CN106442075A - Fully-automatic liquid mixing and changing device - Google Patents
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- 239000007788 liquid Substances 0.000 title claims abstract description 309
- 238000002156 mixing Methods 0.000 title claims abstract description 18
- 238000002791 soaking Methods 0.000 claims abstract description 71
- 239000002699 waste material Substances 0.000 claims abstract description 40
- 238000003860 storage Methods 0.000 claims abstract description 32
- 238000002360 preparation method Methods 0.000 claims abstract description 31
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- 238000011084 recovery Methods 0.000 claims abstract description 23
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- 239000002912 waste gas Substances 0.000 claims abstract description 7
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- 238000000034 method Methods 0.000 claims description 26
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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Abstract
本发明提供了一种全自动配液换液装置，包括储液装置、配液装置、换液浸泡装置、废液废气回收装置和控制器；配液装置的输入端与储液装置的输出端连接，换液浸泡装置的输入端与配液装置的输出端连接，换液浸泡装置的输出端连接废液废气回收装置的输入端；控制器分别连接储液装置、配液装置、换液浸泡装置和废液废气回收装置；储液装置用于存放原液；配液装置用于将不同的原液按比例定量混合；换液浸泡装置用于存放需要进行处理的生物样本；废液废气回收装置用于收集浸泡生物样本后的各种溶液；控制器用于控制储液装置、配液装置、换液浸泡装置和废液废气回收装置按照设定时间和设定比例来模拟人工实现配液、排液和换液的操作。
The invention provides a fully automatic liquid mixing and changing device, comprising a liquid storage device, a liquid mixing device, a liquid changing soaking device, a waste liquid and waste gas recovery device and a controller; the input end of the liquid mixing device and the output end of the liquid storage device connection, the input end of the liquid changing soaking device is connected to the output end of the liquid dispensing device, the output end of the liquid changing soaking device is connected to the input end of the waste liquid waste gas recovery device; the controller is respectively connected to the liquid storage device, the liquid mixing device, and the liquid changing soaking device device and waste liquid and waste gas recovery device; the liquid storage device is used to store the original liquid; the liquid preparation device is used to quantitatively mix different raw liquids in proportion; the liquid replacement soaking device is used to store biological samples that need to be processed; the waste liquid and gas recovery device is used It is used to collect various solutions after soaking biological samples; the controller is used to control the liquid storage device, liquid preparation device, liquid replacement soaking device and waste liquid and waste gas recovery device to simulate manual liquid preparation and discharge according to the set time and set ratio and liquid exchange operations.
Description
技术领域technical field
本发明属于生物工程技术领域，更具体地，涉及一种全自动配液换液装置。The invention belongs to the technical field of bioengineering, and more specifically relates to a fully automatic liquid mixing and changing device.
背景技术Background technique
在生物工程技术领域中，显微成像是作为观察生物组织的一种基本方法。但是生物组织在离开活的机体后，细胞的结构和组织形态会发生腐败，以至于无法在显微镜下真实的反映出原有的结构和形态。因此，在对生物组织进行显微成像之前，需要使用一系列化学试剂分别对其进行浸泡，以保持生物组织的原有结构和形态。另外，在一些有切片需求的成像仪器中，例如，用电子显微镜或者显微光学断层成像系统进行成像时，还需要对样本进行树脂包埋，从而让生物组织满足精密切削的需求。In the field of bioengineering technology, microscopic imaging is a basic method for observing biological tissues. However, after the biological tissue leaves the living body, the structure and tissue morphology of the cells will be corrupted, so that the original structure and shape cannot be truly reflected under the microscope. Therefore, before microscopic imaging of biological tissue, it is necessary to use a series of chemical reagents to soak it separately, so as to maintain the original structure and shape of biological tissue. In addition, in some imaging instruments that require slicing, for example, when imaging with an electron microscope or a micro-optical tomography system, it is also necessary to embed the sample in resin, so that the biological tissue can meet the requirements of precision cutting.
在上述生物样本的制备过程中，无一例外的都需要使用各种化学试剂对组织进行浸泡处理。而且，为了不破坏生物组织的形态，通常会使用浓度梯度渐变的溶液对样本进行处理，这就使得整个样本的处理过程变的繁琐，复杂，需要在不断的配液换液过程中完成。In the preparation process of the above-mentioned biological samples, without exception, it is necessary to use various chemical reagents to soak the tissues. Moreover, in order not to destroy the morphology of biological tissues, the samples are usually treated with a solution with a gradient concentration, which makes the entire sample processing process cumbersome and complicated, and needs to be completed in the process of continuous liquid preparation and replacement.
例如，在显微光学断层成像系统的样本制备过程中，首先需要用4％的多聚甲醛溶液浸泡样本24小时，然后用PBS溶液浸泡，每隔8小时换液一次，总共三次。接着，分别使用50％，70％，95％的酒精溶液进行梯度浸泡，每2小时更换一次梯度，随后分别使用70％，85％，95％的GMA包埋液对样本进行浸泡，每隔3小时更换一次梯度。然后，将样本放入100％的GMA包埋液中浸泡12小时，最后更换100％的GMA包埋液浸泡3天，完成整个用化学溶液处理样本的过程。For example, in the sample preparation process of a micro-optical tomography system, it is first necessary to soak the sample in 4% paraformaldehyde solution for 24 hours, then soak it in PBS solution, and change the solution every 8 hours, a total of three times. Then, use 50%, 70%, 95% alcohol solution for gradient immersion, replace the gradient every 2 hours, then use 70%, 85%, 95% GMA embedding solution to soak the sample every 3 Change the gradient every hour. Then, put the sample into 100% GMA embedding solution and soak for 12 hours, and finally change the 100% GMA embedding solution and soak for 3 days to complete the whole process of treating the sample with chemical solution.
