CN108786592A - It is a kind of that fixed and temperature control device automatically is carried out in vitro tissue batch - Google Patents
It is a kind of that fixed and temperature control device automatically is carried out in vitro tissue batch Download PDFInfo
- Publication number
- CN108786592A CN108786592A CN201810606413.2A CN201810606413A CN108786592A CN 108786592 A CN108786592 A CN 108786592A CN 201810606413 A CN201810606413 A CN 201810606413A CN 108786592 A CN108786592 A CN 108786592A
- Authority
- CN
- China
- Prior art keywords
- drain
- rifle
- stoste
- formaldehyde
- temperature control
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F29/00—Mixers with rotating receptacles
- B01F29/80—Mixers with rotating receptacles rotating about a substantially vertical axis
- B01F29/81—Mixers with rotating receptacles rotating about a substantially vertical axis with stationary mixing elements
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/20—Measuring; Control or regulation
- B01F35/22—Control or regulation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/30—Driving arrangements; Transmissions; Couplings; Brakes
- B01F35/32—Driving arrangements
- B01F35/325—Driving reciprocating or oscillating stirrers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/50—Mixing receptacles
- B01F35/53—Mixing receptacles characterised by the configuration of the interior, e.g. baffles for facilitating the mixing of components
- B01F35/531—Mixing receptacles characterised by the configuration of the interior, e.g. baffles for facilitating the mixing of components with baffles, plates or bars on the wall or the bottom
- B01F35/5312—Mixing receptacles characterised by the configuration of the interior, e.g. baffles for facilitating the mixing of components with baffles, plates or bars on the wall or the bottom with vertical baffles mounted on the walls
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/71—Feed mechanisms
- B01F35/717—Feed mechanisms characterised by the means for feeding the components to the mixer
- B01F35/7176—Feed mechanisms characterised by the means for feeding the components to the mixer using pumps
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/71—Feed mechanisms
- B01F35/717—Feed mechanisms characterised by the means for feeding the components to the mixer
- B01F35/71805—Feed mechanisms characterised by the means for feeding the components to the mixer using valves, gates, orifices or openings
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/75—Discharge mechanisms
- B01F35/754—Discharge mechanisms characterised by the means for discharging the components from the mixer
- B01F35/7544—Discharge mechanisms characterised by the means for discharging the components from the mixer using pumps
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/75—Discharge mechanisms
- B01F35/754—Discharge mechanisms characterised by the means for discharging the components from the mixer
- B01F35/7547—Discharge mechanisms characterised by the means for discharging the components from the mixer using valves, gates, orifices or openings
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
- B01F35/80—Forming a predetermined ratio of the substances to be mixed
- B01F35/88—Forming a predetermined ratio of the substances to be mixed by feeding the materials batchwise
- B01F35/883—Forming a predetermined ratio of the substances to be mixed by feeding the materials batchwise using flow rate controls for feeding the substances
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
- B01F2101/23—Mixing of laboratory samples e.g. in preparation of analysing or testing properties of materials
Abstract
It is a kind of that fixed and temperature control device automatically is carried out in vitro tissue batch, it is characterized by comprising stoste case for storing (1) and tissue fixed station (2), stoste case for storing (1) is internally provided with water chamber (15), formaldehyde storage chamber (16) and stoste mixing chamber (17), the water chamber (15) is equipped with water injection hole (11), formaldehyde storage chamber (16) is equipped with note formaldehyde hole (12), water chamber (15) is connected with stoste mixing chamber (17) by filler pipe road (13), formaldehyde hole (12) are noted with stoste mixing chamber (17) by adding formaldehyde pipeline (14) to be connected.With liquid automation, intelligent, it is simple to operation to change liquid, and is combined with temperature control, need to select required parameter on a display screen, significantly reduces the burden of experimenter.The fixed batch of tissue carries out, and all attached flow sensor in 20 branches of mixture export, meanwhile, drain rifle is easy to operate.
Description
Technical field
The invention mainly relates in vitro tissue field of experiment equipment, and in particular to a kind of to be carried out automatically in vitro tissue batch
Fixed and temperature control device.
