CN108795794B - Bacillus pumilus, screening method thereof, preparation method of microbial inoculum and application of microbial inoculum - Google Patents
Bacillus pumilus, screening method thereof, preparation method of microbial inoculum and application of microbial inoculum Download PDFInfo
- Publication number
- CN108795794B CN108795794B CN201710821762.1A CN201710821762A CN108795794B CN 108795794 B CN108795794 B CN 108795794B CN 201710821762 A CN201710821762 A CN 201710821762A CN 108795794 B CN108795794 B CN 108795794B
- Authority
- CN
- China
- Prior art keywords
- bacillus pumilus
- microbial inoculum
- culture
- formulation
- potassium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 241000194103 Bacillus pumilus Species 0.000 title claims abstract description 56
- 239000002068 microbial inoculum Substances 0.000 title claims description 18
- 238000000034 method Methods 0.000 title claims description 14
- 238000012216 screening Methods 0.000 title description 11
- 238000002360 preparation method Methods 0.000 title description 5
- 108020004465 16S ribosomal RNA Proteins 0.000 claims abstract description 7
- 238000004321 preservation Methods 0.000 claims abstract description 6
- 239000001963 growth medium Substances 0.000 claims description 11
- 238000012258 culturing Methods 0.000 claims description 9
- 230000008635 plant growth Effects 0.000 claims description 6
- 230000001737 promoting effect Effects 0.000 claims description 6
- 230000003213 activating effect Effects 0.000 claims description 2
- 230000004913 activation Effects 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 238000007865 diluting Methods 0.000 claims description 2
- 238000000855 fermentation Methods 0.000 claims description 2
- 230000004151 fermentation Effects 0.000 claims description 2
- 238000011218 seed culture Methods 0.000 claims description 2
- 238000009472 formulation Methods 0.000 claims 5
- 239000000203 mixture Substances 0.000 claims 5
- 230000000694 effects Effects 0.000 abstract description 9
- 244000005700 microbiome Species 0.000 abstract description 6
- 244000144972 livestock Species 0.000 abstract description 3
- 239000002689 soil Substances 0.000 description 17
- 241000208125 Nicotiana Species 0.000 description 13
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 13
- 241000196324 Embryophyta Species 0.000 description 11
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 8
- 239000011591 potassium Substances 0.000 description 8
- 229910052700 potassium Inorganic materials 0.000 description 8
- 230000012010 growth Effects 0.000 description 7
- 238000002474 experimental method Methods 0.000 description 6
- 238000011160 research Methods 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 4
- 244000221633 Brassica rapa subsp chinensis Species 0.000 description 4
- 230000035558 fertility Effects 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 230000000877 morphologic effect Effects 0.000 description 3
- 239000003181 biological factor Substances 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 238000009629 microbiological culture Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 244000000000 soil microbiome Species 0.000 description 2
- KEQXNNJHMWSZHK-UHFFFAOYSA-L 1,3,2,4$l^{2}-dioxathiaplumbetane 2,2-dioxide Chemical group [Pb+2].[O-]S([O-])(=O)=O KEQXNNJHMWSZHK-UHFFFAOYSA-L 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 108020000946 Bacterial DNA Proteins 0.000 description 1
- 240000007124 Brassica oleracea Species 0.000 description 1
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 1
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 1
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 1
- 108091026890 Coding region Proteins 0.000 description 1
- 108020004414 DNA Proteins 0.000 description 1
- 241000192125 Firmicutes Species 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- DLHONNLASJQAHX-UHFFFAOYSA-N aluminum;potassium;oxygen(2-);silicon(4+) Chemical compound [O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[Al+3].[Si+4].[Si+4].[Si+4].[K+] DLHONNLASJQAHX-UHFFFAOYSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000721 bacterilogical effect Effects 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000003113 dilution method Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000006799 invasive growth in response to glucose limitation Effects 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000004382 potting Methods 0.000 description 1
- 238000012257 pre-denaturation Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention belongs to the technical field of microorganisms, and particularly relates to bacillus pumilus and application thereof, wherein the preservation number of the strain is CGMCC No.13448, the bacillus pumilus is named as bacillus pumilus BP16, and the 16S rDNA sequence of the bacillus pumilus is shown as SEQ ID No: 1, the bacillus pumilus has a good potassium-dissolving effect, is safe to people, livestock and crops, and is environment-friendly.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and particularly relates to bacillus pumilus, a screening method thereof, and a preparation method and application of a microbial inoculum.
