CN108834909A - A kind of pale reddish brown trident bletilla striata seeds quickly breed the tissue culture method of seedling - Google Patents
A kind of pale reddish brown trident bletilla striata seeds quickly breed the tissue culture method of seedling Download PDFInfo
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- CN108834909A CN108834909A CN201811125636.3A CN201811125636A CN108834909A CN 108834909 A CN108834909 A CN 108834909A CN 201811125636 A CN201811125636 A CN 201811125636A CN 108834909 A CN108834909 A CN 108834909A
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- reddish brown
- bletilla striata
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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Abstract
The present invention relates to seed tissue culture technique fields, and in particular to a kind of pale reddish brown trident bletilla striata seeds quickly breed the tissue culture method of seedling, include the following steps：Step 1, choose stalwartness, full seed, pale reddish brown trident bletilla striata seeds of the same size, first carry out radiation treatment, then washing 4-5 times is carried out with distilled water, 20-30min then is impregnated with 10-20% saline solution, is re-fed into 75% ethanol solution and impregnates 5min, then washed with deionized water, it is drained followed in turn by aseptic paper, it is spare.Pale reddish brown trident bletilla striata seeds of the invention first carry out radiation treatment, cooperate 10% saline solution, 75% ethanol solution again, bactericidal effect can not only be played, seed activity is improved simultaneously, using the sodium selenate solution seed soaking of 300mg/L, the processing of mangrove Litter leaf aqueous extract, selenium can be converted in plant and accumulation, participates in metabolism.
Description
Technical field
The present invention relates to seed tissue culture technique fields, and in particular to a kind of pale reddish brown trident bletilla striata seeds are quickly multiplied into
The tissue culture method of seedling.
Background technique
The pale reddish brown trident bletilla striata has extensive medical value and Ornamental value, is mainly used for astringing to arrest bleeding, detumescence and promoting granulation, Hua You
The colors such as purplish red, white, blue, yellow and powder, can potting indoor appreciation, one jiao of flower stand, garden can be interspersed, currently, the pale reddish brown trident bletilla striata
It is too big for requirement difference, it causes largely to acquire and consumes plant resources, along with the Hills afforestation rate of southern each province
It further increases, wild bletilla striata yield can also be aggravated and declined every year, when the power of regeneration of acquisition and consumption more than natural resources
When, it will lead to the in imminent danger of species, therefore the pale reddish brown trident bletilla striata is quickly bred into seedling and is of great significance.
Existing Chinese patent literature (publication number：CN106417011A it is quickly numerous that a kind of wild bletilla striata tissue cultures) are disclosed
It grows method and is related to a kind of culturing and reproducing of plant, the in particular to culturing and reproducing of drug, it is of the present invention wild
Bletilla striata quick breeding method for tissue culture carries out tissue cultivating using the pseudobulb of the pale reddish brown bletilla striata, after culture medium culture, point
Change degree rate is greatly improved, and can reach 500-900%, to adapt to rapid expansion provenance, transplants after slow seedling, hardening
Survival rate is up to 98%, Chinese patent literature (publication number：CN108308031A a kind of pale reddish brown trident bletilla seed tissue) is disclosed
Cultural method, belongs to bletilla field of tissue culture, a kind of provided pale reddish brown trident bletilla seed tissue cultural method by reserve seed for planting,
Capsule selection, capsule processing, aseptic seeding, seed sprout and from proliferative induction, strong plantlets and rootage, hardening, the bottle outlet sprouted wash seedling,
Sterilisation step carries out tissue cultivating and seedling, and tissue culture method is more conventional in two documents, and plumule emergence is longer, and shoot survival percent is poor,
Quick reproductive effect is poor.
Summary of the invention
The purpose of the present invention is to provide the tissue culture methods that a kind of pale reddish brown trident bletilla striata seeds quickly breed seedling, to solve
The problems mentioned above in the background art.