Background technology
In vitro tissue pre-treatment is extremely important in various experiments, and good sample is fixed and preserved to rear
Continuous biochemical analysis influences great.Currently, when sample is fixed, experimenter needs to match certain density fixer manually,
Even timing is needed to replace fixer sometimes.Proportioning, replacement fixer not only increase the workload of experimenter manually, match manually
There is deviation in the poor experimental result that also results in of the solution concentration than caused by, influences the reliability of subsequent experimental result.As can realizing
Automatic mixing will can significantly reduce the workload of experimenter, while can guarantee the consistency of concentration of fixation fluid, further increase reality
Test the reliability of result.Secondly, temperature is also extremely important factor in fixing and subsequently preserving, and too low or excessively high temperature is all
Irreversible influence can be generated on sample.Finally, it is in artificial intelligence, the upsurge of machine learning, carries out image procossing to experiment
It is also essential for person.Therefore, this paper presents one kind being capable of automatic mixing fixer, intelligent temperature control, replacement fixation
The device of liquid and shooting record fixing situation, relates to automatic control technology, PID closed loop intelligent temperature controls technology, image procossing
Technology.
Invention content
Fixed and temperature control device automatically is carried out in vitro tissue batch the present invention provides a kind of, it is possible to reduce experimenter
The time and efforts spent when matching fixer.It is mainly characterized by:Input data is to realize that is automatically controlled shows
Show that plate, semiconductor chilling plate are wrapped in around the cavity of receptacle stand to realize temperature control.
Technical scheme is as follows：
It is a kind of that fixed and temperature control device automatically is carried out in vitro tissue batch, it is characterised in that including stoste case for storing
(1) and tissue fixed station (2), stoste case for storing (1) are internally provided with water chamber (15), formaldehyde storage chamber (16) and stoste mixing
Chamber (17), the water chamber (15) are equipped with water injection hole (11), and formaldehyde storage chamber (16) is equipped with note formaldehyde hole (12), water
Chamber (15) is connected with stoste mixing chamber (17) by filler pipe road (13), and note formaldehyde hole (12) is with stoste mixing chamber (17) by adding
Formaldehyde pipeline (14) is connected, and stoste mixing chamber (17) is also associated with mixture export (18), and tissue fixed station (2) is equipped with for aobvious
The display screen fixing body (21) that display screen is placed, is additionally provided with multiple drain rifle putting holes (27), and each drain rifle putting hole (27) is interior
A drain rifle (22) is placed, drain rifle (22) is equipped with the injection hole (23) of mixed liquor, and drain rifle (22) is lived by drain rifle
Handle (24) connecting bracket (25) is filled in, drain rifle (22) bottom is equipped with fixer and exports (26)；It is set below drain rifle putting hole (27)
There is fixer changing table (3), internal cavity (71) both sides of fixer changing table (7) are completely through placement drawer type holds at this
Device rack (6).
It is renovated (19) by shaft (44) active link on stoste case for storing (1) top；Renovate the trip on (19)
(41) mutually agree with the deck (43) being arranged on stoste case for storing (1), (19) opening and closing can will be renovated by button (42).
The stoste mixing chamber (17) is the blender (3) of funnel type structure, and blender (3) edge is equipped with gear (30),
And it is meshed with the gear being connected to the motor；
Blender (3) top is equipped with entrance (34), and blender (3) lower part is equipped with outlet (32), and blender (3) is internal along mother
Line direction has been uniformly distributed the agitating paddle (35) of long strip type everywhere, and the lid (31) of blender (3) is fixed on gear (30) institute
It in plane, is rotated with gear (30), entrance (34) and filler pipe road (13) and plus formaldehyde pipeline (14) phase of blender (3)
Even；It exports (32) and has disposed solenoid valve, and is connected with mixture export (18).
The drain rifle piston handle (24) of 20 drain rifles can be uniformly controlled, and connecting bracket (25) can be controlled automatically
The movement of piston processed.
The drawer type container rack (6) is corresponding with drain rifle putting hole (27) position.
The mixer outlet (18) has 20 branches, is connected to hose (being not drawn into figure) and is inserted into 20 drain rifles
(22) in the injection hole (23) of mixed liquor.