按照上述的样本制备步骤，整个过程需要进行总共11次配液与换液操作，并且持续时间长达75小时。对于这样繁琐的样品制备过程，目前主要靠人工定时定量的进行操作，效率低，周期长，人力成本高。According to the above-mentioned sample preparation steps, the whole process requires a total of 11 liquid dosing and liquid exchange operations, and the duration is as long as 75 hours. For such a cumbersome sample preparation process, at present, it is mainly operated by manual timing and quantitative operation, which has low efficiency, long cycle and high labor cost.
实验过程中，手动换液与配液的过程会使得苯等各种有毒有机溶剂的挥发，这不仅对长期接触的实验员的健康产生了威胁，而且对生态环境也有一定影响。During the experiment, the process of manual liquid change and liquid preparation will cause the volatilization of various toxic organic solvents such as benzene, which not only poses a threat to the health of long-term contact experimenters, but also has a certain impact on the ecological environment.
对于如此繁琐的操作，仅靠人工操作，很难保证样本制备的成功率。更重要的是很难保证每一批样本制备参数的统一性，不利于实验研究中实验变量的控制，在一定程度上影响了试验数据的有效性。For such a cumbersome operation, it is difficult to guarantee the success rate of sample preparation only by manual operation. More importantly, it is difficult to ensure the uniformity of each batch of sample preparation parameters, which is not conducive to the control of experimental variables in experimental research, and affects the validity of experimental data to a certain extent.
目前市面上已经存在一些对生物组织进行自动脱水或者自动包埋的仪器，但这些仪器主要针对石蜡包埋研发。由于处理过程不同，使用了树脂等一些易挥发，强腐蚀性的有机溶剂，针对石蜡包埋的仪器不能耐这些强腐蚀溶剂，不能自动化的完成树脂包埋样本的前期处理过程。而且，目前市场上并没有可以用于树脂包埋样本过程中，自动配液与换液的仪器。There are already some instruments on the market for automatic dehydration or automatic embedding of biological tissues, but these instruments are mainly developed for paraffin embedding. Due to the different processing procedures, some volatile and highly corrosive organic solvents such as resins are used. The instruments for paraffin embedding are not resistant to these strong corrosive solvents, and cannot automatically complete the pre-treatment process of resin-embedded samples. Moreover, there is currently no instrument that can be used in the process of resin embedding samples for automatic liquid preparation and liquid exchange on the market.
发明内容Contents of the invention
针对现有技术的缺陷，本发明提供了一种全自动配液换液装置；可以实现对强腐蚀性有机溶剂进行自动配液与换液；可以在没有人为干预的情况下，在密闭的环境中按照预先设定的浓度与时间点，进行配液与换液操作。能够自动化的完成树脂包埋样本过程中，使用各种化学溶液对样本进行浸泡的操作，实现了按照统一参数自动化处理样本的过程，提高了样本制备的成功率，与样本制备效率，节省了人力成本。Aiming at the defects of the prior art, the present invention provides a fully automatic liquid dosing and liquid changing device; it can realize automatic liquid dosing and liquid changing for strong corrosive organic solvents; it can be used in a closed environment without human intervention In accordance with the pre-set concentration and time point, the liquid dosing and liquid exchange operations are carried out. It can automatically complete the process of soaking samples in various chemical solutions in the process of resin embedding samples, realizes the process of automatically processing samples according to unified parameters, improves the success rate of sample preparation, and saves manpower cost.
本发明提供了一种全自动配液换液装置，包括储液装置、配液装置、换液浸泡装置、废液废气回收装置和控制器；所述配液装置的输入端与所述储液装置的输出端连接，所述换液浸泡装置的输入端与所述配液装置的输出端连接，所述换液浸泡装置的输出端连接所述废液废气回收装置的输入端；所述控制器分别连接所述储液装置、所述配液装置、所述换液浸泡装置和所述废液废气回收装置；所述储液装置用于存放原液；所述配液装置用于将不同的原液按比例定量混合；所述换液浸泡装置用于存放需要进行处理的生物样本；废液废气回收装置用于收集浸泡生物样本后的各种溶液；所述控制器用于控制所述储液装置、所述配液装置、所述换液浸泡装置和所述废液废气回收装置按照设定时间和设定比例来模拟人工实现配液、排液和换液的操作。The invention provides a fully automatic liquid mixing and changing device, which includes a liquid storage device, a liquid mixing device, a liquid changing soaking device, a waste liquid and waste gas recovery device, and a controller; the input end of the liquid mixing device is connected to the liquid storage The output end of the device is connected, the input end of the liquid changing soaking device is connected to the output end of the liquid distribution device, the output end of the liquid changing soaking device is connected to the input end of the waste liquid and gas recovery device; the control The device is respectively connected to the liquid storage device, the liquid distribution device, the liquid replacement soaking device and the waste liquid waste gas recovery device; the liquid storage device is used to store the original liquid; the liquid distribution device is used to use different The stock solution is quantitatively mixed in proportion; the liquid changing soaking device is used to store biological samples that need to be processed; the waste liquid and gas recovery device is used to collect various solutions soaked in biological samples; the controller is used to control the liquid storage device , the liquid dispensing device, the liquid changing soaking device and the waste liquid and waste gas recovery device simulate the manual operation of liquid dosing, liquid draining and liquid changing according to the set time and set ratio.