Background
Soil microorganisms and plant roots form a stable dynamic system, in which they interact and influence each other, the plant roots provide nutrients for the soil microorganisms, and the soil microorganisms can improve soil fertility and promote plant growth, so that the soil, the plants and the microorganisms interact and are closely related in agricultural production. Potassium is a large amount of nutrient elements necessary for the growth and development of crops, and the research on the effectiveness of the potassium in soil has extremely important significance for improving the soil fertility. The screening and research of potassium-decomposing bacteria in different soils are of great significance for developing microbial strain resources, improving soil fertility, improving the quality of agricultural products and improving ecological environment.
Most of the existing researches are focused on the research of functional flora of a single-function biological factor or different biological factors with the same function, and the screening research of multifunctional soil bacteria strains is little.
Disclosure of Invention
The bacillus pumilus provided by the invention has a good potassium-dissolving effect, is safe to people, livestock and crops, and is environment-friendly. The technical scheme is as follows:
the Bacillus pumilus provided by the invention has been preserved in the China general microbiological culture Collection center (address: No. 3 of Xilu No.1 of Beijing Kogyo-Yang district, Beijing) within 12-14 months in 2016, and the preservation registration number is CGMCC No. 13448. The Bacillus pumilus (Bacillus pumilus) is named as Bacillus pumilus BP 16.
The bacteriological characteristics of the Bacillus pumilus are as follows: gram positive bacteria, rod-shaped, rounded end. The bacterial colony is milk white in color, sawtoothed in periphery, flat, without raised, concentric ring and no luster.
The sequence table of the bacillus pumilus 16S rDNA is shown in a sequence table SEQ ID NO.1, and the bacillus pumilus 16S rDNA is compared with a sequence reported on genbank to finally determine that the strain is the bacillus pumilus.
The screening method of the bacillus pumilus comprises the following steps: (1) dissolving 10g tobacco field soil sample in 100ml sterile water, shaking for 20min, preparing soil suspension by 10-fold dilution method, selecting 10-3And 10-5Coating two gradients in an NA plate, setting 4 gradients for each gradient repeatedly, culturing in a constant-temperature incubator at 28 ℃ for 72h, selecting a single colony, and performing streak culture in the NA plate to obtain bacillus; (2) inoculating the obtained bacteria into a potassium-dissolving primary screening culture medium, culturing at 28 ℃ for 120h to obtain a strain with potassium-dissolving activity, and transferring to an LB (Langerhans-Blodgett) plate for culture and preservation.
A Bacillus pumilus bacterial agent is prepared from Bacillus pumilus through activating, culturing seeds, and fermenting. Among them, preferably, the activation method is to inoculate the Bacillus pumilus in LB culture medium and culture at 28 ℃ for 24 h. The seed culture method comprises the steps of inoculating the activated bacillus pumilus into a culture medium, and carrying out shaking culture at 28 ℃ and 150r/min for 12 h. The fermentation method comprises the steps of inoculating the cultured bacillus pumilus into a culture medium according to the volume ratio of 1%, carrying out shaking culture at the temperature of 28 ℃ and at the speed of 150r/min for 72 hours, and diluting the obtained microbial inoculum by 100 times for use.
The bacillus pumilus can be applied to potassium dissolution and plant growth promotion, and the prepared bacillus pumilus microbial inoculum can promote plant growth.
Compared with the prior art, the invention has the following advantages:
(1) the bacillus pumilus is a tobacco field soil bacterium, is safe to people, livestock and crops, and is environment-friendly;
(2) the bacillus pumilus has a good potassium dissolving effect, wherein the potassium dissolving effect refers to the effect of converting potassium which cannot be utilized by plants (such as potassium formed by minerals in potassium feldspar) into soluble potassium which can be utilized by the plants, has a very important significance for improving the soil fertility, and has an effect of promoting the growth of the plants, wherein the plants are cabbages or tobaccos. The method for promoting the plant growth is characterized by promoting at least one of the indexes of leaf length, leaf width, leaf number, stem thickness and total root length to increase, and has great application value for plant cultivation, especially for the cultivation of economic plants.
Drawings
FIG. 1 shows the colony morphology of Bacillus pumilus BP16 grown on LB medium;
FIG. 2 is a gram-stained microscope (magnification:. times.1,000) of Bacillus pumilus BP 16;
FIG. 3 is a phylogenetic tree diagram of Bacillus pumilus BP 16.
Detailed Description
First, acquisition of Bacillus pumilus
Tobacco field soil was collected from the cities of the provinces of Shandong and Changle county, and 31 bacteria were co-isolated and purified by soil dilution for further experiments.
