CN108822318A - 一种聚乙二醇改性胶原膜及其应用 - Google Patents

一种聚乙二醇改性胶原膜及其应用 Download PDF

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CN108822318A
CN108822318A CN201810697806.9A CN201810697806A CN108822318A CN 108822318 A CN108822318 A CN 108822318A CN 201810697806 A CN201810697806 A CN 201810697806A CN 108822318 A CN108822318 A CN 108822318A
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collagem membrane
poly ethyldiol
ethyldiol modified
collagen
collagem
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满毅
朱宸佑
向琳
邓佳
伍颖颖
刘丽
屈依丽
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Sichuan University
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    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J5/00—Manufacture of articles or shaped materials containing macromolecular substances
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
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    • A—HUMAN NECESSITIES
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    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
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    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
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    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
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    • C08J2471/00—Characterised by the use of polyethers obtained by reactions forming an ether link in the main chain; Derivatives of such polymers
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Abstract

本发明提供了一种聚乙二醇改性胶原膜，制备方法如下：以培养板为模具，取胶原蛋白2ml，加入浓度为10％w/v醋酸溶液中制备胶原膜；按照1000mg：1ml的比例将制备的300mg胶原膜置于1％w/v聚乙二醇溶液中交联，清洗，干燥，即得。本发明的优点在于：本发明胶原膜拥有更好的能力诱导颅顶骨缺损的修复，提高了以前胶原膜的生物性能，具有很好的应用前景，为引导再生术等相关增量手术提供了新选择。

Description

一种聚乙二醇改性胶原膜及其应用
技术领域
本发明属于生物材料领域，具体涉及一种聚乙二醇改性胶原膜及其应用。
背景技术
牙科手术中常需要应用引导骨再生手术进行骨增量。胶原是细胞外基质的一种成分，已经广泛应用于引导骨再生术中。胶原膜有较好的生物兼容性，并能促进成骨细胞黏附、增殖、迁移和分化，也能有阻挡成纤维细胞快速入侵骨组织生长位点、隔开软组织与硬组织的生长等屏障功能。
然而，作为一种外源性移植物，胶原仍然会引起一定的炎症反应，同时也因为它的易降解性而缺乏较好的机械性能。本领域常用采用戊二醛与胶原进行交联，从而提高它的机械性能，但容易引发炎症和产生细胞毒性。因此，胶原膜长期的保存和使用，不仅仅需要提高机械性能，更要抗炎和提高生物兼容性。
发明内容
本发明的目的是针对现有技术中存在的不足，提供一种聚乙二醇改性胶原膜及其应用。
为了达到上述目的，本发明采用了下列技术方案：一种聚乙二醇改性胶原膜，制备方法如下：
A、以培养板为模具，取胶原蛋白2ml，加入浓度为10％w/v醋酸溶液中制备胶原膜；
B、按照1000mg：1ml的比例将制备的300mg胶原膜置于1％w/v聚乙二醇溶液中交联，清洗，干燥，即得。
作为优选，所述胶原蛋白为牛来源I型胶原。
作为优选，所述步骤A中，在-26℃温度下冷冻过夜，在-53℃、1Pa条件下，真空冷冻干燥24h。
作为优选，所述步骤B中，交联时间为12h。
作为优选，所述步骤B中，将制备好的胶原膜置于-20℃条件下冷冻干燥过夜。
作为优选，所述步骤B中，清洗采用1XPBS缓冲液漂洗3次。
所述改性胶原在医疗领域的应用。
本发明的优点在于：聚乙二醇是一种低毒性的材料，不会引起严重的排异反应。许多研究已经利用聚乙二醇改性材料表面并植入人体，得到了良好的结果。美国食品与药品监督管理局也批准了相应的材料可以在临床上使用。
本发明将胶原膜以聚乙二醇改性，进而得到促骨再生性能良好的聚乙二醇胶原复合膜，有望于应用在引导骨组织再生手术(GBR)中。
本发明胶原膜拥有更好的能力诱导颅顶骨缺损的修复，提高了以前胶原膜的生物性能，具有很好的应用前景，为引导再生术等相关增量手术提供了新选择。
附图说明
图1是实施例3扫描电子显微镜(SEM)检测表面形态实验结果图。
图2是实施例3HE染色实验结果图。
图3是实施例3Masson染色实验结果图。
具体实施方式
下面结合附图，对本发明作详细的说明。
为了使本发明的目的、技术方案及优点更加清楚明白，以下结合附图及实施例，对本发明进行进一步详细说明。应当理解，此处所描述的具体实施例仅仅用以解释本发明，并不用于限定本发明。
实施例1：一种聚乙二醇改性胶原膜，包括如下制备步骤：
1.胶原膜的制备
以直径为3.5cm/孔的培养板为模具，取2mL胶原蛋白加入浓度为10％(w/v)醋酸溶液。于-26℃低温冰箱冷冻过夜，在-53℃、1Pa条件下，冻干机真空冷冻干燥24h，制备胶原膜。
2.配制聚乙二醇(1％w/v)溶液。
3.聚乙二醇改性的胶原膜
将制备的胶原膜置于1％w/v聚乙二醇溶液中改性12h后，lXPBS缓冲液漂洗3次。
将制备好的聚乙二醇改性胶原膜于-20℃条件下冷冻干燥过夜
实施例2：一种聚乙二醇改性胶原膜，，制备方法如下：
A、以培养板为模具，取胶原蛋白2ml，加入浓度为10％w/v醋酸溶液中制备胶原膜；
B、按照1000mg：1ml的比例将制备的300mg胶原膜置于1％w/v聚乙二醇溶液中交联，清洗，干燥，即得。
所述胶原蛋白为牛来源I型胶原。
所述步骤A中，在-26℃温度下冷冻过夜，在-53℃、1Pa条件下，真空冷冻干燥24h。
所述步骤B中，交联时间为12h。
所述步骤B中，将制备好的胶原膜置于-20℃条件下冷冻干燥过夜。
所述步骤B中，清洗采用1XPBS缓冲液漂洗3次。
实施例3：分别取等量的胶原膜、聚乙二醇改性胶原膜，作为实验对比组。
1.扫描电子显微镜(SEM)检测表面形态
扫描式电子显微镜观察材料表面形态，分别为胶原膜、聚乙二醇改性胶原膜。如图1所示，聚乙二醇改性胶原膜表面变的更为平滑。
2.HE染色观察骨再生情况
如图2所示，聚乙二醇改性的胶原膜组别植入大鼠颅顶骨缺损(圆形，直径：5mm)，明显促进骨再生。
3.Masson染色胶原沉积
如图3所示，聚乙二醇改性的胶原膜组别明显促进胶原的沉积。
本发明并不局限于前述的具体实施方式。本发明扩展到任何在本说明书中披露的新特征或任何新的组合，以及披露的任一新的方法或过程的步骤或任何新的组合。

