CN108821384A - The microparticle polyurethane rubber carrier of fixed denitrifying bacterium - Google Patents
The microparticle polyurethane rubber carrier of fixed denitrifying bacterium Download PDFInfo
- Publication number
- CN108821384A CN108821384A CN201810766775.8A CN201810766775A CN108821384A CN 108821384 A CN108821384 A CN 108821384A CN 201810766775 A CN201810766775 A CN 201810766775A CN 108821384 A CN108821384 A CN 108821384A
- Authority
- CN
- China
- Prior art keywords
- mixed liquor
- substance
- minutes
- added
- obtains
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/28—Treatment of water, waste water, or sewage by sorption
- C02F1/285—Treatment of water, waste water, or sewage by sorption using synthetic organic sorbents
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
- C02F3/2806—Anaerobic processes using solid supports for microorganisms
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
- C02F2101/163—Nitrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Water Treatment By Sorption (AREA)
Abstract
The invention discloses a kind of microparticle polyurethane rubber carriers of fixed denitrifying bacterium.The mixed liquor prepared after polyurethane rubber particle cleaning through chitosan, beta-cyclodextrin modified is obtained into substance C；The mixed liquor that substance C is prepared through 4-propyl bromide, tetrabutyl ammonium hydrogen phosphate and etamon chloride is modified to obtain substance D；The microparticle polyurethane rubber carrier for air-drying and fixed denitrifying bacterium can be obtained is taken out after fixing denitrifying bacterium on substance D.The invention has the advantages that the carrier of preparation can adsorb nitrate, while having many advantages, such as that denitrification denitrogenation effect is good.
Description
Technical field
The invention belongs to water body in lake reparation and Treatment process field, in particular to a kind of microparticle of fixed denitrifying bacterium
Polyurethane rubber carrier.
Background technique
Water body in lake eutrophication has become one of main Environmental Problems generally existing both at home and abroad at present.Water body in lake
Have the characteristics that poor fluidity, self-purification capacity are poor, be easily contaminated, after there is eutrophication, algae and water can be a large amount of
Phenomena such as breeding, color is green tinged, will appear when serious and distributes stench, and aquatile is dead, loses landscape ability, seriously affects week
The living environment of the natural environment and resident that enclose.It is biological floating bed using modified high molecular material as carrier or matrix, will be aquatic
Plant is planted in the bed body for floating on the nature water surface by the happiness land and water plant etc. of improvement, adsorbed using carrier material,
The effects of absorption, retention and absorption of root system of plant, removes the polluters such as Water phosphorus.Biological floating bed plant is growing
The root system of plant of large area is generated in the process, and root system of plant is intricate, and can form one of filter layer, when water body is from plant
Root system when passing through, some large particles and polluter in water body can be intercepted by the filter layer, reach purification
The purpose of water body.In addition, the growth of plant needs the nutriments such as nitrogen phosphorus, and excessive nitrogen phosphorus can satisfy plant in polluted-water
Object growth needs, eventually by being absorbed and utilized for plant is achieved the purpose that remove water body in excess nitrogen phosphorus.It is floating in ecology
There are the microorganisms such as a large amount of bacterium, fungi, protozoan, these microorganism mass propagations to form biology on the surface of bed carrier
Film is removed the pollutant in water body by the metabolic activity of microorganism.Plant can pass through the gas of branch and root system
Transmission and release action, the oxygen transmission in oxygen or atmosphere that photosynthesis is generated to root system, a part are carried out for plant
Endogenous respiration effect, another part can be then diffused into around root system in the environment of anoxic by the oxygen concentration difference in water body,
To which there are aerobic zones and anaerobic zone in biological floating bed bottom water body, movable item is provided for different types of microorganism
Part.The plant of biological floating bed upper plantation nutriment, in terms of have apparent competitive advantage to algae, can play bright
Aobvious inhibiting effect；Plant can secret out of certain biochemical substances into water body during the growth process simultaneously, these substances can press down
The growth of algae processed is even killed；Some algophagous meiofaunas can be inhabited on the root system of plant, can more be limited
The growth of algae processed.Biological floating bed technology has simple facility, small investment, easily builds, is convenient for management, beautifying the environment, suitable kind of model
The advantages that wide is enclosed, is had broad application prospects.Also lack the microparticle polyurethane rubber carrier of fixed denitrifying bacterium at present, together
When, for the absorption property for enhancing biological floating bed carrier, need to develop novel carrier material.Meanwhile by biological floating bed building
Progress is combined with matched carrier material preparation, so that the synergistic effect of carrier material and plant is not fully exerted is
One of emphasis studied at present.It is combined currently, also lacking biological floating bed building with matched carrier material preparation
The research of aspect.