更进一步地，所述储液装置包括：多个用于存储配液原材料的原液瓶；在每个原液瓶上设置有一个进气接头和一个出液接头，且所述出液接头在瓶内接一根管到瓶底，用于供泵体吸出原液。Furthermore, the liquid storage device includes: a plurality of stock solution bottles for storing raw materials for liquid preparation; each stock solution bottle is provided with an air inlet joint and a liquid outlet joint, and the liquid outlet joint is inside the bottle Connect a tube to the bottom of the bottle for the pump to suck out the original solution.
更进一步地，所述配液装置包括：一个配液瓶和多个配液泵，所述配液泵的个数与所述原液瓶的个数相同；所述配液瓶通过多个配液泵分别与各个原液瓶相连，用于实现从不同的原液瓶吸取不同的原液来进行配液。Furthermore, the liquid dispensing device includes: a liquid dispensing bottle and a plurality of liquid dispensing pumps, the number of the liquid dispensing pumps is the same as the number of the stock solution bottles; The pumps are respectively connected with each stock solution bottle, and are used to draw different stock solutions from different stock solution bottles for liquid dosing.
更进一步地，所述换液浸泡装置包括：浸泡瓶、排液泵和灌液泵；所述浸泡瓶的输入端通过所述灌液泵与所述配液装置相连，用于实现将配好的液体吸入到所述浸泡瓶中；所述浸泡瓶的输出端通过所述排液泵与所述废液废气回收装置相连，用于实现将浸泡瓶中上次使用的液体排空；所述排液泵和所述灌液泵的控制端均与所述控制器连接，在所述控制器的控制下，通过协调所述排液泵与所述灌液泵，实现换液功能。Furthermore, the soaking device for changing the liquid includes: a soaking bottle, a drain pump and a liquid filling pump; The liquid is sucked into the soaking bottle; the output end of the soaking bottle is connected with the waste liquid and gas recovery device through the liquid discharge pump, and is used to empty the last used liquid in the soaking bottle; Both the control ends of the liquid drainage pump and the filling liquid pump are connected to the controller, and under the control of the controller, the fluid replacement function is realized by coordinating the liquid drainage pump and the filling liquid pump.
更进一步地，所述废液废气回收装置包括：废液瓶和气袋；所述废液瓶用于收集被换掉的溶液；所述气袋通过管材分别与所述原液瓶，所述配液瓶，所述浸泡瓶和所述废液瓶相连，用于保证整个配液换液过程中的气体流通且与外部隔离，防止有毒性的有机气体挥发到空气中。Furthermore, the waste liquid and gas recovery device includes: a waste liquid bottle and an air bag; the waste liquid bottle is used to collect the replaced solution; The soaking bottle is connected to the waste liquid bottle to ensure gas circulation during the entire liquid mixing and changing process and to isolate it from the outside to prevent toxic organic gases from volatilizing into the air.
更进一步地，控制器包括：定量泵控制板，温度传感器，湿度传感器，用于设定配液浓度与换液时间的用户面板；所述温度传感器和所述湿度传感器分别安装在所述浸泡瓶瓶盖上，用于检测所述浸泡瓶工作时的温度与湿度数据并传回给定量泵控制板；定量泵控制板将数据显示到所述用户面板上；用户面板用于供实验员设定不同的配液浓度与换液时间，定量泵控制板会根据用户设定的参数，在设定的时间点，按时序驱动各部分的定量泵，配比设定浓度的溶液，并将浸泡瓶中的溶液替换掉，从而实现自动配液与换液操作。Furthermore, the controller includes: a quantitative pump control board, a temperature sensor, a humidity sensor, and a user panel for setting the concentration of the liquid and the time for changing the liquid; the temperature sensor and the humidity sensor are respectively installed in the soaking bottle On the bottle cap, it is used to detect the temperature and humidity data of the soaking bottle when it is working and send it back to the given quantitative pump control board; the quantitative pump control board displays the data on the user panel; the user panel is used for the experimenter to set Different dosing concentrations and liquid changing times, the quantitative pump control board will drive the quantitative pumps of each part in time sequence according to the parameters set by the user at the set time point, mix the solution with the set concentration, and put the soaking bottle The solution in the liquid is replaced, so as to realize the automatic liquid dosing and liquid exchange operation.
