CN108785341A - A kind of preparation method of purslane extract - Google Patents
A kind of preparation method of purslane extract Download PDFInfo
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- CN108785341A CN108785341A CN201810982839.8A CN201810982839A CN108785341A CN 108785341 A CN108785341 A CN 108785341A CN 201810982839 A CN201810982839 A CN 201810982839A CN 108785341 A CN108785341 A CN 108785341A
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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Abstract
The present invention relates to a kind of preparation methods of purslane extract, it includes the following steps：（1）, prepare raw material purslane herb, impurity elimination, cleaning, 40-60 mesh is crushed to after drying, obtains purslane powder；（2）, weigh purslane powder, it is 70%-95% ethanol solutions that 8-10 times of volume fraction measured is added into purslane powder, in temperature be at 45-60 DEG C extraction time 20-40 minute extract, extracting solution is concentrated under reduced pressure into no alcohol taste and obtains concentrate by acquisition extracting solution；（3）, in step（2）It is added distilled water in obtained concentrate, the mass ratio of concentrate and distilled water is 1：（4-8）, decoct 2-3 hours, centrifugation obtains supernatant；（4）, by step（3）Obtained supernatant enters progress micro-filtration in microfiltration membranes and obtains purslane extract；This method can high yield the higher purslane extract of obtained active constituent content, be suitable for industrialization.
Description
Technical field
The invention belongs to technical field of extraction of Chinese traditional medicine, and in particular to a kind of preparation method of purslane extract.
Background technology
Purslane is Portulacaceae annual herb plant, and various regions are distributed at home.It is medical and edible dual purpose plant, entirely
Careless hyoscine has clearing heat and promoting diuresis, removing toxicity for detumescence, anti-inflammatory, quenches the thirst, diuresis；Seed improving eyesight.
Since purslane for natural origin and has the above pharmacologic action, it will be highly effective and endanger very
Small bacteriostatic agent.In field of medicaments, the usage amount of antibiotic can be reduced using this natural bacteriostatic agent；In field of food or
Cosmetic field can substitute the synthesis chemistry bacteriostatic agent and preservative generally used now with this natural bacteriostatic agent.
The crude drug presence of purslane is not easy to maintain, it is inconvenient for use the problems such as.Therefore, it is necessary to be carried out to purslane crude drug
Further processing, such as extracted active constituent therein by solvent extraction；Solvent extraction process master used at present
To include organic solvent extraction and water extraction.
Organic solvent extracting method is more common extracting method, is made using ethyl alcohol, methanol or other organic solvents
It is extracted for solvent.Its advantage is that higher yield can be obtained, and product has higher purity.However, being answered certain
In production with field, such as organic food, drug, cosmetics etc., due to relevant law or the regulation of regulation, organic solvent
Using being severely restricted, therefore it is unsuitable for these applications using the purslane extract that organic solvent extracting method produces.
Purslane raw material is only placed in water by conventional water extracting method, at normal temperatures or under conditions of heating, is being stirred
Or extracted under conditions of not stirring, such extracting method extraction efficiency is relatively low.
Invention content
The present invention is directed to the shortcomings of the prior art, provides a kind of preparation method of purslane extract, should
Method can keep the globality of purslane substance group, keep the consistency of drug effect, and process conditions are simple, be easy to industry
Change, the quality of production is easy to control, of low cost, and using effect is good.
The purpose of the present invention is what is realized in the following manner：
The present invention provides a kind of preparation methods of purslane extract, it includes the following steps：
（1）, prepare raw material purslane herb, impurity elimination, cleaning, 40-60 mesh is crushed to after drying, obtains purslane powder；
（2）, weigh purslane powder, it is 70%-95% ethanol solutions that 8-10 times of volume fraction measured is added into purslane powder,
In temperature be 45-60 DEG C at extraction time 20-40 minute extract, acquisition extracting solution, extracting solution is concentrated under reduced pressure into no alcohol
Taste obtains concentrate；
（3）, in step（2）It is added distilled water in obtained concentrate, the mass ratio of concentrate and distilled water is 1：（4-8）, decoct
It boils 2-3 hours, decoction liquor is centrifuged with 3000-3500rpm, obtains supernatant；
（4）, by step（3）Obtained supernatant enters progress micro-filtration in microfiltration membranes and obtains purslane extract.
