CN108796043A - Dyestuff coding method based on fluorescent marker amino acid - Google Patents
Dyestuff coding method based on fluorescent marker amino acid Download PDFInfo
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Abstract
The present invention provides a kind of dyestuff coding methods based on fluorescent marker amino acid, including sequence label；Using amino acid sequence as sequence label, along the amino acid sequence extending direction, it is divided at least two fluorescence areas, the amino acid in each fluorescence area is at least connected with a kind of fluorescent dye or quantum dot, two neighboring fluorescence area interval at least three amino acid；Greatly extend the Serial Number Range of sequence label；By using the fluorescent dye or quantum dot of different colours, the overlapping between spectrum can be effectively avoided.The present invention also provides a kind of liquid-phase chip, detection nucleic acid or albumen that can be qualitative and quantitative, while can realize high-throughput detection.
Description
Technical field
The invention belongs to genetic test fields, and in particular to the dyestuff coding method based on fluorescent marker amino acid and its use
On the way.
Background technology
Liquid chip, also known as suspension array, the core of the technology be by the method for polystyrene microsphere fluorescent staining into
Then row coding will be directed to the probe of specific nucleic acid on the microballoon of each color (or being fluorescence-encoded micro-beads) covalent cross-linking.
In application, detection sample is carried out at the same time hybridization reaction with a variety of microballoons for being connected with specific nucleic acid probe, and after the completion of reaction, instrument
Coding microball is identified respectively by two beam laser and detects the fluorescence intensity for reporting molecule on microballoon, to determine target nucleic acid
Amount detection.It is completed in the liquid phase due to entirely reacting, reaction speed is fast, and hybridization efficiency is high, and can be in a micro-reaction body
Up to up to a hundred target sequences are detected in system simultaneously.However, research in recent years the results show that multi-analyte suspension assay still have sensitivity compared with
The deficiencies of low, less reproducible, reason are mainly that the emission spectrum of different fluorescent markers is overlapped extensively in the liquid phase, are caused
A large amount of false positive results generate, and limit the further genralrlization application of multi-analyte suspension assay.Therefore, searching is a kind of can be with different face
Color distinguishes label probe, and luminous intensity is high, is not easy to be quenched, and it is to improve liquid chip that few fluorescent marker is overlapped between spectrum
The key of detection sensitivity and repeatability.
Invention content
For this purpose, distinguishing label probe and luminous intensity in different colors the technical problem to be solved in the present invention is to provide a kind of
Height, is not easy to be quenched, and dyestuff coding method and the liquid-phase chip of few fluorescent marker amino acid are overlapped between spectrum.
For this purpose, the present invention provides a kind of dyestuff coding method based on fluorescent marker amino acid, it is with amino acid sequence
The sequence label is divided at least two fluorescence areas by sequence label along the amino acid sequence extending direction, each glimmering
Amino acid in light region is at least connected with a kind of fluorescent dye or quantum dot, two neighboring fluorescence area interval at least three amino
Acid.
Preferably, the sequence label is divided at least four fluorescence areas, the length of amino acid sequence in the fluorescence area
Degree is 10-15 amino acid.
Preferably, the fluorescent dye in fluorescence area described in any two or quantum dot differ.
Preferably, only a kind of amino acid connection fluorescent dye or quantum dot in each fluorescence area, and it is same
Fluorescent dye or quantum dot in fluorescence area is identical.
Preferably, the fluorescent dye includes BODIPY, FITC, rhodamine, cumarin, xanthene, anthocyanidin, pyrene or phthalein
Cyanines；The quantum dot be selected from MgS, MgSe, MgTe, CaS, CaSe, CaTe, ZnO, ZnS, ZnSe, ZnTe, SrS, SrSe, SeTe,
CdS、CdSe、CdTe、BaS、BaSe、BaTe、HgS、HgSe、HgTe、PbSe、CaAs、InP、InAs、InCaAs、ZnS/CdS、
ZnS/CdS/ZnS、ZnS/HgS/ZnS/CdS、CdS/ZnS、CdS/Ag2S、CdS/HgS、CdS/HgS/CdS、CdS/PbS、CdS/
Cd(OH)2、CdSe/CuSe、CdSe/ZnS、CdSe/ZnSe、CdSe/CdS、CdSe/HgSe、CdSe/HgSe/CdSe、CdSe/
HgTe,CdTe/HgS,CdTe/HgTe,InAs/ZnSe,InAs/CdSe,InAs/InP,ZnS:Mn,ZnS:Cu,CdS:Mn and
CdS:Any one of Cu, and using it is above-mentioned it is any be core, silica as the core-shell type quantum point of shell；
The present invention provides a kind of labels encoded by the dyestuff coding method based on fluorescent marker amino acid
Sequence.