更进一步地，所述配液泵、所述灌液泵或排液泵均为定量泵，所述定量泵包括：单向阀、注射器、腔体夹具、活塞夹具、推杆电机、活动推杆、电机夹具、精密位移传感器、固定底座和第二控制器；所述单向阀为由两个单向阀相连构成的T型单向阀，具有进液口和出液口；所述T型单向阀与所述注射器相连；所述注射器固定于所述腔体夹具上，所述注射器活塞固定于所述活塞夹具上，所述活塞夹具与所述推杆电机的所述活动推杆相连接；所述推杆电机固定于所述电机夹具上；所述精密位移传感器设置在所述活塞夹具与所述电机夹具之间，用于测量推杆的位移；所述腔体夹具与所述电机夹具均固定在所述固定底座上；所述精密位移传感器与所述推杆电机均与所述控制器相连，所述第二控制器根据输入参数计算出电机推杆往复运动的行程和次数，并以精密位移传感器作为反馈，从而实现从进液口精确的按照设置参数泵出定量的液体到出液口。Furthermore, the liquid distribution pump, the liquid filling pump or the liquid discharge pump are quantitative pumps, and the quantitative pumps include: check valves, syringes, cavity clamps, piston clamps, push rod motors, movable push rods , a motor fixture, a precision displacement sensor, a fixed base and a second controller; the one-way valve is a T-type one-way valve formed by connecting two one-way valves, and has a liquid inlet and a liquid outlet; the T-type The one-way valve is connected with the syringe; the syringe is fixed on the cavity fixture, the syringe piston is fixed on the piston fixture, and the piston fixture is connected to the movable push rod of the push rod motor. connected; the push rod motor is fixed on the motor fixture; the precision displacement sensor is arranged between the piston fixture and the motor fixture for measuring the displacement of the push rod; the cavity fixture is connected to the The motor fixtures are all fixed on the fixed base; the precision displacement sensor and the push rod motor are connected to the controller, and the second controller calculates the stroke and times of the reciprocating motion of the motor push rod according to the input parameters , and the precise displacement sensor is used as feedback, so as to realize the precise pumping of a certain amount of liquid from the liquid inlet to the liquid outlet according to the set parameters.
更进一步地，所述单向阀的材料和注射器的材料可以均为玻璃材质，耐腐蚀，可以完成强腐蚀性有机溶剂的输送。Furthermore, the material of the one-way valve and the syringe can be made of glass, which is corrosion-resistant and can complete the delivery of highly corrosive organic solvents.
更进一步地，所述活塞夹具可以通过螺钉与活动推杆固定连接。Furthermore, the piston clamp can be fixedly connected with the movable push rod through screws.
更进一步地，所述T型单向阀通过特氟龙管与所述注射器相连。Furthermore, the T-shaped one-way valve is connected with the syringe through a Teflon tube.
更进一步地，所述推杆电机为步进电机，在第二控制器的控制下，可以驱动推杆电机的推杆伸缩运动。Furthermore, the push rod motor is a stepping motor, and under the control of the second controller, it can drive the push rod of the push rod motor to move telescopically.
更进一步地，所述推杆电机可以通过螺钉与电机夹具紧固在一起。所述腔体夹具与所述电机夹具均可以通过螺钉与所述固定底座紧固在一起。Furthermore, the push rod motor can be fastened together with the motor fixture by screws. Both the cavity fixture and the motor fixture can be fastened together with the fixing base by screws.
相对蠕动泵或者其他形式的泵体，本发明具有更好的耐腐蚀性和更高的定量精度，而且制作成本较低。另外，灵活的可拆卸式设计，可以满足不同的泵液需求。控制系统采用的闭环反馈控制，让泵液的精度有了可靠保证，触摸屏的设计使得使用起来更加方便，更加人性化。Compared with peristaltic pumps or other types of pump body, the present invention has better corrosion resistance and higher quantitative precision, and the manufacturing cost is lower. In addition, the flexible detachable design can meet different pump fluid needs. The closed-loop feedback control adopted by the control system ensures the accuracy of the pump liquid reliably, and the design of the touch screen makes it more convenient and humanized to use.
本发明彻底改变了传统手动配液与换液的繁琐操作过程，自动的通过控制器驱动泵，从储液瓶按照比例抽取液体到配液瓶，然后通过泵抽出浸泡瓶的液体到废液瓶，最后将配液瓶的液体抽到浸泡瓶中，实现了整个配液换液过程的自动化。该装置可以通过用户面板任意设定换液浓度与换液时间节点，可以实从几分钟到数小时间隔的连续定时配液换液操作，极大的提高了样本处理的效率，节省了人力成本。The present invention completely changes the cumbersome operation process of traditional manual liquid preparation and liquid replacement, and automatically drives the pump through the controller to extract liquid from the liquid storage bottle to the liquid preparation bottle in proportion, and then pumps out the liquid in the soaking bottle to the waste liquid bottle , and finally the liquid in the dosing bottle is pumped into the soaking bottle, which realizes the automation of the whole dosing and changing process. The device can arbitrarily set the liquid exchange concentration and liquid exchange time nodes through the user panel, and can realize the continuous timing of liquid dosing and liquid exchange operations from a few minutes to several hours, which greatly improves the efficiency of sample processing and saves labor costs. .
附图说明Description of drawings
图1是本发明实施例提供的全自动配液换液装置的原理框图；Fig. 1 is a functional block diagram of a fully automatic liquid dispensing and changing device provided by an embodiment of the present invention;
图2是本发明实施例提供的全自动配液换液装置的结构图；Fig. 2 is a structural diagram of a fully automatic liquid mixing and changing device provided by an embodiment of the present invention;
图3是本发明实施例提供的全自动配液换液装置中定量泵的结构示意图。Fig. 3 is a schematic structural view of the quantitative pump in the fully automatic liquid mixing and changing device provided by the embodiment of the present invention.
