CN108785113B - 具有美白功效的天然分子组合物及其应用 - Google Patents
具有美白功效的天然分子组合物及其应用 Download PDFInfo
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Abstract
本发明涉及一种具有美白功效的天然分子组合物及其应用。该具有美白功效的天然分子组合物的活性成分包括有姜黄素、隐丹参酮、山奈酚和异鼠李素，所述姜黄素、隐丹参酮、山奈酚和异鼠李素的摩尔比为18‑25：3‑10：25‑33：58‑68。本发明的天然分子组合物能显著抑制黑色素的生成，从而具有非常显著的美白效果，其效果远好于各天然分子单独使用的效果，且安全性好，大大减低了伤害使用者皮肤的风险。
Description
技术领域
本发明涉及化妆品领域，特别是涉及一种具有美白功效的天然分子组合物及其应用。
背景技术
亚洲女性对于美白有执着的追求，于是市面上推出各种的美白产品。但有很多美白产品都被检验出其类固醇及重金属剂量超出标准，或者有些化学合成类的美白产品具有比较多的副作用，对人们的身体健康造成危害。因此，人们都希望有更天然，更安全和更有效的美白产品。
姜黄素是一种从姜黄根茎中提取得到的黄色色素。在日常生活中，姜黄素是一种天然色素，主要用于罐头、肠类制品、酱卤制品的染色，印度咖喱食品中的黄色就是使用姜黄素的经典例子。此外，姜黄素是一种很强的抗氧化剂，其分子式结构比较特殊，可选择性抑制蛋白酪氨酸激酶和血小板来源的生长因子受体，从而抑制细胞无序增殖，并使凋亡恢复，达到抗肿瘤目的。另外， 姜黄素具有降血脂、抗炎、利胆等作用。
隐丹参酮是从唇形科植物丹参的干燥根及根茎中提取的一种二萜醌类，橙色针状结晶化合物。据《神农本草经》记载，该典将丹参列为上品药材，意思就是没有毒性。另外在《日华子》内有记载“养神定志，通利关脉。治冷热劳，骨节疼痛，四肢不遂；排脓止痛，生肌长肉，破宿血，补新生血，安生胎，落死胎，止血崩带下；调妇人经脉不匀，血邪心烦；恶疮疥癣，瘿赘肿毒，丹毒，头痛，赤眼；热温狂闷”。可见丹参具有心肌保护，降血脂，抗胃溃疡改善循环的作用。是古代百姓日常生活的必备良药。在现代的研究上，丹参酮对金黄色葡萄球菌、耐甲氧西林金黄色葡萄球菌和β-内酰胺酶阳性的金黄色葡萄球菌具有明显的抑制作用，隐丹参酮破坏细菌细胞壁和细胞膜的结构，导致细胞膜通透性增加，进而使细胞内容物外泄；同时隐丹参酮对细菌蛋白质的合成有一定影响，使菌体内蛋白质减少，影响和阻碍细胞内蛋白质的表达，最终导致细菌正常生理功能的丧失。
山奈酚是一种天然黄酮类化合物，是存在于食用植物及传统药物中的黄色结晶有机化合物。在日常生活的蔬果中，例如生菜，菜心和苹果等均有一定含量的山柰酚。山奈酚对黄色葡萄球菌及绿脓杆菌、伤寒杆菌、痢疾杆菌均有抑制作用，可用于止咳，治疗支气管炎。山奈酚可抑制眼醛糖还原酶的活性，有利于糖尿病白内障的治疗。山奈酚还具有诱变剂活性，主要用于抗癌、抑制生育、抗癫痫、抗炎、抗氧化剂、解痉、抗溃疡、利胆利尿剂、止咳。
异鼠李素是另一种天然黄酮类化合物, 可以在许多天然植物类食品中发现这种植物营养素，尤其是在杏子，莳萝，茴香叶，红洋葱和萝卜叶子中的含量最丰富。异鼠李素和其他类黄酮一样，也是一种有助于保护身体免受自由基损害的强大抗氧化剂。它有预防多种类型癌症的功效，并可以减少与糖尿病有关的并发症。除此之外，异鼠李素还通过防止动脉硬化，高血压和心脏细胞氧化损坏保持心脏健康。
发明内容
基于此，本发明提供了一种具有美白功效的天然分子组合物。
具体技术方案如下：
一种具有美白功效的天然分子组合物，其活性成分包括有姜黄素、隐丹参酮、山奈酚和异鼠李素，所述姜黄素、隐丹参酮、山奈酚和异鼠李素的摩尔比为18 - 25：3 - 10 ：25- 33：58 - 68。
