CN108822182B - Preparation method of rhizoma paridis saponin H and application thereof in treating gastroenteritis - Google Patents

Preparation method of rhizoma paridis saponin H and application thereof in treating gastroenteritis Download PDF

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CN108822182B
CN108822182B CN201810813634.7A CN201810813634A CN108822182B CN 108822182 B CN108822182 B CN 108822182B CN 201810813634 A CN201810813634 A CN 201810813634A CN 108822182 B CN108822182 B CN 108822182B
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saponin
paris polyphylla
rhizoma paridis
silica gel
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CN108822182A (en
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夏祥华
李小勇
赵立春
乔丽娅
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Guangxi Botanical Garden of Medicinal Plants
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Abstract

The invention provides a preparation method of rhizoma paridis saponin H, which comprises the following steps: s1, weighing coarse powder of rhizoma paridis, adding cellulase and buffer solution, heating in water bath, performing enzymolysis, inactivating at high temperature, extracting with semi-bionic extractive solution at 37 deg.C under the assistance of ultrasonic wave, cooling, filtering, heating for concentrating, and mixing with silica gel to obtain silica gel powder of rhizoma paridis; s2, applying the paris polyphylla silica gel powder to a silica gel column, carrying out gradient elution by using a mixed solution of chloroform and methanol to obtain an eluted product, carrying out thin-layer chromatography analysis, combining the same phases to obtain a paris polyphylla saponin H crude sample, and carrying out reduced pressure concentration to obtain a paris polyphylla saponin H concentrated crude sample; s3, loading the concentrated crude rhizoma paridis saponin H sample on a reverse phase silica gel column, performing gradient elution with methanol solution, performing thin layer chromatography, combining the phases, degreasing with petroleum ether, extracting with ethyl acetate-butyl acetate, and concentrating under reduced pressure to obtain rhizoma paridis saponin H. The prepared rhizoma paridis saponin H has obvious effect when being applied to treating gastroenteritis.

Description

Preparation method of rhizoma paridis saponin H and application thereof in treating gastroenteritis
Technical Field
The invention relates to the technical field of traditional Chinese medicine pharmacy, in particular to a preparation method of rhizoma paridis saponin H and application thereof in treating gastroenteritis.
Background
Gastroenteritis is inflammation of the gastric and intestinal mucosa, and is caused by food poisoning. Gastroenteritis is a common and frequently encountered disease in summer and autumn. It is caused by infection of bacteria and viruses. Mainly manifested as diarrhea and abdominal discomfort with symptoms and degrees of the upper digestive tract, followed by electrolyte and fluid loss, and belongs to the category of vomiting, abdominal pain, diarrhea and other symptoms in traditional Chinese medicine
The rhizomes of multiple plants of Paris of Liliaceae (liliaceae) have the effects of clearing away heat and toxic materials, relieving swelling and pain, cooling liver and arresting convulsion, and have pharmacological activities of resisting tumor, stopping bleeding, resisting inflammation and the like in modern pharmacological research, wherein the active ingredient of the rhizomes is the total saponins of Paris. Mainly distributed in Yunnan, Guangxi, Sichuan and other places in China. Xiaomeifang et al investigated the effect of the paris saponin I on the proliferation and apoptosis of human liver cancer SMMC-7721 cell line in vitro and the related mechanism. Research shows that the paris polyphylla saponin I can inhibit the proliferation of the liver cancer SMMC-7721 cells in a time and concentration dependent manner, and probably the paris polyphylla saponin I inhibits the proliferation of the liver cancer cells by retarding the growth of tumor cells, inducing apoptosis and other mechanisms. The research on life science, 2011, 15 (6): 519)50 mu mol/L of a paris saponin II solution can inhibit local immunoreaction of the lupus nephritis in vitro, and can regulate Th1/Th2 imbalance by improving the expression of TGF-beta protein and IL-10 protein in lymphocytes so as to improve the immunosuppressive effect of CD4+ CD25+ Treg (Wang Juan and the like, the influence of the paris saponin II on cytokines expressed by peripheral blood CD4+ CD25+ T regulatory cells of patients with the lupus nephritis, 2010, 10 (1): 50).
