CN108793411B - Short-term near-natural culture method for submerged plants - Google Patents
Short-term near-natural culture method for submerged plants Download PDFInfo
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- CN108793411B CN108793411B CN201810491635.4A CN201810491635A CN108793411B CN 108793411 B CN108793411 B CN 108793411B CN 201810491635 A CN201810491635 A CN 201810491635A CN 108793411 B CN108793411 B CN 108793411B
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01D—MEASURING NOT SPECIALLY ADAPTED FOR A SPECIFIC VARIABLE; ARRANGEMENTS FOR MEASURING TWO OR MORE VARIABLES NOT COVERED IN A SINGLE OTHER SUBCLASS; TARIFF METERING APPARATUS; MEASURING OR TESTING NOT OTHERWISE PROVIDED FOR
- G01D21/00—Measuring or testing not otherwise provided for
- G01D21/02—Measuring two or more variables by means not covered by a single other subclass
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- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
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Abstract
The invention relates to a short-term near-natural culture method of submerged plants, which comprises the following steps: firstly, water taking and water quality index measuring; secondly, treating the dialysis bag and the bag clamp; thirdly, treating broken branches; fourthly, assembling a branch breaking five and connecting and placing a dialysis bag; sixthly, subsequent maintenance, namely, the plant growing in the natural water body is obtained in a dialysis bag culture mode, so that the morphological or physiological indexes of the plant can be measured more accurately; the submerged plants can be separated from microorganisms such as algae, bacteria and the like attached to the submerged plants through the culture of the dialysis bag, but the flowing of nutrition is not influenced, so that the quality of water quality is obtained through the physiological indexes of the plants, and the research and the protection of people's Taihu lake water are facilitated.
Description
Technical Field
The invention relates to a culture method, in particular to a short-term near-natural culture method for submerged plants.
Background
In recent years, with the national importance of environmental protection, especially with the increasingly strengthened protection of ecological environment in Taihu lake and river, water in people's life is an indispensable article, and with the development of modern industry, the quality of water in Taihu lake is increasingly poor, so the research on water quality in Taihu lake becomes an important research direction.
At present, relevant parameters of water quality are obtained by measuring specific indexes of plants produced in natural water; the specific indexes for measuring the plants comprise morphological indexes: plant height, biomass, number of branches, etc.; physiological indexes are as follows: chlorophyll content, starch content etc. and traditional cultivation mode can not be in the isolated culture in natural water, and some microorganism can be attached to the plant, can influence the photosynthesis of plant like this, and nutrition absorbs etc. has caused measured data's inaccurate.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a short-term near-natural submerged plant culture method which can naturally culture submerged plants and accurately measure morphological or physiological indexes of the submerged plants.
In order to achieve the purpose, the invention adopts the technical scheme that: a short-term near-natural culture method for submerged plants comprises the following steps:
first, water intake and water quality index determination
Taking water in a lake, filtering a taken water sample 20L through a No. 25 net, and filtering the filtered water, wherein the filtered water is referred to as WP;
secondly, filtering another 20L of water by a No. 25 net and then a 800-mesh net, wherein the filtered water is hereinafter referred to as NP;
thirdly, at least 250ml of WP and NP are respectively taken by three sampling bottles, TN and TP values are respectively measured by a water quality tester for the WP and NP of the two bottles, and corresponding average values are calculated;