The present invention provides a kind of liquid-phase chips, including：
The sequence label；
The probe molecule P1 being connect with the sequence label；
Magnetic microsphere；
The probe molecule P2 being connect with magnetic microsphere, is not tied mutually between the probe molecule P1 and the probe molecule P2
It closes.
Preferably, the probe molecule P1 includes nucleotide sequence, antigen or antibody, and the probe molecule P2 includes nucleosides
Acid sequence, antigen or antibody.
Preferably, it is connected with biotin or fluorescent dye on the probe molecule P2, and is connected on probe molecule P2 glimmering
Photoinitiator dye is differed with the fluorescent dye in the sequence label.
The present invention provides a kind of methods preparing the liquid-phase chip, include the following steps：
S1. by the sequence label linking probe molecule P1；
S2. by biotin or fluorescent dye linking probe molecule P2
S3. magnetic microsphere is connected into upper probe molecule P2.
The present invention provides a kind of purposes using the liquid-phase chip in nucleic acid or Protein Detection field.
The present invention has the following advantages that compared with the prior art：
1. the dyestuff coding method provided by the invention based on fluorescent marker amino acid, using amino acid sequence as label sequence
Row, along the amino acid sequence extending direction, are divided at least two fluorescence areas, each fluorescence area by the sequence label
In amino acid be at least connected with a kind of fluorescent dye or quantum dot, two neighboring fluorescence area interval at least three amino acid so that
The fluorescence sent out between two regions does not interfere with each other.
2. the dyestuff coding method provided by the invention based on fluorescent marker amino acid, each fluorescence area connection is a kind of glimmering
Photoinitiator dye or quantum dot, carrying out permutation and combination by the fluorescent dye or quantum dot that are connected to each fluorescence area can reach pair
The purpose that sequence label is numbered.Sequence label is divided into four fluorescence areas, by 4 kinds of different fluorescent dyes or amount
Son point is combined, and 24 kinds of different combinations can be obtained, if different fluorescence areas can use identical fluorescence
Dyestuff or quantum dot, 4 fluorescence areas can obtain 256 kinds of different combinations.And so on, sequence label is got glimmering
Light region is more, can obtain more combinations, realizes the sequence label more numbered, greatly extends sequence label
Serial Number Range.In addition, by using the fluorescent dye or quantum dot of different colours, the weight between spectrum can be effectively avoided
It is folded.
3. the dyestuff coding method provided by the invention based on fluorescent marker amino acid, in each fluorescence area of sequence label
The fluorescent dye of connection or the number of quantum dot are different so that the luminous intensity of each fluorescence area is different, by each glimmering
The luminous intensity in light region, which carries out permutation and combination, can achieve the purpose that sequence label is numbered；Such as sequence label is divided
For 4 fluorescence areas, each fluorescence area can connect 10 quantum dots, and fluorescence intensity etc. is arranged in each two quantum dot
Grade, is divided into 5 fluorescence intensity grades, 4 fluorescence areas can be obtained by 54Middle different arrangement mode, and so on, label
N number of fluorescence area is arranged in sequence, and M fluorescence intensity grade is arranged in each fluorescence area, can obtain MNThe different arrangement of kind
Mode greatly extends the Serial Number Range of sequence label.
4. liquid-phase chip provided by the invention, including sequence label, the probe molecule P1 being connect with sequence label is magnetic micro-
The probe molecule P2 of ball label detects the fluorescence that the fluorescent dye of each fluorescence area of sequence label is sent out, obtains sequence label
Number, learn in sequence to be measured whether there is target nucleic acid sequence, can to target nucleic acid sequence carry out qualitative detection.