According to the preparation method of above-mentioned purslane extract, step（2）In, 8 times of amounts of addition into purslane powder
Volume fraction be 75% ethanol solution, in temperature be 50 DEG C at 30 minutes extraction times extract, obtain extracting solution, will extract
Liquid is concentrated under reduced pressure into no alcohol taste and obtains concentrate.
According to the preparation method of above-mentioned purslane extract, step（3）In, in step（2）Add in obtained concentrate
Enter distilled water, the mass ratio of concentrate and distilled water is 1：6.
According to the preparation method of above-mentioned purslane extract, step（4）Middle micro-filtration filter membrane is 19 channel microfiltration membranes, internal diameter
8mm, outer diameter 12mm, pipe range 1000mm, 0.2 μm, membrane material Al2O3, membrane area 0.4m2 of film average pore size, operation pressure difference control
In 0.01-0.2MPa.
Compared with prior art, beneficial effects of the present invention：
1, purslane extract refreshing and detoxicating produced by the present invention reduces animal disease；Reduce animal diarrhea incidence, enhancing
Body's immunity promotes growth；Palatability is strong, improves feed intake；And natural, green, without any chemicals or swash
Element noresidue, has no drug resistance, has no toxic side effect.
2, the present invention is easy to operate, and the active constituent yield of extraction is high, retains the active ingredient of purslane, and recovery rate is high,
Moisture is low, can make full use of raw material resources, is very suitable for industrialized production.
Specific implementation mode
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation
Example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common
The every other embodiment that technical staff is obtained without creative efforts belongs to the model that the present invention protects
It encloses.
Embodiment 1：
The present invention provides a kind of preparation methods of purslane extract, it includes the following steps：
（1）, prepare raw material purslane herb, impurity elimination, cleaning, 40 mesh are crushed to after drying, obtain purslane powder；
（2）, weigh purslane powder, into purslane powder be added 8 times amount volume fractions be 75% ethanol solution, in temperature
Be at 50 DEG C 30 minutes extraction times extract, obtain extracting solution, extracting solution is concentrated under reduced pressure into no alcohol taste and obtains concentrate；
（3）, in step（2）It is added distilled water in obtained concentrate, the mass ratio of concentrate and distilled water is 1：6, decoct 2.5
Hour, decoction liquor is centrifuged with 3000rpm, obtains supernatant；
（4）, by step（3）Obtained supernatant enters progress micro-filtration in microfiltration membranes and obtains purslane extract, and micro-filtration filter membrane is 19
Channel microfiltration membranes, internal diameter 8mm, outer diameter 12mm, pipe range 1000mm, 0.2 μm of film average pore size, membrane material Al2O3, membrane area
0.4m2, operation pressure difference control is in 0.01-0.2MPa.
Embodiment 2：
The present invention provides a kind of preparation methods of purslane extract, it includes the following steps：
（1）, prepare raw material purslane herb, impurity elimination, cleaning, 60 mesh are crushed to after drying, obtain purslane powder；
（2）, weigh purslane powder, into purslane powder be added 9 times amount volume fractions be 95% ethanol solution, in temperature
Be at 45 DEG C 40 minutes extraction times extract, obtain extracting solution, extracting solution is concentrated under reduced pressure into no alcohol taste and obtains concentrate；
（3）, in step（2）It is added distilled water in obtained concentrate, the mass ratio of concentrate and distilled water is 1：4, it is small to decoct 3
When, decoction liquor is centrifuged with 3500rpm, obtains supernatant；
（4）, by step（3）Obtained supernatant enters progress micro-filtration in microfiltration membranes and obtains purslane extract, and micro-filtration filter membrane is 19
Channel microfiltration membranes, internal diameter 8mm, outer diameter 12mm, pipe range 1000mm, 0.2 μm of film average pore size, membrane material Al2O3, membrane area
0.4m2, operation pressure difference control is in 0.01-0.2MPa.
