CN108823264A - A method of using rice as waste high-purity maltose syrup - Google Patents
A method of using rice as waste high-purity maltose syrup Download PDFInfo
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- CN108823264A CN108823264A CN201810807208.2A CN201810807208A CN108823264A CN 108823264 A CN108823264 A CN 108823264A CN 201810807208 A CN201810807208 A CN 201810807208A CN 108823264 A CN108823264 A CN 108823264A
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B30/00—Preparation of starch, degraded or non-chemically modified starch, amylose, or amylopectin
- C08B30/12—Degraded, destructured or non-chemically modified starch, e.g. mechanically, enzymatically or by irradiation; Bleaching of starch
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B30/00—Preparation of starch, degraded or non-chemically modified starch, amylose, or amylopectin
- C08B30/12—Degraded, destructured or non-chemically modified starch, e.g. mechanically, enzymatically or by irradiation; Bleaching of starch
- C08B30/18—Dextrin, e.g. yellow canari, white dextrin, amylodextrin or maltodextrin; Methods of depolymerisation, e.g. by irradiation or mechanically
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/12—Disaccharides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/16—Preparation of compounds containing saccharide radicals produced by the action of an alpha-1, 6-glucosidase, e.g. amylose, debranched amylopectin
Abstract
The invention discloses a kind of using rice as the method for waste high-purity maltose syrup, includes the following steps：S1, rice is eluriated and is impregnated, rice is beaten and obtains rice slurries, liquid will discarded supernatant after the centrifugation of rice slurries, obtain rice precipitating：S2, by rice precipitate in the phosphate buffer of alkali protease containing Alcalace and cellulase is added, discard supernatant liquid after standing after rice precipitating is broken up, precipitating using whizzer be dried to obtain rice starch powder；S3, rice starch powder is made to disperse, and adds Thermostable α-Amylase gelatinization and is liquefied as farinaceous size；S4, debranching enzym solution enzyme and alpha-glucosidase inhibitor are added into farinaceous size, react farinaceous size to obtain malt syrup after mixing evenly.The present invention can inhibit conversion of the maltose to grape slurry in the production process of malt syrup by the way that alpha-glucosidase inhibitor is added during preparing malt syrup, to improve the purity of malt syrup.
Description
Technical field
The present invention relates to food processing technology fields, more particularly to one kind is using rice as waste high-purity maltose syrup
Method.
Background technique
The main application that starch sugar is always old rice or equal low values rice of cracking rice is produced, and malt syrup is then a kind of important
Starch sugar product, according to the height of maltose content, malt syrup can be divided into common malt syrup（Maltose≤60%）, it is high
Malt syrup（Maltose 60~80%）And superhigh maltose syrup（Maltose >=80%）, high maltose syrup is a kind of maltose content
Height, the low medium conversion syrup of glucose content, its advantages are that clear and transparent, infusion temperature is high, sugariness is moderate, moisture retention
Well, resistive connection is brilliant, moisture protection is strong, prevents saprodontia etc., and has certain Functions of Physiological Health Care, therefore have and extremely widely answer
Use prospect.The maltose of existing domestic production generally only has 45% or so containing germinatus sugar amount, and being generally called this maltose is maltose, wheat
Bud sugar generally uses purified starch in process of production to the more demanding of raw material, due to cost of material height, the cost of product
Greatly.Therefore, it is the method for waste high-purity maltose syrup for solving the above problems that we have proposed a kind of using rice.
Summary of the invention
The purpose of the present invention is to solve exist in the prior art maltose in process of production to the requirement of raw material compared with
High disadvantage, and propose a kind of using rice as the method for waste high-purity maltose syrup.
A method of using rice as waste high-purity maltose syrup, include the following steps：
S1, by rice using distilled water eluriate 3-4 time, and by the rice in steep of 1kg in distilled water for 24 hours, by rice give pulled out
It is put into rice beater after coming, and the distilled water of 900ml is added, rice is beaten and obtains rice slurries, by rice slurries
Liquid is discarded supernatant after centrifugation, obtains rice precipitating：
S2, by rice precipitate in be added alkali protease containing Alcalace and cellulase phosphate buffer, rice is precipitated
10min is stood after breaing up, and discards supernatant liquid after being centrifuged 10min under conditions of 6000rpm/min, and precipitating is used into distilled water
After cleaning 5 times, precipitating is dried to obtain rice starch powder using whizzer；
S3, rice starch powder is uniformly dispersed under the action of ultrasonic wave using distilled water, distilled water is heated to 80 DEG C, and
Thermostable α-Amylase is added, and distilled water is constantly stirred, rice starch powder is gelatinized and is liquefied as farinaceous size；
S4, debranching enzym solution enzyme and alpha-glucosidase inhibitor are added into farinaceous size, will form sediment after mixing evenly
Slurry liquid insulation reaction under conditions of 65 DEG C inactivates 10min under conditions of 95 DEG C after for 24 hours and obtains malt syrup.