measuring chlorophyll values of the other bottles of WP and NP by using a water quality tester, and calculating an average value;
second, process the dialysis bag and bag clamp
Firstly, knotting 3 circles of fish wire at two ends of the bag clamp, and 48 fish wires are needed; cutting the dialysis bag into 30cm sections, wherein 48 dialysis bags are required;
placing the dialysis bag in a container capable of being boiled, adding water for unfolding and smoothing, and boiling for about one hour if the water completely submerges the dialysis bag;
thirdly, after the water is cooled after the dialysis bags are boiled, washing each dialysis bag in tap water for about 30 seconds;
thirdly, treating the broken branches
Firstly, taking 15cm of broken branches with terminal buds, brushing off attachments on the surfaces of the broken branches by using a toothbrush, soaking the broken branches in 50% alcohol for 5 minutes, immediately changing water twice, washing the alcohol off, and finally slightly absorbing water from each broken branch by using a paper towel;
secondly, weighing the broken branches, recording the weighing number as a hydrilla verticillata when three broken branches are processed, and storing the weighed hydrilla verticillata in water to keep the hydrilla verticillata wet;
fourthly, assembling broken branches
Clamping the treated dialysis bag clamp on a dialysis bag, wherein the distance between the dialysis bag clamp and the bottom end of the dialysis bag is about 2cm, adding not less than 250ml of lake water to ensure that the water quantity does not exceed 2/3 of the dialysis bag after water is filled, placing hydrilla verticillata and label paper with written numbers after the water is filled, sealing the opening by using the dialysis bag clamp to ensure that the upper dialysis bag clamp and the lower dialysis bag clamp are tightly pinched, and storing the dialysis bag in water;
there are 24 groups of hydrilla verticillata 4 eggless in total, so 48 dialysis bags are:
a: 1234 … 1112: black algae WP
131415 … 2324: black algae NP
C, 1234 … 1112: e. grass WP
131415 … 2324: eggera NP;
fifth, connection and placement of dialysis bag
Connecting dialysis bags with the same number in four groups of ABCD by using fish wires, wherein the total number of the dialysis bags is 12, each group of 4 dialysis bags is provided, a plastic bottle is connected to a weight by using a rope, the fish wires connected with the dialysis bags are tied on the rope for fixing the weight, the depth of the dialysis bags is adjusted, the weight can be lifted up when cleaning is needed, the dialysis bags can float on the water surface, and each group of dialysis bags are placed at one point in Taihu lake, and the total number of the dialysis bags is 12;
sixthly, subsequent maintenance
The attachments on the surface of the dialysis bag were wiped every week to observe the number of dialysis bags and whether they were damaged, and were replaced in time.
Preferably, in the water taking and water quality index measurement, when TN and TP are measured on two bottles of WP and NP, the WP and NP need to be pretreated firstly, and the pretreatment comprises filtering by a GF2/C membrane and then refrigerating in a refrigerator.
Due to the application of the technical scheme, compared with the prior art, the invention has the following advantages:
the short-term near-natural cultivation method of the submerged plant mainly obtains the plant growing in the natural water body in a dialysis bag cultivation mode, so that the morphological or physiological index of the plant can be measured more accurately; the submerged plants can be separated from microorganisms such as algae, bacteria and the like attached to the submerged plants through the culture of the dialysis bag, but the flowing of nutrition is not influenced, so that the quality of water quality is obtained through the physiological indexes of the plants, and the research and the protection of people's Taihu lake water are facilitated.
Detailed Description
The present invention will be described in further detail with reference to specific examples.
