CN108783124A - A kind of production method rich in oligofructose juice beverage - Google Patents
A kind of production method rich in oligofructose juice beverage Download PDFInfo
- Publication number
- CN108783124A CN108783124A CN201810463895.0A CN201810463895A CN108783124A CN 108783124 A CN108783124 A CN 108783124A CN 201810463895 A CN201810463895 A CN 201810463895A CN 108783124 A CN108783124 A CN 108783124A
- Authority
- CN
- China
- Prior art keywords
- oligofructose
- juice beverage
- production method
- transfructosylase
- citric acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 235000011389 fruit/vegetable juice Nutrition 0.000 title claims abstract description 56
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 22
- 102000004190 Enzymes Human genes 0.000 claims abstract description 34
- 108090000790 Enzymes Proteins 0.000 claims abstract description 34
- 240000000111 Saccharum officinarum Species 0.000 claims abstract description 34
- 235000007201 Saccharum officinarum Nutrition 0.000 claims abstract description 34
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 23
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Natural products OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 53
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 claims description 39
- 239000000243 solution Substances 0.000 claims description 33
- 229910052593 corundum Inorganic materials 0.000 claims description 22
- 229910001845 yogo sapphire Inorganic materials 0.000 claims description 22
- 239000000203 mixture Substances 0.000 claims description 21
- 239000007853 buffer solution Substances 0.000 claims description 18
- 239000006228 supernatant Substances 0.000 claims description 16
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 claims description 14
- 238000001035 drying Methods 0.000 claims description 12
- 239000000872 buffer Substances 0.000 claims description 11
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 10
- 239000012530 fluid Substances 0.000 claims description 9
- 230000002779 inactivation Effects 0.000 claims description 9
- XPFJYKARVSSRHE-UHFFFAOYSA-K trisodium;2-hydroxypropane-1,2,3-tricarboxylate;2-hydroxypropane-1,2,3-tricarboxylic acid Chemical compound [Na+].[Na+].[Na+].OC(=O)CC(O)(C(O)=O)CC(O)=O.[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O XPFJYKARVSSRHE-UHFFFAOYSA-K 0.000 claims description 9
- 238000009835 boiling Methods 0.000 claims description 8
- 229910021645 metal ion Inorganic materials 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 8
- 239000002245 particle Substances 0.000 claims description 8
- 239000007787 solid Substances 0.000 claims description 8
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 7
- 239000012153 distilled water Substances 0.000 claims description 7
- 229910052708 sodium Inorganic materials 0.000 claims description 7
- 239000011734 sodium Substances 0.000 claims description 7
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 6
- 229910001960 metal nitrate Inorganic materials 0.000 claims description 6
- 229910052742 iron Inorganic materials 0.000 claims description 5
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 4
- 238000005119 centrifugation Methods 0.000 claims description 4
- 238000001816 cooling Methods 0.000 claims description 4
- 239000011701 zinc Substances 0.000 claims description 4
- 229910052725 zinc Inorganic materials 0.000 claims description 4
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 3
- 239000000589 Siderophore Substances 0.000 claims description 3
- BRPQOXSCLDDYGP-UHFFFAOYSA-N calcium oxide Chemical compound [O-2].[Ca+2] BRPQOXSCLDDYGP-UHFFFAOYSA-N 0.000 claims description 3
- 239000000292 calcium oxide Substances 0.000 claims description 3
- ODINCKMPIJJUCX-UHFFFAOYSA-N calcium oxide Inorganic materials [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 claims description 3
- 229910052802 copper Inorganic materials 0.000 claims description 3
- 239000010949 copper Substances 0.000 claims description 3
- 229910052751 metal Inorganic materials 0.000 claims description 3
- 239000002184 metal Substances 0.000 claims description 3