Summary of the invention
The object of the present invention is to provide a kind of microparticle polyurethane rubber carriers of fixed denitrifying bacterium, and the carrier is by as follows
Method preparation：
(1) the polyurethane rubber particle that 800 gram particle diameters are 1-2mm is added in 1000mL tap water, in 1000r/min
Under the conditions of stir 15 minutes, be filtered to remove liquid and obtain substance A, by substance A be added to mass percent concentration be 4.5% chlorine
Change in sodium solution, is stirred 15 minutes under the conditions of 1000r/min, be filtered to remove liquid and obtain substance B；
(2) 26.5 grams of chitosans are added in 4000mL deionized water, stir 3 minutes, shakes under the conditions of 1000r/min
It is divided into 4 parts of equivalent after even, obtains mixed liquor H1, mixed liquor H2, mixed liquor H3, mixed liquor H4；
(3) 3.8 grams of beta-cyclodextrins are added in mixed liquor H1, stir 3 minutes, is mixed under the conditions of 1000r/min
Close liquid J1；
(4) substance B is added in mixed liquor J1, is shaken 35 minutes in the shaking table that temperature is 40 DEG C, is filtered to remove liquid
Body obtains substance B 1, and substance B 1 is placed 120 minutes in the drying box that temperature is 55 DEG C, obtains substance B 2；
(5) 3.6 grams of beta-cyclodextrins are added in mixed liquor H2, stir 3 minutes, is mixed under the conditions of 1000r/min
Close liquid J2；
(6) substance B 2 is added in mixed liquor J2, is shaken 35 minutes in the shaking table that temperature is 40 DEG C, is filtered to remove liquid
Body obtains substance B 3, and substance B 3 is placed 120 minutes in the drying box that temperature is 55 DEG C, obtains substance B 4；
(7) 3.4 grams of beta-cyclodextrins are added in mixed liquor H3, stir 3 minutes, is mixed under the conditions of 1000r/min
Close liquid J3；
(8) substance B 4 is added in mixed liquor J3, is shaken 35 minutes in the shaking table that temperature is 40 DEG C, is filtered to remove liquid
Body obtains substance B 5, and substance B 5 is placed 120 minutes in the drying box that temperature is 55 DEG C, obtains substance B 6；
(9) 3.2 grams of beta-cyclodextrins are added in mixed liquor H4, stir 3 minutes, is mixed under the conditions of 1000r/min
Close liquid J4；
(10) substance B 6 is added in mixed liquor J4, shakes 35 minutes, is filtered to remove in the shaking table that temperature is 40 DEG C
Liquid obtains substance B 7, and substance B 7 is placed 120 minutes in the drying box that temperature is 55 DEG C, obtains substance C；
(11) 15.6 grams of 4-propyl bromides are added in 4000mL deionized water, stir 3 under the conditions of 1000r/min
Minute, 5 parts of equivalent are divided into after shaking up, obtains mixed liquor K1, mixed liquor K2, mixed liquor K3, mixed liquor K4, mixed liquor K5；
(12) 14.8 grams of tetrabutyl ammonium hydrogen phosphates are added in 4000mL deionized water, are stirred under the conditions of 1000r/min
It mixes 3 minutes, 5 parts of equivalent is divided into after shaking up, obtain mixed liquor L1, mixed liquor L2, mixed liquor L3, mixed liquor L4, mixed liquor L5；
(13) mixed liquor L1 and 2.6 grams of etamon chlorides are added in mixed liquor K1, are stirred under the conditions of 1000r/min
It mixes 3 minutes, obtains mixed liquor M1；
(14) substance C is added in mixed liquor M1, is shaken 35 minutes in the shaking table that temperature is 40 DEG C, is filtered to remove liquid
Body obtains substance C 1, and substance C 1 is placed 120 minutes in the drying box that temperature is 55 DEG C, obtains substance C 2；
(15) mixed liquor L2 and 2.9 grams of etamon chlorides are added in mixed liquor K2, are stirred under the conditions of 1000r/min
It mixes 3 minutes, obtains mixed liquor M2；
(16) substance C 2 is added in mixed liquor M2, shakes 35 minutes, is filtered to remove in the shaking table that temperature is 40 DEG C
Liquid obtains substance C 3, and substance C 3 is placed 120 minutes in the drying box that temperature is 55 DEG C, obtains substance C 4；