The invention relates to a short-term near-natural culture method of submerged plants, which comprises the following steps:
first, water intake and water quality index determination
Taking water in a lake, filtering a taken water sample 20L through a No. 25 net, and filtering the filtered water, wherein the filtered water is referred to as WP;
secondly, filtering another 20L of water by a No. 25 net and then a 800-mesh net, wherein the filtered water is hereinafter referred to as NP;
thirdly, at least 250ml of WP and NP are respectively taken by three sampling bottles, TN and TP values are respectively measured by a water quality tester for the WP and NP of the two bottles, and corresponding average values are calculated;
measuring chlorophyll values of the other bottles of WP and NP by using a water quality tester, and calculating an average value;
second, process the dialysis bag and bag clamp
Firstly, knotting 3 circles of fish wire at two ends of the bag clamp, and 48 fish wires are needed; cutting the dialysis bag into 30cm sections, wherein 48 dialysis bags are required;
placing the dialysis bag in a container capable of being boiled, adding water for unfolding and smoothing, and boiling for about one hour if the water completely submerges the dialysis bag;
thirdly, after the water is cooled after the dialysis bags are boiled, washing each dialysis bag in tap water for about 30 seconds;
thirdly, treating the broken branches
Firstly, taking 15cm of broken branches with terminal buds, brushing off attachments on the surfaces of the broken branches by using a toothbrush, soaking the broken branches in 50% alcohol for 5 minutes, immediately changing water twice, washing the alcohol off, and finally slightly absorbing water from each broken branch by using a paper towel;
secondly, weighing the broken branches, recording the weighing number as a hydrilla verticillata when three broken branches are processed, and storing the weighed hydrilla verticillata in water to keep the hydrilla verticillata wet;
fourthly, assembling broken branches
Clamping the treated dialysis bag clamp on a dialysis bag, wherein the distance between the dialysis bag clamp and the bottom end of the dialysis bag is about 2cm, adding not less than 250ml of lake water to ensure that the water quantity does not exceed 2/3 of the dialysis bag after water is filled, placing hydrilla verticillata and label paper with written numbers after the water is filled, sealing the opening by using the dialysis bag clamp to ensure that the upper dialysis bag clamp and the lower dialysis bag clamp are tightly pinched, and storing the dialysis bag in water;
there are 24 groups of hydrilla verticillata 4 eggless in total, so 48 dialysis bags are:
a: 1234 … 1112: black algae WP
131415 … 2324: black algae NP
C, 1234 … 1112: e. grass WP
131415 … 2324: eggera NP
Fifth, connection and placement of dialysis bag
Connecting dialysis bags with the same number in four groups of ABCD by using fish wires, wherein the total number of the dialysis bags is 12, each group of 4 dialysis bags is provided, a plastic bottle is connected to a weight by using a rope, the fish wires connected with the dialysis bags are tied on the rope for fixing the weight, the depth of the dialysis bags is adjusted, the weight can be lifted up when cleaning is needed, the dialysis bags can float on the water surface, and each group of dialysis bags are placed at one point in Taihu lake, and the total number of the dialysis bags is 12;
sixthly, subsequent maintenance
The attachments on the surface of the dialysis bag were wiped every week to observe the number of dialysis bags and whether they were damaged, and were replaced in time.
Furthermore, in the water taking and water quality specification measurement, when TN and TP are measured on two bottles of WP and NP, the WP and NP need to be pretreated firstly, and the pretreatment comprises filtering by a GF2/C membrane and then refrigerating by a refrigerator.
The short-term near-natural cultivation method of the submerged plant mainly obtains the plant growing in the natural water body in a dialysis bag cultivation mode, so that the morphological or physiological index of the plant can be measured more accurately; the submerged plants can be separated from microorganisms such as algae, bacteria and the like attached to the submerged plants through the culture of the dialysis bag, but the flowing of nutrition is not influenced, so that the quality of water quality is obtained through the physiological indexes of the plants, and the research and the protection of people's Taihu lake water are facilitated.
The above is only a specific application example of the present invention, and the protection scope of the present invention is not limited in any way. All the technical solutions formed by equivalent transformation or equivalent replacement fall within the protection scope of the present invention.