- 229910052759 nickel Inorganic materials 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 238000004064 recycling Methods 0.000 claims description 3
- 239000000377 silicon dioxide Substances 0.000 claims description 3
- 235000012239 silicon dioxide Nutrition 0.000 claims description 3
- 238000007598 dipping method Methods 0.000 claims description 2
- 238000001802 infusion Methods 0.000 claims description 2
- 230000008569 process Effects 0.000 claims description 2
- 238000010532 solid phase synthesis reaction Methods 0.000 claims description 2
- 239000011534 wash buffer Substances 0.000 claims description 2
- PXRKCOCTEMYUEG-UHFFFAOYSA-N 5-aminoisoindole-1,3-dione Chemical compound NC1=CC=C2C(=O)NC(=O)C2=C1 PXRKCOCTEMYUEG-UHFFFAOYSA-N 0.000 claims 1
- 240000007594 Oryza sativa Species 0.000 claims 1
- 235000007164 Oryza sativa Nutrition 0.000 claims 1
- 235000009566 rice Nutrition 0.000 claims 1
- 229930006000 Sucrose Natural products 0.000 abstract description 11
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 abstract description 11
- 239000005720 sucrose Substances 0.000 abstract description 10
- 230000000694 effects Effects 0.000 abstract description 8
- 230000036541 health Effects 0.000 abstract description 5
- 238000012423 maintenance Methods 0.000 abstract description 5
- 235000000346 sugar Nutrition 0.000 abstract description 5
- 239000000758 substrate Substances 0.000 abstract description 4
- 102000051366 Glycosyltransferases Human genes 0.000 abstract 1
- 108700023372 Glycosyltransferases Proteins 0.000 abstract 1
- 235000005979 Citrus limon Nutrition 0.000 description 17
- 244000131522 Citrus pyriformis Species 0.000 description 17
- 239000001509 sodium citrate Substances 0.000 description 10
- 229960004793 sucrose Drugs 0.000 description 10
- 238000006243 chemical reaction Methods 0.000 description 6
- 239000002253 acid Substances 0.000 description 5
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 5
- 238000003756 stirring Methods 0.000 description 5
- 235000013399 edible fruits Nutrition 0.000 description 4
- 238000004128 high performance liquid chromatography Methods 0.000 description 3
- 229910001453 nickel ion Inorganic materials 0.000 description 3
- KBJMLQFLOWQJNF-UHFFFAOYSA-N nickel(ii) nitrate Chemical compound [Ni+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O KBJMLQFLOWQJNF-UHFFFAOYSA-N 0.000 description 3
- 238000002604 ultrasonography Methods 0.000 description 3
- 229930091371 Fructose Natural products 0.000 description 2
- 239000005715 Fructose Substances 0.000 description 2
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 2
- 239000004411 aluminium Substances 0.000 description 2
- 229910052782 aluminium Inorganic materials 0.000 description 2
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 2
- 235000001014 amino acid Nutrition 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000000969 carrier Substances 0.000 description 2
- FTSSQIKWUOOEGC-RULYVFMPSA-N fructooligosaccharide Chemical compound OC[C@H]1O[C@@](CO)(OC[C@@]2(OC[C@@]3(OC[C@@]4(OC[C@@]5(OC[C@@]6(OC[C@@]7(OC[C@@]8(OC[C@@]9(OC[C@@]%10(OC[C@@]%11(O[C@H]%12O[C@H](CO)[C@@H](O)[C@H](O)[C@H]%12O)O[C@H](CO)[C@@H](O)[C@@H]%11O)O[C@H](CO)[C@@H](O)[C@@H]%10O)O[C@H](CO)[C@@H](O)[C@@H]9O)O[C@H](CO)[C@@H](O)[C@@H]8O)O[C@H](CO)[C@@H](O)[C@@H]7O)O[C@H](CO)[C@@H](O)[C@@H]6O)O[C@H](CO)[C@@H](O)[C@@H]5O)O[C@H](CO)[C@@H](O)[C@@H]4O)O[C@H](CO)[C@@H](O)[C@@H]3O)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@@H](O)[C@@H]1O FTSSQIKWUOOEGC-RULYVFMPSA-N 0.000 description 2
- 229940107187 fructooligosaccharide Drugs 0.000 description 2
- 235000019605 sweet taste sensations Nutrition 0.000 description 2
- ONDPHDOFVYQSGI-UHFFFAOYSA-N zinc nitrate Chemical compound [Zn+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ONDPHDOFVYQSGI-UHFFFAOYSA-N 0.000 description 2
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 1
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 description 1
- 208000007848 Alcoholism Diseases 0.000 description 1
- 241000186000 Bifidobacterium Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- 108010042889 Inulosucrase Proteins 0.000 description 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- 208000008589 Obesity Diseases 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 201000007930 alcohol dependence Diseases 0.000 description 1