图中：101为储液瓶，201为配液泵，202为锥底试管，203为配液瓶，401为浸泡瓶，402为温度传感器，403为湿度传感器，404为换液泵，301为废液瓶，302未回收气袋，303为排液泵，501为控制器。In the figure: 101 is a liquid storage bottle, 201 is a liquid dispensing pump, 202 is a conical bottom test tube, 203 is a liquid dispensing bottle, 401 is a soaking bottle, 402 is a temperature sensor, 403 is a humidity sensor, 404 is a liquid exchange pump, 301 is a Waste liquid bottle, 302 unrecovered air bag, 303 is a drainage pump, 501 is a controller.
具体实施方式detailed description
为了使本发明的目的、技术方案及优点更加清楚明白，以下结合附图及实施例，对本发明进行进一步详细说明。应当理解，此处所描述的具体实施例仅仅用以解释本发明，并不用于限定本发明。In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.
本发明提供了一种可以实现对强腐蚀性有机溶剂进行自动配液与换液的仪器。可以在没有人为干预的情况下，在密闭的环境中按照预先设定的浓度与时间点，进行配液与换液操作。该仪器能够自动化的完成树脂包埋样本过程中，使用各种化学溶液对样本进行浸泡的操作，实现了按照统一参数自动化处理样本的过程，提高了样本制备的成功率，与样本制备效率，节省了人力成本。The invention provides an instrument capable of automatically dosing and changing liquids for strong corrosive organic solvents. Dosing and changing liquid can be performed in a closed environment according to the preset concentration and time point without human intervention. The instrument can automatically complete the process of soaking samples in various chemical solutions in the process of resin embedding samples, realizes the process of automatically processing samples according to uniform parameters, improves the success rate of sample preparation, and saves human cost.
如图1所示，本发明提供的全自动配液换液装置包括：储液装置1、配液装置2、换液浸泡装置3、废液废气回收装置4和第一控制器5；储液装置主要用来存放原液，通过耐腐蚀的管材与配液装置相连。配液装置主要用来将不同的原液按比例定量混合，它通过换耐腐蚀的管材与浸泡管相连，浸泡装置主要用来存放需要进行处理的生物样本，它通过耐腐蚀管材与废液回收装置相连。废液回收装置主要用来收集浸泡生物样本后的各种溶液。控制器主要用来协调各装置之间的逻辑关系，最终可以让整个装置协调工作，按照设定时间，设定比例，模拟人工实现配液，排液，换液的操作。As shown in Figure 1, the fully automatic liquid preparation and replacement device provided by the present invention includes: a liquid storage device 1, a liquid distribution device 2, a liquid replacement soaking device 3, a waste liquid waste gas recovery device 4 and a first controller 5; The device is mainly used to store the raw liquid, and is connected to the liquid distribution device through a corrosion-resistant pipe. The liquid dispensing device is mainly used to quantitatively mix different raw liquids in proportion. It is connected to the soaking pipe by replacing the corrosion-resistant pipe. The soaking device is mainly used to store biological samples that need to be processed. It passes the corrosion-resistant pipe and the waste liquid recovery device. connected. The waste liquid recovery device is mainly used to collect various solutions soaked in biological samples. The controller is mainly used to coordinate the logical relationship between the various devices, and finally the whole device can work in harmony, according to the set time, set the ratio, and simulate the manual operation of dosing, draining and changing the liquid.
在本发明实施例中，储液装置1包括：若干个原液瓶，用来存储配液原材料。储液瓶盖上有一个进气接头和一个出液接头，出液接头在瓶内接一根管到瓶底，用来供泵体吸出原液。In the embodiment of the present invention, the liquid storage device 1 includes: several stock solution bottles, which are used to store the raw materials for liquid preparation. There is an air inlet joint and a liquid outlet joint on the cap of the liquid storage bottle, and the liquid outlet joint connects a tube in the bottle to the bottom of the bottle, which is used for the pump body to suck out the original liquid.
在本发明实施例中，配液装置2包括：一个配液瓶，与储液瓶数量相同的若干个配液泵。配液瓶通过配液泵与各个储液瓶分别相连，以实现从不同的原液瓶吸取不同的原液来进行配液。In the embodiment of the present invention, the liquid dispensing device 2 includes: a liquid dispensing bottle, and several liquid dispensing pumps having the same number as the liquid storage bottles. The liquid dispensing bottle is connected to each liquid storage bottle respectively through the liquid dosing pump, so as to realize the liquid dosing by absorbing different original liquids from different original liquid bottles.
在本发明实施例中，换液浸泡装置3包括：一个浸泡瓶，一个排液泵，一个灌液泵。浸泡瓶通过排液泵与废液瓶相连，实现将浸泡瓶中上次使用的液体排空。同时浸泡瓶通过灌液泵与配液瓶相连，从而实现将配好的液体吸入到浸泡瓶中。通过排液泵与灌液泵的协调，实现换液功能。In the embodiment of the present invention, the liquid changing soaking device 3 includes: a soaking bottle, a liquid draining pump, and a liquid filling pump. The immersion bottle is connected to the waste liquid bottle through a liquid discharge pump to realize the emptying of the last used liquid in the immersion bottle. At the same time, the soaking bottle is connected with the liquid preparation bottle through the liquid filling pump, so that the prepared liquid can be sucked into the soaking bottle. Through the coordination of the drain pump and the filling pump, the function of changing the liquid is realized.