The drawer type container rack (6) includes external shell (62) and multiple is opened in putting on shell (62)
Set hole (61), be equipped with mating container (5) in putting hole (61), the open edge (51) of container (5) can just frame in putting hole
(61) on.On the cavity wall (61) and cavity bottom (62) of receptacle stand (attached drawing 2), and the cavity wall of placement drain rifle (22)
It may be provided with camera.
The prior art is compared, and the present invention has the following effects that：
1. with liquid automation, intelligent, it is simple to operation to change liquid, and is combined with temperature control, need to select institute on a display screen
Parameter is needed, the burden of experimenter is significantly reduced.
2. the fixed batch of tissue carries out, flow sensor is all attached in 20 branches of mixture export, meanwhile, drain
Rifle is easy to operate, is uniformly controlled to its piston handle, for n parts of (n≤20) tissues provide identical fixed environment with it is consistent pre-
Processing avoids error caused by proportioning manually.
3. vibrating test tube relative to experimenter with mixing liquid, the funnel structure of rotation can make plus internal supplementary structure
Liquid is mixedly more uniform.
4. adding image processing techniques, video is exported using the principles such as infrared, through image procossing, automatic identification and
Whether analysis fixation is abundant, either with or without will organize complete wetting.
Description of the drawings
Fig. 1 is the overall structure diagram of the present invention.
Fig. 2 is the side schematic view of the present invention.
Fig. 3 is in Fig. 2 along A-A sectional views.
Fig. 4 is in Fig. 2 along B-B sectional views.
Fig. 5 is the structural schematic diagram of blender.
Fig. 6 is the structural schematic diagram of drawer type container rack.
Fig. 7 is the structural schematic diagram of blender.
Specific implementation mode
The present invention will be further described below in conjunction with the accompanying drawings.
It is as shown in Figures 1 to 7, a kind of that fixed and temperature control device automatically is carried out in vitro tissue batch, it is characterised in that
Including stoste case for storing (1) and tissue fixed station (2), stoste case for storing (1) is internally provided with water chamber (15), formaldehyde storage
Chamber (16) and stoste mixing chamber (17), the water chamber (15) are equipped with water injection hole (11), and formaldehyde storage chamber (16) is equipped with note
Formaldehyde hole (12), water chamber (15) are connected with stoste mixing chamber (17) by filler pipe road (13), and formaldehyde hole (12) and stoste are noted
For mixing chamber (17) by adding formaldehyde pipeline (14) to be connected, stoste mixing chamber (17) is also associated with mixture export (18), and tissue is solid
Determine platform (2) and be equipped with the display screen fixing body (21) placed for display screen, is additionally provided with multiple drain rifle putting holes (27), Mei Gepai
A drain rifle (22) is placed in liquid rifle putting hole (27), drain rifle (22) is equipped with the injection hole (23) of mixed liquor, drain rifle
(22) by drain rifle piston handle (24) connecting bracket (25), drain rifle (22) bottom is equipped with fixer and exports (26)；Drain rifle
Be equipped with fixer changing table (3) below putting hole (27), internal cavity (71) both sides of fixer changing table (7) completely through,
Drawer type container rack (6) is placed at this.
It is renovated (19) by shaft (44) active link on stoste case for storing (1) top；Renovate the trip on (19)
(41) mutually agree with the deck (43) being arranged on stoste case for storing (1), (19) opening and closing can will be renovated by button (42).
The stoste mixing chamber (17) is the blender (3) of funnel type structure, and blender (3) edge is equipped with gear (30),
And it is meshed with the gear being connected to the motor；
Blender (3) top is equipped with entrance (34), and blender (3) lower part is equipped with outlet (32), and blender (3) is internal along mother
Line direction has been uniformly distributed the agitating paddle (35) of long strip type everywhere, and the lid (31) of blender (3) is fixed on gear (30) institute
It in plane, is rotated with gear (30), entrance (34) and filler pipe road (13) and plus formaldehyde pipeline (14) phase of blender (3)
Even；It exports (32) and has disposed solenoid valve, and is connected with mixture export (18).
The drain rifle piston handle (24) of 20 drain rifles can be uniformly controlled, and connecting bracket (25) can be controlled automatically
The movement of piston processed.