(17) mixed liquor L3 and 3.2 grams of etamon chlorides are added in mixed liquor K3, are stirred under the conditions of 1000r/min
It mixes 3 minutes, obtains mixed liquor M3；
(18) substance C 4 is added in mixed liquor M3, shakes 35 minutes, is filtered to remove in the shaking table that temperature is 40 DEG C
Liquid obtains substance C 5, and substance C 5 is placed 120 minutes in the drying box that temperature is 55 DEG C, obtains substance C 6；
(19) mixed liquor L4 and 3.5 grams of etamon chlorides are added in mixed liquor K4, are stirred under the conditions of 1000r/min
It mixes 3 minutes, obtains mixed liquor M4；
(20) substance C 6 is added in mixed liquor M4, shakes 35 minutes, is filtered to remove in the shaking table that temperature is 40 DEG C
Liquid obtains substance C 7, and substance C 7 is placed 120 minutes in the drying box that temperature is 55 DEG C, obtains substance C 8；
(21) mixed liquor L5 and 3.8 grams of etamon chlorides are added in mixed liquor K5, are stirred under the conditions of 1000r/min
It mixes 3 minutes, obtains mixed liquor M5；
(22) substance C 8 is added in mixed liquor M5, shakes 35 minutes, is filtered to remove in the shaking table that temperature is 40 DEG C
Liquid obtains substance C 9, and substance C 9 is placed 120 minutes in the drying box that temperature is 55 DEG C, obtains substance D；
(23) denitrifying bacterium system is added in 1000mL basic culture solution and obtains mixed liquor N, make denitrification in mixed liquor N
The bacteria containing amount of bacterium is 7.5 × 1010CFU/mL, wherein the group of basic culture solution becomes：NaNO3:2.5g/L, NaCl:7.5g/L
KH2PO4:3.5g/L, K2HPO4:3.0g/L, FeCl3:0.15g/L, MgSO4:3.0g/L, CaCl2·2H2O:0.3g/L；
(24) substance D is cultivated to take out afterwards for 24 hours in mixed liquor N and is air-dried, the microparticle of fixed denitrifying bacterium can be obtained
Polyurethane rubber carrier.
The invention has the advantages that the carrier of preparation can adsorb nitrate, while having denitrification denitrogenation effect good
The advantages that.
Specific embodiment
Embodiment
(1) the microparticle polyurethane rubber carrier of fixed denitrifying bacterium is prepared by the following method：
1) the polyurethane rubber particle that 800 gram particle diameters are 1-2mm is added in 1000mL tap water, in 1000r/min
Under the conditions of stir 15 minutes, be filtered to remove liquid and obtain substance A, by substance A be added to mass percent concentration be 4.5% chlorine
Change in sodium solution, is stirred 15 minutes under the conditions of 1000r/min, be filtered to remove liquid and obtain substance B；
2) 26.5 grams of chitosans are added in 4000mL deionized water, stir 3 minutes, shakes under the conditions of 1000r/min
It is divided into 4 parts of equivalent after even, obtains mixed liquor H1, mixed liquor H2, mixed liquor H3, mixed liquor H4；
3) 3.8 grams of beta-cyclodextrins are added in mixed liquor H1, stir 3 minutes, is mixed under the conditions of 1000r/min
Liquid J1；
4) substance B is added in mixed liquor J1, is shaken 35 minutes in the shaking table that temperature is 40 DEG C, is filtered to remove liquid
Substance B 1 is obtained, substance B 1 is placed 120 minutes in the drying box that temperature is 55 DEG C, obtains substance B 2；
5) 3.6 grams of beta-cyclodextrins are added in mixed liquor H2, stir 3 minutes, is mixed under the conditions of 1000r/min
Liquid J2；
6) substance B 2 is added in mixed liquor J2, is shaken 35 minutes in the shaking table that temperature is 40 DEG C, is filtered to remove liquid
Body obtains substance B 3, and substance B 3 is placed 120 minutes in the drying box that temperature is 55 DEG C, obtains substance B 4；
7) 3.4 grams of beta-cyclodextrins are added in mixed liquor H3, stir 3 minutes, is mixed under the conditions of 1000r/min
Liquid J3；
8) substance B 4 is added in mixed liquor J3, is shaken 35 minutes in the shaking table that temperature is 40 DEG C, is filtered to remove liquid