Parg (paris polyphylla saponin H (pennogenin-3-O- [ alpha-L-arabinofuronase (1 → 4) ] -alpha-L-rhamnopyranosyl- (1 → 2) -beta-D-glucopyranoside, PARG) is an important steroid saponin, is also one of the main effective components in paris polyphylla, and has definite activities of stopping bleeding, diminishing inflammation and treating gastroenteritis. The paris polyphylla preparation gong xuening (containing paris polyphylla saponin H) can effectively treat various uterine bleeding diseases, the cure rate is 95.3 percent, and the treatment effect is more and more obvious along with the increase of the dosage. However, at present, the application of the paris polyphylla saponin H to the medicines for treating gastroenteritis is only rarely reported. In the aspect of extracting the rhizoma paridis saponin, the extraction of the rhizoma paridis total saponin or a mixture of several saponins is mainly used at present, the effective components are uncertain, and the problems of complicated extraction technology, low efficiency, low purity and the like exist.
Miao medicine rhizoma paridis and elecampane, Tibetan medicine fructus genkwa and cyanide have obvious effect in treating gastroenteritis, and the traditional Chinese medicine composition prepared by compounding the Miao medicine rhizoma paridis and elecampane and the Tibetan medicine fructus genkwa and the cyanide has good curative effect in treating acute and chronic gastroenteritis. Wherein, the paris polyphylla has better effects of stopping bleeding and diminishing inflammation, the elecampane strengthens the spleen and stomach, the witch fruit and the cyanide salt have the effects of diminishing inflammation, promoting gastric motility and enhancing appetite by being matched with other Chinese herbal medicine compounds.
Disclosure of Invention
In order to solve the technical problems, the invention provides a preparation method of rhizoma paridis saponin H and application thereof in treating gastroenteritis, and aims to provide a preparation method of rhizoma paridis saponin H, wherein the rhizoma paridis saponin H obtained by enzymolysis and ultrasonic-assisted extraction of semi-bionic extract has high purity (more than 98%), high yield (90-95%), simple method, no need of harsh experimental conditions, low cost and easy realization of industrial application.
The invention provides a preparation method of rhizoma paridis saponin H, which comprises the following steps:
accurately weighing coarse powder of rhizoma paridis, adding a certain amount of cellulase, adding a certain volume of buffer solution, heating in a water bath at 50 ℃, carrying out enzymolysis for a certain time, then inactivating at high temperature for 5min, extracting with a semi-bionic extracting solution with pH of 6.0-7.5 at 37 ℃ under the assistance of ultrasonic waves, cooling, filtering, combining filtrates, heating and concentrating in a water bath at 50 ℃, and mixing with silica gel to obtain rhizoma paridis silica gel powder;
step two, applying the paris polyphylla silica gel powder prepared in the step one to a silica gel column, eluting the product by using a mixed solution of chloroform and methanol to obtain an eluted product, performing thin-layer chromatography on the obtained eluted product, observing and developing under ultraviolet light, comparing the obtained eluted product with a paris polyphylla saponin H standard sample, finding out and extracting a crude sample containing the paris polyphylla saponin H, combining the same phases to obtain a paris polyphylla saponin H crude sample, and concentrating under reduced pressure to obtain a paris polyphylla saponin H concentrated crude sample;
and step three, loading the concentrated crude paris polyphylla saponin H sample prepared in the step three onto a reverse phase silica gel column, eluting the concentrated crude paris polyphylla saponin H sample by using a methanol solution, continuously performing thin layer chromatography, observing and developing color under ultraviolet light, comparing the color with the standard paris polyphylla saponin H sample, combining samples which have the same spots as the standard paris polyphylla saponin H sample and have no other impurities under fluorescence observation to obtain the methanol solution containing the purified product of the paris polyphylla saponin H, degreasing the solution by using petroleum ether, extracting by using ethyl acetate-butyl acetate, and removing the solvent by decompression concentration to obtain the paris polyphylla saponin H.
As a further improvement of the invention, the purity of the paris polyphylla saponin H is more than 98 percent, and the yield of the paris polyphylla saponin H is 90 to 95 percent.
