CN108841818A - A kind of method for extracting nucleic acid and device - Google Patents
A kind of method for extracting nucleic acid and device Download PDFInfo
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- CN108841818A CN108841818A CN201810203382.6A CN201810203382A CN108841818A CN 108841818 A CN108841818 A CN 108841818A CN 201810203382 A CN201810203382 A CN 201810203382A CN 108841818 A CN108841818 A CN 108841818A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
Abstract
The present invention discloses a kind of method for extracting nucleic acid and device, including sample extraction module, for holding nucleic acid samples and extracting from sample nucleic acid；Magnetic bead syringe pump, for being passed through the solution for being mixed with micron magnetic bead into sample extraction module；Magnetic field generating module, for generating high-intensity magnetic field；Sample dissociation module, for separating nucleic acid from sample；Eluent syringe pump, for being passed through eluent into sample extraction module for nucleic acid and Beads enrichment and recycling magnetic bead and obtain nucleic acid.The present invention provides effective in a kind of biological sample of large volume, quickly, the method and device of semi-automatic extraction nucleic acid, 10 milliliters or more of nucleic acids in samples rapidly extracting may be implemented by this method, extraction time is shorter compared to traditional approach, whole link of extracting can be combined with automatic operation, later period with other detection devices, achieve the purpose that full-automatic detection.
Description
Technical field
The present invention relates to continuous fluid separation fields.More particularly, to a kind of method for extracting nucleic acid and device.
Background technique
Molecular diagnosis is mainly used in different kind organism diagnosis using nucleic acid as test object.Molecule is carried out in sample
Before diagnosis, it is necessary first to extract nucleic acid from sample to be tested, the concentration and purity of nucleic acid directly affect the standard of molecular diagnosis
Exactness.The extraction of traditional nucleic acid generally has the methods of density gradient centrifugation, phenol/chloroform, phenol, isoamyl alcohol extraction, but these
Method is toxic, or time-consuming long, or needs larger complex appts, its method is made to be restricted.In recent years, paramagnetic particle method is increasingly
It is widely used in the extraction of nucleic acids in samples.The general step of this method is as follows：Pass through lysate first for the core of determinand
Acid is cracked from cell and is come out, and then captures nucleic acid by the electrostatic interaction of magnetic bead surfaces, and then applying magnetic field makes to be combined with magnetic
The nucleic acid of pearl is separated with raw sample, then removes magnetic field, and eluent is added, magnetic bead is separated with nucleic acid, passes through Magneto separate again
Obtain nucleic acid after purification.Compared to other methods, this method is more simple, conveniently, and safe and non-toxic, the nucleic acid extracted is dense
Degree and purity are higher.
It is had the disadvantage that however, conventional paramagnetic particle method extracts nucleic acid：Firstly, carrying out the sample volume that paramagnetic particle method extracts nucleic acid
Limited, generally tens microlitres are arrived several hectolambdas.Secondly, the operating procedure that manual paramagnetic particle method extracts nucleic acid is complex, need
Skilled engineer carries out skilled operation；And common automatic nucleic acid extraction element volume is larger on the market, more
Valuableness, and it is not easy portable, it is difficult to meet the needs of on-site test.Finally, the micro-fluidic magnetic bead risen recently extracts nucleic acid method
Sample to be tested volume it is typically small, extraction rate is slower.In view of the above problems, the present invention is extracted from the sample continuously flowed
Nucleic acid provides a kind of analyte capture method of large volume；Provide the example of a lesser semi-automatic device, operation letter
It is single, have portability, meanwhile, acquisition speed is fast, can capture the DNA in a large amount of samples in a short time.
Accordingly, it is desirable to provide a kind of method for extracting nucleic acid and device.
Summary of the invention
The purpose of the present invention is to provide a kind of method for extracting nucleic acid and devices.