The drawer type container rack (6) is corresponding with drain rifle putting hole (27) position.
The mixer outlet (18) has 20 branches, is connected to hose (being not drawn into figure) and is inserted into 20 drain rifles
(22) in the injection hole (23) of mixed liquor.
The drawer type container rack (6) includes external shell (62) and multiple is opened in putting on shell (62)
Set hole (61), be equipped with mating container (5) in putting hole (61), the open edge (51) of container (5) can just frame in putting hole
(61) on.On the cavity wall (61) and cavity bottom (62) of receptacle stand (attached drawing 2), and the cavity wall of placement drain rifle (22)
It may be provided with camera.
If desired 10 part of 10% formaldehyde fixer, experimenter need to only select required number for 10 on a display screen and
A concentration of 10%, the time needed for required temperature, stirring is fixed.Display screen, which is built-in with microcontroller and can calculate, needs 8ml*10
The formaldehyde of part=80ml and the water of 72ml*10=720ml then pass data to storage water chamber (15) and formaldehyde storage
Water level controller (150) on chamber (16) inner wall and formaldehyde fluid level controller (160) are controlled by measuring the distance that liquid level declines
Flow processed.
Force (forcing) pump and solenoid valve are opened, so that two kinds of liquid is entered mixing chamber (17), and trigger funnel-form mixer and start work
Make.At this point, 10 parts of tissues need to be respectively put into respective container by experimenter, and drawer-like container frame pushing in device is corresponded into position
It sets.After certain time (being calculated according to the amount of solution) to be mixed is automatically closed, funnel bottom valve automatically turns on, in force (forcing) pump
Under auxiliary, homogeneous mixed liquor divides the liquid into drain pump equally by by 10 pipes under valve.Finally open semiconductor system
Cooler realizes temperature control.When tissue fixes beginning, device starts to match fixer next time, in funnel-form after the completion of stirring
It is waited in mixer.Until when needing replacing fixer, directly receptacle stand is taken out, it, can to prevent container interior tissue from flowing out
Whole container frame is wrapped up with a sizeable net, then inclination or container inverted frame, waste liquid is flowed into waste liquid tank.
Only several embodiments of the present invention are expressed for examples detailed above, the description thereof is more specific and detailed, but can not be because
This is interpreted as the limitation to the scope of the claims of the present invention, and there is no necessity and possibility to exhaust all the enbodiments.It should refer to
Go out, all conceiving, within spirit and principle in the present invention, any modification, equivalent substitution, improvement and etc. done should all wrap
Containing within protection scope of the present invention.
Claims (6)
1. a kind of carrying out fixed and temperature control device automatically in vitro tissue batch, it is characterised in that including stoste case for storing
(1) and tissue fixed station (2), stoste case for storing (1) are internally provided with water chamber (15), formaldehyde storage chamber (16) and stoste mixing
Chamber (17), the water chamber (15) are equipped with water injection hole (11), and formaldehyde storage chamber (16) is equipped with note formaldehyde hole (12), water chamber
(15) it is connected by filler pipe road (13) with stoste mixing chamber (17), note formaldehyde hole (12) is with stoste mixing chamber (17) by adding first
Aldehyde pipeline (14) is connected, and stoste mixing chamber (17) is also associated with mixture export (18), and tissue fixed station (2) is equipped with for display
Shield the display screen fixing body (21) placed, is additionally provided with multiple drain rifle putting holes (27), is put in each drain rifle putting hole (27)
A drain rifle (22) is set, drain rifle (22) is equipped with the injection hole (23) of mixed liquor, and drain rifle (22) passes through drain rifle piston
Handle (24) connecting bracket (25), drain rifle (22) bottom are equipped with fixer and export (26)；It is equipped with below drain rifle putting hole (27)
Fixer changing table (3), internal cavity (71) both sides of fixer changing table (7) are completely through placement drawer type container at this
Rack (6).
2. according to claim 1 carry out fixed and temperature control device automatically in vitro tissue batch, it is characterised in that institute
Stoste case for storing (1) top is stated to renovate (19) by shaft (44) active link；Renovate the trip (41) on (19) and setting
Deck (43) on stoste case for storing (1) mutually agrees with, and (19) opening and closing can will be renovated by button (42).