Second, screening of bacterial strains
Inoculating the bacteria separated and purified from the tobacco field soil into a potassium-dissolving primary screening culture medium. After culturing at 28 ℃ for 120 hours, colonies with a transparent circle were observed and recorded, and the diameter (D) of the transparent circle and the diameter (D) of the colony were measured.
The strain Zcg obtained by the potassium-dissolving primary screening plate method has good inorganic phosphorus-dissolving and potassium-dissolving effects.
Table 1: statistical table of potassium-dissolving effect of strain Zcg
Identification of Strain Zcg
1. Morphological characterization of Strain Zcg
The morphological picture of the single colony after 48h of culture on LB solid medium at 28 ℃ is shown in figure 1, and the nearly circular colony which is milky opaque, saw-toothed in periphery, flat in surface, free of bulge, free of concentric circles and free of light is formed. A small number of strains were picked and gram stained, and the staining results were observed under a microscope, as shown in FIG. 2.
2. Molecular characterization of Strain Zcg
The 16SrDNA fragment is amplified by using the universal primers 5'-AGAGTTTGATCCTGGCTCAG-3' and 5'-CGGTGTGTACAAGGCCC-3' by using the bacterial DNA as a template for sequencing analysis. The reaction is carried out in a system: pre-denaturation at 95 ℃ for 5 min; 30 cycles (94 ℃ 40S, 56 ℃ 40S, 72 ℃ 90S); extension at 72 ℃ for 10 min. The universal primers were sequenced and the resulting 16S rDNA gene sequence was analyzed for homology in GenBank using the BLAST search program. Meanwhile, according to a sequence with higher similarity to the 16S rDNA sequence of the strain in GenBank, a phylogenetic tree is constructed by MEGA4.0 software, and the phylogenetic tree of the strain is shown in figure 3.
The coding sequence of 16S rDNA of the strain Zcg is shown in SEQ ID No: 1 is shown.
Fourth, preservation of the Strain
Based on the morphological identification and molecular identification results in the third step, the strain Zcg belongs to Bacillus pumilus (Bacillus pumilus), is preserved in the China general microbiological culture Collection center (CGMCC) at 2016, 12, 14 and has the preservation number of CGMCC No.13448 and is named as Bacillus pumilus BP 16.
Fifthly, preparation of microbial inoculum
A single colony of Bacillus pumilus BP16 is selected and inoculated in an LB culture medium for shaking culture at 28 ℃ and 150r/min for 24h, the activated Bacillus pumilus is inoculated in the culture medium for shaking culture at 28 ℃ and 150r/min for 12h, the activated Bacillus pumilus is inoculated in the culture medium at the ratio of 1 percent for shaking culture at 28 ℃ and 150r/min for 72h, and the obtained microbial inoculum can be diluted by 100 times for use.
The bacillus pumilus BP16 microbial inoculum is obtained according to the steps.
Application of bacillus pumilus microbial inoculum in promoting plant growth
1. Growth promotion experiment of pakchoi
5d, transplanting the pakchoi cultured by a potting method into a new seedling culture tray for 3d, and respectively sucking 15mL and 100mL of the microbial inoculum diluted by 100 times and irrigating the diluted microbial inoculum into the rhizosphere soil of the pakchoi seedlings.
And (5) measuring the leaf width, the leaf length and the plant height of the pakchoi after culturing for 20 days in a room temperature environment.
Table 2: small white growth promoting experiment measurement statistical table
2. Tobacco growth promotion experiment
Culturing tobacco by a pot culture method for 30d, transplanting the tobacco into a new seedling culture plate for 3d, and then sucking 100mL of the diluted microbial inoculum and irrigating the microbial inoculum in the rhizosphere soil of the tobacco seedlings.
And (3) measuring the leaf width, leaf length, leaf number, plant height, stem thickness, root length, total root area and root diameter of the tobacco after culturing for 30 days in a room temperature environment.
Table 3: statistical chart for aerial part measurement of tobacco growth promotion experiment
Table 4: tobacco growth promotion experiment underground part measurement statistical chart
Various other modifications and changes may be made by those skilled in the art based on the above-described technical solutions and concepts, and all such modifications and changes should fall within the scope of the claims of the present invention.