In order to achieve the above objectives, the present invention adopts the following technical solutions：
A kind of method for extracting nucleic acid, including：
The solution for being mixed with micron magnetic bead is passed through in container, and the container is placed in magnetic field to the magnetic bead made in solution
It is combined at chain and is full of container；
It is passed through the container after the sample that lysate is added is cracked, makes nucleic acid and the appearance after cracking in sample
Magnetic bead in device combines absorption in the container, and other impurities go out with mixed solution from container outlet stream；
Cleaning solution is passed through the container, the nucleic acid samples after purifying in the container in conjunction with magnetic bead；
Eluent is passed through the container, makes nucleic acid and Beads enrichment in the nucleic acid samples in the container.
Further, it is described the sample that lysate is added is cracked after be passed through the container and include：It is cracked being added
The sample dissociation of liquid is passed through in the container after ten minutes and is kept for 3-5 minutes.
Further, it mixes and is kept for 3-5 minutes with the nucleic acid samples after cleaning solution being passed through the container.
Further, it mixes and is kept for 3-5 minutes with the nucleic acid samples after eluent being passed through the container.
The invention also discloses a kind of nucleic acid-extracting apparatus, including：
Sample extraction module, for holding nucleic acid samples and extracting from sample nucleic acid；
Magnetic bead syringe pump, for being passed through the solution for being mixed with micron magnetic bead into sample extraction module；
Magnetic field generating module, for generating high-intensity magnetic field；
Sample dissociation module, for separating nucleic acid from sample；
Nucleic acid and Beads enrichment are obtained nucleic acid for being passed through eluent into sample extraction module by eluent syringe pump.
Further, the sample extraction module is arc-shaped, including entrance, outlet, shell and inner passage, described interior
Portion channel is S-type to be evenly spaced in shell, and both ends are connect with entrance and exit respectively.
Further, the magnetic field generating module is hollow arc-shaped, including big magnet, small magnet and pedestal, described big
Magnet and small magnet are the setting of the arc-shaped and concyclic heart in pedestal, and the sample extraction module is arranged in big magnet and small magnetic
Between iron, high-intensity magnetic field is generated between the big magnet and small magnet.
Further, the sample dissociation module includes cracking tube channel, heating tube, instlated tubular and temperature probe, described
Cracking tube channel is connect with the sample extraction module inlet, and the heating tube is placed on the cracking channel tube outer surface and heats
The cracking tube channel, the instlated tubular are placed on the heating tube outer surface, distribute for reducing heat, the temperature probe with
The cracking tube channel connection, for measuring the temperature and feedback of the cracking tube channel.
Further, the extraction element further includes cleaning solution syringe pump, the first multi-pass pipe, the second multi-pass pipe and multiple more
Port valve, the cleaning solution syringe pump are used to be passed through the nucleic acid sample after cleaning solution purifies in conjunction with magnetic bead into sample extraction module
Product；One end of the first multi-pass pipe is connected to sample extraction module inlet, the other end respectively with magnetic bead syringe pump, sample dissociation
Module, cleaning solution syringe pump, the connection of eluent syringe pump；Described second multi-pass pipe one end is connected to sample extraction module outlet,
The other end is connected to extract, devil liquor recovery unit respectively.
Further, the extraction element further includes control module, and the control module includes control circuit and micro process
Device, for controlling the working condition of the modules in the extraction element and the open and-shut mode of multiple-way valve.
Beneficial effects of the present invention are as follows：
Technical solution of the present invention provides in a kind of biological sample of large volume effectively, and quickly, semi-automatic mentions
10 milliliters or more of nucleic acids in samples rapidly extracting, extraction time may be implemented by this method for the method and device for taking nucleic acid
Shorter compared to traditional approach, whole link of extracting can be combined with automatic operation, later period with other detection devices, reach complete
The purpose of automatic detection.
Detailed description of the invention
Specific embodiments of the present invention will be described in further detail with reference to the accompanying drawing；
Fig. 1 is a kind of method for extracting nucleic acid flow chart provided by the invention；
Fig. 2 is a kind of nucleic acid-extracting apparatus schematic diagram provided by the invention；
Fig. 3 is sample extraction modular structure schematic diagram provided by the invention；
Fig. 4 is magnetic field generating module structural schematic diagram provided by the invention；
Fig. 5 is sample dissociation modular structure schematic diagram provided by the invention；
Fig. 6 is a kind of nucleic acid-extracting apparatus partial structure diagram provided by the invention.
