CN108753905A - A kind of application of porous carbon materials - Google Patents
A kind of application of porous carbon materials Download PDFInfo
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- CN108753905A CN108753905A CN201810681903.9A CN201810681903A CN108753905A CN 108753905 A CN108753905 A CN 108753905A CN 201810681903 A CN201810681903 A CN 201810681903A CN 108753905 A CN108753905 A CN 108753905A
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Abstract
Carbon is applied the invention discloses a kind of porous carbon materials.This porous carbon materials also has micropore, mesoporous and/or macropore, the cephalosporin analog antibiotic in adsorbable clinical sample by having many vesica shape macropores after rice husk carbonizing and sodium hydroxide activation heat treatment on the hole wall of the macropore.The porous carbon materials are applied to the detection of microorganism in blood culture, can significantly improve the positive rate of microorganism detection.
Description
Technical field
The present invention relates to the applications that a kind of porous carbon materials adsorb antibiotic in the biological sample.
Background technology
Antibiotic is the analog of certain such as bacteriums, actinomyces, the metabolite of fungi microorganism or synthesis, low dose of
When have the killing or inhibiting effect of strength to various pathogenic microorganisms.Antibiotic is removed for medical treatment, is also applied to bioscience and is ground
Study carefully, agricultural, animal husbandry, aquatic products and livestock and poultry cultivation and food industry etc..Clinically most antibiotic are for treating bacterium sense
Infectious diseases.The excessive use of antibiotic not only will produce superbacteria, while can also influence the detection of microorganism in blood.
Blood culture provides important diagnosis and treatment information for bloodstream infection patient, and the cure rate to improving bloodstream infection has important meaning.But
During blood culture, due to applying various extensive pedigree antibiotics in advance so that the bacterium in blood is by antibacterials
Inhibiting effect and be difficult to grow, cause culture false negative.Therefore in order to improve the positive rate of Full-automatic blood culture instrument, it is necessary to
Remove the interference of antibiotic in blood.
Cephalosporin analog antibiotic is one kind in clinical common antibiotics.Therefore, the removal problem of cephalosporin analog antibiotic is studied,
For solving the problems, such as that positive rate is low meaningful in blood culture.
The approach of removal antibiotic has very much, such as oxidizing process, biological treatment, absorption method etc..
(1) oxidizing process is mainly aoxidized using hydroxyl radical free radical as strong oxidizer or mineralising organic matter.
Hou etc. is in Ultrasound-assisted heterogeneous Fenton-like degradation of
H is used in mono- texts of tetracycline over a magnetite catalyst2O2Oxidation technology realizes in 1 hour
The removal rate of tetracycline 93.6%.
Michael etc. is in Fatta-Kassinos.Solar Fenton and solar TiO2catalytic
treatment of ofloxacin in secondary treated effluents:Evaluation of
TiO is utilized in mono- texts of operational and kinetic parameters.2Make oxidant removal Ofloxacin, removal rate
Up to 60%.
Although antibiotic medicine can be degraded to non-poisonous material by oxidizing process, in biological sample culture, due to oxygen
Agent cannot use the lethal effect of bacterium in biological sample culture.
(2) biological treatment includes mainly activated sludge process, membrane biological reaction facture.
Li B, Zhang T etc. is in Mass flows and removal of antibiotics in two
It proposes to utilize the bacterium pair in sludge in mono- texts of municipal wastewater treatment plants.Chemosphere
The decomposition of antibiotic, achievees the purpose that taking-up.
But due to introducing bacterium, this method cannot be used in biological sample culture.
(3) absorption method is to utilize some porous solid adsorption materials, makes one kind in solution phase or substance absorption
To solid phase surface, to realize the method efficiently separated to pollutant.
Sorbing material used in absorption method mainly has:Adsorb resin, ion exchange resin, amorphous carbon, primary amino-compound.
The combination of absorption resin and ion exchange resin described in two patents of US4,145,304 and US5,314,229
Only to penicillins (benzyl penicillin, ampicillin, methicillin), aminoglycoside (amikacin, gentamicin), big ring
The adsorption effect that the antibiosis such as lactone (erythromycin) and polyenoid class (anphotericin) have been known as does not give other classification antibiotic outstanding
It is the adsorption effect of cephalosporin analog antibiotic.