Embodiment 3：
The present invention provides a kind of preparation methods of purslane extract, it includes the following steps：
（1）, prepare raw material purslane herb, impurity elimination, cleaning, 40 mesh are crushed to after drying, obtain purslane powder；
（2）, weigh purslane powder, into purslane powder be added 8 times amount volume fractions be 70% ethanol solution, in temperature
Be at 60 DEG C 25 minutes extraction times extract, obtain extracting solution, extracting solution is concentrated under reduced pressure into no alcohol taste and obtains concentrate；
（3）, in step（2）It is added distilled water in obtained concentrate, the mass ratio of concentrate and distilled water is 1：7, it is small to decoct 2
When, decoction liquor is centrifuged with 3200rpm, obtains supernatant；
（4）, by step（3）Obtained supernatant enters progress micro-filtration in microfiltration membranes and obtains purslane extract, and micro-filtration filter membrane is 19
Channel microfiltration membranes, internal diameter 8mm, outer diameter 12mm, pipe range 1000mm, 0.2 μm of film average pore size, membrane material Al2O3, membrane area
0.4m2, operation pressure difference control is in 0.01-0.2MPa.
Embodiment 4：
The present invention provides a kind of preparation methods of purslane extract, it includes the following steps：
（1）, prepare raw material purslane herb, impurity elimination, cleaning, 60 mesh are crushed to after drying, obtain purslane powder；
（2）, weigh purslane powder, into purslane powder be added 8 times amount volume fractions be 85% ethanol solution, in temperature
Be at 45-60 DEG C extraction time 20-40 minute extract, obtain extracting solution, by extracting solution be concentrated under reduced pressure into no alcohol taste obtain it is dense
Contracting liquid；
（3）, in step（2）It is added distilled water in obtained concentrate, the mass ratio of concentrate and distilled water is 1：8, it is small to decoct 3
When, decoction liquor is centrifuged with 3500rpm, obtains supernatant；
（4）, by step（3）Obtained supernatant enters progress micro-filtration in microfiltration membranes and obtains purslane extract, and micro-filtration filter membrane is 19
Channel microfiltration membranes, internal diameter 8mm, outer diameter 12mm, pipe range 1000mm, 0.2 μm of film average pore size, membrane material Al2O3, membrane area
0.4m2, operation pressure difference control is in 0.01-0.2MPa.
Embodiment 5：
The present invention provides a kind of preparation methods of purslane extract, it includes the following steps：
（1）, prepare raw material purslane herb, impurity elimination, cleaning, 40 mesh are crushed to after drying, obtain purslane powder；
（2）, weigh purslane powder, into purslane powder be added 8.5 times amount volume fractions be 75% ethanol solution, Yu Wen
Degree extracts for 30 minutes extraction times at 60 DEG C, obtains extracting solution, extracting solution is concentrated under reduced pressure into no alcohol taste and obtains concentrate；
（3）, in step（2）It is added distilled water in obtained concentrate, the mass ratio of concentrate and distilled water is 1：5, decoct 2.5
Hour, decoction liquor is centrifuged with 3000rpm, obtains supernatant；
（4）, by step（3）Obtained supernatant enters progress micro-filtration in microfiltration membranes and obtains purslane extract, and micro-filtration filter membrane is 19
Channel microfiltration membranes, internal diameter 8mm, outer diameter 12mm, pipe range 1000mm, 0.2 μm of film average pore size, membrane material Al2O3, membrane area
0.4m2, operation pressure difference control is in 0.01-0.2MPa.
Embodiment 6：
The present invention provides a kind of preparation methods of purslane extract, it includes the following steps：
（1）, prepare raw material purslane herb, impurity elimination, cleaning, 40 mesh are crushed to after drying, obtain purslane powder；
（2）, weigh purslane powder, into purslane powder be added 8 times amount volume fractions be 95% ethanol solution, in temperature
Be at 55 DEG C 35 minutes extraction times extract, obtain extracting solution, extracting solution is concentrated under reduced pressure into no alcohol taste and obtains concentrate；
（3）, in step（2）It is added distilled water in obtained concentrate, the mass ratio of concentrate and distilled water is 1：7, decoct 2.5
Hour, decoction liquor is centrifuged with 3500rpm, obtains supernatant；
（4）, by step（3）Obtained supernatant enters progress micro-filtration in microfiltration membranes and obtains purslane extract, and micro-filtration filter membrane is 19
Channel microfiltration membranes, internal diameter 8mm, outer diameter 12mm, pipe range 1000mm, 0.2 μm of film average pore size, membrane material Al2O3, membrane area
0.4m2, operation pressure difference control is in 0.01-0.2MPa.