Preferably, the height of distilled water is 10cm higher than the height of rice during the rice in steep.
Preferably, the pH of the phosphate buffer is 8.0, and the concentration of phosphate buffer is 0.2mol/L, Alcalace alkali
Property protease concentration be 1U/L-1.5U/L, the concentration of cellulase is 0.8U/L-1.2U/L.
Preferably, the weight ratio of the rice starch powder and distilled water is 1:5.
Preferably, it is 6.5 that the farinaceous size, which is measured using pH meter and pH is used to adjust liquid to adjust the pH of farinaceous size,.
Preferably, the concentration of the debranching enzym solution enzyme is 0.30U/ml-0.35U/ml, alpha-glucosidase suppression
The concentration of preparation is 0.15U/ml-0.18U/ml.
The beneficial effects of the invention are as follows：
1, of the invention, by directlying adopt rice, to be raw material make malt syrup, and raw material sources are extensive, and the source of raw material
Securely and reliably, it is suitble to promote and use on a large scale.
2, of the invention, rice starch is obtained by using Alcalace alkali protease and cellulase, so that obtaining
Acquisition condition of the rice starch in acquisition process it is milder, can be energy saving, and the rice starch being prepared
Quality it is high.
3, of the invention, by the way that alpha-glucosidase inhibitor is added during preparing malt syrup, can inhibit making
Conversion of the maltose to grape slurry during malt syrup, to improve the purity of malt syrup.
Specific embodiment
Combined with specific embodiments below the present invention is made further to explain.
Embodiment one：A method of using rice as waste high-purity maltose syrup, include the following steps：
S1, by rice using distilled water eluriate 3 times, and by the rice in steep of 1kg in distilled water for 24 hours（The process of rice in steep
The height of middle distilled water is 10cm higher than the height of rice）, rice is given after pulling out and is put into rice beater, and be added
Rice is beaten and obtains rice slurries by the distilled water of 900ml, will discard supernatant liquid after the centrifugation of rice slurries, obtains rice precipitating：
S2, by rice precipitate in be added alkali protease containing Alcalace and cellulase phosphate buffer（Phosphate buffer
PH be 8.0, the concentration of phosphate buffer is 0.2mol/L, and the concentration of Alcalace alkali protease is 1U/L, cellulase
Concentration be 0.8U/L）, 10min is stood after rice precipitating is broken up, and under conditions of 6000rpm/min after centrifugation 10min
Liquid is discarded supernatant, precipitating is made wash with distilled water after 5 times, precipitating is dried to obtain rice starch powder using whizzer
End；
S3, rice starch powder is uniformly dispersed under the action of ultrasonic wave using the distilled water of 5 times of weight, distilled water is heated
To 80 DEG C, and Thermostable α-Amylase is added, and distilled water is constantly stirred, rice starch powder is gelatinized and is liquefied as to form sediment
Slurry liquid（It is 6.5 that farinaceous size, which is measured using pH meter and pH is used to adjust liquid to adjust the pH of farinaceous size,）；
S4, debranching enzym solution enzyme and concentration that into farinaceous size, addition concentration is 0.30U/ml are 0.15U/ml
Alpha-glucosidase inhibitor, after mixing evenly by farinaceous size under conditions of 65 DEG C insulation reaction for 24 hours after in 95 DEG C of condition
Lower inactivation 10min obtains malt syrup.
Embodiment two：A method of using rice as waste high-purity maltose syrup, include the following steps：
S1, by rice using distilled water eluriate 4 times, and by the rice in steep of 1kg in distilled water for 24 hours（The process of rice in steep
The height of middle distilled water is 10cm higher than the height of rice）, rice is given after pulling out and is put into rice beater, and be added
Rice is beaten and obtains rice slurries by the distilled water of 900ml, will discard supernatant liquid after the centrifugation of rice slurries, obtains rice precipitating：
S2, by rice precipitate in be added alkali protease containing Alcalace and cellulase phosphate buffer（Phosphate buffer
PH be 8.0, the concentration of phosphate buffer is 0.2mol/L, and the concentration of Alcalace alkali protease is 1.2U/L, cellulose
The concentration of enzyme is 1.0U/L）, 10min is stood after rice precipitating is broken up, and be centrifuged 10min under conditions of 6000rpm/min
After discard supernatant liquid, precipitating is made wash with distilled water after 5 times, precipitating is dried to obtain rice starch powder using whizzer
End；
S3, rice starch powder is uniformly dispersed under the action of ultrasonic wave using the distilled water of 5 times of weight, distilled water is heated
To 80 DEG C, and Thermostable α-Amylase is added, and distilled water is constantly stirred, rice starch powder is gelatinized and is liquefied as to form sediment
Slurry liquid（It is 6.5 that farinaceous size, which is measured using pH meter and pH is used to adjust liquid to adjust the pH of farinaceous size,）；
S4, debranching enzym solution enzyme and concentration that into farinaceous size, addition concentration is 0.32U/ml are 0.16U/ml
Alpha-glucosidase inhibitor, after mixing evenly by farinaceous size under conditions of 65 DEG C insulation reaction for 24 hours after in 95 DEG C of condition
Lower inactivation 10min obtains malt syrup.