Body obtains substance B 5, and substance B 5 is placed 120 minutes in the drying box that temperature is 55 DEG C, obtains substance B 6；
9) 3.2 grams of beta-cyclodextrins are added in mixed liquor H4, stir 3 minutes, is mixed under the conditions of 1000r/min
Liquid J4；
10) substance B 6 is added in mixed liquor J4, is shaken 35 minutes in the shaking table that temperature is 40 DEG C, is filtered to remove liquid
Body obtains substance B 7, and substance B 7 is placed 120 minutes in the drying box that temperature is 55 DEG C, obtains substance C；
11) 15.6 grams of 4-propyl bromides are added in 4000mL deionized water, stir 3 under the conditions of 1000r/min
Minute, 5 parts of equivalent are divided into after shaking up, obtains mixed liquor K1, mixed liquor K2, mixed liquor K3, mixed liquor K4, mixed liquor K5；
12) 14.8 grams of tetrabutyl ammonium hydrogen phosphates are added in 4000mL deionized water, are stirred under the conditions of 1000r/min
3 minutes, 5 parts of equivalent are divided into after shaking up, obtains mixed liquor L1, mixed liquor L2, mixed liquor L3, mixed liquor L4, mixed liquor L5；
13) mixed liquor L1 and 2.6 grams of etamon chlorides are added in mixed liquor K1, are stirred under the conditions of 1000r/min
It mixes 3 minutes, obtains mixed liquor M1；
14) substance C is added in mixed liquor M1, is shaken 35 minutes in the shaking table that temperature is 40 DEG C, is filtered to remove liquid
Body obtains substance C 1, and substance C 1 is placed 120 minutes in the drying box that temperature is 55 DEG C, obtains substance C 2；
15) mixed liquor L2 and 2.9 grams of etamon chlorides are added in mixed liquor K2, are stirred under the conditions of 1000r/min
It mixes 3 minutes, obtains mixed liquor M2；
16) substance C 2 is added in mixed liquor M2, is shaken 35 minutes in the shaking table that temperature is 40 DEG C, is filtered to remove liquid
Body obtains substance C 3, and substance C 3 is placed 120 minutes in the drying box that temperature is 55 DEG C, obtains substance C 4；
17) mixed liquor L3 and 3.2 grams of etamon chlorides are added in mixed liquor K3, are stirred under the conditions of 1000r/min
It mixes 3 minutes, obtains mixed liquor M3；
18) substance C 4 is added in mixed liquor M3, is shaken 35 minutes in the shaking table that temperature is 40 DEG C, is filtered to remove liquid
Body obtains substance C 5, and substance C 5 is placed 120 minutes in the drying box that temperature is 55 DEG C, obtains substance C 6；
19) mixed liquor L4 and 3.5 grams of etamon chlorides are added in mixed liquor K4, are stirred under the conditions of 1000r/min
It mixes 3 minutes, obtains mixed liquor M4；
20) substance C 6 is added in mixed liquor M4, is shaken 35 minutes in the shaking table that temperature is 40 DEG C, is filtered to remove liquid
Body obtains substance C 7, and substance C 7 is placed 120 minutes in the drying box that temperature is 55 DEG C, obtains substance C 8；
21) mixed liquor L5 and 3.8 grams of etamon chlorides are added in mixed liquor K5, are stirred under the conditions of 1000r/min
It mixes 3 minutes, obtains mixed liquor M5；
22) substance C 8 is added in mixed liquor M5, is shaken 35 minutes in the shaking table that temperature is 40 DEG C, is filtered to remove liquid
Body obtains substance C 9, and substance C 9 is placed 120 minutes in the drying box that temperature is 55 DEG C, obtains substance D；
23) denitrifying bacterium system is added in 1000mL basic culture solution and obtains mixed liquor N, make denitrification in mixed liquor N
The bacteria containing amount of bacterium is 7.5 × 1010CFU/mL, wherein the group of basic culture solution becomes：NaNO3:2.5g/L, NaCl:7.5g/L
KH2PO4:3.5g/L, K2HPO4:3.0g/L, FeCl3:0.15g/L, MgSO4:3.0g/L, CaCl2·2H2O:0.3g/L；
24) substance D is cultivated to take out afterwards for 24 hours in mixed liquor N and is air-dried, the microparticle that fixed denitrifying bacterium can be obtained is poly-
Urethane rubber carrier.