Embodiment 7：
The present invention provides a kind of preparation methods of purslane extract, it includes the following steps：
（1）, prepare raw material purslane herb, impurity elimination, cleaning, 60 mesh are crushed to after drying, obtain purslane powder；
（2）, weigh purslane powder, into purslane powder be added 9 times amount volume fractions be 95% ethanol solution, in temperature
Be at 45 DEG C 25 minutes extraction times extract, obtain extracting solution, extracting solution is concentrated under reduced pressure into no alcohol taste and obtains concentrate；
（3）, in step（2）It is added distilled water in obtained concentrate, the mass ratio of concentrate and distilled water is 1：6, it is small to decoct 3
When, decoction liquor is centrifuged with 3500rpm, obtains supernatant；
（4）, by step（3）Obtained supernatant enters progress micro-filtration in microfiltration membranes and obtains purslane extract, and micro-filtration filter membrane is 19
Channel microfiltration membranes, internal diameter 8mm, outer diameter 12mm, pipe range 1000mm, 0.2 μm of film average pore size, membrane material Al2O3, membrane area
0.4m2, operation pressure difference control is in 0.01-0.2MPa.
Embodiment 8：
The present invention provides a kind of preparation methods of purslane extract, it includes the following steps：
（1）, prepare raw material purslane herb, impurity elimination, cleaning, 60 mesh are crushed to after drying, obtain purslane powder；
（2）, weigh purslane powder, into purslane powder be added 10 times amount volume fractions be 75% ethanol solution, in temperature
Be at 55 DEG C 30 minutes extraction times extract, obtain extracting solution, extracting solution is concentrated under reduced pressure into no alcohol taste and obtains concentrate；
（3）, in step（2）It is added distilled water in obtained concentrate, the mass ratio of concentrate and distilled water is 1：8, it is small to decoct 3
When, decoction liquor is centrifuged with 3300rpm, obtains supernatant；
（4）, by step（3）Obtained supernatant enters progress micro-filtration in microfiltration membranes and obtains purslane extract, and micro-filtration filter membrane is 19
Channel microfiltration membranes, internal diameter 8mm, outer diameter 12mm, pipe range 1000mm, 0.2 μm of film average pore size, membrane material Al2O3, membrane area
0.4m2, operation pressure difference control is in 0.01-0.2MPa.
Embodiment 9：
The present invention provides a kind of preparation methods of purslane extract, it includes the following steps：
（1）, prepare raw material purslane herb, impurity elimination, cleaning, 60 mesh are crushed to after drying, obtain purslane powder；
（2）, weigh purslane powder, into purslane powder be added 9 times amount volume fractions be 85% ethanol solution, in temperature
Be at 60 DEG C 35 minutes extraction times extract, obtain extracting solution, extracting solution is concentrated under reduced pressure into no alcohol taste and obtains concentrate；
（3）, in step（2）It is added distilled water in obtained concentrate, the mass ratio of concentrate and distilled water is 1：7, it is small to decoct 2
When, decoction liquor is centrifuged with 3200rpm, obtains supernatant；
（4）, by step（3）Obtained supernatant enters progress micro-filtration in microfiltration membranes and obtains purslane extract, and micro-filtration filter membrane is 19
Channel microfiltration membranes, internal diameter 8mm, outer diameter 12mm, pipe range 1000mm, 0.2 μm of film average pore size, membrane material Al2O3, membrane area
0.4m2, operation pressure difference control is in 0.01-0.2MPa.
Embodiment 10：
The present invention provides a kind of preparation methods of purslane extract, it includes the following steps：
（1）, prepare raw material purslane herb, impurity elimination, cleaning, 40 mesh are crushed to after drying, obtain purslane powder；
（2）, weigh purslane powder, into purslane powder be added 9 times amount volume fractions be 75% ethanol solution, in temperature
Be at 60 DEG C 30 minutes extraction times extract, obtain extracting solution, extracting solution is concentrated under reduced pressure into no alcohol taste and obtains concentrate；
（3）, in step（2）It is added distilled water in obtained concentrate, the mass ratio of concentrate and distilled water is 1：5, it is small to decoct 2
When, decoction liquor is centrifuged with 3000rpm, obtains supernatant；
（4）, by step（3）Obtained supernatant enters progress micro-filtration in microfiltration membranes and obtains purslane extract, and micro-filtration filter membrane is 19
Channel microfiltration membranes, internal diameter 8mm, outer diameter 12mm, pipe range 1000mm, 0.2 μm of film average pore size, membrane material Al2O3, membrane area
0.4m2, operation pressure difference control is in 0.01-0.2MPa.