5. liquid-phase chip provided by the invention, using fluorescent dye or biotin on probe molecule P2 fluorescence intensity with
Target nucleic acid sequence concentration is directly proportional, and quantitative detection can be carried out to target nucleic acid sequence.
6. liquid-phase chip provided by the invention can realize high-throughput detection with a variety of different target sequences of one-time detection.
7. liquid-phase chip provided by the invention, probe molecule P1 includes nucleotide sequence, albumen or carbohydrate, probe molecule P2
Including nucleotide sequence, albumen or carbohydrate, realization is detected nucleotide sequence, albumen or carbohydrate.
Description of the drawings
Fig. 1 is the coding schematic diagram of the dyestuff coding method of fluorescent marker amino acid in the embodiment of the present invention；
Fig. 2 is the detects schematic diagram of liquid-phase chip in the embodiment of the present invention；
Fig. 3 is the testing result that liquid-phase chip detects ALK, APC, BRAF and EGFR gene in experimental example of the present invention.
Specific implementation mode
Illustrate embodiments of the present invention below by way of specific embodiment, unless otherwise stated, disclosed in this invention
Experimental method be all made of the art routine techniques.
Embodiment 1
The present embodiment provides a kind of dyestuff coding methods based on fluorescent marker amino acid, using amino acid sequence as label sequence
Row, length is 70 amino acid, as shown in Figure 1, sequence label is divided into four fluorescence areas, respectively fluorescence area I, fluorescence
Region II, fluorescence area III and fluorescence area IV, length are 15 amino acid, be spaced between two neighboring fluorescence area to
Few 3 amino acid, are spaced 3 amino acid between two neighboring fluorescence area in the present embodiment；In wherein fluorescence area I
Leucine connects rhodamine, and the cysteine in fluorescence area II connects anthocyanidin, and the lysine connection in fluorescence area III is fragrant
Legumin, the valine in fluorescence area IV connect phthalocyanine.
According to the above-mentioned dyestuff coding method based on fluorescent marker amino acid, to the fluorescent dye of each fluorescence area connection
Permutation and combination is carried out, each fluorescence area uses different fluorescent dyes, obtains 24 kinds of different arrangement modes, and to each row
The number as sequence label is numbered in row mode, realizes and is numbered to sequence label.
Sequence label is divided into multiple fluorescence areas by the coding method, and is spaced between two neighboring fluorescence area
At least three amino acid so that the fluorescence sent out between two regions does not interfere with each other.Each fluorescence area connects a kind of fluorescence
Dyestuff carries out permutation and combination by the fluorescent dye that connects each fluorescence area and can reach that sequence label is numbered
Purpose.For example, as shown in table 1, being illustrated to the combination of arrangement, by carrying out group to 4 kinds of different fluorescent dyes
It closes, 24 kinds of different combinations can be obtained, if different fluorescence areas can use identical fluorescent dye, 4 glimmering
Light region can obtain 256 kinds of different combinations.
Table 1
Sequence label, which is divided into 5 fluorescence areas, can obtain 625 kinds of different combinations, and so on, by label
It is more that sequence gets fluorescence area, can obtain more combinations, realizes the sequence label more numbered, greatly extends
The Serial Number Range of sequence label.In addition, by using the fluorescent dye of different colours, can effectively avoid between spectrum
Overlapping.
Embodiment 2
The present embodiment provides a kind of dyestuff coding methods based on fluorescent marker amino acid, using amino acid sequence as label sequence
Row, length are 80 amino acid, and the sequence label is divided into four fluorescence areas, respectively fluorescence area I, fluorescence area
II, fluorescence area III and fluorescence area IV, length are 15 amino acid, and at least three is spaced between two neighboring fluorescence area
Amino acid is spaced 5 amino acid between two neighboring fluorescence area in the present embodiment；Bright ammonia in wherein fluorescence area I
Acid connects pyrene, and the cysteine in fluorescence area II connects BODIPY, and the lysine in fluorescence area III connects FITC, fluorescence
Valine in the IV of region connects rhodamine.
According to the above-mentioned dyestuff coding method based on fluorescent marker amino acid, to the fluorescent dye of each fluorescence area connection
Permutation and combination is carried out, each fluorescence area uses different fluorescent dyes, obtains 24 kinds of different arrangement modes, and to each row
The number as sequence label is numbered in row mode, realizes and is numbered to sequence label.
