataatggcga cagttgcaac attgtgaatg taatgtaaat tgaactgtgc 73380
gcttaaaaat ggttgaaatg gctaatttta cgttatatta accacaagaa aaaacatttt 73440
aaaaaggaag gaaggaaaca taaagcatca gtagggaagg gtggcaggaa ggaggtgggc 73500
agacagagca gatgcccgct ccagtggggg cagagcactg gctgcaaaag cacagggcct 73560
ggcccacccc ctcgtgtgca gacactgagc aggcacccaa cagacagcct ggacagcaag 73620
cctgagtcac acaggaaagg tcacacggtc accacacgcc gatgaccaag gctgacacag 73680
gcaccttctc ccacccacct cctgggctgg ttcagcctga atcactgacc ctggatcatc 73740
actacagcct cctggtgtgc cctgccccac ctctgcccca aacccgcctt ccctcaggcc 73800
caggggctga gctttctgaa gcaaattgaa tcctgctgcc tcagctcaca gcgtctctgg 73860
ggttccttca ctggaagctg ctgcctccct ggttattgac aaaggttact ggcaaaggct 73920
actggcagtt cggtgccaac acgtccctct ggccttgcct catgtccctc ccctgcacca 73980
gccccaagcg ctatctggtc aggctgatac tggaatggcg ctgttgacac tggaatagtc 74040
tttagcacat tccctcggtg aactcctacg tcatcttcaa aaccctttgc aaatgcatcg 74100
ctatggtggg tcttcctcag acaggatgct tgtgccctcc taaacccccc aaaatttctt 74160
atagataaat tgcaggggtc cttgagtttc tcactacacc tggaacagaa ccttgagcca 74220
ttctgttcga aatgctaaag gaaaagataa cccactgagg tggtggtctc aggtccatcc 74280
aagcaactgg cacctgggtc acagatatgt ggcctaattt cgtcccattc ccccgccccc 74340
accttgaccg tgagggaagg gcctgagaag aggcaccagc tgcggctgag aaacagcaga 74400
gaaggaaaag aacaaagctg ggggcgtggg tggcttctgc aagctgagcg gagtggcaag 74460
cttgcaggag tggcaagcgg gctgtgtgcg gagtgggcac acatcccctc cggtggccac 74520
gcctcagtcc tggactttgg ctgtcctggg taggcaggcg ggccaggctg cgccccagca 74580
agcaggaggt gcctggcttc ctcatttggc tgcagccaca gccactgcca ccatgcagag 74640
gaggacctgg gggctgcacc tgctgcttct gctggggctg gggctggggt cctgggaggc 74700
cctgccccca ccctgtgaga g 74721
<210> 2
<211> 22
<212> DNA
<213> Sus scrofa
<400> 2
caccctgtga gagccagatt ta 22
<210> 3
<211> 21
<212> DNA
<213> Sus scrofa
<400> 3
ctgaagcgct gtagtacctg g 21
<210> 4
<211> 22
<212> DNA
<213> Sus scrofa
<400> 4
cctttattct tctctaaccc gt 22
<210> 5
<211> 20
<212> DNA
<213> Sus scrofa
<400> 5
tcagtgagca gagacttgtg 20
<210> 6
<211> 18
<212> DNA
<213> Sus scrofa
<400> 6
gaggagatgg actccacg 18
<210> 7
<211> 18
<212> DNA
<213> Sus scrofa
<400> 7
gggaagccaa ccagttag 18
<210> 8
<211> 19
<212> DNA
<213> Sus scrofa
<400> 8
cagctctaca agaagccga 19
<210> 9
<211> 19
<212> DNA
<213> Sus scrofa
<400> 9
aagagaaagg acgatggca 19
<210> 10
<211> 24
<212> DNA
<213> Sus scrofa
<400> 10
tcctgtactg agctgccccg agtt 24
<210> 11
<211> 23
<212> DNA
<213> Sus scrofa
<400> 11
ctgccctggc ccgagggacc gac 23

Claims (10)

1.一种与猪皮下脂肪相关的circRNA，命名为circRNA_26852，序列与SEQ ID NO.1具有90％以上序列同源性。
2.一种检测皮下脂肪的试剂，所述试剂检测样品circRNA_26852表达水平。
3.权利要求2所述的试剂，其特征在于，采用测序技术、核酸杂交技术或核酸扩增技术检测样品circRNA_26852表达水平。
4.权利要求2所述的试剂，其特征在于，采用高通量测序技术、探针杂交技术、基因芯片技术或荧光定量PCR技术检测样品circRNA_26852的表达水平，优选的，试剂含有与circRNA_26852杂交的探针或扩增circRNA_26852的特异性引物。
5.根据权利要求2所述的试剂，其特征在于，样品为猪皮下脂肪组织。
6.一种检测皮下脂肪的试剂，检测circRNA_26852相关基因的表达水平，其特征在于，相关基因为ssc-miR-486、ssc-miR-874、ssc-miR-486靶基因的ABHD5或ssc-miR-874的靶基因SMAD7和SCD。
7.根据权利要求7所述的试剂，其特征在于，试剂通过测序技术、核酸杂交技术、核酸扩增技术或免疫测定的方法检测circRNA_26852相关基因的表达水平，优选的，试剂含有特异性扩增ssc-miR-486的引物；或特异性扩增ssc-miR-874的引物；或特异性扩增ABHD5的引物；或特异性扩增SMAD7的引物；或特异性扩增SCD的引物。
8.一种检测猪皮下脂肪含量的方法，包括：(1)选取猪皮下脂肪组织；(2)检测样品中circRNA_26852和/或其相关基因的表达量，所述相关基因为ssc-miR-486、ssc-miR-874、ssc-miR-486靶基因的ABHD5或ssc-miR-874的靶基因SMAD7和SCD。
9.下述任意一项应用：
circRNA_26852在预测或辅助预测猪肉品质中的应用；
circRNA_26852在制备预测或辅助预测猪肉品质试剂中的应用；
circRNA_26852在选育具有不同肌肉品质猪中的应用。
权利要求2-5任意一项所述的试剂在预测或辅助预测猪肉品质中的应用；
权利要求2-5任意一项所述的试剂在制备预测或辅助预测猪肉品质试剂中的应用；
权利要求2-5任意一项所述的试剂在选育具有不同肌肉品质猪中的应用。
10.下述任意一项应用：
权利要求6-7任意一项所述试剂在预测或辅助预测猪肉品质中的应用；
权利要求6-7任意一项所述试剂在制备预测或辅助预测猪肉品质试剂中的应用；
权利要求6-7任意一项所述试剂在选育具有不同肌肉品质猪中的应用。
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CN109628446A (zh) * 2018-12-05 2019-04-16 中国农业科学院北京畜牧兽医研究所 circRNA_27455及其检测试剂与应用
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CN109517904A (zh) * 2018-12-05 2019-03-26 中国农业科学院北京畜牧兽医研究所 circRNA_14759及其互作基因的应用
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