Claims (2)
1. A short-term near-natural culture method for submerged plants is characterized by comprising the following steps: the method comprises the following steps:
first, water intake and water quality index determination
Taking water in a lake, filtering a taken water sample 20L through a No. 25 net, and filtering the filtered water, wherein the filtered water is referred to as WP;
secondly, filtering another 20L of water by a No. 25 net and then a 800-mesh net, wherein the filtered water is hereinafter referred to as NP;
thirdly, at least 250ml of WP and NP are respectively taken by using three sampling bottles, 3 bottles of WP and 3 bottles of NP are totally taken, then TN and TP values are respectively measured by using a water quality tester for two bottles of WP and two bottles of NP, and corresponding average values are calculated;
measuring chlorophyll values of the other bottles of WP and NP by using a water quality tester, and calculating an average value;
second, process the dialysis bag and bag clamp
Firstly, knotting 3 circles of fishing line at two ends of the bag clamp, and making 96 fishing lines in total; cutting the dialysis bag into 30cm sections, wherein 48 dialysis bags are required;
placing the dialysis bag in a container capable of being boiled, adding water for unfolding and smoothing, and boiling for one hour when the water completely submerges the dialysis bag;
thirdly, after the water is cooled after the dialysis bags are boiled, washing each dialysis bag for 30 seconds under tap water;
thirdly, treating the broken branches
Firstly, taking 15cm of broken branches with terminal buds, brushing off attachments on the surfaces of the broken branches by using a toothbrush, soaking the broken branches in 50% alcohol for 5 minutes, immediately changing water twice, washing the alcohol off, and finally slightly absorbing water from each broken branch by using a paper towel;
secondly, weighing the broken branches, recording the weighing number as a hydrilla verticillata when three broken branches are processed, and storing the weighed hydrilla verticillata in water to keep the hydrilla verticillata wet;
fourthly, assembling broken branches
Clamping the treated dialysis bag clamp on a dialysis bag, adding lake water of which the volume is not less than 250ml into the dialysis bag clamp at a distance of 2cm from the bottom end of the dialysis bag to ensure that the water volume does not exceed 2/3 of the dialysis bag after water is filled, placing hydrilla verticillata and label paper with written numbers into the dialysis bag clamp, sealing the dialysis bag clamp, ensuring that an upper dialysis bag clamp and a lower dialysis bag clamp are tightly pinched, and storing the dialysis bag in water;
there are 24 groups of hydrilla verticillata and 24 eggplants in total, so 48 dialysis bags are:
a: 1234 … 1112: black algae WP
131415 … 2324: black algae NP
C, 1234 … 1112: e. grass WP
131415 … 2324: eggera NP;
fifth, connection and placement of dialysis bag
Connecting dialysis bags with the same number in four groups of ABCD by using fish wires to form 12 groups in total, wherein each group of 4 dialysis bags is provided with a plastic bottle connected with a weight by using a rope, tying the fish wires connected with the dialysis bags on the rope for fixing the weight, adjusting the depth of the dialysis bags, lifting the weight up when cleaning is needed, enabling the dialysis bags to float on the water surface, and placing each group of dialysis bags at one point in Taihu lake for 12 points in total;
sixthly, subsequent maintenance
The attachments on the surface of the dialysis bag were wiped every week to observe the number of dialysis bags and whether they were damaged, and were replaced in time.
2. The short-term near-natural cultivation method of submerged plants according to claim 1, characterized in that: in the water taking and water quality specification measurement, when TN and TP are measured on two bottles of WP and two bottles of NP, the WP and the NP need to be pretreated firstly, and the pretreatment comprises filtering by a GF2 membrane and then refrigerating by a refrigerator.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810491635.4A CN108793411B (en) | 2018-05-22 | 2018-05-22 | Short-term near-natural culture method for submerged plants |
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|---|---|---|---|
| CN201810491635.4A CN108793411B (en) | 2018-05-22 | 2018-05-22 | Short-term near-natural culture method for submerged plants |
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| Publication Number | Publication Date |
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| CN108793411B true CN108793411B (en) | 2021-04-27 |
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| US8568590B2 (en) * | 2009-05-07 | 2013-10-29 | Phillip Barak | Phosphate recovery from acid phase anaerobic digesters |
| CN103918424A (en) * | 2014-03-04 | 2014-07-16 | 复旦大学 | Planting method of plateau shallow lake submerged plants |
| CN206232492U (en) * | 2016-12-02 | 2017-06-09 | 四川中科水务科技有限公司 | A kind of artificial ecological floating island of purifying of the hypertrophic water system water quality |
| CN106865778B (en) * | 2017-03-24 | 2019-09-27 | 北京林业大学 | A method of it is purified water using the species diversity of submerged plant |
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