Wherein, 1, sample extraction module；2, magnetic field generating module；3, control module；4, the first multi-pass pipe；5, the second multi-pass
Pipe；6, sample dissociation module；7, sample injection pumps；8, magnetic bead syringe pump；9, cleaning solution syringe pump；10, eluent syringe pump；
11, nucleic acid collecting pump；12, waste liquid reclaiming pump；13, entrance；14, it exports；15, shell；16, inner passage；17, bolt hole, 18,
Big magnet；19, small magnet；20, pedestal；21, side fixed frame；22, entrance and exit of the passage conduit；23, connecting conduit；24, it cracks
Tube channel；25, instlated tubular；26, heating tube；27, temperature probe.
Specific embodiment
In order to illustrate more clearly of the present invention, the present invention is done further below with reference to preferred embodiments and drawings
It is bright.Similar component is indicated in attached drawing with identical appended drawing reference.It will be appreciated by those skilled in the art that institute is specific below
The content of description is illustrative and be not restrictive, and should not be limited the scope of the invention with this.
As depicted in figs. 1 and 2, a kind of method for extracting nucleic acid disclosed by the invention, including：
S1, the solution for being mixed with micron magnetic bead is passed through in container, and the container is placed in magnetic field makes in solution
Magnetic bead combines at chain and is full of container.
Magnetic bead syringe pump 8 is opened, the solution for being mixed with micron magnetic bead enters sample extraction module 1 by the first multi-pass pipe 4,
It will combine and be full of entire sample extraction module 1 under the action of magnetic field at chain in a reservoir, remaining solution is then from outlet
Outflow.
S2, it is passed through the container after being cracked the sample that lysate is added, makes nucleic acid and institute after cracking in sample
The magnetic bead stated in container combines absorption in the container, and other impurities go out with mixed solution from container outlet stream.
Sample injection pump 7 is opened, the sample that joined lysate is passed through sample dissociation module 6, sample dissociation 10 is divided
Clock, then by the first multi-pass pipe 4 enter sample extraction module 1 in keep 3-5min (or with lesser speed i.e. be not more than
0.5mL/min injects sample extraction module 1), the nucleic acid after cracking at this time is by the chaining magnetic bead surfaces with interior sample extraction module 1
In conjunction with, it is adsorbed in sample extraction module 1, and other impurities in sample etc. will be discharged from outlet.
S3, cleaning solution is passed through the container, the nucleic acid samples after purifying in the container in conjunction with magnetic bead.
Cleaning solution syringe pump 9 is opened, cleaning solution is passed through in sample extraction module 1,3-5min is kept (or to be slowly introducing sample
Product extraction module), to continue to separate the impurity in some samples with nucleic acid, achieve the purpose that purification.
S4, eluent is passed through the container, makes nucleic acid and Beads enrichment in the nucleic acid samples in the container.
Eluent syringe pump 10 is opened, eluent is passed through in sample extraction module 1 and keeps 3min (or with smaller speed
It is not more than the injection channel 0.1mL/min), under the action of eluent, magnetic bead will be separated with the nucleic acid that it is captured, after separation
Nucleic acid flows out together with eluent from outlet.Sample extraction module 1 is taken out from magnetic field generating module 2 after nucleic acid extraction,
And it is passed through the i.e. recyclable magnetic bead of solution.
Invention additionally discloses a kind of nucleic acid-extracting apparatus, as shown in Fig. 2, including：Sample extraction module 1, for holding
Nucleic acid samples simultaneously extract nucleic acid from sample；
Magnetic bead syringe pump 8, for being passed through the solution for being mixed with micron magnetic bead into sample extraction module 1；
Magnetic field generating module 2, for generating high-intensity magnetic field；
Sample dissociation module 6 is used for nucleic acid from separation in sample and in conjunction with magnetic bead；
Nucleic acid and Beads enrichment are obtained core for being passed through eluent into sample extraction module 1 by eluent syringe pump 10
Acid.