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 description 1
- 230000000675 anti-caries Effects 0.000 description 1
- 235000003704 aspartic acid Nutrition 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000003599 food sweetener Nutrition 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000005802 health problem Effects 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- MVFCKEFYUDZOCX-UHFFFAOYSA-N iron(2+);dinitrate Chemical compound [Fe+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O MVFCKEFYUDZOCX-UHFFFAOYSA-N 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 239000001630 malic acid Substances 0.000 description 1
- 235000011090 malic acid Nutrition 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000020824 obesity Nutrition 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 235000004400 serine Nutrition 0.000 description 1
- 238000010025 steaming Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000003765 sweetening agent Substances 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
- A23L2/60—Sweeteners
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
- A23L2/84—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Polymers & Plastics (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Mycology (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Non-Alcoholic Beverages (AREA)
Abstract
The invention discloses a kind of production methods rich in oligofructose juice beverage, glycosyl transferase is immobilized on nano-oxide carrier not soluble in water, so that the enzyme of immobilization is easily detached with substrate, product, then use the transfructosylase of the immobilization by the sucrose inversion in sugar-cane juice for oligofructose, reduce sugar-cane juice sugar, the health maintenance effect for improving sugarcane juice improves the value of sugar-cane juice.
Description
Technical field
The present invention relates to technical field of beverage preparation, and in particular to a kind of producer rich in oligofructose juice beverage
Method.
Background technology
Sugar-cane juice contains the essential trace elements of the human bodys such as a large amount of iron, zinc, manganese, and the content of wherein iron is especially more, up to 9
Mg/kg is occupied first of fruit, is known as the laudatory title of " fruit of enriching blood ".In addition, sugar-cane juice also contains glutamic acid, aspartic acid, serine
Etc. a variety of amino acid, and contain the organic acids such as malic acid, citric acid.The fresh juice of sugarcane has full of nutrition, delicious flavour, defaecation solution
Knot alleviates the features such as alcoholism, has heat-clearing, promotes the production of body fluid, lower gas, moisturizes, tonifying lung benefit stomach and other effects, deep to be liked by consumers in general
Love.But contain a large amount of sucrose, fructose and glucose, wherein cane sugar content 12%-18% in sugarcane juice, a large amount of of these sugars take the photograph
Enter, is easy to cause some health problems, such as the problems such as decayed tooth, obesity, therefore people's is not only to have liked but also be afraid of to sugarcane juice.
Oligofructose is a kind of natural active matter, and sugariness is 0.3-0.6 times of sucrose, the pure sweet taste of existing sucrose,
Again salubriouser than sucrose sweet taste, being one kind having adjusting intestinal flora, is proliferated Bifidobacterium, promotes the absorption of calcium, adjusts blood fat, exempt from
Epidemic disease is adjusted, the novel sweetener of the functions such as anti-caries tooth.It, on the one hand can be with if can be oligofructose by the sucrose inversion in sugarcane juice
The effect of reducing the sugar content of sugarcane juice, while also improving sugarcane juice.
Transfructosylase energy catalysing sucrose is converted into fructooligosaccharide, but the enzyme is soluble easily in water, expensive, after production
Difficulty is detached from substrate, can only be utilized once, be caused production cost height.