Embodiment three：A method of using rice as waste high-purity maltose syrup, include the following steps：
S1, by rice using distilled water eluriate 3 times, and by the rice in steep of 1kg in distilled water for 24 hours（The process of rice in steep
The height of middle distilled water is 10cm higher than the height of rice）, rice is given after pulling out and is put into rice beater, and be added
Rice is beaten and obtains rice slurries by the distilled water of 900ml, will discard supernatant liquid after the centrifugation of rice slurries, obtains rice precipitating：
S2, by rice precipitate in be added alkali protease containing Alcalace and cellulase phosphate buffer（Phosphate buffer
PH be 8.0, the concentration of phosphate buffer is 0.2mol/L, and the concentration of Alcalace alkali protease is 1.3U/L, cellulose
The concentration of enzyme is 1.2U/L）, 10min is stood after rice precipitating is broken up, and be centrifuged 10min under conditions of 6000rpm/min
After discard supernatant liquid, precipitating is made wash with distilled water after 5 times, precipitating is dried to obtain rice starch powder using whizzer
End；
S3, rice starch powder is uniformly dispersed under the action of ultrasonic wave using the distilled water of 5 times of weight, distilled water is heated
To 80 DEG C, and Thermostable α-Amylase is added, and distilled water is constantly stirred, rice starch powder is gelatinized and is liquefied as to form sediment
Slurry liquid（It is 6.5 that farinaceous size, which is measured using pH meter and pH is used to adjust liquid to adjust the pH of farinaceous size,）；
S4, debranching enzym solution enzyme and concentration that into farinaceous size, addition concentration is 0.34U/ml are 0.15U/ml
Alpha-glucosidase inhibitor, after mixing evenly by farinaceous size under conditions of 65 DEG C insulation reaction for 24 hours after in 95 DEG C of condition
Lower inactivation 10min obtains malt syrup.
Example IV：A method of using rice as waste high-purity maltose syrup, include the following steps：
S1, by rice using distilled water eluriate 4 times, and by the rice in steep of 1kg in distilled water for 24 hours（The process of rice in steep
The height of middle distilled water is 10cm higher than the height of rice）, rice is given after pulling out and is put into rice beater, and be added
Rice is beaten and obtains rice slurries by the distilled water of 900ml, will discard supernatant liquid after the centrifugation of rice slurries, obtains rice precipitating：
S2, by rice precipitate in be added alkali protease containing Alcalace and cellulase phosphate buffer（Phosphate buffer
PH be 8.0, the concentration of phosphate buffer is 0.2mol/L, and the concentration of Alcalace alkali protease is 1.5U/L, cellulose
The concentration of enzyme is 1.2U/L）, 10min is stood after rice precipitating is broken up, and be centrifuged 10min under conditions of 6000rpm/min
After discard supernatant liquid, precipitating is made wash with distilled water after 5 times, precipitating is dried to obtain rice starch powder using whizzer
End；
S3, rice starch powder is uniformly dispersed under the action of ultrasonic wave using the distilled water of 5 times of weight, distilled water is heated
To 80 DEG C, and Thermostable α-Amylase is added, and distilled water is constantly stirred, rice starch powder is gelatinized and is liquefied as to form sediment
Slurry liquid（It is 6.5 that farinaceous size, which is measured using pH meter and pH is used to adjust liquid to adjust the pH of farinaceous size,）；
S4, debranching enzym solution enzyme and concentration that into farinaceous size, addition concentration is 0.35U/ml are 0.18U/ml
Alpha-glucosidase inhibitor, after mixing evenly by farinaceous size under conditions of 65 DEG C insulation reaction for 24 hours after in 95 DEG C of condition
Lower inactivation 10min obtains malt syrup.