To achieve the above object, the present invention provides the following technical solutions：
A kind of pale reddish brown trident bletilla striata seeds quickly breed the tissue culture method of seedling, include the following steps：
Step 1 chooses stalwartness, full seed, pale reddish brown trident bletilla striata seeds of the same size, first carries out radiation treatment, with
Washing 4-5 times is carried out with distilled water afterwards, then 20-30min is impregnated with 10-20% saline solution, is re-fed into 75% ethanol solution
5min is impregnated, then is washed with deionized water, is drained followed in turn by aseptic paper, it is spare；
The spare seed of step 1 is used the sodium selenate solution seed soaking of 300-400mg/L by step 2, impregnates 1-2h, with
15-25min is handled with 1-2% mangrove Litter leaf aqueous extract again afterwards, is then rinsed, is drained with clear water, move to culture dish
In, then culture dish is set in the light incubator, cultivation temperature is 26 DEG C, intensity of illumination 400-500umol.m-2.s-1,
Light application time is 6-10h/ days, is cultivated 8-14 days, and protocorm is formed；
Step 2 protocorm is inoculated in proliferated culture medium by step 3, and temperature is maintained at 26 DEG C, auxiliary using ultraviolet irradiation
Illumination cultivation is helped, is then transferred in root media again, then is transferred in differential medium, light filling processing is then carried out, cultivates 15-
25 days, seedling is obtained, then acclimatization and transplants again.
It is as further scheme of the invention：Radiation treatment uses in the step 160Co- gamma-rays carries out at radiation
Reason, amount of radiation 2500-3500Gy, radiation dose rate 1.5-2.5Gy.min-1。
It is as further scheme of the invention：Mangrove Litter leaf aqueous extract preparation method will in the step 2
Mangrove Litter leaf is pulverized, then with solid-liquid ratio 1:100 are added distilled water, are placed on shaking table and are soaked with 200-300r/min
36h is mentioned, is then centrifuged, supernatant is taken.
It is as further scheme of the invention：The proliferated culture medium is MS+6-BA+IBA+ sucrose+agar.
It is as further scheme of the invention：Ultraviolet irradiation fill-in light is with illumination according to condition of culture in the step 3
Intensity is 300-400umol.m-2.s-1, illumination 7h/ days, ultraviolet irradiation 0.5h/ days.
It is as further scheme of the invention：The root media is 1/2MS+IAA+IBA+NAA+ sucrose+agar.
It is as further scheme of the invention：The differential medium is 6-BA differential medium.
It is as further scheme of the invention：The light filling processing is LED sodium yellow lamp source illumination 2h/ days, illumination 5-9
It.
Compared with prior art, the present invention has following beneficial effect：
Pale reddish brown trident bletilla striata seeds of the invention first carry out radiation treatment, then cooperate 10% saline solution, 75% ethanol solution,
Bactericidal effect can be not only played, while improving seed activity, is withered and fallen using sodium selenate solution seed soaking, the mangrove of 300mg/L
The processing of leaf aqueous extract, selenium can be converted in plant and accumulation, participates in metabolism, mangrove Litter leaf aqueous extract contains rich
The growth of pale reddish brown trident bletilla striata survival rate and miaoye can be improved in rich substance, experimental data proof, and step 3 of the present invention is subsequent
Processing, can promote the growth of seedling, improve the time of seminal propagation seedling.
Specific embodiment
Combined with specific embodiments below, technical scheme in the embodiment of the invention is clearly and completely described, shows
So, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based on the reality in the present invention
Example is applied, every other embodiment obtained by those of ordinary skill in the art without making creative efforts all belongs to
In the scope of protection of the invention.
Embodiment 1：
A kind of pale reddish brown trident bletilla striata seeds of the present embodiment quickly breed the tissue culture method of seedling, include the following steps：
Step 1 chooses stalwartness, full seed, pale reddish brown trident bletilla striata seeds of the same size, first carries out radiation treatment, with
Washing 4 times is carried out with distilled water afterwards, then 20min is impregnated with 10% saline solution, is re-fed into 75% ethanol solution and impregnates 5min,
It is washed with deionized water, is drained followed in turn by aseptic paper again, it is spare；
The spare seed of step 1 is used the sodium selenate solution seed soaking of 300mg/L by step 2, impregnates 1h, followed in turn by
1% mangrove Litter leaf aqueous extract handles 15min, is then rinsed, is drained with clear water, moved in culture dish, then will training
Feeding ware is set in the light incubator, and cultivation temperature is 26 DEG C, intensity of illumination 400umol.m-2.s-1, light application time is 6h/ days,
Culture 8 days forms protocorm；
Step 2 protocorm is inoculated in proliferated culture medium by step 3, and temperature is maintained at 26 DEG C, auxiliary using ultraviolet irradiation
Illumination cultivation is helped, is then transferred in root media again, then is transferred in differential medium, light filling processing, culture 15 are then carried out
It, obtains seedling, then acclimatization and transplants again.