Invention content
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of productions rich in oligofructose juice beverage
Sucrose inversion in sugar-cane juice is that the sugarcane fruit with health maintenance effect is low using the transfructosylase of immobilization by method, this method
Glycan reduces sugar-cane juice sugar, improves the health maintenance effect of sugarcane juice, improves the value of sugar-cane juice.
To achieve the above object, the present invention adopts the following technical scheme that：
A kind of production method rich in oligofructose juice beverage, comprises the following steps：
A, the metal-nitrate solutions of wt%=1-20% are impregnated into nano-oxide carrier, stand 18-24h, drying, 480-
500 DEG C of roastings, obtain the nano-oxide carrier of metal ion load；
B, transfructosylase is dissolved in the citric acid-sodium citrate buffer of pH=6-8, is configured to concentration 10mg/mL
Enzyme solution, by every 10-90mg metal ions load nano-oxide carrier be added 10mL water in, ultrasonic 15-25min, be added
0.1-0.9mL enzyme solutions are put into shaking table and react 20-24h in 28-32 DEG C, cast out supernatant, and solid particle is with citric acid-lemon
Sour sodium wash buffer 2-3 times, obtains the transfructosylase of immobilization；
C, the transfructosylase of immobilization is added in triangular flask, the sugar-cane juice of 45-55mL is added, obtain mixture, used
It is 5.8-6.2 that citric acid-sodium citrate buffer, which adjusts mixture pH, by mixture in 45-55 DEG C, 180-220r/min perseverances
50-70min is reacted in warm Clothoid type shaking table, takes out mixture, inactivation, cooling, centrifugation, supernatant is to be rich in oligofructose sugarcane
Juice beverage, while recycling the transfructosylase of immobilization.
In step A, the metal in the metal-nitrate solutions includes the metal ions such as nickel, copper, zinc, iron.
In step A, the nano-oxide carrier includes nano oxidized alumina supporter, nanometer silicon dioxide, nano oxygen
Change other oxide carriers such as siderophore, nano calcium oxide carrier.
The preparation method of the nano oxidized alumina supporter is：It is Al by molar ratio：Citric acid=1:2 mixture is dissolved in steaming
In distilled water, viscous fluid is stirred to clarify, viscous fluid is heated into 4-4.5 h to evaporate excessive moisture in 75-80 DEG C of water-bath, then
10-14 h are heated under 85-90 DEG C of constant temperature, obtained gel are placed in, 110-130 DEG C of dry 10-14 h, then at
480-500 DEG C of roasting, obtains a nanometer Al2O3Carrier.
In step A, the method for the dipping includes equi-volume impregnating, excessive infusion process, solid-phase synthesis.
In step B, the pH of the citric acid-sodium citrate buffer solution is 5.5-6.0
In step C, the inactivation, cooling, centrifugation concrete operations are：It takes out mixture and is put into boiling water bath and boil 10min, it is cold
But to room temperature, 4000-5000r/min centrifuges 2-3min.
The present invention is immobilized on carrier not soluble in water by transfructosylase using above technical scheme so that immobilization
Enzyme easily detached with substrate, product, there is no the residual of enzyme in reaction mixture, purifying technique simplifies, and can realize successive reaction, be convenient for
Then automated production uses the transfructosylase of the immobilization by the sucrose inversion in sugar-cane juice for oligofructose.
The immobilized of transfructosylase is the key that the present invention and innovative point, and the present invention utilizes aluminium and the amino acid in enzyme
Chelating forms complex, and reaction condition is mild, it is not easy to influence the structure and activity of enzyme；Nickel ion is swashing for transfructosylase
Agent living, the two is combined together, and by the way that nickel ion to be entrained in ordered mesoporous aluminium oxide carrier, has both utilized swashing for nickel ion
Viability, and the active force of aluminium and enzyme coordinate bond is utilized, meanwhile, the specific surface area for making full use of aluminium oxide big is conducive to immobilization
The contact of enzyme-to-substrate, triplicity is got up, and has synthesized the immobilized fructosyltransferase of a kind of high activity, high stability,
It is the fructooligosaccharide with health maintenance effect by the sucrose inversion in sugar-cane juice, reduces sugar-cane juice sugar, improve sugarcane juice
Health maintenance effect improves the value of sugar-cane juice.