(2) application experiment
Here is with fixing the microparticle polyurethane rubber carrier of denitrifying bacterium made from the method for the present invention to containing nitre
The water body of hydrochlorate pollution has carried out absorption and denitrification removal is tested, and further illustrates the present invention.
The microparticle polyurethane rubber carrier that denitrifying bacterium is fixed made from the method for the present invention is placed in azotate pollution
Water body in carried out absorption and denitrification removal test, the results showed that：2.8 are added into the water body of 1000mL azotate pollution
The microparticle polyurethane rubber carrier of gram fixed denitrifying bacterium, the concentration of nitrate is dropped by 36.8mg/L in water body after processing 3 days
As low as 1.1mg/L.
Claims (1)
1. a kind of microparticle polyurethane rubber carrier of fixed denitrifying bacterium, which is characterized in that the carrier is prepared by the following method：
(1) the polyurethane rubber particle that 800 gram particle diameters are 1-2mm is added in 1000mL tap water, in 1000r/min condition
Lower stirring 15 minutes, is filtered to remove liquid and obtains substance A, and substance A is added to the sodium chloride that mass percent concentration is 4.5%
In solution, is stirred 15 minutes under the conditions of 1000r/min, be filtered to remove liquid and obtain substance B；
(2) 26.5 grams of chitosans are added in 4000mL deionized water, are stirred 3 minutes under the conditions of 1000r/min, after shaking up
It is divided into 4 parts of equivalent, obtains mixed liquor H1, mixed liquor H2, mixed liquor H3, mixed liquor H4；
(3) 3.8 grams of beta-cyclodextrins are added in mixed liquor H1, are stirred 3 minutes under the conditions of 1000r/min, obtains mixed liquor
J1；
(4) substance B is added in mixed liquor J1, is shaken 35 minutes in the shaking table that temperature is 40 DEG C, is filtered to remove liquid and obtains
To substance B 1, substance B 1 is placed 120 minutes in the drying box that temperature is 55 DEG C, obtains substance B 2；
(5) 3.6 grams of beta-cyclodextrins are added in mixed liquor H2, are stirred 3 minutes under the conditions of 1000r/min, obtains mixed liquor
J2；
(6) substance B 2 is added in mixed liquor J2, is shaken 35 minutes in the shaking table that temperature is 40 DEG C, is filtered to remove liquid and obtains
To substance B 3, substance B 3 is placed 120 minutes in the drying box that temperature is 55 DEG C, obtains substance B 4；
(7) 3.4 grams of beta-cyclodextrins are added in mixed liquor H3, are stirred 3 minutes under the conditions of 1000r/min, obtains mixed liquor
J3；
(8) substance B 4 is added in mixed liquor J3, is shaken 35 minutes in the shaking table that temperature is 40 DEG C, is filtered to remove liquid and obtains
To substance B 5, substance B 5 is placed 120 minutes in the drying box that temperature is 55 DEG C, obtains substance B 6；
(9) 3.2 grams of beta-cyclodextrins are added in mixed liquor H4, are stirred 3 minutes under the conditions of 1000r/min, obtains mixed liquor
J4；
(10) substance B 6 is added in mixed liquor J4, is shaken 35 minutes in the shaking table that temperature is 40 DEG C, is filtered to remove liquid
Substance B 7 is obtained, substance B 7 is placed 120 minutes in the drying box that temperature is 55 DEG C, obtains substance C；
(11) 15.6 grams of 4-propyl bromides are added in 4000mL deionized water, 3 points is stirred under the conditions of 1000r/min
Clock is divided into 5 parts of equivalent after shaking up, obtain mixed liquor K1, mixed liquor K2, mixed liquor K3, mixed liquor K4, mixed liquor K5；
(12) 14.8 grams of tetrabutyl ammonium hydrogen phosphates are added in 4000mL deionized water, 3 points is stirred under the conditions of 1000r/min
Clock is divided into 5 parts of equivalent after shaking up, obtain mixed liquor L1, mixed liquor L2, mixed liquor L3, mixed liquor L4, mixed liquor L5；
(13) mixed liquor L1 and 2.6 grams of etamon chlorides are added in mixed liquor K1, stir 3 under the conditions of 1000r/min