Sequence listing
<110> Weifang market tobacco monopoly bureau
<120> Bacillus pumilus, screening method thereof, preparation method of microbial inoculum and application
<130> 1
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1443
<212> DNA
<213> Bacillus pumilus (Bacillus pumilus)
<400> 1
gaatctgcac ttcggcggct ggctcataaa ggttacctca ccgacttcgg gtgttgcaaa 60
ctctcgtggt gtgacgggcg gtgtgtacaa ggcccgggaa cgtattcacc gcggcatgct 120
gatccgcgat tactagcgat tccagcttca cgcagtcgag ttgcagactg cgatccgaac 180
tgagaacaga tttgtgggat tggctaaacc ttgcggtctc gcagcccttt gttctgtcca 240
ttgtagcacg tgtgtagccc aggtcataag gggcatgatg atttgacgtc atccccacct 300
tcctccggtt tgtcaccggc agtcacctta gagtgcccaa ctgaatgctg gcaactaaga 360
tcaagggttg cgctcgttgc gggacttaac ccaacatctc acgacacgag ctgacgacaa 420
ccatgcacca cctgtcactc tgtccccgaa gggaaagccc tatctctagg gttgtcagag 480
gatgtcaaga cctggtaagg ttcttcgcgt tgcttcgaat taaaccacat gctccaccgc 540
ttgtgcgggc ccccgtcaat tcctttgagt ttcagtcttg cgaccgtact ccccaggcgg 600
agtgcttaat gcgttagctg cagcactaag gggcggaaac cccctaacac ttagcactca 660
tcgtttacgg cgtggactac cagggtatct aatcctgttc gctccccacg ctttcgctcc 720
tcagcgtcag ttacagacca gagagtcgcc ttcgccactg gtgttcctcc acatctctac 780
gcatttcacc gctacacgtg gaattccact ctcctcttct gcactcaagt ttcccagttt 840
ccaatgaccc tccccggttg agccgggggc tttcacatca gacttaagaa accgcctgcg 900
agccctttac gcccaataat tccggacaac gcttgccacc tacgtattac cgcggctgct 960
ggcacgtagt tagccgtggc tttctggtta ggtaccgtca aggtgcaagc agttactctt 1020
gcacttgttc ttccctaaca acagagcttt acgatccgaa aaccttcatc actcacgcgg 1080
cgttgctccg tcagactttc gtccattgcg gaagattccc tactgctgcc tcccgtagga 1140
gtctgggccg tgtctcagtc ccagtgtggc cgatcaccct ctcaggtcgg ctacgcatcg 1200
tcgccttggt gagccgttac ctcaccaact agctaatgcg ccgcgggtcc atctgtaagt 1260
gacagccgaa accgtctttc atccttgaac catgcggttc aaggaactat ccggtattag 1320
ctccggtttc ccggagttat cccagtctta caggcaggtt acccacgtgt tactcacccg 1380
tccgccgcta acatccggga gcaagctccc ttctgtccgc tcgactgcat tatagaccgc 1440
agg 1443
Claims (6)
1. A bacillus pumilus agent is characterized in that: the microbial inoculum is obtained by activating, seed culturing and fermenting the bacillus pumilus, the preservation number of the bacillus pumilus is CGMCC No.13448, and the bacillus pumilus is named as bacillus pumilus BP 16.
2. The Bacillus pumilus formulation of claim 1, wherein: the 16S rDNA sequence of the bacillus pumilus is shown as SEQ ID No: 1 is shown.
3. The Bacillus pumilus formulation of claim 1, wherein: the activation method comprises the steps of inoculating the bacillus pumilus into an LB culture medium and culturing for 24 hours at 28 ℃.
4. The Bacillus pumilus formulation of claim 1, wherein: the seed culture method comprises the steps of inoculating the activated bacillus pumilus into a culture medium, and carrying out shaking culture at 28 ℃ and 150r/min for 12 h.
5. The Bacillus pumilus formulation of claim 1, wherein: the fermentation method comprises the steps of inoculating the cultured bacillus pumilus into a culture medium according to the volume ratio of 1%, carrying out shaking culture at the temperature of 28 ℃ and at the speed of 150r/min for 72 hours, and diluting the obtained microbial inoculum by 100 times for use.