Radiation treatment uses in the step of the present embodiment one60Co- gamma-rays progress radiation treatment, amount of radiation 2500Gy,
Radiation dose rate is 1.5Gy.min-1。
Mangrove Litter leaf aqueous extract preparation method grinds mangrove Litter leaf in the step of the present embodiment two
Cheng Fen, then with solid-liquid ratio 1:100 are added distilled water, are placed on shaking table and extract 36h with 200r/min, are then centrifuged, are taken
Supernatant.
The proliferated culture medium of the present embodiment is MS+6-BA+IBA+ sucrose+agar.
It is 300umol.m that ultraviolet irradiation fill-in light, which is with intensity of illumination according to condition of culture, in the step of the present embodiment three-2.s-1, illumination 7h/ days, ultraviolet irradiation 0.5h/ days.
The root media of the present embodiment is 1/2MS+IAA+IBA+NAA+ sucrose+agar.
The differential medium of the present embodiment is 6-BA differential medium.
The light filling processing of the present embodiment is LED sodium yellow lamp source illumination 2h/ days, illumination 5 days.
Embodiment 2：
A kind of pale reddish brown trident bletilla striata seeds of the present embodiment quickly breed the tissue culture method of seedling, include the following steps：
Step 1 chooses stalwartness, full seed, pale reddish brown trident bletilla striata seeds of the same size, first carries out radiation treatment, with
Washing 5 times is carried out with distilled water afterwards, then 30min is impregnated with 20% saline solution, is re-fed into 75% ethanol solution and impregnates 5min,
It is washed with deionized water, is drained followed in turn by aseptic paper again, it is spare；
The spare seed of step 1 is used the sodium selenate solution seed soaking of 400mg/L by step 2, impregnates 1-2h, then again
25min is handled with 2% mangrove Litter leaf aqueous extract, is then rinsed, is drained with clear water, moved in culture dish, then will
Culture dish is set in the light incubator, and cultivation temperature is 26 DEG C, intensity of illumination 500umol.m-2.s-1, light application time 10h/
It, cultivates 14 days, forms protocorm；
Step 2 protocorm is inoculated in proliferated culture medium by step 3, and temperature is maintained at 26 DEG C, auxiliary using ultraviolet irradiation
Illumination cultivation is helped, is then transferred in root media again, then is transferred in differential medium, light filling processing, culture 25 are then carried out
It, obtains seedling, then acclimatization and transplants again.
Radiation treatment uses in the step of the present embodiment one60Co- gamma-rays progress radiation treatment, amount of radiation 3500Gy,
Radiation dose rate is 2.5Gy.min-1。
Mangrove Litter leaf aqueous extract preparation method grinds mangrove Litter leaf in the step of the present embodiment two
Cheng Fen, then with solid-liquid ratio 1:100 are added distilled water, are placed on shaking table and extract 36h with 300r/min, are then centrifuged, are taken
Supernatant.
The proliferated culture medium of the present embodiment is MS+6-BA+IBA+ sucrose+agar.
It is 400umol.m that ultraviolet irradiation fill-in light, which is with intensity of illumination according to condition of culture, in the step of the present embodiment three-2.s-1, illumination 7h/ days, ultraviolet irradiation 0.5h/ days.
The root media of the present embodiment is 1/2MS+IAA+IBA+NAA+ sucrose+agar.
The differential medium of the present embodiment is 6-BA differential medium.
The light filling processing of the present embodiment is LED sodium yellow lamp source illumination 2h/ days, illumination 9 days.