Minute, obtain mixed liquor M1；
(14) substance C is added in mixed liquor M1, is shaken 35 minutes in the shaking table that temperature is 40 DEG C, is filtered to remove liquid and obtains
To substance C 1, substance C 1 is placed 120 minutes in the drying box that temperature is 55 DEG C, obtains substance C 2；
(15) mixed liquor L2 and 2.9 grams of etamon chlorides are added in mixed liquor K2, stir 3 under the conditions of 1000r/min
Minute, obtain mixed liquor M2；
(16) substance C 2 is added in mixed liquor M2, is shaken 35 minutes in the shaking table that temperature is 40 DEG C, is filtered to remove liquid
Substance C 3 is obtained, substance C 3 is placed 120 minutes in the drying box that temperature is 55 DEG C, obtains substance C 4；
(17) mixed liquor L3 and 3.2 grams of etamon chlorides are added in mixed liquor K3, stir 3 under the conditions of 1000r/min
Minute, obtain mixed liquor M3；
(18) substance C 4 is added in mixed liquor M3, is shaken 35 minutes in the shaking table that temperature is 40 DEG C, is filtered to remove liquid
Substance C 5 is obtained, substance C 5 is placed 120 minutes in the drying box that temperature is 55 DEG C, obtains substance C 6；
(19) mixed liquor L4 and 3.5 grams of etamon chlorides are added in mixed liquor K4, stir 3 under the conditions of 1000r/min
Minute, obtain mixed liquor M4；
(20) substance C 6 is added in mixed liquor M4, is shaken 35 minutes in the shaking table that temperature is 40 DEG C, is filtered to remove liquid
Substance C 7 is obtained, substance C 7 is placed 120 minutes in the drying box that temperature is 55 DEG C, obtains substance C 8；
(21) mixed liquor L5 and 3.8 grams of etamon chlorides are added in mixed liquor K5, stir 3 under the conditions of 1000r/min
Minute, obtain mixed liquor M5；
(22) substance C 8 is added in mixed liquor M5, is shaken 35 minutes in the shaking table that temperature is 40 DEG C, is filtered to remove liquid
Substance C 9 is obtained, substance C 9 is placed 120 minutes in the drying box that temperature is 55 DEG C, obtains substance D；
(23) denitrifying bacterium system is added in 1000mL basic culture solution and obtains mixed liquor N, make denitrifying bacterium in mixed liquor N
Bacteria containing amount is 7.5 × 1010CFU/mL, wherein the group of basic culture solution becomes：NaNO3:2.5g/L, NaCl:7.5g/L, KH2PO4:
3.5g/L, K2HPO4:3.0g/L, FeCl3:0.15g/L, MgSO4:3.0g/L, CaCl2·2H2O:0.3g/L；
(24) substance D is cultivated to take out afterwards for 24 hours in mixed liquor N and is air-dried, the poly- ammonia of microparticle of fixed denitrifying bacterium can be obtained
Ester rubber carrier.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810766775.8A CN108821384A (en) | 2018-07-13 | 2018-07-13 | The microparticle polyurethane rubber carrier of fixed denitrifying bacterium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810766775.8A CN108821384A (en) | 2018-07-13 | 2018-07-13 | The microparticle polyurethane rubber carrier of fixed denitrifying bacterium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108821384A true CN108821384A (en) | 2018-11-16 |
Family
ID=64137172
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810766775.8A Pending CN108821384A (en) | 2018-07-13 | 2018-07-13 | The microparticle polyurethane rubber carrier of fixed denitrifying bacterium |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108821384A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103951039A (en) * | 2014-04-04 | 2014-07-30 | 北京工业大学 | Preparation and application of nitrifying bacteria immobilized bioactive filler based on polyurethane carrier |
| CN103951079A (en) * | 2014-04-04 | 2014-07-30 | 北京工业大学 | Preparation and application of denitrifying bacteria immobilized bioactive filler based on polyurethane carrier |
| CN106582534A (en) * | 2017-01-07 | 2017-04-26 | 北京源清益壤环保科技有限公司 | Modified active carbon adsorbent for removing nickel from polluted water and preparation method for modified active carbon adsorbent |
| KR20180067320A (en) * | 2016-12-12 | 2018-06-20 | (재)한국건설생활환경시험연구원 | High Durability Recycled Asphalt Concrete with Phytolith Binder And Method For Manufacturing Thereof |