Embodiment 3
The present embodiment provides a kind of dyestuff coding methods based on fluorescent marker amino acid, using amino acid sequence as label sequence
Row, length are 100 amino acid, and the sequence label is divided into five fluorescence areas, respectively fluorescence area I, fluorescence area
II, fluorescence area III, fluorescence area IV and fluorescence area V, length are 15 amino acid, between two neighboring fluorescence area
It is spaced at least three amino acid, is spaced 5 amino acid between two neighboring fluorescence area in the present embodiment；Wherein phosphor region
Leucine in the I of domain connects xanthene, and the cysteine in fluorescence area II connects anthocyanidin, the lysine in fluorescence area III
Pyrene is connected, the valine in fluorescence area IV connects BODIPY, and the serine in fluorescence area V connects rhodamine.
According to the above-mentioned dyestuff coding method based on fluorescent marker amino acid, above-mentioned 5 kinds of fluorescent dyes (xanthene, flower are selected
Green element, pyrene, BODIPY and rhodamine) above-mentioned 5 fluorescence areas are marked in permutation and combination, realize 625 sequence labels volumes
Number.
Embodiment 4
The present embodiment provides a kind of dyestuff coding methods based on fluorescent marker amino acid, using amino acid sequence as label sequence
Row, length are 30 amino acid, and the sequence label is divided into 2 fluorescence areas, respectively fluorescence area I, fluorescence area II,
Length is 10 amino acid, is spaced at least three amino acid between two neighboring fluorescence area, is in the present embodiment adjacent two
10 amino acid are spaced between a fluorescence area；Cysteine in wherein fluorescence area I connects phthalocyanine, in fluorescence area II
Serine connects anthocyanidin.
According to the above-mentioned dyestuff coding method based on fluorescent marker amino acid, to the fluorescent dye of each fluorescence area connection
Permutation and combination is carried out, each fluorescence area uses different fluorescent dyes, obtains 2 kinds of different arrangement modes, and to each row
The number as sequence label is numbered in row mode, realizes and is numbered to sequence label.
As interchangeable embodiment, sequence label can be divided into two, three, four, five or more fluorescence areas,
As long as being spaced at least three amino acid between two neighboring fluorescence area, only a kind of amino acid connects one in each fluorescence area
Kind fluorescent dye.
As interchangeable embodiment, fluorescent dye can be BODIPY, FITC, rhodamine, cumarin, xanthene, flower
Green element, pyrene and phthalocyanine.
Embodiment 5
The present embodiment provides a kind of dyestuff coding methods based on fluorescent marker amino acid, using amino acid sequence as label sequence
Row, length are 95 amino acid, and the sequence label is divided into four fluorescence areas, respectively fluorescence area I, fluorescence area
II, fluorescence area III and fluorescence area IV, length are 15 amino acid, and at least three is spaced between two neighboring fluorescence area
Amino acid is spaced 3 amino acid between two neighboring fluorescence area in the present embodiment；Half Guang in wherein fluorescence area I
Propylhomoserin connects CdTe, and the valine in fluorescence area II connects CaS, and the serine in fluorescence area III connects CdS, phosphor region
Leucine in the IV of domain connects CdSe.
Sequence label is divided into multiple fluorescence areas by the coding method, and is spaced between two neighboring fluorescence area
At least three amino acid so that the fluorescence sent out between two regions does not interfere with each other.Each fluorescence area connects a kind of quantum
Point, the mesh that sequence label is numbered can be reached by carrying out permutation and combination by the quantum dot connected to each fluorescence area
's.For example, as shown in table 2, illustrated to the combination of arrangement, it, can by being combined to 4 kinds of different quantum dots
To obtain 24 kinds of different combinations, if different fluorescence areas can use identical quantum dot, 4 fluorescence areas can
To obtain 256 kinds of different combinations.
Table 2
Sequence label, which is divided into 5 fluorescence areas, can obtain 625 kinds of different combinations, and so on, by label
It is more that sequence gets fluorescence area, can obtain more combinations, realizes the sequence label more numbered, greatly extends
The Serial Number Range of sequence label.In addition, by using the quantum dot of different colours, the weight between spectrum can be effectively avoided
It is folded.