在其中一些实施例中，所述姜黄素、隐丹参酮、山奈酚和异鼠李素的摩尔比为20 -23：5 - 8 ：28 - 31：61 - 64。
在其中一些实施例中，所述姜黄素、隐丹参酮、山奈酚和异鼠李素的摩尔比为21 -22：6 - 7 ：29.5 - 30.5：61.5 - 62.5。
在其中一些实施例中，所述姜黄素、隐丹参酮、山奈酚和异鼠李素的摩尔比为21.54：6.48：29.90：62.00。
本发明还提供了上述天然分子组合物在制备美白产品中的应用。
在其中一些实施例中，所述美白产品为具有美白功效的化妆品、药品或食品。所述食品可以是饮料、食品添加剂或营养强化剂。
本发明还提供了一种具有美白功效的化妆品。
具体技术方案如下：
一种具有美白功效的化妆品，所述化妆品中的功效成分包括有上述具有美白功效的天然分子组合物。
本发明还提供了一种具有美白功效的药品或食品。
具体技术方案如下：
一种具有美白功效的药品或食品，所述药品或食品中的功效成分包括有上述具有美白功效的天然分子组合物。
本发明还提供了一种抑制黑色素生成的方法。
具体技术方案如下：
一种抑制黑色素生成的方法，包括以下步骤：将上述的具有美白功效的天然分子组合物配制成溶液，得到天然分子组合物溶液，使用所述天然分子组合物溶液抑制黑色素的生成，使用时将所述天然分子组合物溶液稀释8-12倍；所述姜黄素、隐丹参酮、山奈酚和异鼠李素在所述天然分子组合物溶液中的浓度分别为18 - 25µM、3 - 10 µM、25 - 33µM、58 - 68µM。
在其中一些实施例中，所述姜黄素、隐丹参酮、山奈酚和异鼠李素在所述天然分子组合物溶液中的浓度分别为20 - 23µM、5 - 8 µM、28 - 31µM、61 - 64µM。
在其中一些实施例中，所述姜黄素、隐丹参酮、山奈酚和异鼠李素在所述天然分子组合物溶液中的浓度分别为21 - 22µM、6 - 7 µM、29.5- 30.5µM、61.5 - 62.5µM。
在其中一些实施例中，所述姜黄素、隐丹参酮、山奈酚和异鼠李素在所述天然分子组合物溶液中的浓度分别为21.54µM、6.48µM、29.90µM、62.00µM。
本发明的具有美白功效的天然分子组合物具有以下优点和有益效果：
本发明的发明人经过大量实验研究发现：将姜黄素、隐丹参酮、山奈酚和异鼠李素以一定比例复配所得到的天然分子组合物能显著抑制黑色素的生成，从而具有非常显著的美白效果，其效果远好于各天然分子单独使用的效果。并通过进一步筛选得到了最适合的使用剂量（即天然分子组合物溶液中各分子的浓度），在本发明的剂量范围内使用时抑制黑色素生成的效果最为显著。
与一般美白配方相比，本发明的天然分子组合物中包含的活性分子均源自日常生活中经常接触的食品，由于采用纯天然分子，安全性好，大大减低了伤害使用者皮肤的风险。本发明的天然分子组合物可以广泛地用于药品、保健品、食品和美容产品，具有巨大的市场潜力。
附图说明
图1为实施例1中的不同剂量 (0.1µM - 100µM) 的姜黄素、隐丹参酮、山奈酚、异鼠李素和豆蔻明对B16-F10细胞的黑色素生成的抑制率结果；
图2为实施例1中的根据不同剂量 (0.1µM - 100µM) 的姜黄素、隐丹参酮、山奈酚、异鼠李素和豆蔻明对黑色素生成的抑制率结果通过FSC搜算法拟订出来的二次元方程式曲线图；
图3为实施例2中的100个组合配方对B16-F10细胞的黑色素生成抑制作用的实验结果。
具体实施方式
以下结合具体实施例对本发明的天然分子组合物做进一步详细的说明。
本发明的天然分子是指没有经过人工合成的，天然存在于动物、植物和微生物内的有机化合物。
实施例1