在本发明实施例中，废液废气回收装置4包括：一个废液瓶，一个气袋。气袋与所述原液瓶，配液瓶，浸泡瓶以及废液瓶通过管材相连。废液瓶用来收集被换掉的溶液。气袋可以保证整个配液换液过程中的气体流通与外部隔离，以防止有毒性的有机气体挥发到空气中。In the embodiment of the present invention, the waste liquid and gas recovery device 4 includes: a waste liquid bottle and an air bag. The air bag is connected with the stock solution bottle, the liquid preparation bottle, the soaking bottle and the waste liquid bottle through pipes. The waste bottle is used to collect the replaced solution. The air bag can ensure the gas circulation during the whole liquid mixing and changing process and isolate it from the outside, so as to prevent the toxic organic gas from volatilizing into the air.
在本发明实施例中，第一控制器5包括：一块带有微处理器的定量泵控制板，一个温度传感器，一个湿度传感器，一个设定配液浓度与换液时间的用户面板。温度传感器与湿度传感器分别安装在浸泡瓶瓶盖上，用来检测浸泡瓶时时温度与湿度数据并传回给微处理器，微处理器将数据显示到用户面板上。用户设定参数的面板，可以供实验员设定不同的配液浓度与换液时间，微处理器会根据用户设定的参数，在设定的时间点，按时序驱动各部分的定量泵，配比设定浓度的溶液，并将浸泡瓶中的溶液替换掉，从而实现自动配液与换液操作。In the embodiment of the present invention, the first controller 5 includes: a quantitative pump control board with a microprocessor, a temperature sensor, a humidity sensor, and a user panel for setting the dosing concentration and liquid changing time. The temperature sensor and the humidity sensor are installed on the cap of the soaking bottle respectively, and are used to detect the temperature and humidity data of the soaking bottle and send them back to the microprocessor, which displays the data on the user panel. The user-set parameter panel can be used by the experimenter to set different dosing concentrations and liquid replacement time. The microprocessor will drive the quantitative pumps of each part in time sequence according to the parameters set by the user. Proportionate the solution with the set concentration, and replace the solution in the soaking bottle, so as to realize the operation of automatic liquid dosing and liquid replacement.
本发明彻底改变了传统手动配液与换液的繁琐操作过程，自动的通过控制器驱动泵，从储液瓶按照比例抽取液体到配液瓶，然后通过泵抽出浸泡瓶的液体到废液瓶，最后将配液瓶的液体抽到浸泡瓶中，实现了整个配液换液过程的自动化。该装置可以通过用户面板任意设定换液浓度与换液时间节点，可以实从几分钟到数小时间隔的连续定时配液换液操作，极大的提高了样本处理的效率，节省了人力成本。The present invention completely changes the cumbersome operation process of traditional manual liquid preparation and liquid replacement, and automatically drives the pump through the controller to extract liquid from the liquid storage bottle to the liquid preparation bottle in proportion, and then pumps out the liquid in the soaking bottle to the waste liquid bottle , and finally the liquid in the dosing bottle is pumped into the soaking bottle, which realizes the automation of the whole dosing and changing process. The device can arbitrarily set the liquid exchange concentration and liquid exchange time nodes through the user panel, and can realize the continuous timing of liquid dosing and liquid exchange operations from a few minutes to several hours, which greatly improves the efficiency of sample processing and saves labor costs. .
再者，实验员可以通过用户面板设计好实验参数，精确的控制浸泡溶液的浓度与浸泡时间，可以很方便的设计各种不同实验条件，完成以前由于复杂人工操作而很难完成的渗透实验。Furthermore, the experimenter can design the experimental parameters through the user panel, accurately control the concentration of the soaking solution and the soaking time, and can easily design various experimental conditions to complete the penetration experiment that was difficult to complete due to complicated manual operations.
再者，浸泡瓶中装配有温度与湿度传感器，实验员可以从方便的从触摸屏上获取样本浸泡环境的温度与湿度参数，从而保证实验参数的统一性。Furthermore, the soaking bottle is equipped with temperature and humidity sensors, and the experimenter can easily obtain the temperature and humidity parameters of the sample soaking environment from the touch screen, so as to ensure the uniformity of the experimental parameters.
再者，该装置通过管道和泵体输送液体，并且装置上的出气口与进气口均连接在缓冲气袋上，实现了密闭操作，因此，该装置可以有效的防止一些有毒有机溶剂挥发到空气中，避免对实验员身体健康以及环境产生有害影响。Furthermore, the device transports the liquid through the pipeline and the pump body, and the air outlet and the air inlet on the device are connected to the buffer air bag to realize airtight operation. Therefore, the device can effectively prevent some toxic organic solvents from volatilizing to the In the air, avoid harmful effects on the health of the experimenters and the environment.
现在结合附图对本发明作进一步详细说明。这些图均为简化示意图，仅以示意方式说明本发明的基本结构。The present invention is described in further detail in conjunction with accompanying drawing now. These figures are all simplified schematic diagrams, and only illustrate the basic structure of the present invention in a schematic manner.