Amorphous carbon described in United States Patent (USP) 5,162,229 and United States Patent (USP) 8,911,962 is to beta-lactam antibiosis
Carbapenems and cephalosporin analog antibiotic adsorption effect in element is bad.
Primary amino-compound described in United States Patent (USP) 8,603,770 and United States Patent (USP) 8,420,346 is anti-to beta-lactam
Raw element (such as Carbapenems) has adsorption effect, and the absorption of other classification antibiotic especially cephalosporin analog antibiotics is not given to imitate
Fruit.
In conclusion there are no the methods of good removal cephalosporin analog antibiotic so far.
Invention content
The object of the present invention is to provide a kind of applications of porous carbon materials, and especially cephalosporin analog antibiotic adsorption applications are to carry
The positive rate of bacterium in high biological sample culture simplifies operation, improves efficiency, reduces energy consumption, reduces investment and pollution.
A kind of application of porous carbon materials of the present invention, the porous carbon materials adsorb the cephalo-type antibiosis in biological sample
Element；
The porous carbon materials have vesica shape macroporous structure；
There is macropore, micropore and/or mesoporous on the hole wall of the vesica shape macropore.
The preferred aperture of vesica shape macropore is 2-5 μm.
The preferred aperture of micropore is 0.4-1nm.
The microscopic appearance structures of the porous carbon materials as shown in Figure 1, its with vesica shape macroporous structure.
The diameter of the vesica shape macropore is not more than 5 μm.
There is macropore, micropore and/or mesoporous on the hole wall of the vesica shape macropore.
Macropore diameter on vesica shape macropore hole wall is not more than 500nm.
Inventors discovered through research that being had by using the porous carbon materials of specific morphology of the present invention and pore-size distribution
Some vesica shape macroporous structures keep it apparent to the adsorption effect of cephalosporin analog antibiotic with hierarchical porous structure and its pore-size distribution.
Fig. 2 is the EDS of the porous carbon materials.By EDS elemental analyses, other than carbon, also aerobic, silicon, sodium,
The elements such as calcium, magnesium.It can be obtained from the elemental analysis table that table 1 is porous carbon materials, carbon has accounted for 88.47%, and oxygen element accounts for
10.37%, it is other to have a small amount of silicon, sodium, calcium, magnesium elements.Wherein, a small amount of sodium element may be from sodium hydroxide activation
Agent, remaining element may be from rice husk matrix.
The elemental analysis table of 1. porous carbon materials of table
| Element | C | O | Other |
| Mass percent | 88.47 | 10.37 | 1.16 |
Fig. 3 is that the BET of the porous carbon materials schemes.It can be seen from the figure that the specific surface area S of the porous carbon materialsm=
1192m2/g。
The porous carbon materials are prepared using biomass.
The biomass includes rice husk.
The preparation of the porous carbon materials includes the following steps：
(1) it is carbonized：After rice husk is kept the temperature carbonization at 100~500 DEG C, 80 mesh sieve is crossed in grinding；
(2) activation heat treatment：Rice husk after carbonization is mixed with sodium hydroxide, in 600~900 DEG C, in nitrogen or inertia
After cooling being kept the temperature under the protection of atmosphere, washing；200 mesh sieve is crossed in drying grinding.
Rice husk is placed in crucible, is carbonized in Muffle furnace.Rice husk carbonizing temperature is preferably 300~500 DEG C.Rice husk
Carbonization time at least 1 hour.
Carbonization rice husk is kept the temperature in being mixed in crucible in Muffle furnace with sodium hydroxide.Soaking time is preferably 2~4h.
The mass ratio of the carbonization rice husk and sodium hydroxide is not higher than 10：1.
The biological sample includes blood, blood plasma, one kind in serum and saliva.
The cephalosporin analog antibiotic includes：Ceftriaxone Sodium, cefotaxime, Cefepime, cefoperazone sodium sulbactam sodium
One or more of.
The porous carbon materials are mixed with culture medium, adsorb cephalosporin analog antibiotic, in 121 DEG C of sterilizings；
Biological sample is added in the culture medium under sterile conditions, constant temperature incubation.