Specific implementation mode
A kind of production method rich in oligofructose juice beverage, comprises the following steps：
A, the metal-nitrate solutions of wt%=1-20% are impregnated into nano-oxide carrier, stand 18-24h, drying, 480-
500 DEG C of roastings, obtain the nano-oxide carrier of metal ion load；
B, transfructosylase is dissolved in the citric acid-sodium citrate buffer of pH=6-8, is configured to concentration 10mg/mL
Enzyme solution, by every 10-90mg metal ions load nano-oxide carrier be added 10mL water in, ultrasonic 15-25min, be added
0.1-0.9mL enzyme solutions are put into shaking table and react 20-24h in 28-32 DEG C, cast out supernatant, solid particle is 5.5-6.0 with pH
Citric acid-sodium citrate buffer solution is rinsed 2-3 times, and the transfructosylase of immobilization is obtained；
C, the transfructosylase of immobilization is added in triangular flask, the sugar-cane juice of 45-55mL is added, obtain mixture, used
It is 5.8-6.2 that citric acid-sodium citrate buffer, which adjusts mixture pH, by mixture in 45-55 DEG C, 180-220r/min perseverances
50-70min is reacted in warm Clothoid type shaking table, is taken out mixture and is put into boiling water bath and boil 10min, is cooled to room temperature, 4000-
5000r/min centrifuges 2-3min, and supernatant is to be rich in oligofructose juice beverage, while recycling the fructose group-transfer of immobilization
Enzyme.
In step A, the metal in the metal-nitrate solutions includes the metal ions such as nickel, copper, zinc, iron.
The nano-oxide carrier include nano oxidized alumina supporter, nanometer silicon dioxide, nano oxidized siderophore,
Other oxide carriers such as nano calcium oxide carrier.
In following embodiment by taking nano oxidized alumina supporter as an example.
Embodiment 1
A kind of production method rich in oligofructose juice beverage, comprises the following steps：
（1）It is Al by molar ratio：Citric acid=1:2 mixture is dissolved in a small amount of distilled water, stirs to clarify viscous fluid, by this
It is slowly heated 4h in 80 DEG C of solution water-bath, 12 h are heated under 90 DEG C of constant temperatures again to evaporate excessive moisture.By what is obtained
Gel is placed in 120 DEG C of 12 h of drying, and at 500 DEG C, roasting obtains a nanometer Al2O3, it is denoted as n-Al2O3。
（2）The nickel nitrate solution of wt%=1% is added to n-Al2O3In, it stands for 24 hours, drying, it is negative to obtain Ni for 500 DEG C of roastings
The nanometer Al of load2O3, it is denoted as n-Al2O3-1Ni。
（3）With the citric acid and sodium citrate buffer of pH=6, transfructosylase is dissolved in above-mentioned buffer solution
In, it is configured to the enzyme solution of 10mg/mL concentration, weighs 10 mg nanometers of n-Al2O310mL water, ultrasound is added in -1Ni alumina supports
0.1mL enzyme solutions are added in 20min, are put into 30 DEG C of reactions in shaking table and for 24 hours, go supernatant, the lemon that solid particle is 6.0 with pH
Acid-sodium citrate buffer solution rinses 2-3 times and obtains immobilised enzymes.
（4）The transfructosylase of immobilization is added in triangular flask, the sugar-cane juice citric acid and lemon of 50mL is added
Lemon acid sodium buffer solution is adjusted to pH=6 or so, at 50 DEG C, 1h is reacted in 200r/min constant temperature Clothoid type shaking tables, taking-up is put into boiling
10min inactivations are boiled in water-bath, are cooled to room temperature, and 5000r/min centrifuges 2min, takes its supernatant, is as rich in oligofructose
Juice beverage detects oligofructose content with HPLC, and oligofructose yield is 36%.