Specifically, as shown in figure 3, sample extraction module 1 is arc-shaped, including entrance 13, outlet 14, shell 15 and inside
Channel 16, the inner passage 16 is S-type to be evenly spaced in shell 15, and both ends are connect with entrance 13 and outlet 14 respectively.Shell
Body is arc-shaped shape, material selection biochemistry inert material in the horizontal direction, and anticorrosion material is selected in inner passage 16,
Inside is for liquid communication.
As shown in figure 4, magnetic field generating module 2 is hollow arc-shaped, including big magnet 18, small magnet 19 and pedestal 20, institute
Stating big magnet 18 and small magnet 19 is the setting of the arc-shaped and concyclic heart in pedestal 20, and sample extraction module 1 is arranged in big magnetic
Between iron 18 and small magnet 19, removably it is embedded between big magnet 18 and small magnet 19, two magnet are in the radial direction
The high-strength magnet for being neodymium iron boron N52 for the material from N grades to S grades, in radial directions, especially in sample extraction module 1
Position generates high gradient and high-intensitive magnetic field, in addition, using three bolts in bolt hole 17 fixing side fixed frame the bottom of in
On seat 20.The inner passage 16 of extraction module 1 is consistent with arc magnetic field constructed by magnetic field generating module 2, it is ensured that channel
Each region in 16 keeps same or similar magnetic field conditions, so that magnetic bead uniform chaining in channel 16, therefore it is logical
Whole region is distributed with the magnetic bead that can capture sample in road 16.
As shown in figure 5, sample dissociation module 6 includes cracking tube channel 24, instlated tubular 25, heating tube 26 and temperature probe
27.Cracking tube channel 24 is connect with one of connecting conduit of the first multi-pass pipe 4, and heating tube 26 is used to give cracking tube channel 24
Heating, instlated tubular 25 can reduce cracking 24 conduct heat away of tube channel, temperature probe 27 measure temperature and by temperature feedback to control
Unit 3 processed, control unit 3 pass through the temperature measured and control the work of heating tube 26.
As shown in fig. 6, extraction element further includes cleaning solution syringe pump 9, side fixed frame 21, connecting conduit in conjunction with Fig. 2
24, entrance and exit of the passage conduit 22, cleaning solution syringe pump 9 is for being passed through cleaning solution purification and magnetic bead knot into sample extraction module 1
Nucleic acid samples after conjunction, fixed frame 21 are used for the magnet of fixing side, and the influence to avoid high-intensity magnetic field for surrounding enviroment guarantees
Safety；Conduit 22 one end in entrance and exit of the passage 14 is connected to the entrance 13 of extraction module 1 and outlet respectively, and the other end is respectively with first
Multi-pass pipe 4 is connected to the second multi-pass pipe 5, and liquid is avoided to leak out.First multi-pass pipe 4 and the second multi-pass pipe 5 are equipped with multiple-way valve, control
The on-off of each conduit of multi-pass pipe processed.
It is additionally provided with control module 3, including voltage regulator circuit on extraction element, touch screen display control circuit, microprocessor, two
Multi-pass valve-driving circuit, multiple pump driving circuits and temperature control circuit etc..Single-chip microcontroller or arm processor can be used in microprocessor,
Instruction control driving circuit is assigned, and then controls corresponding valve, pump and heating module；On the one hand touch screen display controller receives micro- place
The control for managing device, provides display function for user, is on the other hand fed back by user's touch screen, transmitting user instruction to micro process
Device；Voltage regulator circuit is microprocessor, and touch screen display controller and driving circuit provide stable voltage, to keep system to stablize.
Technical solution of the present invention provides in a kind of biological sample of large volume effectively, and quickly, semi-automatic mentions
10 milliliters or more of nucleic acids in samples rapidly extracting, extraction time may be implemented by this method for the method and device for taking nucleic acid
Shorter compared to traditional approach, whole link of extracting can be combined with automatic operation, later period with other detection devices, reach complete
The purpose of automatic detection.
Obviously, the above embodiment of the present invention be only to clearly illustrate example of the present invention, and not be pair
The restriction of embodiments of the present invention may be used also on the basis of the above description for those of ordinary skill in the art
To make other variations or changes in different ways, all embodiments can not be exhaustive here, it is all to belong to this hair
The obvious changes or variations that bright technical solution is extended out are still in the scope of protection of the present invention.