Embodiment 3：
A kind of pale reddish brown trident bletilla striata seeds of the present embodiment quickly breed the tissue culture method of seedling, include the following steps：
Step 1 chooses stalwartness, full seed, pale reddish brown trident bletilla striata seeds of the same size, first carries out radiation treatment, with
Washing 5 times is carried out with distilled water afterwards, then 25min is impregnated with 15% saline solution, is re-fed into 75% ethanol solution and impregnates 5min,
It is washed with deionized water, is drained followed in turn by aseptic paper again, it is spare；
The spare seed of step 1 is used the sodium selenate solution seed soaking of 350mg/L by step 2, impregnates 1.5h, then again
20min is handled with 1.5% mangrove Litter leaf aqueous extract, is then rinsed, is drained with clear water, moved in culture dish, then
Culture dish is set in the light incubator, cultivation temperature is 26 DEG C, intensity of illumination 400-500umol.m-2.s-1, light application time
It is 8h/ days, cultivates 11 days, forms protocorm；
Step 2 protocorm is inoculated in proliferated culture medium by step 3, and temperature is maintained at 26 DEG C, auxiliary using ultraviolet irradiation
Illumination cultivation is helped, is then transferred in root media again, then is transferred in differential medium, light filling processing, culture 20 are then carried out
It, obtains seedling, then acclimatization and transplants again.
Radiation treatment uses in the step of the present embodiment one60Co- gamma-rays carries out radiation treatment, amount of radiation 2500-
3500Gy, radiation dose rate 2.0Gy.min-1。
Mangrove Litter leaf aqueous extract preparation method grinds mangrove Litter leaf in the step of the present embodiment two
Cheng Fen, then with solid-liquid ratio 1:100 are added distilled water, are placed on shaking table and extract 36h with 250r/min, are then centrifuged, are taken
Supernatant.
The proliferated culture medium of the present embodiment is MS+6-BA+IBA+ sucrose+agar.
It is 350umol.m that ultraviolet irradiation fill-in light, which is with intensity of illumination according to condition of culture, in the step of the present embodiment three-2.s-1, illumination 7h/ days, ultraviolet irradiation 0.5h/ days.
The root media of the present embodiment is 1/2MS+IAA+IBA+NAA+ sucrose+agar.
The differential medium of the present embodiment is 6-BA differential medium.
The light filling processing of the present embodiment is LED sodium yellow lamp source illumination 2h/ days, illumination 7 days.
Embodiment 4：
A kind of pale reddish brown trident bletilla striata seeds of the present embodiment quickly breed the tissue culture method of seedling, include the following steps：
Step 1 chooses stalwartness, full seed, pale reddish brown trident bletilla striata seeds of the same size, first carries out radiation treatment, with
Washing 3 times is carried out with distilled water afterwards, then 15min is impregnated with 8% saline solution, is re-fed into 75% ethanol solution and impregnates 5min,
It is washed with deionized water, is drained followed in turn by aseptic paper again, it is spare；
The spare seed of step 1 is used the sodium selenate solution seed soaking of 250mg/L by step 2, impregnates 0.5h, then again
12min is handled with 0.8% mangrove Litter leaf aqueous extract, is then rinsed, is drained with clear water, moved in culture dish, then
Culture dish is set in the light incubator, cultivation temperature is 26 DEG C, intensity of illumination 350umol.m-2.s-1, light application time is
It 5h/ days, cultivates 6 days, forms protocorm；
Step 2 protocorm is inoculated in proliferated culture medium by step 3, and temperature is maintained at 26 DEG C, auxiliary using ultraviolet irradiation
Illumination cultivation is helped, is then transferred in root media again, then is transferred in differential medium, light filling processing, culture 10 are then carried out
It, obtains seedling, then acclimatization and transplants again.
Radiation treatment uses in the step of the present embodiment one60Co- gamma-rays progress radiation treatment, amount of radiation 2000Gy,
Radiation dose rate is 1.0Gy.min-1。
Mangrove Litter leaf aqueous extract preparation method grinds mangrove Litter leaf in the step of the present embodiment two
Cheng Fen, then with solid-liquid ratio 1:100 are added distilled water, are placed on shaking table and extract 36h with 1500r/min, are then centrifuged, are taken
Supernatant.
The proliferated culture medium of the present embodiment is MS+6-BA+IBA+ sucrose+agar.