As a further improvement of the invention, the semi-bionic extracting solution is prepared by 0.2 percent of sodium chloride, 0.32 percent of pepsin and 0.02mol/L of hydrochloric acid.
As a further improvement of the invention, in the first step, the buffer solution is NaAc-HAc buffer solution with pH 4.5; the grain number of the rhizoma paridis coarse powder is between 40 and 60 meshes; the solid-liquid ratio of the rhizoma paridis coarse powder to the buffer solution is 1: (15-25).
As a further improvement of the invention, in the step one, the enzymolysis time is 100-150 min; the enzyme dosage is (20-35) U/g substrate.
As a further improvement of the invention, in the first step, the silica gel is 100-200 meshes.
As a further improvement of the invention, in the step one, the ultrasonic power is 500-700W, and the ultrasonic time is 30-60 min.
As a further improvement of the invention, in the second step, the silica gel is 200-300 meshes; the elution is gradient elution; the ratio of chloroform to methanol is 100:1, 30:1, 20:1, 10:1 and 1:1 in sequence.
As a further improvement of the invention, the elution in the third step is gradient elution; the mass fractions of the methanol solution are 75%, 85% and 100% in sequence, and the volume ratio of the ethyl acetate to the butyl acetate is (1-5): 1.
as a further improvement of the invention, the ultraviolet light in step two and step three is 254 nm.
The invention further provides a traditional Chinese medicine composition for treating gastroenteritis, which is prepared from the following raw materials in parts by weight: 25 parts of rhizoma paridis saponin H; 22 parts of elecampane inula root; 17 parts of green leaf lopanadium gracile; 15 parts of mussaenda; 15 parts of cuttlebone; 13 parts of polygonatum; 12 parts of lilac daphne fruit; 10 parts of cyanide salt; 10 parts of satsuma orange; 7 parts of semen plantaginis; 6 parts of rhubarb; 5 parts of corn stigma.
The invention further provides a preparation method of the traditional Chinese medicine composition for treating gastroenteritis, which comprises the following steps:
drying elecampane, green leaf filigree, datura flower, cuttlebone, polygonatum odoratum, witch fruit, sal ammoniac, sand orange, plantain seed, rhubarb and corn stigma in the sun, crushing, mixing, adding water with the volume of 5-10 times of that of the mixture, soaking for 1-1.5h, decocting for 100-200min, filtering under reduced pressure, and reserving filtrate for later use;
step two, adding 3-6 times of volume of water into the dregs obtained in the step one, decocting for 60-120min, filtering under reduced pressure, and reserving filtrate for later use;
and step three, combining the filtrates obtained in the step one and the step two, sieving the filtrates by a 400-mesh sieve, adding the paris polyphylla saponin H, uniformly stirring the filtrates, concentrating the filtrate to the relative density of 1.2-1.7, adding auxiliary materials, and preparing the filtrate into a conventional preparation.
As a further improvement of the invention.
The invention further protects the use of one of the above.
The invention has the following beneficial effects:
1. the prepared paris polyphylla saponin H has high purity (more than 98 percent), high yield, simple and convenient method, low cost and easy realization of industrial application, and does not need harsh experimental conditions;
2. the rhizoma paridis saponin H has the activity of clearly stopping bleeding and diminishing inflammation and treating gastroenteritis, can dispel cold and promote digestion, remove blood stasis and eliminate stagnation, is used for treating chronic gastroenteritis and gastrorrhagia, is compounded with elecampane, Chinese caterpillar fungus, datura flower, cuttlebone, polygonatum, wuguo fruit, sal ammoniac, satsuma orange, plantain seed, rheum officinale and corn stigma, and has good treatment effects on diseases such as acute and chronic gastroenteritis, gastralgia, dyspepsia, inappetence, vomiting and diarrhea, fever and the like;
3. the components of the traditional Chinese medicine composition are all natural traditional Chinese medicine raw materials, and the traditional Chinese medicine composition is simple and convenient to prepare, wide in medicine source, obvious in gastroenteritis treatment effect, short in treatment course, low in cost and free of toxic and side effects.