（5）The transfructosylase enzyme for the immobilization that above-mentioned steps centrifuge is recycled simultaneously, is repeated and is utilized.
Embodiment 2
A kind of production method rich in oligofructose juice beverage, comprises the following steps：
（1）It is Al by molar ratio：Citric acid=1:2 mixture is dissolved in a small amount of distilled water, stirs to clarify viscous fluid, by this
It is slowly heated 4h in 80 DEG C of solution water-bath, 12 h are heated under 90 DEG C of constant temperatures again to evaporate excessive moisture.By what is obtained
Gel is placed in 120 DEG C of 12 h of drying, and at 500 DEG C, roasting obtains a nanometer Al2O3, it is denoted as n-Al2O3。
（2）The nickel nitrate solution of wt%=5% is added to n-Al2O3In, it stands for 24 hours, drying, it is negative to obtain Ni for 500 DEG C of roastings
The nanometer Al of load2O3, it is denoted as n-Al2O3-5Ni。
（3）With the citric acid and sodium citrate buffer of pH=6, transfructosylase is dissolved in above-mentioned buffer solution
In, it is configured to the enzyme solution of 10mg/mL concentration, weighs 10 mg nanometers of n-Al2O310mL water, ultrasound is added in -5Ni alumina supports
0.1mL enzyme solutions are added in 20min, are put into 30 DEG C of reactions in shaking table and for 24 hours, go supernatant, the lemon that solid particle is 6.0 with pH
Acid-sodium citrate buffer solution rinses 2-3 times and obtains immobilised enzymes.
（4）The transfructosylase of immobilization is added in triangular flask, the sugar-cane juice citric acid and lemon of 50mL is added
Lemon acid sodium buffer solution is adjusted to pH=6 or so, at 50 DEG C, 1h is reacted in 200r/min constant temperature Clothoid type shaking tables, taking-up is put into boiling
10min inactivations are boiled in water-bath, are cooled to room temperature, and 5000r/min centrifuges 2min, takes its supernatant, is as rich in oligofructose
Juice beverage detects oligofructose content with HPLC.Oligofructose yield is 40%.
（5）The transfructosylase enzyme for the immobilization that above-mentioned steps centrifuge is recycled simultaneously, is repeated and is utilized.
Embodiment 3
A kind of production method rich in oligofructose juice beverage, comprises the following steps：
（1）It is Al by molar ratio：Citric acid=1:2 mixture is dissolved in a small amount of distilled water, stirs to clarify viscous fluid, by this
It is slowly heated 4h in 80 DEG C of solution water-bath, 12 h are heated under 90 DEG C of constant temperatures again to evaporate excessive moisture.By what is obtained
Gel is placed in 120 DEG C of 12 h of drying, and at 500 DEG C, roasting obtains a nanometer Al2O3, it is denoted as n-Al2O3。
（2）The nickel nitrate solution of wt%=1% is added to n-Al2O3In, it stands for 24 hours, drying, 500 DEG C of roastings obtain Ni
The nanometer Al of load2O3, it is denoted as n-Al2O3-1Ni。
（3）With the citric acid and sodium citrate buffer of pH=6, transfructosylase is dissolved in above-mentioned buffer solution
In, it is configured to the enzyme solution of 10mg/mL concentration, weighs 10 mg nanometers of n-Al2O310mL water, ultrasound is added in -1Ni alumina supports
0.9mL enzyme solutions are added in 20min, are put into 30 DEG C of reactions in shaking table and for 24 hours, go supernatant, the lemon that solid particle is 6.0 with pH
Acid-sodium citrate buffer solution rinses 2-3 times and obtains immobilised enzymes.