| CN108217816A (en) * | 2018-03-06 | 2018-06-29 | 高姌 | For the secondary absorption reactor of hardly degraded organic substance in Adsorption waste water |
-
2018
- 2018-07-13 CN CN201810766775.8A patent/CN108821384A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103951039A (en) * | 2014-04-04 | 2014-07-30 | 北京工业大学 | Preparation and application of nitrifying bacteria immobilized bioactive filler based on polyurethane carrier |
| CN103951079A (en) * | 2014-04-04 | 2014-07-30 | 北京工业大学 | Preparation and application of denitrifying bacteria immobilized bioactive filler based on polyurethane carrier |
| KR20180067320A (en) * | 2016-12-12 | 2018-06-20 | (재)한국건설생활환경시험연구원 | High Durability Recycled Asphalt Concrete with Phytolith Binder And Method For Manufacturing Thereof |
| CN106582534A (en) * | 2017-01-07 | 2017-04-26 | 北京源清益壤环保科技有限公司 | Modified active carbon adsorbent for removing nickel from polluted water and preparation method for modified active carbon adsorbent |
| CN108217816A (en) * | 2018-03-06 | 2018-06-29 | 高姌 | For the secondary absorption reactor of hardly degraded organic substance in Adsorption waste water |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Ji et al. | New insights for enhancing the performance of constructed wetlands at low temperatures | |
| CN107488620B (en) | Cadmium-polluted rice field soil microbial passivator | |
| Tam et al. | Effect of immobilized microalgal bead concentrations on wastewater nutrient removal | |
| CN104957009A (en) | Method for regulating turfgrass heavy metal accumulation by using OTC and carbon nano-tube interlayer | |
| WO2015056185A1 (en) | Microbial consortium for nitrate and phosphate sequestration for environmental sustenance | |
| CN110304736A (en) | A kind of slow-release complex microorganism growth-promoting agent and preparation method and purposes | |
| JP2017209647A (en) | Inclusion carrier of microorganism for water treatment, water treatment method and manufacturing method of inclusion carrier | |
| CN106430553A (en) | Method for intensively inducing aerobic sludge granulation by means of humic acid | |
| CN112209778A (en) | Novel slag soil conditioner and preparation method thereof | |
| CN103409338A (en) | Microbial water quality purifying agent for aquaculture pond and preparation method and strain curing method thereof | |
| CN110373199B (en) | Composite soil repairing agent and preparation method and repairing method thereof | |
| CN111330970A (en) | Method for reducing bioavailability of heavy metal composite contaminated soil in agricultural land | |
| CN102774955A (en) | Biological purification method of landscape water | |
| CA2973961A1 (en) | Soil inoculant and method of producing same | |
| CN113414232A (en) | Method for treating high-concentration cadmium pollution through calcium-enhanced microbial mineralization and combined phytoremediation | |
| CN113200801A (en) | Soil conditioner and preparation method and application thereof | |
| CN109006283B (en) | Method for planting Dactylicapnos asiaticus | |
| CN108821384A (en) | The microparticle polyurethane rubber carrier of fixed denitrifying bacterium | |
| CN110743907A (en) | Microorganism and plant combined in-situ remediation process for petroleum-polluted soil | |
| CN105645582B (en) | A kind of quick start method of waste water treatment system short-cut nitrification and denitrification | |
| CN114134069A (en) | Pseudomonas fluorescens N1 and application thereof | |
| CN105950498A (en) | Production method of paracoccus denitrificans and biological denitrification method of paracoccus denitrificans | |
| CN108821443A (en) | For controlling the biological floating bed and its constructing technology of water body in lake blue algae growth | |
| Soetrisnanto et al. | Application of phytoremediation for herbal medicine waste and its utilization for protein production | |
| Xu et al. | The enhancement of wastewater purification efficiency in ecological floating bed aquaculture through alginate oligosaccharide treatment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20181116 |
|
| WD01 | Invention patent application deemed withdrawn after publication |