Inventor prepares porous carbon materials and culture medium is sequentially placed into culture bottle and mixes certainly by described.And at 121 DEG C
Carry out high-temperature sterilization.It then cools to room temperature.Biological sample is added in the culture bottle of above-mentioned cooling with sterile working, is carried out
Constant temperature incubation.The piezoelectricity Full-automatic blood culture instrument of the Publication No. CN101403724A disclosed in Chinese patent literature carries out entirely certainly
Dynamic on-line checking.
If instrument report sun, show that antibiotic has been adsorbed onto Mlc hereinafter, result is used by such porous carbon materials
Positive is indicated；If instrument report is cloudy, as a result indicated with Negative.Testing result such as table 2, the results showed that more described in inventing
Hole carbon material quickly and effectively can adsorb and/or neutralize the antibiotic in biological sample, and especially cephalosporin analog antibiotic, raising is faced
The positive rate of bed microorganism.
2 antibiotic blood culture of table detects situation
Beneficial effects of the present invention are as follows：
For the application compared with the existing technology of porous carbon materials provided by the invention, it will not kill thin in biological sample
Bacterium will not introduce alien bacteria, and easy to operate, efficient, low energy consumption, and investment cost is low, does not generate secondary pollution, will not band
Come toxicity bigger or pollutant more difficult to degrade.The alternative efficient absorption cephalosporin analog antibiotic of the porous carbon materials, can
Significantly improve the positive rate of bacterium in biological sample culture.
Description of the drawings
The SEM appearance structure figures of Fig. 1 porous carbon materials
The EDS of Fig. 2 porous carbon materials schemes
Fig. 3 is that the BET of porous carbon materials schemes
Fig. 4 is relative phase shift (△ F)-time (t) curve of the blank control bottle of embodiment 2.
Fig. 5 is relative phase shift (△ F)-time (t) curve of the porous carbon materials bottle of embodiment 2.
Fig. 6 is relative phase shift (△ F)-time (t) curve of the porous carbon materials bottle of embodiment 3.
Fig. 7 is relative phase shift (△ F)-time (t) curve of the porous carbon materials bottle of embodiment 3.
Fig. 8 is relative phase shift (△ F)-time (t) curve of the porous carbon materials bottle of embodiment 4.
Fig. 9 is relative phase shift (△ F)-time (t) curve of the porous carbon materials bottle of embodiment 4.
Figure 10 is relative phase shift (△ F)-time (t) curve of the porous carbon materials bottle of embodiment 5.
Figure 11 is relative phase shift (△ F)-time (t) curve of the porous carbon materials bottle of embodiment 5.
Figure 12 is relative phase shift (△ F)-time (t) curve of the porous carbon materials bottle of embodiment 6.
Figure 13 is relative phase shift (△ F)-time (t) curve of the porous carbon materials bottle of embodiment 6.
Figure 14 is relative phase shift (△ F)-time (t) curve of the porous carbon materials bottle of embodiment 7.
Figure 15 is relative phase shift (△ F)-time (t) curve of the porous carbon materials bottle of embodiment 7.
Figure 16 is relative phase shift (△ F)-time (t) curve of the porous carbon materials bottle of embodiment 8.
Figure 17 is relative phase shift (△ F)-time (t) curve of the porous carbon materials bottle of embodiment 8.
Figure 18 is relative phase shift (△ F)-time (t) curve of the blank control bottle of embodiment 9.
Figure 19 is relative phase shift (△ F)-time (t) curve of the porous carbon materials bottle of embodiment 9.
Figure 20 is relative phase shift (△ F)-time (t) curve of the porous carbon materials bottle of embodiment 10.
Figure 21 is relative phase shift (△ F)-time (t) curve of the porous carbon materials bottle of embodiment 10.
Figure 22 is relative phase shift (△ F)-time (t) curve of the blank control bottle of embodiment 11.
Figure 23 is relative phase shift (△ F)-time (t) curve of the porous carbon materials bottle of embodiment 11.
Figure 24 is relative phase shift (△ F)-time (t) curve of the porous carbon materials bottle of embodiment 12.