3. according to claim 1 carry out fixed and temperature control device automatically in vitro tissue batch, it is characterised in that institute
The blender (3) that stoste mixing chamber (17) is funnel type structure is stated, blender (3) edge is equipped with gear (30), and and and motor
Connected gear is meshed；Blender (3) top is equipped with entrance (34), and blender (3) lower part is equipped with outlet (32), blender
(3) the internal agitating paddle (35) that long strip type everywhere has been uniformly distributed along generatrix direction, and the lid (31) of blender (3) is fixed on
Plane where gear (30) is rotated with gear (30), entrance (34) and the filler pipe road (13) and plus formaldehyde pipe of blender (3)
Road (14) is connected；It exports (32) and has disposed solenoid valve, and is connected with mixture export (18).
4. according to claim 1 carry out fixed and temperature control device automatically in vitro tissue batch, it is characterised in that institute
It is corresponding with drain rifle putting hole (27) position to state drawer type container rack (6).
5. according to claim 1 carry out fixed and temperature control device automatically in vitro tissue batch, it is characterised in that institute
Stating mixer outlet (18) has 20 branches, is connected to hose (being not drawn into figure) and is inserted into the mixed liquor of 20 drain rifles (22)
In injection hole (23).
6. according to claim 6 carry out fixed and temperature control device automatically in vitro tissue batch, it is characterised in that institute
The shell (62) and multiple putting holes (61) being opened on shell (62) that drawer type container rack (6) includes outside are stated,
Be equipped with mating container (5) in putting hole (61), the open edge (51) of container (5) can just frame on putting hole (61).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810606413.2A CN108786592A (en) | 2018-06-13 | 2018-06-13 | It is a kind of that fixed and temperature control device automatically is carried out in vitro tissue batch |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810606413.2A CN108786592A (en) | 2018-06-13 | 2018-06-13 | It is a kind of that fixed and temperature control device automatically is carried out in vitro tissue batch |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108786592A true CN108786592A (en) | 2018-11-13 |
Family
ID=64085822
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810606413.2A Pending CN108786592A (en) | 2018-06-13 | 2018-06-13 | It is a kind of that fixed and temperature control device automatically is carried out in vitro tissue batch |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108786592A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5259557A (en) * | 1991-09-25 | 1993-11-09 | Ecolab Inc. | Solution proportioner and dispensing system |
| US20030087454A1 (en) * | 2001-10-26 | 2003-05-08 | Schultz Gary A | Method and device for chemical analysis |
| CN101249462A (en) * | 2008-04-08 | 2008-08-27 | 上海理工大学 | Separating gun device |
| CN201205499Y (en) * | 2008-04-02 | 2009-03-11 | 北京达博泰克科技有限公司 | Automatic liquid removing device |
| CN203886475U (en) * | 2014-03-29 | 2014-10-22 | 安徽科技学院 | Automatic slurry stirring device |
| CN107136042A (en) * | 2017-07-17 | 2017-09-08 | 深圳市鼎邦自动化设备有限公司 | Fully automatic liquid medicament is blent and perfusion equipment |
-
2018
- 2018-06-13 CN CN201810606413.2A patent/CN108786592A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5259557A (en) * | 1991-09-25 | 1993-11-09 | Ecolab Inc. | Solution proportioner and dispensing system |
| US20030087454A1 (en) * | 2001-10-26 | 2003-05-08 | Schultz Gary A | Method and device for chemical analysis |
| CN201205499Y (en) * | 2008-04-02 | 2009-03-11 | 北京达博泰克科技有限公司 | Automatic liquid removing device |
| CN101249462A (en) * | 2008-04-08 | 2008-08-27 | 上海理工大学 | Separating gun device |
| CN203886475U (en) * | 2014-03-29 | 2014-10-22 | 安徽科技学院 | Automatic slurry stirring device |
| CN107136042A (en) * | 2017-07-17 | 2017-09-08 | 深圳市鼎邦自动化设备有限公司 | Fully automatic liquid medicament is blent and perfusion equipment |
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Application publication date: 20181113 |