Drawings
FIG. 1 is a diagram of the preparation process of rhizoma paridis saponin H;
FIG. 2 is a liquid chromatogram of Paris Saponin H.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention, and it is obvious that the embodiments described are only some representative embodiments of the present invention, rather than all embodiments, and all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
Example 1 preparation of Paris polyphylla Saponin H
The preparation method comprises the following steps:
accurately weighing 40-mesh coarse powder of rhizoma paridis, adding 20U/g substrate cellulase, adding NaAc-HAc buffer solution with pH of 4.5 (solid-liquid ratio is 1:15), heating in water bath at 50 ℃, performing enzymolysis for 100min, then performing high-temperature inactivation for 5min, extracting with semi-bionic extract (prepared from 0.2% sodium chloride, 0.32% pepsin and 0.02mol/L hydrochloric acid) with pH of 6.0 at 37 ℃ for 30min under the assistance of 500W ultrasonic waves, cooling, filtering, combining filtrates, heating and concentrating in water bath at 50 ℃, mixing with 100-mesh 200-mesh silica gel to obtain rhizoma paridis silica gel powder;
step two, applying the paris polyphylla silica gel powder prepared in the step one to a silica gel column (the silica gel is 200-300 meshes), performing gradient elution on the product by using a mixed solution of chloroform and methanol, wherein the ratio of the chloroform to the methanol is 100:1, 30:1, 20:1, 10:1 and 1:1 in sequence to obtain an eluted product, performing thin-layer chromatography on the obtained eluted product, observing and developing under ultraviolet light (254nm), comparing the obtained product with a standard sample of paris polyphylla saponin H, finding out and extracting a crude sample containing the paris polyphylla saponin H, combining the same phases to obtain a paris polyphylla saponin H crude sample, and performing reduced pressure concentration to obtain a paris polyphylla saponin H concentrated crude sample;
step three, loading the concentrated crude paris polyphylla saponin H sample prepared in the step three onto a reverse phase silica gel column, carrying out gradient elution on the concentrated crude paris polyphylla saponin H sample by using a methanol solution, wherein the mass fractions of the methanol solution are 75%, 85% and 100% in sequence, continuously carrying out thin-layer chromatography, observing and developing under ultraviolet light (254nm), comparing the color with a standard paris polyphylla saponin H sample, combining samples which have the same spots as the standard paris polyphylla saponin H sample and have no other impurities under fluorescence observation to obtain a methanol solution containing a purified product of the paris polyphylla saponin H, degreasing the methanol solution by using petroleum ether, extracting by using ethyl acetate-butyl acetate (the volume ratio is 1:1), and carrying out reduced pressure concentration to remove a solvent to obtain the paris polyphylla saponin H.
Example 2 preparation of Paris polyphylla Saponin H
The preparation method comprises the following steps:
accurately weighing 60-mesh coarse powder of rhizoma paridis, adding 35U/g substrate cellulase, adding NaAc-HAc buffer solution with pH of 4.5 (solid-liquid ratio is 1:25), heating in water bath at 50 ℃, performing enzymolysis for 150min, then performing high-temperature inactivation for 5min, extracting with semi-bionic extract (prepared from 0.2% sodium chloride, 0.32% pepsin and 0.02mol/L hydrochloric acid) with pH of 7.5 at 37 ℃ for 60min under the assistance of 700W ultrasonic waves, cooling, filtering, combining filtrates, heating and concentrating in water bath at 50 ℃, mixing with 100-mesh 200-mesh silica gel to obtain rhizoma paridis silica gel powder;
step two, applying the paris polyphylla silica gel powder prepared in the step one to a silica gel column (the silica gel is 200-300 meshes), performing gradient elution on the product by using a mixed solution of chloroform and methanol, wherein the ratio of the chloroform to the methanol is 100:1, 30:1, 20:1, 10:1 and 1:1 in sequence to obtain an eluted product, performing thin-layer chromatography on the obtained eluted product, observing and developing under ultraviolet light (254nm), comparing the obtained product with a standard sample of paris polyphylla saponin H, finding out and extracting a crude sample containing the paris polyphylla saponin H, combining the same phases to obtain a paris polyphylla saponin H crude sample, and performing reduced pressure concentration to obtain a paris polyphylla saponin H concentrated crude sample;
step three, loading the concentrated crude paris polyphylla saponin H sample prepared in the step three onto a reverse phase silica gel column, carrying out gradient elution on the concentrated crude paris polyphylla saponin H sample by using a methanol solution, wherein the mass fractions of the methanol solution are 75%, 85% and 100% in sequence, continuously carrying out thin-layer chromatography, observing and developing under ultraviolet light (254nm), comparing the color with a standard paris polyphylla saponin H sample, combining samples which have the same spots as the standard paris polyphylla saponin H sample and have no other impurities under fluorescence observation to obtain a methanol solution containing a purified product of the paris polyphylla saponin H, degreasing the methanol solution by using petroleum ether, extracting by using ethyl acetate-butyl acetate (the volume ratio is 5:1), and carrying out reduced pressure concentration to remove a solvent to obtain the paris polyphylla saponin H.