Figure 25 is relative phase shift (△ F)-time (t) curve of the porous carbon materials bottle of embodiment 12.
Figure 26 is the SEM appearance structure figures of porous carbon materials prepared by wood chip.
Figure 27 is the SEM appearance structure figures of porous carbon materials prepared by coconut husk.
Specific implementation mode
It is as follows with the antibiotic method and step in the porous carbon materials absorption blood：
Step (1) prepares porous carbon materials and culture medium is sequentially placed into culture bottle and mixes certainly by described, and at 121 DEG C
Lower carry out high-temperature sterilization.
Biological sample is added to culture bottle cooling in step (1) by step (2) with sterile working, carries out constant temperature incubation.
Step (3) with the piezoelectricity Full-automatic blood culture instrument of Publication No. CN101403724A disclosed in Chinese patent literature into
The full-automatic on-line checking of row.
If instrument report sun, show that antibiotic has been adsorbed onto Mlc hereinafter, result is used by such porous carbon materials
Positive is indicated；If instrument report is cloudy, as a result indicated with Negative.
Embodiment 1
Absorption of the porous carbon materials to antibiotic
Further include with the side for adsorbing antibiotic, especially cephalosporin analog antibiotic from the porous carbon materials prepared in the present invention
Method.It is specific as follows：
40mL systems include the porous carbon materials of the above-mentioned preparations of 3.0g, the Ceftriaxone Sodium or 4.8mg cefotaximes of 10mg
Or the deionized water of 6.56mg Cefepimes or 7.12mg cefoperazone sodiums-sulbactam and 40mL.After standing 24H, take respectively
Supernatant, and be filled into 5mL centrifuge tubes, it marks, ultraviolet-uisible spectrophotometer is used in combination that detection is gone to obtain successively molten
Liquid.Again respectively with the Ceftriaxone Sodium of 1 μ g/mL, 4 μ g/mL cefotaximes, 8 μ g/mL Cefepimes, 4 μ g/mL cefoperazone sodiums-
The ultraviolet curve of sulbactam solution compares.The results are shown in Table 3.
Table 3：Influence of the porous carbon materials to antibiotic adsorption effect in water
As can be known from Table 2：By processing for 24 hours, Ceftriaxone Sodium, head after being adsorbed with spectrophotometry in solution
Spore he pyridine, Cefepime and cefoperazone sodium-sulbactam are not detected.
Embodiment 2
The porous carbon materials of preparation are used for the detection of Full-automatic blood culture instrument.Full-automatic Blood culture system used is pressure
Electric Full-automatic blood culture instrument (Chinese patent literature with reference to Publication No. CN101403724A discloses).Full-automatic blood culture instrument is matched
Cover the preparation of Blood culture bottle：1. without porous carbon materials bottle (blank control bottle is not added with the porous carbon materials) culture bottle
Middle addition 38mL culture mediums (beef extract 2g/L；Yeast extract 1g/L；Glucose 11g/L；18AA amino acid 1 6mL/L, anti-coagulants
(sodium polyanethole sulfonate) 0.25g/L, pH 7.2, deionized water)；2. 38mL cultures are added in porous carbon materials bottle culture bottle
Base (beef extract 2g/L；Yeast extract 1g/L；Glucose 11g/L；18AA amino acid 1 6mL/L, anti-coagulants (poly anetholesulfonic acid
Sodium) 0.25g/L, pH 7.2, deionized water) and porous carbon materials.All culture bottles are both needed to cover bottle stopper to go out through 121 DEG C of high temperature
It is used after bacterium pot sterilizing cooling in 30 minutes.
Simulate the preparation of blood sample：The sterile Sheep Bloods of 1mL, 1.0ml * 10 are added into the above-mentioned culture bottle prepared4cfu/
The bacterium solution (Escherichia coli, staphylococcus aureus or pseudomonas aeruginosa, according to the corresponding bacterium solution of different selections of antibiotic) of mL,
Blood concentration antibiotic (see specific embodiment) covers bottle stopper oscillation and shakes up.The conduct experimental group for adding porous carbon materials, is not added
The conduct blank control group of hole carbon material.Every group do 8 it is parallel.