Embodiment 6
The present embodiment provides a kind of dyestuff coding methods based on fluorescent marker amino acid, using amino acid sequence as label sequence
Row, length are 95 amino acid, and the sequence label is divided into four fluorescence areas, respectively fluorescence area I, fluorescence area
II, fluorescence area III and fluorescence area IV, length are 15 amino acid, and at least three is spaced between two neighboring fluorescence area
Amino acid is spaced 5 amino acid between two neighboring fluorescence area in the present embodiment；Amino in wherein fluorescence area I
Amino acid in acid connection CdTe, fluorescence area II connects CaS, and the amino acid in fluorescence area III connects CdS, fluorescence area
Amino acid in IV connects CdSe.It is a fluorescence intensity grade often to connect two quantum dots in each fluorescence area, such as glimmering
There are two amino acid to connect CdTe in the I of light region, then the fluorescence intensity grade of fluorescence area I is CdTe intensity I, fluorescence area II
In there are four amino acid connect CaS, then the fluorescence intensity grade of fluorescence area II be CaS intensity II, fluorescence area III in have six
A amino acid connects CdS, then the fluorescence intensity grade of fluorescence area III is to have eight ammonia in CdS intensity III, fluorescence area IV
Base acid connects CdSe, then the fluorescence intensity grade of fluorescence area IV is CdSe intensity IV.Each fluorescence area point in the present embodiment
For 5 fluorescence intensity grades：Intensity I, intensity II, intensity III, intensity IV and intensity V pass through what is connected to each fluorescence area
The fluorescence intensity grade of quantum dot, which carries out permutation and combination, can achieve the purpose that sequence label is numbered.For example, such as 3 institute of table
Show, is illustrated to the combination of arrangement, it, can be with by being combined to 4 kinds of different quantum dot fluorescence strength grades
Obtain 54The different combination of kind, that is, have 54A number.
Table 3
Embodiment 7
The present embodiment provides a kind of dyestuff coding methods based on fluorescent marker amino acid, using amino acid sequence as label sequence
Row, length are 125 amino acid, and the sequence label is divided into five fluorescence areas, respectively fluorescence area I, fluorescence area
II, fluorescence area III, fluorescence area IV and fluorescence area V, length are 15 amino acid, between two neighboring fluorescence area
It is spaced at least three amino acid, is spaced 5 amino acid between two neighboring fluorescence area in the present embodiment；Wherein phosphor region
Amino acid in the I of domain connects CdTe, and the amino acid in fluorescence area II connects CaS, the amino acid connection in fluorescence area III
Amino acid in CdS, fluorescence area IV connects CdSe, and the amino acid in fluorescence area V connects HgS.It is every in each fluorescence area
Two quantum dots of connection are a fluorescence intensity grade, such as there are two amino acid to connect CdTe in fluorescence area I, then phosphor region
The fluorescence intensity grade of domain I is that there are four amino acid to connect CaS in CdTe intensity I, fluorescence area II, then fluorescence area II's is glimmering
Light intensity level is that there are six amino acid to connect CdS in CaS intensity II, fluorescence area III, then the fluorescence of fluorescence area III is strong
Degree grade is to have eight amino acid to connect CdSe in CdS intensity III, fluorescence area IV, then the fluorescence intensity etc. of fluorescence area IV
Grade is CdSe intensity IV；There are ten amino acid connection HgS in fluorescence area V, then the fluorescence intensity grade of fluorescence area V is HgS
Intensity V, each fluorescence area is divided into 6 fluorescence intensity grades in the present embodiment：Intensity I, intensity II, intensity III, intensity IV,
Intensity V and intensity VI, carrying out permutation and combination to the fluorescence intensity grade of the quantum dot of each fluorescence area connection can reach pair
The purpose that sequence label is numbered, by the way that 5 kinds of different quantum dots, 6 fluorescence intensity grades are combined, can obtain
65The different combination of kind, that is, have 65A number.
As interchangeable embodiment, sequence label can be divided into two, three, four, five or more fluorescence areas,
As long as being spaced at least 5bp between two neighboring fluorescence area, amino acid connects a kind of quantum dot in each fluorescence area.