Claims (10)
1. a kind of method for extracting nucleic acid, which is characterized in that including：
The solution for being mixed with micron magnetic bead is passed through in container, and the container is placed in magnetic field to the magnetic bead chaining made in solution
Shape combines and is full of container；
It is passed through the container after the sample that lysate is added is cracked, makes in the nucleic acid and the container after cracking in sample
Magnetic bead combine absorption in the container, other impurities go out with mixed solution from container outlet stream；
Cleaning solution is passed through the container, the nucleic acid samples after purifying in the container in conjunction with magnetic bead；
Eluent is passed through the container, makes nucleic acid and Beads enrichment in the nucleic acid samples in the container.
2. method for extracting nucleic acid according to claim 1, which is characterized in that described to split the sample that lysate is added
The container is passed through after solution includes：The sample dissociation that lysate is added is passed through after ten minutes in the container and is kept for 3-5 minutes.
3. method for extracting nucleic acid according to claim 1, which is characterized in that by cleaning solution be passed through after the container with it is described
Nucleic acid samples are mixed and are kept for 3-5 minutes.
4. method for extracting nucleic acid according to claim 1, which is characterized in that by eluent be passed through after the container with it is described
Nucleic acid samples are mixed and are kept for 3-5 minutes.
5. a kind of nucleic acid-extracting apparatus, which is characterized in that including：
Sample extraction module, for holding nucleic acid samples and extracting from sample nucleic acid；
Magnetic bead syringe pump, for being passed through the solution for being mixed with micron magnetic bead into sample extraction module；
Magnetic field generating module, for generating high-intensity magnetic field；
Sample dissociation module is used for nucleic acid from separation in sample and in conjunction with magnetic bead；
Nucleic acid and Beads enrichment are obtained nucleic acid for being passed through eluent into sample extraction module by eluent syringe pump.
6. nucleic acid-extracting apparatus according to claim 5, which is characterized in that the sample extraction module is arc-shaped, packet
Include entrance, outlet, shell and inner passage, the inner passage is S-type to be evenly spaced in shell, both ends respectively with entrance and
Outlet connection.
7. nucleic acid-extracting apparatus according to claim 6, which is characterized in that the magnetic field generating module is hollow circular arc
Shape, including big magnet, small magnet and pedestal, the big magnet and small magnet are the setting of the arc-shaped and concyclic heart in pedestal,
The sample extraction module is arranged between big magnet and small magnet, and high-intensitive magnetic is generated between the big magnet and small magnet
?.
8. nucleic acid-extracting apparatus according to claim 6, which is characterized in that the sample dissociation module includes cracking channel
Pipe, heating tube, instlated tubular and temperature probe, the cracking tube channel are connect with the sample extraction module inlet, the heating
Pipe sleeve is in the cracking channel tube outer surface and heats the cracking tube channel, and the instlated tubular is placed on the heating tube appearance
Face is distributed for reducing heat, and the temperature probe is connect with the cracking tube channel, for measuring the cracking tube channel
Temperature is simultaneously fed back.
9. nucleic acid-extracting apparatus according to claim 6, which is characterized in that the extraction element further includes cleaning solution injection
Pump, the first multi-pass pipe, the second multi-pass pipe and multiple multiple-way valves, the cleaning solution syringe pump into sample extraction module for being passed through
Cleaning solution purification in conjunction with magnetic bead after nucleic acid samples；One end of the first multi-pass pipe is connected to sample extraction module inlet,
The other end is connect with magnetic bead syringe pump, sample dissociation module, cleaning solution syringe pump, eluent syringe pump respectively；More than described second
Siphunculus one end is connected to sample extraction module outlet, and the other end is connected to extract, devil liquor recovery unit respectively.
10. nucleic acid-extracting apparatus according to claim 9, which is characterized in that the extraction element further includes control module,
The control module includes control circuit and microprocessor, for controlling the working condition of the modules in the extraction element
And the open and-shut mode of multiple-way valve.