（4）The transfructosylase of immobilization is added in triangular flask, the sugar-cane juice citric acid and lemon of 50mL is added
Lemon acid sodium buffer solution is adjusted to pH=6 or so, at 50 DEG C, is reacted for 24 hours in 200r/min constant temperature Clothoid type shaking tables, taking-up is put into
10min inactivations are boiled in boiling water bath, are cooled to room temperature, and 5000r/min centrifuges 2min, takes its supernatant, is as rich in oligomeric fruit
Sugarcane juice beverage detects oligofructose content with HPLC.Oligofructose yield is 60%.
（5）The transfructosylase enzyme for the immobilization that above-mentioned steps centrifuge is recycled simultaneously, is repeated and is utilized.
Embodiment 4
A kind of production method rich in oligofructose juice beverage, comprises the following steps：
（1）It is Al by molar ratio：Citric acid=1:2 mixture is dissolved in a small amount of distilled water, stirs to clarify viscous fluid, by this
It is slowly heated 4.5h in 75 DEG C of solution water-bath, 14h is heated under 85 DEG C of constant temperatures again to evaporate excessive moisture.By what is obtained
Gel is placed in 110 DEG C of 14 h of drying, and at 480 DEG C, roasting obtains a nanometer Al2O3, it is denoted as n-Al2O3。
（2）The zinc nitrate solution of wt%=20% is added to n-Al2O3In, 18h, drying are stood, 480 DEG C of roastings obtain Ni
The nanometer Al of load2O3, it is denoted as n-Al2O3-20Zn。
（3）With the citric acid and sodium citrate buffer of pH=8, transfructosylase is dissolved in above-mentioned buffer solution
In, it is configured to the enzyme solution of 10mg/mL concentration, weighs 100 mg nanometers of n-Al2O310mL water is added in -20Zn alumina supports, surpasses
0.9mL enzyme solutions are added in sound 15min, are put into 32 DEG C of reaction 20h in shaking table, remove supernatant, the lemon that solid particle is 6.0 with pH
Lemon acid-sodium citrate buffer solution rinses 2 times and obtains immobilised enzymes.
（4）The transfructosylase of immobilization is added in triangular flask, the sugar-cane juice citric acid and lemon of 55mL is added
Lemon acid sodium buffer solution is adjusted to pH=5.8, at 55 DEG C, 1h is reacted in 180r/min constant temperature Clothoid type shaking tables, taking-up is put into boiling water
10min inactivations are boiled in bath, are cooled to room temperature, and 4000r/min centrifuges 3min, takes its supernatant, is as rich in oligofructose sugarcane
Juice beverage.
（5）The transfructosylase enzyme for the immobilization that above-mentioned steps centrifuge is recycled simultaneously, is repeated and is utilized.
Embodiment 5
A kind of production method rich in oligofructose juice beverage, comprises the following steps：
（1）It is Al by molar ratio：Citric acid=1:2 mixture is dissolved in a small amount of distilled water, stirs to clarify viscous fluid, by this
It is slowly heated 4h in 80 DEG C of solution water-bath, 10h is heated under 90 DEG C of constant temperatures again to evaporate excessive moisture.It is solidifying by what is obtained
Glue is placed in 130 DEG C of dry 10h, and at 480 DEG C, roasting obtains a nanometer Al2O3, it is denoted as n-Al2O3。
（2）The iron nitrate solution of wt%=10% is added to n-Al2O3In, it stands for 24 hours, drying, 500 DEG C of roastings obtain Ni
The nanometer Al of load2O3, it is denoted as n-Al2O3-10Fe。
（3）With the citric acid and sodium citrate buffer of pH=6, transfructosylase is dissolved in above-mentioned buffer solution
In, it is configured to the enzyme solution of 10mg/mL concentration, weighs 50 mg nanometers of n-Al2O310mL water is added in -10Fe 1Ni alumina supports,
Ultrasonic 20min is added 0.5mL enzyme solutions, is put into shaking table and answers 20h for 32 DEG C, removes supernatant, the lemon that solid particle is 6.0 with pH
Lemon acid-sodium citrate buffer solution rinses 2-3 times and obtains immobilised enzymes.