6. The use of the bacillus pumilus formulation of claim 1 for promoting plant growth.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710821762.1A CN108795794B (en) | 2017-09-13 | 2017-09-13 | Bacillus pumilus, screening method thereof, preparation method of microbial inoculum and application of microbial inoculum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710821762.1A CN108795794B (en) | 2017-09-13 | 2017-09-13 | Bacillus pumilus, screening method thereof, preparation method of microbial inoculum and application of microbial inoculum |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108795794A CN108795794A (en) | 2018-11-13 |
| CN108795794B true CN108795794B (en) | 2022-02-08 |
Family
ID=64094551
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710821762.1A Expired - Fee Related CN108795794B (en) | 2017-09-13 | 2017-09-13 | Bacillus pumilus, screening method thereof, preparation method of microbial inoculum and application of microbial inoculum |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108795794B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110218677B (en) * | 2019-06-19 | 2022-08-09 | 福建师范大学福清分校 | Bacillus pumilus S1419 and application thereof in potassium dissolution |
| CN110590417B (en) * | 2019-08-01 | 2021-12-17 | 宁夏医科大学 | Bacillus pumilus bacterial fertilizer and application thereof |
| CN110343645B (en) * | 2019-08-09 | 2020-05-22 | 广东省农业科学院蚕业与农产品加工研究所 | Strain bacillus pumilus SEM-7 and application thereof |
| CN111485004B (en) * | 2020-05-07 | 2023-02-24 | 山东农业大学 | Porcine reproductive and respiratory syndrome virus super-susceptible cell line and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102978130A (en) * | 2012-11-01 | 2013-03-20 | 无锡亚克生物科技有限公司 | Bacillus pumilus GBSC66 and its application |
| CN105274026A (en) * | 2015-10-30 | 2016-01-27 | 湖北大学 | Potassium dissolving microorganism and application thereof in crop planting |
| CN105925497A (en) * | 2016-04-20 | 2016-09-07 | 华南农业大学 | Bacillus pumilus and application thereof in decomposing phosphate and potassium and producing acid |
-
2017
- 2017-09-13 CN CN201710821762.1A patent/CN108795794B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102978130A (en) * | 2012-11-01 | 2013-03-20 | 无锡亚克生物科技有限公司 | Bacillus pumilus GBSC66 and its application |
| CN105274026A (en) * | 2015-10-30 | 2016-01-27 | 湖北大学 | Potassium dissolving microorganism and application thereof in crop planting |
| CN105925497A (en) * | 2016-04-20 | 2016-09-07 | 华南农业大学 | Bacillus pumilus and application thereof in decomposing phosphate and potassium and producing acid |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108795794A (en) | 2018-11-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108795794B (en) | Bacillus pumilus, screening method thereof, preparation method of microbial inoculum and application of microbial inoculum | |
| CN103627662B (en) | A kind of Bradyrhizobium sp Arachis and uses thereof | |
| CN106967652B (en) | Rhizobium for promoting growth of common vetch and application of rhizobium | |
| CN111057666B (en) | Korean pseudomonas strain and screening method and application thereof | |
| CN103981102B (en) | DSE bacterial strain 24L-4 and the application on Herba Dendrobii is produced thereof | |
| CN103484396B (en) | New strain of streptomyces thermocarboxydus and application thereof | |
| CN109097303B (en) | Paenibacillus polymyxa, spore suspension of paenibacillus polymyxa, microbial seedling culture substrate and preparation method of microbial seedling culture substrate | |
| CN113943660A (en) | Talaromyces fungus NJAU-L8 for preventing and controlling continuous cropping soil-borne blight and application thereof | |
| CN114381379B (en) | Adhesive film fungus strain TP-8 with dendrobium seedling tillering improving capability and application thereof | |
| CN106520630B (en) | A kind of bacillus megaterium QBJP-F6 and its application | |
| CN109055274B (en) | Caragana rhizobium and fermentation culture method and application thereof | |
| CN108913625B (en) | Salt-tolerant streptomycete, microbial inoculum thereof and application of microbial inoculum thereof in promoting plant growth | |
| CN107467075B (en) | Application of bacillus pumilus as rice growth promoter | |
| CN110218657B (en) | Trichoderma longibrachiatum MD30 and biological organic fertilizer developed by same | |
| CN101914468A (en) | Nitrogen-fixing bacillus megaterium strain DL7 and application thereof | |
| CN110229757B (en) | Trichoderma citrinoviride JS84 capable of effectively promoting crop growth and bio-organic fertilizer developed by trichoderma citrinoviride JS84 | |
| CN103981103A (en) | DSE (Dark Septate Endophyte) strain J-N3 and applications thereof in dendrobium candidum production | |
| CN114958683B (en) | Bacillus and application thereof | |
| CN108203695B (en) | Rhododendron mycorrhizal fungi functional strain and application thereof | |
| CN114395485B (en) | Adhesive film fungus strain TP-2 capable of promoting stem thickness of dendrobium nobile and application | |
| CN105585379A (en) | Biological compound fertilizer and application thereof | |
| CN111117920B (en) | Bacillus mycoides producing protease and siderophore and application thereof | |
| CN114350559A (en) | Salt-tolerant growth-promoting slow-growing rhizobium Liaoning RY6 strain and application thereof | |
| CN110241040B (en) | Korean pseudomonas and application thereof in improving organic nitrogen utilization rate of facility vegetable soil and promoting growth | |
| CN106520629B (en) | One Bacillus species QBJP-F4 and its application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20220208 |