将天然分子姜黄素、隐丹参酮、山奈酚、异鼠李素和豆蔻明用二甲基亚砜溶解并稀释至100 mM，分别得到姜黄素的二甲基亚砜溶液、隐丹参酮的二甲基亚砜溶液、山奈酚的二甲基亚砜溶液、异鼠李素的二甲基亚砜溶液和豆蔻明的二甲基亚砜溶液；
分别把姜黄素、隐丹参酮、山奈酚、异鼠李素和豆蔻明的二甲基亚砜溶液用纯净水稀释成1µM、10µM、100µM、1mM的水溶性试剂。
将上述水溶性试剂进行黑色素生成抑制实验：
把B16-F10细胞 (CRL-6323™; American Type Culture Collection) 按每孔20,000枚的密度接种在96孔版上。培养基为加入10% FBS的DMEM，在接种B16-F10细胞的96孔版上每孔加入90µL的培养基，再每孔分别加入10µL上述不同浓度（剂量）的水溶性试剂，最后的终浓度分别为0.1µM、1µM、10µM、100µM，在37oC培养箱中培养2–4 天。空白对照组加入相同体积和比例的二甲基亚砜和水的混合溶液；阳性对照组加入相同体积的5 mM 的维生素C 及 2%的曲酸。
培养结束后，利用酶标仪在405nm的波长下测量培养液的吸光度，并计算对黑色素生成的抑制率。结果如图1所示，姜黄素、隐丹参酮、山奈酚、异鼠李素和豆蔻明在最佳剂量时对黑色素生成的抑制率分别为34.95%、70.92%、56.17%、51.64%、33.08%。将图1结果利用FSC搜算法（反馈系统控制搜算法(Feedback System Control)）拟订二次元方程式并计算虚拟最佳剂量，如图2和表1所示。
表1 经FSC搜算法计算出来的最佳剂量。
实施例2
将天然分子姜黄素、隐丹参酮、山奈酚、异鼠李素和豆蔻明用二甲基亚砜溶解并稀释至100 mM，分别得到姜黄素的二甲基亚砜溶液、隐丹参酮的二甲基亚砜溶液、山奈酚的二甲基亚砜溶液、异鼠李素的二甲基亚砜溶液和豆蔻明的二甲基亚砜溶液；
根据表2及表3中所述的剂量和比例把不同剂量比例的姜黄素、隐丹参酮、山奈酚、异鼠李素和豆蔻明的二甲基亚砜溶液混和，并用纯净水稀释制成1 - 100号水溶性测试配方。(例如23号水溶性测试配方为21.54 µM (5)的姜黄素、6.48 µM (4) 的隐丹参酮、29.90µM (6) 的山奈酚和62.00 µM (7)的异鼠李素)。
将上述水溶性测试配方进行黑色素生成抑制实验：
把B16-F10细胞 (CRL-6323™; American Type Culture Collection) 按每孔20,000枚的密度接种在96孔版上。培养基为加入10% FBS的DMEM，在接种B16-F10细胞的96孔版上每孔加入90µL的培养基，再每孔分别加入10µL上述不同浓度（剂量）的水溶性测试配方，在37oC培养箱中培养2–4 天。空白对照组加入相同体积和比例的二甲基亚砜和水的混合溶液；阳性对照组加入相同体积的5 mM 的维生素C 及 2%的曲酸。
培养结束后，利用酶标仪在405nm的波长下测量培养液的吸光度，并计算对黑色素生成的抑制率。结果如表4-表5和图3所示。其中，第1、11、23、28、49和91号水溶性测试配方对黑色素生成的抑制效果较好，抑制率均高于80%，23号配方的效果最好，抑制率大于87%。抑制率高于80%的其它配方中均含有豆蔻明，豆蔻明具有一定的毒性，安全性不够高，因此选择23号配方为最佳配方。
表2
表3
表4 不同剂量的水溶性测试配方的测试结果
表5 黑色素生成抑制率超过80.00%的配方
以上所述实施例的各技术特征可以进行任意的组合，为使描述简洁，未对上述实施例中的各个技术特征所有可能的组合都进行描述，然而，只要这些技术特征的组合不存在矛盾，都应当认为是本说明书记载的范围。