Comparative example：A method of using rice as waste high-purity maltose syrup, include the following steps：
S1, by rice using distilled water eluriate 3 times, and by the rice in steep of 1kg in distilled water for 24 hours（The process of rice in steep
The height of middle distilled water is 10cm higher than the height of rice）, rice is given after pulling out and is put into rice beater, and be added
Rice is beaten and obtains rice slurries by the distilled water of 900ml, will discard supernatant liquid after the centrifugation of rice slurries, obtains rice precipitating：
S2, by rice precipitate in be added alkali protease containing Alcalace and cellulase phosphate buffer（Phosphate buffer
PH be 8.0, the concentration of phosphate buffer is 0.2mol/L, and the concentration of Alcalace alkali protease is 1.3U/L, cellulose
The concentration of enzyme is 1.2U/L）, 10min is stood after rice precipitating is broken up, and be centrifuged 10min under conditions of 6000rpm/min
After discard supernatant liquid, precipitating is made wash with distilled water after 5 times, precipitating is dried to obtain rice starch powder using whizzer
End；
S3, rice starch powder is uniformly dispersed under the action of ultrasonic wave using the distilled water of 5 times of weight, distilled water is heated
To 80 DEG C, and Thermostable α-Amylase is added, and distilled water is constantly stirred, rice starch powder is gelatinized and is liquefied as to form sediment
Slurry liquid（It is 6.5 that farinaceous size, which is measured using pH meter and pH is used to adjust liquid to adjust the pH of farinaceous size,）；
S4, the debranching enzym solution enzyme that concentration is 0.34U/ml is added into farinaceous size, will forms sediment after mixing evenly
Slurry liquid insulation reaction under conditions of 65 DEG C inactivates 10min under conditions of 95 DEG C after for 24 hours and obtains malt syrup.
By the maltose in malt syrup made of embodiment one, embodiment two, embodiment three, example IV and comparative example
Content is measured, and measurement result is as follows：
| Embodiment one | Embodiment two | Embodiment three | Example IV | Comparative example | |
| Maltose content（%） | 79.8 | 81.2 | 82.3 | 80.6 | 56.8 |
Above-mentioned measurement result it is found that in malt syrup made of embodiment one, embodiment two, embodiment three and example IV
Maltose content it is high, and the maltose content highest in manufactured malt syrup made of embodiment three, therefore embodiment
Third is that highly preferred embodiment of the present invention.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (6)
1. a kind of using rice as the method for waste high-purity maltose syrup, which is characterized in that include the following steps：
S1, by rice using distilled water eluriate 3-4 time, and by the rice in steep of 1kg in distilled water for 24 hours, by rice give pulled out
It is put into rice beater after coming, and the distilled water of 900ml is added, rice is beaten and obtains rice slurries, by rice slurries
Liquid is discarded supernatant after centrifugation, obtains rice precipitating：
S2, by rice precipitate in be added alkali protease containing Alcalace and cellulase phosphate buffer, rice is precipitated
10min is stood after breaing up, and discards supernatant liquid after being centrifuged 10min under conditions of 6000rpm/min, and precipitating is used into distilled water
After cleaning 5 times, precipitating is dried to obtain rice starch powder using whizzer；
S3, rice starch powder is uniformly dispersed under the action of ultrasonic wave using distilled water, distilled water is heated to 80 DEG C, and
Thermostable α-Amylase is added, and distilled water is constantly stirred, rice starch powder is gelatinized and is liquefied as farinaceous size；
S4, debranching enzym solution enzyme and alpha-glucosidase inhibitor are added into farinaceous size, will form sediment after mixing evenly
Slurry liquid insulation reaction under conditions of 65 DEG C inactivates 10min under conditions of 95 DEG C after for 24 hours and obtains malt syrup.
2. according to claim 1 a kind of using rice as the method for waste high-purity maltose syrup, which is characterized in that
The height of distilled water is 10cm higher than the height of rice during the rice in steep.
3. according to claim 1 a kind of using rice as the method for waste high-purity maltose syrup, which is characterized in that
The pH of the phosphate buffer is 8.0, and the concentration of phosphate buffer is 0.2mol/L, and the concentration of Alcalace alkali protease is
1U/L-1.5U/L the concentration of cellulase is 0.8U/L-1.2U/L.
4. according to claim 1 a kind of using rice as the method for waste high-purity maltose syrup, which is characterized in that
The weight ratio of the rice starch powder and distilled water is 1:5.
5. according to claim 1 a kind of using rice as the method for waste high-purity maltose syrup, which is characterized in that
It is 6.5 that the farinaceous size, which is measured using pH meter and pH is used to adjust liquid to adjust the pH of farinaceous size,.
6. according to claim 1 a kind of using rice as the method for waste high-purity maltose syrup, which is characterized in that
The concentration of the debranching enzym solution enzyme is 0.30U/ml-0.35U/ml, and the concentration of alpha-glucosidase inhibitor is
0.15U/ml-0.18U/ml。
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109943607A (en) * | 2019-04-08 | 2019-06-28 | 广州市合利源食品有限公司 | Maltose and preparation method thereof |
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2018
- 2018-07-21 CN CN201810807208.2A patent/CN108823264A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109943607A (en) * | 2019-04-08 | 2019-06-28 | 广州市合利源食品有限公司 | Maltose and preparation method thereof |
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