As interchangeable embodiment, quantum dot can be MgS, MgSe, MgTe, CaS, CaSe, CaTe, ZnO, ZnS,
ZnSe、ZnTe、SrS、SrSe、SeTe、CdS、CdSe、CdTe、BaS、BaSe、BaTe、HgS、HgSe、HgTe、PbSe、CaAs、
InP、InAs、InCaAs、ZnS/CdS、ZnS/CdS/ZnS、ZnS/HgS/ZnS/CdS、CdS/ZnS、CdS/Ag2S、CdS/HgS、
CdS/HgS/CdS、CdS/PbS、CdS/Cd(OH)2、CdSe/CuSe、CdSe/ZnS、CdSe/ZnSe、CdSe/CdS、CdSe/
HgSe、CdSe/HgSe/CdSe、CdSe/HgTe、CdTe/HgS、CdTe/HgTe、InAs/ZnSe、InAs/CdSe、InAs/
InP,ZnS:Mn,ZnS:Cu,CdS:Mn and CdS:Any one of Cu, and using it is above-mentioned it is any be core, silica as shell
Core-shell type quantum point.
Sequence label is divided into N number of fluorescence area, is spaced at least three amino acid between two neighboring fluorescence area, each
Amino acid connects a kind of quantum dot in fluorescence area, and each fluorescence area is divided into M fluorescence intensity grade, by different to N kinds
Quantum dot, M fluorescence intensity grade be combined, and can obtain MNThe different combination of kind, that is, have MNA number, and it is right
The number as sequence label is numbered in each arrangement mode, realizes and is numbered to sequence label.
Embodiment 8
A kind of liquid-phase chip provided in this embodiment includes the dyestuff based on fluorescent marker amino acid by embodiment 1
The sequence label that coding method encodes；
One section of probe molecule P1 and one section of probe molecule P2, probe molecule P1 energy and target nucleic acid are designed according to target nucleic acid sequence
The hybridization of the end of sequence 5 ' combines, and probe molecule P2 can be held with target nucleic acid sequence 3 ' hybridizes combination, probe molecule P1 and probe molecule P2
It is not complementary, fluorescent dye FITC is connected on probe molecule P2.As shown in Fig. 2, probe molecule P1 is connected on sequence label,
Probe molecule P2 is connected on magnetic microsphere；By the sequence label of linking probe molecule P1 with linking probe molecule P2's
The magnetic microsphere mixing.
Sample to be tested is subjected to PCR amplification using universal primer, then pcr amplification product is added to the liquid-phase chip
In, pcr amplification product carries out hybridization reaction with sequence label and magnetic microsphere simultaneously, if sample to be tested contains target nucleic acid sequence
Row, then the probe molecule P2 on the probe molecule P1 and magnetic microsphere on sequence label can be mutual by base with target nucleic acid sequence
Raw specific binding is reissued, the compound of gained can be isolated by Magnetic Isolation from reaction system using magnetic microsphere
Come, it is directly proportional to target nucleic acid sequence concentration further according to the fluorescence intensity of the fluorescent dye on probe molecule P2, to target nucleic acid sequence
Carry out quantitative detection.If not containing target nucleic acid sequence in sample to be tested, can not lead to for the probe molecule P1 of the target nucleic acid
It crosses target nucleic acid sequence and is combined with the probe molecule P2 that magnetic microsphere marks, do not have magnetism, to be taken out in Magnetic Isolation.