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109355283A (en) * | 2018-11-27 | 2019-02-19 | 中国科学院上海技术物理研究所 | A kind of nucleic acid automatic extracting device suitable for space |
| CN109679832A (en) * | 2019-01-22 | 2019-04-26 | 周鸿坡 | A kind of high throughput automated magnetic beads for purifying instrument and its working method |
| CN110093403A (en) * | 2019-03-19 | 2019-08-06 | 融智生物科技（青岛）有限公司 | The freeze drying protectant of fluorescent PCR reagent and the preparation method that chip is lyophilized |
| CN110819623A (en) * | 2019-11-08 | 2020-02-21 | 珠海迪尔生物工程有限公司 | Nucleic acid extraction instrument and extraction method |
| CN111440703A (en) * | 2020-05-19 | 2020-07-24 | 广州高盛生物科技股份有限公司 | Double-extraction-mode nucleic acid extraction workstation and nucleic acid extraction method |
| CN112126642A (en) * | 2019-06-25 | 2020-12-25 | 京东方科技集团股份有限公司 | Nucleic acid extraction device and method thereof |
| CN112725162A (en) * | 2021-01-15 | 2021-04-30 | 山东大学 | Full-automatic microbial nucleic acid extraction and detection device and extraction and detection method |
| CN113646419A (en) * | 2019-02-14 | 2021-11-12 | 高丽大学校算学协力团 | Extraction device for extracting target substance, extraction method, and fluidic chip |
| WO2022061521A1 (en) * | 2020-09-22 | 2022-03-31 | 京东方科技集团股份有限公司 | Microfluidic chip for nucleic acid extraction, and nucleic acid extraction device and method |
| WO2022151640A1 (en) * | 2021-01-12 | 2022-07-21 | 厦门致善生物科技股份有限公司 | Device, kit and method for extracting nucleic acids |
| CN117070326A (en) * | 2023-10-19 | 2023-11-17 | 吉林大学 | Nucleic acid extraction device and extraction method thereof |
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| CN109355283A (en) * | 2018-11-27 | 2019-02-19 | 中国科学院上海技术物理研究所 | A kind of nucleic acid automatic extracting device suitable for space |
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| CN109679832A (en) * | 2019-01-22 | 2019-04-26 | 周鸿坡 | A kind of high throughput automated magnetic beads for purifying instrument and its working method |
| CN113646419A (en) * | 2019-02-14 | 2021-11-12 | 高丽大学校算学协力团 | Extraction device for extracting target substance, extraction method, and fluidic chip |
| CN110093403A (en) * | 2019-03-19 | 2019-08-06 | 融智生物科技（青岛）有限公司 | The freeze drying protectant of fluorescent PCR reagent and the preparation method that chip is lyophilized |
| CN112126642A (en) * | 2019-06-25 | 2020-12-25 | 京东方科技集团股份有限公司 | Nucleic acid extraction device and method thereof |
| CN110819623A (en) * | 2019-11-08 | 2020-02-21 | 珠海迪尔生物工程有限公司 | Nucleic acid extraction instrument and extraction method |
| CN111440703A (en) * | 2020-05-19 | 2020-07-24 | 广州高盛生物科技股份有限公司 | Double-extraction-mode nucleic acid extraction workstation and nucleic acid extraction method |
| WO2022061521A1 (en) * | 2020-09-22 | 2022-03-31 | 京东方科技集团股份有限公司 | Microfluidic chip for nucleic acid extraction, and nucleic acid extraction device and method |
| WO2022151640A1 (en) * | 2021-01-12 | 2022-07-21 | 厦门致善生物科技股份有限公司 | Device, kit and method for extracting nucleic acids |
| CN112725162B (en) * | 2021-01-15 | 2022-03-01 | 山东大学 | Full-automatic microbial nucleic acid extraction and detection device and extraction and detection method |
| CN112725162A (en) * | 2021-01-15 | 2021-04-30 | 山东大学 | Full-automatic microbial nucleic acid extraction and detection device and extraction and detection method |
| CN117070326A (en) * | 2023-10-19 | 2023-11-17 | 吉林大学 | Nucleic acid extraction device and extraction method thereof |
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