It is 300- that ultraviolet irradiation fill-in light, which is with intensity of illumination according to condition of culture, in the step of the present embodiment three
400umol.m-2.s-1, illumination 7h/ days, ultraviolet irradiation 0.5h/ days.
The root media of the present embodiment is 1/2MS+IAA+IBA+NAA+ sucrose+agar.
The differential medium of the present embodiment is 6-BA differential medium.
The light filling processing of the present embodiment is LED sodium yellow lamp source illumination 2h/ days, illumination 3 days.
Embodiment 5：
A kind of pale reddish brown trident bletilla striata seeds of the present embodiment quickly breed the tissue culture method of seedling, include the following steps：
Step 1 chooses stalwartness, full seed, pale reddish brown trident bletilla striata seeds of the same size, first carries out radiation treatment, with
Washing 6 times is carried out with distilled water afterwards, then 35min is impregnated with 25% saline solution, is re-fed into 75% ethanol solution and impregnates 5min,
It is washed with deionized water, is drained followed in turn by aseptic paper again, it is spare；
The spare seed of step 1 is used the sodium selenate solution seed soaking of 300-400mg/L by step 2, impregnates 2.5h, with
30min is handled with 3% mangrove Litter leaf aqueous extract again afterwards, is then rinsed, is drained with clear water, moved in culture dish, with
Culture dish is set in the light incubator afterwards, cultivation temperature is 26 DEG C, intensity of illumination 600umol.m-2.s-1, light application time is
It 12h/ days, cultivates 16 days, forms protocorm；
Step 2 protocorm is inoculated in proliferated culture medium by step 3, and temperature is maintained at 26 DEG C, auxiliary using ultraviolet irradiation
Illumination cultivation is helped, is then transferred in root media again, then is transferred in differential medium, light filling processing, culture 30 are then carried out
It, obtains seedling, then acclimatization and transplants again.
Radiation treatment uses in the step of the present embodiment one60Co- gamma-rays carries out radiation treatment, amount of radiation 2500-
3500Gy, radiation dose rate 1.5-2.5Gy.min-1。
Mangrove Litter leaf aqueous extract preparation method grinds mangrove Litter leaf in the step of the present embodiment two
Cheng Fen, then with solid-liquid ratio 1:100 are added distilled water, are placed on shaking table and extract 36h with 350r/min, are then centrifuged, are taken
Supernatant.
The proliferated culture medium of the present embodiment is MS+6-BA+IBA+ sucrose+agar.
It is 450umol.m that ultraviolet irradiation fill-in light, which is with intensity of illumination according to condition of culture, in the step of the present embodiment three-2.s-1, illumination 7h/ days, ultraviolet irradiation 0.5h/ days.
The root media of the present embodiment is 1/2MS+IAA+IBA+NAA+ sucrose+agar.
The differential medium of the present embodiment is 6-BA differential medium.
The light filling processing of the present embodiment is LED sodium yellow lamp source illumination 2h/ days, illumination 11 days.
Comparative example 1.
Using Chinese patent literature (publication number：CN106417011A it is quickly numerous that a kind of wild bletilla striata tissue cultures) are disclosed
It grows method and is related to the pale reddish brown trident bletilla striata seedling of method tissue culture of embodiment 1 in a kind of culturing and reproducing of plant.
Comparative example 2.
The pale reddish brown trident bletilla striata seedling that common tissue culture method obtains.
Embodiment 1-5 and comparative example 1-2 performance measurements are as follows
| Shoot survival percent (%) | Miaoye is wide (mm) | Seedling leaf base diameter (mm) | |
| Embodiment 1 | 98.3 | 11 | 4.0 |
| Embodiment 2 | 98.5 | 11 | 3.9 |
| Embodiment 3 | 98.9 | 12 | 4.2 |
| Embodiment 4 | 97.7 | 10 | 3.8 |
| Embodiment 5 | 97.2 | 10 | 3.7 |
| Comparative example 1 | 93.5 | 9 | 3.3 |
| Comparative example 2 | 91.4 | 7 | 3.1 |
It is obvious to a person skilled in the art that invention is not limited to the details of the above exemplary embodiments, Er Qie
In the case where without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended power
Benefit requires rather than above description limits, it is intended that all by what is fallen within the meaning and scope of the equivalent elements of the claims
Variation is included within the present invention.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiments being understood that.