Example 3 preparation of Paris polyphylla Saponin H
The preparation method comprises the following steps:
accurately weighing 40-mesh coarse powder of rhizoma paridis, adding 30U/g cellulase as a substrate, adding NaAc-HAc buffer solution with pH of 4.5 (the solid-liquid ratio is 1:15), heating in water bath at 50 ℃, performing enzymolysis for 120min, then performing high-temperature inactivation for 5min, extracting with semi-bionic extract (prepared from 0.2% sodium chloride, 0.32% pepsin and 0.02mol/L hydrochloric acid) with pH of 6.5 at 37 ℃ for 50min under the assistance of 600W ultrasonic waves, cooling, filtering, combining filtrates, heating and concentrating in water bath at 50 ℃, mixing with 100-mesh 200-mesh silica gel to obtain rhizoma paridis silica gel powder;
step two, applying the paris polyphylla silica gel powder prepared in the step one to a silica gel column (the silica gel is 200-300 meshes), performing gradient elution on the product by using a mixed solution of chloroform and methanol, wherein the ratio of the chloroform to the methanol is 100:1, 30:1, 20:1, 10:1 and 1:1 in sequence to obtain an eluted product, performing thin-layer chromatography on the obtained eluted product, observing and developing under ultraviolet light (254nm), comparing the obtained product with a standard sample of paris polyphylla saponin H, finding out and extracting a crude sample containing the paris polyphylla saponin H, combining the same phases to obtain a paris polyphylla saponin H crude sample, and performing reduced pressure concentration to obtain a paris polyphylla saponin H concentrated crude sample;
step three, loading the concentrated crude paris polyphylla saponin H sample prepared in the step three onto a reverse phase silica gel column, carrying out gradient elution on the concentrated crude paris polyphylla saponin H sample by using a methanol solution, wherein the mass fractions of the methanol solution are 75%, 85% and 100% in sequence, continuously carrying out thin-layer chromatography, observing and developing under ultraviolet light (254nm), comparing the color with a standard paris polyphylla saponin H sample, combining samples which have the same spots as the standard paris polyphylla saponin H sample and have no other impurities under fluorescence observation to obtain a methanol solution containing a purified product of the paris polyphylla saponin H, degreasing the methanol solution by using petroleum ether, extracting by using ethyl acetate-butyl acetate (the volume ratio is 3:1), and carrying out reduced pressure concentration to remove a solvent to obtain the paris polyphylla saponin H.
Example 4 preparation of a Chinese medicinal composition for the treatment of gastroenteritis
The raw materials comprise:
25 parts of rhizoma paridis saponin H; 22 parts of elecampane inula root; 17 parts of green leaf lopanadium gracile; 15 parts of mussaenda; 15 parts of cuttlebone; 13 parts of polygonatum; 12 parts of lilac daphne fruit; 10 parts of cyanide salt; 10 parts of satsuma orange; 7 parts of semen plantaginis; 6 parts of rhubarb; 5 parts of corn stigma.