（4）The transfructosylase of immobilization is added in triangular flask, the sugar-cane juice citric acid and lemon of 45mL is added
Lemon acid sodium buffer solution is adjusted to pH=6.2, at 45 DEG C, 1.5h is reacted in 220r/min constant temperature Clothoid type shaking tables, taking-up is put into boiling
10min inactivations are boiled in water-bath, are cooled to room temperature, and 5000r/min centrifuges 2min, takes its supernatant, is as rich in oligofructose
Juice beverage.
（5）The transfructosylase enzyme for the immobilization that above-mentioned steps centrifuge is recycled simultaneously, is repeated and is utilized.
Claims (7)
1. a kind of production method rich in oligofructose juice beverage, it is characterised in that：It includes following steps：
A, the metal-nitrate solutions of wt%=1-20% are impregnated into nano-oxide carrier, stand 18-24h, drying, 480-
500 DEG C of roastings, obtain the nano-oxide carrier of metal ion load；
B, transfructosylase is dissolved in the citric acid-sodium citrate buffer of pH=6-8, is configured to concentration 10mg/mL
Enzyme solution, by every 10-90mg metal ions load nano-oxide carrier be added 10mL water in, ultrasonic 15-25min, be added
0.1-0.9mL enzyme solutions are put into shaking table and react 20-24h in 28-32 DEG C, cast out supernatant, and solid particle is with citric acid-lemon
Sour sodium wash buffer 2-3 times, obtains the transfructosylase of immobilization；
C, the transfructosylase of immobilization is added in triangular flask, the sugar-cane juice of 45-55mL is added, obtain mixture, used
It is 5.8-6.2 that citric acid-sodium citrate buffer, which adjusts mixture pH, by mixture in 45-55 DEG C, 180-220r/min perseverances
50-70min is reacted in warm Clothoid type shaking table, takes out mixture, inactivation, cooling, centrifugation, supernatant is to be rich in oligofructose sugarcane
Juice beverage, while recycling the transfructosylase of immobilization.
2. a kind of production method rich in oligofructose juice beverage according to claim 1, it is characterised in that：Step A
In, the metal in the metal-nitrate solutions includes one kind in nickel, copper, zinc or iron.
3. a kind of production method rich in oligofructose juice beverage according to claim 1, it is characterised in that：Step A
In, the nano-oxide carrier includes nano oxidized alumina supporter, nanometer silicon dioxide, nano oxidized siderophore, nanometer
One kind in calcium oxide carrier.
4. a kind of production method rich in oligofructose juice beverage according to claim 3, it is characterised in that：It is described to receive
Rice alumina support preparation method be：It is Al by molar ratio：Citric acid=1:2 mixture is dissolved in distilled water, is stirred to clear
Viscous fluid is heated 4-4.5 h to evaporate excessive moisture, then in 85-90 DEG C of constant temperature item by clear viscous fluid in 75-80 DEG C of water-bath
10-14 h are heated under part, obtained gel are placed in, 110-130 DEG C of dry 10-14 h, are roasted, are obtained then at 480-500 DEG C
Nanometer Al2O3Carrier.
5. a kind of production method rich in oligofructose juice beverage according to claim 1, it is characterised in that：Step
In A, the method for the dipping includes equi-volume impregnating, excessive infusion process, solid-phase synthesis.
6. a kind of production method rich in oligofructose juice beverage according to claim 1, it is characterised in that：Step B
In, the pH of the citric acid-sodium citrate buffer solution is 5.5-6.0.
7. a kind of production method rich in oligofructose juice beverage according to claim 1, it is characterised in that：Step C
In, the inactivation, cooling, centrifugation concrete operations are：It takes out mixture and is put into boiling water bath and boil 10min, be cooled to room temperature,
4000-5000r/min centrifuges 2-3min.
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