如图2所示，一种自动配液换液装置，包括带螺纹密封盖的储液瓶101，储液瓶101用于存放配液原液，例如图示的四个储液瓶，分别存放水，酒精，二甲苯，树脂单体，待原液消耗殆尽时，可以拧开带螺纹的密封盖，补给相同原液。带螺纹的密封盖为耐有机溶剂的PE塑料，瓶盖上有两个接头，一个接头通过穿板塑料接头接排液管，排液管采用耐腐蚀的特氟龙材质硬管，排液管在瓶内的管口与瓶底处于一个平面，另一个接头通过穿板塑料接头接进气管，进气管管口稍低于瓶口。As shown in Figure 2, an automatic liquid dispensing and changing device includes a liquid storage bottle 101 with a threaded sealing cap, and the liquid storage bottle 101 is used to store the original solution of the liquid preparation, such as the four liquid storage bottles shown in the figure, which store water respectively. , Alcohol, xylene, resin monomer, when the stock solution is exhausted, you can unscrew the threaded sealing cap to replenish the same stock solution. The threaded sealing cap is made of organic solvent-resistant PE plastic. There are two joints on the bottle cap. One joint is connected to the drain pipe through the plastic joint through the plate. The drain pipe is made of corrosion-resistant Teflon hard tube. The nozzle in the bottle is on a plane with the bottom of the bottle, and the other joint is connected to the air inlet pipe through a plate-through plastic joint, and the nozzle of the air inlet pipe is slightly lower than the bottleneck.
四个储液瓶101的排液管分别与四个耐腐蚀配液泵201相连，四个配液泵201的出液口通过特氟龙硬管直接与配液瓶203的进液口上。配液瓶203作为一个缓冲瓶，用来混合不同的溶质与溶剂。与储液瓶101相同，配液瓶203也采用了带螺纹的密封盖，瓶盖上有四个进液接头一个出液接头与一个进气或出气接头。配液瓶内设计有一个锥形的试管202，该锥形试管202采用了耐腐蚀的PE塑料，锥形的底面可以保证排液不残留。The discharge pipes of the four liquid storage bottles 101 are respectively connected to the four corrosion-resistant liquid distribution pumps 201 , and the liquid outlets of the four liquid distribution pumps 201 are directly connected to the liquid inlets of the liquid distribution bottles 203 through Teflon hard tubes. The liquid preparation bottle 203 is used as a buffer bottle for mixing different solutes and solvents. Same as the liquid storage bottle 101, the liquid dispensing bottle 203 also adopts a threaded sealing cap, and the bottle cap has four liquid inlet connectors, one liquid outlet connector and one air inlet or gas outlet connector. A conical test tube 202 is designed inside the liquid dispensing bottle. The conical test tube 202 is made of corrosion-resistant PE plastic, and the conical bottom surface can ensure that the draining liquid does not remain.
配液瓶203中的排液管与一个灌液泵404相连，该泵的出液口与浸泡瓶401的进液接头相连，浸泡瓶401基本与配液瓶相同，包括一个带螺纹的密封盖和内部的锥底试管。另外，浸泡瓶401中还设计有湿度传感器403与温度传感器402，这两个传感器均与控制器相连，将采集的温度与湿度参数反馈给第一控制器501，然后控制器将数据显示在触摸屏上。浸泡瓶401的瓶盖上设计有一个进液接头，一个出液接头与一个进气或出气接头。The liquid discharge pipe in the dosing bottle 203 is connected to a liquid filling pump 404, and the liquid outlet of the pump is connected to the liquid inlet joint of the soaking bottle 401. The soaking bottle 401 is basically the same as the dosing bottle, including a threaded sealing cap and inner conical bottom test tubes. In addition, the soaking bottle 401 is also designed with a humidity sensor 403 and a temperature sensor 402. These two sensors are connected to the controller, and the collected temperature and humidity parameters are fed back to the first controller 501, and then the controller displays the data on the touch screen. superior. The bottle cap of the soaking bottle 401 is designed with a liquid inlet joint, a liquid outlet joint and an air inlet or air outlet joint.
浸泡瓶401中的排液管与一个排液泵405相连，该泵的出液口与废液瓶301的进液接头相接。废液瓶301是用来储存浸泡瓶401中排出液体的容器，废液瓶301的瓶盖上有一个进液接头和一个出气接头。The drain pipe in the soaking bottle 401 is connected with a drain pump 405 , and the liquid outlet of the pump is connected with the liquid inlet connector of the waste liquid bottle 301 . The waste liquid bottle 301 is a container for storing the liquid discharged from the soaking bottle 401, and the bottle cap of the waste liquid bottle 301 has a liquid inlet connector and an air outlet connector.
在上述结构中，所有容器的出气或进气接头均与一个气体缓冲袋302相连。从而形成一个密闭的环境，防止溶液挥发到环境中。In the above structure, the gas outlet or inlet joints of all containers are connected with a gas buffer bag 302 . Thereby forming a closed environment to prevent the solution from volatilizing into the environment.