Simulate the detection of blood sample：Culture bottle equipped with sample to be tested is put into piezoelectricity Full-automatic blood culture instrument (Publication No.
CN101403724A in detect tank), 37 DEG C of constant temperature incubations.Corresponding software on computer is opened, the conductance of solution becomes in culture bottle
Change by real time on-line monitoring and can automatically record corresponding relative phase shift (△ F)-time (t) curve.Testing result is shown in specifically
Embodiment, response curve are shown in description of the drawings book.
The sterile Sheep Bloods of 1mL, 1.0ml*10 are sequentially added into the above-mentioned culture bottle prepared4The golden yellow Portugal of cfu/mL
Grape coccus, antibiotic are the cefotaxime (a concentration of 120 μ g/ml in culture bottle) of cephalo-type, cover bottle stopper oscillation and shake up.It adds
The conduct experimental group of hole carbon material does not add the conduct blank control group of porous carbon materials.Every group do 8 it is parallel.Culture bottle is put
In the detect tank for entering piezoelectricity Full-automatic blood culture instrument (Publication No. CN101403724A), 37 DEG C of constant temperature incubations, in 120 hours
Sun is not reported to be then no longer detected.
Testing result is shown in Fig. 4 and Fig. 5.Fig. 4 is relative phase shift (△ F)-time (t) curve of blank control bottle, display
The testing result of microorganism (staphylococcus aureus) in blank control bottle is negative；Fig. 5 is the opposite of porous carbon materials bottle
Frequency displacement (△ F)-time (t) curve shows that the testing result of the microorganism (staphylococcus aureus) in porous carbon materials bottle is in
It is positive.
Embodiment 3
The sterile Sheep Bloods of 1mL, 1.0ml*10 are sequentially added into the above-mentioned culture bottle prepared4The golden yellow Portugal of cfu/mL
Grape coccus, antibiotic are the Cefepime (a concentration of 164 μ g/ml in culture bottle) of cephalo-type, cover bottle stopper oscillation and shake up.It adds
The conduct experimental group of hole carbon material does not add the conduct blank control group of porous carbon materials.Every group do 8 it is parallel.Culture bottle is put
In the detect tank for entering piezoelectricity Full-automatic blood culture instrument (Publication No. CN101403724A), 37 DEG C of constant temperature incubations, in 120 hours
Sun is not reported to be then no longer detected.
Testing result is shown in Fig. 6 and Fig. 7.Fig. 6 is relative phase shift (△ F)-time (t) curve of blank control bottle, display
The testing result of microorganism (staphylococcus aureus) in blank control bottle is negative；Fig. 7 is the opposite of porous carbon materials bottle
Frequency displacement (△ F)-time (t) curve shows that the testing result of the microorganism (staphylococcus aureus) in porous carbon materials bottle is in
It is positive.
Embodiment 4
The sterile Sheep Bloods of 1mL, 1.0ml*10 are sequentially added into the above-mentioned culture bottle prepared4The golden yellow Portugal of cfu/mL
Grape coccus, antibiotic are the Ceftriaxone Sodium (a concentration of 250 μ g/ml in culture bottle) of cephalo-type, cover bottle stopper oscillation and shake up.Add
The conduct experimental group of porous carbon materials does not add the conduct blank control group of porous carbon materials.Every group do 8 it is parallel.By culture bottle
It is put into the detect tank of piezoelectricity Full-automatic blood culture instrument (Publication No. CN101403724A), 37 DEG C of constant temperature incubations, 120 hours
Sun is not reported inside to be then no longer detected.
Testing result is shown in Fig. 8 and Fig. 9.Fig. 8 is relative phase shift (△ F)-time (t) curve of blank control bottle, display
The testing result of microorganism (staphylococcus aureus) in blank control bottle is negative；Fig. 9 is the opposite of porous carbon materials bottle
Frequency displacement (△ F)-time (t) curve shows that the testing result of the microorganism (staphylococcus aureus) in porous carbon materials bottle is in
It is positive.