Embodiment 9
Include being compiled by the dyestuff based on fluorescent marker amino acid of embodiment 6 the present embodiment provides a kind of liquid-phase chip
The sequence label that code method encodes, i.e. sequence label are amino acid sequence, and length is 95 amino acid, by sequence label point
At four fluorescence areas, respectively fluorescence area I, fluorescence area II, fluorescence area III and fluorescence area IV, length are 15
A amino acid is spaced at least three amino acid between two neighboring fluorescence area；CdTe, CaS, CdS and CdSe are connected respectively to
On a kind of amino acid in four fluorescence areas, the leucine in wherein fluorescence area I connects CdTe, the silk in fluorescence area II
Propylhomoserin connects CaS, and the valine in fluorescence area III connects CdS, and the cysteine in fluorescence area IV connects CdSe, each
It is a fluorescence intensity grade that two quantum dots are often connected in fluorescence area, such as there are two amino acid to connect in fluorescence area I
CdTe, then the fluorescence intensity grade of fluorescence area I is that there are four amino acid to connect CaS in CdTe intensity I, fluorescence area II, then
The fluorescence intensity grade of fluorescence area II is that there are six amino acid to connect CdS in CaS intensity II, fluorescence area III, then phosphor region
The fluorescence intensity grade of domain III is to have eight amino acid to connect CdSe in CdS intensity III, fluorescence area IV, then fluorescence area IV
Fluorescence intensity grade be CdSe intensity IV.Each fluorescence area is divided into 5 fluorescence intensity grades in the present embodiment：Intensity I, by force
II, intensity III, intensity IV and intensity V are spent, arrangement group is carried out to the fluorescence intensity grade of the quantum dot of each fluorescence area connection
Conjunction can achieve the purpose that sequence label is numbered.By being combined to 4 kinds of different quantum dot fluorescence strength grades,
625 kinds of different combinations can be obtained, and the number as sequence label is numbered to each arrangement mode.
Different probe molecule P1 and probe molecule P2, probe molecule P1 are designed according to 625 kinds of different target nucleic acid sequences
Can be held with target nucleic acid sequence 5 ' and hybridize combinations, probe molecule P2 can hybridize combination with the end of target nucleic acid sequence 3 ', probe molecule P1 with
Probe molecule P2 is not complementary, and fluorescent dye FITC is connected on probe molecule P2.Probe molecule P1 is connected on sequence label,
Probe molecule P2 is connected on magnetic microsphere；By the sequence label of linking probe molecule P1 with linking probe molecule P2's
The magnetic microsphere mixing.
Sample to be tested is subjected to PCR amplification using universal primer, then pcr amplification product is added to the liquid-phase chip
In, the pcr amplification product carries out hybridization reaction with sequence label and magnetic microsphere simultaneously, if sample to be tested contains target nucleic acid
Sequence, then the probe molecule P2 on the probe molecule P1 and magnetic microsphere on sequence label can pass through base with target nucleic acid sequence
Complementation is specifically bound, and the compound of gained can be isolated by Magnetic Isolation from reaction system using magnetic microsphere
Come, detects the fluorescence that the fluorescent dye of each fluorescence area of sequence label is sent out, obtain the number of sequence label, learn and wait being sequenced
It whether there is target nucleic acid sequence in row, qualitative detection can be carried out to target nucleic acid sequence；Recycle the fluorescence on probe molecule P2
The fluorescence intensity of dyestuff FITC is directly proportional to target nucleic acid sequence concentration, and quantitative detection is carried out to target nucleic acid sequence.
Experimental example 1
This experimental example uses the liquid-phase chip in embodiment 9 to detect 4 relevant genes of tumor-targeting drug medication, including
ALK, APC, BRAF and EGFR.Sequence label as shown in SEQ NO.1, the probe molecule P1 of ALK gene as shown in SEQ NO.2,
Probe molecule P2 is as shown in SEQ NO.3, and the probe molecule P1 of apc gene is as shown in SEQ NO.4, probe molecule P2 such as SEQ
Shown in NO.5；The probe molecule P1 of BRAF gene is as shown in SEQ NO.6, and probe molecule P2 is as shown in SEQ NO.7；EGFR gene
Probe molecule P1 as shown in SEQ NO.8, probe molecule P2 is as shown in SEQ NO.9.
Fig. 3 is mixed using the relevant gene of flow cytomery tumor-targeting drug medication using liquid-phase chip of the present invention
The standard curve for closing sample, the regression equation of each standard curve is acquired with least squares method, and R2 is above 0.99, shows that fluorescence contaminates
Expect that FITC fluorescence intensities are directly proportional to target nucleic acid sequence concentration, can quantitative detection be carried out to target nucleic acid sequence accordingly.
The above embodiments are merely examples for clarifying the description, and does not limit the embodiments.For institute
For the those of ordinary skill in category field, other various forms of variations or change can also be made on the basis of the above description
Dynamic, obvious changes or variations extended from this are still within the protection scope of the invention.