在本发明实施例中，配液泵201，灌液泵404，排液泵405均为一种耐腐蚀的定量泵，结构如图3所示。该定量泵包括：T型单向阀6采用了玻璃材质，并通过一段特氟龙管与同样是玻璃材质的注射器7相连。注射器7固定于腔体夹具8上，注射器7活塞固定于活塞夹具9上，活塞夹具9与推杆电机10的活动推杆11接相连，并通过螺钉固定。推杆电机10固定于电机夹具12上。在活塞夹具9与电机夹具12之间，安装了一个精密位移传感器13，用来测量的推杆的位移。其中，精密位移传感器13可以采用高精度电阻尺。腔体夹具与推杆电机夹具均固定在一块固定底座14上。In the embodiment of the present invention, the dosing pump 201 , the filling pump 404 , and the draining pump 405 are all corrosion-resistant quantitative pumps, the structure of which is shown in FIG. 3 . The quantitative pump includes: a T-shaped one-way valve 6 made of glass, and connected to a syringe 7 also made of glass through a section of Teflon tube. The syringe 7 is fixed on the cavity fixture 8, the piston of the syringe 7 is fixed on the piston fixture 9, and the piston fixture 9 is connected to the movable push rod 11 of the push rod motor 10 and fixed by screws. The push rod motor 10 is fixed on the motor fixture 12 . Between the piston clamp 9 and the motor clamp 12, a precision displacement sensor 13 is installed to measure the displacement of the push rod. Wherein, the precise displacement sensor 13 can adopt a high-precision resistance scale. The cavity clamp and the push rod motor clamp are all fixed on a fixed base 14.
本发明的具体工作过程如下：实验员首先拧开浸泡瓶的瓶盖，放入样品到锥底试管中，然后通过触摸屏，设置换液浓度与换液间隔时间，例如50％酒精与水混合物浸泡2小时，然后50％酒精与二甲苯混合物浸泡2小时，然后50％的树脂单体与二甲苯混合物浸泡2小时，然后设置换液量，例如20ml。设置好参数后，点击屏幕上的开始按钮，装置开始计时工作。首先，控制器驱动储液瓶1-1对应的定量泵抽取10ml水到配液瓶7中，然后控制器再驱动储液瓶1-2对应的定量泵取10ml酒精到配液瓶中。接下来，控制器驱动浸泡瓶对应的定量泵泵将浸泡瓶中的溶液全部抽取到废液瓶中。抽取完毕后，控制驱动配液瓶对应的定量泵将配液瓶中20ml的50％的酒精到浸泡瓶中。到此，装置自动完成一次配液换液流程。根据设定的参数，控制器在计时2小时后，会按照上述配液，排液，灌液的流程控制不同的泵实现50％二甲苯与酒精混合液的配液与换液操作。依次，在间隔2小时后，控制器控制泵进行类似动作，完成50％树脂单体的配液与换液操作。到此，装置完成了整个配液换液工作。The specific working process of the present invention is as follows: the experimenter first unscrews the bottle cap of the soaking bottle, puts the sample into the test tube at the bottom of the cone, and then through the touch screen, sets the concentration and interval of changing the liquid, such as soaking in a mixture of 50% alcohol and water 2 hours, then soak in a mixture of 50% alcohol and xylene for 2 hours, then soak in a mixture of 50% resin monomer and xylene for 2 hours, and then set the liquid replacement volume, for example 20ml. After setting the parameters, click the start button on the screen, and the device will start timing. First, the controller drives the quantitative pump corresponding to the liquid storage bottle 1-1 to pump 10ml of water into the dosing bottle 7, and then the controller drives the quantitative pump corresponding to the liquid storage bottle 1-2 to pump 10ml of alcohol into the dosing bottle. Next, the controller drives the quantitative pump corresponding to the soaking bottle to pump all the solution in the soaking bottle into the waste liquid bottle. After the extraction is completed, control and drive the quantitative pump corresponding to the dosing bottle to put 20ml of 50% alcohol in the dosing bottle into the soaking bottle. At this point, the device automatically completes a liquid dosing and liquid replacement process. According to the set parameters, after 2 hours of timing, the controller will control different pumps according to the above-mentioned process of dosing, draining and filling to realize the dosing and changing operation of 50% xylene and alcohol mixture. Sequentially, after an interval of 2 hours, the controller controls the pump to perform similar actions to complete the liquid dosing and liquid exchange operations of 50% of the resin monomer. At this point, the device has completed the entire liquid preparation and replacement work.
该具体实施案例中的装置，可以设置60个不同的换液阶段，完全可以满足现有的实验要求，而且，该装置可以准时的进行配液换液操作，极大的保证了样本制备参数的统一性，提高了了样本制备的成功率。而且，自动化的操作很大程度的节省了人力成本。另外，相比手动操作，装置本身的密闭操作有效的避免了对实验员健康的影响和对环境的伤害。The device in this specific implementation case can be set to 60 different stages of liquid exchange, which can fully meet the existing experimental requirements. Moreover, the device can perform liquid dosing and liquid exchange operations on time, which greatly ensures the accuracy of sample preparation parameters. Uniformity improves the success rate of sample preparation. Moreover, automated operations greatly save labor costs. In addition, compared with manual operation, the airtight operation of the device itself effectively avoids the impact on the health of the experimenter and the harm to the environment.
本领域的技术人员容易理解，以上所述仅为本发明的较佳实施例而已，并不用以限制本发明，凡在本发明的精神和原则之内所作的任何修改、等同替换和改进等，均应包含在本发明的保护范围之内。It is easy for those skilled in the art to understand that the above descriptions are only preferred embodiments of the present invention, and are not intended to limit the present invention. Any modifications, equivalent replacements and improvements made within the spirit and principles of the present invention, All should be included within the protection scope of the present invention.
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