Embodiment 5
The sterile Sheep Bloods of 1mL, 1.0ml*10 are sequentially added into the above-mentioned culture bottle prepared4The large intestine bar of cfu/mL
Bacterium, antibiotic are the Ceftriaxone Sodium (a concentration of 250 μ g/ml in culture bottle) of cephalo-type, cover bottle stopper oscillation and shake up.Add hole
The conduct experimental group of carbon material does not add the conduct blank control group of porous carbon materials.Every group do 8 it is parallel.Culture bottle is put into
In the detect tank of piezoelectricity Full-automatic blood culture instrument (Publication No. CN101403724A), 37 DEG C of constant temperature incubations, in 120 hours not
Report sun is then no longer detected.
Testing result is shown in Figure 10 and Figure 11.Figure 10 is relative phase shift (△ F)-time (t) curve of blank control bottle, is shown
Show that the testing result of the microorganism (Escherichia coli) in blank control bottle is negative；Figure 11 is the relative phase shift of porous carbon materials bottle
(△ F)-time (t) curve shows that the testing result of the microorganism (Escherichia coli) in porous carbon materials bottle is positive.
Embodiment 6
The sterile Sheep Bloods of 1mL, 1.0ml*10 are sequentially added into the above-mentioned culture bottle prepared4The large intestine bar of cfu/mL
Bacterium, antibiotic are the cefotaxime (a concentration of 120 μ g/ml in culture bottle) of cephalo-type, cover bottle stopper oscillation and shake up.Add porous carbon
The conduct experimental group of material does not add the conduct blank control group of porous carbon materials.Every group do 8 it is parallel.Culture bottle is put into pressure
In the detect tank of electric Full-automatic blood culture instrument (Publication No. CN101403724A), 37 DEG C of constant temperature incubations are not reported in 120 hours
It is positive then be no longer detected.
Testing result is shown in Figure 12 and Figure 13.Figure 12 is relative phase shift (△ F)-time (t) curve of blank control bottle, is shown
Show that the testing result of the microorganism (Escherichia coli) in blank control bottle is negative；Figure 13 is the relative phase shift of porous carbon materials bottle
(△ F)-time (t) curve shows that the testing result of the microorganism (Escherichia coli) in porous carbon materials bottle is positive.
Embodiment 7
The sterile Sheep Bloods of 1mL, 1.0ml*10 are sequentially added into the above-mentioned culture bottle prepared4The large intestine bar of cfu/mL
Bacterium, antibiotic are the Cefepime (a concentration of 164 μ g/ml in culture bottle) of cephalo-type, cover bottle stopper oscillation and shake up.Add porous carbon
The conduct experimental group of material does not add the conduct blank control group of porous carbon materials.Every group do 8 it is parallel.Culture bottle is put into pressure
In the detect tank of electric Full-automatic blood culture instrument (Publication No. CN101403724A), 37 DEG C of constant temperature incubations are not reported in 120 hours
It is positive then be no longer detected.
Testing result is shown in Figure 14 and Figure 15.Figure 14 is relative phase shift (△ F)-time (t) curve of blank control bottle, is shown
Show that the testing result of the microorganism (Escherichia coli) in blank control bottle is negative；Figure 15 is the relative phase shift of porous carbon materials bottle
(△ F)-time (t) curve shows that the testing result of the microorganism (Escherichia coli) in porous carbon materials bottle is positive.
Embodiment 8
The sterile Sheep Bloods of 1mL, 1.0ml*10 are sequentially added into the above-mentioned culture bottle prepared4The large intestine bar of cfu/mL
Bacterium, antibiotic are the cefoperazone sodium and sulbactam (a concentration of 53 μ g/ml in culture bottle) of cephalo-type, cover bottle stopper oscillation and shake
It is even.Add the conduct experimental group of porous carbon materials, does not add the conduct blank control group of porous carbon materials.Every group do 8 it is parallel.It will training
Foster bottle is put into the detect tank of piezoelectricity Full-automatic blood culture instrument (Publication No. CN101403724A), 37 DEG C of constant temperature incubations, and 120
Sun is not reported to be then no longer detected in hour.