The preparation method comprises the following steps:
drying elecampane, Chinese silverweed herb, datura flower, cuttlebone, polygonatum, witch fruit, sal ammoniac, sand orange, plantain seed, rhubarb and corn stigma in the sun, grinding, mixing, adding 5-fold volume of water, soaking for 1h, decocting for 100min, filtering under reduced pressure, and obtaining filtrate for later use;
step two, adding 3-fold water into the dregs obtained in the step one, decocting for 60min, filtering under reduced pressure, and keeping the filtrate for later use;
and step three, combining the filtrates obtained in the step one and the step two, sieving the filtrates by a 400-mesh sieve, adding the paris polyphylla saponin H, uniformly stirring the filtrates, concentrating the filtrate to the relative density of 1.7, adding maltodextrin, and performing spray drying to prepare solid medicinal powder.
Example 4 preparation of a Chinese medicinal composition for treating gastroenteritis containing no Paris Saponin H
The raw materials comprise:
22 parts of elecampane inula root; 17 parts of green leaf lopanadium gracile; 15 parts of mussaenda; 15 parts of cuttlebone; 13 parts of polygonatum; 12 parts of lilac daphne fruit; 10 parts of cyanide salt; 10 parts of satsuma orange; 7 parts of semen plantaginis; 6 parts of rhubarb; 5 parts of corn stigma.
The preparation method comprises the following steps:
drying elecampane, Chinese silverweed herb, datura flower, cuttlebone, polygonatum, witch fruit, sal ammoniac, sand orange, plantain seed, rhubarb and corn stigma in the sun, grinding, mixing, adding 10-fold volume of water, soaking for 1.5h, decocting for 200min, filtering under reduced pressure, and reserving filtrate for later use;
step two, adding 6-fold water into the dregs obtained in the step one, decocting for 120min, filtering under reduced pressure, and reserving filtrate for later use;
and step three, combining the filtrates obtained in the step one and the step two, sieving the filtrates by a 400-mesh sieve, uniformly stirring, concentrating the filtrate to the relative density of 1.2, adding maltodextrin, and performing spray drying to prepare the solid medicine powder without the paris polyphylla saponin H.
Test example 1 identification of Paris polyphylla Saponin H
The paris polyphylla saponin H prepared in example 1 is placed in deuterated chloroform for nuclear magnetic resonance wave-front detection, and the result is as follows: ESI-MS gives the excimer ion peak m/z: 869[ M-H ]]-; the molecular formula is C by combining 1H NMR and 13C NMR44H70O17。
1H NMR(DMSO-d6,400MHz)δ：0.79(3H,d,J＝7.2Hz，H-27)，0.73(3H,s,H-18)，0.95(3H,s,H-19),1.09(3H,d,J＝6.0Hz,H-21),1.15(3H,d,J-6.2Hz,rhaH-6)，3.21(2H，m,H-26),3.66(1H,m,H-3),4.43(1H,d,J-8.0。Hz,gluH-1),4.88(1H,d,J＝1.7Hz，araH-1),5.03(1H,d,J＝1.2Hz,rhaH-1),5.34(1H,br,H-6)。
13C NMR identification results show that chemical shifts of 1-27 of the glycoside ligand position are as follows: 37.2, 30.0, 76.7, 38.9, 140.6, 121.8, 31.9, 31.2, 49.9, 36.7, 20.4, 31.944.9, 52.3, 31.9, 89.4, 89.3, 17.0, 19.3, 44.0, 9.7, 109.1, 31.7, 28.4, 29.4, 66.3, 17.5; the chemical shifts of 1-20 positions of sugar-containing position are as follows: 98.4, 76.6, 76.2, 75.2, 76.2, 61.6, Rha2, 100.5, 70.8, 68.3, 72.1, 66.2, 18.1, Ara3, 108.1, 81.6, 76.8, 84.6, 60.3. The compound was hydrolyzed in situ and glucose, rhamnose and arabinose were detected on TLC.
Further in combination with literature reports, compound 5 was determined to have the structure: the molecular structural formula of the paris polyphylla saponin H is pennogenin-3-O- [ alpha-L-arabinofuroside (1 → 4) ] -alpha-L-rhamnopyranosyl- (1 → 2) -beta-D-glucopyranoside.