Claims (8)
1. the tissue culture method that a kind of pale reddish brown trident bletilla striata seeds quickly breed seedling, which is characterized in that include the following steps：
Step 1 chooses stalwartness, full seed, pale reddish brown trident bletilla striata seeds of the same size, first carries out radiation treatment, then uses
Distilled water carries out washing 4-5 times, then impregnates 20-30min with 10-20% saline solution, is re-fed into 75% ethanol solution and impregnates
5min, then washed with deionized water, it is drained followed in turn by aseptic paper, it is spare；
The spare seed of step 1 is used the sodium selenate solution seed soaking of 300-400mg/L by step 2, impregnates 1-2h, then again
15-25min is handled with 1-2% mangrove Litter leaf aqueous extract, is then rinsed, is drained with clear water, moved in culture dish, with
Culture dish is set in the light incubator afterwards, cultivation temperature is 26 DEG C, intensity of illumination 400-500umol.m-2.s-1, when illumination
Between be 6-10h/ days, cultivate 8-14 days, formation protocorm；
Step 2 protocorm is inoculated in proliferated culture medium by step 3, and temperature is maintained at 26 DEG C, using ultraviolet irradiation fill-in light
It according to culture, is then transferred in root media, then is transferred in differential medium again, then carry out light filling processing, cultivate 15-25
It, obtains seedling, then acclimatization and transplants again.
2. a kind of pale reddish brown trident bletilla striata seeds according to claim 1 quickly breed the tissue culture method of seedling, feature exists
In radiation treatment uses in the step 160Co- gamma-rays carries out radiation treatment, amount of radiation 2500-3500Gy, radiation agent
Dose rate is 1.5-2.5Gy.min-1。
3. a kind of pale reddish brown trident bletilla striata seeds according to claim 1 quickly breed the tissue culture method of seedling, feature exists
In, mangrove Litter leaf aqueous extract preparation method pulverizes mangrove Litter leaf in the step 2, then with
Solid-liquid ratio 1:100 are added distilled water, are placed on shaking table and extract 36h with 200-300r/min, are then centrifuged, take supernatant,
?.
4. a kind of pale reddish brown trident bletilla striata seeds according to claim 1 quickly breed the tissue culture method of seedling, feature exists
In the proliferated culture medium is MS+6-BA+IBA+ sucrose+agar.
5. a kind of pale reddish brown trident bletilla striata seeds according to claim 1 quickly breed the tissue culture method of seedling, feature exists
In it is 300-400umol.m that ultraviolet irradiation fill-in light, which is with intensity of illumination according to condition of culture, in the step 3-2.s-1, illumination
7h/ days, ultraviolet irradiation 0.5h/ days.
6. a kind of pale reddish brown trident bletilla striata seeds according to claim 1 quickly breed the tissue culture method of seedling, feature exists
In the root media is 1/2MS+IAA+IBA+NAA+ sucrose+agar.
7. a kind of pale reddish brown trident bletilla striata seeds according to claim 1 quickly breed the tissue culture method of seedling, feature exists
In the differential medium is 6-BA differential medium.
8. a kind of pale reddish brown trident bletilla striata seeds according to claim 1 quickly breed the tissue culture method of seedling, feature exists
In the light filling processing is LED sodium yellow lamp source illumination 2h/ days, illumination 5-9 days.
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| CN201811125636.3A CN108834909A (en) | 2018-09-26 | 2018-09-26 | A kind of pale reddish brown trident bletilla striata seeds quickly breed the tissue culture method of seedling |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109452171A (en) * | 2018-11-26 | 2019-03-12 | 丽江海贝瑞生物科技有限公司 | A kind of tissue culture method of the sterile induction plant regeneration of pale reddish brown trident bletilla striata seeds |
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2018
- 2018-09-26 CN CN201811125636.3A patent/CN108834909A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109452171A (en) * | 2018-11-26 | 2019-03-12 | 丽江海贝瑞生物科技有限公司 | A kind of tissue culture method of the sterile induction plant regeneration of pale reddish brown trident bletilla striata seeds |
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