Testing result is shown in Figure 16 and Figure 17.Figure 16 is relative phase shift (△ F)-time (t) curve of blank control bottle, is shown
Show that the testing result of the microorganism (Escherichia coli) in blank control bottle is negative；Figure 17 is the relative phase shift of porous carbon materials bottle
(△ F)-time (t) curve shows that the testing result of the microorganism (Escherichia coli) in porous carbon materials bottle is positive.
Embodiment 9
The sterile Sheep Bloods of 1mL, 1.0ml*10 are sequentially added into the above-mentioned culture bottle prepared4The large intestine bar of cfu/mL
Bacterium, antibiotic are the cefoperazone sodium and sulbactam (a concentration of 178 μ g/ml in culture bottle) of cephalo-type, cover bottle stopper oscillation
It shakes up.Add the conduct experimental group of porous carbon materials, does not add the conduct blank control group of porous carbon materials.Every group do 8 it is parallel.It will
Culture bottle is put into the detect tank of piezoelectricity Full-automatic blood culture instrument (Publication No. CN101403724A), 37 DEG C of constant temperature incubations,
Sun is not reported to be then no longer detected in 120 hours.
Testing result is shown in Figure 18 and Figure 19.Figure 18 is relative phase shift (△ F)-time (t) curve of blank control bottle, is shown
Show that the testing result of the microorganism (Escherichia coli) in blank control bottle is negative；Figure 19 is the relative phase shift of porous carbon materials bottle
(△ F)-time (t) curve shows that the testing result of the microorganism (Escherichia coli) in porous carbon materials bottle is positive.
Embodiment 10
The sterile Sheep Bloods of 1mL, 1.0ml*10 are sequentially added into the above-mentioned culture bottle prepared4The verdigris of cfu/mL is false single
Born of the same parents bacterium, antibiotic are the Cefepime (a concentration of 164 μ g/ml in culture bottle) of cephalo-type, cover bottle stopper oscillation and shake up.Add hole
The conduct experimental group of carbon material does not add the conduct blank control group of porous carbon materials.Every group do 8 it is parallel.Culture bottle is put into
In the detect tank of piezoelectricity Full-automatic blood culture instrument (Publication No. CN101403724A), 37 DEG C of constant temperature incubations, in 120 hours not
Report sun is then no longer detected.
Testing result is shown in Figure 20 and Figure 21.Figure 20 is relative phase shift (△ F)-time (t) curve of blank control bottle, is shown
Show that the testing result of the microorganism (pseudomonas aeruginosa) in blank control bottle is negative；Figure 21 is the phase of porous carbon materials bottle
To frequency displacement (△ F)-time (t) curve, show that the testing result of the microorganism (pseudomonas aeruginosa) in porous carbon materials bottle is in
It is positive.
Embodiment 11
The sterile Sheep Bloods of 1mL, 1.0ml*10 are sequentially added into the above-mentioned culture bottle prepared4The verdigris of cfu/mL is false single
Born of the same parents bacterium, antibiotic are the cefotaxime (a concentration of 120 μ g/ml in culture bottle) of cephalo-type, cover bottle stopper oscillation and shake up.Add hole
The conduct experimental group of carbon material does not add the conduct blank control group of porous carbon materials.Every group do 8 it is parallel.Culture bottle is put into
In the detect tank of piezoelectricity Full-automatic blood culture instrument (Publication No. CN101403724A), 37 DEG C of constant temperature incubations, in 120 hours not
Report sun is then no longer detected.
Testing result is shown in Figure 22 and Figure 23.Figure 22 is relative phase shift (△ F)-time (t) curve of blank control bottle, is shown
Show that the testing result of the microorganism (pseudomonas aeruginosa) in blank control bottle is negative；Figure 23 is the phase of porous carbon materials bottle
To frequency displacement (△ F)-time (t) curve, show that the testing result of the microorganism (pseudomonas aeruginosa) in porous carbon materials bottle is in
It is positive.
Embodiment 12
The sterile Sheep Bloods of 1mL, 1.0ml*10 are sequentially added into the above-mentioned culture bottle prepared4The verdigris of cfu/mL is false single
Born of the same parents bacterium, antibiotic are the Ceftriaxone Sodium (a concentration of 250 μ g/ml in culture bottle) of cephalo-type, cover bottle stopper oscillation and shake up.It adds
The conduct experimental group of hole carbon material does not add the conduct blank control group of porous carbon materials.Every group do 8 it is parallel.Culture bottle is put
In the detect tank for entering piezoelectricity Full-automatic blood culture instrument (Publication No. CN101403724A), 37 DEG C of constant temperature incubations, in 120 hours
Sun is not reported to be then no longer detected.