Test example 2 purity test of Paris polyphylla Saponin H
Loading the obtained rhizoma paridis saponin H of example 1 into high performance liquid chromatography under Inertsil ODS-3C18 chromatographic column (150mm × 4.6mm, 5 μm); the mobile phase is acetonitrile-water (50: 50); the detection wavelength is 203 nm; the flow rate is 1.0mL min < -1 >; the column temperature was 35 ℃; the amount of the sample was 20.0. mu.L.
The purity of rhizoma paridis saponin H calculated by area normalization method is greater than 98.5%, and the liquid chromatogram is shown in figure 2. The invention provides a new idea for extracting the paris polyphylla saponin H from the paris polyphylla.
Test example 3 clinical test
1. And (4) experimental groups. Is divided into 5 groups, i.e. rhizoma paridis saponin H treatment group (1 group), Chinese medicinal composition treatment group (2 group), rhizoma paridis saponin H-free Chinese medicinal composition treatment group (3 group), omeprazole treatment group (control group) and blank group.
2. Experimental methods.
(1) And (4) case selection. 100 outpatients collected in 2017-2018 in 2 months of a first affiliated hospital of traditional Chinese medicine university in Guangxi province are taken, and 20 outpatients are taken in each group. Group 1: 10 male cases, 10 female cases, 22-56 years old, 37 years old on average, 1-20 months old, 6 vomiting cases, 6 diarrhea and abdominal pain cases, and 8 fever cases; and 2, group: 11 male cases, 9 female cases, 20-53 years old, 35 years old on average, 3-20 months old, 7 vomiting cases, 4 diarrhea and abdominal pain cases, and 9 fever cases; and 3, group: 8 male cases, 12 female cases, 21-57 years old, 36 years old on average, 2-20 months old, 5 vomiting cases, 6 diarrhea and abdominal pain cases, and 9 fever cases; control group: 10 male cases, 10 female cases, age 19-57 years, average age 36 years, course of disease 2-22 months, wherein 8 cases of vomiting, 6 cases of diarrhea and abdominal pain, and 6 cases of fever; blank group: 9 men and 11 women, age 20-57 years, average age 35 years, course of disease 3-20 months, wherein 6 cases of vomiting, 8 cases of diarrhea and abdominal pain, and 6 cases of fever.
(2) Methods of treatment. 1 group of patients orally take 8-10 g of the paris polyphylla saponin H prepared in example 1 every time, 3 times a day; 2 groups of patients take the traditional Chinese medicine composition prepared in the embodiment 4 orally, 8-10 g each time, 3 times a day; 3 groups of patients take the traditional Chinese medicine composition of the paris polyphylla saponin H prepared in the embodiment 5 orally, 8-10 g each time, 3 times a day; omeprazole granules are orally taken by patients in a control group 8-10 g each time and 3 times a day, and placebo (mainly prepared from starch and the like) is orally taken by patients in a blank group 8-10 g each time and 3 times a day.
(3) And (5) observing indexes. The clinical treatment effect of group 5 was compared with the improvement time of clinical symptoms such as abdominal pain, diarrhea, fever, and vomiting.
(4) The curative effect is standard. The effect is shown: the clinical symptoms of the patient are obviously improved and tend to be normal, and the symptoms of diarrhea, abdominal pain, vomiting, fever and the like are obviously improved. The method has the following advantages: the symptoms of the patients are obviously improved, and the times and the amount of symptoms such as diarrhea, abdominal pain, vomiting, fever and the like are obviously improved. And (4) invalidation: all the above standards are not met.
Total effective is effective plus effective
The results are shown in Table 1.
TABLE 1 comparison of the post-treatment efficacy of five groups of patients
Group of n Show effect Is effective Invalidation Total effective (%)
Group 1 20 8 4 8 60
2 groups of 20 14 5 1 95
Group 3 20 8 5 7 65
Control group 20 11 2 7 65
Blank group 20 0 1 19 5
From the above results, 1 group, 2 groups, 3 groups and the control group all have the effect of treating gastroenteritis, and the traditional Chinese medicine composition prepared in example 4 can obviously improve clinical symptoms such as abdominal pain, diarrhea, fever, vomiting and the like, and the total effective rate reaches 95%. The effect of the paris polyphylla saponin H is not as good as that of omeprazole used as a medicine for treating gastroenteritis when the paris polyphylla saponin H is used alone, but the treatment effect of the traditional Chinese medicine composition can be obviously enhanced by adding the paris polyphylla saponin H into the traditional Chinese medicine composition, and the traditional Chinese medicine composition has good clinical application prospect.