Sequence table
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Claims (11)
1. a kind of dyestuff coding method based on fluorescent marker amino acid, which is characterized in that
Using amino acid sequence as sequence label, along the amino acid sequence extending direction, the sequence label is divided at least
Two fluorescence areas, the amino acid in each fluorescence area are at least connected with a kind of fluorescent dye or quantum dot, two neighboring fluorescence
It is interregional every at least three amino acid.
2. the dyestuff coding method based on fluorescent marker amino acid according to claim 1, which is characterized in that the label sequence
It arranges and is divided at least four fluorescence areas, the length of amino acid sequence is 10-15 amino acid in the fluorescence area.
3. the dyestuff coding method according to claim 1 or claim 2 based on fluorescent marker amino acid, which is characterized in that arbitrary two
Fluorescent dye or quantum dot in a fluorescence area differ.
4. according to the dyestuff coding method based on fluorescent marker amino acid described in claim any one of 1-3, which is characterized in that every
Only a kind of amino acid connection fluorescent dye or quantum dot in a fluorescence area, and the fluorescence dye in the same fluorescence area
Material or quantum dot are identical.
5. according to any dyestuff coding methods based on fluorescent marker amino acid of claim 1-4, which is characterized in that described
Fluorescent dye includes BODIPY, FITC, rhodamine, cumarin, xanthene, anthocyanidin, pyrene or phthalocyanine；The quantum dot be selected from MgS,
MgSe、MgTe、CaS、CaSe、CaTe、ZnO、ZnS、ZnSe、ZnTe、SrS、SrSe、SeTe、CdS、CdSe、CdTe、BaS、
BaSe、BaTe、HgS、HgSe、HgTe、PbSe、CaAs、InP、InAs、InCaAs、ZnS/CdS、ZnS/CdS/ZnS、ZnS/
HgS/ZnS/CdS、CdS/ZnS、CdS/Ag2S、CdS/HgS、CdS/HgS/CdS、CdS/PbS、CdS/Cd(OH)2、CdSe/
CuSe、CdSe/ZnS、CdSe/ZnSe、CdSe/CdS、CdSe/HgSe、CdSe/HgSe/CdSe、CdSe/HgTe、CdTe/HgS、
CdTe/HgTe,InAs/ZnSe,InAs/CdSe,InAs/InP,ZnS:Mn,ZnS:Cu,CdS:Mn and CdS:Any in Cu
Kind, and using it is above-mentioned it is any be core, silica as the core-shell type quantum point of shell.
6. the label sequence that the dyestuff coding method based on fluorescent marker amino acid encodes described in claim any one of 1-5
Row.
7. a kind of liquid-phase chip, which is characterized in that including：
Sequence label described in claim 6；
The probe molecule P1 being connect with the sequence label；
Magnetic microsphere；
The probe molecule P2 being connect with magnetic microsphere, is not combined with each other between the probe molecule P1 and the probe molecule P2.
8. liquid-phase chip according to claim 7, which is characterized in that the probe molecule P1 includes nucleotide sequence, resists
Former or antibody, the probe molecule P2 include nucleotide sequence, antigen or antibody.
9. liquid-phase chip according to claim 8, which is characterized in that be connected with biotin or glimmering on the probe molecule P2
Photoinitiator dye, and the fluorescent dye connected on probe molecule P2 is differed with the fluorescent dye in the sequence label.
10. a kind of method preparing the liquid-phase chip described in claim 7-9, which is characterized in that include the following steps：
S1. by the sequence label linking probe molecule P1 described in claim 6；
S2. by biotin or fluorescent dye linking probe molecule P2
S3. magnetic microsphere is connected into upper probe molecule P2.
11. a kind of liquid-phase chip using described in claim 7-9 is in the purposes in nucleic acid or Protein Detection field.
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| CN201810600979.4A CN108796043B (en) | 2018-06-12 | 2018-06-12 | Dye coding method based on fluorescence labeling amino acid |
| PCT/CN2018/091983 WO2019237406A1 (en) | 2018-06-12 | 2018-06-20 | Dye encoding method based on fluorescently-labeled amino acid |
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