Testing result is shown in Figure 24 and Figure 25.Figure 24 is relative phase shift (△ F)-time (t) curve of blank control bottle, is shown
Show that the testing result of the microorganism (pseudomonas aeruginosa) in blank control bottle is negative；Figure 25 is the phase of porous carbon materials bottle
To frequency displacement (△ F)-time (t) curve, show that the testing result of the microorganism (pseudomonas aeruginosa) in porous carbon materials bottle is in
It is positive.
Comparative example 1
Porous carbon materials are prepared by raw material of wood chip, are as follows：
The wood chip obtained by torch pine, by the mass ratio 1 of wood chip and KOH：4, impregnating active 96 hours.Then by wood chip mistake
Filter air-dries and at 850 DEG C in N23 hours KOH with activation dipping are pyrolyzed in atmosphere.Material is ground using mortar and pestle
At fine powder, other pyrolysis step is then carried out in argon gas at 600 DEG C 2 hours to clean sample and remove impurity.Most
Afterwards, air-activated 6 hours at 300 DEG C, it is as shown in figure 26 to obtain carbon material microscopic appearance, only has level-one hole.Empirically
Method test described in example 1, is found through experiments that the activated carbon is bad to the adsorption effect of cephalosporin analog antibiotic.
Comparative example 2
Porous carbon materials are prepared by raw material of coconut husk, are as follows：
Coconut husk is obtained into carbon material under the atmosphere of vapor and carbon dioxide in 800-1000 DEG C of carbonization.
The carbon material microscopic appearance prepared by coconut husk is as shown in figure 27, only has level-one hole, does not have the work of the present invention
Special construction possessed by property charcoal.The empirically method test described in example 1, is found through experiments that the absorption to cephalosporin analog antibiotic
It is ineffective.
Claims (10)
1. a kind of application of porous carbon materials, it is characterised in that：The porous carbon materials are used to adsorb the cephalo in biological sample
Class antibiotic；
The porous carbon materials have vesica shape macroporous structure；
There is macropore, micropore and/or mesoporous on the hole wall of the vesica shape macropore.
2. the application of porous carbon materials as described in claim 1, it is characterised in that：The diameter of the vesica shape macropore is not more than
5μm；The aperture of the preferably described vesica shape macropore is 2-5 μm.
3. the application of porous carbon materials as described in claim 1, it is characterised in that：The aperture of the micropore is 0.4-1nm.
4. the application of porous carbon materials as described in claim 1, it is characterised in that：Macropore diameter on vesica shape macropore hole wall
No more than 500nm.
5. the application of porous carbon materials as described in claim 1, it is characterised in that：The porous carbon materials are by biomass system
It is standby to form.
6. the application of porous carbon materials as claimed in claim 5, it is characterised in that：The porous carbon materials are passed through by biomass
Carbonization, alkali activation heat treatment form.
7. the application of porous carbon materials as described in claim 1, it is characterised in that：The biomass is rice husk.
8. the application of porous carbon materials as claimed in claim 7, it is characterised in that：The preparation of the porous carbon materials include with
Lower step：
(1) it is carbonized：After rice husk is kept the temperature carbonization at 100~500 DEG C, 80 mesh sieve is crossed in grinding；
(2) alkali activation heat treatment：Rice husk after carbonization is mixed with sodium hydroxide, in 600~900 DEG C, in nitrogen or indifferent gas
After cooling being kept the temperature under the protection of atmosphere, washing；200 mesh sieve is crossed in drying grinding.
9. the application of porous carbon materials as claimed in claim 8, it is characterised in that：The matter of the carbonization rice husk and sodium hydroxide
Amount is than being not higher than 10：1.
10. the application of porous carbon materials as described in claim 1, it is characterised in that：The biological sample includes blood, blood
It starches, one kind in serum and saliva.
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