Various modifications may be made to the above without departing from the spirit and scope of the invention as defined by the appended claims. The scope of the invention is therefore intended to be limited not by the above description, but rather by the scope of the appended claims.

Claims (10)

1. The preparation method of the paris polyphylla saponin H is characterized by comprising the following steps:
accurately weighing coarse powder of rhizoma paridis, adding a certain amount of cellulase, adding a certain volume of buffer solution, heating in a water bath at 50 ℃, carrying out enzymolysis for a certain time, then inactivating at high temperature for 5min, extracting with a semi-bionic extracting solution with pH of 6.0-7.5 at 37 ℃ under the assistance of ultrasonic waves, cooling, filtering, combining filtrates, heating and concentrating in a water bath at 50 ℃, and mixing with silica gel to obtain rhizoma paridis silica gel powder;
step two, applying the paris polyphylla silica gel powder prepared in the step one to a silica gel column, eluting the product by using a mixed solution of chloroform and methanol to obtain an eluted product, performing thin-layer chromatography on the obtained eluted product, observing and developing under ultraviolet light, comparing the obtained eluted product with a paris polyphylla saponin H standard sample, finding out and extracting a crude sample containing the paris polyphylla saponin H, combining the same phases to obtain a paris polyphylla saponin H crude sample, and concentrating under reduced pressure to obtain a paris polyphylla saponin H concentrated crude sample;
and step three, loading the concentrated crude paris polyphylla saponin H sample prepared in the step two on a reverse phase silica gel column, eluting the concentrated crude paris polyphylla saponin H sample by using a methanol solution, continuously performing thin layer chromatography, observing and developing color under ultraviolet light, comparing the color with the standard paris polyphylla saponin H sample, combining samples which have the same spots as the standard paris polyphylla saponin H sample and have no other impurities under fluorescence observation to obtain the methanol solution containing the purified product of the paris polyphylla saponin H, degreasing the solution by using petroleum ether, extracting by using ethyl acetate-butyl acetate, and removing the solvent by decompression concentration to obtain the paris polyphylla saponin H.
2. The method for preparing the paris polyphylla saponin H according to claim 1, wherein the purity of the paris polyphylla saponin H is more than 98%, and the yield of the paris polyphylla saponin H is 90-95%.
3. The method for preparing paris polyphylla saponin H according to claim 1, wherein the semi-bionic extracting solution is prepared from 0.2% of sodium chloride, 0.32% of pepsin and 0.02mol/L of hydrochloric acid.
4. The method for preparing paris polyphylla saponin H according to claim 1, wherein the buffer solution in step one is NaAc-HAc buffer solution with pH 4.5; the grain size number of the rhizoma paridis coarse powder is between 40 and 60 meshes; the solid-liquid ratio of the rhizoma paridis coarse powder to the buffer solution is 1: (15-25).
5. The method for preparing rhizoma paridis saponin H as claimed in claim 1, wherein the enzymolysis time in step one is 100-; the enzyme dosage is (20-35) U/g substrate.
6. The method as claimed in claim 1, wherein the silica gel is 100-200 mesh in the first step.
7. The method for preparing rhizoma paridis saponin H as claimed in claim 1, wherein the ultrasonic power is 500-.
8. The method for preparing rhizoma paridis saponin H as claimed in claim 1, wherein the silica gel in step two is 200-300 mesh; the elution is gradient elution; the ratio of chloroform to methanol is 100:1, 30:1, 20:1, 10:1 and 1:1 in sequence.
9. The method for preparing rhizoma paridis saponin H according to claim 1, wherein the elution in step three is gradient elution; the mass fractions of the methanol solution are 75%, 85% and 100% in sequence, and the volume ratio of the ethyl acetate to the butyl acetate is (1-5): 1.
10. the method for preparing paris polyphylla saponin H according to claim 1, wherein the ultraviolet light in step two and step three is 254 nm.
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