以上所述实施例仅表达了本发明的几种实施方式，其描述较为具体和详细，但并不能因此而理解为对发明专利范围的限制。应当指出的是，对于本领域的普通技术人员来说，在不脱离本发明构思的前提下，还可以做出若干变形和改进，这些都属于本发明的保护范围。因此，本发明专利的保护范围应以所附权利要求为准。
Claims (6)
1.一种具有美白功效的天然分子组合物，其特征在于，由姜黄素、隐丹参酮、山奈酚和异鼠李素组成，所述姜黄素、隐丹参酮、山奈酚和异鼠李素的摩尔比为18 - 25：3 - 10 ：25- 33：58 - 68。
2.根据权利要求1所述的具有美白功效的天然分子组合物，其特征在于，所述姜黄素、隐丹参酮、山奈酚和异鼠李素的摩尔比为20 - 23：5 - 8 ：28 - 31：61 - 64。
3.根据权利要求2所述的具有美白功效的天然分子组合物，其特征在于，所述姜黄素、隐丹参酮、山奈酚和异鼠李素的摩尔比为21.54：6.48：29.90：62.00。
4.权利要求1-3任一项所述的天然分子组合物在制备美白产品中的应用。
5.根据权利要求4所述的应用，其特征在于，所述美白产品为具有美白功效的化妆品、药品或食品。
6.一种具有美白功效的化妆品、药品或食品，其特征在于，所述化妆品、药品或食品中的功效成分包括有权利要求1-3任一项所述的具有美白功效的天然分子组合物。
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101411745A (zh) * | 2007-10-15 | 2009-04-22 | 博仲盛景医药技术（北京）有限公司 | 一种抗菌丹参酮提取物、其制备方法、用途及其产品 |
| CN102198066A (zh) * | 2011-05-31 | 2011-09-28 | 广州保税区雅兰国际化妆品有限公司 | 一种应用于化妆品的新型美白祛斑剂 |
| CN104721064A (zh) * | 2013-12-23 | 2015-06-24 | 天津芸熙生物技术有限公司 | 一种美白皮肤的组合物及化妆品 |
| CN104958257A (zh) * | 2015-06-16 | 2015-10-07 | 南方医科大学 | 一种隐丹参酮皮肤角质类脂体制剂及其制备方法 |
| CN105963637A (zh) * | 2016-06-15 | 2016-09-28 | 中山大学 | 隐丹参酮联合姜黄素在制备肿瘤治疗药物中的应用 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101411745A (zh) * | 2007-10-15 | 2009-04-22 | 博仲盛景医药技术（北京）有限公司 | 一种抗菌丹参酮提取物、其制备方法、用途及其产品 |
| CN102198066A (zh) * | 2011-05-31 | 2011-09-28 | 广州保税区雅兰国际化妆品有限公司 | 一种应用于化妆品的新型美白祛斑剂 |
| CN104721064A (zh) * | 2013-12-23 | 2015-06-24 | 天津芸熙生物技术有限公司 | 一种美白皮肤的组合物及化妆品 |
| CN104958257A (zh) * | 2015-06-16 | 2015-10-07 | 南方医科大学 | 一种隐丹参酮皮肤角质类脂体制剂及其制备方法 |
| CN105963637A (zh) * | 2016-06-15 | 2016-09-28 | 中山大学 | 隐丹参酮联合姜黄素在制备肿瘤治疗药物中的应用 |
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