What has been described above is only a preferred embodiment of the present invention, it is noted that for those skilled in the art,
Under the premise of not departing from general idea of the present invention, several changes and improvements can also be made, while the product is from dairy products angle
Belong to modulation breast series, is one kind of the newborn milk powder of modulation, these should also be considered as protection scope of the present invention.
Claims (4)
1. a kind of preparation method of purslane extract, it is characterised in that it includes the following steps：
（1）, prepare raw material purslane herb, impurity elimination, cleaning, 40-60 mesh is crushed to after drying, obtains purslane powder；
（2）, weigh purslane powder, it is 70%-95% ethanol solutions that 8-10 times of volume fraction measured is added into purslane powder,
In temperature be 45-60 DEG C at extraction time 20-40 minute extract, acquisition extracting solution, extracting solution is concentrated under reduced pressure into no alcohol
Taste obtains concentrate；
（3）, in step（2）It is added distilled water in obtained concentrate, the mass ratio of concentrate and distilled water is 1：（4-8）, decoct
It boils 2-3 hours, decoction liquor is centrifuged with 3000-3500rpm, obtains supernatant；
（4）, by step（3）Obtained supernatant enters progress micro-filtration in microfiltration membranes and obtains purslane extract.
2. the preparation method of purslane extract according to claim 1, it is characterised in that step（2）In, to purslane
In powder be added 8 times amount volume fractions be 75% ethanol solution, in temperature be 50 DEG C at 30 minutes extraction times extract,
Extracting solution is obtained, extracting solution is concentrated under reduced pressure into no alcohol taste and obtains concentrate.
3. the preparation method of purslane extract according to claim 1, it is characterised in that step（3）In, in step（2）
It is added distilled water in obtained concentrate, the mass ratio of concentrate and distilled water is 1：6.
4. the preparation method of purslane extract according to claim 1, it is characterised in that：Step（4）Middle micro-filtration filter membrane
For 19 channel microfiltration membranes, internal diameter 8mm, outer diameter 12mm, pipe range 1000mm, 0.2 μm of film average pore size, membrane material Al2O3, membrane area
0.4m2, operation pressure difference control is in 0.01-0.2MPa.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110025532A (en) * | 2019-04-28 | 2019-07-19 | 吴银 | A kind of sunscreen spray and preparation method thereof that stability is high |
| CN110251540A (en) * | 2019-07-23 | 2019-09-20 | 云南贝泰妮生物科技集团股份有限公司 | A kind of purslane low-temperature extraction method |
| CN112472728A (en) * | 2019-10-16 | 2021-03-12 | 广东丸美生物技术股份有限公司 | Purslane extract and preparation method thereof |
| CN112568431A (en) * | 2020-12-03 | 2021-03-30 | 山东腾贵医药有限公司 | Pre-meal dusting composition for inhibiting starch absorption and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101181317A (en) * | 2007-12-04 | 2008-05-21 | 中国人民解放军第二军医大学 | Purslane extract as well as preparation method and application thereof |
| CN101401830A (en) * | 2008-11-14 | 2009-04-08 | 武汉大学 | Preparation method for extracting flavone from purslane and uses thereof |
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2018
- 2018-08-27 CN CN201810982839.8A patent/CN108785341A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101181317A (en) * | 2007-12-04 | 2008-05-21 | 中国人民解放军第二军医大学 | Purslane extract as well as preparation method and application thereof |
| CN101401830A (en) * | 2008-11-14 | 2009-04-08 | 武汉大学 | Preparation method for extracting flavone from purslane and uses thereof |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110025532A (en) * | 2019-04-28 | 2019-07-19 | 吴银 | A kind of sunscreen spray and preparation method thereof that stability is high |
| CN110251540A (en) * | 2019-07-23 | 2019-09-20 | 云南贝泰妮生物科技集团股份有限公司 | A kind of purslane low-temperature extraction method |
| CN112472728A (en) * | 2019-10-16 | 2021-03-12 | 广东丸美生物技术股份有限公司 | Purslane extract and preparation method thereof |
| CN112568431A (en) * | 2020-12-03 | 2021-03-30 | 山东腾贵医药有限公司 | Pre-meal dusting composition for inhibiting starch absorption and preparation method thereof |
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Application publication date: 20181113 |
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