Claims (7)

1.一种聚乙二醇改性胶原膜，其特征在于制备方法如下：
A、以培养板为模具，取胶原蛋白2ml，加入浓度为10％w/v醋酸溶液中制备胶原膜；
B、按照1000mg：1ml的比例将制备的300mg胶原膜置于1％w/v聚乙二醇溶液中交联，清洗，干燥，即得。
2.根据权利要求1所述的一种聚乙二醇改性胶原膜，其特征在于：所述胶原蛋白为牛来源I型胶原。
3.根据权利要求1所述的一种聚乙二醇改性胶原膜，其特征在于：所述步骤A中，在-26℃温度下冷冻过夜，在-53℃、1Pa条件下，真空冷冻干燥24h。
4.根据权利要求1所述的一种聚乙二醇改性胶原膜，其特征在于：所述步骤B中，交联时间为12h。
5.根据权利要求1所述的一种聚乙二醇改性胶原膜，其特征在于：所述步骤B中，将制备好的胶原膜置于-20℃条件下冷冻干燥过夜。
6.根据权利要求1所述的一种聚乙二醇改性胶原膜，其特征在于：所述步骤B中，清洗采用1XPBS缓冲液漂洗3次。
7.根据权利要求1-6任一所述的一种聚乙二醇改性胶原膜，其特征在于：所述改性胶原在医疗领域的应用。
CN201810697806.9A 2018-06-29 2018-06-29 一种聚乙二醇改性胶原膜及其应用 Pending CN108822318A (zh)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113527747A (zh) * 2021-07-30 2021-10-22 华南理工大学 一种改性的胶原膜及其制备方法、用于对胶原改性的活化多炔基交联剂

Citations (1)

* Cited by examiner, † Cited by third party
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CN106390195A (zh) * 2016-11-16 2017-02-15 四川大学 一种胶原膜的改性方法

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CN106390195A (zh) * 2016-11-16 2017-02-15 四川大学 一种胶原膜的改性方法

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HIROKI MAEDA等: "Effects of a polysaccharide nanogel‐crosslinked membrane on wound healing", 《JOURNAL OF BIOMEDICAL MATERIALS RESEARCH》 *
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113527747A (zh) * 2021-07-30 2021-10-22 华南理工大学 一种改性的胶原膜及其制备方法、用于对胶原改性的活化多炔基交联剂

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